Addgene pAGT5439 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAGT5439 Sequencing Result


Map View

AhdI (2770) AflIII - PciI (2769) ApaI - BanII (2695) EcoO109I (2692) PspOMI (2691) EagI (2634) BanI (2607) MreI - SgrAI (2542) PasI (2471) AgeI (2415) MfeI (2368) BsrGI - TatI (2287) Bpu10I (2158) DrdI (1971) pBR322ori-F (1918 .. 1937) BssSI - BssSαI (1900) BciVI (1875) PspFI (1773) BseYI (1769) AlwNI (1664) PaeR7I - XhoI (2793) NmeAIII (40) TaqII (123) BclI * (493) BpmI (547) PvuI (676) BtgZI (853) BsaAI (1052) BspHI (1353) pAGT5439 2794 bp

Sequence View

NmeAIII 5ʹ 3ʹ 60 c g a g a c c a c g a a g t g c c g a g g c a c g a a c c c a g t g g a c a t a a g c c t g t t c g g t t c g t a a g c g c t c t g g t g c t t c a c g g c t c c g t g c t t g g g t c a c c t g t a t t c g g a c a a g c c a a g c a t t c g

120 MRSRNWSRTLTERS t g t a a t g c a a g t a g c g t a t g c g c t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g a c a t t a c g t t c a t c g c a t a c g c g a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c

TaqII 180 GGNGAVAVFMACYDCFFGVQ g t g g t a a c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g g t a c a g t c a c c a t t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c c a t g t c a

240 SMPRASKQQARYAVGRCLML c t a t g c c t c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g a t a c g g a g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a

300 1 M SmR WSSNDVTQQGSRPKTKLNIM g g a g c a g c a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t c a t g g c c t c g t c g t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a g t a c c

360 5 10 15 20 GEAVIAEVSTQLSEVVGVIE SmR GEAVIAEVSTQLSEVVGVIE g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t a g t t g g c g t c a t c g a g c c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a t c a a c c g c a g t a g c t c g

420 25 30 35 40 RHLEPTLLAVHLYGSAVDGG SmR RHLEPTLLAVHLYGSAVDGG g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c c g c a g t g g a t g g c g g c c c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g g c g t c a c c t a c c g c c g g

480 45 50 55 60 LKPHSDIDLLVTVTVRLDET SmR LKPHSDIDLLVTVTVRLDET t g a a g c c a c a c a g c g a t a t t g a t t t g c t g g t t a c g g t g a c c g t a a g g c t t g a t g a a a c a a a c t t c g g t g t g t c g c t a t a a c t a a a c g a c c a a t g c c a c t g g c a t t c c g a a c t a c t t t g t t

BclI* 540 65 70 75 80 TRRALINDLLETSASPGESE SmR TRRALINDLLETSASPGESE c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c c c c t g g a g a g a g c g a g a g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g g g g a c c t c t c t c g c t c t

BpmI 600 85 90 95 100 ILRAVEVTIVVHDDIIPWRY SmR ILRAVEVTIVVHDDIIPWRY t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t c a t t c c g t g g c g t t a t c a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a g t a a g g c a c c g c a a t a g

660 105 110 115 120 PAKRELQFGEWQRNDILAGI SmR PAKRELQFGEWQRNDILAGI c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a c a t t c t t g c a g g t a t c t g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t g t a a g a a c g t c c a t a g a

PvuI 720 125 130 135 140 FEPATIDIDLAILLTKAREH SmR FEPATIDIDLAILLTKAREH t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c a a a a g c a a g a g a a c a t a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g t t t t c g t t c t c t t g t a t

780 145 150 155 160 SVALVGPAAEELFDPVPEQD SmR SVALVGPAAEELFDPVPEQD g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c g g t t c c t g a a c a g g a t c c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g c c a a g g a c t t g t c c t a g

840 165 170 175 180 LFEALNETLTLWNSPPDWAG SmR LFEALNETLTLWNSPPDWAG t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c g c c c g a c t g g g c t g g c g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g c g g g c t g a c c c g a c c g c

BtgZI 900 185 190 195 200 DERNVVLTLSRIWYSAVTGK SmR DERNVVLTLSRIWYSAVTGK a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g c a g t a a c c g g c a a a a t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c g c g t c a t t g g c c g t t t t

960 205 210 215 220 IAPKDVAADWAMERLPAQYQ SmR IAPKDVAADWAMERLPAQYQ t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c c g g c c c a g t a t c a g c a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a c g g c c g g g t c a t a g t c g

1020 225 230 235 240 PVILEARQAYLGQEEDRLAS SmR PVILEARQAYLGQEEDRLAS c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a a g a t c g c t t g g c c t c g c g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t t c t a g c g a a c c g g a g c g

BsaAI 1080 245 250 255 260 RADQLEEFVHYVKGEITKVV SmR RADQLEEFVHYVKGEITKVV g c g c a g a t c a g t t g g a a g a a t t t g t c c a t t a c g t g a a a g g c g a g a t c a c c a a g g t a g t c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t a a t g c a c t t t c c g c t c t a g t g g t t c c a t c a g c

1140 GK* SmR GK* g c a a a t a a t g t c t a g c t a g a a a t t c g t t c a a g c c g a c g c c g c t t c g c g g c g c g g c t t a a c c g t t t a t t a c a g a t c g a t c t t t a a g c a a g t t c g g c t g c g g c g a a g c g c c g c g c c g a a t t g

1200 t c a a g c g t t a g a t g c a c t a a g c a c a t a a t t g c t c a c a g c c a a a c t a t c a g g t c a a g t c t g a g t t c g c a a t c t a c g t g a t t c g t g t a t t a a c g a g t g t c g g t t t g a t a g t c c a g t t c a g a c

1260 c t t t t a t t a t t t t t a a g c g t g c a t a a t a a g c c c t a c a c a a a t t g g g a g a t a t a t c a t g c t g a a a a t a a t a a a a a t t c g c a c g t a t t a t t c g g g a t g t g t t t a a c c c t c t a t a t a g t a c g a

1320 g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t

BspHI 1380 a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a

1440 ori t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a

1500 ori t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c

1560 ori t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t

1620 ori g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a

AlwNI 1680 ori a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t

1740 ori t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g

BseYIPspFI 1800 ori g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a

1860 ori g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t

BssSαIBssSIBciVI 1920 ori G G G pBR322ori-F c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c

DrdI 1980 ori A A A C G C C T G G T A T C T T T pBR322ori-F a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a

2040 ori t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a

2100 OCS terminator a c g g t t c c t g c a c t c t g t g g t c t c a t t c g t g a g c t t g t c c t g c t t t a a t g a g a t a t g c g a t g c c a a g g a c g t g a g a c a c c a g a g t a a g c a c t c g a a c a g g a c g a a a t t a c t c t a t a c g c t

Bpu10I 2160 OCS terminator g a a g c c t a t g a t c g c a t g a t a t t t g c t t t c a a t t c t g t t g t g c a c g t t g t a a a a a a c c t g c t t c g g a t a c t a g c g t a c t a t a a a c g a a a g t t a a g a c a a c a c g t g c a a c a t t t t t t g g a c

2220 OCS terminator a g c a t g t g t a g c t c a g a t c c t t a c c g c c g g t t t c g g t t c a t t c t a a t g a a t a t a t c a c c c t c g t a c a c a t c g a g t c t a g g a a t g g c g g c c a a a g c c a a g t a a g a t t a c t t a t a t a g t g g g

2280 OCS terminator g t t a c t a t c g t a t t t t t a t g a a t a a t a t t c t c c g t t c a a t t t a c t g a t t g t a c c c t a c t a c a a t g a t a g c a t a a a a a t a c t t a t t a t a a g a g g c a a g t t a a a t g a c t a a c a t g g g a t g a t

BsrGITatI 2340 OCS terminator c t t a t a t g t a c a a t a t t a a a a t g a a a a c a a t a t a t t g t g c t g a a t a g g t t t a t a g c g a c a g a a t a t a c a t g t t a t a a t t t t a c t t t t g t t a t a t a a c a c g a c t t a t c c a a a t a t c g c t g t

MfeI 2400 OCS terminator t c t a t g a t a g a g c g c c a c a a t a a c a a a c a a t t g c g t t t t a t t a t t a c a a a t c c a a t t t t a a g a t a c t a t c t c g c g g t g t t a t t g t t t g t t a a c g c a a a a t a a t a a t g t t t a g g t t a a a a t

AgeI 2460 OCS terminator a a a a a a g c g g c a g a a c c g g t c a a a c c t a a a a g a c t g a t t a c a t a a a t c t t a t t c a a a t t t t t t t t t c g c c g t c t t g g c c a g t t t g g a t t t t c t g a c t a a t g t a t t t a g a a t a a g t t t a a a

PasI 2520 OCS terminator c a a a a g t g c c c c a g g g g c t a g t a t c t a c g a c a c a c c g a g c g g c g a a c t a a t a a c g c t c a c g t t t t c a c g g g g t c c c c g a t c a t a g a t g c t g t g t g g c t c g c c g c t t g a t t a t t g c g a g t g

SgrAIMreI 2580 OCS terminator t g a a g g g a a c t c c g g t t c c c c g c c g g c g c g c a t g g g t g a g a t t c c t t g a a g t t g a g t a t t a c t t c c c t t g a g g c c a a g g g g c g g c c g c g c g t a c c c a c t c t a a g g a a c t t c a a c t c a t a a

EagIBanI 2640 OCS terminator g g c c g t c c g c t c t a c c g a a a g t t a c g g g c a c c a t t c a a c c c g g t c c a g c a c g g c g g c c g g c c g g c a g g c g a g a t g g c t t t c a a t g c c c g t g g t a a g t t g g g c c a g g t c g t g c c g c c g g c c

PspOMIEcoO109IBanIIApaI 2700 OCS terminator g t a a c c g a c t t g c t g c c c c g a g a a t t a t g c a g c a t t t t t t t g g t g t a t g t g g g c c c c a a a c a t t g g c t g a a c g a c g g g g c t c t t a a t a c g t c g t a a a a a a a c c a c a t a c a c c c g g g g t t t

2760 OCS terminator t g a a g t g c a g g t c a a a c c t t g a c a g t g a c g a c a a a t c g t t g g g c g g g t c c a g g g c g a a t t a c t t c a c g t c c a g t t t g g a a c t g t c a c t g c t g t t t a g c a a c c c g c c c a g g t c c c g c t t a a

PaeR7IXhoIAflIIIPciIAhdI 3ʹ 5ʹ 2794 OCS terminator t t g c g a c a a c a t g t c g a g g c t c a g c a g g a c c g c t a a c g c t g t t g t a c a g c t c c g a g t c g t c c t g g c g a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.