Addgene pcmv3-STING-C88S-FLAG Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pcmv3-STING-C88S-FLAG Sequencing Result


Map View

SgrDI (7097) pENTR-R (6949 .. 6968) Kan-R (6865 .. 6884) PasI (6650) EcoNI (6612) SspI (6600) pBR322ori-F (5534 .. 5553) AflIII - PciI (5393) L4440 (5283 .. 5300) CAP binding site M13/pUC Reverse (5065 .. 5087) lac operator M13 Reverse (5052 .. 5068) BstZ17I (5014) EBV-rev (4973 .. 4992) SV40pA-R (4919 .. 4938) SacII (4507) RsrII (4135) PshAI (3754) AvrII (3663) StuI (3662) SV40pro-F (3591 .. 3610) BglII (12) pRS-marker (44 .. 63) MfeI (161) SpeI (267) SnaBI (608) CMV-F (787 .. 807) LNCX (833 .. 857) Eco53kI (834) SacI (836) EcoO109I - PpuMI (990) PaeR7I - XhoI (1029) BlpI (1069) AleI (1317) pMT2-F (1338 .. 1357) T7 (1386 .. 1405) HindIII (1415) Acc65I * (1647) KpnI (1651) BssHII (1675) BsrGI (2010) BsgI (2013) XbaI (2585) NotI (2592) PspOMI (2606) ApaI (2610) BGH-rev (2631 .. 2648) F1ori-R (2994 .. 3013) F1ori-F (3204 .. 3225) pBABE 3' (3344 .. 3364) CsiI - SexAI * (3430) pcmv3-STING-C88S-FLAG 7099 bp

Sequence View

BglII 5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

MfeI 180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

240 t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a g t a c a t t t a t a t t g g c t c a t g t c c a a t a t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t c a t g t a a a t a t a a c c g a g t a c a g g t t a t a

SpeI 300 CMV enhancer g a c c g c c a t g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t c t g g c g g t a c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a

360 CMV enhancer t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c

420 CMV enhancer g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t

480 CMV enhancer c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a

540 CMV enhancer t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a c g g t a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t g c c a t

600 CMV enhancer a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a c g g g a c t t t c c t a c t t g g c a g t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t g c c c t g a a a g g a t g a a c c g t c a

SnaBI 660 CMV enhancer CMV promoter a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c a a t g t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g g t t a c

720 CMV promoter g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c

780 CMV promoter g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c c g c c c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g g c g g g

Eco53kISacI 840 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F A G C T C G T T LNCX c g t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t g c a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a

900 T A G T G A A C C G T C A G A T C LNCX t a g t g a a c c g t c a g a t c c t c a c t c t c t t c c g c a t c g c t g t c t g c g a g g g c c a g c t g t t g g a t c a c t t g g c a g t c t a g g a g t g a g a g a a g g c g t a g c g a c a g a c g c t c c c g g t c g a c a a c c

960 g c t c g c g g t t g a g g a c a a a c t c t t c g c g g t c t t t c c a g t a c t c t t g g a t c g g a a a c c c g t c g a g c g c c a a c t c c t g t t t g a g a a g c g c c a g a a a g g t c a t g a g a a c c t a g c c t t t g g g c a

EcoO109IPpuMI 1020 c g g c c t c c g a a c g g t a c t c c g c c a c c g a g g g a c c t g a g c g a g t c c g c a t c g a c c g g a t c g g c c g g a g g c t t g c c a t g a g g c g g t g g c t c c c t g g a c t c g c t c a g g c g t a g c t g g c c t a g c

BlpIPaeR7IXhoI 1080 g a a a a c c t c t c g a g a a a g g c g t c t a a c c a g t c a c a g t c g c a a g g t a g g c t g a g c a c c g t g c t t t t g g a g a g c t c t t t c c g c a g a t t g g t c a g t g t c a g c g t t c c a t c c g a c t c g t g g c a c

1140 g c g g g c g g c a g c g g g t g g c g g t c g g g g t t g t t t c t g g c g g a g g t g c t g c t g a t g a t g t a a c g c c c g c c g t c g c c c a c c g c c a g c c c c a a c a a a g a c c g c c t c c a c g a c g a c t a c t a c a t t

1200 t t a a a g t a g g c g g t c t t g a g a c g g c g g a t g g t c g a g g t g a g g t g t g g c a g g c t t g a g a t c a a t t t c a t c c g c c a g a a c t c t g c c g c c t a c c a g c t c c a c t c c a c a c c g t c c g a a c t c t a g

1260 c a g c t g t t g g g g t g a g t a c t c c c t c t c a a a a g c g g g c a t t a c t t c t g c g c t a a g a t t g t c g t c g a c a a c c c c a c t c a t g a g g g a g a g t t t t c g c c c g t a a t g a a g a c g c g a t t c t a a c a g

AleI 1320 a g t t t c c a a a a a c g a g g a g g a t t t g a t a t t c a c c t g g c c c g a t c t g g c c a t a c a c t t g a g t c a a a g g t t t t t g c t c c t c c t a a a c t a t a a g t g g a c c g g g c t a g a c c g g t a t g t g a a c t c

1380 T T G C C T T T C T C T C C A C A G G T pMT2-F t g a c a a t g a c a t c c a c t t t g c c t t t c t c t c c a c a g g t g t c c a c t c c c a g g t c c a a g t t t a a c t g t t a c t g t a g g t g a a a c g g a a a g a g a g g t g t c c a c a g g t g a g g g t c c a g g t t c a a a t

HindIII 1440 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 MPYSNLH a a c t t t a a t a c g a c t c a c t a t a g g g g c c g c c a c c a a g c t t a t g c c a t a c t c c a a c c t g c a t t g a a a t t a t g c t g a g t g a t a t c c c c g g c g g t g g t t c g a a t a c g g t a t g a g g t t g g a c g t

1500 PAIPRPRGHRSKYVALIFLV t c c a g c c a t c c c a c g g c c c a g a g g t c a c c g c t c c a a a t a t g t a g c c c t c a t c t t t c t g g t a g g t c g g t a g g g t g c c g g g t c t c c a g t g g c g a g g t t t a t a c a t c g g g a g t a g a a a g a c c a

1560 ASLMILWVAKDPPNHTLKYL g g c c a g c c t g a t g a t c c t t t g g g t g g c a a a g g a t c c a c c a a a t c a c a c t c t g a a g t a c c t c c g g t c g g a c t a c t a g g a a a c c c a c c g t t t c c t a g g t g g t t t a g t g t g a g a c t t c a t g g a

1620 ALHLASHELGLLLKNLCCLA a g c a c t t c a c c t a g c c t c g c a c g a a c t t g g a c t a c t g t t g a a a a a c c t c t g c t g t c t g g c t c g t g a a g t g g a t c g g a g c g t g c t t g a a c c t g a t g a c a a c t t t t t g g a g a c g a c a g a c c g

BssHIIAcc65I*KpnI 1680 EELCHVQSRYQGSYWKAVRA t g a a g a g c t g t g c c a t g t c c a g t c c a g g t a c c a g g g c a g c t a c t g g a a g g c t g t g c g c g c a c t t c t c g a c a c g g t a c a g g t c a g g t c c a t g g t c c c g t c g a t g a c c t t c c g a c a c g c g c g

1740 SLGCPIHCMAMILLSSYFYF c a g c c t g g g a t g c c c c a t c c a c t g t a t g g c t a t g a t t c t a c t a t c g t c t t a t t t c t a t t t g t c g g a c c c t a c g g g g t a g g t g a c a t a c c g a t a c t a a g a t g a t a g c a g a a t a a a g a t a a a

1800 LQNTADIYLSWMFGLLVLYK c c t c c a a a a c a c t g c t g a c a t a t a c c t c a g t t g g a t g t t t g g c c t t c t g g t c c t c t a t a a g g a g g t t t t g t g a c g a c t g t a t a t g g a g t c a a c c t a c a a a c c g g a a g a c c a g g a g a t a t t

1860 SLSMLLGLQSLTPAEVSAVC g t c c c t a a g c a t g c t c c t g g g c c t t c a g a g c t t g a c t c c a g c g g a a g t c t c t g c a g t c t g c a g g g a t t c g t a c g a g g a c c c g g a a g t c t c g a a c t g a g g t c g c c t t c a g a g a c g t c a g a c

1920 EEKKLNVAHGLAWSYYIGYL t g a a g a a a a g a a g t t a a a t g t t g c c c a c g g g c t g g c c t g g t c a t a c t a c a t t g g g t a c t t a c t t c t t t t c t t c a a t t t a c a a c g g g t g c c c g a c c g g a c c a g t a t g a t g t a a c c c a t g a a

1980 RLILPGLQARIRMFNQLHNN g c g g t t g a t c t t a c c a g g g c t c c a g g c c c g g a t c c g a a t g t t c a a t c a g c t a c a t a a c a a c g c c a a c t a g a a t g g t c c c g a g g t c c g g g c c t a g g c t t a c a a g t t a g t c g a t g t a t t g t t

BsrGIBsgI 2040 MLSGAGSRRLYILFPLDCGV c a t g c t c a g t g g t g c a g g g a g c c g a a g a c t g t a c a t c c t c t t t c c a t t g g a c t g t g g g g t g t a c g a g t c a c c a c g t c c c t c g g c t t c t g a c a t g t a g g a g a a a g g t a a c c t g a c a c c c c a

2100 PDNLSVVDPNIRFRDMLPQQ g c c t g a c a a c c t g a g t g t a g t t g a c c c c a a c a t t c g a t t c c g a g a t a t g c t g c c c c a g c a c g g a c t g t t g g a c t c a c a t c a a c t g g g g t t g t a a g c t a a g g c t c t a t a c g a c g g g g t c g t

2160 NIDRAGIKNRVYSNSVYEIL a a a c a t c g a c c g t g c t g g c a t c a a g a a t c g g g t t t a t t c c a a c a g c g t c t a c g a g a t t c t t t t g t a g c t g g c a c g a c c g t a g t t c t t a g c c c a a a t a a g g t t g t c g c a g a t g c t c t a a g a

2220 ENGQPAGVCILEYATPLQTL g g a g a a c g g a c a g c c a g c a g g c g t c t g t a t c c t g g a g t a c g c c a c c c c c t t g c a g a c c c t c c t c t t g c c t g t c g g t c g t c c g c a g a c a t a g g a c c t c a t g c g g t g g g g g a a c g t c t g g g a

2280 FAMSQDAKAGFSREDRLEQA g t t t g c c a t g t c a c a g g a t g c c a a a g c t g g c t t c a g t c g g g a g g a t c g g c t t g a g c a g g c c a a a c g g t a c a g t g t c c t a c g g t t t c g a c c g a a g t c a g c c c t c c t a g c c g a a c t c g t c c g

2340 KLFCRTLEEILEDVPESRNN t a a a c t c t t c t g c c g g a c a c t t g a g g a a a t c c t g g a a g a t g t c c c c g a g t c t c g a a a t a a a t t t g a g a a g a c g g c c t g t g a a c t c c t t t a g g a c c t t c t a c a g g g g c t c a g a g c t t t a t t

2400 CRLIVYQEPTDGNSFSLSQE c t g c c g c c t c a t t g t c t a c c a a g a a c c c a c a g a c g g a a a c a g t t t c t c a c t g t c t c a g g a g a c g g c g g a g t a a c a g a t g g t t c t t g g g t g t c t g c c t t t g t c a a a g a g t g a c a g a g t c c t

2460 VLRHIRQEEKEEVTMNAPMT g g t g c t c c g g c a c a t t c g t c a g g a a g a a a a g g a g g a g g t t a c c a t g a a t g c c c c c a t g a c c c a c g a g g c c g t g t a a g c a g t c c t t c t t t t c c t c c t c c a a t g g t a c t t a c g g g g g t a c t g

2520 SVAPPPSVLSQEPRLLISGM c t c a g t g g c a c c t c c t c c c t c c g t a c t g t c c c a a g a g c c a a g a c t c c t c a t c a g t g g t a t g a g t c a c c g t g g a g g a g g g a g g c a t g a c a g g g t t c t c g g t t c t g a g g a g t a g t c a c c a t a

2580 1 5 DYKDDDDK * FLAG DQPLPLRTDLIDYKDDDDK* g g a t c a g c c t c t c c c a c t c c g c a c t g a c c t c a t c g a t t a c a a g g a c g a c g a t g a c a a g t g c c t a g t c g g a g a g g g t g a g g c g t g a c t g g a g t a g c t a a t g t t c c t g c t g c t a c t g t t c a c

PspOMIApaINotIXbaI 2640 bGH poly(A) signal G G A G C T G A C A BGH-rev a t a a t c t a g a g c g g c c g c c g a a t t c g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t t a t t a g a t c t c g c c g g c g g c t t a a g c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a

2700 bGH poly(A) signal C G G A A G A T BGH-rev g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t

2760 bGH poly(A) signal a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c

2820 bGH poly(A) signal t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t

2880 bGH poly(A) signal a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g

2940 f1 ori c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c

3000 f1 ori G A G G A A A F1ori-R t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a

3060 f1 ori G C G A A A G A A G G G A F1ori-R c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c a g g c t t t c c c c g t c a a g c t c t a a a t c g g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g t c c g a a a g g g g c a g t t c g a g a t t t a g c

3120 f1 ori g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t

3180 f1 ori t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g

3240 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g

3300 f1 ori t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t

3360 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T pBABE 3' a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t

3420 SV40 promoter C C C A pBABE 3' g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a

SexAI*CsiI 3480 SV40 promoter t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g

3540 SV40 promoter SV40 ori a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t

3600 SV40 promoter SV40 ori T A T T T A T G C A SV40pro-F a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t

3660 SV40 promoter SV40 ori G A G G C C G A G G SV40pro-F g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t

StuIAvrII 3720 SV40 promoter SV40 ori *AKAFLERSSTYGNESRIL g g c c t a g g c t t t t g c a a a a a g c t c t c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c c g g a t c c g a a a a c g t t t t t c g a g a g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c

PshAI 3780 1 5 10 15 MKKPELTATSVEKFLIEK HygR 1 5 10 15 MKKPELTATSVEKFLIEK HygR MKKPELTATSVEKFLIEK VHHFLRFEGRRRDLLKQDFL c a c g t g a t g a a a a a g c c t g a a c t c a c c g c g a c g t c t g t c g a g a a g t t t c t g a t c g a a a a g g t g c a c t a c t t t t t c g g a c t t g a g t g g c g c t g c a g a c a g c t c t t c a a a g a c t a g c t t t t c

3840 20 25 30 35 FDSVSDLMQLSEGEESRAFS HygR 20 25 30 35 FDSVSDLMQLSEGEESRAFS HygR FDSVSDLMQLSEGEESRAFS EVADGVQHLERLAFFRTSEA t t c g a c a g c g t c t c c g a c c t g a t g c a g c t c t c g g a g g g c g a a g a a t c t c g t g c t t t c a g c a a g c t g t c g c a g a g g c t g g a c t a c g t c g a g a g c c t c c c g c t t c t t a g a g c a c g a a a g t c g

3900 40 45 50 55 FDVGGRGYVLRVNSCADGFY HygR 40 45 50 55 FDVGGRGYVLRVNSCADGFY HygR FDVGGRGYVLRVNSCADGFY EIYSPTSIDQPYIAAGITEV t t c g a t g t a g g a g g g c g t g g a t a t g t c c t g c g g g t a a a t a g c t g c g c c g a t g g t t t c t a c a a g c t a c a t c c t c c c g c a c c t a t a c a g g a c g c c c a t t t a t c g a c g c g g c t a c c a a a g a t g

3960 60 65 70 75 KDRYVYRHFASAALPIPEVL HygR 60 65 70 75 KDRYVYRHFASAALPIPEVL HygR KDRYVYRHFASAALPIPEVL FITINIPVKCRGRERNRFHK a a a g a t c g t t a t g t t t a t c g g c a c t t t g c a t c g g c c g c g c t c c c g a t t c c g g a a g t g c t t t t t c t a g c a a t a c a a a t a g c c g t g a a a c g t a g c c g g c g c g a g g g c t a a g g c c t t c a c g a a

4020 80 85 90 95 DIGEFSESLTYCISRRAQGV HygR 80 85 90 95 DIGEFSESLTYCISRRAQGV HygR DIGEFSESLTYCISRRAQGV VNPFEALAQGIADGATCLTD g a c a t t g g g g a a t t c a g c g a g a g c c t g a c c t a t t g c a t c t c c c g c c g t g c a c a g g g t g t c c t g t a a c c c c t t a a g t c g c t c t c g g a c t g g a t a a c g t a g a g g g c g g c a c g t g t c c c a c a g

4080 100 105 110 115 TLQDLPETELPAVLQPVAEA HygR 100 105 110 115 TLQDLPETELPAVLQPVAEA HygR TLQDLPETELPAVLQPVAEA RQLVQRFGFQGSNQLRDRLG a c g t t g c a a g a c c t g c c t g a a a c c g a a c t g c c c g c t g t t c t g c a g c c g g t c g c g g a g g c c t g c a a c g t t c t g g a c g g a c t t t g g c t t g a c g g g c g a c a a g a c g t c g g c c a g c g c c t c c g g

RsrII 4140 120 125 130 135 MDAIAAADLSQTSGFGPFGP HygR 120 125 130 135 MDAIAAADLSQTSGFGPFGP HygR MDAIAAADLSQTSGFGPFGP HIRDSRGIKALRAPEAWESR a t g g a t g c g a t c g c t g c g g c c g a t c t t a g c c a g a c g a g c g g g t t c g g c c c a t t c g g a c c g t a c c t a c g c t a g c g a c g c c g g c t a g a a t c g g t c t g c t c g c c c a a g c c g g g t a a g c c t g g c

4200 140 145 150 155 QGIGQYTTWRDFICAIADPH HygR 140 145 150 155 QGIGQYTTWRDFICAIADPH HygR QGIGQYTTWRDFICAIADPH LSDTLVSCPTIEYARNSIGM c a a g g a a t c g g t c a a t a c a c t a c a t g g c g t g a t t t c a t a t g c g c g a t t g c t g a t c c c c a t g t t c c t t a g c c a g t t a t g t g a t g t a c c g c a c t a a a g t a t a c g c g c t a a c g a c t a g g g g t a

4260 160 165 170 175 VYHWQTVMDDTVSASVAQAL HygR 160 165 170 175 VYHWQTVMDDTVSASVAQAL HygR VYHWQTVMDDTVSASVAQAL HIVPLSHHVVGDTRGDRLSE g t g t a t c a c t g g c a a a c t g t g a t g g a c g a c a c c g t c a g t g c g t c c g t c g c g c a g g c t c t c c a c a t a g t g a c c g t t t g a c a c t a c c t g c t g t g g c a g t c a c g c a g g c a g c g c g t c c g a g a g

4320 180 185 190 195 DELMLWAEDCPEVRHLVHAD HygR 180 185 190 195 DELMLWAEDCPEVRHLVHAD HygR DELMLWAEDCPEVRHLVHAD ILQHKPGLVAGFDPVEHVRI g a t g a g c t g a t g c t t t g g g c c g a g g a c t g c c c c g a a g t c c g g c a c c t c g t g c a c g c g g a t c t a c t c g a c t a c g a a a c c c g g c t c c t g a c g g g g c t t c a g g c c g t g g a g c a c g t g c g c c t a

4380 200 205 210 215 FGSNNVLTDNGRITAVIDWS HygR 200 205 210 215 FGSNNVLTDNGRITAVIDWS HygR FGSNNVLTDNGRITAVIDWS EAGVIDQRVIAAYCRDNVPA t t c g g c t c c a a c a a t g t c c t g a c g g a c a a t g g c c g c a t a a c a g c g g t c a t t g a c t g g a g c a a g c c g a g g t t g t t a c a g g a c t g c c t g t t a c c g g c g t a t t g t c g c c a g t a a c t g a c c t c g

4440 220 225 230 235 EAMFGDSQYEVANIFFWRPW HygR 220 225 230 235 EAMFGDSQYEVANIFFWRPW HygR EAMFGDSQYEVANIFFWRPW LRHEPIGLVLDGVDEEPPRP g a g g c g a t g t t c g g g g a t t c c c a a t a c g a g g t c g c c a a c a t c t t c t t c t g g a g g c c g t g g c t c c g c t a c a a g c c c c t a a g g g t t a t g c t c c a g c g g t t g t a g a a g a a g a c c t c c g g c a c c

4500 240 245 250 255 LACMEQQTRYFERRHPELAG HygR 240 245 250 255 LACMEQQTRYFERRHPELAG HygR LACMEQQTRYFERRHPELAG QSTHLLLRAVELPPM t t g g c t t g t a t g g a g c a g c a g a c g c g c t a c t t c g a g c g g a g g c a t c c g g a g c t t g c a g g a a a c c g a a c a t a c c t c g t c g t c t g c g c g a t g a a g c t c g c c t c c g t a g g c c t c g a a c g t c c t

SacII 4560 260 265 270 275 SPRLRAYMLRIGLDQLYQSL HygR 260 265 270 275 SPRLRAYMLRIGLDQLYQSL HygR SPRLRAYMLRIGLDQLYQSL t c g c c g c g g c t c c g g g c g t a t a t g c t c c g c a t t g g t c t t g a c c a a c t c t a t c a g a g c t t g a g c g g c g c c g a g g c c c g c a t a t a c g a g g c g t a a c c a g a a c t g g t t g a g a t a g t c t c g a a c

4620 280 285 290 295 VDGNFDDAAWAQGRCDAIVR HygR 280 285 290 295 VDGNFDDAAWAQGRCDAIVR HygR VDGNFDDAAWAQGRCDAIVR g t t g a c g g c a a t t t c g a t g a t g c a g c t t g g g c g c a g g g t c g a t g c g a c g c a a t c g t c c g a c a a c t g c c g t t a a a g c t a c t a c g t c g a a c c c g c g t c c c a g c t a c g c t g c g t t a g c a g g c t

4680 300 305 310 315 SGAGTVGRTQIARRSAAVWT HygR 300 305 310 315 SGAGTVGRTQIARRSAAVWT HygR SGAGTVGRTQIARRSAAVWT t c c g g a g c c g g g a c t g t c g g g c g t a c a c a a a t c g c c c g c a g a a g c g c g g c c g t c t g g a c c a g g c c t c g g c c c t g a c a g c c c g c a t g t g t t t a g c g g g c g t c t t c g c g c c g g c a g a c c t g g

4740 320 325 330 335 DGCVEVLADSGNRRPSTRPR HygR 320 325 330 335 DGCVEVLADSGNRRPSTRPR HygR DGCVEVLADSGNRRPSTRPR g a t g g c t g t g t a g a a g t a c t c g c c g a t a g t g g a a a c c g a c g c c c c a g c a c t c g t c c g a g g c t a c c g a c a c a t c t t c a t g a g c g g c t a t c a c c t t t g g c t g c g g g g t c g t g a g c a g g c t c c

4800 340 AKE* HygR 340 AKE* HygR AKE* g c a a a g g a a t a g c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g c g t t t c c t t a t c g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c

4860 g g c t t c g g a a t c g t t t t c c g g g a c g c t g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c c g a a g c c t t a g c a a a a g g c c c t g c g a c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c

4920 SV40 poly(A) signal C G SV40pA-R c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g

4980 SV40 poly(A) signal G T G G T T T G EBV-rev T T A T C G T A G T G T T T A A A G SV40pA-R a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c

BstZ17I 5040 SV40 poly(A) signal T C C A A A C T C A T C EBV-rev t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c

5100 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g

5160 -10 -35 lac promoter CAP binding site a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g

5220 CAP binding site a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c

5280 c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a

5340 G A G C G A G T G A C T G A G C G A L4440 t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g

AflIIIPciI 5400 t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t

5460 ori g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a

5520 ori a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g

5580 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a

5640 ori g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c

5700 ori c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c

5760 ori g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a

5820 ori c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c

5880 ori a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g

5940 ori g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t

6000 ori a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a

6060 ori g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a

6120 t c t a c g g g g t c t g a g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g a g a t g c c c c a g a c t c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c

6180 270 265 260 *FFEDLMLHFQLK KanR 270 265 260 *FFEDLMLHFQLK KanR *FFEDLMLHFQLK t a t c c g c t c a t g a a t t a a t t c t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t a g g c g a g t a c t t a a t t a a g a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a

6240 255 250 245 240 NMDPNDIGYKQFLRKQLSPS KanR 255 250 245 240 NMDPNDIGYKQFLRKQLSPS KanR NMDPNDIGYKQFLRKQLSPS a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t

6300 235 230 225 220 FEGLCNWLIALDQYRDAIGV KanR 235 230 225 220 FEGLCNWLIALDQYRDAIGV KanR FEGLCNWLIALDQYRDAIGV a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g

6360 215 210 205 200 RGVDICGILKGEDFILNDLS KanR 215 210 205 200 RGVDICGILKGEDFILNDLS KanR RGVDICGILKGEDFILNDLS t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t

6420 195 190 185 180 FDGHTVVSDPSFPLLKHMEK KanR 195 190 185 180 FDGHTVVSDPSFPLLKHMEK KanR FDGHTVVSDPSFPLLKHMEK g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t a t g c a t t t c t t t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a t a c g t a a a g a a a

6480 175 170 165 160 WVQEVPWGNREDDFDSADVL KanR 175 170 165 160 WVQEVPWGNREDDFDSADVL KanR WVQEVPWGNREDDFDSADVL c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t

6540 155 150 145 140 GNNMRSQAQALRFVRDSNFP KanR 155 150 145 140 GNNMRSQAQALRFVRDSNFP KanR GNNMRSQAQALRFVRDSNFP a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c

SspI 6600 135 130 125 120 CNCVPISHLRRLFVALADVI KanR 135 130 125 120 CNCVPISHLRRLFVALADVI KanR CNCVPISHLRRLFVALADVI a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a

PasIEcoNI 6660 115 110 105 100 NEGSDPYEELVQFATKGPIA KanR 115 110 105 100 NEGSDPYEELVQFATKGPIA KanR NEGSDPYEELVQFATKGPIA a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c a g g g a t c g c t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g t c c c t a g c g

6720 95 90 85 80 TTLLWADDPTRIFHKITPLP KanR 95 90 85 80 TTLLWADDPTRIFHKITPLP KanR TTLLWADDPTRIFHKITPLP a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c

6780 75 70 65 60 MFETLWNLRVMEDTVDNAVS KanR 75 70 65 60 MFETLWNLRVMEDTVDNAVS KanR MFETLWNLRVMEDTVDNAVS c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a

6840 55 50 45 40 GKGHKLFLEPADPKGYLRYI KanR 55 50 45 40 GKGHKLFLEPADPKGYLRYI KanR GKGHKLFLEPADPKGYLRYI a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a t c g a t a g a t t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t a g c t a t c t a

6900 35 30 25 20 TAGSQGVNDRAWKYGYLDAD KanR 35 30 25 20 TAGSQGVNDRAWKYGYLDAD KanR A T C G C G A G C C C A T T T A T A C C Kan-R TAGSQGVNDRAWKYGYLDAD t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g

6960 15 10 5 1 MNSNLRPRSCSTERQIHSM KanR 15 10 5 1 MNSNLRPRSCSTERQIHSM KanR A T G G C T C A T A A C pENTR-R MNSNLRPRSCSTERQIHSM c a t g t t g g a a t t t a a t c g c g g c c t a g a g c a a g a c g t t t c c c g t t g a a t a t g g c t c a t a a c g t a c a a c c t t a a a t t a g c g c c g g a t c t c g t t c t g c a a a g g g c a a c t t a t a c c g a g t a t t g

7020 A C C C C T T G pENTR-R a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a c c a a a a t c c c t t t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t g g t t t t a g g g a a

7080 a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a t c c g c g c a c a t t t c c c c g t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t a g g c g c g t g t a a a g g g g c

SgrDI 3ʹ 5ʹ 7099 a a a a g t g c c a c c t g a c g t c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.