Addgene TFORF1717 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: TFORF1717 Sequencing Result


Map View

T3 (9450 .. 9470) M13 rev M13 Reverse (9415 .. 9431) lac operator M13/pUC Reverse (9396 .. 9418) CAP binding site L4440 (9183 .. 9200) pBR322ori-F (8930 .. 8949) AhdI (8197) ScaI (7716) Amp-R (7628 .. 7647) F1ori-F (7121 .. 7142) F1ori-R (6911 .. 6930) M13/pUC Forward (6675 .. 6697) M13 Forward (6666 .. 6683) M13 fwd T7 (6637 .. 6656) T7 promoter EBV-rev (6412 .. 6431) SV40pA-R (6358 .. 6377) SnaBI (6247) WPRE-R (5403 .. 5423) MluI (5335) SpeI (5249) T3 promoter 5' LTR (truncated) HIV-1 Ψ NruI * (611) NotI (922) KflI - PpuMI (1708) Puro-R (2020 .. 2039) PflFI - Tth111I (2062) BsiWI (2076) BspEI * (2133) RsrII (2136) MscI * (2395) Puro-F (2516 .. 2536) HpaI (2620) EcoRI (2624) cPPT/CTS PaqCI (2826) SbfI (2837) AgeI (2998) FseI (3624) EF1a-F (4046 .. 4066) NdeI (4192) TFORF1717 9493 bp

Sequence View

60 RSV promoter t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t

120 RSV promoter c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g

180 RSV promoter g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t

240 RSV promoter 5' LTR (truncated) t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g

300 5' LTR (truncated) t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c

360 5' LTR (truncated) c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a

420 5' LTR (truncated) g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g

480 HIV-1 Ψ c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g

540 HIV-1 Ψ t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a

600 HIV-1 Ψ t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t

NruI* 660 g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a

720 t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a

780 t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c

840 g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t

900 g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t

NotI 960 MR g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c

1020 DNWRSELYKYKVVKIEPLGV g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t

1080 RRE APTKAKRRVVQREKRAVGIG g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t

1140 RRE ALFLGFLGAAGSTMGAASMT g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c

1200 RRE LTVQARQLLSGIVQQQNNLL c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c

1260 RRE RAIEAQQHLLQLTVWGIKQL a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g

1320 RRE QARILAVERYLKDQQLLGIW c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c

1380 GCSGKLICTTAVPWNASWSN g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a

1440 KSLEQIWNHTTWMEWDREIN a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g

1500 1 KN gp41 peptide NYTSLIHSLIEESQNQQEKN a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a

1560 5 10 15 EQELLELDKWASL (in frame with gp41 peptide) WNWFNIT gp41 peptide EQELLELDKWASLWNWFNIT g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t

1620 (in frame with gp41 peptide) NWLWYIKLFIMIVGGLVGLR NWLWYIKLFIMIVGGLVGLR a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t

1680 (in frame with gp41 peptide) IVFAVLSIVNRVRQGYSPLS IVFAVLSIVNRVRQGYSPLS a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c

PpuMIKflI 1740 (in frame with gp41 peptide) FQTHLPTPRGPMHCISISQQ FQTHLPTPRGPMHCISISQQ t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c a t g c a t t g c a t c t c a a t t a g t c a g c a a a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g t a c g t a a c g t a g a g t t a a t c a g t c g t t

1800 (in frame with gp41 peptide) PGVESPQAPQQAEVCKACVS SV40 promoter SV40 promoter PGVESPQAPQQAEVCKACVS c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c t c a g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g c a g a g t

1860 (in frame with gp41 peptide) ISQQP* SV40 ori SV40 promoter SV40 promoter ISQQP* a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

1920 SV40 promoter SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

1980 SV40 promoter SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

2040 1 5 MTEYKPT SV40 promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPT t t t g c a a a a a g c t t t c t a g a g g t a c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g a a a c g t t t t t c g a a a g a t c t c c a t g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c

BsiWITth111IPflFI 2100 10 15 20 25 VRLATRDDVPRAVRTLAAAF PuroR VRLATRDDVPRAVRTLAAAF g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g

BspEI*RsrII 2160 30 35 40 45 ADYPATRHTVDPDRHIERVT PuroR ADYPATRHTVDPDRHIERVT g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g

2220 50 55 60 65 ELQELFLTRVGLDIGKVWVA PuroR ELQELFLTRVGLDIGKVWVA g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c

2280 70 75 80 85 DDGAAVAVWTTPESVEAGAV PuroR DDGAAVAVWTTPESVEAGAV g a c g a c g g c g c c g c c g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g c t g c t g c c g c g g c g g c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c

2340 90 95 100 105 FAEIGPRMAELSGSRLAAQQ PuroR FAEIGPRMAELSGSRLAAQQ t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t

MscI* 2400 110 115 120 125 QMEGLLAPHRPKEPAWFLAT PuroR QMEGLLAPHRPKEPAWFLAT c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g

2460 130 135 140 145 VGVSPDHQGKGLGSAVVLPG PuroR VGVSPDHQGKGLGSAVVLPG g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t

2520 150 155 160 165 VEAAERAGVPAFLETSAPRN PuroR G C A A C Puro-F VEAAERAGVPAFLETSAPRN g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g

2580 170 175 180 185 LPFYERLGFTVTADVEVPEG PuroR C T C C C C T T C T A C G A G C Puro-F LPFYERLGFTVTADVEVPEG c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t

EcoRIHpaI 2640 190 195 200 PRTWCMTRKPGAS* PuroR PRTWCMTRKPGAS* c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c a g t t a a c g a a t t c t a g a t c t t g a g g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g t c a a t t g c t t a a g a t c t a g a a c t c

2700 cPPT/CTS a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g

2760 cPPT/CTS a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t

2820 cPPT/CTS a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t g g c g t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a a c c g c

SbfIPaqCI 2880 c c g g c t c g a g g c c t g c a g g t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t c t t t g c g g c c g a g c t c c g g a c g t c c a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t a g a a a c g

2940 EF-1 α promoter a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t g c c c g t c a g t g g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a c g g g c a g t c a c c

AgeI 3000 EF-1 α promoter g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t t g a a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a a c t t g g

3060 EF-1 α promoter g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g c t c c g c c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c g a g g c g

3120 EF-1 α promoter c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g t t c t t g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g c a a g a a

3180 EF-1 α promoter EF-1 α intron A t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c g g g c c t a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g c c c g g a

3240 EF-1 α promoter EF-1 α intron A *KWRAATR g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c a g t a c c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a c g t c a t g

3300 EF-1 α promoter EF-1 α intron A SEQDRAEPQFHTPSNSAKRK g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c t t g c g c t c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g a a c g c g a

3360 EF-1 α promoter EF-1 α intron A LLGKAEHKLQPRAQASPGGR t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g

3420 EF-1 α promoter EF-1 α intron A AFRTAGERRDRQKRYTELWK g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a

3480 EF-1 α promoter EF-1 α intron A FNKIVQQSAKKRALYDQLHP t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c

3540 EF-1 α promoter EF-1 α intron A GLDACQYKPKQPRPRRRPGH g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a

3600 EF-1 α promoter EF-1 α intron A TGACMNPSAPGALAAVSFRV g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t

FseI 3660 EF-1 α promoter EF-1 α intron A PTTELQGAQEPAQGRAATYR c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g

3720 EF-1 α promoter EF-1 α intron A GARPPLAPGTPVLQTLPFIA g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c

3780 EF-1 α promoter EF-1 α intron A AERGQQLSSLISSAASPLAP c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c

3840 EF-1 α promoter EF-1 α intron A PHTVWVFSFPRETRLRRKM g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a

3900 EF-1 α promoter EF-1 α intron A g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t t t g g a g t c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a a a c c t c a

3960 EF-1 α promoter EF-1 α intron A a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c

4020 EF-1 α promoter EF-1 α intron A g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a

4080 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F *AESLPEFNKEE t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a

4140 EF-1 α promoter EF-1 α intron A MLDRDVGPT MEPTTLSAGGGHQIPIHAWS c c a t t t c a g g t g t c g t g a g g c t a g c a c c g c c a c c a t g c t g g a t a g g g a t g t g g g c c c a a c g g t a a a g t c c a c a g c a c t c c g a t c g t g g c g g t g g t a c g a c c t a t c c c t a c a c c c g g g t t g

NdeI 4200 PMYPPTYLEPGIGRHTPYGN GHIRRCVQLWPNPPVCWIAV t c c c a t g t a t c c g c c t a c a t a c c t g g a g c c a g g g a t t g g g a g g c a c a c a c c a t a t g g c a a a g g g t a c a t a g g c g g a t g t a t g g a c c t c g g t c c c t a a c c c t c c g t g t g t g g t a t a c c g t t

4260 QTDYRIFELNKRLQNWTEEC LSVVSYKLKVFPKLVPCLLT c c a a a c t g a c t a c a g a a t a t t t g a g c t t a a c a a a c g g c t t c a g a a c t g g a c a g a g g a g t g g g t t t g a c t g a t g t c t t a t a a a c t c g a a t t g t t t g c c g a a g t c t t g a c c t g t c t c c t c a c

4320 DNLWWDAFTTEFFEDDAMLT VIEPPICERSLEKLIIGHQG t g a c a a t c t c t g g t g g g a t g c a t t c a c g a c t g a g t t c t t t g a g g a t g a t g c c a t g t t g a c a c t g t t a g a g a c c a c c c t a c g t a a g t g c t g a c t c a a g a a a c t c c t a c t a c g g t a c a a c t g

4380 ITFCLEDGPKRYTIGRTLIP DSEAQLISWLSIGNAPGQDW c a t c a c t t t c t g c c t g g a g g a t g g a c c a a a g a g a t a t a c c a t t g g c c g g a c c c t g a t c c c g t a g t g a a a g a c g g a c c t c c t a c c t g g t t t c t c t a t a t g g t a a c c g g c c t g g g a c t a g g g

4440 RYFRSIFEGGATELYYVLKH AVEAADKLPTSRLQVINKLV a c g c t a c t t c c g c a g c a t c t t t g a g g g g g g t g c t a c g g a g c t g t a c t a t g t t c t t a a g c a t g c g a t g a a g g c g t c g t a g a a a c t c c c c c c a c g a t g c c t c g a c a t g a t a c a a g a a t t c g t

4500 PKEAFHSNFVSLDCDQGSMV GLLCEVAVKHGEVTVLAAHH c c c c a a g g a g g c a t t c c a c a g c a a c t t t g t g t c c c t c g a c t g t g a c c a g g g c a g c a t g g t g g g g t t c c t c c g t a a g g t g t c g t t g a a a c a c a g g g a g c t g a c a c t g g t c c c g t c g t a c c a

4560 TQHGKPMFTQVCVEGRLYLE GLMALGHEGLHTHLAPQVQL g a c c c a g c a t g g c a a g c c c a t g t t c a c c c a g g t g t g t g t g g a g g g c c g g t t g t a c c t g g a c t g g g t c g t a c c g t t c g g g t a c a a g t g g g t c c a c a c a c a c c t c c c g g c c a a c a t g g a c c t

4620 FMFDDMMRIKTWHFSIRQHR EHKVVHHPYLRPVEADPLVS g t t c a t g t t t g a c g a c a t g a t g c g g a t a a a g a c g t g g c a c t t c a g c a t c c g g c a g c a c c g c a a g t a c a a a c t g c t g t a c t a c g c c t a t t t c t g c a c c g t g a a g t c g t a g g c c g t c g t g g c

4680 ELIPRSILAMHAQDPQMLDQ LEDGAADKGHM a g a g c t c a t c c c c c g c a g c a t c c t t g c c a t g c a t g c c c a a g a c c c c c a g a t g t t g g a t c a t c t c g a g t a g g g g g c g t c g t a g g a a c g g t a c g t a c g g g t t c t g g g g g t c t a c a a c c t a g t

4740 LSKNITRCGLSNSTLNYLRL g c t c t c c a a a a a c a t c a c t c g g t g t g g g c t g t c c a a t t c c a c t c t c a a c t a c c t c c g a c t c g a g a g g t t t t t g t a g t g a g c c a c a c c c g a c a g g t t a a g g t g a g a g t t g a t g g a g g c t g a

4800 CVILEPMQELMSRHKTYSLS c t g t g t g a t a c t c g a g c c c a t g c a a g a g c t c a t g t c a c g c c a c a a g a c c t a c a g c c t c a g g a c a c a c t a t g a g c t c g g g t a c g t t c t c g a g t a c a g t g c g g t g t t c t g g a t g t c g g a g t c

4860 PRDCLKTCLFQKWQRMVAPP c c c c c g c g a c t g c c t c a a g a c c t g c c t t t t c c a g a a g t g g c a g c g c a t g g t a g c a c c c c c g g g g g c g c t g a c g g a g t t c t g g a c g g a a a a g g t c t t c a c c g t c g c g t a c c a t c g t g g g g g

4920 AEPTRQQPSKRRKRKMSGGS t g c g g a g c c c a c a c g t c a g c a g c c c a g c a a a c g g c g g a a a c g g a a g a t g t c a g g g g g c a g a c g c c t c g g g t g t g c a g t c g t c g g g t c g t t t g c c g c c t t t g c c t t c t a c a g t c c c c c g t c

4980 TMSSGGGNTNNSNSKKKSPA c a c c a t g a g c t c t g g t g g t g g c a a c a c c a a c a a c a g c a a c a g c a a g a a g a a g a g c c c a g c g t g g t a c t c g a g a c c a c c a c c g t t g t g g t t g t t g t c g t t g t c g t t c t t c t t c t c g g g t c g

5040 STFALSSQVPDVMVVGEPTL t a g c a c c t t c g c c c t c t c c a g c c a g g t a c c t g a t g t g a t g g t g g t g g g g g a g c c c a c c c t a t c g t g g a a g c g g g a g a g g t c g g t c c a t g g a c t a c a c t a c c a c c a c c c c c t c g g g t g g g a

5100 MGGEFGDEDERLITRLENTQ g a t g g g c g g g g a g t t c g g g g a c g a g g a c g a g a g g c t c a t c a c c c g g c t g g a g a a c a c c c a c t a c c c g c c c c t c a a g c c c c t g c t c c t g c t c t c c g a g t a g t g g g c c g a c c t c t t g t g g g t

5160 FDAANGIDDEDSFNNSPALG g t t t g a c g c a g c c a a c g g c a t t g a c g a c g a g g a c a g c t t t a a c a a c t c c c c t g c a c t g g g c a a a c t g c g t c g g t t g c c g t a a c t g c t g c t c c t g t c g a a a t t g t t g a g g g g a c g t g a c c c

5220 ANSPWNSKPPSSQESKSENP c g c c a a c a g c c c c t g g a a c a g c a a g c c t c c g t c c a g c c a a g a a a g c a a a t c g g a g a a c c c g c g g t t g t c g g g g a c c t t g t c g t t c g g a g g c a g g t c g g t t c t t t c g t t t a g c c t c t t g g g

SpeI 5280 TSQASQ* c a c g t c a c a g g c c t c c c a g t a g t a a t g a a c t a g t c c g t a a c t t g a a a g t a t t t c g a t t t c g t g c a g t g t c c g g a g g g t c a t c a t t a c t t g a t c a g g c a t t g a a c t t t c a t a a a g c t a a a g

MluI 5340 t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a t t g a t c g a a g c g c a g c c t c t a g g a c g c g t a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t a a c t a g c t t c g c g t c g g a g a t c c t g c g c a

5400 WPRE t a a g t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c a t t c a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g

5460 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R MPLYHAI MLL t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a

5520 WPRE ASRMAFIFSSLYKSWLLSLY LPVWLSFSPPCINPGCCLFM g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a

5580 WPRE EELWPVVRQRGVVCTVFADA RSCGPLSGNVAWCALCLLTQ g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t

5640 WPRE TPTGWGIATTCQLLSGTFAF PPLVGALPPPVSSFPGLSLS a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g

5700 WPRE PLPIATAELIAACLARCWTG PSLLPRRNSSPPALPAAGQG c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c

5760 WPRE *RGKR ARLLGTDNSVVLSGKSSSFP LGCWALTIPWCCRGNHRPFL g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a

5820 WPRE (in frame with Factor Xa site) PQEGTNGGPNQAPRGEAVDR WLLACVATWILRGTSFCYVP GCSPVLPPGFCAGRPSATSL t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a

5880 WPRE 1 RGEI Factor Xa site SALNPADLPSRGLLPALRPL RPSIQRTFLPAACCRLCGLF t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a

5940 WPRE PRLRLRPQTSRISLWAASPR RVFAFALRRVGSPFGPPPRV c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c g t g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g c a

6000 RL* DFKTNDLQGSCRS* c g a c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a g c t g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t

6060 3' LTR ( Δ U3) a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t

6120 3' LTR ( Δ U3) c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g

6180 3' LTR ( Δ U3) c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a

6240 3' LTR ( Δ U3) t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t

SnaBI 6300 3' LTR ( Δ U3) g c a g t a c g t a t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t c g t c a t g c a t a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a

6360 SV40 poly(A) signal C G T SV40pA-R g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t

6420 SV40 poly(A) signal G T G G T T T G T EBV-rev T A T C G T A G T G T T T A A A G SV40pA-R a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a

6480 SV40 poly(A) signal C C A A A C T C A T C EBV-rev c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g c c c c t a a c t c c g c c g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g c g g g g a t t g a g g c g g

6540 SV40 ori c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a

6600 SV40 ori t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c

6660 SV40 ori T7 promoter G G G A T A T C A C T C A G C A T A A T T7 a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c

6720 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a

6780 a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g

6840 f1 ori g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g

6900 f1 ori g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g

6960 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g

7020 f1 ori c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g

7080 f1 ori g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c

7140 f1 ori G T G G A C T C T T G T T C C A A A C T F1ori-F g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a

7200 f1 ori G G F1ori-F g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a

7260 f1 ori t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t

7320 f1 ori AmpR promoter a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a

7380 AmpR promoter g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t

7440 1 5 10 signal sequence MSIQHFRVAL AmpR promoter AmpR MSIQHFRVAL t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t

7500 15 20 signal sequence IPFFAAFCLPVFA 25 30 HPETLVK AmpR IPFFAAFCLPVFAHPETLVK t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c

7560 35 40 45 50 VKDAEDQLGARVGYIELDLN AmpR VKDAEDQLGARVGYIELDLN t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t

7620 55 60 65 70 SGKILESFRPEERFPMMSTF AmpR SGKILESFRPEERFPMMSTF g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t

7680 75 80 85 90 KVLLCGAVLSRIDAGQEQLG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R KVLLCGAVLSRIDAGQEQLG a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g

ScaI 7740 95 100 105 110 RRIHYSQNDLVEYSPVTEKH AmpR RRIHYSQNDLVEYSPVTEKH g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g

7800 115 120 125 130 LTDGMTVRELCSAAITMSDN AmpR LTDGMTVRELCSAAITMSDN t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t

7860 135 140 145 150 TAANLLLTTIGGPKELTAFL AmpR TAANLLLTTIGGPKELTAFL c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g

7920 155 160 165 170 HNMGDHVTRLDRWEPELNEA AmpR HNMGDHVTRLDRWEPELNEA a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t

7980 175 180 185 190 IPNDERDTTMPVAMATTLRK AmpR IPNDERDTTMPVAMATTLRK t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g

8040 195 200 205 210 LLTGELLTLASRQQLIDWME AmpR LLTGELLTLASRQQLIDWME t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c

8100 215 220 225 230 ADKVAGPLLRSALPAGWFIA AmpR ADKVAGPLLRSALPAGWFIA c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c

8160 235 240 245 250 DKSGAGERGSRGIIAALGPD AmpR DKSGAGERGSRGIIAALGPD a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c

AhdI 8220 255 260 265 270 GKPSRIVVIYTTGSQATMDE AmpR GKPSRIVVIYTTGSQATMDE g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g

8280 275 280 285 RNRQIAEIGASLIKHW* AmpR RNRQIAEIGASLIKHW* g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g

8340 a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a

8400 a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g

8460 ori a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g

8520 ori g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c

8580 ori a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t

8640 ori a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g

8700 ori c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a

8760 ori g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t

8820 ori c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g

8880 ori t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g

8940 ori G G G A A A C G C C T pBR322ori-F c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a

9000 ori G G T A T C T T T pBR322ori-F g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a

9060 ori g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g

9120 t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c

9180 a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g

9240 A G C G A G T C A G T G A G C G A G L4440 g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c

9300 c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t

9360 -35 CAP binding site lac promoter g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a

9420 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A M13 Reverse t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t

9480 M13 rev T3 promoter A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T A A A G G T3 a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g

9493 t g g a g c t g c a a g c a c c t c g a c g t t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.