Addgene pOPINVHH_H6_His Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pOPINVHH_H6_His Sequencing Result


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BanII (4178) BtgZI (4104) BsaAI - DraIII (4103) F1ori-F (4016 .. 4037) PsiI (3975) BbsI (3841) BmtI (3773) NheI (3769) AflII (3764) NdeI (3639) BspDI - ClaI (3443) BfuAI - BspMI (3182) BamHI (3135) BsmI (2667) BstXI (2503) BsmBI - Bsu36I - Esp3I (2465) BstEII (2453) PshAI (2451) KflI - PpuMI (2446) SpeI (2262) BsaBI (2254) PasI (2211) PluTI (2181) SfoI (2179) NarI (2178) KasI (2177) F1ori-R (4228 .. 4247) Amp-R (344 .. 363) ScaI (432) PvuI (544) FspI (690) NmeAIII (766) BpmI (844) BmrI (873) AhdI (913) pBR322ori-F (1646 .. 1665) NspI (1805) CAP binding site EcoRI (2004) NotI (2047) BglI - SfiI (2079) NcoI - StyI (2083) pOPINVHH_H6_His 4340 bp

Sequence View

60 AmpR promoter g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t

120 AmpR promoter a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t

180 1 5 10 15 signal sequence MSIQHFRVALIPFFAAFC AmpR promoter AmpR MSIQHFRVALIPFFAAFC a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g

240 20 signal sequence LPVFA 25 30 35 HPETLVKVKDAEDQL AmpR LPVFAHPETLVKVKDAEDQL t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c

300 40 45 50 55 GARVGYIELDLNSGKILESF AmpR GARVGYIELDLNSGKILESF g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g

360 60 65 70 75 RPEERFPMMSTFKVLLCGAV AmpR C G A T A C A C C G C G C C A T A Amp-R RPEERFPMMSTFKVLLCGAV g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a

420 80 85 90 95 LSRIDAGQEQLGRRIHYSQN AmpR A T A Amp-R LSRIDAGQEQLGRRIHYSQN t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c

ScaI 480 100 105 110 115 DLVEYSPVTEKHLTDGMTVR AmpR DLVEYSPVTEKHLTDGMTVR a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c

540 120 125 130 135 ELCSAAITMSDNTAANLLLT AmpR ELCSAAITMSDNTAANLLLT a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t

PvuI 600 140 145 150 155 TIGGPKELTAFLHNMGDHVT AmpR TIGGPKELTAFLHNMGDHVT c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g

660 160 165 170 175 RLDRWEPELNEAIPNDERDT AmpR RLDRWEPELNEAIPNDERDT g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t

FspI 720 180 185 190 195 TMPVAMATTLRKLLTGELLT AmpR TMPVAMATTLRKLLTGELLT c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g

NmeAIII 780 200 205 210 215 LASRQQLIDWMEADKVAGPL AmpR LASRQQLIDWMEADKVAGPL t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g

840 220 225 230 235 LRSALPAGWFIADKSGAGER AmpR LRSALPAGWFIADKSGAGER t g c g c t c g g c g c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g a c g c g a g c c g c g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c

BmrIBpmI 900 240 245 250 255 GSRGIIAALGPDGKPSRIVV AmpR GSRGIIAALGPDGKPSRIVV g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t

AhdI 960 260 265 270 275 IYTTGSQATMDERNRQIAEI AmpR IYTTGSQATMDERNRQIAEI t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c

1020 280 285 GASLIKHW* AmpR GASLIKHW* g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t

1080 t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g

1140 t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t

1200 ori a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t

1260 ori a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g

1320 ori c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a

1380 ori a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g

1440 ori t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g

1500 ori g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t

1560 ori g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c

1620 ori c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c

1680 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a

1740 ori t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a

1800 ori g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g

NspI 1860 a c a t g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t t g t a c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a

1920 CAP binding site g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a

1980 -35 -10 CAP binding site lac promoter a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c

EcoRI 2040 1 5 MKYLL lac operator pelB signal sequence MKYLL g a a t t g t g a g c g g a t a a c a a t t t g a a t t c a a g g a g a c a g t c a t a a t g a a a t a c c t a t t g c c t t a a c a c t c g c c t a t t g t t a a a c t t a a g t t c c t c t g t c a g t a t t a c t t t a t g g a t a a c g

NcoIStyIBglISfiINotI 2100 10 15 20 PTAAAGLLLLAAQPAMA QVQ pelB signal sequence PTAAAGLLLLAAQPAMAQVQ c t a c g g c g g c c g c t g g a t t g t t a t t a c t c g c g g c c c a g c c g g c c a t g g c c c a g g t g c a g c g a t g c c g c c g g c g a c c t a a c a a t a a t g a g c g c c g g g t c g g c c g g t a c c g g g t c c a c g t c g

2160 (in frame with pelB signal sequence) LVESGGGLVQPGGSLTLSCV LVESGGGLVQPGGSLTLSCV t g g t c g a g t c t g g g g g a g g c t t g g t g c a g c c t g g g g g g t c t c t g a c a c t c t c c t g t g t a g a c c a g c t c a g a c c c c c t c c g a a c c a c g t c g g a c c c c c c a g a g a c t g t g a g a g g a c a c a t c

PasIKasINarISfoIPluTI 2220 (in frame with pelB signal sequence) ASESSLAPYRVAWFRQAPGK ASESSLAPYRVAWFRQAPGK c g t c g g a a t c a t c t t t g g c g c c t t a t c g c g t a g c c t g g t t c c g t c a g g c c c c a g g g a a g g g c a g c c t t a g t a g a a a c c g c g g a a t a g c g c a t c g g a c c a a g g c a g t c c g g g g t c c c t t c c

SpeIBsaBI 2280 (in frame with pelB signal sequence) EREGVSCISRDAHPTSTYYT EREGVSCISRDAHPTSTYYT a g c g c g a g g g g g t c t c a t g t a t t a g t c g t g a t g c a c a t c c t a c t a g t a c a t a c t a t a c a g t c g c g c t c c c c c a g a g t a c a t a a t c a g c a c t a c g t g t a g g a t g a t c a t g t a t g a t a t g t c

2340 (in frame with pelB signal sequence) ASVKGRFTMSRDNAKNTVYL ASVKGRFTMSRDNAKNTVYL c c t c c g t g a a g g g c c g a t t c a c c a t g t c c a g a g a c a a t g c c a a g a a c a c g g t g t a t c t g c g g a g g c a c t t c c c g g c t a a g t g g t a c a g g t c t c t g t t a c g g t t c t t g t g c c a c a t a g a c g

2400 (in frame with pelB signal sequence) QMNSLKPSDTAVYYCATDLG QMNSLKPSDTAVYYCATDLG a a a t g a a c a g c c t g a a a c c a t c g g a c a c g g c c g t t t a t t a c t g t g c g a c a g a t t t g g g a g t t t a c t t g t c g g a c t t t g g t a g c c t g t g c c g g c a a a t a a t g a c a c g c t g t c t a a a c c c t c

PpuMIKflIPshAIBstEII 2460 (in frame with pelB signal sequence) GYCSDSNYPRAWWGQGTQVT GYCSDSNYPRAWWGQGTQVT g a t a c t g t t c g g a t t c t a a t t a t c c c c g c g c c t g g t g g g g c c a g g g g a c c c a g g t c a c c g c t a t g a c a a g c c t a a g a t t a a t a g g g g c g c g g a c c a c c c c g g t c c c c t g g g t c c a g t g g c

BstXIBsu36IBsmBIEsp3I 2520 VSSG 1 5 HHHHHH * 1 EQK 6xHis Myc VSSGHHHHHH* t c t c c t c a g g c c a c c a t c a c c a t c a c c a t t a a t a a c c a c c a t c a t g g c g c a g a a c a a a a a a g a g g a g t c c g g t g g t a g t g g t a g t g g t a a t t a t t g g t g g t a g t a c c g c g t c t t g t t t t t

2580 5 10 LISEEDL S* Myc c t c a t c t c a g a a g a g g a t c t g t c t t a g g c c g a a a c t g t t g a a a g t t g t t t a g c a a a a c c t g a g t a g a g t c t t c t c c t a g a c a g a a t c c g g c t t t g a c a a c t t t c a a c a a a t c g t t t t g g a

2640 c a t a c a g a a a a t t c a t t t a c t a a c g t c t g g a a a g a c g a c a a a a c t t t a g a t c g t t a c g c t g t a t g t c t t t t a a g t a a a t g a t t g c a g a c c t t t c t g c t g t t t t g a a a t c t a g c a a t g c g a

BsmI 2700 a a c t a t g a g g g c t g t c t g t g g a a t g c t a c a g g c g t t g t g g t t t g t a c t g g t g a c g a a a c t t t g a t a c t c c c g a c a g a c a c c t t a c g a t g t c c g c a a c a c c a a a c a t g a c c a c t g c t t t g a

2760 c a g t g t t a c g g t a c a t g g g t t c c t a t t g g g c t t g c t a t c c c t g a a a a t g a g g g t g g t g g c g t c a c a a t g c c a t g t a c c c a a g g a t a a c c c g a a c g a t a g g g a c t t t t a c t c c c a c c a c c g

2820 t c t g a g g g t g g c g g t t c t g a g g g t g g c g g t t c t g a g g g t g g c g g t a c t a a a c c t c c t g a g a g a c t c c c a c c g c c a a g a c t c c c a c c g c c a a g a c t c c c a c c g c c a t g a t t t g g a g g a c t c

2880 t a c g g t g a t a c a c c t a t t c c g g g c t a t a c t t a t a t c a a c c c t c t c g a c g g c a c t t a t c c g a t g c c a c t a t g t g g a t a a g g c c c g a t a t g a a t a t a g t t g g g a g a g c t g c c g t g a a t a g g c

2940 c c t g g t a c t g a g c a a a a c c c c g c t a a t c c t a a t c c t t c t c t t g a g g a g t c t c a g c c t c t t g g a c c a t g a c t c g t t t t g g g g c g a t t a g g a t t a g g a a g a g a a c t c c t c a g a g t c g g a g a a

3000 MFQNNRFRNRQGALTVY a a t a c t t t c a t g t t t c a g a a t a a t a g g t t c c g a a a t a g g c a g g g t g c a t t a a c t g t t t a t t t a t g a a a g t a c a a a g t c t t a t t a t c c a a g g c t t t a t c c g t c c c a c g t a a t t g a c a a a t a

3060 TGTVTQGTDPVKTYYQYTPV a c g g g c a c t g t t a c t c a a g g c a c t g a c c c c g t t a a a a c t t a t t a c c a g t a c a c t c c t g t a t g c c c g t g a c a a t g a g t t c c g t g a c t g g g g c a a t t t t g a a t a a t g g t c a t g t g a g g a c a t

3120 SSKAMYDAYWNGKFRDCAFH t c a t c a a a a g c c a t g t a t g a c g c t t a c t g g a a c g g t a a a t t c a g a g a c t g c g c t t t c c a t a g t a g t t t t c g g t a c a t a c t g c g a a t g a c c t t g c c a t t t a a g t c t c t g a c g c g a a a g g t a

BamHI 3180 SGFNEDPFVCEYQGQSSDLP t c t g g c t t t a a t g a g g a t c c a t t c g t t t g t g a a t a t c a a g g c c a a t c g t c t g a c c t g c c t a g a c c g a a a t t a c t c c t a g g t a a g c a a a c a c t t a t a g t t c c g g t t a g c a g a c t g g a c g g a

BspMIBfuAI 3240 QPPVNAGGGSGGGSGGGSEG c a a c c t c c t g t c a a t g c t g g c g g c g g c t c t g g t g g t g g t t c t g g t g g c g g c t c t g a g g g t g t t g g a g g a c a g t t a c g a c c g c c g c c g a g a c c a c c a c c a a g a c c a c c g c c g a g a c t c c c a

3300 GGSEGGGSEGGGSEGGGSGG g g c g g c t c t g a g g g t g g c g g t t c t g a g g g t g g c g g c t c t g a g g g t g g c g g t t c c g g t g g c c c g c c g a g a c t c c c a c c g c c a a g a c t c c c a c c g c c g a g a c t c c c a c c g c c a a g g c c a c c g

3360 GSGSGDFDYEKMANANKGAM g g c t c c g g t t c c g g t g a t t t t g a t t a t g a a a a a a t g g c a a a c g c t a a t a a g g g g g c t a t g c c g a g g c c a a g g c c a c t a a a a c t a a t a c t t t t t t a c c g t t t g c g a t t a t t c c c c c g a t a c

3420 TENADENALQSDAKGKLDSV a c c g a a a a t g c c g a t g a a a a c g c g c t a c a g t c t g a c g c t a a a g g c a a a c t t g a t t c t g t c t g g c t t t t a c g g c t a c t t t t g c g c g a t g t c a g a c t g c g a t t t c c g t t t g a a c t a a g a c a g

BspDIClaI 3480 ATDYGAAIDGFIGDVSGLAN g c t a c t g a t t a c g g t g c t g c t a t c g a t g g t t t c a t t g g t g a c g t t t c c g g c c t t g c t a a t c g a t g a c t a a t g c c a c g a c g a t a g c t a c c a a a g t a a c c a c t g c a a a g g c c g g a a c g a t t a

3540 GNGATGDFAGSNSQMAQVGD g g t a a t g g t g c t a c t g g t g a t t t t g c t g g c t c t a a t t c c c a a a t g g c t c a a g t c g g t g a c c c a t t a c c a c g a t g a c c a c t a a a a c g a c c g a g a t t a a g g g t t t a c c g a g t t c a g c c a c t g

3600 GDNSPLMNNFRQYLPSLPQS g g t g a t a a t t c a c c t t t a a t g a a t a a t t t c c g t c a a t a t t t a c c t t c t t t g c c t c a g t c g c c a c t a t t a a g t g g a a a t t a c t t a t t a a a g g c a g t t a t a a a t g g a a g a a a c g g a g t c a g c

NdeI 3660 VECRPYVFGAGKPYEFSIDC g t t g a a t g t c g c c c t t a t g t c t t t g g c g c t g g t a a a c c a t a t g a a t t t t c t a t t g a t t g t c a a c t t a c a g c g g g a a t a c a g a a a c c g c g a c c a t t t g g t a t a c t t a a a a g a t a a c t a a c a

3720 DKINLFRGVFAFLLYVATFM g a c a a a a t a a a c t t a t t c c g t g g t g t c t t t g c g t t t c t t t t a t a t g t t g c c a c c t t t a t g c t g t t t t a t t t g a a t a a g g c a c c a c a g a a a c g c a a a g a a a a t a t a c a a c g g t g g a a a t a c

BmtINheIAflII 3780 YVFSTFANILRNKES* t a t g t a t t t t c g a c g t t t g c t a a c a t a c t g c g t a a t a a g g a g t c t t a a g c t a g c t a a c a g a t a c a t a a a a g c t g c a a a c g a t t g t a t g a c g c a t t a t t c c t c a g a a t t c g a t c g a t t g t c

3840 t c c t a t g a a t c a a c t a c t t a g a t g g t a t t a g t g a c c t g t a a c a g a g c a t t a g c g c a a g g t a g g a t a c t t a g t t g a t g a a t c t a c c a t a a t c a c t g g a c a t t g t c t c g t a a t c g c g t t c c a

BbsI 3900 g a t t t t t g t c t t c t t g c g c t a a t t t t t t g t c a t c a a a c c t g t c g c a c t c c t t a a t a t t t t c t a a a a a c a g a a g a a c g c g a t t a a a a a a c a g t a g t t t g g a c a g c g t g a g g a a t t a t a a a a

3960 f1 ori g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a

PsiI 4020 f1 ori G G T C A F1ori-F c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a

4080 f1 ori A A C C T T G T T C T C A G G T G F1ori-F t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a

DraIIIBsaAIBtgZI 4140 f1 ori c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c

BanII 4200 f1 ori g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c

4260 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g

4320 f1 ori g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g

4340 f1 ori g c t a c a g g g c g c g t c a g g t g c g a t g t c c c g c g c a g t c c a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.