Addgene pMET7-GAG-FRB Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pMET7-GAG-FRB Sequencing Result

Map View

NheI (21) NaeI (4647) NgoMIV (4645) Bpu10I (4590) Bsu36I (4496) SfiI (4380) CsiI - SexAI * (4194) pBABE 3' (4108 .. 4128) BfuAI - BspMI - PaqCI (4099) SspI (3980) Amp-R (3726 .. 3745) BsaHI (3713) ScaI (3656) FspI (3398) pBR322ori-F (2424 .. 2443) DrdI (2391) BmtI (25) BspDI * - ClaI * (74) BspQI - SapI (343) PvuII (390) PstI (662) BstXI (878) KflI (1095) BglII (1339) SalI (1538) EcoRI (1614) EagI - NotI (1921) PsiI (2019) SV40pA-R (2037 .. 2056) EBV-rev (2091 .. 2110) AvaI - BsoBI - TspMI - XmaI (2135) SmaI (2137) Acc65I (2139) KpnI (2143) Eco53kI (2147) SacI (2149) L4440 (2173 .. 2190) AflIII - PciI (2283) pMET7-GAG-FRB 4876 bp

Sequence View

BmtINheI 5Ź¹ 3Ź¹ 60 1 5 MGARASVLS HIV-1 gag Kozak sequence LKAAGPASATMGARASVLS c t c t a a a a g c t g c g g g g c c c g c t a g c g c c a c c a t g g g t g c g a g a g c g t c a g t a t t a a g c g g a g a t t t t c g a c g c c c c g g g c g a t c g c g g t g g t a c c c a c g c t c t c g c a g t c a t a a t t c g c

BspDI*ClaI* 120 10 15 20 25 GGELDRWEKIRLRPGGKKKY HIV-1 gag GGELDRWEKIRLRPGGKKKY g g g g a g a a t t a g a t c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a c c c c t c t t a a t c t a g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t

180 30 35 40 45 KLKHIVWASRELERFAVNPG HIV-1 gag KLKHIVWASRELERFAVNPG a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g

240 50 55 60 65 LLETSEGCRQILGQLQPSLQ HIV-1 gag LLETSEGCRQILGQLQPSLQ t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t

300 70 75 80 85 TGSEELRSLYNTVATLYCVH HIV-1 gag TGSEELRSLYNTVATLYCVH c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g

BspQISapI 360 90 95 100 105 QRIEIKDTKEALDKIEEEQN HIV-1 gag QRIEIKDTKEALDKIEEEQN a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t

PvuII 420 110 115 120 125 KSKKKAQQAAADTGHSNQVS HIV-1 gag KSKKKAQQAAADTGHSNQVS a a a g t a a g a a a a a a g c a c a g c a a g c a g c a g c t g a c a c a g g a c a c a g c a a t c a g g t c a g c c t t t c a t t c t t t t t t c g t g t c g t t c g t c g t c g a c t g t g t c c t g t g t c g t t a g t c c a g t c g g

480 130 135 140 145 QNYPIVQNIQGQMVHQAISP HIV-1 gag QNYPIVQNIQGQMVHQAISP a a a a t t a c c c t a t a g t g c a g a a c a t c c a g g g g c a a a t g g t a c a t c a g g c c a t a t c a c c t a t t t t a a t g g g a t a t c a c g t c t t g t a g g t c c c c g t t t a c c a t g t a g t c c g g t a t a g t g g a t

540 150 155 160 165 RTLNAWVKVVEEKAFSPEVI HIV-1 gag RTLNAWVKVVEEKAFSPEVI g a a c t t t a a a t g c a t g g g t a a a a g t a g t a g a a g a g a a g g c t t t c a g c c c a g a a g t g a t a c c t t g a a a t t t a c g t a c c c a t t t t c a t c a t c t t c t c t t c c g a a a g t c g g g t c t t c a c t a t g

600 170 175 180 185 PMFSALSEGATPQDLNTMLN HIV-1 gag PMFSALSEGATPQDLNTMLN c c a t g t t t t c a g c a t t a t c a g a a g g a g c c a c c c c a c a a g a t t t a a a c a c c a t g c t a a a c a g g t a c a a a a g t c g t a a t a g t c t t c c t c g g t g g g g t g t t c t a a a t t t g t g g t a c g a t t t g t

660 190 195 200 205 TVGGHQAAMQMLKETINEEA HIV-1 gag TVGGHQAAMQMLKETINEEA c a g t g g g g g g a c a t c a a g c a g c c a t g c a a a t g t t a a a a g a g a c c a t c a a t g a g g a a g c t g g t c a c c c c c c t g t a g t t c g t c g g t a c g t t t a c a a t t t t c t c t g g t a g t t a c t c c t t c g a c

PstI 720 210 215 220 225 AEWDRVHPVHAGPIAPGQMR HIV-1 gag AEWDRVHPVHAGPIAPGQMR c a g a a t g g g a t a g a g t g c a t c c a g t g c a t g c a g g g c c t a t t g c a c c a g g c c a g a t g a g a g g t c t t a c c c t a t c t c a c g t a g g t c a c g t a c g t c c c g g a t a a c g t g g t c c g g t c t a c t c t c

780 230 235 240 245 EPRGSDIAGTTSTLQEQIGW HIV-1 gag EPRGSDIAGTTSTLQEQIGW a a c c a a g g g g a a g t g a c a t a g c a g g a a c t a c t a g t a c c c t t c a g g a a c a a a t a g g a t g g a t t g g t t c c c c t t c a c t g t a t c g t c c t t g a t g a t c a t g g g a a g t c c t t g t t t a t c c t a c c t

840 250 255 260 265 MTHNPPIPVGEIYKRWIILG HIV-1 gag MTHNPPIPVGEIYKRWIILG t g a c a c a t a a t c c a c c t a t c c c a g t a g g a g a a a t c t a t a a a a g a t g g a t a a t c c t g g g a t a c t g t g t a t t a g g t g g a t a g g g t c a t c c t c t t t a g a t a t t t t c t a c c t a t t a g g a c c c t a

BstXI 900 270 275 280 285 LNKIVRMYSPTSILDIRQGP HIV-1 gag LNKIVRMYSPTSILDIRQGP t a a a t a a a a t a g t a a g a a t g t a t a g c c c t a c c a g c a t t c t g g a c a t a a g a c a a g g a c c a a a t t t a t t t t a t c a t t c t t a c a t a t c g g g a t g g t c g t a a g a c c t g t a t t c t g t t c c t g g t t

960 290 295 300 305 KEPFRDYVDRFYKTLRAEQA HIV-1 gag KEPFRDYVDRFYKTLRAEQA a g g a a c c c t t t a g a g a c t a t g t a g a c c g a t t c t a t a a a a c t c t a a g a g c c g a g c a a g c t t t c c t t g g g a a a t c t c t g a t a c a t c t g g c t a a g a t a t t t t g a g a t t c t c g g c t c g t t c g a a

1020 310 315 320 325 SQEVKNWMTETLLVQNANPD HIV-1 gag SQEVKNWMTETLLVQNANPD c a c a a g a g g t a a a a a a t t g g a t g a c a g a a a c c t t g t t g g t c c a a a a t g c g a a c c c a g a t t g t g t t c t c c a t t t t t t a a c c t a c t g t c t t t g g a a c a a c c a g g t t t t a c g c t t g g g t c t a a

1080 330 335 340 345 CKTILKALGPGATLEEMMTA HIV-1 gag CKTILKALGPGATLEEMMTA g t a a g a c t a t t t t a a a a g c a t t g g g a c c a g g a g c g a c a c t a g a a g a a a t g a t g a c a g c a t c a t t c t g a t a a a a t t t t c g t a a c c c t g g t c c t c g c t g t g a t c t t c t t t a c t a c t g t c g t a

KflI 1140 350 355 360 365 CQGVGGPGHKARVLAEAMSQ HIV-1 gag CQGVGGPGHKARVLAEAMSQ g t c a g g g a g t g g g g g g a c c c g g c c a t a a a g c a a g a g t t t t g g c t g a a g c a a t g a g c c a a g c a g t c c c t c a c c c c c c t g g g c c g g t a t t t c g t t c t c a a a a c c g a c t t c g t t a c t c g g t t c

1200 370 375 380 385 VTNPATIMIQKGNFRNQRKT HIV-1 gag VTNPATIMIQKGNFRNQRKT t a a c a a a t c c a g c t a c c a t a a t g a t a c a g a a a g g c a a t t t t a g g a a c c a a a g a a a g a c t g a t t g t t t a g g t c g a t g g t a t t a c t a t g t c t t t c c g t t a a a a t c c t t g g t t t c t t t c t g a c

1260 390 395 400 405 VKCFNCGKEGHIAKNCRAPR HIV-1 gag VKCFNCGKEGHIAKNCRAPR t t a a g t g t t t c a a t t g t g g c a a a g a a g g g c a c a t a g c c a a a a a t t g c a g g g c c c c t a g g a a a t t c a c a a a g t t a a c a c c g t t t c t t c c c g t g t a t c g g t t t t t a a c g t c c c g g g g a t c c t

1320 410 415 420 425 KKGCWKCGKEGHQMKDCTER HIV-1 gag KKGCWKCGKEGHQMKDCTER a a a a g g g c t g t t g g a a a t g t g g a a a g g a a g g a c a c c a a a t g a a a g a t t g t a c t g a g a g a c t t t t c c c g a c a a c c t t t a c a c c t t t c c t t c c t g t g g t t t a c t t t c t a a c a t g a c t c t c t g

BglII 1380 430 435 440 445 QANFLGKIWPSHKGRPGNFL HIV-1 gag QANFLGKIWPSHKGRPGNFL a g g c t a a t t t t t t a g g g a a g a t c t g g c c t t c c c a c a a g g g a a g g c c a g g g a a t t t t c t t c t c c g a t t a a a a a a t c c c t t c t a g a c c g g a a g g g t g t t c c c t t c c g g t c c c t t a a a a g a a g

1440 450 455 460 465 QSRPEPTAPPEESFRFGEET HIV-1 gag QSRPEPTAPPEESFRFGEET a g a g c a g a c c a g a g c c a a c a g c c c c a c c a g a a g a g a g c t t c a g g t t t g g g g a a g a g a c a a t c t c g t c t g g t c t c g g t t g t c g g g g t g g t c t t c t c t c g a a g t c c a a a c c c c t t c t c t g t t

1500 470 475 480 485 TTPSQKQEPIDKELYPLASL HIV-1 gag TTPSQKQEPIDKELYPLASL c a a c t c c c t c t c a g a a g c a g g a g c c g a t a g a c a a g g a a c t g t a t c c t t t a g c t t c c c t c a g t t g a g g g a g a g t c t t c g t c c t c g g c t a t c t g t t c c t t g a c a t a g g a a a t c g a a g g g a g t

SalI 1560 490 495 500 RSLFGSDPSSQ (in frame with HIV-1 gag) GGSTLTLIF HIV-1 gag RSLFGSDPSSQGGSTLTLIF g a t c a c t c t t t g g c a g c g a c c c c t c g t c a c a a g g c g g g t c g a c g c t g a c c c t g a t c t t c a c t a g t g a g a a a c c g t c g c t g g g g a g c a g t g t t c c g c c c a g c t g c g a c t g g g a c t a g a a g t

EcoRI 1620 (in frame with HIV-1 gag) REAVSEILETSRFAYLYDEF REAVSEILETSRFAYLYDEF g a g a g g c c g t g a g c g a a a t c c t g g a g a c c t c c a g a t t c g c c t a c c t g t a c g a c g a a t t c a c t c t c c g g c a c t c g c t t t a g g a c c t c t g g a g g t c t a a g c g g a t g g a c a t g c t g c t t a a g t

1680 MASR 2025 2030 2035 ILWHEMWHEGLEEASR FRB* MASRILWHEMWHEGLEEASR t g g c t t c t a g a a t c c t c t g g c a t g a g a t g t g g c a t g a a g g c c t g g a a g a g g c a t c t c g t t a c c g a a g a t c t t a g g a g a c c g t a c t c t a c a c c g t a c t t c c g g a c c t t c t c c g t a g a g c a a

1740 2040 2045 2050 2055 LYFGERNVKGMFEVLEPLHA FRB* LYFGERNVKGMFEVLEPLHA t g t a c t t t g g g g a a a g g a a c g t g a a a g g c a t g t t t g a g g t g c t g g a g c c c t t g c a t g c t a a c a t g a a a c c c c t t t c c t t g c a c t t t c c g t a c a a a c t c c a c g a c c t c g g g a a c g t a c g a t

1800 2060 2065 2070 2075 MMERGPQTLKETSFNQAYGR FRB* MMERGPQTLKETSFNQAYGR t g a t g g a a c g g g g c c c c c a g a c t c t g a a g g a a a c a t c c t t t a a t c a g g c c t a t g g t c g a g a c t a c c t t g c c c c g g g g g t c t g a g a c t t c c t t t g t a g g a a a t t a g t c c g g a t a c c a g c t c

1860 2080 2085 2090 2095 DLMEAQEWCRKYMKSGNVKD FRB* DLMEAQEWCRKYMKSGNVKD a t t t a a t g g a g g c c c a a g a g t g g t g c a g g a a g t a c a t g a a a t c a g g g a a t g t c a a g g a c c t a a a t t a c c t c c g g g t t c t c a c c a c g t c c t t c a t g t a c t t t a g t c c c t t a c a g t t c c t g g

1920 2100 2105 2110 LLQAWDLYYHVFRRISK TS* FRB* LLQAWDLYYHVFRRISKTS* t c c t c c a a g c c t g g g a c c t c t a t t a t c a t g t g t t c c g a c g a a t c t c a a a g a c t a g t t a a g a g g a g g t t c g g a c c c t g g a g a t a a t a g t a c a c a a g g c t g c t t a g a g t t t c t g a t c a a t t c

EagINotI 1980 c g g c c g c a t a a t c t a g a g a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g c c g g c g t a t t a g a t c t c t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a

PsiI 2040 SV40 poly(A) signal C G T T SV40pA-R g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t

2100 SV40 poly(A) signal G T G G T T T G T C EBV-rev A T C G T A G T G T T T A A A G SV40pA-R t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g

TspMIBsoBIXmaIAvaISmaIAcc65IKpnIEco53kISacI 2160 SV40 poly(A) signal C A A A C T C A T C EBV-rev c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c c c c g g g t a c c g a g c t c g a a t t a a t t c g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g g g g c c c a t g g c t c g a g c t t a a t t a a g

2220 G A G C G A G T G A C T G A G C G A L4440 c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a

2280 a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t

AflIIIPciI 2340 g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g

DrdI 2400 ori g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c

2460 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a

2520 ori g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c

2580 ori a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c

2640 ori c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c

2700 ori t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g

2760 ori t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c

2820 ori g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a

2880 ori t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c

2940 ori t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g

3000 t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a

3060 g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a

3120 285 *WHKIL AmpR *WHKIL t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c

3180 280 275 270 265 SAGIEAIQRNREDMTAQSGT AmpR SAGIEAIQRNREDMTAQSGT t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a

3240 260 255 250 245 TYIVVIRSPKGDPGLAAIIG AmpR TYIVVIRSPKGDPGLAAIIG c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g

3300 240 235 230 225 RSGREGAGSKDAIFWGAPLA AmpR RSGREGAGSKDAIFWGAPLA g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g

3360 220 215 210 205 SRLLPGAVKDAEMWDILQQR AmpR SRLLPGAVKDAEMWDILQQR c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c

FspI 3420 200 195 190 185 SALTLLEGTLLKRLTTAMAV AmpR SALTLLEGTLLKRLTTAMAV g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g

3480 180 175 170 165 PMTTDREDNPIAENLEPEWR AmpR PMTTDREDNPIAENLEPEWR a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c

3540 160 155 150 145 DLRTVHDGMNHLFATLEKPG AmpR DLRTVHDGMNHLFATLEKPG a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g

3600 140 135 130 125 GITTLLLNAATNDSMTIAAS AmpR GITTLLLNAATNDSMTIAAS t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a

ScaI 3660 120 115 110 105 CLERVTMGDTLHKETVPSYE AmpR CLERVTMGDTLHKETVPSYE g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g

BsaHI 3720 100 95 90 85 VLDNQSYHIRRGLQEQGADI AmpR VLDNQSYHIRRGLQEQGADI a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a

3780 80 75 70 65 RSLVAGCLLVKFTSMMPFRE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RSLVAGCLLVKFTSMMPFRE a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g

3840 60 55 50 45 EPRFSELIKGSNLDLEIYGV AmpR EPRFSELIKGSNLDLEIYGV t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g

3900 40 35 30 25 RAGLQDEADKVKVLTEPH signal se... AF AmpR RAGLQDEADKVKVLTEPHAF t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t

3960 20 15 10 5 signal sequence VPLCFAAFFPILAVRFHQIS AmpR VPLCFAAFFPILAVRFHQIS a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a

SspI 4020 1 sig... M AmpR AmpR promoter M c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c

4080 AmpR promoter a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c

BspMIPaqCIBfuAI 4140 SV40 promoter SR Ī± promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' a a a a g t g c c a c c t g c a a g c t t g g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c t t t t c a c g g t g g a c g t t c g a a c c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g

SexAI*CsiI 4200 SV40 promoter SR Ī± promoter c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a

4260 SV40 promoter SR Ī± promoter g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a

4320 SV40 promoter SR Ī± promoter SV40 ori c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c

SfiI 4380 SV40 promoter SR Ī± promoter SV40 ori c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a

4440 SV40 promoter SR Ī± promoter SV40 ori c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t

Bsu36I 4500 LTR sequences SR Ī± promoter 5' LTR (truncated) a a a a g c t c c t c g a t c g a g g g g c t c g c a t c t c t c c t t c a c g c g c c c g c t g c c c t a c c t g a g t t t t c g a g g a g c t a g c t c c c c g a g c g t a g a g a g g a a g t g c g c g g g c g a c g g g a t g g a c t c

4560 LTR sequences SR Ī± promoter 5' LTR (truncated) g c c g c c a t c c a c g c c g g t t g a g t c g c g t t c t g c c g c c t c c c g c c t g t g g t g c c t c c t g a a c g g c g g t a g g t g c g g c c a a c t c a g c g c a a g a c g g c g g a g g g c g g a c a c c a c g g a g g a c t t

Bpu10I 4620 LTR sequences SR Ī± promoter 5' LTR (truncated) c t g c g t c c g c c g t c t a g g t a a g t t t a a a g c t c a g g t c g a g a c c g g g c c t t t g t c c g g c g c g a c g c a g g c g g c a g a t c c a t t c a a a t t t c g a g t c c a g c t c t g g c c c g g a a a c a g g c c g c g

NgoMIVNaeI 4680 LTR sequences SR Ī± promoter 5' LTR (truncated) t c c c t t g g a g c c t a c c t a g a c t c a g c c g g c t c t c c a c g c t t t g c c t g a c c c t g c t t g c t c a g g g a a c c t c g g a t g g a t c t g a g t c g g c c g a g a g g t g c g a a a c g g a c t g g g a c g a a c g a g

4740 LTR sequences SR Ī± promoter 5' LTR (truncated) a a c t c t a c g t c t t t g t t t c g t t t t c t g t t c t g c g c c g t t a c a g a t c c a a g c t c t g a a a a a t t g a g a t g c a g a a a c a a a g c a a a a g a c a a g a c g c g g c a a t g t c t a g g t t c g a g a c t t t t t

4800 SV40 intron c c a g a a a g t t a a c t g g t a a g t t t a g t c t t t t t g t c t t t t a t t t c a g g t c c c g g a t c c g g t g g t c t t t c a a t t g a c c a t t c a a a t c a g a a a a a c a g a a a a t a a a g t c c a g g g c c t a g g c c a

4860 SV40 intron MLPLLLGLY g g t g g t g c a a a t c a a a g a a c t g c t c c t c a g t g g a t g t t g c c t t t a c t t c t a g g c c t g t a c c c a c c a c g t t t a g t t t c t t g a c g a g g a g t c a c c t a c a a c g g a a a t g a a g a t c c g g a c a t g

3Ź¹ 5Ź¹ 4876 GSVTS g g a a g t g t t a c t t c t g c c t t c a c a a t g a a g a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.