Addgene pLenti PGK Blast V5-LUC (w528-1) Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pLenti PGK Blast V5-LUC (w528-1) Sequencing Result


Map View

TspMI - XmaI (9431) PmlI (9396) BsaBI * (9029) KpnI (8913) Acc65I (8909) BmtI (8835) NheI (8831) SV40pA-R (8482 .. 8501) EBV-rev (8428 .. 8447) SfiI (8292) T7 promoter T7 (8203 .. 8222) M13 fwd M13 Forward (8176 .. 8193) M13/pUC Forward (8162 .. 8184) F1ori-R (7929 .. 7948) DraIII (7804) F1ori-F (7717 .. 7738) Amp-R (7212 .. 7231) pBR322ori-F (5910 .. 5929) PciI (5769) L4440 (5659 .. 5676) CAP binding site lac promoter M13/pUC Reverse (5441 .. 5463) lac operator M13 Reverse (5428 .. 5444) M13 rev T3 (5389 .. 5409) T3 promoter SmaI (9433) CsiI - SexAI * (234) pBABE 3' (306 .. 326) SacII (463) PflMI (726) WPRE-R (892 .. 912) XbaI (1020) SgrAI (1246) PaqCI (1285) BstBI (2507) LucNrev (2595 .. 2613) attB1 SalI (2865) AgeI (2873) hPGK-F (2950 .. 2968) AscI (3047) BsmBI - Esp3I (3136) PaeR7I - XhoI (3419) BspDI * - ClaI * (3438) HpaI (3563) KflI (3654) AleI (4014) BbvCI - Bpu10I (4164) pLenti PGK Blast V5-LUC (w528-1) 9450 bp

Sequence View

5ʹ 3ʹ 60 SV40 promoter SV40 ori g c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c t c a g a g g c a g a g g c g g c c g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c g a g t c t c c g t c t c c g c c g

120 SV40 promoter SV40 ori c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g c g g a a c t g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c g c c t t g a

180 SV40 promoter SV40 ori g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a c t a t g g t t g c t g a c t a a t t c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t g a t a c c a a c g a c t g a t t a a

SexAI*CsiI 240 SV40 promoter g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t t t c c a c a c c t g g t c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a a a g g t g t g g a c c a

300 SV40 promoter t g c t g a c t a a t t g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t t a c g a c t g a t t a a c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a a

360 SV40 promoter A C C C T A A C T G A C A C A C A T T C C pBABE 3' *GS t c c a c a c c c t a a c t g a c a c a c a t t c c a c a g a a t t a a t t c c a a a c t c a t t a c t a a c c g g a t a g g t g t g g g a t t g a c t g t g t g t a a g g t g t c t t a a t t a a g g t t t g a g t a a t g a t t g g c c t a

420 WPRE IQLIGPPSAAWLSIRSTQPR a t c t g c a g a a t t c c a g g c g g g g a g g c g g c c c a a a g g g a g a t c c g a c t c g t c t g a g g g c g a t a g a c g t c t t a a g g t c c g c c c c t c c g c c g g g t t t c c c t c t a g g c t g a g c a g a c t c c c g c t

SacII 480 WPRE LRLRPLPRLAPLLGRSPLDA a g g c g a a g a c g c g g a a g a g g c c g c a g a g c c g g c a g c a g g c c g c g g g a a g g a a g g t c c g c t t c c g c t t c t g c g c c t t c t c c g g c g t c t c g g c c g t c g t c c g g c g c c c t t c c t t c c a g g c g a

540 WPRE 1 IEGR (in frame with Factor Xa site) RDVAEGRPAQNPGGN Factor Xa site PNLASPVYCFSTGRLIWTAV g g a t t g a g g g c c g a a g g g a c g t a g c a g a a g g a c g t c c c g c g c a g a a t c c a g g t g g c a a c a c c t a a c t c c c g g c t t c c c t g c a t c g t c t t c c t g c a g g g c g c g t c t t a g g t c c a c c g t t g t

600 WPRE (in frame with Factor Xa site) TGEQPWKGRQLPRQHHGIVS CALLWPFSTLKGSLVVSNDT c a g g c g a g c a g c c a t g g a a a g g a c g t c a g c t t c c c c g a c a a c a c c a c g g a a t t g t c a g t g g t c c g c t c g t c g g t a c c t t t c c t g c a g t c g a a g g g g c t g t t g t g g t g c c t t a a c a g t c a c

660 WPRE (in frame with Factor Xa site) AQQPSPCPAAGKAGGDEFRR GLLRAGTWCRALCAAILEAT c c c a a c a g c c g a g c c c c t g t c c a g c a g c g g g c a a g g c a g g c g g c g a t g a g t t c c g c c g t g g g g t t g t c g g c t c g g g g a c a g g t c g t c g c c c g t t c c g t c c g c c g c t a c t c a a g g c g g c a c

720 WPRE (in frame with Factor Xa site) GNREGESESPGKELTGGGNA AIPLPFAFTGSLLQCTTAIG g c a a t a g g g a g g g g g a a a g c g a a a g t c c c g g a a a g g a g c t g a c a g g t g g t g g c a a t g c c c c g t t a t c c c t c c c c c t t t c g c t t t c a g g g c c t t t c c t c g a c t g t c c a c c a c c g t t a c g g g

PflMI 780 WPRE (in frame with Factor Xa site) PTSGGCVSKHSAHHATLPDN WGTPTADAFVTCVVGRQRVV c a a c c a g t g g g g g t t g c g t c a g c a a a c a c a g t g c a c a c c a c g c c a c g t t g c c t g a c a a c g g t t g g t c a c c c c c a a c g c a g t c g t t t g t g t c a c g t g t g g t g c g g t g c a a c g g a c t g t t g c

840 WPRE (in frame with Factor Xa site) GPQLLIKRQQPGFIQGGENE PWLEEYLSLLWSKYLSSFIF g g c c a c a a c t c c t c a t a a a g a g a c a g c a a c c a g g a t t t a t a c a a g g a g g a g a a a a t g a a a c c g g t g t t g a g g a g t a t t t c t c t g t c g t t g g t c c t a a a t a t g t t c c t c c t c t t t t a c t t t

900 WPRE (in frame with Factor Xa site) SHTGSNSMIQRH* C A T A G C G T A WPRE-R AMRSAIAHYLPM g c c a t a c g g g a a g c a a t a g c a t g a t a c a a a g g c a t t a a a g c a g c g t a t c c a c a t a g c g t a c g g t a t g c c c t t c g t t a t c g t a c t a t g t t t c c g t a a t t t c g t c g c a t a g g t g t a t c g c a t

960 WPRE A A A G G A G C A A C A WPRE-R a a a g g a g c a a c a t a g t t a a g a a t a c c a g t c a a t c t t t c a c a a a t t t t g t a a t c c a g a g g t t t t c c t c g t t g t a t c a a t t c t t a t g g t c a g t t a g a a a g t g t t t a a a a c a t t a g g t c t c c a

1020 WPRE attB2 t g a t t g t c g a g c g g c c g c c a c t g t g c t g g a t a t c a a c c a c t t t g t a c a a g a a a g c t g g g t a c t a a c a g c t c g c c g g c g g t g a c a c g a c c t a t a g t t g g t g a a a c a t g t t c t t t c g a c c c a

XbaI 1080 550 545 540 535 *VAIKGGKKAKILIERIK luciferase *VAIKGGKKAKILIERIK c t a g a a t t a c a c g g c g a t c t t t c c g c c c t t c t t g g c c t t t a t g a g g a t c t c t c t g a t t t t g a t c t t a a t g t g c c g c t a g a a a g g c g g g a a g a a c c g g a a a t a c t c c t a g a g a g a c t a a a a

1140 530 525 520 515 RADLKGTLGKPVEDVFVVGG luciferase RADLKGTLGKPVEDVFVVGG t c t t g c g t c g a g t t t t c c g g t a a g a c c t t t c g g t a c t t c g t c c a c a a a c a c a a c t c c t c c a g a a c g c a g c t c a a a a g g c c a t t c t g g a a a g c c a t g a a g c a g g t g t t t g t g t t g a g g a g g

1200 510 505 500 495 RLKKATTVQSAVYDVIEKET luciferase RLKKATTVQSAVYDVIEKET g c g c a a c t t t t t c g c g g t t g t t a c t t g a c t g g c g a c g t a a t c c a c g a t c t c t t t t t c c g t c g c g t t g a a a a a g c g c c a a c a a t g a a c t g a c c g c t g c a t t a g g t g c t a g a g a a a a a g g c a

SgrAI 1260 490 485 480 475 MTKGHELVVVAAPLEGADDD luciferase MTKGHELVVVAAPLEGADDD c a t c g t c t t t c c g t g c t c c a a a a c a a c a a c g g c g g c g g g a a g t t c a c c g g c g t c a t c g t c g t a g c a g a a a g g c a c g a g g t t t t g t t g t t g c c g c c g c c c t t c a a g t g g c c g c a g t a g c a g

PaqCI 1320 470 465 460 455 PLGAVGADFINPHQLLISEL luciferase PLGAVGADFINPHQLLISEL g g g a a g a c c t g c g a c a c c t g c g t c g a a g a t g t t g g g g t g t t g g a g c a a g a t g g a t t c c a a c c c t t c t g g a c g c t g t g g a c g c a g c t t c t a c a a c c c c a c a a c c t c g t t c t a c c t a a g g t t

1380 450 445 440 435 EAPAVQYGKYKILSKLRDVI luciferase EAPAVQYGKYKILSKLRDVI t t c a g c g g g a g c c a c c t g a t a g c c t t t g t a c t t a a t c a g a g a c t t c a g g c g g t c a a c g a t a a g t c g c c c t c g g t g g a c t a t c g g a a a c a t g a a t t a g t c t c t g a a g t c c g c c a g t t g c t a

1440 430 425 420 415 FFHEDEDWYAIDGSHLWGDK luciferase FFHEDEDWYAIDGSHLWGDK g a a g a a g t g t t c g t c t t c g t c c c a g t a a g c t a t g t c t c c a g a a t g t a g c c a t c c a t c c t t c t t c t t c a c a a g c a g a a g c a g g g t c a t t c g a t a c a g a g g t c t t a c a t c g g t a g g t a g g a a

1500 410 405 400 395 DILANTAEPNNVYGSMIMPG luciferase DILANTAEPNNVYGSMIMPG g t c a a t c a a g g c g t t g g t c g c t t c c g g a t t g t t t a c a t a a c c g g a c a t a a t c a t a g g a c c c a g t t a g t t c c g c a a c c a g c g a a g g c c t a a c a a a t g t a t t g g c c t g t a t t a g t a t c c t g g

1560 390 385 380 375 RVCLEGRQNVGLTKGTDLDV luciferase RVCLEGRQNVGLTKGTDLDV t c t c a c a c a c a g t t c g c c t c t t t g a t t a a c g c c c a g c g t t t t c c c g g t a t c c a g a t c c a c a g a g t g t g t g t c a a g c g g a g a a a c t a a t t g c g g g t c g c a a a a g g g c c a t a g g t c t a g g t g

1620 370 365 360 355 VKAEFFPVVKGVAGPKDDGE luciferase VKAEFFPVVKGVAGPKDDGE a a c c t t c g c t t c a a a a a a t g g a a c a a c t t t a c c g a c c g c g c c c g g t t t a t c a t c c c c c t c t t g g a a g c g a a g t t t t t t a c c t t g t t g a a a t g g c t g g c g c g g g c c a a a t a g t a g g g g g a g

1680 350 345 340 335 PTILIASTTETLGYGQRIGP luciferase PTILIASTTETLGYGQRIGP g g g t g t a a t c a g a a t a g c t g a t g t a g t c t c a g t g a g c c c a t a t c c t t g c c t g a t a c c t g g c c c a c a t t a g t c t t a t c g a c t a c a t c a g a g t c a c t c g g g t a t a g g a a c g g a c t a t g g a c c

1740 330 325 320 315 LHFRKAVAEGVEKSLPAGGS luciferase LHFRKAVAEGVEKSLPAGGS c a g a t g g a a c c t c t t g g c a a c c g c t t c c c c g a c t t c c t t a g a g a g g g g a g c g c c a c c a g a g t c t a c c t t g g a g a a c c g t t g g c g a a g g g g c t g a a g g a a t c t c t c c c c t c g c g g t g g t c t

1800 310 305 300 295 AIEHLNSLDYKDILTSKAFF luciferase AIEHLNSLDYKDILTSKAFF a g c a a t t t c g t g t a a a t t a g a t a a a t c g t a t t t g t c a a t c a g a g t g c t t t t g g c g a a g a a t c g t t a a a g c a c a t t t a a t c t a t t t a g c a t a a a c a g t t a g t c t c a c g a a a a c c g c t t c t t

1860 290 285 280 275 SFLTPVLLASQIKYDQLSRL luciferase SFLTPVLLASQIKYDQLSRL g g a g a a t a g g g t t g g c a c c a g c a g c g c a c t t t g a a t c t t g t a a t c c t g a a g g c t c c t c a g c c t c t t a t c c c a a c c g t g g t c g t c g c g t g a a a c t t a g a a c a t t a g g a c t t c c g a g g a g t c

1920 270 265 260 255 FLEEEFRYMLVVRFGCILYG luciferase FLEEEFRYMLVVRFGCILYG a a a c a g c t c t t c t t c a a a t c t a t a c a t t a a g a c g a c t c g a a a t c c a c a t a t c a a a t a t c c t t t g t c g a g a a g a a g t t t a g a t a t g t a a t t c t g c t g a g c t t t a g g t g t a t a g t t t a t a g g

1980 250 245 240 235 LTTFMGFGHHFPVVSLIATD luciferase LTTFMGFGHHFPVVSLIATD g a g t g t a g t a a a c a t t c c a a a a c c g t g a t g g a a t g g a a c a a c a c t t a a a a t c g c a g t a t c c t c a c a t c a t t t g t a a g g t t t t g g c a c t a c c t t a c c t t g t t g t g a a t t t t a g c g t c a t a g

2040 230 225 220 215 PIIQNGFIPDRAHSFRVCAT luciferase PIIQNGFIPDRAHSFRVCAT c g g a a t g a t t t g a t t g c c a a a a a t a g g a t c t c t g g c a t g c g a g a a t c t c a c g c a g g c a g t g c c t t a c t a a a c t a a c g g t t t t t a t c c t a g a g a c c g t a c g c t c t t a g a g t g c g t c c g t c a

2100 210 205 200 195 RHPLAVGKPLGTSGSSNMIL luciferase RHPLAVGKPLGTSGSSNMIL t c t a t g a g g c a g a g c g a c a c c t t t a g g c a g a c c a g t a g a t c c a g a g g a g t t c a t g a t c a g a g a t a c t c c g t c t c g c t g t g g a a a t c c g t c t g g t c a t c t a g g t c t c c t c a a g t a c t a g t c

2160 190 185 180 175 AITKDRDFSEPVFDYENFGP luciferase AITKDRDFSEPVFDYENFGP t g c a a t t g t c t t g t c c c t a t c g a a g g a c t c t g g c a c a a a a t c g t a t t c a t t a a a a c c g g g a c g t t a a c a g a a c a g g g a t a g c t t c c t g a g a c c g t g t t t t a g c a t a a g t a a t t t t g g c c c

2220 170 165 160 155 PLHSTVFTYMSQFGQYDTKS luciferase PLHSTVFTYMSQFGQYDTKS a g g t a g a t g a g a t g t g a c g a a c g t g t a c a t c g a c t g a a a t c c c t g g t a a t c c g t t t t a g a t c c a t c t a c t c t a c a c t g c t t g c a c a t g t a g c t g a c t t t a g g g a c c a t t a g g c a a a a t c t

2280 150 145 140 135 DMIIIKQIIPLKKQVNLIKQ luciferase DMIIIKQIIPLKKQVNLIKQ a t c c a t g a t a a t a a t t t t t t g g a t g a t t g g g a g c t t t t t t t g c a c g t t c a a a a t t t t t t g t a g g t a c t a t t a t t a a a a a a c c t a c t a a c c c t c g a a a a a a a c g t g c a a g t t t t a a a a a a c

2340 130 125 120 115 LGKKSVFVVTPQSIGMSNLL luciferase LGKKSVFVVTPQSIGMSNLL c a a c c c c t t t t t g g a a a c g a a c a c c a c g g t a g g c t g c g a a a t g c c c a t a c t g t t g a g c a a g t t g g g g a a a a a c c t t t g c t t g t g g t g c c a t c c g a c g c t t t a c g g g t a t g a c a a c t c g t t

2400 110 105 100 95 ERENYIDNAPAVAVGIFLAG luciferase ERENYIDNAPAVAVGIFLAG t t c a c g t t c a t t a t a a a t g t c g t t c g c g g g c g c a a c t g c a a c t c c g a t a a a t a a c g c g c c a a g t g c a a g t a a t a t t t a c a g c a a g c g c c c g c g t t g a c g t t g a g g c t a t t t a t t g c g c g g

2460 90 85 80 75 LVPMFFQLSNESCVVIRHNT luciferase LVPMFFQLSNESCVVIRHNT c a a c a c c g g c a t a a a g a a t t g a a g a g a g t t t t c a c t g c a t a c g a c g a t t c t g t g a t t t g t g t t g t g g c c g t a t t t c t t a a c t t c t c t c a a a a g t g a c g t a t g c t g c t a a g a c a c t a a a c a

BstBI 2520 70 65 60 55 NLGYRKMAEALRVSMEFYEA luciferase NLGYRKMAEALRVSMEFYEA a t t c a g c c c a t a t c g t t t c a t a g c t t c t g c c a a c c g a a c g g a c a t t t c g a a g t a c t c a g c t a a g t c g g g t a t a g c a a a g t a t c g a a g a c g g t t g g c t t g c c t g t a a a g c t t c a t g a g t c g

2580 50 45 40 35 YTIDVEIHADTFAITGPVLA luciferase YTIDVEIHADTFAITGPVLA g t a a g t g a t g t c c a c c t c g a t a t g t g c a t c t g t a a a a g c a a t t g t t c c a g g a a c c a g g g c c a t t c a c t a c a g g t g g a g c t a t a c a c g t a g a c a t t t t c g t t a a c a a g g t c c t t g g t c c c g

2640 30 25 20 15 YRKMAKHLQEGATGDELPYF luciferase C C T T A T G C A G T T G C T C T C C LucNrev YRKMAKHLQEGATGDELPYF g t a t c t c t t c a t a g c c t t a t g c a g t t g c t c t c c a g c g g t t c c a t c t t c c a g c g g a t a g a a c a t a g a g a a g t a t c g g a a t a c g t c a a c g a g a g g t c g c c a a g g t a g a a g g t c g c c t a t c t t

2700 (in frame with V5 tag) TAKGVTG 10 5 1 PAPGKKINKADEM luciferase Kozak sequence MFLASSMVALPTVP PAPGKKINKADEMTAKGVTG t g g c g c c g g g c c t t t c t t t a t g t t t t t g g c g t c t t c c a t g g t g g c t t t a c c a a c a g t a c c a c c g c g g c c c g g a a a g a a a t a c a a a a a c c g c a g a a g g t a c c a c c g a a a t g g t t g t c a t g g

2760 (in frame with V5 tag) SHWAKQLLRPVSNTGMSG TS V5 tag ECQAFCKSLGTELVPMDPVE SHWAKQLLRPVSNTGMSGTS g g a a t g c c a a g c t t t t t g c a a a a g c c t a g g g a c t g a a t t g g t t c c c a t g g a t c c c g t a g a c c t t a c g g t t c g a a a a a c g t t t t c g g a t c c c t g a c t t a a c c a a g g g t a c c t a g g g c a t c t

2820 10 5 1 DLGLLPNPIPKG V5 tag attB1 SRPRRGLGIGLPSMVEPAFL DLGLLPNPIPKGEMTSGAKK a t c g a g a c c g a g g a g a g g g t t a g g g a t a g g c t t a c c t t c c a t g g t g g a g c c t g c t t t t t t t a g c t c t g g c t c c t c t c c c a a t c c c t a t c c g a a t g g a a g g t a c c a c c t c g g a c g a a a a a a

AgeISalI 2880 attB1 YKLVDICRIPPHWTSRRTGG YLSTSIQLIGGCQVLRRVPP g t a c a a a c t t g t t g a t a t c t g c a g a a t t c c a c c a c a c t g g a c t a g t c g a c g a a c c g g t g g c a t g t t t g a a c a a c t a t a g a c g t c t t a a g g t g g t g t g a c c t g a t c a g c t g c t t g g c c a c c

2940 hPGK promoter SPWGERSVIRSTREPTADVR DGQPSLDTIRDVLSGVASTR a t c c c c c t g g g g a g a g a g g t c g g t g a t t c g g t c a a c g a g g g a g c c g a c t g c c g a c g t g c g t a g g g g g a c c c c t c t c t c c a g c c a c t a a g c c a g t t g c t c c c t c g g c t g a c g g c t g c a c g c

3000 hPGK promoter G A A C G T C T T A C G C C T T G T G hPGK-F SGGLQNAEHRAGRNRAHTTA EPPKCFASCRAPLFLAWVVA c t c c g g a g g c t t g c a g a a t g c g g a a c a c c g c g c g g g c a g g a a c a g g g c c c a c a c t a c c g c g a g g c c t c c g a a c g t c t t a c g c c t t g t g g c g c g c c c g t c c t t g t c c c g g g t g t g a t g g c g

AscI 3060 hPGK promoter PHPASRTAPSRPLLSARPAE GCGAERVAGERGSSEARGAS c c c a c a c c c c g c c t c c c g c a c c g c c c c t t c c c g g c c g c t g c t c t c g g c g c g c c c t g c t g a g g g t g t g g g g c g g a g g g c g t g g c g g g g a a g g g c c g g c g a c g a g a g c c g c g c g g g a c g a c t

3120 hPGK promoter QPLLATAHRGRRAAIAPWRC CGSNAVAWRPRRAAM g c a g c c g c t a t t g g c c a c a g c c c a t c g c g g t c g g c g c g c t g c c a t t g c t c c c t g g c g c t g c g t c g g c g a t a a c c g g t g t c g g g t a g c g c c a g c c g c g c g a c g g t a a c g a g g g a c c g c g a c

BsmBIEsp3I 3180 (in frame with gp41 peptide) *HSTRSGNTVDPVGR hPGK promoter PSARVLVRRAASVCHVRHAA *HSTRSGNTVDPVGR t c c g t c t g c g a g g g t a c t a g t g a g a c g t g c g g c t t c c g t t t g t c a c g t c c g g c a c g c c g c a g g c a g a c g c t c c c a t g a t c a c t c t g c a c g c c g a a g g c a a a c a g t g c a g g c c g t g c g g c g

3240 (in frame with gp41 peptide) VALFRGSKPASCSADGPPGC hPGK promoter NRKEPSRLRGGAGSVAGGPT VALFRGSKPASCSADGPPGC g a a c c g c a a g g a a c c t t c c c g a c t t a g g g g c g g a g c a g g a a g c g t c g c c g g g g g g c c c a c c t t g g c g t t c c t t g g a a g g g c t g a a t c c c c g c c t c g t c c t t c g c a g c g g c c c c c c g g g t g

3300 (in frame with gp41 peptide) PYRRLDPHRQSRVHLIHSVW hPGK promoter RVAAKIRVTLRTDVKNVRDP PYRRLDPHRQSRVHLIHSVW a a g g g t a g c g g c g a a g a t c c g g g t g a c g c t g c g a a c g g a c g t g a a g a a t g t g c g a g a c c c t t c c c a t c g c c g c t t c t a g g c c c a c t g c g a c g c t t g c c t g c a c t t c t t a c a c g c t c t g g g

3360 (in frame with gp41 peptide) PRRRQTERFWAWLLRTGACV hPGK promoter GSAPLRFPEPRPEQPRPCAN PRRRQTERFWAWLLRTGACV a g g g t c g g c g c c g c t g c g t t t c c c g g a a c c a c g c c c a g a g c a g c c g c g t c c c t g c g c a a a t c c c a g c c g c g g c g a c g c a a a g g g c c t t g g t g c g g g t c t c g t c g g c g c a g g g a c g c g t t t

PaeR7IXhoI 3420 (in frame with gp41 peptide) WPQRPFPAVGVGHFEIDLKE hPGK promoter PGLPWKRRNPNPVEFDIKLL WPQRPFPAVGVGHFEIDLKE c c c a g g g c t g c c t t g g a a a a g g c g c a a c c c c a a c c c c g t g g a a t t c g a t a t c a a g c t t c t g g g t c c c g a c g g a a c c t t t t c c g c g t t g g g g t t g g g g c a c c t t a a g c t a t a g t t c g a a g a

BspDI*ClaI* 3480 (in frame with gp41 peptide) LSTEHDIFNQIKTIQKQLEK cPPT/CTS EASLVIDKILNFCNLFL* LSTEHDIFNQIKTIQKQLEK c g a g g c t a g t c t c g t g a t c g a t a a a a t t t t g a a t t t t t g t a a t t t g t t t t t g t a a t t c t t g c t c c g a t c a g a g c a c t a g c t a t t t t a a a a c t t a a a a a c a t t a a a c a a a a a c a t t a a g a a

3540 (in frame with gp41 peptide) TQIDTAIIDVIREGASYGGI cPPT/CTS TQIDTAIIDVIREGASYGGI t a g t t t g t a t g t c t g t t g c t a t t a t g t c t a c t a t t c t t t c c c c t g c a c t g t a c c c c c c a a a t c a a a c a t a c a g a c a a c g a t a a t a c a g a t g a t a a g a a a g g g g a c g t g a c a t g g g g g g t t

HpaI 3600 (in frame with gp41 peptide) GGKRKFTLRYRRSGNVLRIS cPPT/CTS GGKRKFTLRYRRSGNVLRIS t c c c c c c t t t t c t t t t a a a a g t t a a c c g a t a c c g t c g a g a t c c g t t c a c t a a t c g a a t g g a g g g g g g a a a a g a a a a t t t t c a a t t g g c t a t g g c a g c t c t a g g c a a g t g a t t a g c t t a c c

KflI 3660 (in frame with gp41 peptide) RDRDREGGEEEIGEPRDPGR RDRDREGGEEEIGEPRDPGR a t c t g t c t c t g t c t c t c t c t c c a c c t t c t t c t t c t a t t c c t t c g g g c c t g t c g g g t c c c c t a g a c a g a g a c a g a g a g a g a g g t g g a a g a a g a a g a t a a g g a a g c c c g g a c a g c c c a g g g g

3720 (in frame with gp41 peptide) PTPLHTQFSLPSYGQRVRNV PTPLHTQFSLPSYGQRVRNV t c g g g g t t g g g a g g t g g g t c t g a a a c g a t a a t g g t g a a t a t c c c t g c c t a a c t c t a t t c a a g c c c c a a c c c t c c a c c c a g a c t t t g c t a t t a c c a c t t a t a g g g a c g g a t t g a g a t a a g t

3780 (in frame with gp41 peptide) ISLVAFVIRLGVLGGVIMIF ISLVAFVIRLGVLGGVIMIF c t a t a g a a a g t a c a g c a a a a a c t a t t c t t a a a c c t a c c a a g c c t c c t a c t a t c a t t a t g a g a t a t c t t t c a t g t c g t t t t t g a t a a g a a t t t g g a t g g t t c g g a g g a t g a t a g t a a t a c t

3840 (in frame with gp41 peptide) LKIYWLWNTINFWNW 15 LSAWK gp41 peptide LKIYWLWNTINFWNWLSAWK a t a a t t t t a t a t a c c a c a g c c a a t t t g t t a t g t t a a a c c a a t t c c a c a a a c t t g c c c a t t t a t t a a a a t a t a t g g t g t c g g t t a a a c a a t a c a a t t t g g t t a a g g t g t t t g a a c g g g t a a

3900 10 5 1 DLELLEQENK gp41 peptide DLELLEQENKEQQNQSEEIL t a t c t a a t t c c a a t a a t t c t t g t t c a t t c t t t t c t t g c t g g t t t t g c g a t t c t t c a a t t a a t a g a t t a a g g t t a t t a a g a a c a a g t a a g a a a a g a a c g a c c a a a a c g c t a a g a a g t t a a t

3960 SHILSTYNNIERDWEMWTTH a g g a g t g t a t t a a g c t t g t g t a a t t g t t a a t t t c t c t g t c c c a c t c c a t c c a g g t c g t g t t c c t c a c a t a a t t c g a a c a c a t t a a c a a t t a a a g a g a c a g g g t g a g g t a g g t c c a g c a c a

AleI 4020 NWIQELSKNSWSANWPVATT g a t t c c a a a t c t g t t c c a g a g a t t t a t t a c t c c a a c t a g c a t t c c a a g g c a c a g c a g t g g c t a a g g t t t a g a c a a g g t c t c t a a a t a a t g a g g t t g a t c g t a a g g t t c c g t g t c g t c a c c

4080 RRE CILKGSCGWIGLLQQDKLYR t g c a a a t g a g t t t t c c a g a g c a a c c c c a a a t c c c c a g g a g c t g t t g a t c c t t t a g g t a t c a c g t t t a c t c a a a a g g t c t c g t t g g g g t t t a g g g g t c c t c g a c a a c t a g g a a a t c c a t a g

4140 RRE EVALIRAQLQKIGWVTLQLL t t t c c a c a g c c a g g a t t c t t g c c t g g a g c t g c t t g a t g c c c c a g a c t g t g a g t t g c a a c a a a a g g t g t c g g t c c t a a g a a c g g a c c t c g a c g a a c t a c g g g g t c t g a c a c t c a a c g t t g t

Bpu10IBbvCI 4200 RRE HQQAEIARLLNNQQQVIGSL g a t g c t g t t g c g c c t c a a t a g c c c t c a g c a a a t t g t t c t g c t g c t g c a c t a t a c c a g a c a c t a c g a c a a c g c g g a g t t a t c g g g a g t c g t t t a a c a a g a c g a c g a c g t g a t a t g g t c t g t

4260 RRE LQRAQVTLTMSAAGMTSGAA a t a a t t g t c t g g c c t g t a c c g t c a g c g t c a t t g a c g c t g c g c c c a t a g t g c t t c c t g c t g t a t t a a c a g a c c g g a c a t g g c a g t c g c a g t a a c t g c g a c g c g g g t a t c a c g a a g g a c g a c

4320 RRE GLFGLFLAGIGVARKERQVV c t c c c a a g a a c c c a a g g a a c a a a g c t c c t a t t c c c a c t g c t c t t t t t t c t c t c t g c a c c a g a g g g t t c t t g g g t t c c t t g t t t c g a g g a t a a g g g t g a c g a g a a a a a a g a g a g a c g t g g t

4380 RRKAKTPAVGLPEIKVVKYK c t c t t c t c t t t g c c t t g g t g g g t g c t a c t c c t a a t g g t t c a a t t t t t a c t a c t t t a t a t t g a g a a g a g a a a c g g a a c c a c c c a c g a t g a g g a t t a c c a a g t t a a a a a t g a t g a a a t a t a a

4440 YLESRWNDRM t a t a t a a t t c a c t t c t c c a a t t g t c c c t c a t a t c t c c t c c t c c a g g t c t g a a g a t c a g c g a t a t a t t a a g t g a a g a g g t t a a c a g g g a g t a t a g a g g a g g a g g t c c a g a c t t c t a g t c g c

4500 g c c g c t t g c t g t g c g g t g g t c t t a c t t t t g t t t t g c t c t t c c t c t a t c t t g t c t a a a g c t c g g c g a a c g a c a c g c c a c c a g a a t g a a a a c a a a a c g a g a a g g a g a t a g a a c a g a t t t c g a

4560 t c c t t g g t g t c t t t t a t c t c t a t c c t t t g a t g c a c a c a a t a g a g g g t t g c t a c t g t a t t a a g g a a c c a c a g a a a a t a g a g a t a g g a a a c t a c g t g t g t t a t c t c c c a a c g a t g a c a t a a t

4620 t a t a a t g a t c t a a g t t c t t c t g a t c c t g t c t g a a g g g a t g g t t g t a g c t g t c c c a g t a t t a t a t t a c t a g a t t c a a g a a g a c t a g g a c a g a c t t c c c t a c c a a c a t c g a c a g g g t c a t a a

4680 t g t c t a c a g c c t t c t g a t g t t t c t a a c a g g c c a g g a t t a a c t g c g a a t c g t t c t a g c t c c a c a g a t g t c g g a a g a c t a c a a a g a t t g t c c g g t c c t a a t t g a c g c t t a g c a a g a t c g a g g

4740 c t g c t t g c c c a t a c t a t a t g t t t t a a t t t a t a t t t t t t c t t t c c c c c t g g c c t t a a c c g a g a c g a a c g g g t a t g a t a t a c a a a a t t a a a t a t a a a a a a g a a a g g g g g a c c g g a a t t g g c t

4800 HIV-1 Ψ a t t t t t t c c c a t c g c g a t c t a a t t c t c c c c c g c t t a a t a c t g a c g c t c t c g c a c c c a t c t t a a a a a a g g g t a g c g c t a g a t t a a g a g g g g g c g a a t t a t g a c t g c g a g a g c g t g g g t a g a

4860 HIV-1 Ψ c t c t c c t t c t a g c c t c c g c t a g t c a a a a t t t t t g g c g t a c t c a c c a g t c g c c g c c c c t c g g a g a g g a a g a t c g g a g g c g a t c a g t t t t a a a a a c c g c a t g a g t g g t c a g c g g c g g g g a g c

4920 HIV-1 Ψ c c t c t t g c c g t g c g c g c t t c a g c a a g c c g a g t c c t g c g t c g a g a g a g c t c c t c t g g t t t c g g a g a a c g g c a c g c g c g a a g t c g t t c g g c t c a g g a c g c a g c t c t c t c g a g g a g a c c a a a g

4980 5' LTR (truncated) c c t t t c g c t t t c a a g t c c c t g t t c g g g c g c c a c t g c t a g a g a t t t t c c a c a c t g a c t a a a g g a a a g c g a a a g t t c a g g g a c a a g c c c g c g g t g a c g a t c t c t a a a a g g t g t g a c t g a t t t

5040 5' LTR (truncated) a g g g t c t g a g g g a t c t c t a g t t a c c a g a g t c a c a c a a c a g a c g g g c a c a c a c t a c t t g a a t c c c a g a c t c c c t a g a g a t c a a t g g t c t c a g t g t g t t g t c t g c c c g t g t g t g a t g a a c t t

5100 5' LTR (truncated) g c a c t c a a g g c a a g c t t t a t t g a g g c t t a a g c a g t g g g t t c c c t a g t t a g c c a g a g a g c t c g t g a g t t c c g t t c g a a a t a a c t c c g a a t t c g t c a c c c a a g g g a t c a a t c g g t c t c t c g a

5160 5' LTR (truncated) RSV promoter c c c a g g c t c a g a t c t g g t c t a a c c a g a g a g a c c c g t t t a t g t a t c g a g c t a g g c a c t t a a g g g t c c g a g t c t a g a c c a g a t t g g t c t c t c t g g g c a a a t a c a t a g c t c g a t c c g t g a a t t

5220 RSV promoter a t a c a a t a t c t c t g c a a t g c g g c a a t t c a g t g g t t c g t c c a a t c c a t g t c a g a c c c g t c t t a t g t t a t a g a g a c g t t a c g c c g t t a a g t c a c c a a g c a g g t t a g g t a c a g t c t g g g c a g a

5280 RSV promoter g t t g c c t t c c t a a t a a g g c a c g a t c g t a c c a c c t t a c t t c c a c c a a t c g g c a t g c a c g g t c a a c g g a a g g a t t a t t c c g t g c t a g c a t g g t g g a a t g a a g g t g g t t a g c c g t a c g t g c c a

5340 RSV promoter g c t t t t t c t c t c c t t g t a a g g c a t g t t g c t a a c t c a t c g t t a c c a t g t t g c a a g a c t a c a c g a a a a a g a g a g g a a c a t t c c g t a c a a c g a t t g a g t a g c a a t g g t a c a a c g t t c t g a t g t

5400 RSV promoter T3 promoter G G A A A T C A C T C C T3 a g a g t a t t g c a t a a g a c t a c a t t a a g c t t g c a g c t c c a g c t t t t g t t c c c t t t a g t g a g g t c t c a t a a c g t a t t c t g a t g t a a t t c g a a c g t c g a g g t c g a a a a c a a g g g a a a t c a c t c c

5460 T3 promoter M13 rev lac operator C A A T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G M13/pUC Reverse g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g

5520 -10 -35 lac operator lac promoter C G A M13/pUC Reverse g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t

5580 CAP binding site a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t

5640 c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a

5700 G A G C G A G T G A C T G A G C G A L4440 t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c

5760 a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g

PciI 5820 a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a

5880 ori g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c

5940 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g

6000 ori c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g

6060 ori t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a

6120 ori c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a

6180 ori a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g

6240 ori a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t

6300 ori g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t

6360 ori g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c

6420 ori t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t

6480 a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c

6540 g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c

6600 285 *WHK AmpR *WHK a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a

6660 280 275 270 265 ILSAGIEAIQRNREDMTAQS AmpR ILSAGIEAIQRNREDMTAQS a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a

6720 260 255 250 245 GTTYIVVIRSPKGDPGLAAI AmpR GTTYIVVIRSPKGDPGLAAI c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a

6780 240 235 230 225 IGRSGREGAGSKDAIFWGAP AmpR IGRSGREGAGSKDAIFWGAP g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c

6840 220 215 210 205 LASRLLPGAVKDAEMWDILQ AmpR LASRLLPGAVKDAEMWDILQ a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c

6900 200 195 190 185 QRSALTLLEGTLLKRLTTAM AmpR QRSALTLLEGTLLKRLTTAM t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a

6960 180 175 170 165 AVPMTTDREDNPIAENLEPE AmpR AVPMTTDREDNPIAENLEPE t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g

7020 160 155 150 145 WRDLRTVHDGMNHLFATLEK AmpR WRDLRTVHDGMNHLFATLEK c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a

7080 140 135 130 125 PGGITTLLLNAATNDSMTIA AmpR PGGITTLLLNAATNDSMTIA c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g

7140 120 115 110 105 ASCLERVTMGDTLHKETVPS AmpR ASCLERVTMGDTLHKETVPS a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t

7200 100 95 90 85 YEVLDNQSYHIRRGLQEQGA AmpR YEVLDNQSYHIRRGLQEQGA g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g

7260 80 75 70 65 DIRSLVAGCLLVKFTSMMPF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R DIRSLVAGCLLVKFTSMMPF g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t

7320 60 55 50 45 REEPRFSELIKGSNLDLEIY AmpR REEPRFSELIKGSNLDLEIY a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t

7380 40 35 30 25 GVRAGLQDEADKVKVLTEPH AmpR GVRAGLQDEADKVKVLTEPH a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c

7440 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFHQ AmpR AFVPLCFAAFFPILAVRFHQ a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c

7500 1 signal sequence ISM AmpR AmpR promoter ISM a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a

7560 AmpR promoter g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a

7620 f1 ori t c c c c g a a a a g t g c c a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t a g g g g c t t t t c a c g g t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a

7680 f1 ori t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t

7740 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t

7800 f1 ori t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t

DraIII 7860 f1 ori c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a

7920 f1 ori c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c

7980 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g

8040 f1 ori a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c c a t t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g g t a

8100 t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t

8160 c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a

8220 M13 fwd T7 promoter C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C A C T A T A G T7 t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g a c t c a c t a t a g a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c t g a g t g a t a t c

8280 T7 promoter SV40 ori G G T7 g g c g a a t t g g g t a c g t c c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c t c c g c t t a a c c c a t g c a g g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c g a

SfiI 8340 SV40 ori c a g a g g c c g a g g c g g c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g t c t c c g g c t c c g c c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c

8400 SV40 ori g a g a a t g g g c g g a a c t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a t a g c t c t t a c c c g c c t t g a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t a t c

8460 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev c t a g a g c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g g a t c t c g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c

8520 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t

8580 SV40 poly(A) signal a g c t g c a a t a a a c a a g t t c c t c t c a c t c t c t g a t a t t c a t t t c t t t g c a a g t t a t a a a t a t c g a c g t t a t t t g t t c a a g g a g a g t g a g a g a c t a t a a g t a a a g a a a c g t t c a a t a t t t a t

8640 3' LTR ( Δ U3) c t g a a t a a t a a g a t g a c a t g a a c t a c t a c t g c t a g a g a t t t t c c a c a c t g a c t a a a a g g g g a c t t a t t a t t c t a c t g t a c t t g a t g a t g a c g a t c t c t a a a a g g t g t g a c t g a t t t t c c c

8700 3' LTR ( Δ U3) t c t g a g g g a t c t c t a g t t a c c a g a g t c a c a c a a c a g a c g g g c a c a c a c t a c t t g a a g c a c a g a c t c c c t a g a g a t c a a t g g t c t c a g t g t g t t g t c t g c c c g t g t g t g a t g a a c t t c g t g

8760 3' LTR ( Δ U3) t c a a g g c a a g c t t t a t t g a g g c t t a a g c a g t g g g t t c c c t a g t t a g c c a g a g a g c t c c c a a g t t c c g t t c g a a a t a a c t c c g a a t t c g t c a c c c a a g g g a t c a a t c g g t c t c t c g a g g g t

8820 3' LTR ( Δ U3) g g c t c a g a t c t g g t c t a a c c a g a g a g a c c c a g t a c a a g c a a a a a g c a g a t c t t g t c t t c g c c g a g t c t a g a c c a g a t t g g t c t c t c t g g g t c a t g t t c g t t t t t c g t c t a g a a c a g a a g c

BmtINheI 8880 3' LTR ( Δ U3) t t g g g a g t g a g c t a g c c c t t c c a g t c c c c c c t t t t c t t t t a a a a a g t g g c t a a g a t c t a c a a c c c t c a c t c g a t c g g g a a g g t c a g g g g g g a a a a g a a a a t t t t t c a c c g a t t c t a g a t g

Acc65IKpnI 8940 130 *GEW BSD a g c t g c c t t g t a a g t c a t t g g t c t t a a a g g t a c c g a g c t c g a a t t g t g c t t a g c c c t c c c t c g a c g g a a c a t t c a g t a a c c a g a a t t t c c a t g g c t c g a g c t t a a c a c g a a t c g g g a g g g

9000 125 120 115 110 VYGSPLLERIGVATPQGDSD BSD a c a c a t a a c c a g a g g g c a g c a a t t c a c g a a t c c c a a c t g c c g t c g g c t g t c c a t c a c t g t t g t g t a t t g g t c t c c c g t c g t t a a g t g c t t a g g g t t g a c g g c a g c c g a c a g g t a g t g a c a

BsaBI* 9060 105 100 95 90 KVIAKIGPHLDLLVQRCRGC BSD c c t t c a c t a t g g c t t t g a t c c c a g g a t g c a g a t c g a g a a g c a c c t g t c g g c a c c g t c c g c g g a a g t g a t a c c g a a a c t a g g g t c c t a c g t c t a g c t c t t c g t g g a c a g c c g t g g c a g g c g

9120 85 80 75 70 PSLIGRNENGIAVICTLNGA BSD a g g g g c t c a a g a t g c c c c t g t t c t c a t t t c c g a t c g c g a c g a t a c a a g t c a g g t t g c c a g t c c c c g a g t t c t a c g g g g a c a a g a g t a a a g g c t a g c g c t g c t a t g t t c a g t c c a a c g g t c

9180 65 60 55 50 AAAAATGLVVLEACPGGTFH BSD c t g c c g c a g c a g c a g c a g t g c c c a g c a c c a c g a g t t c t g c a c a a g g t c c c c c a g t a a a a t g a c g g c g t c g t c g t c g t c a c g g g t c g t g g t g c t c a a g a c g t g t t c c a g g g g g t c a t t t t a

9240 45 40 35 30 YVNVGTFIRGDSSLAASAVS BSD g a t a t a c a t t g a c a c c a g t g a a g a t g c g g c c g t c g c t a g a g a g a g c t g c g c t g g c g a c g c c t a t a t g t a a c t g t g g t c a c t t c t a c g c c g g c a g c g a t c t c t c t c g a c g c g a c c g c t g c g

9300 25 20 15 10 YDESIPISNITATAREILTS BSD t g t a g t c t t c a g a g a t g g g g a t g c t g t t g a t t g t a g c c g t t g c t c t t t c a a t g a g g g t g g a c a t c a g a a g t c t c t a c c c c t a c g a c a a c t a a c a t c g g c a a c g a g a a a g t t a c t c c c a c c

9360 5 1 EEQSLPKAM BSD EM7 promoter a t t c t t c t t g a g a c a a a g g c t t g g c c a t g g t t t a g t t c c t c a c c t t g t c g t a t t a t a c t a t a a g a a g a a c t c t g t t t c c g a a c c g g t a c c a a a t c a a g g a g t g g a a c a g c a t a a t a t g a t

PmlI 9420 EM7 promoter t g c c g a t a t a c t a t g c c g a t g a t t a a t t g t c a a c a c g t g c t g a t c a g a t c c g a a a a t g g a a c g g c t a t a t g a t a c g g c t a c t a a t t a a c a g t t g t g c a c g a c t a g t c t a g g c t t t t a c c t

TspMIXmaISmaI 3ʹ 5ʹ 9450 SV40 promoter t a t a c a a g c t c c c g g g a g c t t t t t g c a a a a a t a t g t t c g a g g g c c c t c g a a a a a c g t t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.