Addgene Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD


Map View

800 600 400 200 End (936) BspEI (899) AcuI (830) BfuAI - BspMI (824) PstI (821) AhdI (819) BbsI (818) SfcI (817) BsmI (785) BsaI (766) PfoI * (736) BmrI (686) PvuII (663) AlwNI (625) PpuMI (596) BsgI (561) SacII (539) XcmI (538) EarI - SapI - BspQI (526) PspFI (500) BseYI (496) BsrBI (488) XmnI (475) SmaI (419) XmaI - TspMI (417) BaeGI Bme1580I (357) BstXI (312) BsiHKAI (254) MmeI (251) NmeAIII (189) MscI (161) EaeI (159) MflI * - BstYI - BamHI (150) BsaBI * (149) NspI (124) PflFI - Tth111I (91) ClaI - BspDI (68) PsiI (53) KpnI (20) BanI - Acc65I (16) HindIII (10) AflII (7) Start (0) 3xFLAG Tet-inducible 3xFlag Mouse C325A Caspase-9 Δ CARD 936 bp

Sequence View

PsiIStart (0) AflIIHindIIIAcc65IBanIKpnI 60 1 5 10 DYKDHDGDYKD 3xFLAG FKLKLGTAMDYKDHDGDYKD KFKLKTGGHVVFVMVTIIFI t t t a a a c t t a a g c t t g g t a c c g c c a t g g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t a a a t t t g a a t t c g a a c c a t g g c g g t a c c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a

Tth111IPflFIBspDIClaI 120 15 20 HDIDYKDDDDK (in frame with 3xFLAG) VCVPGKIRG 3xFLAG HDIDYKDDDDKVCVPGKIRG MVDIVLIVIVLDTNWPLDPS c a t g a c a t c g a t t a c a a g g a t g a c g a t g a c a a g g t c t g t g t t c c a g g g a a g a t c a g g g g a g t a c t g t a g c t a a t g t t c c t a c t g c t a c t g t t c c a g a c a c a a g g t c c c t t c t a g t c c c c t

EaeIMscIBsaBI*BamHIBstYIMflI*NspI 180 (in frame with 3xFLAG) HADMAYTLDSDPCGHCLIIN HADMAYTLDSDPCGHCLIIN MCIHCVGQIRIGTAVAEDDV c a t g c a g a t a t g g c a t a c a c c c t g g a t t c g g a t c c c t g t g g c c a c t g c c t c a t c a t c a a c g t a c g t c t a t a c c g t a t g t g g g a c c t a a g c c t a g g g a c a c c g g t g a c g g a g t a g t a g t t g

NmeAIII 240 (in frame with 3xFLAG) NVNFCPSSGLGTRTGSNLDR NVNFCPSSGLGTRTGSNLDR IHVEARGRPEACARAGVQVT a a t g t g a a c t t c t g c c c t t c c t c g g g g c t c g g c a c a c g c a c g g g c t c c a a c t t g g a c c g t t t a c a c t t g a a g a c g g g a a g g a g c c c c g a g c c g t g t g c g t g c c c g a g g t t g a a c c t g g c a

MmeIBsiHKAI 300 (in frame with 3xFLAG) DKLEHRFRWLRFMVEVKNDL DKLEHRFRWLRFMVEVKNDL VFKLVSEAPQAEHHLHLVVQ g a c a a a c t t g a g c a c c g a t t c c g c t g g c t g c g c t t c a t g g t g g a g g t g a a g a a c g a c c t g c t g t t t g a a c t c g t g g c t a a g g c g a c c g a c g c g a a g t a c c a c c t c c a c t t c t t g c t g g a c

Bme1580IBaeGIBstXI 360 (in frame with 3xFLAG) TAKKMVTALMEMAHRNHRAL TAKKMVTALMEMAHRNHRAL SGLFHDRSQHLHCVPVVTGQ a c t g c c a a g a a a a t g g t c a c g g c t t t g a t g g a g a t g g c a c a c c g g a a c c a c c g t g c c c t g t g a c g g t t c t t t t a c c a g t g c c g a a a c t a c c t c t a c c g t g t g g c c t t g g t g g c a c g g g a c

TspMIXmaISmaI 420 (in frame with 3xFLAG) DCFVVVILSHGCQASHLQFP DCFVVVILSHGCQASHLQFP VAKHHDDERMAALGAVELER g a c t g c t t t g t g g t g g t c a t c c t c t c t c a t g g c t g c c a g g c c a g c c a c c t c c a g t t c c c g c t g a c g a a a c a c c a c c a g t a g g a g a g a g t a c c g a c g g t c c g g t c g g t g g a g g t c a a g g g c

XmnI 480 (in frame with 3xFLAG) GAVYGTDGCSVSIEKIVNIF GAVYGTDGCSVSIEKIVNIF TSDIPCISAGHGNLFNHIDE g g t g c t g t c t a t g g g a c a g a t g g a t g c t c c g t g t c c a t t g a g a a a a t t g t g a a t a t c t t c c c a c g a c a g a t a c c c t g t c t a c c t a c g a g g c a c a g g t a a c t c t t t t a a c a c t t a t a g a a g

XcmISacIIBspQISapIEarIBseYIPspFIBsrBI 540 (in frame with 3xFLAG) NGSGCPSLGGKPKLFFIQAA NGSGCPSLGGKPKLFFIQAA VPAAAGAQSPLGLEEEDLGR a a c g g g a g c g g c t g c c c c a g c c t g g g a g g g a a g c c c a a g c t c t t c t t c a t c c a g g c c g c g t t g c c c t c g c c g a c g g g g t c g g a c c c t c c c t t c g g g t t c g a g a a g a a g t a g g t c c g g c g c

PpuMIBsgI 600 (in frame with 3xFLAG) GGEQKDHGFEVACTSSQGRT GGEQKDHGFEVACTSSQGRT TTLLFVM g g t g g t g a g c a g a a a g a c c a t g g c t t t g a g g t g g c c t g c a c t t c c t c t c a a g g c a g g a c c c c a c c a c t c g t c t t t c t g g t a c c g a a a c t c c a c c g g a c g t g a a g g a g a g t t c c g t c c t g g

AlwNI 660 (in frame with 3xFLAG) LDSDSEPDAVPYQEGPRPLD LDSDSEPDAVPYQEGPRPLD t t g g a c a g t g a c t c t g a g c c a g a t g c t g t c c c c t a t c a g g a a g g c c c a a g g c c c t t g g a c a a c c t g t c a c t g a g a c t c g g t c t a c g a c a g g g g a t a g t c c t t c c g g g t t c c g g g a a c c t g

BmrIPvuII 720 (in frame with 3xFLAG) QLDAVSSLPTPSDILVSYST QLDAVSSLPTPSDILVSYST c a g c t g g a t g c t g t g t c a a g t t t g c c t a c c c c c a g t g a c a t c c t t g t g t c c t a c t c c a c c g t c g a c c t a c g a c a c a g t t c a a a c g g a t g g g g g t c a c t g t a g g a a c a c a g g a t g a g g t g g

BsaIPfoI* 780 (in frame with 3xFLAG) FPGFVSWRDKKSGSWYIETL FPGFVSWRDKKSGSWYIETL t t c c c a g g t t t t g t c t c c t g g a g g g a c a a g a a a a g t g g c t c c t g g t a c a t c g a g a c c t t g a a g g g t c c a a a a c a g a g g a c c t c c c t g t t c t t t t c a c c g a g g a c c a t g t a g c t c t g g a a c

AcuISfcIBbsIAhdIPstIBspMIBfuAIBsmI 840 (in frame with 3xFLAG) DGILEQWARSEDLQSLLLRV DGILEQWARSEDLQSLLLRV g a t g g c a t t c t g g a g c a g t g g g c t c g c t c t g a a g a c c t g c a g t c c c t c c t t c t c a g g g t t c t a c c g t a a g a c c t c g t c a c c c g a g c g a g a c t t c t g g a c g t c a g g g a g g a a g a g t c c c a a

BspEI 900 (in frame with 3xFLAG) ANAVSAKGTYKQIPGCFNFL ANAVSAKGTYKQIPGCFNFL g c c a a t g c t g t t t c t g c g a a a g g g a c t t a c a a g c a g a t t c c t g g c t g t t t t a a c t t c c t c c g g t t a c g a c a a a g a c g c t t t c c c t g a a t g t t c g t c t a a g g a c c g a c a a a a t t g a a g g a g

End (936) 936 (in frame with 3xFLAG) RKKLFFKTS* RKKLFFKTS* c g g a a a a a g c t g t t t t t t a a a a c t t c a t g a g g c t c g g c c t t t t t c g a c a a a a a a t t t t g a a g t a c t c c g a g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.