Addgene CPSF5 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: CPSF5 Sequencing Result


Map View

SgrDI (6818) SspI (6701) Amp-R (6447 .. 6466) AhdI (5897) pBR322ori-F (5145 .. 5164) L4440 (4894 .. 4911) CAP binding site M13/pUC Reverse (4676 .. 4698) lac operator M13 Reverse (4663 .. 4679) BstZ17I (4625) EBV-rev (4584 .. 4603) BsmI (4573) SV40pA-R (4530 .. 4549) PsiI (4512) PfoI (4428) NdeI (3788) RsrII (3746) AsiSI (3702) NcoI (3690) pRS-marker (6864 .. 6883) AflII (499) FseI (824) PasI (868) KasI (1058) NarI (1059) SfoI (1060) PluTI (1062) EF1a-F (1246 .. 1266) T7 (1315 .. 1334) BamHI (1372) SpeI (1378) TspMI - XmaI (1482) SmaI (1484) CsiI - SexAI * (1759) Bsu36I (1944) PflMI * (2063) NotI (2170) PspXI (2176) BstBI (2199) AfeI (2269) BmgBI (2380) BsaBI * (2575) SfiI (2696) BsiWI (2735) PmeI (2756) BclI * (2765) BGH-rev (2772 .. 2789) BbsI (2967) SphI (2978) F1ori-R (3135 .. 3154) MscI (3338) PshAI (3365) BsmBI - Esp3I (3406) CPSF5 7051 bp

Sequence View

60 g t t g a c a t t g a t t a t t g a c t a g g c t t t t g c a a a a a g c t t t g c a a a g a t g g a t a a a g t t t t c a a c t g t a a c t a a t a a c t g a t c c g a a a a c g t t t t t c g a a a c g t t t c t a c c t a t t t c a a a a

120 EF-1 α promoter a a a c a g a g a g g a a t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g t t t g t c t c t c c t t a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c

180 EF-1 α promoter g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c

240 EF-1 α promoter g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c

300 EF-1 α promoter a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c

360 EF-1 α promoter EF-1 α intron A t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c

420 EF-1 α promoter EF-1 α intron A * t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t

480 EF-1 α promoter EF-1 α intron A KWRAATRSEQDRAEPQFHTP c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c

AflII 540 EF-1 α promoter EF-1 α intron A SNSAKRKLLGKAEHKLQPRA a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g

600 EF-1 α promoter EF-1 α intron A QASPGGRAFRTAGERRDRQK c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a

660 EF-1 α promoter EF-1 α intron A RYTELWKFNKIVQQSAKKRA t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g

720 EF-1 α promoter EF-1 α intron A LYDQLHPGLDACQYKPKQPR a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c

780 EF-1 α promoter EF-1 α intron A PRRRPGHTGACMNPSAPGAL c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c

FseI 840 EF-1 α promoter EF-1 α intron A AAVSFRVPTTELQGAQEPAQ c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c

PasI 900 EF-1 α promoter EF-1 α intron A GRAATYRGARPPLAPGTPVL g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c

960 EF-1 α promoter EF-1 α intron A QTLPFIAAERGQQLSSLISS t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t

1020 EF-1 α promoter EF-1 α intron A AASPLAPPHTVWVFSFPRET c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a

KasINarISfoIPluTI 1080 EF-1 α promoter EF-1 α intron A RLRRKM c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a

1140 EF-1 α promoter EF-1 α intron A a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c

1200 EF-1 α promoter EF-1 α intron A a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a

1260 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G EF1a-F t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c

1320 EF-1 α promoter T7 promoter EF-1 α intron A T G G T T C EF1a-F T A A T A C T7 t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t a g c t t g g t a c t a a t a c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a t c g a a c c a t g a t t a t g

SpeIBamHI 1380 T7 promoter G A C T C A C T A T A G G G T7 g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g g t a a g c t t g g t a c c g a g c t c g g a t c c a c t c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c c a t t c g a a c c a t g g c t c g a g c c t a g g t g a

1440 attB1 a g t c c a g t g t g g t g g a a t t c t g c a g a t a t c a a c a a g t t t g t a c a a a a a a g c a g g c t c c a c t c a g g t c a c a c c a c c t t a a g a c g t c t a t a g t t g t t c a a a c a t g t t t t t t c g t c c g a g g t g

TspMIXmaISmaI 1500 MSVVPPNRSQTGWPRGVTQF c a t g t c t g t g g t a c c g c c c a a t c g c t c g c a g a c c g g c t g g c c c c g g g g g g t c a c t c a g t t g t a c a g a c a c c a t g g c g g g t t a g c g a g c g t c t g g c c g a c c g g g g c c c c c c a g t g a g t c a a

1560 GNKYIQQTKPLTLERTINLY c g g c a a c a a g t a c a t c c a g c a g a c g a a g c c c c t c a c c c t g g a g c g c a c c a t c a a c c t g t a g c c g t t g t t c a t g t a g g t c g t c t g c t t c g g g g a g t g g g a c c t c g c g t g g t a g t t g g a c a t

1620 PLTNYTFGTKEPLYEKDSSV c c c t c t t a c c a a t t a t a c t t t t g g t a c a a a a g a g c c c c t c t a c g a g a a g g a c a g c t c t g t g g g a g a a t g g t t a a t a t g a a a a c c a t g t t t t c t c g g g g a g a t g c t c t t c c t g t c g a g a c a

1680 AARFQRMREEFDKIGMRRTV t g c a g c c a g a t t t c a g c g c a t g a g g g a a g a a t t t g a t a a a a t t g g a a t g a g g a g g a c t g t a c g t c g g t c t a a a g t c g c g t a c t c c c t t c t t a a a c t a t t t t a a c c t t a c t c c t c c t g a c a

1740 EGVLIVHEHRLPHVLLLQLG a g a a g g g g t t c t g a t t g t a c a t g a g c a c c g g c t a c c c c a t g t g t t a c t g c t g c a g c t g g g t c t t c c c c a a g a c t a a c a t g t a c t c g t g g c c g a t g g g g t a c a c a a t g a c g a c g t c g a c c c

SexAI*CsiI 1800 TTFFKLPGGELNPGEDEVEG a a c a a c t t t c t t c a a a c t a c c t g g t g g t g a a c t t a a c c c a g g a g a a g a t g a a g t t g a a g g t t g t t g a a a g a a g t t t g a t g g a c c a c c a c t t g a a t t g g g t c c t c t t c t a c t t c a a c t t c c

1860 LKRLMTEILGRQDGVLQDWV a c t a a a a c g c t t a a t g a c a g a g a t a c t g g g t c g t c a g g a t g g a g t t t t g c a a g a c t g g g t t g a t t t t g c g a a t t a c t g t c t c t a t g a c c c a g c a g t c c t a c c t c a a a a c g t t c t g a c c c a

1920 IDDCIGNWWRPNFEPPQYPY c a t t g a c g a t t g c a t t g g t a a c t g g t g g a g a c c a a a t t t t g a a c c t c c t c a g t a t c c a t a g t a a c t g c t a a c g t a a c c a t t g a c c a c c t c t g g t t t a a a a c t t g g a g g a g t c a t a g g t a t

Bsu36I 1980 IPAHITKPKEHKKLFLVQLQ t a t t c c t g c a c a t a t t a c a a a g c c t a a g g a a c a t a a g a a g t t g t t t c t g g t t c a g c t t c a a t a a g g a c g t g t a t a a t g t t t c g g a t t c c t t g t a t t c t t c a a c a a a g a c c a a g t c g a a g t

2040 EKALFAVPKNYKLVAAPLFE a g a a a a a g c c t t g t t t g c a g t c c c t a a a a a t t a c a a g c t g g t a g c t g c a c c a t t g t t t g a t c t t t t t c g g a a c a a a c g t c a g g g a t t t t t a a t g t t c g a c c a t c g a c g t g g t a a c a a a c t

PflMI* 2100 LYDNAPGYGPIISSLPQLLS a t t g t a t g a c a a t g c a c c a g g a t a t g g a c c c a t c a t t t c t a g t c t c c c t c a g c t g t t g a g t a a c a t a c t g t t a c g t g g t c c t a t a c c t g g g t a g t a a a g a t c a g a g g g a g t c g a c a a c t c

2160 attB2 RFNFIYNLDPAFLYKVVDIQ c a g g t t c a a t t t t a t t t a c a a c t t g g a c c c a g c t t t c t t g t a c a a a g t g g t t g a t a t c c a g t c c a a g t t a a a a t a a a t g t t g a a c c t g g g t c g a a a g a a c a t g t t t c a c c a a c t a t a g g t

BstBIPspXINotI 2220 HSGGRSSLEGPRFETSGGGG g c a c a g t g g c g g c c g c t c g a g t c t a g a g g g c c c g c g g t t c g a a a c c a g c g g a g g c g g c g g c g t g t c a c c g c c g g c g a g c t c a g a t c t c c c g g g c g c c a a g c t t t g g t c g c c t c c g c c g c c

AfeI 2280 1 GK V5 tag SGGGGSGGGGSGGGGSATGK a a g c g g g g g a g g a g g c a g c g g c g g a g g c g g c a g c g g g g g c g g c g g a a g c g c t a c c g g t a a t t c g c c c c c t c c t c c g t c g c c g c c t c c g c c g t c g c c c c c g c c g c c t t c g c g a t g g c c a t t

2340 5 10 PIPNPLLGLDST (in frame with V5 tag) RTGMASNF V5 tag PIPNPLLGLDSTRTGMASNF g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g c g t a c c g g t a t g g c t t c t a a c t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c g c a t g g c c a t a c c g a a g a t t g a a

BmgBI 2400 (in frame with V5 tag) TQFVLVDNGGTGDVTVAPSN TQFVLVDNGGTGDVTVAPSN t a c t c a g t t c g t t c t c g t c g a c a a t g g c g g a a c t g g c g a c g t g a c t g t c g c c c c a a g c a a a t g a g t c a a g c a a g a g c a g c t g t t a c c g c c t t g a c c g c t g c a c t g a c a g c g g g g t t c g t t

2460 (in frame with V5 tag) FANGVAEWISSNSRSQAYKV FANGVAEWISSNSRSQAYKV c t t c g c t a a c g g g g t c g c t g a a t g g a t c a g c t c t a a c t c g c g a t c a c a g g c t t a c a a a g t g a a g c g a t t g c c c c a g c g a c t t a c c t a g t c g a g a t t g a g c g c t a g t g t c c g a a t g t t t c a

2520 (in frame with V5 tag) TCSVRQSSAQNRKYTIKVEV TCSVRQSSAQNRKYTIKVEV a a c c t g t a g c g t t c g t c a g a g c t c t g c g c a g a a t c g c a a a t a c a c c a t c a a a g t c g a g g t t t g g a c a t c g c a a g c a g t c t c g a g a c g c g t c t t a g c g t t t a t g t g g t a g t t t c a g c t c c a

BsaBI* 2580 (in frame with V5 tag) PKVATQTVGGEELPVAGWRS PKVATQTVGGEELPVAGWRS g c c t a a a g t g g c a a c c c a g a c t g t t g g t g g t g a a g a g c t t c c t g t a g c c g g a t g g a g a t c c g g a t t t c a c c g t t g g g t c t g a c a a c c a c c a c t t c t c g a a g g a c a t c g g c c t a c c t c t a g

2640 (in frame with V5 tag) YLNMELTIPIFATNSDCELI YLNMELTIPIFATNSDCELI t t a c t t a a a t a t g g a a c t a a c c a t t c c a a t t t t c g c c a c g a a t t c c g a c t g c g a g c t t a t a a t g a a t t t a t a c c t t g a t t g g t a a g g t t a a a a g c g g t g c t t a a g g c t g a c g c t c g a a t a

SfiI 2700 (in frame with V5 tag) VKAMQGLLKDGNPIPSAIAA VKAMQGLLKDGNPIPSAIAA t g t t a a g g c a a t g c a a g g t c t c c t a a a a g a t g g a a a c c c g a t t c c c t c g g c c a t c g c g g c a c a a t t c c g t t a c g t t c c a g a g g a t t t t c t a c c t t t g g g c t a a g g g a g c c g g t a g c g c c g

PmeIBsiWI 2760 (in frame with V5 tag) NSGIYGGGGGSRTG* NSGIYGGGGGSRTG* c a a c t c c g g c a t c t a c g g a g g t g g a g g t g g a t c t c g t a c g g g t t a g t a a t g a g t t t a a a c g t t g a g g c c g t a g a t g c c t c c a c c t c c a c c t a g a g c a t g c c c a a t c a t t a c t c a a a t t t g

BclI* 2820 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g

2880 bGH poly(A) signal c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t

2940 bGH poly(A) signal a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t

SphIBbsI 3000 bGH poly(A) signal c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a

3060 bGH poly(A) signal g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c

3120 c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c

3180 G A G G A A A G C G A A A G A A G G G A F1ori-R c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a

3240 t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t

3300 FRT c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a c c t a g a a g t t c c t a t t c c g g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t g g a t c t t c a a g g a t a a g g c

MscI 3360 5 KKPELTA FRT HygR 5 KKPELTA HygR *FTYSSGQGFLRFEGR a a g t t c c t a t t c t c t a g a a a g t a t a g g a a c t t c c t t g g c c a a a a a g c c t g a a c t c a c c g c t t c a a g g a t a a g a g a t c t t t c a t a t c c t t g a a g g a a c c g g t t t t t c g g a c t t g a g t g g c g

BsmBIEsp3IPshAI 3420 10 15 20 25 TSVEKFLIEKFDSVSDLMQL HygR 10 15 20 25 TSVEKFLIEKFDSVSDLMQL HygR MQL RRDLLKQDFLEVADGVQHLE g a c g t c t g t c g a g a a g t t t c t g a t c g a a a a g t t c g a c a g c g t c t c c g a c c t g a t g c a g c t c t g c a g a c a g c t c t t c a a a g a c t a g c t t t t c a a g c t g t c g c a g a g g c t g g a c t a c g t c g a

3480 30 35 40 45 SEGEESRAFSFDVGGRGYVL HygR 30 35 40 45 SEGEESRAFSFDVGGRGYVL HygR SEGEESRAFSFDVGGRGYVL RLAFFRTSEAEIYSPTSIDQ c t c g g a g g g c g a a g a a t c t c g t g c t t t c a g c t t c g a t g t a g g a g g g c g t g g a t a t g t c c t g a g c c t c c c g c t t c t t a g a g c a c g a a a g t c g a a g c t a c a t c c t c c c g c a c c t a t a c a g g a

3540 50 55 60 65 RVNSCADGFYKDRYVYRHFA HygR 50 55 60 65 RVNSCADGFYKDRYVYRHFA HygR RVNSCADGFYKDRYVYRHFA PYIAAGITEVFITINIPVKC g c g g g t a a a t a g c t g c g c c g a t g g t t t c t a c a a a g a t c g t t a t g t t t a t c g g c a c t t t g c c g c c c a t t t a t c g a c g c g g c t a c c a a a g a t g t t t c t a g c a a t a c a a a t a g c c g t g a a a c g

3600 70 75 80 85 SAALPIPEVLDIGEFSESLT HygR 70 75 80 85 SAALPIPEVLDIGEFSESLT HygR SAALPIPEVLDIGEFSESLT RGRERNRFHKVNPFEALAQG a t c g g c c g c g c t c c c g a t t c c g g a a g t g c t t g a c a t t g g g g a a t t c a g c g a g a g c c t g a c t a g c c g g c g c g a g g g c t a a g g c c t t c a c g a a c t g t a a c c c c t t a a g t c g c t c t c g g a c t g

3660 90 95 100 105 YCISRRAQGVTLQDLPETEL HygR 90 95 100 105 YCISRRAQGVTLQDLPETEL HygR YCISRRAQGVTLQDLPETEL IADGATCLTDRQLVQRFGFQ c t a t t g c a t c t c c c g c c g t g c a c a g g g t g t c a c g t t g c a a g a c c t g c c t g a a a c c g a a c t g a t a a c g t a g a g g g c g g c a c g t g t c c c a c a g t g c a a c g t t c t g g a c g g a c t t t g g c t t g a

AsiSINcoI 3720 110 115 120 125 PAVLQPVAEAMDAIAAADLS HygR 110 115 120 125 PAVLQPVAEAMDAIAAADLS HygR PAVLQPVAEAMDAIAAADLS GSNQLRDRLGHIRDSRGIKA g c c c g c t g t t c t g c a g c c g g t c g c g g a g g c c a t g g a t g c g a t c g c t g c g g c c g a t c t t a g c g g g c g a c a a g a c g t c g g c c a g c g c c t c c g g t a c c t a c g c t a g c g a c g c c g g c t a g a a t c

RsrII 3780 130 135 140 145 QTSGFGPFGPQGIGQYTTWR HygR 130 135 140 145 QTSGFGPFGPQGIGQYTTWR HygR QTSGFGPFGPQGIGQYTTWR LRAPEAWESRLSDTLVSCPT c c a g a c g a g c g g g t t c g g c c c a t t c g g a c c g c a a g g a a t c g g t c a a t a c a c t a c a t g g c g g g t c t g c t c g c c c a a g c c g g g t a a g c c t g g c g t t c c t t a g c c a g t t a t g t g a t g t a c c g c

NdeI 3840 150 155 160 165 DFICAIADPHVYHWQTVMDD HygR 150 155 160 165 DFICAIADPHVYHWQTVMDD HygR DFICAIADPHVYHWQTVMDD IEYARNSIGMHIVPLSHHVV t g a t t t c a t a t g c g c g a t t g c t g a t c c c c a t g t g t a t c a c t g g c a a a c t g t g a t g g a c g a a c t a a a g t a t a c g c g c t a a c g a c t a g g g g t a c a c a t a g t g a c c g t t t g a c a c t a c c t g c t

3900 170 175 180 185 TVSASVAQALDELMLWAEDC HygR 170 175 180 185 TVSASVAQALDELMLWAEDC HygR TVSASVAQALDELMLWAEDC GDTRGDRLSEILQHKPGLVA c a c c g t c a g t g c g t c c g t c g c g c a g g c t c t c g a t g a g c t g a t g c t t t g g g c c g a g g a c t g g t g g c a g t c a c g c a g g c a g c g c g t c c g a g a g c t a c t c g a c t a c g a a a c c c g g c t c c t g a c

3960 190 195 200 205 PEVRHLVHADFGSNNVLTDN HygR 190 195 200 205 PEVRHLVHADFGSNNVLTDN HygR PEVRHLVHADFGSNNVLTDN GFDPVEHVRIEAGVIDQRVI c c c c g a a g t c c g g c a c c t c g t g c a c g c g g a t t t c g g c t c c a a c a a t g t c c t g a c g g a c a a g g g g c t t c a g g c c g t g g a g c a c g t g c g c c t a a a g c c g a g g t t g t t a c a g g a c t g c c t g t t

4020 210 215 220 225 GRITAVIDWSEAMFGDSQYE HygR 210 215 220 225 GRITAVIDWSEAMFGDSQYE HygR GRITAVIDWSEAMFGDSQYE AAYCRDNVPALRHEPIGLVL t g g c c g c a t a a c a g c g g t c a t t g a c t g g a g c g a g g c g a t g t t c g g g g a t t c c c a a t a c g a a c c g g c g t a t t g t c g c c a g t a a c t g a c c t c g c t c c g c t a c a a g c c c c t a a g g g t t a t g c t

4080 230 235 240 245 VANIFFWRPWLACMEQQTRY HygR 230 235 240 245 VANIFFWRPWLACMEQQTRY HygR VANIFFWRPWLACMEQQTRY DGVDEEPPRPQSTHLLLRAV g g t c g c c a a c a t c t t c t t c t g g a g g c c g t g g t t g g c t t g t a t g g a g c a g c a g a c g c g c t a c c a g c g g t t g t a g a a g a a g a c c t c c g g c a c c a a c c g a a c a t a c c t c g t c g t c t g c g c g a t

4140 250 255 260 265 FERRHPELAGSPRLRAYMLR HygR 250 255 260 265 FERRHPELAGSPRLRAYMLR HygR FERRHPELAGSPRLRAYMLR ELPPM c t t c g a g c g g a g g c a t c c g g a g c t t g c a g g a t c g c c g c g g c t c c g g g c g t a t a t g c t c c g g a a g c t c g c c t c c g t a g g c c t c g a a c g t c c t a g c g g c g c c g a g g c c c g c a t a t a c g a g g c

4200 270 275 280 285 IGLDQLYQSLVDGNFDDAAW HygR 270 275 280 285 IGLDQLYQSLVDGNFDDAAW HygR IGLDQLYQSLVDGNFDDAAW c a t t g g t c t t g a c c a a c t c t a t c a g a g c t t g g t t g a c g g c a a t t t c g a t g a t g c a g c t t g g t a a c c a g a a c t g g t t g a g a t a g t c t c g a a c c a a c t g c c g t t a a a g c t a c t a c g t c g a a c

4260 290 295 300 305 AQGRCDAIVRSGAGTVGRTQ HygR 290 295 300 305 AQGRCDAIVRSGAGTVGRTQ HygR AQGRCDAIVRSGAGTVGRTQ g g c g c a g g g t c g a t g c g a c g c a a t c g t c c g a t c c g g a g c c g g g a c t g t c g g g c g t a c a c a c c g c g t c c c a g c t a c g c t g c g t t a g c a g g c t a g g c c t c g g c c c t g a c a g c c c g c a t g t g t

4320 310 315 320 325 IARRSAAVWTDGCVEVLADS HygR 310 315 320 325 IARRSAAVWTDGCVEVLADS HygR IARRSAAVWTDGCVEVLADS a a t c g c c c g c a g a a g c g c g g c c g t c t g g a c c g a t g g c t g t g t a g a a g t a c t c g c c g a t a g t t a g c g g g c g t c t t c g c g c c g g c a g a c c t g g c t a c c g a c a c a t c t t c a t g a g c g g c t a t c

4380 330 335 340 GNRRPSTRPRAKE* HygR 330 335 340 GNRRPSTRPRAKE* HygR GNRRPSTRPRAKE* t g g a a a c c g a c g c c c c a g c a c t c g t c c g a g g g c a a a g g a a t a g c a c g t a c t a c g a g a t t t a c c t t t g g c t g c g g g g t c g t g a g c a g g c t c c c g t t t c c t t a t c g t g c a t g a t g c t c t a a a

PfoI 4440 c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c

4500 SV40 poly(A) signal c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a

PsiI 4560 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g

BsmI 4620 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a

BstZ17I 4680 M13 rev C A G T A T C G A C A A A G G A C M13 Reverse G G A C A M13/pUC Reverse c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a

4740 -10 -35 lac operator lac promoter C A C T T T A A C A A T A G G C G A M13/pUC Reverse g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t

4800 lac promoter CAP binding site a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g

4860 t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c

4920 G A G C G A G T G A C T G A G C G A L4440 a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a

4980 c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t

5040 a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c

5100 ori t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t

5160 ori T T T C T A T G G T C C G C A A pBR322ori-F a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a

5220 ori A G G G pBR322ori-F t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a

5280 ori g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a

5340 ori c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g

5400 ori c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a

5460 ori a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g

5520 ori t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a

5580 ori c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t

5640 ori a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t

5700 ori a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t

5760 a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a

5820 t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t

5880 285 280 275 270 *WHKILSAGIEAIQRNRED AmpR *WHKILSAGIEAIQRNRED c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g

AhdI 5940 265 260 255 250 MTAQSGTTYIVVIRSPKGDP AmpR MTAQSGTTYIVVIRSPKGDP c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c

6000 245 240 235 230 GLAAIIGRSGREGAGSKDAI AmpR GLAAIIGRSGREGAGSKDAI c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a

6060 225 220 215 210 FWGAPLASRLLPGAVKDAEM AmpR FWGAPLASRLLPGAVKDAEM a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a

6120 205 200 195 190 WDILQQRSALTLLEGTLLKR AmpR WDILQQRSALTLLEGTLLKR c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c

6180 185 180 175 170 LTTAMAVPMTTDREDNPIAE AmpR LTTAMAVPMTTDREDNPIAE c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g

6240 165 160 155 150 NLEPEWRDLRTVHDGMNHLF AmpR NLEPEWRDLRTVHDGMNHLF a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t

6300 145 140 135 130 ATLEKPGGITTLLLNAATND AmpR ATLEKPGGITTLLLNAATND a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g

6360 125 120 115 110 SMTIAASCLERVTMGDTLHK AmpR SMTIAASCLERVTMGDTLHK a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a

6420 105 100 95 90 ETVPSYEVLDNQSYHIRRGL AmpR ETVPSYEVLDNQSYHIRRGL t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c

6480 85 80 75 70 QEQGADIRSLVAGCLLVKFT AmpR A T A A T A C C G C G C C A C A T A G C Amp-R QEQGADIRSLVAGCLLVKFT t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a

6540 65 60 55 50 SMMPFREEPRFSELIKGSNL AmpR SMMPFREEPRFSELIKGSNL g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c

6600 45 40 35 30 DLEIYGVRAGLQDEADKVKV AmpR DLEIYGVRAGLQDEADKVKV a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g

6660 25 LTEPH 20 15 10 signal sequence AFVPLCFAAFFPILA AmpR LTEPHAFVPLCFAAFFPILA c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g

SspI 6720 5 1 signal sequence VRFHQISM AmpR AmpR promoter VRFHQISM g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t

6780 AmpR promoter g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c

SgrDI 6840 AmpR promoter g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g

6900 A T G T T A G A C G A G A C T A C G G C pRS-marker g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a

6960 c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t

7020 a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c

7051 c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.