Addgene TALEN1260 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: TALEN1260 Sequencing Result

Map View

800 600 400 200 End (914) BsrDI (892) StyI (856) HpaI - HincII (806) PflMI (793) NspI (780) BclI * (712) AvaI - BsoBI (686) EciI (684) BamHI (659) SpeI (651) BpmI Eco57MI (620) BspHI (588) AseI (574) EcoRI (538) MslI (479) MmeI (462) BciVI (416) HindIII (397) PsiI (383) MscI (368) DraI (235) BlpI (215) EarI - SapI - BspQI (164) NaeI (127) NgoMIV (125) ApaI - BanII - BaeGI - Bsp1286I - Bme1580I (66) PspOMI (62) BstBI (57) TaqII (41) BsaI (17) MluI - AflIII (11) AgeI (5) Start (0) V5 tag FokI cleavage domain TALEN1260 914 bp

Sequence View

BstBITaqIIBsaIAflIIIMluIStart (0) AgeI 5ʹ 3ʹ 60 HGTR 10 5 1 TSDLGLLPNPIPKG V5 tag g a t g a c c g g t a c g c g t a g a a t c g a g a c c g a g g a g a g g g t t a g g g a t a g g c t t a c c t t c g a c t a c t g g c c a t g c g c a t c t t a g c t c t g g c t c c t c t c c c a a t c c c t a t c c g a a t g g a a g c t

PspOMIBme1580IBsp1286IBaeGIBanIIApaI 120 * 580 575 570 FNIEGNNFKRRVEELTL FokI cleavage domain *FNIEGNNFKRRVEELTL a g g g c c c t t a a a a g t t t a t c t c g c c g t t a t t a a a t t t c c g t c t g a c t t c c t c t a a g g t t a t c c c g g g a a t t t t c a a a t a g a g c g g c a a t a a t t t a a a g g c a g a c t g a a g g a g a t t c c a a t

BspQISapIEarINgoMIVNaeI 180 565 560 555 550 TGAKIMEGGILLEEVSLVAG FokI cleavage domain TGAKIMEGGILLEEVSLVAG a t g t g c c g g c t t t a a t c a t t t c t c c a c c a a t t a a a a g c t c t t c t a c a c t a a g a a c a g c t c t a c a c g g c c g a a a t t a g t a a a g a g g t g g t t a a t t t t c g a g a a g a t g t g a t t c t t g t c g a g

DraIBlpI 240 545 540 535 530 NCNTIHNLRTLQAKYNGKFH FokI cleavage domain NCNTIHNLRTLQAKYNGKFH c a t t a c a a t t a g t g a t a t g a t t t a a t c g t g t a a g c t g a g c t t t g t a g t t t c c t t t a a a g t g t a a t g t t a a t c a c t a t a c t a a a t t a g c a c a t t c g a c t c g a a a c a t c a a a g g a a a t t t c a

300 525 520 515 510 GSVFLFKFETVSSPYVKWWE FokI cleavage domain GSVFLFKFETVSSPYVKWWE g a c c a c t c a c a a a t a a a a a c t t a a a t t c c g t t a c a g a a g a t g g a t a g a c t t t c c a c c a t t c t g g t g a g t g t t t a t t t t t g a a t t t a a g g c a a t g t c t t c t a c c t a t c t g a a a g g t g g t a a

360 505 500 495 490 NPNIHKNRTQNEEVYRQMED FokI cleavage domain NPNIHKNRTQNEEVYRQMED c a t t a g g g t t g a t a t g t t t g t t t c g t g t t t g a t t t t c t t c g a c a t a t c g t t g c a t t t c a t g t a a t c c c a a c t a t a c a a a c a a a g c a c a a a c t a a a a g a a g c t g t a t a g c a a c g t a a a g t a

BciVIHindIIIPsiIMscI 420 485 480 475 470 AQGIPLNYGGSYAKTDVIVG FokI cleavage domain AQGIPLNYGGSYAKTDVIVG c t g c t t g g c c a a t t g g c a g a t t a t a a c c t c c g c t a t a a g c t t t a g t a t c c a c g a t c a c a c g a c g a a c c g g t t a a c c g t c t a a t a t t g g a g g c g a t a t t c g a a a t c a t a g g t g c t a g t g t g

MslIMmeI 480 465 460 455 450 YDIPSGVTYIAGDPKRSGGL FokI cleavage domain YDIPSGVTYIAGDPKRSGGL c g t a a t c a a t a g g a g a t c c g a c a g t a t a a a t t g c t c c g t c c g g t t t c c t t g a t c c a c c c a g c a t t a g t t a t c c t c t a g g c t g t c a t a t t t a a c g a g g c a g g c c a a a g g a a c t a g g t g g g t

EcoRI 540 445 440 435 430 HKGRYGYVKMFFEMVKMELI FokI cleavage domain HKGRYGYVKMFFEMVKMELI a a t g t t t a c c t c t a t a t c c a t a a a c t t t c a t a a a a a a t t c c a t t a c c t t c a t t t c a a g a a t t a c a a a t g g a g a t a t a g g t a t t t g a a a g t a t t t t t t a a g g t a a t g g a a g t a a a g t t c t t

BspHIAseI 600 425 420 415 410 RDQTSNRAIEILEIYEHPVY FokI cleavage domain RDQTSNRAIEILEIYEHPVY t t c t a t c c t g a g t g g a a t t t c t g g c a a t t t c a a t t a a t t c a a t a t a t t c a t g a g g c a c a t a a g a t a g g a c t c a c c t t a a a g a c c g t t a a a g t t a a t t a a g t t a t a t a a g t a c t c c g t g t a

BamHISpeIEco57MIBpmI 660 405 400 395 390 KLKHRLESKKEELESKVLQ FokI cleavage domain KLKHRLESKKEELESKVLQS a t t t c a a t t t a t g a c g a a g t t c a g a t t t c t t c t c c t c c a g t t c a c t t t t g a c t a g t t g g g t a a a g t t a a a t a c t g c t t c a a g t c t a a a g a a g a g g a g g t c a a g t g a a a a c t g a t c a a c c c

BclI*EciIBsoBIAvaI 720 GAVRHSTREPIRRNTRKILA a t c c c g c g a c t c g a t g g g a a g t t c t c t c g g g a a t c c g c c g g t t g g t t c t t t t g a t c a a t g t a g g g c g c t g a g c t a c c c t t c a a g a g a g c c c t t a g g c g g c c a a c c a a g a a a a c t a g t t a c

NspI 780 PAHPLGKKVADLAPRGGLCA c g g g a g c a t g a g g c a g a c c c t t t t t g a c t g c a t c g a g c g c g g g t c g t c c a c c a a g a c a t g g c c c t c g t a c t c c g t c t g g g a a a a a c t g a c g t a g c t c g c g c c c a g c a g g t g g t t c t g t a c

HincIIHpaIPflMI 840 LAVLHDNTLAALAPDPRSLQ c c a a c g c c a c c a g a t g g t c a t t c g t t a a c g c a g c c a a c g c g g g a t c g g g c c t g g a c a a t t g g t t g c g g t g g t c t a c c a g t a a g c a a t t g c g t c g g t t g c g c c c t a g c c c g g a c c t g t t a a

BsrDIStyI 900 AVISELAPRGGDHSAIAVVQ g g g c t a c g a t g g a c t c c a a g g c t g g c c g t c c t c c g t c g t g g g a a g c a a t g g c g a c c a c c t c c c g a t g c t a c c t g a g g t t c c g a c c g g c a g g a g g c a g c a c c c t t c g t t a c c g c t g g t g g a

End (914) 3ʹ 5ʹ 914 EPTL g t t c g g g t g t c a g c c a a g c c c a c a g t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.