Addgene JAB2518 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: JAB2518 Sequencing Result


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PsiI (5506) FseI (5488) BstEII (4407) XmnI (3828) EcoRI (3767) AlwNI (3459) pBR322ori-F (3184 .. 3203) BglII (3038) RB T-DNA repeat StuI (2985) L4440 (2867 .. 2884) TaqII (2879) BspQI - SapI (2861) LB T-DNA repeat MauBI (122) NsiI (234) AflII (317) FspI - FspAI (519) EcoO109I - KflI - PpuMI (696) AfeI (756) PflFI - Tth111I (773) SspI (1020) NheI (1025) BmtI (1029) Bsu36I (1041) AclI (1132) DraI - PmeI (1292) SbfI (1351) EcoRV (1355) 35S promoter (1460 .. 1480) XcmI (1536) BstAPI (2227) SV40 NLS ScaI (2500) SpeI (2549) NotI (2562) AleI (2573) T3 (2597 .. 2617) M13 rev M13 Reverse (2636 .. 2652) lac operator M13/pUC Reverse (2649 .. 2671) CAP binding site JAB2518 5651 bp

Sequence View

60 LB T-DNA repeat c t g g t g g c a g g a t a t a t t g t g g t g t a a a c a a a t t g a c g c t t a g a c a a c t t a a t a a c a c a t g a c c a c c g t c c t a t a t a a c a c c a c a t t t g t t t a a c t g c g a a t c t g t t g a a t t a t t g t g t a

120 NOS terminator t g c g g a c g t t t t t a a t g t a a t c a g c t t g c a t g c c g g t c g a t c t a g t a a c a t a g a t g a c a c a c g c c t g c a a a a a t t a c a t t a g t c g a a c g t a c g g c c a g c t a g a t c a t t g t a t c t a c t g t g

MauBI 180 NOS terminator c g c g c g c g a t a a t t t a t c c t a g t t t g c g c g c t a t a t t t t g t t t t c t a t c g c g t a t t a a a t g c g c g c g c t a t t a a a t a g g a t c a a a c g c g c g a t a t a a a a c a a a a g a t a g c g c a t a a t t t a

NsiI 240 NOS terminator g t a t a a t t g c g g g a c t c t a a t c a t a a a a a c c c a t c t c a t a a a t a a c g t c a t g c a t t a c a t c a t a t t a a c g c c c t g a g a t t a g t a t t t t t g g g t a g a g t a t t t a t t g c a g t a c g t a a t g t a

300 NOS terminator g t t a a t t a t t a c a t g c t t a a c g t a a t t c a a c a g a a a t t a t a t g a t a a t c a t c g c a a g a c c c a a t t a a t a a t g t a c g a a t t g c a t t a a g t t g t c t t t a a t a t a c t a t t a g t a g c g t t c t g g

AflII 360 NOS terminator g g c a a c a g g a t t c a a t c t t a a g a a a c t t t a t t g c c a a a t g t t t g a a c g a t c t g c t t g a c t c c g t t g t c c t a a g t t a g a a t t c t t t g a a a t a a c g g t t t a c a a a c t t g c t a g a c g a a c t g a

420 180 175 170 *IETVPLVPRPPVPLSF BlpR *IETVPLVPRPPVPLSF c t a g g g g t c a t c a g a t t t c g g t g a c g g g c a g g a c c g g a c g g g g c g g c a c c g g c a g g c t g a g a t c c c c a g t a g t c t a a a g c c a c t g c c c g t c c t g g c c t g c c c c g c c g t g g c c g t c c g a c t

480 165 160 155 150 DLQWFGVDHWNGHKFGAARL BlpR MPVPVLEAGRPQ DLQWFGVDHWNGHKFGAARL a g t c c a g c t g c c a g a a a c c c a c g t c a t g c c a g t t c c c g t g c t t g a a g c c g g c c g c c c g c a t c a g g t c g a c g g t c t t t g g g t g c a g t a c g g t c a a g g g c a c g a a c t t c g g c c g g c g g g c g t

FspAIFspI 540 145 140 135 130 MGRPAYGLAEHMRVSPDNPL BlpR HAAGGISERLVHAHARVVGQ MGRPAYGLAEHMRVSPDNPL g c a t g c c g c g g g g g g c a t a t c c g a g c g c c t c g t g c a t g c g c a c g c t c g g g t c g t t g g g c a c g t a c g g c g c c c c c c g t a t a g g c t c g c g g a g c a c g t a c g c g t g c g a g c c c a g c a a c c c g t

600 125 120 115 110 GIVAVVSKFGQAELSKLLHT BlpR PDDSDHALEALCLQGLQQVG GIVAVVSKFGQAELSKLLHT g c c c g a t g a c a g c g a c c a c g c t c t t g a a g c c c t g t g c c t c c a g g g a c t t c a g c a g g t g g g c g g g c t a c t g t c g c t g g t g c g a g a a c t t c g g g a c a c g g a g g t c c c t g a a g t c g t c c a c c c

660 105 100 95 90 YLTSGLGTRQHRPSVYVTSE BlpR VERGAQSRPLVAGGHVHGRL YLTSGLGTRQHRPSVYVTSE t g t a g a g c g t g g a g c c c a g t c c c g t c c g c t g g t g g c g g g g g g a c a c g t a c a c g g t c g a c t a c a t c t c g c a c c t c g g g t c a g g g c a g g c g a c c a c c g c c c c c c t g t g c a t g t g c c a g c t g a

EcoO109IPpuMIKflI 720 85 80 75 70 ATWDYANRAKWPGAYAIGAV BlpR GRPVVGVACLPGTRVGDAGD ATWDYANRAKWPGAYAIGAV c g g c c g t c c a g t c g t a g g c g t t g c g t g c c t t c c a g g g a c c c g c g t a g g c g a t g c c g g c g a g c c g g c a g g t c a g c a t c c g c a a c g c a c g g a a g g t c c c t g g g c g c a t c c g c t a c g g c c g c t

Tth111IPflFIAfeI 780 65 60 55 50 EGDVEAVLWPYRERLRVLDD BlpR LAVHLGDEPGIALPQTDEVV EGDVEAVLWPYRERLRVLDD c c t c g c c g t c c a c c t c g g c g a c g a g c c a g g g a t a g c g c t c c c g c a g a c g g a c g a g g t c g t g g a g c g g c a g g t g g a g c c g c t g c t c g g t c c c t a t c g c g a g g g c g t c t g c c t g c t c c a g c a

840 45 40 35 30 TWEQPEQPETRFNVTSTEIY BlpR RPLLRFLRLGTEVDRACLDV TWEQPEQPETRFNVTSTEIY c c g t c c a c t c c t g c g g t t c c t g c g g c t c g g t a c g g a a g t t g a c c g t g c t t g t c t c g a t g t g g c a g g t g a g g a c g c c a a g g a c g c c g a g c c a t g c c t t c a a c t g g c a c g a a c a g a g c t a c a

900 25 20 15 10 HNVITCVAPMDAETARRIDA BlpR VVDDGADRRHVRLGGTADVG HNVITCVAPMDAETARRIDA a g t g g t t g a c g a t g g t g c a g a c c g c c g g c a t g t c c g c c t c g g t g g c a c g g c g g a t g t c g g t c a c c a a c t g c t a c c a c g t c t g g c g g c c g t a c a g g c g g a g c c a c c g t g c c g c c t a c a g c c

960 5 1 PRREPSM BlpR RASFWAHGRSPRSS* PRREPSM c c g g g c g t c g t t c t g g g c t c a t g g t a g a t c c c c t c g a t c g a g t t g a g a g t g a a t a t g a g a g g c c c g c a g c a a g a c c c g a g t a c c a t c t a g g g g a g c t a g c t c a a c t c t c a c t t a t a c t c t

SspI 1020 NOS promoter c t c t a a t t g g a t a c c g a g g g g a a t t t a t g g a a c g t c a g t g g a g c a t t t t t g a c a a g a a a t g a g a t t a a c c t a t g g c t c c c c t t a a a t a c c t t g c a g t c a c c t c g t a a a a a c t g t t c t t t a

Bsu36IBmtINheI 1080 NOS promoter a t t t g c t a g c t g a t a g t g a c c t t a g g c g a c t t t t g a a c g c g c a a t a a t g g t t t c t g a c g t t a a a c g a t c g a c t a t c a c t g g a a t c c g c t g a a a a c t t g c g c g t t a t t a c c a a a g a c t g c a

AclI 1140 NOS promoter a t g t g c t t a g c t c a t t a a a c t c c a g a a a c c c g c g g c t c a g t g g c t c c t t c a a c g t t g c g g t a c a c g a a t c g a g t a a t t t g a g g t c t t t g g g c g c c g a g t c a c c g a g g a a g t t g c a a c g c c

1200 NOS promoter t t c t g t c a g t t c c a a a c g t a a a a c g g c t t g t c c c g c g t c a t c g g c g g g g g t c a t a a c g t g a a g a c a g t c a a g g t t t g c a t t t t g c c g a a c a g g g c g c a g t a g c c g c c c c c a g t a t t g c a c

1260 a c t c c c t t a a t t c t c c g c t c a t g a t c g a t a a c a t g t c g a c t a t c g g a c g a g c t c a a a g t t t g a g g g a a t t a a g a g g c g a g t a c t a g c t a t t g t a c a g c t g a t a g c c t g c t c g a g t t t c a a

PmeIDraI 1320 t a a c g t g t g a a g a c g t g c t t c t a a t g t a g t t t a a a c t g a a g g c g g g a a a c g a c a a t c t g a a t t g c a c a c t t c t g c a c g a a g a t t a c a t c a a a t t t g a c t t c c g c c c t t t g c t g t t a g a c t

EcoRVSbfI 1380 t c c a a g c t c a a g c t a a g c t t g c a t g c c t g c a g g a t a t c g t g g a t c c a a g c t t g c c a c g t g a g g t t c g a g t t c g a t t c g a a c g t a c g g a c g t c c t a t a g c a c c t a g g t t c g a a c g g t g c a c

1440 c c g c c a c g t g c c g c c a c g t g c c g c c a c g t g c c t c t a g a g g a t c c a t c t c c a c t g a c g t a a g g c g g t g c a c g g c g g t g c a c g g c g g t g c a c g g a g a t c t c c t a g g t a g a g g t g a c t g c a t t

1500 minimal CaMV 35S promoter C T A T C C T T C G C A A G A C C C T T C 35S promoter MTHNPTILRKTLPLYKEVH *ILFNM g g g a t g a c g c a c a a t c c c a c t a t c c t t c g c a a g a c c c t t c c t c t a t a t a a g g a a g t t c a t c c c t a c t g c g t g t t a g g g t g a t a g g a a g c g t t c t g g g a a g g a g a t a t a t t c c t t c a a g t a

XcmI 1560 minimal CaMV 35S promoter FIWRGHAGIPTMIVGEDQLW ENPSSVSPDGGHNHTFILQP t t c a t t t g g a g a g g a c a c g c t g g g a t c c c c a c c a t g a t t g t g g g t g a a g a t c a g c t g t g g a a g t a a a c c t c t c c t g t g c g a c c c t a g g g g t g g t a c t a a c a c c c a c t t c t a g t c g a c a c c

1620 GRRTLEFVDSVERLEAPALI TTTCQFKNIANFTQFCRCQN g g t c g t c g t a c a c t g g a a t t t g t t g a t a g c g t t g a a c g t c t g g a a g c a c c g g c a c t g a t t c c a g c a g c a t g t g a c c t t a a a c a a c t a t c g c a a c t t g c a g a c c t t c g t g g c c g t g a c t a a

1680 SRFESLIASCGFTAYIMAGL ATKFAQNCATTKGGIDHCTQ a g c c g t t t t g a a a g c c t g a t t g c a a g c t g t g g t t t t a c c g c c t a t a t c a t g g c a g g t c t g t c g g c a a a a c t t t c g g a c t a a c g t t c g a c a c c a a a a t g g c g g a t a t a g t a c c g t c c a g a c

1740 PSRNAGLPELTLANGWPRDW RATIGTQRFQGQCITPRTIP c c g a g c c g t a a t g c c g g t c t g c c g g a a c t g a c c c t g g c a a a t g g t t g g c c t c g t g a t t g g g g c t c g g c a t t a c g g c c a g a c g g c c t t g a c t g g g a c c g t t t a c c a a c c g g a g c a c t a a c c

1800 FDLYVSENFSAVDPVPRYGA KIQINAFVKACNIRNRTITC t t t g a t c t g t a t g t t a g c g a a a a c t t t a g c g c a g t t g a t c c g g t t c c g c g t t a t g g t g c a a a a c t a g a c a t a c a a t c g c t t t t g a a a t c g c g t c a a c t a g g c c a a g g c g c a a t a c c a c g t

1860 TTVHPFVWSDAPYDRDRDQA GSNMRKNPTICRIITVTILC a c c a c t g t t c a t c c g t t t g t t t g g a g t g a t g c a c c g t a t g a t c g t g a c c g t g a t c a g g c a t g g t g a c a a g t a g g c a a a c a a a c c t c a c t a c g t g g c a t a c t a g c a c t g g c a c t a g t c c g t

1920 AHRVMTRAAEFGLVEGYCIP CMTNHGTCCFKTQNFTITNR g c a c a t c g t g t t a t g a c c c g t g c a g c a g a a t t t g g t c t g g t t g a a g g t t a t t g t a t t c c g c g t g t a g c a c a a t a c t g g g c a c g t c g t c t t a a a c c a g a c c a a c t t c c a a t a a c a t a a g g c

1980 LHYDDGSAAISMAGEDPDLS QMVIITACCNTHCTFIRIQT c t g c a t t a c g a t g a t g g t a g c g c a g c a a t t a g t a t g g c a g g t g a a g a t c c t g a t c t g a g t g a c g t a a t g c t a c t a c c a t c g c g t c g t t a a t c a t a c c g t c c a c t t c t a g g a c t a g a c t c a

2040 PAARGVMQLVSIYAHSRLRV RCGTTYHLQNANICMATQTY c c g g c a g c c c g t g g t g t a a t g c a g c t g g t t a g c a t t t a t g c a c a t a g c c g t c t g c g t g t a g g c c g t c g g g c a c c a c a t t a c g t c g a c c a a t c g t a a a t a c g t g t a t c g g c a g a c g c a c a t

2100 LSRPKPIRRNRLTPRECEIL QATRFRNTTITQCRTFTFNQ c t g a g c c g t c c g a a a c c g a t t c g t c g t a a t c g t c t g a c a c c g c g t g a a t g t g a a a t t c t g g a c t c g g c a g g c t t t g g c t a a g c a g c a t t a g c a g a c t g t g g c g c a c t t a c a c t t t a a g a c

2160 QWAAQGKTAWEISVILCITE LPCCLTFGCPFNANNQTNGF c a g t g g g c a g c a c a g g g t a a a a c c g c a t g g g a a a t t a g c g t t a t t c t g t g t a t t a c c g a a g t c a c c c g t c g t g t c c c a t t t t g g c g t a c c c t t t a a t c g c a a t a a g a c a c a t a a t g g c t t

2220 RTVKFHLIEAARKLDAANRT AGNFKM c g c a c c g t t a a a t t t c a t c t g a t t g a a g c a g c a c g t a a a c t g g a t g c a g c a a a t c g t a c c g c g t g g c a a t t t a a a g t a g a c t a a c t t c g t c g t g c a t t t g a c c t a c g t c g t t t a g c a t g g

BstAPI 2280 130 PKKKRK SV40 NLS AAVAKALTLGLIRLPKKKRK g c a g c a g t t g c a a a a g c a c t g a c a c t g g g t c t g a t t c g t c t g c c g a a g a a g a a g a g g a a g c g t c g t c a a c g t t t t c g t g a c t g t g a c c c a g a c t a a g c a g a c g g c t t c t t c t t c t c c t t c

2340 V * SV40 NLS CaMV poly(A) signal V* g t t t a g t g t c g g t a c g c t g a a a t c a c c a g t c t c t c t c t a c a a a t c t a t c t c t c t c t a t t t c a a a t c a c a g c c a t g c g a c t t t a g t g g t c a g a g a g a g a t g t t t a g a t a g a g a g a g a t a a a

2400 CaMV poly(A) signal t c t c c a t a a a t a a t g t g t g a g t a g t t t c c c g a t a a g g g a a a t t a g g g t t c t t a t a g g g t t a g a g g t a t t t a t t a c a c a c t c a t c a a a g g g c t a t t c c c t t t a a t c c c a a g a a t a t c c c a a

2460 CaMV poly(A) signal t c g c t c a t g t g t t g a g c a t a t a a g a a a c c c t t a g t a t g t a t t t g t a t t t g t a a a a t a c t t a g c g a g t a c a c a a c t c g t a t a t t c t t t g g g a a t c a t a c a t a a a c a t a a a c a t t t t a t g a a

ScaI 2520 CaMV poly(A) signal c t a t c a a t a a a a t t t c t a a t t c c t a a a a c c a a a a t c c a g t a c t a a a a t c c a g a t c g a t g c g a t a g t t a t t t t a a a g a t t a a g g a t t t t g g t t t t a g g t c a t g a t t t t a g g t c t a g c t a c g

AleINotISpeI 2580 t t g a t a c t a c g t c t t c g g g c t a g a g c g g a c t a g t t c t a g a g c g g c c g c c a c c g c g g t g g a a a c t a t g a t g c a g a a g c c c g a t c t c g c c t g a t c a a g a t c t c g c c g g c g g t g g c g c c a c c t

2640 T3 promoter M13 rev G G A A A T C A C T C C C A A T T A A C G T3 C A G T A M13 Reverse g c t c c a g c t t t t g t t c c c t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t c g a g g t c g a a a a c a a g g g a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a

2700 -10 M13 rev lac operator lac promoter T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t

2760 -35 lac promoter CAP binding site g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g

2820 g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g

TaqIIBspQISapI 2880 G A G C G A G T G A C T G A L4440 a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a

2940 G C G A L4440 c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g

StuI 3000 RB T-DNA repeat g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g a a g g c c t t g a c a g g a t a t a c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c t t c c g g a a c t g t c c t a t a t

BglII 3060 RB T-DNA repeat t t g g c g g g t a a a c t a a g t c g c t g t a t g t g t t t g t t t g a g a t c t c a t g t g a g c a a a a g g c c a a c c g c c c a t t t g a t t c a g c g a c a t a c a c a a a c a a a c t c t a g a g t a c a c t c g t t t t c c g g

3120 ori a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g

3180 ori c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g

3240 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g

3300 ori t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t

3360 ori g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g

3420 ori a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t

AlwNI 3480 ori a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c

3540 ori c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t

3600 ori g a a g a a c a g t a t t t g g t a t c t g t g c t c t g c t g a a g c c a g t t a c c t t c g g a a g a a g a g t t g c t t c t t g t c a t a a a c c a t a g a c a c g a g a c g a c t t c g g t c a a t g g a a g c c t t c t t c t c a a c

3660 ori g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g

3720 ori a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a

EcoRI 3780 c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a a t t c t t g t t a g a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t t a a g a a c a a t c t

XmnI 3840 260 255 250 245 *KGVVKTIEGKVYHVFEEL SmR *KGVVKTIEGKVYHVFEEL c a t t a t t t g c c g a c t a c c t t g g t g a t c t c g c c t t t c a c g t a g t g a a c a a a t t c t t c c a a c g t a a t a a a c g g c t g a t g g a a c c a c t a g a g c g g a a a g t g c a t c a c t t g t t t a a g a a g g t t g

3900 240 235 230 225 QDARSALRDEQGLYAQRAEL SmR QDARSALRDEQGLYAQRAEL t g a t c t g c g c g c g a g g c c a a g c g a t c t t c t t g t c c a a g a t a a g c c t g c c t a g c t t c a a g t a c t a g a c g c g c g c t c c g g t t c g c t a g a a g a a c a g g t t c t a t t c g g a c g g a t c g a a g t t c a

3960 220 215 210 205 IVPQYQAPLREMAWDAAVDK SmR IVPQYQAPLREMAWDAAVDK a t g a c g g g c t g a t a c t g g g c c g g c a g g c g c t c c a t t g c c c a g t c g g c a g c g a c a t c c t t c t a c t g c c c g a c t a t g a c c c g g c c g t c c g c g a g g t a a c g g g t c a g c c g t c g c t g t a g g a a g

4020 200 195 190 185 PAIKGTVASYWIRSLTLVVN SmR PAIKGTVASYWIRSLTLVVN g g c g c g a t t t t g c c g g t t a c t g c g c t g t a c c a a a t g c g g g a c a a c g t a a g c a c t a c a t t t c c g c g c t a a a a c g g c c a a t g a c g c g a c a t g g t t t a c g c c c t g t t g c a t t c g t g a t g t a a a

4080 180 175 170 165 REDGAWDPPSNWLTLTENLA SmR REDGAWDPPSNWLTLTENLA c g c t c a t c g c c a g c c c a g t c g g g c g g c g a g t t c c a t a g c g t t a a g g t t t c a t t t a g c g c c g c g a g t a g c g g t c g g g t c a g c c c g c c g c t c a a g g t a t c g c a a t t c c a a a g t a a a t c g c g g

4140 160 155 150 145 EFLDQEPVPDFLEEAAPGVL SmR EFLDQEPVPDFLEEAAPGVL t c a a a t a g a t c c t g t t c a g g a a c c g g a t c a a a g a g t t c c t c c g c c g c t g g a c c t a c c a a g a g t t t a t c t a g g a c a a g t c c t t g g c c t a g t t t c t c a a g g a g g c g g c g a c c t g g a t g g t t c

4200 140 135 130 125 AVSHERAKTLLIALDIDITA SmR AVSHERAKTLLIALDIDITA g c a a c g c t a t g t t c t c t t g c t t t t g t c a g c a a g a t a g c c a g a t c a a t g t c g a t c g t g g c t c g t t g c g a t a c a a g a g a a c g a a a a c a g t c g t t c t a t c g g t c t a g t t a c a g c t a g c a c c g a

4260 120 115 110 105 PEFIGALIDNRQWEGFQLER SmR PEFIGALIDNRQWEGFQLER g g c t c g a a g a t a c c t g c a a g a a t g t c a t t g c g c t g c c a t t c t c c a a a t t g c a g t t c g c g c c c g a g c t t c t a t g g a c g t t c t t a c a g t a a c g c g a c g g t a a g a g g t t t a a c g t c a a g c g c g

4320 100 95 90 85 KAPYRWPIIDDHVVITVEVA SmR KAPYRWPIIDDHVVITVEVA t t a g c t g g a t a a c g c c a c g g a a t g a t g t c g t c g t g c a c a a c a a t g g t g a c t t c t a c a g c g a a t c g a c c t a t t g c g g t g c c t t a c t a c a g c a g c a c g t g t t g t t a c c a c t g a a g a t g t c g c

4380 80 75 70 65 RLIESEGPSASTELLDNILA SmR RLIESEGPSASTELLDNILA c g g a g a a t c t c g c t c t c t c c a g g g g a a g c c g a a g t t t c c a a a a g g t c g t t g a t c a a a g c t g c c t c t t a g a g c g a g a g a g g t c c c c t t c g g c t t c a a a g g t t t t c c a g c a a c t a g t t t c g a

BstEII 4440 60 55 50 45 RRTTEDLRVTVTVLLDIDSH SmR RRTTEDLRVTVTVLLDIDSH c g c c g c g t t g t t t c a t c a a g c c t t a c g g t c a c c g t a a c c a g c a a a t c a a t a t c a c t g t g t g c g g c g c a a c a a a g t a g t t c g g a a t g c c a g t g g c a t t g g t c g t t t a g t t a t a g t g a c a c a

4500 40 35 30 25 PKLGGDVASGYLHVALLTPE SmR PKLGGDVASGYLHVALLTPE g g c t t c a g g c c g c c a t c c a c t g c g g a g c c g t a c a a a t g t a c g g c c a g c a a c g t c g g t t c g c c g a a g t c c g g c g g t a g g t g a c g c c t c g g c a t g t t t a c a t g c c g g t c g t t g c a g c c a a g c

4560 20 15 10 5 LHREIVGVVESLQTSVEAIV SmR LHREIVGVVESLQTSVEAIV a g a t g g c g c t c g a t g a c g c c a a c t a c c t c t g a t a g t t g a g t c g a t a c t t c g g c g a t c a c c t c t a c c g c g a g c t a c t g c g g t t g a t g g a g a c t a t c a a c t c a g c t a t g a a g c c g c t a g t g g

4620 1 AERM SmR AERM g c t t c c c t c a t g a t g t t t a a c t c c t g a a t t a a g c c g c g c c g c g a a g c g g t g t c g g c t t g a c g a a g g g a g t a c t a c a a a t t g a g g a c t t a a t t c g g c g c g g c g c t t c g c c a c a g c c g a a c t

4680 a t g a a t t g t t a g g c g t c a t c c t g t g c t c c c g a g a a c c a g t a c c a g t a c a t c g c t g t t t c g t a c t t a a c a a t c c g c a g t a g g a c a c g a g g g c t c t t g g t c a t g g t c a t g t a g c g a c a a a g c

4740 t t c g a g a c t t g a g g t c t a g t t t t a t a c g t g a a c a g g t c a a t g c c g c c g a g a g t a a a g c c a a a g c t c t g a a c t c c a g a t c a a a a t a t g c a c t t g t c c a g t t a c g g c g g c t c t c a t t t c g g t

4800 c a t t t t g c g t a c a a a t t g c a g g c a g g t a c a t t g t t c g t t t g t g c c a t g a t g a t a t a t t t t g t a a a a c g c a t g t t t a a c g t c c g t c c a t g t a a c a a g c a a a c a c g g t a c t a c t a t a t a a a a

4860 t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a c a a c g t g g c t t t g t t g a a t a a a t c a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t g t g t t g c a c c g a a a c a a c t t a t t t a g

4920 g a a c t t t t g c t g a g t t g a a g g a t c a g a t c a c g c a t c t t c c c g a c a a c g c a g a c c g t t c c g c t t g a a a a c g a c t c a a c t t c c t a g t c t a g t g c g t a g a a g g g c t g t t g c g t c t g g c a a g g c

4980 t g g c a a a g c a a a a g t t c a a a a t c a c c a a c t g g t c c a c c t a c a a c a a a g c t c t c a t c a a c c a c c g t t t c g t t t t c a a g t t t t a g t g g t t g a c c a g g t g g a t g t t g t t t c g a g a g t a g t t g g

5040 pSa ori g t g g c t c c c t c a c t t t c t g g c t g g a t g a t g g g g c g a t t c a g g a g a t c c c c a t c c a a c a g c c a c c g a g g g a g t g a a a g a c c g a c c t a c t a c c c c g c t a a g t c c t c t a g g g g t a g g t t g t c g

5100 pSa ori c c g c c g t c g a g c g g g c t t t t t t a t c c c c g g a a g c c t g t g g a t a g a g g g t a g t t a t c c a c g g g c g g c a g c t c g c c c g a a a a a a t a g g g g c c t t c g g a c a c c t a t c t c c c a t c a a t a g g t g c

5160 pSa ori t g a a a c c g c t a a t g c c c c g c a a a g c c t t g a t t c a c g g g g c t t t c c g g c c c g c t c c a a a a a a c t t t g g c g a t t a c g g g g c g t t t c g g a a c t a a g t g c c c c g a a a g g c c g g g c g a g g t t t t t

5220 pSa ori c t a t c c a c g t g a a a t c g c t a a t c a g g g t a c g t g a a a t c g c t a a t c g g a g t a c g t g a a a t c g a t a g g t g c a c t t t a g c g a t t a g t c c c a t g c a c t t t a g c g a t t a g c c t c a t g c a c t t t a g

5280 pSa ori g c t a a t a a g g t c a c g t g a a a t c g c t a a t c a a a a a g g c a c g t g a g a a c g c t a a t a g c c c t t c g a t t a t t c c a g t g c a c t t t a g c g a t t a g t t t t t c c g t g c a c t c t t g c g a t t a t c g g g a a

5340 pSa ori t c a g a t c a a c a g c t t g c a a a c a c c c c t c g c t c c g g c a a g t a g t t a c a g c a a g t a g t a t g t a g t c t a g t t g t c g a a c g t t t g t g g g g a g c g a g g c c g t t c a t c a a t g t c g t t c a t c a t a c a

5400 pSa ori t c a a t t a g c t t t t c a a t t a t g a a t a t a t a t a t c a a t t a t t g g t c g c c c t t g g c t t g t g g a a g t t a a t c g a a a a g t t a a t a c t t a t a t a t a t a g t t a a t a a c c a g c g g g a a c c g a a c a c c t

5460 pSa ori c a a t g c g c t a c g c g c a c c g g c t c c g c c c g t g g a c a a c c g c a a g c g g t t g c c c a c c g t c g a g t t a c g c g a t g c g c g t g g c c g a g g c g g g c a c c t g t t g g c g t t c g c c a a c g g g t g g c a g c t

PsiIFseI 5520 pSa ori g c g c c t t t g c c c a c a a c c c g g c g g c c g g c c g c a a c a g a t c g t t t t a t a a a t t t t t t t t t t c g c g g a a a c g g g t g t t g g g c c g c c g g c c g g c g t t g t c t a g c a a a a t a t t t a a a a a a a a a a

5580 t g a a a a a g a a a a a g c c c g a a a g g c g g c a a c c t c t c g g g c t t c t g g a t t t c c g a t c c c c g g a c t t t t t c t t t t t c g g g c t t t c c g c c g t t g g a g a g c c c g a a g a c c t a a a g g c t a g g g g c c

5640 a a t t a g a c t c t g g c t g c c t g t a t c g a g t g g t g a t t t t g t g c c g a g c t g c c g g t c g g g g a g t t a a t c t g a g a c c g a c g g a c a t a g c t c a c c a c t a a a a c a c g g c t c g a c g g c c a g c c c c t c

5651 c t g t t g g c t g g g a c a a c c g a c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.