Addgene pL8_3xG34-DCAF8L2_X1 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pL8_3xG34-DCAF8L2_X1 Sequencing Result


Map View

SgrDI (5722) SspI (5605) Amp-R (5351 .. 5370) ScaI (5281) FspI (5023) AhdI (4801) pBR322ori-F (4049 .. 4068) PciI (3908) L4440 (3798 .. 3815) CAP binding site M13/pUC Reverse (3580 .. 3602) lac operator M13 Reverse (3567 .. 3583) BstZ17I (3529) EBV-rev (3488 .. 3507) BsmI (3477) SV40pA-R (3434 .. 3453) BlpI (3236) BsaBI * (3141) PpuMI (3002) EagI (2959) BbsI (2928) pRS-marker (44 .. 63) MfeI (161) Bpu10I (180) MluI (228) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) CMV-F (769 .. 789) Eco53kI (816) SacI (818) T7 (863 .. 882) NheI (895) BmtI (899) SV40 NLS PstI (975) SV40 NLS c-myc NLS BssHII (1240) SgrAI (1279) AleI (1280) BamHI (1369) BfuAI - BspMI - PaqCI (1370) Bsu36I (1583) PspOMI * (1709) ApaI * (1713) HindIII (1812) BsrGI (1897) F1ori-R (2045 .. 2064) F1ori-F (2255 .. 2276) pBABE 3' (2395 .. 2415) SV40 promoter SV40pro-F (2642 .. 2661) StuI (2713) AvrII (2714) TspMI - XmaI (2735) SmaI (2737) BclI * (2765) MscI (2846) pL8_3xG34-DCAF8L2_X1 5724 bp

Sequence View

5ʹ 3ʹ 60 A T G T T A G A C G A G A C T A C pRS-marker g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c

120 G G C pRS-marker c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c

MfeI 180 c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g

MluIBpu10I 240 CMV enhancer t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a

SpeI 300 CMV enhancer g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t

360 CMV enhancer t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g

420 CMV enhancer c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g

480 CMV enhancer a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a

NdeI 540 CMV enhancer a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a

SnaBI 600 CMV enhancer a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t

660 CMV enhancer CMV promoter t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c

720 CMV promoter a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g

780 CMV promoter C G C A A A T G G G C G CMV-F a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c

Eco53kISacI 840 CMV promoter G T A G G C G T G CMV-F *SSFG g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c t c t g g c t a a c t a g a g a a c c c a c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g a g a c c g a t t g a t c t c t t g g g t

BmtINheI 900 T7 promoter T A A T A C G A C T C A C T A T A G G G T7 SSVPKDFNIRSVIPLGLSAL c t g c t t a c t g g c t t a t c g a a a t t a a t a c g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g c g a c g a a t g a c c g a a t a g c t t t a a t t a t g c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c g

960 130 PKKKRKV SV40 NLS MKRTADGSEFESPKKKRKV VMFRVASPLSNSDGFFFRFT a c c a t g a a a c g g a c a g c c g a c g g a a g c g a g t t c g a g t c a c c a a a g a a g a a g c g g a a a g t c t g g t a c t t t g c c t g t c g g c t g c c t t c g c t c a a g c t c a g t g g t t t c t t c t t c g c c t t t c a g

PstI 1020 SGS 1 5 PAAKRVKLD (in frame with c-myc NLS) SGSKRTAD c-myc NLS SGSPAAKRVKLDSGSKRTAD LPDGAALLTFSSEPEFLVAS a g c g g a t c t c c t g c a g c t a a g a g a g t g a a g c t g g a c t c t g g t t c a a a g a g a a c t g c t g a t t c g c c t a g a g g a c g t c g a t t c t c t c a c t t c g a c c t g a g a c c a a g t t t c t c t t g a c g a c t a

1080 (in frame with c-myc NLS) GSEFES 130 PKKKRKV SGS 1 PAAK SV40 NLS c-myc NLS GSEFESPKKKRKVSGSPAAK PESNSDGLFFLFTEPLGAAF g g t t c t g a a t t t g a a t c t c c t a a a a a a a a a a g a a a g g t t t c a g g c a g c c c a g c t g c a a a a c c a a g a c t t a a a c t t a g a g g a t t t t t t t t t t c t t t c c a a a g t c c g t c g g g t c g a c g t t t t

1140 5 RVKLD (in frame with c-myc NLS) SGSAAMAERPFACDI c-myc NLS RVKLDSGSAAMAERPFACDI LTLNSLPDAAIASLGKAQSI a g g g t t a a a t t g g a t a g t g g g t c c g c c g c t a t g g c t g a g a g g c c c t t c g c c t g c g a t a t a t c c c a a t t t a a c c t a t c a c c c a g g c g g c g a t a c c g a c t c t c c g g g a a g c g g a c g c t a t a t

1200 (in frame with c-myc NLS) CGRKFADCRDLARHIRTHTG CGRKFADCRDLARHIRTHTG HPLFNASQRSRARCMRVWVP t g t g g c a g g a a a t t c g c t g a t t g c c g a g a t c t t g c t c g g c a c a t c c g c a c c c a c a c t g g c a c a c c g t c c t t t a a g c g a c t a a c g g c t c t a g a a c g a g c c g t g t a g g c g t g g g t g t g a c c g

BssHII 1260 (in frame with c-myc NLS) EKPFACDICGRKFARSDNLV EKPFACDICGRKFARSDNLV SFGNAHSMQPRLNARESLRT g a a a a g c c g t t t g c g t g t g a c a t c t g c g g a c g c a a g t t t g c g c g c t c t g a t a a c c t g g t c c t t t t c g g c a a a c g c a c a c t g t a g a c g c c t g c g t t c a a a c g c g c g a g a c t a t t g g a c c a g

SgrAIAleI 1320 (in frame with c-myc NLS) RHIRTHTGEKPFACDICGRK RHIRTHTGEKPFACDICGRK LCILVCVPSFGKAQSMQPLF a g a c a t a t a a g g a c a c a c a c c g g t g a g a a a c c c t t c g c t t g c g a c a t t t g c g g g a g a a a g t c t g t a t a t t c c t g t g t g t g g c c a c t c t t t g g g a a g c g a a c g c t g t a a a c g c c c t c t t t c

BamHIBspMIPaqCIBfuAI 1380 (in frame with c-myc NLS) FARSDELVRHTKIHLRGSGG FARSDELVRHTKIHLRGSGG NARDSSSTRWVLICRRPDPP t t t g c a c g g t c a g a t g a g c t g g t a c g c c a t a c c a a g a t a c a c c t g c g g g g a t c c g g c g g a a a a c g t g c c a g t c t a c t c g a c c a t g c g g t a t g g t t c t a t g t g g a c g c c c c t a g g c c g c c t

1440 (in frame with c-myc NLS) GGSGGSGGSAIPVKRGATGE GGSGGSGGSAIPVKRGATGE PPEPPEPPLAMGTFRPAVPS g g a g g t t c t g g c g g t t c t g g a g g a a g t g c c a t t c c a g t g a a g c g c g g c g c t a c c g g t g a a c c t c c a a g a c c g c c a a g a c c t c c t t c a c g g t a a g g t c a c t t c g c g c c g c g a t g g c c a c t t

1500 (in frame with c-myc NLS) TKVFIGNSNSPKSPTKGGCS TKVFIGNSNSPKSPTKGGCS VLTNIPLLLLGFLGVLPPQE a c c a a a g t g t t t a t c g g t a a c a g c a a c a g c c c g a a g a g c c c g a c c a a a g g c g g t t g c t c t t g g t t t c a c a a a t a g c c a t t g t c g t t g t c g g g c t t c t c g g g c t g g t t t c c g c c a a c g a g a

1560 GGS 5 10 15 TNLSDIIEKETGKQLVI UGI GGSTNLSDIIEKETGKQLVI PPEVLRLSMMSFSVPFCSTM g g c g g c t c a a c t a a t c t g a g c g a c a t c a t t g a g a a g g a g a c t g g g a a a c a g c t g g t c a t t c c g c c g a g t t g a t t a g a c t c g c t g t a g t a a c t c t t c c t c t g a c c c t t t g t c g a c c a g t a a

Bsu36I 1620 20 25 30 35 QESILMLPEEVEEVIGNKPE UGI QESILMLPEEVEEVIGNKPE c a g g a g t c c a t c c t g a t g c t g c c t g a g g a g g t g g a g g a a g t g a t c g g c a a c a a g c c a g a g g t c c t c a g g t a g g a c t a c g a c g g a c t c c t c c a c c t c c t t c a c t a g c c g t t g t t c g g t c t c

1680 40 45 50 55 SDILVHTAYDESTDENVMLL UGI SDILVHTAYDESTDENVMLL t c t g a c a t c c t g g t g c a c a c c g c c t a c g a c g a g t c c a c a g a t g a g a a t g t g a t g c t g c t g a g a c t g t a g g a c c a c g t g t g g c g g a t g c t g c t c a g g t g t c t a c t c t t a c a c t a c g a c g a c

PspOMI*ApaI* 1740 60 65 70 75 TSDAPEYKPWALVIQDSNGE UGI TSDAPEYKPWALVIQDSNGE a c c t c t g a c g c c c c c g a g t a t a a g c c t t g g g c c c t g g t c a t c c a g g a t t c t a a c g g c g a g t g g a g a c t g c g g g g g c t c a t a t t c g g a a c c c g g g a c c a g t a g g t c c t a a g a t t g c c g c t c

1800 80 85 NKIKML* UGI NKIKML* a a t a a g a t c a a g a t g c t g t g a t a a g g g g t c t t t g t c c t c t g t a c t g t c t c t c t c c t t g c c t t a t t c t a g t t c t a c g a c a c t a t t c c c c a g a a a c a g g a g a c a t g a c a g a g a g a g g a a c g g

HindIII 1860 c c t a a c c c a a a a a g c t t c a t t t t t c t g t g t a g g c t g c a c a a g a g c c t t g a t t g a a g a t a t g g a t t g g g t t t t t c g a a g t a a a a a g a c a c a t c c g a c g t g t t c t c g g a a c t a a c t t c t a t a

BsrGI 1920 a t t c t t t c t g a a c a g t a t t t a a g g t t t c c a a t a a a a t g t a c a c c c c t c a g a a c t t c t g a g t a a g a a a g a c t t g t c a t a a a t t c c a a a g g t t a t t t t a c a t g t g g g g a g t c t t g a a g a c t c

1980 f1 ori g c g g a a a g a a c c a g c t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a c g c c t t t c t t g g t c g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t

2040 f1 ori a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c

2100 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t

2160 f1 ori g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g

2220 f1 ori a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a

2280 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t

2340 f1 ori a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g

2400 f1 ori SV40 promoter C C T T A C pBABE 3' t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c

2460 SV40 promoter A C A C A G T C A A T C C C A pBABE 3' t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t

2520 SV40 promoter t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t

2580 SV40 promoter SV40 ori g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t

2640 SV40 promoter SV40 ori t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a

2700 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A G G SV40pro-F t t a t t t a t g c a g a g g c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g a a t a a a t a c g t c t c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c

TspMIXmaISmaIStuIAvrII 2760 SV40 promoter SV40 ori g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c

BclI* 2820 EM7 promoter g a t c t g a t c a g c a c g t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c t a g a c t a g t c g t g c a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t

MscI 2880 EM7 promoter MAKPLSQEESTLI c g a c a a g g t g a g g a a c t a a a c c a t g g c c a a g c c t t t g t c t c a a g a a g a a t c c a c c c t c a t g c t g t t c c a c t c c t t g a t t t g g t a c c g g t t c g g a a a c a g a g t t c t t c t t a g g t g g g a g t a

BbsI 2940 ERATATINSIPISEDYSVAS t g a a a g a g c a a c g g c t a c a a t c a a c a g c a t c c c c a t c t c t g a a g a c t a c a g c g t c g c c a g a c t t t c t c g t t g c c g a t g t t a g t t g t c g t a g g g g t a g a g a c t t c t g a t g t c g c a g c g g t c

EagI 3000 AALSSDGRIFTGVNVYHFTG c g c a g c t c t c t c t a g c g a c g g c c g c a t c t t c a c t g g t g t c a a t g t a t a t c a t t t t a c t g g g c g t c g a g a g a g a t c g c t g c c g g c g t a g a a g t g a c c a c a g t t a c a t a t a g t a a a a t g a c c

PpuMI 3060 GPCAELVVLGTAAAAAAGNL g g g a c c t t g t g c a g a a c t c g t g g t g c t g g g c a c t g c t g c t g c t g c g g c a g c t g g c a a c c t c c c t g g a a c a c g t c t t g a g c a c c a c g a c c c g t g a c g a c g a c g a c g c c g t c g a c c g t t g g a

3120 TCIVAIGNENRGILSPCGRC g a c t t g t a t c g t c g c g a t c g g a a a t g a g a a c a g g g g c a t c t t g a g c c c c t g c g g a c g g t g c t g a a c a t a g c a g c g c t a g c c t t t a c t c t t g t c c c c g t a g a a c t c g g g g a c g c c t g c c a c

BsaBI* 3180 RQVLLDLHPGIKAIVKDSDG c c g a c a g g t g c t t c t c g a t c t g c a t c c t g g g a t c a a a g c c a t a g t g a a g g a c a g t g a t g g g g c t g t c c a c g a a g a g c t a g a c g t a g g a c c c t a g t t t c g g t a t c a c t t c c t g t c a c t a c c

BlpI 3240 QPTAVGIRELLPSVCVGGLS a c a g c c g a c g g c a g t t g g g a t t c g t g a a t t g c t g c c c t c g g t a t g t g t g g g a g g g c t a a g t g t c g g c t g c c g t c a a c c c t a a g c a c t t a a c g a c g g g a g c c a t a c a c a c c c t c c c g a t t c

3300 TSWPRSRTDTCYEISIPPPP c a c t t c g t g g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c g t g a a g c a c c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g

3360 SMKGWASESFSGTPAG* t t c t a t g a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g a a g a t a c t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c

3420 SV40 poly(A) signal c g c g g g g a t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g c g c c c c t a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a

BsmI 3480 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a

BstZ17I 3540 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g

3600 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C M13/pUC Reverse t c t a g c t a g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g a g a t c g a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c

3660 -10 -35 lac operator lac promoter CAP binding site G A M13/pUC Reverse c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t

3720 CAP binding site t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g

3780 c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a

3840 G A G C G A G T G A C T G A G C G A L4440 g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t

3900 g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t

PciI 3960 g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c

4020 ori c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a

4080 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g

4140 ori c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a

4200 ori t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g

4260 ori g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t

4320 ori t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t

4380 ori g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c

4440 ori t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c

4500 ori c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a

4560 ori a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t

4620 g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c

4680 a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t

4740 285 *WHK AmpR *WHK a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t

4800 280 275 270 265 ILSAGIEAIQRNREDMTAQS AmpR ILSAGIEAIQRNREDMTAQS a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g

AhdI 4860 260 255 250 245 GTTYIVVIRSPKGDPGLAAI AmpR GTTYIVVIRSPKGDPGLAAI c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c

4920 240 235 230 225 IGRSGREGAGSKDAIFWGAP AmpR IGRSGREGAGSKDAIFWGAP a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t

4980 220 215 210 205 LASRLLPGAVKDAEMWDILQ AmpR LASRLLPGAVKDAEMWDILQ a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a

FspI 5040 200 195 190 185 QRSALTLLEGTLLKRLTTAM AmpR QRSALTLLEGTLLKRLTTAM t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a

5100 180 175 170 165 AVPMTTDREDNPIAENLEPE AmpR AVPMTTDREDNPIAENLEPE g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g

5160 160 155 150 145 WRDLRTVHDGMNHLFATLEK AmpR WRDLRTVHDGMNHLFATLEK c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g

5220 140 135 130 125 PGGITTLLLNAATNDSMTIA AmpR PGGITTLLLNAATNDSMTIA g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t

5280 120 115 110 105 ASCLERVTMGDTLHKETVPS AmpR ASCLERVTMGDTLHKETVPS g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c

ScaI 5340 100 95 90 85 YEVLDNQSYHIRRGLQEQGA AmpR YEVLDNQSYHIRRGLQEQGA t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c

5400 80 75 70 65 DIRSLVAGCLLVKFTSMMPF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R DIRSLVAGCLLVKFTSMMPF t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t

5460 60 55 50 45 REEPRFSELIKGSNLDLEIY AmpR REEPRFSELIKGSNLDLEIY c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t

5520 40 35 30 25 GVRAGLQDEADKVKVLTEPH AmpR GVRAGLQDEADKVKVLTEPH c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t

5580 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFHQ AmpR AFVPLCFAAFFPILAVRFHQ g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t

SspI 5640 1 signal sequence ISM AmpR AmpR promoter ISM a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c

5700 AmpR promoter a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a

SgrDI 3ʹ 5ʹ 5724 c c c c g a a a a g t g c c a c c t g a c g t c g g g g c t t t t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.