Addgene pTRANS_213 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pTRANS_213 Sequencing Result


Map View

pBR322ori-F (12,419 .. 12,438) L4440 (12,168 .. 12,185) pRS-marker (12,068 .. 12,087) pGEX 3' (11,905 .. 11,927) PluTI (11,856) SfoI (11,854) NarI (11,853) KasI (11,852) AgeI (11,561) StuI (11,465) FspI (11,284) BspDI * - ClaI * (11,147) BmtI (11,071) NheI (11,067) BsiWI (10,951) SphI (8063) PmeI (7938) SacI (7352) Eco53kI (7350) HindIII (7300) NruI (7060) NsiI (369) BspHI (903) SacII (1357) PspXI (1875) RsrII (2528) AsiSI (2577) AhdI (2878) 35S promoter (3050 .. 3070) CAP binding site M13/pUC Reverse (3953 .. 3975) lac operator M13 Reverse (3972 .. 3988) M13 rev lac UV5 promoter XbaI (5338) BssHII (5385) TspMI - XmaI (5760) SmaI - SrfI (5762) ccdB-fwd (5863 .. 5882) SalI (6019) BstEII (6147) pTRANS_213 12,635 bp

Sequence View

5ʹ 3ʹ 60 ori t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g

120 ori t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c

180 ori g c c t a a c t a c g g c t a c a c t a g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t c g g a t t g a t g c c g a t g t g a t c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a

240 ori t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c

300 ori t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g

360 t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a

NsiI 420 265 260 255 *FLEDLLIYYKIKE KanR *FLEDLLIYYKIKE g g t c a t g c a t t c t a g g t a c t a a a a c a a t t c a t c c a g t a a a a t a t a a t a t t t t a t t t t c t c c c a g t a c g t a a g a t c c a t g a t t t t g t t a a g t a g g t c a t t t t a t a t t a t a a a a t a a a a g a g

480 250 245 240 235 WDPKIGLLDFFLEVYQEEGI KanR WDPKIGLLDFFLEVYQEEGI c c a a t c a g g c t t g a t c c c c a g t a a g t c a a a a a a t a g c t c g a c a t a c t g t t c t t c c c c g a t g g t t a g t c c g a a c t a g g g g t c a t t c a g t t t t t t a t c g a g c t g t a t g a c a a g a a g g g g c t a

540 230 225 220 215 DERISRVCFAIDYWKDARGS KanR DERISRVCFAIDYWKDARGS a t c c t c c c t g a t c g a c c g g a c g c a g a a g g c a a t g t c a t a c c a c t t g t c c g c c c t g c c g c t t a g g a g g g a c t a g c t g g c c t g c g t c t t c c g t t a c a g t a t g g t g a a c a g g c g g g a c g g c g a

600 210 205 200 195 RGLDIFGSVKGDKVFINSDG KanR RGLDIFGSVKGDKVFINSDG t c t c c c a a g a t c a a t a a a g c c a c t t a c t t t g c c a t c t t t c a c a a a g a t g t t g c t g t c t c c a g a g g g t t c t a g t t a t t t c g g t g a a t g a a a c g g t a g a a a g t g t t t c t a c a a c g a c a g a g g

660 190 185 180 175 LDGHSFVLEEEPKETKLFDY KanR LDGHSFVLEEEPKETKLFDY c a g g t c g c c g t g g g a a a a g a c a a g t t c c t c t t c g g g c t t t t c c g t c t t t a a a a a a t c a t a g t c c a g c g g c a c c c t t t t c t g t t c a a g g a g a a g c c c g a a a a g g c a g a a a t t t t t t a g t a t

720 170 165 160 155 LERPDKFPTDEEWNECDVDA KanR LERPDKFPTDEEWNECDVDA c a g c t c g c g c g g a t c t t t a a a t g g a g t g t c t t c t t c c c a g t t t t c g c a a t c c a c a t c g g c g t c g a g c g c g c c t a g a a a t t t a c c t c a c a g a a g a a g g g t c a a a a g c g t t a g g t g t a g c c g

780 150 145 140 135 LDNNLLYDLEALRSDLSNTY KanR LDNNLLYDLEALRSDLSNTY c a g a t c g t t a t t c a g t a a g t a a t c c a a t t c g g c t a a g c g g c t g t c t a a g c t a t t c g t a t a g t c t a g c a a t a a g t c a t t c a t t a g g t t a a g c c g a t t c g c c g a c a g a t t c g a t a a g c a t a t

840 130 125 120 115 PCDSIDISHFLRICEAYLEI KanR PCDSIDISHFLRICEAYLEI g g g a c a a t c c g a t a t g t c g a t g g a g t g a a a g a g c c t g a t g c a c t c c g c a t a c a g c t c g a t c c c t g t t a g g c t a t a c a g c t a c c t c a c t t t c t c g g a c t a c g t g a g g c g t a t g t c g a g c t a

900 110 105 100 95 IKEPSQEDEYEESCLVGDAE KanR IKEPSQEDEYEESCLVGDAE a a t c t t t t c a g g g c t t t g t t c a t c t t c a t a c t c t t c c g a g c a a a g g a c g c c a t c g g c c t c t t a g a a a a g t c c c g a a a c a a g t a g a a g t a t g a g a a g g c t c g t t t c c t g c g g t a g c c g g a g

BspHI 960 90 85 80 75 SMLLNSWGDHREFHLVKPVP KanR SMLLNSWGDHREFHLVKPVP a c t c a t g a g c a g a t t g c t c c a g c c a t c a t g c c g t t c a a a g t g c a g g a c c t t t g g a a c a g g t g a g t a c t c g t c t a a c g a g g t c g g t a g t a c g g c a a g t t t c a c g t c c t g g a a a c c t t g t c c

1020 70 65 60 55 LKGELWLMMDKEREVDYTTG KanR LKGELWLMMDKEREVDYTTG c a g c t t t c c t t c c a g c c a t a g c a t c a t g t c c t t t t c c c g t t c c a c a t c a t a g g t g g t c c c g t c g a a a g g a a g g t c g g t a t c g t a g t a c a g g a a a a g g g c a a g g t g t a g t a t c c a c c a g g g

1080 50 45 40 35 KYRSDTMKLYLNENEGVLKY KanR KYRSDTMKLYLNENEGVLKY t t t a t a c c g g c t g t c c g t c a t t t t t a a a t a t a g g t t t t c a t t t t c t c c c a c c a g c t t a t a a a a t a t g g c c g a c a g g c a g t a a a a a t t t a t a t c c a a a a g t a a a a g a g g g t g g t c g a a t a t

1140 30 25 20 15 VKAPSMGETDKVCRYKEILK KanR VKAPSMGETDKVCRYKEILK t a c c t t a g c a g g a g a c a t t c c t t c c g t a t c t t t t a c g c a g c g g t a t t t t t c g a t c a g t t t a t g g a a t c g t c c t c t g t a a g g a a g g c a t a g a a a a t g c g t c g c c a t a a a a a g c t a g t c a a a

1200 10 5 1 KLEPSIRMKAM KanR KLEPSIRMKAM t t t c a a t t c c g g t g a t a t t c t c a t t t t a g c c a t t t a t t a t t t c c t t c c t c t t t t c t a c a g a a a g t t a a g g c c a c t a t a a g a g t a a a a t c g g t a a a t a a t a a a g g a a g g a g a a a a g a t g t c

1260 t a t t t a a a g a t a c c c c a a g a a g c t a a t t a t a a c a a g a c g a a c t c c a a t t c a c t g t t c c t t a t a a a t t t c t a t g g g g t t c t t c g a t t a a t a t t g t t c t g c t t g a g g t t a a g t g a c a a g g a a

1320 g c a t t c t a a a a c c t t a a a t a c c a g a a a a c a g c t t t t t c a a a g t t g t t t t c a a a g t t g g c g c g t a a g a t t t t g g a a t t t a t g g t c t t t t g t c g a a a a a g t t t c a a c a a a a g t t t c a a c c g c

SacII 1380 t a t a a c a t a g t a t c g a c g g a g c c g a t t t t g a a a c c g c g g t g a t c a c a g g c a g c a a c g c t c a t a t t g t a t c a t a g c t g c c t c g g c t a a a a c t t t g g c g c c a c t a g t g t c c g t c g t t g c g a g

1440 t g t c a t c g t t a c a a t c a a c a t g c t a c c c t c c g c g a g a t c a t c c g t g t t t c a a a c c c g g c a a c a g t a g c a a t g t t a g t t g t a c g a t g g g a g g c g c t c t a g t a g g c a c a a a g t t t g g g c c g t

1500 g c t t a g t t g c c g t t c t t c c g a a t a g c a t c g g t a a c a t g a g c a a a g t c t g c c g c c t t a c a a c g a a t c a a c g g c a a g a a g g c t t a t c g t a g c c a t t g t a c t c g t t t c a g a c g g c g g a a t g t t

1560 c g g c t c t c c c g c t g a c g c c g t c c c g g a c t g a t g g g c t g c c t g t a t c g a g t g g t g a t t t t g g c c g a g a g g g c g a c t g c g g c a g g g c c t g a c t a c c c g a c g g a c a t a g c t c a c c a c t a a a a c

1620 LB T-DNA repeat t g c c g a g c t g c c g g t c g g g g a g c t g t t g g c t g g c t g g t g g c a g g a t a t a t t g t g g t g t a a a c g g c t c g a c g g c c a g c c c c t c g a c a a c c g a c c g a c c a c c g t c c t a t a t a a c a c c a c a t t

1680 LB T-DNA repeat a c a a a t t g a c g c t t a g a c a a c t t a a t a a c a c a t t g c g g a c g t t t t t a a t g t a c t g a a t t a t g t t t a a c t g c g a a t c t g t t g a a t t a t t g t g t a a c g c c t g c a a a a a t t a c a t g a c t t a a t

1740 CaMV poly(A) signal a c g c c g a a t t a a t t c g g g g g a t c t g g a t t t t a g t a c t g g a t t t t g g t t t t a g g a a t t a g a t g c g g c t t a a t t a a g c c c c c t a g a c c t a a a a t c a t g a c c t a a a a c c a a a a t c c t t a a t c t

1800 CaMV poly(A) signal a a t t t t a t t g a t a g a a g t a t t t t a c a a a t a c a a a t a c a t a c t a a g g g t t t c t t a t a t g c t t t a a a a t a a c t a t c t t c a t a a a a t g t t t a t g t t t a t g t a t g a t t c c c a a a g a a t a t a c g a

1860 CaMV poly(A) signal c a a c a c a t g a g c g a a a c c c t a t a g g a a c c c t a a t t c c c t t a t c t g g g a a c t a c t c a c a c a g t t g t g t a c t c g c t t t g g g a t a t c c t t g g g a t t a a g g g a a t a g a c c c t t g a t g a g t g t g t

PspXI 1920 *K CaMV poly(A) signal HygR *K HygR *K t t a t t a t g g a g a a a c t c g a g c t t g t c g a t c g a c a g a t c c c g g t c g g c a t c t a c t c t a t t t a a t a a t a c c t c t t t g a g c t c g a a c a g c t a g c t g t c t a g g g c c a g c c g t a g a t g a g a t a a a

1980 340 335 330 325 KARPRTSPRRNGSDALVEVC HygR 340 335 330 325 KARPRTSPRRNGSDALVEVC HygR KARPRTSPRRNGSDALVEVC c t t t g c c c t c g g a c g a g t g c t g g g g c g t c g g t t t c c a c t a t c g g c g a g t a c t t c t a c a c a g a a a c g g g a g c c t g c t c a c g a c c c c g c a g c c a a a g g t g a t a g c c g c t c a t g a a g a t g t g t

2040 320 315 310 305 GDTWVAASRRAIQTRGVTGA HygR 320 315 310 305 GDTWVAASRRAIQTRGVTGA HygR GDTWVAASRRAIQTRGVTGA g c c a t c g g t c c a g a c g g c c g c g c t t c t g c g g g c g a t t t g t g t a c g c c c g a c a g t c c c g g c c g g t a g c c a g g t c t g c c g g c g c g a a g a c g c c c g c t a a a c a c a t g c g g g c t g t c a g g g c c g

2100 300 295 290 285 GSRVIADCRGQAWAADDFNG HygR 300 295 290 285 GSRVIADCRGQAWAADDFNG HygR GSRVIADCRGQAWAADDFNG t c c g g a t c g g a c g a t t g c g t c g c a t c g a c c c t g c g c c c a a g c t g c a t c a t c g a a a t t g c c a g g c c t a g c c t g c t a a c g c a g c g t a g c t g g g a c g c g g g t t c g a c g t a g t a g c t t t a a c g g

2160 280 275 270 265 DVLSQYLQDLGIRLMYARLR HygR 280 275 270 265 DVLSQYLQDLGIRLMYARLR HygR DVLSQYLQDLGIRLMYARLR g t c a a c c a a g c t c t g a t a g a g t t g g t c a a g a c c a a t g c g g a g c a t a t a c g c c c g g a g t c g c a g t t g g t t c g a g a c t a t c t c a a c c a g t t c t g g t t a c g c c t c g t a t a t g c g g g c c t c a g c

2220 260 255 250 245 PSGALEPHRREFYRTQQEMC HygR 260 255 250 245 PSGALEPHRREFYRTQQEMC HygR MPPLEVARLLLHT PSGALEPHRREFYRTQQEMC t g g c g a t c c t g c a a g c t c c g g a t g c c t c c g c t c g a a g t a g c g c g t c t g c t g c t c c a t a c a a c c g c t a g g a c g t t c g a g g c c t a c g g a g g c g a g c t t c a t c g c g c a g a c g a c g a g g t a t g t

2280 240 235 230 225 ALWPRWFFINAVEYQSDGFM HygR 240 235 230 225 ALWPRWFFINAVEYQSDGFM HygR SQPRPPEEDVGDLVLGIPEH ALWPRWFFINAVEYQSDGFM a g c c a a c c a c g g c c t c c a g a a g a a g a t g t t g g c g a c c t c g t a t t g g g a a t c c c c g a a c a t t c g g t t g g t g c c g g a g g t c t t c t t c t a c a a c c g c t g g a g c a t a a c c c t t a g g g g c t t g t a

2340 220 215 210 205 AESWDIVATIRGNDTLVNNS HygR 220 215 210 205 AESWDIVATIRGNDTLVNNS HygR RLAPVNDRCYAAIVRQDIVG AESWDIVATIRGNDTLVNNS c g c c t c g c t c c a g t c a a t g a c c g c t g t t a t g c g g c c a t t g t c c g t c a g g a c a t t g t t g g a g c g g a g c g a g g t c a g t t a c t g g c g a c a a t a c g c c g g t a a c a g g c a g t c c t g t a a c a a c c t

2400 200 195 190 185 GFDAHVLHRVEPCDEAWLML HygR 200 195 190 185 GFDAHVLHRVEPCDEAWLML HygR AEIRVHEVPDFGAVLGPKHQ GFDAHVLHRVEPCDEAWLML g c c g a a a t c c g c g t g c a c g a g g t g c c g g a c t t c g g g g c a g t c c t c g g c c c a a a g c a t c a g c g g c t t t a g g c g c a c g t g c t c c a c g g c c t g a a g c c c c g t c a g g a g c c g g g t t t c g t a g t c

2460 180 175 170 165 EDLAQAVSASVTDDMVTQWH HygR 180 175 170 165 EDLAQAVSASVTDDMVTQWH HygR LIESLRDGRTDGVVHHSLPV EDLAQAVSASVTDDMVTQWH c t c a t c g a g a g c c t g c g c g a c g g a c g c a c t g a c g g t g t c g t c c a t c a c a g t t t g c c a g t g g a g t a g c t c t c g g a c g c g c t g c c t g c g t g a c t g c c a c a g c a g g t a g t g t c a a a c g g t c a c

2520 160 155 150 145 YVHPDAIACIFDRWTTYQGI HygR 160 155 150 145 YVHPDAIACIFDRWTTYQGI HygR IHMGISNRAYEITPCSVLTD YVHPDAIACIFDRWTTYQGI a t a c a c a t g g g g a t c a g c a a t c g c g c a t a t g a a a t c a c g c c a t g t a g t g t a t t g a c c g a t t a t g t g t a c c c c t a g t c g t t a g c g c g t a t a c t t t a g t g c g g t a c a t c a c a t a a c t g g c t a

AsiSIRsrII 2580 140 135 130 125 GQPGFPGFGSTQSLDAAAIA HygR 140 135 130 125 GQPGFPGFGSTQSLDAAAIA HygR SLRSEWAEPARLAKIGRSDR GQPGFPGFGSTQSLDAAAIA t c c t t g c g g t c c g a a t g g g c c g a a c c c g c t c g t c t g g c t a a g a t c g g c c g c a g c g a t c g c a g g a a c g c c a g g c t t a c c c g g c t t g g g c g a g c a g a c c g a t t c t a g c c g g c g t c g c t a g c g

2640 120 115 110 105 DMAEAVPQLVAPLETEPLDQ HygR 120 115 110 105 DMAEAVPQLVAPLETEPLDQ HygR IHGLRDRLQNSGQFGFRQVL DMAEAVPQLVAPLETEPLDQ a t c c a t g g c c t c c g c g a c c g g c t g c a g a a c a g c g g g c a g t t c g g t t t c a g g c a g g t c t t g t a g g t a c c g g a g g c g c t g g c c g a c g t c t t g t c g c c c g t c a a g c c a a a g t c c g t c c a g a a c

2700 100 95 90 85 LTVGQARRSICYTLSESFEG HygR 100 95 90 85 LTVGQARRSICYTLSESFEG HygR QRDTLCTAGDAIGQALAEFP LTVGQARRSICYTLSESFEG c a a c g t g a c a c c c t g t g c a c g g c g g g a g a t g c a a t a g g t c a g g c t c t c g c t g a a t t c c c c g t t g c a c t g t g g g a c a c g t g c c g c c c t c t a c g t t a t c c a g t c c g a g a g c g a c t t a a g g g g

2760 80 75 70 65 IDLVEPIPLAASAFHRYVYR HygR 80 75 70 65 IDLVEPIPLAASAFHRYVYR HygR NVKHFRNRERGRCKVPINIT IDLVEPIPLAASAFHRYVYR a a t g t c a a g c a c t t c c g g a a t c g g g a g c g c g g c c g a t g c a a a g t g c c g a t a a a c a t a a c g t t a c a g t t c g t g a a g g c c t t a g c c c t c g c g c c g g c t a c g t t t c a c g g c t a t t t g t a t t g c

2820 60 55 50 45 DKYFGDACSNVRLVYGRGGV HygR 60 55 50 45 DKYFGDACSNVRLVYGRGGV HygR IFVETIGAAIYPQDISTPSY DKYFGDACSNVRLVYGRGGV a t c t t t g t a g a a a c c a t c g g c g c a g c t a t t t a c c c g c a g g a c a t a t c c a c g c c c t c c t a c t a g a a a c a t c t t t g g t a g c c g c g t c g a t a a a t g g g c g t c c t g t a t a g g t g c g g g a g g a t g

AhdI 2880 40 35 30 25 DFSFARSEEGESLQMLDSVS HygR 40 35 30 25 DFSFARSEEGESLQMLDSVS HygR IEAESTRFFALRELHQVGHA DFSFARSEEGESLQMLDSVS a t c g a a g c t g a a a g c a c g a g a t t c t t c g c c c t c c g a g a g c t g c a t c a g g t c g g a c a c g c t t a g c t t c g a c t t t c g t g c t c t a a g a a g c g g g a g g c t c t c g a c g t a g t c c a g c c t g t g c g a

2940 20 15 10 5 1 DFKEILFKEVSTATLEPKKM HygR 20 15 10 5 1 DFKEILFKEVSTATLEPKKM HygR VELFDQKLLDRRRGEFRLFH DFKEILFKEVSTATLEPKKM g t c g a a c t t t t c g a t c a g a a a c t t c t c g a c a g a c g t c g c g g t g a g t t c a g g c t t t t t c a t c a g c t t g a a a a g c t a g t c t t t g a a g a g c t g t c t g c a g c g c c a c t c a a g t c c g a a a a a g t a

3000 ISLPPGSAKARER* a t c t c a t t g c c c c c c g g a t c t g c g a a a g c t c g a g a g a g a t a g a t t t g t a g a g a g a g a c t g t a g a g t a a c g g g g g g c c t a g a c g c t t t c g a g c t c t c t c t a t c t a a a c a t c t c t c t c t g a c

3060 CaMV 35S promoter (enhanced) C T T C C C A G A A C 35S promoter g t g a t t t c a g c g t g t c c t c t c c a a a t g a a a t g a a c t t c c t t a t a t a g a g g a a g g g t c t t g c a c t a a a g t c g c a c a g g a g a g g t t t a c t t t a c t t g a a g g a a t a t a t c t c c t t c c c a g a a c

3120 CaMV 35S promoter (enhanced) G C T T C C T A T C 35S promoter c g a a g g a t a g t g g g a t t g t g c g t c a t c c c t t a c g t c a g t g g a g a t a t c a c a t c a a t c c a c g c t t c c t a t c a c c c t a a c a c g c a g t a g g g a a t g c a g t c a c c t c t a t a g t g t a g t t a g g t g

3180 CaMV 35S promoter (enhanced) t t g c t t t g a a g a c g t g g t t g g a a c g t c t t c t t t t t c c a c g a t g c t c c t c g t g g g t g g g g g a a c g a a a c t t c t g c a c c a a c c t t g c a g a a g a a a a a g g t g c t a c g a g g a g c a c c c a c c c c c

3240 CaMV 35S promoter (enhanced) t c c a t c t t t g g g a c c a c t g t c g g c a g a g g c a t c t t g a a c g a t a g c c t t t c c t t t a t c g c a a g g t a g a a a c c c t g g t g a c a g c c g t c t c c g t a g a a c t t g c t a t c g g a a a g g a a a t a g c g t

3300 CaMV 35S promoter (enhanced) a t g a t g g c a t t t g t a g g t g c c a c c t t c c t t t t c t a c t g t c c t t t t g a t g a a g t g a c a g a t t a c t a c c g t a a a c a t c c a c g g t g g a a g g a a a a g a t g a c a g g a a a a c t a c t t c a c t g t c t a

3360 CaMV 35S promoter (enhanced) a g c t g g g c a a t g g a a t c c g a g g a g g t t t c c c g a t a t t a c c c t t t g t t g a a a a g t c t c a a t t c g a c c c g t t a c c t t a g g c t c c t c c a a a g g g c t a t a a t g g g a a a c a a c t t t t c a g a g t t a

3420 CaMV 35S promoter (enhanced) a g c c c t t t g g t c t t c t g a g a c t g t a t c t t t g a t a t t c t t g g a g t a g a c g a g a g t g t c g t g t c g g g a a a c c a g a a g a c t c t g a c a t a g a a a c t a t a a g a a c c t c a t c t g c t c t c a c a g c a c

3480 CaMV 35S promoter (enhanced) *DVQKSSTTPVDEKE c t c c a c c a t g t t c a c a t c a a t c c a c t t g c t t t g a a g a c g t g g t t g g a a c g t c t t c t t t t t g a g g t g g t a c a a g t g t a g t t a g g t g a a c g a a a c t t c t g c a c c a a c c t t g c a g a a g a a a a a

3540 CaMV 35S promoter (enhanced) VISRTPPPGDKPGSDASADQ c c a c g a t g c t c c t c g t g g g t g g g g g t c c a t c t t t g g g a c c a c t g t c g g c a g a g g c a t c t t g g t g c t a c g a g g a g c a c c c a c c c c c a g g t a g a a a c c c t g g t g a c a g c c g t c t c c g t a g a a

3600 CaMV 35S promoter (enhanced) VIAKGKDCHHCKYTGGEKEV g a a c g a t a g c c t t t c c t t t a t c g c a a t g a t g g c a t t t g t a g g t g c c a c c t t c c t t t t c t a c t t g c t a t c g g a a a g g a a a t a g c g t t a c t a c c g t a a a c a t c c a c g g t g g a a g g a a a a g a t

3660 CaMV 35S promoter (enhanced) TRKIFHCIAPCHFGLLNGSI c t g t c c t t t t g a t g a a g t g a c a g a t a g c t g g g c a a t g g a a t c c g a g g a g g t t t c c c g a t a g a c a g g a a a a c t a c t t c a c t g t c t a t c g a c c c g t t a c c t t a g g c t c c t c c a a a g g g c t a t

3720 CaMV 35S promoter (enhanced) VRQQFTEIARQDESVTDKIN t t a c c c t t t g t t g a a a a g t c t c a a t a g c c c t t t g g t c t t c t g a g a c t g t a t c t t t g a t a t a a t g g g a a a c a a c t t t t c a g a g t t a t c g g g a a a c c a g a a g a c t c t g a c a t a g a a a c t a t a

3780 KSYVLTDHEVMNALQELWYA t c t t g g a g t a g a c g a g a g t g t c g t g c t c c a c c a t g t t g g c a a g c t g c t c t a g c c a a t a c g a g a a c c t c a t c t g c t c t c a c a g c a c g a g g t g g t a c a a c c g t t c g a c g a g a t c g g t t a t g c

3840 FRREGRTPRNMLAAPVVPKG c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g

3900 CAP binding site VPFRATLAVCNIHTLESM c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g

3960 -35 -10 lac promoter lac operator A G C G G A T A M13/pUC Reverse a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t

4020 lac operator M13 rev A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse a c a a t t t c a c a c a g g a a a c a g c t a t g a c a t g a t t a c g a a t t c g a g t a a g g t a g t g a a c a g t g t t a a a g t g t g t c c t t t g t c g a t a c t g t a c t a a t g c t t a a g c t c a t t c c a t c a c t t g t c

4080 a a g t c c g g c a g g t c c t t a g c g a a a a a a c g g g g t g t g c c a g a a a a c t c t a t c c t c t a c c c t t t c a g g c c g t c c a g g a a t c g c t t t t t t g c c c c a c a c g g t c t t t t g a g a t a g g a g a t g g g a

4140 g c g t g g a g g t g t g a a t t c t g c a c a c t g c a a a t g c a a t g t g t c c a a t g c t t t a t a t a g g g c c g c a c c t c c a c a c t t a a g a c g t g t g a c g t t t a c g t t a c a c a g g t t a c g a a a t a t a t c c c g

4200 a g g t t t t g g c g g g a g a a c a g g g c c c t a g t g t t c c c a c g g t a g c g t a g c g a a t c g t g t g g g t c c a a a a c c g c c c t c t t g t c c c g g g a t c a c a a g g g t g c c a t c g c a t c g c t t a g c a c a c c c

4260 c c c t g t t c g g t g t g c g g t c g g g g g g c c t c c a c g c g g g t t a t a a t a t t a c c c c g c g t g g t g g g g a c a a g c c a c a c g c c a g c c c c c c g g a g g t g c g c c c a a t a t t a t a a t g g g g c g c a c c a c

4320 g c c c c c g a c g c g c a c t c g g c t t t t c g t g a g t g c g c g g a g g c t t t t g g a c c a c a t c t t t t c c g g g g g c t g c g c g t g a g c c g a a a a g c a c t c a c g c g c c t c c g a a a a c c t g g t g t a g a a a a g

4380 t g a t c a c t t t c g t g g a a g a t g t t g a t t t a t c a c a c t t t t g a c g g g g a a a t c t g t g c c a t g a c t a g t g a a a g c a c c t t c t a c a a c t a a a t a g t g t g a a a a c t g c c c c t t t a g a c a c g g t a c

4440 c c t t a g c t t a t a a g g a a g t g c g t g g t a g c c c a t c t c g a c a a g t t t g t a c c g a t c a a a a g c g g a a t c g a a t a t t c c t t c a c g c a c c a t c g g g t a g a g c t g t t c a a a c a t g g c t a g t t t t c g

4500 attR1 a g g t g a c a a g t t t g t a c a a a a a a g c t g a a c g a g a a a c g t a a a a t g a t a t a a a t a t c a a t a t c c a c t g t t c a a a c a t g t t t t t t c g a c t t g c t c t t t g c a t t t t a c t a t a t t t a t a g t t a t

4560 attR1 t a t t a a a t t a g a t t t t g c a t a a a a a a c a g a c t a c a t a a t a c t g t a a a a c a c a a c a t a t c c a t a a t t t a a t c t a a a a c g t a t t t t t t g t c t g a t g t a t t a t g a c a t t t t g t g t t g t a t a g g

4620 -35 attR1 lac UV5 promoter a g t c a c t a t g g c g g c c g c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t c a g t g a t a c c g c c g g c g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t

4680 -10 1 M lac UV5 promoter CmR M t a a t g t g t g g a t t t t g a g t t a g g a t c c g t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t a t t a c a c a c c t a a a a c t c a a t c c t a g g c a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a

4740 5 10 15 20 EKKITGYTTVDISQWHRKEH CmR EKKITGYTTVDISQWHRKEH g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t

4800 25 30 35 40 FEAFQSVAQCTYNQTVQLDI CmR FEAFQSVAQCTYNQTVQLDI t t t t g a g g c a t t t c a g t c a g t c g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t a a a a c t c c g t a a a g t c a g t c a g c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a

4860 45 50 55 60 TAFLKTVKKNKHKFYPAFIH CmR TAFLKTVKKNKHKFYPAFIH t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t

4920 65 70 75 80 ILARLMNAHPEFRMAMKDGE CmR ILARLMNAHPEFRMAMKDGE c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a c g g t g a g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t g c c a c t

4980 85 90 95 100 LVIWDSVHPCYTVFHEQTET CmR LVIWDSVHPCYTVFHEQTET g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g

5040 105 110 115 120 FSSLWSEYHDDFRQFLHIYS CmR FSSLWSEYHDDFRQFLHIYS g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g

5100 125 130 135 140 QDVACYGENLAYFPKGFIEN CmR QDVACYGENLAYFPKGFIEN g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t

5160 145 150 155 160 MFFVSANPWVSFTSFDLNVA CmR MFFVSANPWVSFTSFDLNVA t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g

5220 165 170 175 180 NMDNFFAPVFTMGKYYTQGD CmR NMDNFFAPVFTMGKYYTQGD c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t

5280 185 190 195 200 KVLMPLAIQVHHAVCDGFHV CmR KVLMPLAIQVHHAVCDGFHV c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a

XbaI 5340 205 210 215 220 GRMLNELQQYCDEWQAGRNL CmR GRMLNELQQYCDEWQAGRNL c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g c g g g g c g t a a t c t g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c g c c c c g c a t t a g a

BssHII 5400 225 EDPAY* CmR EDPAY* a g a g g a t c c g g c t t a c t a a a a g c c a g a t a a c a g t a t g c g t a t t t g c g c g c t g a t t t t t g c t c t c c t a g g c c g a a t g a t t t t c g g t c t a t t g t c a t a c g c a t a a a c g c g c g a c t a a a a a c g

5460 g g t a t a a g a a t a t a t a c t g a t a t g t a t a c c c g a a g t a t g t c a a a a a g a g g t a t g c t a t g a c c a t a t t c t t a t a t a t g a c t a t a c a t a t g g g c t t c a t a c a g t t t t t c t c c a t a c g a t a c t

5520 a g c a g c g t a t t a c a g t g a c a g t t g a c a g c g a c a g c t a t c a g t t g c t c a a g g c a t a t a t g a t c g t c g c a t a a t g t c a c t g t c a a c t g t c g c t g t c g a t a g t c a a c g a g t t c c g t a t a t a c t

5580 t g t c a a t a t c t c c g g t c t g g t a a g c a c a a c c a t g c a g a a t g a a g c c c g t c g t c t g c g t g c a c a g t t a t a g a g g c c a g a c c a t t c g t g t t g g t a c g t c t t a c t t c g g g c a g c a g a c g c a c g

5640 c g a a c g c t g g a a a g c g g a a a a t c a g g a a g g g a t g g c t g a g g t c g c c c g g t t t a t t g a a a t g c t t g c g a c c t t t c g c c t t t t a g t c c t t c c c t a c c g a c t c c a g c g g g c c a a a t a a c t t t a

5700 1 5 MQFKVYT ccdB g a a c g g c t c t t t t g c t g a c g a g a a c a g g g g c t g g t g a a a t g c a g t t t a a g g t t t a c a c c t c t t g c c g a g a a a a c g a c t g c t c t t g t c c c c g a c c a c t t t a c g t c a a a t t c c a a a t g t g g a

5760 10 15 20 25 YKRESRYRLFVDVQSDIIDT ccdB a t a a a a g a g a g a g c c g t t a t c g t c t g t t t g t g g a t g t a c a g a g t g a t a t t a t t g a c a c g c t a t t t t c t c t c t c g g c a a t a g c a g a c a a a c a c c t a c a t g t c t c a c t a t a a t a a c t g t g c g

TspMIXmaISmaISrfI 5820 30 35 40 45 PGRRMVIPLASARLLSDKVP ccdB c c g g g c g a c g g a t g g t g a t c c c c c t g g c c a g t g c a c g t c t g c t g t c a g a t a a a g t c c c c c g g c c c g c t g c c t a c c a c t a g g g g g a c c g g t c a c g t g c a g a c g a c a g t c t a t t t c a g g g g g

5880 50 55 60 65 RELYPVVHIGDESWRMMTTD ccdB G C A T G A T G A C C A C C G A T A ccdB-fwd g t g a a c t t t a c c c g g t g g t g c a t a t c g g g g a t g a a a g c t g g c g c a t g a t g a c c a c c g a t a c a c t t g a a a t g g g c c a c c a c g t a t a g c c c c t a c t t t c g a c c g c g t a c t a c t g g t g g c t a t

5940 70 75 80 85 MASVPVSVIGEEVADLSHRE ccdB T G ccdB-fwd t g g c c a g t g t g c c g g t c t c c g t t a t c g g g g a a g a a g t g g c t g a t c t c a g c c a c c g c g a a a a c c g g t c a c a c g g c c a g a g g c a a t a g c c c c t t c t t c a c c g a c t a g a g t c g g t g g c g c t t t

6000 90 95 100 NDIKNAINLMFWGI* ccdB a t g a c a t c a a a a a c g c c a t t a a c c t g a t g t t c t g g g g a a t a t a a a t g t c a g g c t c c c t t a t a c t g t a g t t t t t g c g g t a a t t g g a c t a c a a g a c c c c t t a t a t t t a c a g t c c g a g g g a a t

SalI 6060 attR2 t a c a c a g c c a g t c t g c a g g t c g a c c a t a g t g a c t g g a t a t g t t g t g t t t t a c a g t a t t a t a t g t g t c g g t c a g a c g t c c a g c t g g t a t c a c t g a c c t a t a c a a c a c a a a a t g t c a t a a t a

6120 attR2 g t a g t c t g t t t t t t a t g c a a a a t c t a a t t t a a t a t a t t g a t a t t t a t a t c a t t t t a c g t t c a t c a g a c a a a a a a t a c g t t t t a g a t t a a a t t a t a t a a c t a t a a a t a t a g t a a a a t g c a a

BstEII 6180 attR2 t c t c g t t c a g c t t t c t t g t a c a a a g t g g t c a c c t g c a a a a a g t g t t t g a t c g c c g g c g g t a g a g c a a g t c g a a a g a a c a t g t t t c a c c a g t g g a c g t t t t t c a c a a a c t a g c g g c c g c c a

6240 a c a a a g t g g t t a a a a t a a t a t t t t a t t t a t c t c a t g t c a t t c g a t t a c a g a g g c t c g g c t t g t t t c a c c a a t t t t a t t a t a a a a t a a a t a g a g t a c a g t a a g c t a a t g t c t c c g a g c c g a

6300 a c g a g c a a a g a c a a a c c a a a t a t a a c a a a c a a c a a c c c t t a c a c a a t g a c a t c g g a a a a c t g c t c g t t t c t g t t t g g t t t a t a t t g t t t g t t g t t g g g a a t g t g t t a c t g t a g c c t t t t g

6360 g a a a t a c a a c a c c c t g a g a t a t t a c a t t t a t a g a a a c t g t a c g c c g t c c g c g c t a g g a c a c t t t a t g t t g t g g g a c t c t a t a a t g t a a a t a t c t t t g a c a t g c g g c a g g c g c g a t c c t g t

6420 g t c a c t g c g a a g c a g t g a c g t c t t c g c c g g a g g c g a a c g a g t a g t t g a t g a a c g t c t c g c c a g t g a c g c t t c g t c a c t g c a g a a g c g g c c t c c g c t t g c t c a t c a a c t a c t t g c a g a g c g

6480 c t t c a t a c a t g t a g t g a a c a a c a g t g t t a g a g t a c a t g t a a t c c g a c t g t t c g g g a g t c a g a a g t a t g t a c a t c a c t t g t t g t c a c a a t c t c a t g t a c a t t a g g c t g a c a a g c c c t c a g t

6540 t a t c c t t g a g c c a a t c t t c g t c t g g a t t a a c t a a a a t g a t g c a a g g t a t t c c a c c c c g t a a t a g g a a c t c g g t t a g a a g c a g a c c t a a t t g a t t t t a c t a c g t t c c a t a a g g t g g g g c a t

6600 t g a c c t t t c g c t t a c c a t a t t t t g g a t t g a c c g t g a a g t c a c g c t g a g c c c c g a c g a a g c a c t g g a a a g c g a a t g g t a t a a a a c c t a a c t g g c a c t t c a g t g c g a c t c g g g g c t g c t t c g

6660 a c t t c c a g t t g g g t g t g a a c t t g a a t g g a a t g t c g t c g a t g a t a t t a t a c t t g g c g t t g a t g a a g g t c a a c c c a c a c t t g a a c t t a c c t t a c a g c a g c t a c t a t a a t a t g a a c c g c a a c t

6720 *LGQ c g t c a t a t g t t g t g a a a t c a a c t a g a c t g t t a t a a t a a t t g t g t g t c c c t a g a g a c c t t g g c a g t a t a c a a c a c t t t a g t t g a t c t g a c a a t a t t a t t a a c a c a c a g g g a t c t c t g g a a c

6780 GPLREQEPQGASTSPSIGGR c c c a g g a a g t c t t t c c t g t t c t g g t t g g c c c g c a g a t g t a g a t g g a c t t a t g c c t c c c c g g g g t c c t t c a g a a a g g a c a a g a c c a a c c g g g c g t c t a c a t c t a c c t g a a t a c g g a g g g g c

6840 HSRSYDDMWELDSQAQDAPT g t g a c t c c t g g a a t a a t c g t c c a t c c a c t c t a a g t c a g a t t g c g c t t g a t c c g c a g g a g t c a c t g a g g a c c t t a t t a g c a g g t a g g t g a g a t t c a g t c t a a c g c g a a c t a g g c g t c c t c a

6900 STCLIYSELSVSYLHENKWS g g a a g t a c a a a g g a t a t a g g a t t c g a g g c t t a c g g a g t a g a g a t g t t c a t t t t t c c a g c t c c t t c a t g t t t c c t a t a t c c t a a g c t c c g a a t g c c t c a t c t c t a c a a g t a a a a a g g t c g a

6960 EITEHCILSETPFEPTYTAV t t c a a t g g t c t c a t g g c a a a t g a g t g a t t c g g t t g g a a a c t c a g g t g t g t a a g t g g c a a c a a g t t a c c a g a g t a c c g t t t a c t c a c t a a g c c a a c c t t t g a g t c c a c a c a t t c a c c g t t g

7020 PDPFLHRATYEFDKLRISWE t g g g t c a g g a a a t a g a t g g c g t g c c g t g t a c t c g a a g t c t t t g a g a c g g a t a g a c c a t t c a c c c a g t c c t t t a t c t a c c g c a c g g c a c a t g a g c t t c a g a a a c t c t g c c t a t c t g g t a a g

NruI 7080 FPFRNCVMSLFEERSSSSEI a a a c g g a a a a c g a t t g c a a a c c a t g c t g a g g a a t t c c t c g c g a g a g g a a c t a g a t t c a a t t t t g c c t t t t g c t a a c g t t t g g t a c g a c t c c t t a a g g a g c g c t c t c c t t g a t c t a a g t t a

7140 IQKMDADRDKRGPTSVAVFT g a t c t g t t t c a t a t c c g c a t c a c g g t c t t t a c g a c c t g g a g t t g a a a c a g c c a c g a a t g t c t a g a c a a a g t a t a g g c g t a g t g c c a g a a a t g c t g g a c c t c a a c t t t g t c g g t g c t t a c a

7200 GWEATNVDSDVEKTIYDRVQ t c c c c a c t c a g c t g t g t t t a c a t c g g a g t c a a c c t c c t t c g t g a t g t a a t c a c g a a c t t g a g g g g t g a g t c g a c a c a a a t g t a g c c t c a g t t g g a g g a a g c a c t a c a t t a g t g c t t g a a c

7260 NCDKAAQINPHFISFPSTDM g t t g c a g t c t t t g g c a g c t t g t a t a t t t g g a t g g a a t a t g g a g a a t g g a g a t g t a t c c a t c a a c g t c a g a a a c c g t c g a a c a t a t a a a c c t a c c t t a t a c c t c t t a c c t c t a c a t a g g t a

HindIII 7320 RLNLANPNTISARLKNQVLV a c g g a g g t t t a a g g c a t t g g g a t t g g t g a t g g a a g c a c g a a g c t t g t t c t g c a c g a g a a c t g c c t c c a a a t t c c g t a a c c c t a a c c a c t a c c t t c g t g c t t c g a a c a a g a c g t g c t c t t g

Eco53kISacI 7380 HLHPSGDEHLERVAAIYLPE g t g c a g a t g t g g t g a t c c a t c t t c g t g g a g c t c t c t a a c a g c a g c g a t g t a g a g g g g c t c c a c g t c t a c a c c a c t a g g t a g a a g c a c c t c g a g a g a t t g t c g t c g c t a c a t c t c c c c g a g

7440 YKNLLTRLSDLAYQPELTCQ a t a t t t g t t c a a g a g a g t g c g a a g t g a a t c c a a g g c g t a c t g t g g c t c a a g g g t a c a t t g t a t a a a c a a g t t c t c t c a c g c t t c a c t t a g g t t c c g c a t g a c a c c g a g t t c c c a t g t a a c

7500 PYTLFLYKSYVRFRPASSSA a g g a t a t g t t a g a a a g a g g t a c t t g g a a t a g a c a c g g a a c c t g g g t g c a g a t g a a g a g g c t c c t a t a c a a t c t t t c t c c a t g a a c c t t a t c t g t g c c t t g g a c c c a c g t c t a c t t c t c c g

7560 M c a t g g t a g t g a a c a g a a g t c c g g c a g g t c c t t a g c g a a a a a a c g g g g t g t g c c a g a a a a c g t a c c a t c a c t t g t c t t c a g g c c g t c c a g g a a t c g c t t t t t t g c c c c a c a c g g t c t t t t g

7620 t c t a t c c t c t a c c c t g c g t g g a g g t g t g a a t t c t g c a c a c t g c a a a t g c a a t g t g t c c a a a g a t a g g a g a t g g g a c g c a c c t c c a c a c t t a a g a c g t g t g a c g t t t a c g t t a c a c a g g t t

7680 t g c t t t a t a t a g g g c a g g t t t t g g c g g g a g a a c a g g g c c c t a g t g t t c c c a c g g t a g c g t a c g a a a t a t a t c c c g t c c a a a a c c g c c c t c t t g t c c c g g g a t c a c a a g g g t g c c a t c g c a

7740 a g c g a a t c g t g t g g g c c c t g t t c g g t g t g c g g t c g g g g g g c c t c c a c g c g g g t t a t a a t a t c g c t t a g c a c a c c c g g g a c a a g c c a c a c g c c a g c c c c c c g g a g g t g c g c c c a a t a t t a t

7800 t t a c c c c g c g t g g t g g c c c c c g a c g c g c a c t c g g c t t t t c g t g a g t g c g c g g a g g c t t t t a a t g g g g c g c a c c a c c g g g g g c t g c g c g t g a g c c g a a a a g c a c t c a c g c g c c t c c g a a a a

7860 g g a c c a c a t c t t t t c t g a t c a c t t t c g t g g a a g a t g t t g a t t t a t c a c a c t t t t g a c g g g c c t g g t g t a g a a a a g a c t a g t g a a a g c a c c t t c t a c a a c t a a a t a g t g t g a a a a c t g c c c

7920 g a a a t c t g t g c c a t g c c t t a g c t t a t a a g g a a g t g c g t g g t a g c c c a t c t c g g g g c c c t c c t t t a g a c a c g g t a c g g a a t c g a a t a t t c c t t c a c g c a c c a t c g g g t a g a g c c c c g g g a g

PmeI 7980 RB T-DNA repeat g a t t c g a c g t t c c t g t t t a a a c t a t c a g t g t t t g a c a g g a t a t a t t g g c g g g t a a a c c t a c t a a g c t g c a a g g a c a a a t t t g a t a g t c a c a a a c t g t c c t a t a t a a c c g c c c a t t t g g a t

8040 a g a g a a a a g a g c g t t t a t t a g a a t a a t c g g a t a t t t a a a a g g g c g t g a a a a g g t t t a t c c t c t c t t t t c t c g c a a a t a a t c t t a t t a g c c t a t a a a t t t t c c c g c a c t t t t c c a a a t a g g

SphI 8100 g t t c g t c c a t t t g t a t g t g c a t g c c a a c c a c a g g g t t c c c c t c g g g a t c a a a g t a c t t t g c a a g c a g g t a a a c a t a c a c g t a c g g t t g g t g t c c c a a g g g g a g c c c t a g t t t c a t g a a a c

8160 a t c c a a c c c c t c c g c t g c t a t a g t g c a g t c g g c t t c t g a c g t t c a g t g c a g c c g t c t t c t t a g g t t g g g g a g g c g a c g a t a t c a c g t c a g c c g a a g a c t g c a a g t c a c g t c g g c a g a a g a

8220 g a a a a c g a c a t g t c g c a c a a g t c c t a a g t t a c g c g a c a g g c t g c c g c c c t g c c c t t t t c c c t t t t g c t g t a c a g c g t g t t c a g g a t t c a a t g c g c t g t c c g a c g g c g g g a c g g g a a a a g g

8280 t g g c g t t t t c t t g t c g c g t g t t t t a g t c g c a t a a a g t a g a a t a c t t g c g a c t a g a a c c g g a c c g c a a a a g a a c a g c g c a c a a a a t c a g c g t a t t t c a t c t t a t g a a c g c t g a t c t t g g c c

8340 MNKSAAAGLLGYARVS a g a c a t t a c g c c a t g a a c a a g a g c g c c g c c g c t g g c c t g c t g g g c t a t g c c c g c g t c a g c t c t g t a a t g c g g t a c t t g t t c t c g c g g c g g c g a c c g g a c g a c c c g a t a c g g g c g c a g t c g

8400 TDDQDLTNQRAELHAAGCTK a c c g a c g a c c a g g a c t t g a c c a a c c a a c g g g c c g a a c t g c a c g c g g c c g g c t g c a c c a a g t g g c t g c t g g t c c t g a a c t g g t t g g t t g c c c g g c t t g a c g t g c g c c g g c c g a c g t g g t t c

8460 LFSEKITGTRRDRPELARML c t g t t t t c c g a g a a g a t c a c c g g c a c c a g g c g c g a c c g c c c g g a g c t g g c c a g g a t g c t t g a c a a a a g g c t c t t c t a g t g g c c g t g g t c c g c g c t g g c g g g c c t c g a c c g g t c c t a c g a a

8520 DHLRPGDVVTVTRLDRLARS g a c c a c c t a c g c c c t g g c g a c g t t g t g a c a g t g a c c a g g c t a g a c c g c c t g g c c c g c a g c c t g g t g g a t g c g g g a c c g c t g c a a c a c t g t c a c t g g t c c g a t c t g g c g g a c c g g g c g t c g

8580 TRDLLDIAERIQEAGAGLRS a c c c g c g a c c t a c t g g a c a t t g c c g a g c g c a t c c a g g a g g c c g g c g c g g g c c t g c g t a g c t g g g c g c t g g a t g a c c t g t a a c g g c t c g c g t a g g t c c t c c g g c c g c g c c c g g a c g c a t c g

8640 LAEPWADTTTPAGRMVLTVF c t g g c a g a g c c g t g g g c c g a c a c c a c c a c g c c g g c c g g c c g c a t g g t g t t g a c c g t g t t c g a c c g t c t c g g c a c c c g g c t g t g g t g g t g c g g c c g g c c g g c g t a c c a c a a c t g g c a c a a g

8700 AGIAEFERSLIIDRTRSGRE g c c g g c a t t g c c g a g t t c g a g c g t t c c c t a a t c a t c g a c c g c a c c c g g a g c g g g c g c g a g c g g c c g t a a c g g c t c a a g c t c g c a a g g g a t t a g t a g c t g g c g t g g g c c t c g c c c g c g c t c

8760 AAKARGVKFGPRPTLTPAQI g c c g c c a a g g c c c g a g g c g t g a a g t t t g g c c c c c g c c c t a c c c t c a c c c c g g c a c a g a t c c g g c g g t t c c g g g c t c c g c a c t t c a a a c c g g g g g c g g g a t g g g a g t g g g g c c g t g t c t a g

8820 AHARELIDQEGRTVKEAAAL g c g c a c g c c c g c g a g c t g a t c g a c c a g g a a g g c c g c a c c g t g a a a g a g g c g g c t g c a c t g c g c g t g c g g g c g c t c g a c t a g c t g g t c c t t c c g g c g t g g c a c t t t c t c c g c c g a c g t g a c

8880 LGVHRSTLYRALERSEEVTP c t t g g c g t g c a t c g c t c g a c c c t g t a c c g c g c a c t t g a g c g c a g c g a g g a a g t g a c g c c c g a a c c g c a c g t a g c g a g c t g g g a c a t g g c g c g t g a a c t c g c g t c g c t c c t t c a c t g c g g g

8940 TEARRRGAFREDALTEADAL a c c g a g g c c a g g c g g c g c g g t g c c t t c c g t g a g g a c g c a t t g a c c g a g g c c g a c g c c c t g t g g c t c c g g t c c g c c g c g c c a c g g a a g g c a c t c c t g c g t a a c t g g c t c c g g c t g c g g g a c

9000 AAAENERQEEQA* g c g g c c g c c g a g a a t g a a c g c c a a g a g g a a c a a g c a t g a a a c c g c a c c a g g a c g g c c a g g c g c c g g c g g c t c t t a c t t g c g g t t c t c c t t g t t c g t a c t t t g g c g t g g t c c t g c c g g t c c

9060 a c g a a c c g t t t t t c a t t a c c g a a g a g a t c g a g g c g g a g a t g a t c g c g g c c g g g t a c g t g t t g c t t g g c a a a a a g t a a t g g c t t c t c t a g c t c c g c c t c t a c t a g c g c c g g c c c a t g c a c a

9120 t c g a g c c g c c c g c g c a c g g c t c a a c c g t g c g g c t g c a t g a a a t c c t g g c c g g t t t g t c t g a g c t c g g c g g g c g c g t g c c g a g t t g g c a c g c c g a c g t a c t t t a g g a c c g g c c a a a c a g a c

9180 a t g c c a a g c t g g c g g c c t g g c c g g c c a g c t t g g c c g c t g a a g a a a c c g a g c g c c g c c g t c t a c g g t t c g a c c g c c g g a c c g g c c g g t c g a a c c g g c g a c t t c t t t g g c t c g c g g c g g c a g

9240 t a a a a a g g t g a t g t g t a t t t g a g t a a a a c a g c t t g c g t c a t g c g g t c g c t g c g t a t a t g a a t t t t t c c a c t a c a c a t a a a c t c a t t t t g t c g a a c g c a g t a c g c c a g c g a c g c a t a t a c t

9300 1 5 MKVIAVLN pVS1 StaA MKVIAVLN t g c g a t g a g t a a a t a a a c a a a t a c g c a a g g g g a a c g c a t g a a g g t t a t c g c t g t a c t t a a a c g c t a c t c a t t t a t t t g t t t a t g c g t t c c c c t t g c g t a c t t c c a a t a g c g a c a t g a a t t

9360 10 15 20 25 QKGGSGKTTIATHLARALQL pVS1 StaA QKGGSGKTTIATHLARALQL c c a g a a a g g c g g g t c a g g c a a g a c g a c c a t c g c a a c c c a t c t a g c c c g c g c c c t g c a a c t g g t c t t t c c g c c c a g t c c g t t c t g c t g g t a g c g t t g g g t a g a t c g g g c g c g g g a c g t t g a

9420 30 35 40 45 AGADVLLVDSDPQGSARDWA pVS1 StaA AGADVLLVDSDPQGSARDWA c g c c g g g g c c g a t g t t c t g t t a g t c g a t t c c g a t c c c c a g g g c a g t g c c c g c g a t t g g g c g c g g c c c c g g c t a c a a g a c a a t c a g c t a a g g c t a g g g g t c c c g t c a c g g g c g c t a a c c c g

9480 50 55 60 65 AVREDQPLTVVGIDRPTIDR pVS1 StaA AVREDQPLTVVGIDRPTIDR g g c c g t g c g g g a a g a t c a a c c g c t a a c c g t t g t c g g c a t c g a c c g c c c g a c g a t t g a c c g c c g g c a c g c c c t t c t a g t t g g c g a t t g g c a a c a g c c g t a g c t g g c g g g c t g c t a a c t g g c

9540 70 75 80 85 DVKAIGRRDFVVIDGAPQAA pVS1 StaA DVKAIGRRDFVVIDGAPQAA c g a c g t g a a g g c c a t c g g c c g g c g c g a c t t c g t a g t g a t c g a c g g a g c g c c c c a g g c g g c g c t g c a c t t c c g g t a g c c g g c c g c g c t g a a g c a t c a c t a g c t g c c t c g c g g g g t c c g c c g

9600 90 95 100 105 DLAVSAIKAADFVLIPVQPS pVS1 StaA DLAVSAIKAADFVLIPVQPS g g a c t t g g c t g t g t c c g c g a t c a a g g c a g c c g a c t t c g t g c t g a t t c c g g t g c a g c c a a g c c t g a a c c g a c a c a g g c g c t a g t t c c g t c g g c t g a a g c a c g a c t a a g g c c a c g t c g g t t c

9660 110 115 120 125 PYDIWATADLVELVKQRIEV pVS1 StaA PYDIWATADLVELVKQRIEV c c c t t a c g a c a t a t g g g c c a c c g c c g a c c t g g t g g a g c t g g t t a a g c a g c g c a t t g a g g t g g g a a t g c t g t a t a c c c g g t g g c g g c t g g a c c a c c t c g a c c a a t t c g t c g c g t a a c t c c a

9720 130 135 140 145 TDGRLQAAFVVSRAIKGTRI pVS1 StaA TDGRLQAAFVVSRAIKGTRI c a c g g a t g g a a g g c t a c a a g c g g c c t t t g t c g t g t c g c g g g c g a t c a a a g g c a c g c g c a t g t g c c t a c c t t c c g a t g t t c g c c g g a a a c a g c a c a g c g c c c g c t a g t t t c c g t g c g c g t a

9780 150 155 160 165 GGEVAEALAGYELPILESRI pVS1 StaA GGEVAEALAGYELPILESRI c g g c g g t g a g g t t g c c g a g g c g c t g g c c g g g t a c g a g c t g c c c a t t c t t g a g t c c c g t a t g c c g c c a c t c c a a c g g c t c c g c g a c c g g c c c a t g c t c g a c g g g t a a g a a c t c a g g g c a t a

9840 170 175 180 185 TQRVSYPGTAAAGTTVLESE pVS1 StaA TQRVSYPGTAAAGTTVLESE c a c g c a g c g c g t g a g c t a c c c a g g c a c t g c c g c c g c c g g c a c a a c c g t t c t t g a a t c a g a g t g c g t c g c g c a c t c g a t g g g t c c g t g a c g g c g g c g g c c g t g t t g g c a a g a a c t t a g t c t

9900 190 195 200 205 PEGDAAREVQALAAEIKSKL pVS1 StaA PEGDAAREVQALAAEIKSKL a c c c g a g g g c g a c g c t g c c c g c g a g g t c c a g g c g c t g g c c g c t g a a a t t a a a t c a a a a c t t g g g c t c c c g c t g c g a c g g g c g c t c c a g g t c c g c g a c c g g c g a c t t t a a t t t a g t t t t g a

9960 210 I* pVS1 StaA I* c a t t t g a g t t a a t g a g g t a a a g a g a a a a t g a g c a a a a g c a c a a a c a c g c t a a g t g c c g g c g t a a a c t c a a t t a c t c c a t t t c t c t t t t a c t c g t t t t c g t g t t t g t g c g a t t c a c g g c c g

10,020 c g t c c g a g c g c a c g c a g c a g c a a g g c t g c a a c g t t g g c c a g c c t g g c a g a c a c g c c a g c c g c a g g c t c g c g t g c g t c g t c g t t c c g a c g t t g c a a c c g g t c g g a c c g t c t g t g c g g t c g g

10,080 a t g a a g c g g g t c a a c t t t c a g t t g c c g g c g g a g g a t c a c a c c a a g c t g a a g a t g t a c g c g t a c t t c g c c c a g t t g a a a g t c a a c g g c c g c c t c c t a g t g t g g t t c g a c t t c t a c a t g c g c

10,140 g t a c g c c a a g g c a a g a c c a t t a c c g a g c t g c t a t c t g a a t a c a t c g c g c a g c t a c c a g a g c a t g c g g t t c c g t t c t g g t a a t g g c t c g a c g a t a g a c t t a t g t a g c g c g t c g a t g g t c t c

10,200 t a a a t g a g c a a a t g a a t a a a t g a g t a g a t g a a t t t t a g c g g c t a a a g g a g g c g g c a t g g a a t t t a c t c g t t t a c t t a t t t a c t c a t c t a c t t a a a a t c g c c g a t t t c c t c c g c c g t a c c t

10,260 MPHVWRNGR *SCGPVSATSHGMHPPVPP a a a t c a a g a a c a a c c a g g c a c c g a c g c c g t g g a a t g c c c c a t g t g t g g a g g a a c g g g c g g t t t a g t t c t t g t t g g t c c g t g g c t g c g g c a c c t t a c g g g g t a c a c a c c t c c t t g c c c g c c

10,320 LARRKRLGCLPALQWHWNPQ QGPTLPQTAQRGQLPVPVGL t t g g c c a g g c g t a a g c g g c t g g g t t g c c t g c c g g c c c t g c a a t g g c a c t g g a a c c c c c a a a a c c g g t c c g c a t t c g c c g a c c c a a c g g a c g g c c g g g a c g t t a c c g t g a c c t t g g g g g t t

10,380 1 5 10 15 MSGRKPSGPVQIGAA pVS1 RepA ARGIGVSGRKPSGPVQIGAA GSSDAHATAFWGARYLDARR g c c c g a g g a a t c g g c g t g a g c g g t c g c a a a c c a t c c g g c c c g g t a c a a a t c g g c g c g g c g c g g g c t c c t t a g c c g c a c t c g c c a g c g t t t g g t a g g c c g g g c c a t g t t t a g c c g c g c c g c

10,440 20 25 30 35 LGDDLVEKLKAAQAAQRQRI pVS1 RepA LGDDLVEKLKAAQAAQRQRI QTIVQHLLQLGRLGGLPLAD c t g g g t g a t g a c c t g g t g g a g a a g t t g a a g g c c g c g c a g g c c g c c c a g c g g c a a c g c a t c g a c c c a c t a c t g g a c c a c c t c t t c a a c t t c c g g c g c g t c c g g c g g g t c g c c g t t g c g t a g

10,500 40 45 50 55 EAEARPGESWQAAADRIRKE pVS1 RepA EAEARPGESWQAAADRIRKE LCFCAGTFRPLRGSISDAFF g a g g c a g a a g c a c g c c c c g g t g a a t c g t g g c a a g c g g c c g c t g a t c g a a t c c g c a a a g a a c t c c g t c t t c g t g c g g g g c c a c t t a g c a c c g t t c g c c g g c g a c t a g c t t a g g c g t t t c t t

10,560 60 65 70 75 SRQPPAAGAPSIRKPPKGDE pVS1 RepA SRQPPAAGAPSIRKPPKGDE GPLRRCGTRRRNPLRGLAVL t c c c g g c a a c c g c c g g c a g c c g g t g c g c c g t c g a t t a g g a a g c c g c c c a a g g g c g a c g a g a g g g c c g t t g g c g g c c g t c g g c c a c g c g g c a g c t a a t c c t t c g g c g g g t t c c c g c t g c t c

10,620 80 85 90 95 QPDFFVPMLYDVGTRDSRSI pVS1 RepA QPDFFVPMLYDVGTRDSRSI LWIKENRHEIVHAGAITAAD c a a c c a g a t t t t t t c g t t c c g a t g c t c t a t g a c g t g g g c a c c c g c g a t a g t c g c a g c a t c g t t g g t c t a a a a a a g c a a g g c t a c g a g a t a c t g c a c c c g t g g g c g c t a t c a g c g t c g t a g

10,680 100 105 110 115 MDVAVFRLSKRDRRAGEVIR pVS1 RepA MDVAVFRLSKRDRRAGEVIR HVHGNETQRLTVSSSALHDA a t g g a c g t g g c c g t t t t c c g t c t g t c g a a g c g t g a c c g a c g a g c t g g c g a g g t g a t c c g c t a c c t g c a c c g g c a a a a g g c a g a c a g c t t c g c a c t g g c t g c t c g a c c g c t c c a c t a g g c g

10,740 120 125 130 135 YELPDGHVEVSAGPAGMASV pVS1 RepA YELPDGHVEVSAGPAGMASV VLKWVPVYLNGCPRGAHGTH t a c g a g c t t c c a g a c g g g c a c g t a g a g g t t t c c g c a g g g c c g g c c g g c a t g g c c a g t g t g a t g c t c g a a g g t c t g c c c g t g c a t c t c c a a a g g c g t c c c g g c c g g c c g t a c c g g t c a c a c

10,800 140 145 150 155 WDYDLVLMAVSHLTESMNRY pVS1 RepA WDYDLVLMAVSHLTESMNRY PIVVQYQHRNGM t g g g a t t a c g a c c t g g t a c t g a t g g c g g t t t c c c a t c t a a c c g a a t c c a t g a a c c g a t a c a c c c t a a t g c t g g a c c a t g a c t a c c g c c a a a g g g t a g a t t g g c t t a g g t a c t t g g c t a t g

10,860 160 165 170 175 REGKGDKPGRVFRPHVADVL pVS1 RepA REGKGDKPGRVFRPHVADVL c g g g a a g g g a a g g g a g a c a a g c c c g g c c g c g t g t t c c g t c c a c a c g t t g c g g a c g t a c t c g c c c t t c c c t t c c c t c t g t t c g g g c c g g c g c a c a a g g c a g g t g t g c a a c g c c t g c a t g a g

10,920 180 185 190 195 KFCRRADGGKQKDDLVETCI pVS1 RepA KFCRRADGGKQKDDLVETCI a a g t t c t g c c g g c g a g c c g a t g g c g g a a a g c a g a a a g a c g a c c t g g t a g a a a c c t g c a t t t t c a a g a c g g c c g c t c g g c t a c c g c c t t t c g t c t t t c t g c t g g a c c a t c t t t g g a c g t a a

BsiWI 10,980 200 205 210 215 RLNTTHVAMQRTKKAKNGRL pVS1 RepA RLNTTHVAMQRTKKAKNGRL c g g t t a a a c a c c a c g c a c g t t g c c a t g c a g c g t a c g a a g a a g g c c a a g a a c g g c c g c c t g g c c a a t t t g t g g t g c g t g c a a c g g t a c g t c g c a t g c t t c t t c c g g t t c t t g c c g g c g g a c

11,040 220 225 230 235 VTVSEGEALISRYKIVKSET pVS1 RepA VTVSEGEALISRYKIVKSET g t g a c g g t a t c c g a g g g t g a a g c c t t g a t t a g c c g c t a c a a g a t c g t a a a g a g c g a a a c c c a c t g c c a t a g g c t c c c a c t t c g g a a c t a a t c g g c g a t g t t c t a g c a t t t c t c g c t t t g g

BmtINheI 11,100 240 245 250 255 GRPEYIEIELADWMYREITE pVS1 RepA GRPEYIEIELADWMYREITE g g g c g g c c g g a g t a c a t c g a g a t c g a g c t a g c t g a t t g g a t g t a c c g c g a g a t c a c a g a a c c c g c c g g c c t c a t g t a g c t c t a g c t c g a t c g a c t a a c c t a c a t g g c g c t c t a g t g t c t t

BspDI*ClaI* 11,160 260 265 270 275 GKNPDVLTVHPDYFLIDPGI pVS1 RepA GKNPDVLTVHPDYFLIDPGI g g c a a g a a c c c g g a c g t g c t g a c g g t t c a c c c c g a t t a c t t t t t g a t c g a t c c c g g c a t c c c g t t c t t g g g c c t g c a c g a c t g c c a a g t g g g g c t a a t g a a a a a c t a g c t a g g g c c g t a g

11,220 280 285 290 295 GRFLYRLARRAAGKAEARWL pVS1 RepA GRFLYRLARRAAGKAEARWL g g c c g t t t t c t c t a c c g c c t g g c a c g c c g c g c c g c a g g c a a g g c a g a a g c c a g a t g g t t g c c g g c a a a a g a g a t g g c g g a c c g t g c g g c g c g g c g t c c g t t c c g t c t t c g g t c t a c c a a c

11,280 300 305 310 315 FKTIYERSGSAGEFKKFCFT pVS1 RepA FKTIYERSGSAGEFKKFCFT t t c a a g a c g a t c t a c g a a c g c a g t g g c a g c g c c g g a g a g t t c a a g a a g t t c t g t t t c a c c a a g t t c t g c t a g a t g c t t g c g t c a c c g t c g c g g c c t c t c a a g t t c t t c a a g a c a a a g t g g

FspI 11,340 320 325 330 335 VRKLIGSNDLPEYDLKEEAG pVS1 RepA VRKLIGSNDLPEYDLKEEAG g t g c g c a a g c t g a t c g g g t c a a a t g a c c t g c c g g a g t a c g a t t t g a a g g a g g a g g c g g g g c a c g c g t t c g a c t a g c c c a g t t t a c t g g a c g g c c t c a t g c t a a a c t t c c t c c t c c g c c c c

11,400 340 345 350 355 QAGPILVMRYRNLIEGEASA pVS1 RepA QAGPILVMRYRNLIEGEASA c a g g c t g g c c c g a t c c t a g t c a t g c g c t a c c g c a a c c t g a t c g a g g g c g a a g c a t c c g c c g t c c g a c c g g g c t a g g a t c a g t a c g c g a t g g c g t t g g a c t a g c t c c c g c t t c g t a g g c g g

11,460 GS* pVS1 RepA GS* g g t t c c t a a t g t a c g g a g c a g a t g c t a g g g c a a a t t g c c c t a g c a g g g g a a a a a g g t c g a c c a a g g a t t a c a t g c c t c g t c t a c g a t c c c g t t t a a c g g g a t c g t c c c c t t t t t c c a g c t

StuI 11,520 pVS1 oriV a a a g g c c t c t t t c c t g t g g a t a g c a c g t a c a t t g g g a a c c c a a a g c c g t a c a t t g g g a a c t t t c c g g a g a a a g g a c a c c t a t c g t g c a t g t a a c c c t t g g g t t t c g g c a t g t a a c c c t t g

AgeI 11,580 pVS1 oriV c g g a a c c c g t a c a t t g g g a a c c c a a a g c c g t a c a t t g g g a a c c g g t c a c a c a t g t a a g t g g c c t t g g g c a t g t a a c c c t t g g g t t t c g g c a t g t a a c c c t t g g c c a g t g t g t a c a t t c a c

11,640 pVS1 oriV a c t g a t a t a a a a g a g a a a a a a g g c g a t t t t t c c g c c t a a a a c t c t t t a a a a c t t a t t a a a t g a c t a t a t t t t c t c t t t t t t c c g c t a a a a a g g c g g a t t t t g a g a a a t t t t g a a t a a t t t

11,700 pVS1 oriV a c t c t t a a a a c c c g c c t g g c c t g t g c a t a a c t g t c t g g c c a g c g c a c a g c c c a a g a g c t g t g a g a a t t t t g g g c g g a c c g g a c a c g t a t t g a c a g a c c g g t c g c g t g t c g g g t t c t c g a c

11,760 c a a a a a g c g c c t a c c c t t c g g t c g c t g c g c t c c c t a c g c c c c g c c g c t t c g c g t c g g c c t g t t t t t c g c g g a t g g g a a g c c a g c g a c g c g a g g g a t g c g g g g c g g c g a a g c g c a g c c g g a

11,820 a t c g c g g c c g c t g g c c g c t c a a a a a t g g c t g g c c t a c g g c c a g g c a a t c t a c c a g g g c g c t a g c g c c g g c g a c c g g c g a g t t t t t a c c g a c c g g a t g c c g g t c c g t t a g a t g g t c c c g c g

KasINarISfoIPluTI 11,880 g g a c a a g c c g c g c c g t c g c c a c t c g a c c g c c g g c g c c c a c a t c a a g g c a c c c t g c c t c g c c c t g t t c g g c g c g g c a g c g g t g a g c t g g c g g c c g c g g g t g t a g t t c c g t g g g a c g g a g c g

11,940 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' g c g t t t c g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a a a c g g t c a c a g c c g c a a a g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t t t g c c a g t g t c g

12,000 t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c

12,060 bom c g g g t g t c g g g g c g c a g c c a t g a c c c a g t c a c g t a g c g a t a g c g g a g t g t a t a c t g g c t t g c c c a c a g c c c c g c g t c g g t a c t g g g t c a g t g c a t c g c t a t c g c c t c a c a t a t g a c c g a a

12,120 bom C G G C A T C A G A G C A G A T T G T A pRS-marker a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g c g g t g t g a a a t a c c g t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c g c c a c a c t t t a t g g c

12,180 bom G A G C G A G T G A C T G L4440 c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a g g c c c t c t t c c g c t t c c t c g c t c a c t g a c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t c c g g g a g a a g g c g a a g g a g c g a g t g a c t g

12,240 A G C G A L4440 t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t

12,300 c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t

12,360 ori a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a

12,420 ori T T pBR322ori-F g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t

12,480 ori T C T A T G G T C C G C A A A G G G pBR322ori-F a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c

12,540 ori c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t

12,600 ori c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t

3ʹ 5ʹ 12,635 ori c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.