Addgene pHAGE-EF1aL-E690Kmut_ABCA3-GFP Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pHAGE-EF1aL-E690Kmut_ABCA3-GFP Sequencing Result


Map View

M13 rev M13 Reverse (12,641 .. 12,657) lac operator M13/pUC Reverse (12,622 .. 12,644) CAP binding site AvrII (12,467) L4440 (12,286 .. 12,303) DrdI (12,086) pBR322ori-F (12,033 .. 12,052) PvuI (10,931) Amp-R (10,731 .. 10,750) SspI (10,495) pBRforEco (10,322 .. 10,340) PacI (10,285) PsiI (10,238) WPRE-R (9360 .. 9380) BsmBI - Esp3I (7255) SbfI (6710) EBV-rev (12,707 .. 12,726) NruI * (833) KflI (1934) EcoRI (2028) SpeI (2212) EF-1α promoter PspOMI (2836) ApaI (2840) PaeR7I - XhoI (3188) EF1a-F (3349 .. 3369) BamHI (3566) BclI * (3761) SphI (4105) AfeI (5823) AscI (6045) PshAI (6262) pHAGE-EF1aL-E690Kmut_ABCA3-GFP 12,917 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t

120 3' LTR c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g

180 3' LTR t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t

240 3' LTR a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c

300 3' LTR a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c

360 3' LTR a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c

420 3' LTR c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a

480 3' LTR c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

540 3' LTR g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

600 3' LTR t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

660 3' LTR a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

720 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

780 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

NruI* 840 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

900 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

960 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1020 *I g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1080 MIYYLLLGRNHADFSLSLLC c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1140 WPLKLCSLPLAFCFYSWRVA c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

1200 MRDNWRS LPRGSIKLGPPPSILSLQLL a a g c g g c c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c

1260 ELYKYKVVKIEPLGVAPTKA SNYLYLTTFISGNPTAGVLA t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g

1320 RRE KRRVVQREKRAVGIGALFLG FLLTTCLSFLATPIPAKNRP a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c

1380 RRE FLGAAGSTMGAASMTLTVQA NKPAAPLVIPAADIVSVTCA g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g

1440 RRE RQLLSGIVQQQNNLLRAIEA LCNNDPITCCCFLKSLAISA c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g

1500 RRE QQHLLQLTVWGIKQLQARIL CCCRNCSVTQPM g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a

1560 RRE AVERYLKDQQLLGIWGCSGK g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t

1620 LICTTAVPWNASWSNKSLEQ a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t

1680 IWNHTTWMEWDREINNYTSL g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a

1740 1 5 KNEQELL gp41 peptide IHSLIEESQNQQEKNEQELL a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a

1800 10 15 ELDKWASL (in frame with gp41 peptide) WNWFNITNWLWY gp41 peptide ELDKWASLWNWFNITNWLWY g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t

1860 (in frame with gp41 peptide) IKLFIMIVGGLVGLRIVFAV IKLFIMIVGGLVGLRIVFAV t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a

1920 (in frame with gp41 peptide) LSIVNRVRQGYSPLSFQTHL LSIVNRVRQGYSPLSFQTHL a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a

KflI 1980 (in frame with gp41 peptide) PTPRGPDRPEGIEEEGGERD PTPRGPDRPEGIEEEGGERD c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t

EcoRI 2040 (in frame with gp41 peptide) RDRSIRLVNGSRRYRRIHKW RDRSIRLVNGSRRYRRIHKW c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g c c g a a t t c a c a a a t g g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c g g c t t a a g t g t t t a c

2100 (in frame with gp41 peptide) QYSSTILKEKGGLGGTVQGK cPPT/CTS QYSSTILKEKGGLGGTVQGK g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t

2160 E* cPPT/CTS E* a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t

SpeI 2220 cPPT/CTS a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a g t t t g g a c t a g t c g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t c a a a c c t g a t c a g c a

2280 EF-1 α promoter g a g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g c t c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c

2340 EF-1 α promoter g g g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a c c c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t

2400 EF-1 α promoter g t g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c

2460 EF-1 α promoter EF-1 α intron A c a g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g g t c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c

2520 EF-1 α promoter EF-1 α intron A c c g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a g g c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t

2580 EF-1 α promoter EF-1 α intron A *KWRAATRSEQDRAEPQFHT t t a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t a a t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a

2640 EF-1 α promoter EF-1 α intron A PSNSAKRKLLGKAEHKLQPR g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a

2700 EF-1 α promoter EF-1 α intron A AQASPGGRAFRTAGERRDRQ g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c

2760 EF-1 α promoter EF-1 α intron A KRYTELWKFNKIVQQSAKKR c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a

2820 EF-1 α promoter EF-1 α intron A ALYDQLHPGLDACQYKPKQP g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c

PspOMIApaI 2880 EF-1 α promoter EF-1 α intron A RPRRRPGHTGACMNPSAPGA c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g

2940 EF-1 α promoter EF-1 α intron A LAAVSFRVPTTELQGAQEPA g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g

3000 EF-1 α promoter EF-1 α intron A QGRAATYRGARPPLAPGTPV c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g

3060 EF-1 α promoter EF-1 α intron A LQTLPFIAAERGQQLSSLIS c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t

3120 EF-1 α promoter EF-1 α intron A SAASPLAPPHTVWVFSFPRE g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g

3180 EF-1 α promoter EF-1 α intron A TRLRRKM c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c

PaeR7IXhoI 3240 EF-1 α promoter EF-1 α intron A a t t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t a a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t

3300 EF-1 α promoter EF-1 α intron A t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a

3360 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A EF1a-F a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t

3420 EF-1 α promoter EF-1 α intron A C A G T G G T T C EF1a-F c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a a g c g g c c a a t t c g c c c t t t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t t c g c c g g t t a a g c g g g a a a

3480 MAVLRQLA t c t c t a g c a g c a c t g g g c g t c c c t t t c a g c a a g a c g a t g g c t g t g c t c a g g c a g c t g g c g a g a g a t c g t c g t g a c c c g c a g g g a a a g t c g t t c t g c t a c c g a c a c g a g t c c g t c g a c c g c

3540 LLLWKNYTLQKRKVLVTVLE c t c c t c c t c t g g a a g a a c t a c a c c c t g c a g a a g c g g a a g g t c c t g g t g a c g g t c c t g g a a g a g g a g g a g a c c t t c t t g a t g t g g g a c g t c t t c g c c t t c c a g g a c c a c t g c c a g g a c c t t

BamHI 3600 LFLPLLFSGILIWLRLKIQS c t c t t c c t g c c a t t g c t g t t t t c t g g g a t c c t c a t c t g g c t c c g c t t g a a g a t t c a g t c g g a g a a g g a c g g t a a c g a c a a a a g a c c c t a g g a g t a g a c c g a g g c g a a c t t c t a a g t c a g c

3660 ENVPNATIYPGQSIQELPLF g a a a a t g t g c c c a a c g c c a c c a t c t a c c c g g g c c a g t c c a t c c a g g a g c t g c c t c t g t t c c t t t t a c a c g g g t t g c g g t g g t a g a t g g g c c c g g t c a g g t a g g t c c t c g a c g g a g a c a a g

3720 FTFPPPGDTWELAYIPSHSD t t c a c c t t c c c t c c g c c a g g a g a c a c c t g g g a g c t t g c c t a c a t c c c t t c t c a c a g t g a c a a g t g g a a g g g a g g c g g t c c t c t g t g g a c c c t c g a a c g g a t g t a g g g a a g a g t g t c a c t g

BclI* 3780 AAKTVTETVRRALVINMRVR g c t g c c a a g a c c g t c a c t g a g a c a g t g c g c a g g g c a c t t g t g a t c a a c a t g c g a g t g c g c c g a c g g t t c t g g c a g t g a c t c t g t c a c g c g t c c c g t g a a c a c t a g t t g t a c g c t c a c g c g

3840 GFPSEKDFEDYIRYDNCSSS g g c t t t c c c t c c g a g a a g g a c t t t g a g g a c t a c a t t a g g t a c g a c a a c t g c t c g t c c a g c c c g a a a g g g a g g c t c t t c c t g a a a c t c c t g a t g t a a t c c a t g c t g t t g a c g a g c a g g t c g

3900 VLAAVVFEHPFNHSKEPLPL g t g c t g g c c g c c g t g g t c t t c g a g c a c c c c t t c a a c c a c a g c a a g g a g c c c c t g c c g c t g c a c g a c c g g c g g c a c c a g a a g c t c g t g g g g a a g t t g g t g t c g t t c c t c g g g g a c g g c g a c

3960 AVKYHLRFSYTRRNYMWTQT g c g g t g a a a t a t c a c c t a c g g t t c a g t t a c a c a c g g a g a a a t t a c a t g t g g a c c c a a a c a c g c c a c t t t a t a g t g g a t g c c a a g t c a a t g t g t g c c t c t t t a a t g t a c a c c t g g g t t t g t

4020 GSFFLKETEGWHTTSLFPLF g g c t c c t t t t t c c t g a a a g a g a c a g a a g g c t g g c a c a c t a c t t c c c t t t t c c c g c t t t t c c c g a g g a a a a a g g a c t t t c t c t g t c t t c c g a c c g t g t g a t g a a g g g a a a a g g g c g a a a a g

4080 PNPGPREPTSPDGGEPGYIR c c a a a c c c a g g a c c a a g g g a a c c t a c a t c c c c t g a t g g c g g a g a a c c t g g g t a c a t c c g g g g t t t g g g t c c t g g t t c c c t t g g a t g t a g g g g a c t a c c g c c t c t t g g a c c c a t g t a g g c c

SphI 4140 EGFLAVQHAVDRAIMEYHAD g a a g g c t t c c t g g c c g t g c a g c a t g c t g t g g a c c g g g c c a t c a t g g a g t a c c a t g c t g a t c t t c c g a a g g a c c g g c a c g t c g t a c g a c a c c t g g c c c g g t a g t a c c t c a t g g t a c g a c t a

4200 AATRQLFQRLTVTIKRFPYP g c c g c c a c a c g c c a g c t g t t c c a g a g a c t g a c g g t g a c c a t c a a g a g g t t c c c g t a c c c g c g g c g g t g t g c g g t c g a c a a g g t c t c t g a c t g c c a c t g g t a g t t c t c c a a g g g c a t g g g c

4260 PFIADPFLVAIQYQLPLLLL c c g t t c a t c g c a g a c c c c t t c c t c g t g g c c a t c c a g t a c c a g c t g c c c c t g c t g c t g c t g g g c a a g t a g c g t c t g g g g a a g g a g c a c c g g t a g g t c a t g g t c g a c g g g g a c g a c g a c g a c

4320 LSFTYTALTIARAVVQEKER c t c a g c t t c a c c t a c a c c g c g c t c a c c a t t g c c c g t g c t g t c g t g c a g g a g a a g g a a a g g g a g t c g a a g t g g a t g t g g c g c g a g t g g t a a c g g g c a c g a c a g c a c g t c c t c t t c c t t t c c

4380 RLKEYMRMMGLSSWLHWSAW a g g c t g a a g g a g t a c a t g c g c a t g a t g g g g c t c a g c a g c t g g c t g c a c t g g a g t g c c t g g t c c g a c t t c c t c a t g t a c g c g t a c t a c c c c g a g t c g t c g a c c g a c g t g a c c t c a c g g a c c

4440 FLLFFLFLLIAASFMTLLFC t t c c t c t t g t t c t t c c t c t t c c t c c t c a t c g c c g c c t c c t t c a t g a c c c t g c t c t t c t g t a a g g a g a a c a a g a a g g a g a a g g a g g a g t a g c g g c g g a g g a a g t a c t g g g a c g a g a a g a c a

4500 VKVKPNVAVLSRSDPSLVLA g t c a a g g t g a a g c c a a a t g t a g c c g t g c t g t c c c g c a g c g a c c c c t c c c t g g t g c t c g c c c a g t t c c a c t t c g g t t t a c a t c g g c a c g a c a g g g c g t c g c t g g g g a g g g a c c a c g a g c g g

4560 FLLCFAISTISFSFMVSTFF t t c c t g c t g t g c t t c g c c a t c t c t a c c a t c t c c t t c a g c t t c a t g g t c a g c a c c t t c t t c a a g g a c g a c a c g a a g c g g t a g a g a t g g t a g a g g a a g t c g a a g t a c c a g t c g t g g a a g a a g

4620 SKANMAAAFGGFLYFFTYIP a g c a a a g c c a a c a t g g c a g c a g c c t t c g g a g g c t t c c t c t a c t t c t t c a c c t a c a t c c c c t c g t t t c g g t t g t a c c g t c g t c g g a a g c c t c c g a a g g a g a t g a a g a a g t g g a t g t a g g g g

4680 YFFVAPRYNWMTLSQKLCSC t a c t t c t t c g t g g c c c c t c g g t a c a a c t g g a t g a c t c t g a g c c a g a a g c t c t g c t c c t g c a t g a a g a a g c a c c g g g g a g c c a t g t t g a c c t a c t g a g a c t c g g t c t t c g a g a c g a g g a c g

4740 LLSNVAMAMGAQLIGKFEAK c t c c t g t c t a a t g t c g c c a t g g c a a t g g g a g c c c a g c t c a t t g g g a a a t t t g a g g c g a a a g a g g a c a g a t t a c a g c g g t a c c g t t a c c c t c g g g t c g a g t a a c c c t t t a a a c t c c g c t t t

4800 GMGIQWRDLLSPVNVDDDFC g g c a t g g g c a t c c a g t g g c g a g a c c t c c t g a g t c c c g t c a a c g t g g a c g a c g a c t t c t g c c c g t a c c c g t a g g t c a c c g c t c t g g a g g a c t c a g g g c a g t t g c a c c t g c t g c t g a a g a c g

4860 FGQVLGMLLLDSVLYGLVTW t t c g g g c a g g t g c t g g g g a t g c t g c t g c t g g a c t c t g t g c t c t a t g g c c t g g t g a c c t g g a a g c c c g t c c a c g a c c c c t a c g a c g a c g a c c t g a g a c a c g a g a t a c c g g a c c a c t g g a c c

4920 YMEAVFPGQFGVPQPWYFFI t a c a t g g a g g c c g t c t t c c c a g g g c a g t t c g g c g t g c c t c a g c c c t g g t a c t t c t t c a t c a t g t a c c t c c g g c a g a a g g g t c c c g t c a a g c c g c a c g g a g t c g g g a c c a t g a a g a a g t a g

4980 MPSYWCGKPRAVAGKEEEDS a t g c c c t c c t a t t g g t g t g g g a a g c c a a g g g c g g t t g c a g g g a a g g a g g a a g a a g a c a g t t a c g g g a g g a t a a c c a c a c c c t t c g g t t c c c g c c a a c g t c c c t t c c t c c t t c t t c t g t c a

5040 DPEKALRNEYFEAEPEDLVA g a c c c c g a g a a a g c a c t c a g a a a c g a g t a c t t t g a a g c c g a g c c a g a g g a c c t g g t g g c g c t g g g g c t c t t t c g t g a g t c t t t g c t c a t g a a a c t t c g g c t c g g t c t c c t g g a c c a c c g c

5100 GIKIKHLSKVFRVGNKDRAA g g g a t c a a g a t c a a g c a c c t g t c c a a g g t g t t c a g g g t g g g a a a t a a g g a c a g g g c g g c c c c c t a g t t c t a g t t c g t g g a c a g g t t c c a c a a g t c c c a c c c t t t a t t c c t g t c c c g c c g g

5160 VRDLNLNLYEGQITVLLGHN g t c a g a g a c c t g a a c c t c a a c c t g t a c g a g g g a c a g a t c a c c g t c c t g c t g g g c c a c a a c c a g t c t c t g g a c t t g g a g t t g g a c a t g c t c c c t g t c t a g t g g c a g g a c g a c c c g g t g t t g

5220 GAGKTTTLSMLTGLFPPTSG g g t g c c g g g a a g a c c a c c a c c c t c t c c a t g c t c a c a g g t c t c t t t c c c c c g a c c a g t g g a c c a c g g c c c t t c t g g t g g t g g g a g a g g t a c g a g t g t c c a g a g a a a g g g g g c t g g t c a c c t

5280 RAYISGYEISQDMVQIRKSL c g g g c a t a c a t c a g c g g g t a t g a a a t t t c c c a g g a c a t g g t t c a g a t c c g g a a g a g c c t g g c c c g t a t g t a g t c g c c c a t a c t t t a a a g g g t c c t g t a c c a a g t c t a g g c c t t c t c g g a c

5340 GLCPQHDILFDNLTVAEHLY g g c c t g t g c c c g c a g c a c g a c a t c c t g t t t g a c a a c t t g a c a g t c g c a g a g c a c c t t t a t c c g g a c a c g g g c g t c g t g c t g t a g g a c a a a c t g t t g a a c t g t c a g c g t c t c g t g g a a a t a

5400 FYAQLKGLSRQKCPEEVKQM t t c t a c g c c c a g c t g a a g g g c c t g t c a c g t c a g a a g t g c c c t g a a g a a g t c a a g c a g a t g a a g a t g c g g g t c g a c t t c c c g g a c a g t g c a g t c t t c a c g g g a c t t c t t c a g t t c g t c t a c

5460 LHIIGLEDKWNSRSRFLSGG c t g c a c a t c a t c g g c c t g g a g g a c a a g t g g a a c t c a c g g a g c c g c t t c c t g a g c g g g g g c g a c g t g t a g t a g c c g g a c c t c c t g t t c a c c t t g a g t g c c t c g g c g a a g g a c t c g c c c c c g

5520 MRRKLSIGIALIAGSKVLIL a t g a g g c g c a a g c t c t c c a t c g g c a t c g c c c t c a t c g c a g g c t c c a a g g t g c t g a t a c t g t a c t c c g c g t t c g a g a g g t a g c c g t a g c g g g a g t a g c g t c c g a g g t t c c a c g a c t a t g a c

5580 DKPTSGMDAISRRAIWDLLQ g a c a a g c c c a c c t c g g g c a t g g a c g c c a t c t c c a g g a g g g c c a t c t g g g a t c t t c t t c a g c t g t t c g g g t g g a g c c c g t a c c t g c g g t a g a g g t c c t c c c g g t a g a c c c t a g a a g a a g t c

5640 RQKSDRTIVLTTHFMDEADL c g g c a g a a a a g t g a c c g c a c c a t c g t g c t g a c c a c c c a c t t c a t g g a c g a g g c t g a c c t g g c c g t c t t t t c a c t g g c g t g g t a g c a c g a c t g g t g g g t g a a g t a c c t g c t c c g a c t g g a c

5700 LGDRIAIMAKGELQCCGSSL c t g g g a g a c c g c a t c g c c a t c a t g g c c a a g g g g g a g c t g c a g t g c t g c g g g t c c t c g c t g g a c c c t c t g g c g t a g c g g t a g t a c c g g t t c c c c c t c g a c g t c a c g a c g c c c a g g a g c g a c

5760 FLKQKYGAGYHMTLVKEPHC *MVSTFSGCQ t t c c t c a a g c a g a a a t a c g g t g c c g g c t a t c a c a t g a c g c t g g t g a a g g a g c c g c a c t g c a a g g a g t t c g t c t t t a t g c c a c g g c c g a t a g t g t a c t g c g a c c a c t t c c t c g g c g t g a c g

5820 NPEDISQLVHHHVPNATLES LGSSMEWSTWWWTGLAVSSL a a c c c g g a a g a c a t c t c c c a g c t g g t c c a c c a c c a c g t g c c c a a c g c c a c g c t g g a g a g c t t g g g c c t t c t g t a g a g g g t c g a c c a g g t g g t g g t g c a c g g g t t g c g g t g c g a c c t c t c g

AfeI 5880 SAGAELSFILPRESTHRFEG LAPASSDKMRGLSLVCLNSP a g c g c t g g g g c c g a g c t g t c t t t c a t c c t t c c c a g a g a g a g c a c g c a c a g g t t t g a a g g t t c g c g a c c c c g g c t c g a c a g a a a g t a g g a a g g g t c t c t c t c g t g c g t g t c c a a a c t t c c a

5940 LFAKLEKKQKELGIASFGAS RKALSSFFCFSSPMALKPAD c t c t t t g c t a a a c t g g a g a a g a a g c a g a a a g a g c t g g g c a t t g c c a g c t t t g g g g c a t c c g a g a a a c g a t t t g a c c t c t t c t t c g t c t t t c t c g a c c c g t a a c g g t c g a a a c c c c g t a g g

6000 ITTMEEVFLRVGKLVDSSMD MVVMSSTKRRTPFSTSLLIS a t c a c c a c c a t g g a g g a a g t c t t c c t t c g g g t c g g g a a g c t g g t g g a c a g c a g t a t g g a c t a g t g g t g g t a c c t c c t t c a g a a g g a a g c c c a g c c c t t c g a c c a c c t g t c g t c a t a c c t g

AscI 6060 IQAIQLPALQYQHERRASDW MWAMWSGARCYWCSLRALSQ a t c c a g g c c a t c c a g c t c c c t g c c c t g c a g t a c c a g c a c g a g a g g c g c g c c a g c g a c t g g t a g g t c c g g t a g g t c g a g g g a c g g g a c g t c a t g g t c g t g c t c t c c g c g c g g t c g c t g a c c

6120 AVDSNLCGAMDPSDGIGALI ATSLLRQPAMSGESPMPARM g c t g t g g a c a g c a a c c t c t g t g g g g c c a t g g a c c c c t c c g a c g g c a t t g g a g c c c t c a t c c g a c a c c t g t c g t t g g a g a c a c c c c g g t a c c t g g g g a g g c t g c c g t a a c c t c g g g a g t a g

6180 EEERTAVKLNTGLALHCQQF SSSRVATLSLVPSARCQWCN g a g g a g g a g c g c a c c g c t g t c a a g c t c a a c a c t g g g c t c g c c c t g c a c t g c c a g c a a t t c c t c c t c c t c g c g t g g c g a c a g t t c g a g t t g t g a c c c g a g c g g g a c g t g a c g g t c g t t a a g

6240 WAMFLKKAAYSWREWKMVAA QAMNRFFAAYLQRSHFITAA t g g g c c a t g t t c c t g a a g a a g g c c g c a t a c a g c t g g c g c g a g t g g a a a a t g g t g g c g g c a a c c c g g t a c a a g g a c t t c t t c c g g c g t a t g t c g a c c g c g c t c a c c t t t t a c c a c c g c c g t

PshAI 6300 QVLVPLTCVTLALLAINYSS CTRTGRVQTVRARRAM c a g g t c c t g g t g c c t c t g a c c t g c g t c a c c c t g g c c c t c c t g g c c a t c a a c t a c t c c t c g g t c c a g g a c c a c g g a g a c t g g a c g c a g t g g g a c c g g g a g g a c c g g t a g t t g a t g a g g a g c

6360 ELFDDPMLRLTLGEYGRTVV g a g c t c t t c g a c g a c c c c a t g c t g a g g c t g a c c t t g g g c g a g t a c g g c a g a a c c g t c g t g c t c g a g a a g c t g c t g g g g t a c g a c t c c g a c t g g a a c c c g c t c a t g c c g t c t t g g c a g c a c

6420 PFSVPGTSQLGQQLSEHLKD c c c t t c t c a g t t c c c g g g a c c t c c c a g c t g g g t c a g c a g c t g t c a g a g c a t c t g a a a g a c g g g a a g a g t c a a g g g c c c t g g a g g g t c g a c c c a g t c g t c g a c a g t c t c g t a g a c t t t c t g

6480 ALQAEGQEPREVLGDLEEFL g c a c t g c a g g c t g a g g g a c a g g a g c c c c g c g a g g t g c t c g g t g a c c t g g a g g a g t t c t t g c g t g a c g t c c g a c t c c c t g t c c t c g g g g c g c t c c a c g a g c c a c t g g a c c t c c t c a a g a a c

6540 IFRASVEGGGFNERCLVAAS a t c t t c a g g g c t t c t g t g g a g g g g g g c g g c t t t a a t g a g c g g t g c c t t g t g g c a g c g t c c t a g a a g t c c c g a a g a c a c c t c c c c c c g c c g a a a t t a c t c g c c a c g g a a c a c c g t c g c a g g

6600 FRDVGERTVVNALFNNQAYH t t c a g a g a t g t g g g a g a g c g c a c g g t c g t c a a c g c c t t g t t c a a c a a c c a g g c g t a c c a c a a g t c t c t a c a c c c t c t c g c g t g c c a g c a g t t g c g g a a c a a g t t g t t g g t c c g c a t g g t g

6660 SPATALAVVDNLLFKLLCGP t c t c c a g c c a c t g c c c t g g c c g t c g t g g a c a a c c t t c t g t t c a a g c t g c t g t g c g g g c c t a g a g g t c g g t g a c g g g a c c g g c a g c a c c t g t t g g a a g a c a a g t t c g a c g a c a c g c c c g g a

SbfI 6720 HASIVVSNFPQPRSALQAAK c a c g c c t c c a t t g t g g t c t c c a a c t t c c c c c a g c c c c g g a g c g c c c t g c a g g c t g c c a a g g t g c g g a g g t a a c a c c a g a g g t t g a a g g g g g t c g g g g c c t c g c g g g a c g t c c g a c g g t t c

6780 DQFNEGRKGFDIALNLLFAM g a c c a g t t t a a c g a g g g c c g g a a g g g a t t c g a c a t t g c c c t c a a c c t g c t c t t c g c c a t g c t g g t c a a a t t g c t c c c g g c c t t c c c t a a g c t g t a a c g g g a g t t g g a c g a g a a g c g g t a c

6840 AFLASTFSILAVSERAVQAK g c a t t c t t g g c c a g c a c g t t c t c c a t c c t g g c g g t c a g c g a g a g g g c c g t g c a g g c c a a g c g t a a g a a c c g g t c g t g c a a g a g g t a g g a c c g c c a g t c g c t c t c c c g g c a c g t c c g g t t c

6900 HVQFVSGVHVASFWLSALLW c a t g t g c a g t t t g t g a g t g g a g t c c a c g t g g c c a g t t t c t g g c t c t c t g c t c t g c t g t g g g t a c a c g t c a a a c a c t c a c c t c a g g t g c a c c g g t c a a a g a c c g a g a g a c g a g a c g a c a c c

6960 DLISFLIPSLLLLVVFKAFD g a c c t c a t c t c c t t c c t c a t c c c c a g t c t g c t g c t g c t g g t g g t g t t t a a g g c c t t c g a c c t g g a g t a g a g g a a g g a g t a g g g g t c a g a c g a c g a c g a c c a c c a c a a a t t c c g g a a g c t g

7020 VRAFTRDGHMADTLLLLLLY g t g c g t g c c t t c a c g c g g g a c g g c c a c a t g g c t g a c a c c c t g c t g c t g c t c c t g c t c t a c c a c g c a c g g a a g t g c g c c c t g c c g g t g t a c c g a c t g t g g g a c g a c g a c g a g g a c g a g a t g

7080 GWAIIPLMYLMNFFFLGAAT g g c t g g g c c a t c a t c c c c c t c a t g t a c c t g a t g a a c t t c t t c t t c t t g g g g g c g g c c a c t c c g a c c c g g t a g t a g g g g g a g t a c a t g g a c t a c t t g a a g a a g a a g a a c c c c c g c c g g t g a

7140 AYTRLTIFNILSGIATFLMV g c c t a c a c g a g g c t g a c c a t c t t c a a c a t c c t g t c a g g c a t c g c c a c c t t c c t g a t g g t c c g g a t g t g c t c c g a c t g g t a g a a g t t g t a g g a c a g t c c g t a g c g g t g g a a g g a c t a c c a g

7200 TIMRIPAVKLEELSKTLDHV a c c a t c a t g c g c a t c c c a g c t g t a a a a c t g g a a g a a c t t t c c a a a a c c c t g g a t c a c g t g t g g t a g t a c g c g t a g g g t c g a c a t t t t g a c c t t c t t g a a a g g t t t t g g g a c c t a g t g c a c

BsmBIEsp3I 7260 FLVLPNHCLGMAVSSFYENY t t c c t g g t g c t g c c c a a c c a c t g t c t g g g g a t g g c a g t c a g c a g t t t c t a c g a g a a c t a c a a g g a c c a c g a c g g g t t g g t g a c a g a c c c c t a c c g t c a g t c g t c a a a g a t g c t c t t g a t g

7320 ETRRYCTSSEVAAHYCKKYN g a g a c g c g g a g g t a c t g c a c c t c c t c c g a g g t c g c c g c c c a c t a c t g c a a g a a a t a t a a c c t c t g c g c c t c c a t g a c g t g g a g g a g g c t c c a g c g g c g g g t g a t g a c g t t c t t t a t a t t g

7380 IQYQENFYAWSAPGVGRFVA a t c c a g t a c c a g g a g a a c t t c t a t g c c t g g a g c g c c c c g g g g g t c g g c c g g t t t g t g g c c t a g g t c a t g g t c c t c t t g a a g a t a c g g a c c t c g c g g g g c c c c c a g c c g g c c a a a c a c c g g

7440 SMAASGCAYLILLFLIETNL t c c a t g g c c g c c t c a g g g t g c g c c t a c c t c a t c c t g c t c t t c c t c a t c g a g a c c a a c c t g a g g t a c c g g c g g a g t c c c a c g c g g a t g g a g t a g g a c g a g a a g g a g t a g c t c t g g t t g g a c

7500 LQRLRGILCALRRRRTLTEL * c t t c a g a g a c t c a g g g g c a t c c t c t g c g c c c t c c g g a g g a g g c g g a c a c t g a c a g a a t t a g a a g t c t c t g a g t c c c c g t a g g a g a c g c g g g a g g c c t c c t c c g c c t g t g a c t g t c t t a a t

7560 YTRMPVLPEDQDVADERTRI VGPHRHKRLVLIYRVLPGAD t a c a c c c g g a t g c c t g t g c t t c c t g a g g a c c a a g a t g t a g c g g a c g a g a g g a c c c g c a t c a t g t g g g c c t a c g g a c a c g a a g g a c t c c t g g t t c t a c a t c g c c t g c t c t c c t g g g c g t a g

7620 LAPSPDSLLHTPLIIKELSK QGGARVGQEVCRQNDLLEGL c t g g c c c c c a g c c c g g a c t c c c t g c t c c a c a c a c c t c t g a t t a t c a a g g a g c t c t c c a a g g a c c g g g g g t c g g g c c t g a g g g a c g a g g t g t g t g g a g a c t a a t a g t t c c t c g a g a g g t t c

7680 VYEQRVPLLAVDRLSLAVQK HVLLPHGEQGHVPEGERHLF g t g t a c g a g c a g c g g g t g c c c c t c c t g g c c g t g g a c a g g c t c t c c c t c g c g g t g c a g a a a c a c a t g c t c g t c g c c c a c g g g g a g g a c c g g c a c c t g t c c g a g a g g g a g c g c c a c g t c t t t

7740 GECFGLLGFNGAGKTTTFKM PLAEAQQAEISGPLGRSEFH g g g g a g t g c t t c g g c c t g c t g g g c t t c a a t g g a g c c g g g a a g a c c a c g a c t t t c a a a a t g c c c c t c a c g a a g c c g g a c g a c c c g a a g t t a c c t c g g c c c t t c t g g t g c t g a a a g t t t t a c

7800 LTGEESLTSGDAFVGGHRIS QGPLLAESRPIGKDPTVSDA c t g a c c g g g g a g g a g a g c c t c a c t t c t g g g g a t g c c t t t g t c g g g g g t c a c a g a a t c a g c g a c t g g c c c c t c c t c t c g g a g t g a a g a c c c c t a c g g a a a c a g c c c c c a g t g t c t t a g t c g

7860 SDVGKVRQRIGYCPQFDALL RIDSLHPLPDAVARLKIGQQ t c t g a t g t c g g a a a g g t g c g g c a g c g g a t c g g c t a c t g c c c g c a g t t t g a t g c c t t g c t g a g a c t a c a g c c t t t c c a c g c c g t c g c c t a g c c g a t g a c g g g c g t c a a a c t a c g g a a c g a c

7920 DHMTGREMLVMYARLRGIPE VVHCAPLHQDHVSPEPADRL g a c c a c a t g a c a g g c c g g g a g a t g c t g g t c a t g t a c g c t c g g c t c c g g g g c a t c c c t g a g c t g g t g t a c t g t c c g g c c c t c t a c g a c c a g t a c a t g c g a g c c g a g g c c c c g t a g g g a c t c

7980 RHIGACVENTLRGLLLEPHA AVDPGAHLVSQPAQQQLWMG c g c c a c a t c g g g g c c t g c g t g g a g a a c a c t c t g c g g g g c c t g c t g c t g g a g c c a c a t g c c g c g g t g t a g c c c c g g a c g c a c c t c t t g t g a g a c g c c c c g g a c g a c g a c c t c g g t g t a c g g

8040 NKLVRTYSGGNKRKLSTGIA VLQDPRVTTTVLPLQAGADG a a c a a g c t g g t c a g g a c g t a c a g t g g t g g t a a c a a g c g g a a g c t g a g c a c c g g c a t c g c c t t g t t c g a c c a g t c c t g c a t g t c a c c a c c a t t g t t c g c c t t c g a c t c g t g g c c g t a g c g g

8100 LIGEPAVIFLDEPSTGMDPV QDSLRSDDEQVLRGSAHVGH c t g a t c g g a g a g c c t g c t g t c a t c t t c c t g g a c g a g c c g t c c a c t g g c a t g g a c c c c g t g g a c t a g c c t c t c g g a c g a c a g t a g a a g g a c c t g c t c g g c a g g t g a c c g t a c c t g g g g c a c

8160 ARRLLWDTVARARESGKAII GPAQKPVGHCSGSLRALGDD g c c c g g c g c c t g c t t t g g g a c a c c g t g g c a c g a g c c c g a g a g t c t g g c a a g g c c a t c a t c c g g g c c g c g g a c g a a a c c c t g t g g c a c c g t g c t c g g g c t c t c a g a c c g t t c c g g t a g t a g

8220 ITSHSMEECEALCTRLAIMV DGGVAHLLTLGQAGPQGDHH a t c a c c t c c c a c a g c a t g g a g g a g t g t g a g g c c c t g t g c a c c c g g c t g g c c a t c a t g g t g t a g t g g a g g g t g t c g t a c c t c c t c a c a c t c c g g g a c a c g t g g g c c g a c c g g t a g t a c c a c

8280 QGQFKCLGSPQHLKSKFGSG LPLELAQAAGLVELALEAAA c a g g g g c a g t t c a a g t g c c t g g g c a g c c c c c a g c a c c t c a a g a g c a a g t t c g g c a g c g g c g t c c c c g t c a a g t t c a c g g a c c c g t c g g g g g t c g t g g a g t t c t c g t t c a a g c c g t c g c c g

8340 YSLRAKVQSEGQQEALEEFK VGQPGLHLTFPLLLRQLLEL t a c t c c c t g c g g g c c a a g g t g c a g a g t g a a g g g c a a c a g g a g g c g c t g g a g g a g t t c a a g a t g a g g g a c g c c c g g t t c c a c g t c t c a c t t c c c g t t g t c c t c c g c g a c c t c c t c a a g t t c

8400 AFVDLTFPGSVLEDEHQGMV GEHVQGKWAADQFILVLAHD g c c t t c g t g g a c c t g a c c t t t c c a g g c a g c g t c c t g g a a g a t g a g c a c c a a g g c a t g g t c c g g a a g c a c c t g g a c t g g a a a g g t c c g t c g c a g g a c c t t c t a c t c g t g g t t c c g t a c c a g

8460 HYHLPGRDLSWAKVFGILEK M c a t t a c c a c c t g c c g g g c c g t g a c c t c a g c t g g g c g a a g g t t t t c g g t a t t c t g g a g a a a g t a a t g g t g g a c g g c c c g g c a c t g g a g t c g a c c c g c t t c c a a a a g c c a t a a g a c c t c t t t

8520 AKEKYGVDDYSVSQISLEQV g c c a a g g a a a a g t a c g g c g t g g a c g a c t a c t c c g t g a g c c a g a t c t c g c t g g a a c a g g t c c g g t t c c t t t t c a t g c c g c a c c t g c t g a t g a g g c a c t c g g t c t a g a g c g a c c t t g t c c a g

8580 1 MV AcGFP1 FLSFAHLQPPTAEEGRPVMV t t c c t g a g c t t c g c c c a c c t g c a g c c g c c c a c c g c a g a g g a g g g g c g a c c g g t c a t g g t g a a g g a c t c g a a g c g g g t g g a c g t c g g c g g g t g g c g t c t c c t c c c c g c t g g c c a g t a c c a c

8640 5 10 15 20 SKGAELFTGIVPILIELNGD AcGFP1 SKGAELFTGIVPILIELNGD a g c a a g g g c g c c g a g c t g t t c a c c g g c a t c g t g c c c a t c c t g a t c g a g c t g a a t g g c g a t t c g t t c c c g c g g c t c g a c a a g t g g c c g t a g c a c g g g t a g g a c t a g c t c g a c t t a c c g c t a

8700 25 30 35 40 VNGHKFSVSGEGEGDATYGK AcGFP1 VNGHKFSVSGEGEGDATYGK g t g a a t g g c c a c a a g t t c a g c g t g a g c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c a c t t a c c g g t g t t c a a g t c g c a c t c g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c

8760 45 50 55 60 LTLKFICTTGKLPVPWPTLV AcGFP1 LTLKFICTTGKLPVPWPTLV c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c t g t g c c c t g g c c c a c c c t g g t g g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g a c a c g g g a c c g g g t g g g a c c a c

8820 65 70 75 80 TTLSYGVQCFSRYPDHMKQH AcGFP1 TTLSYGVQCFSRYPDHMKQH a c c a c c c t g a g c t a c g g c g t g c a g t g c t t c t c a c g c t a c c c c g a t c a c a t g a a g c a g c a c t g g t g g g a c t c g a t g c c g c a c g t c a c g a a g a g t g c g a t g g g g c t a g t g t a c t t c g t c g t g

8880 85 90 95 100 DFFKSAMPEGYIQERTIFFE AcGFP1 DFFKSAMPEGYIQERTIFFE g a c t t c t t c a a g a g c g c c a t g c c t g a g g g c t a c a t c c a g g a g c g c a c c a t c t t c t t c g a g c t g a a g a a g t t c t c g c g g t a c g g a c t c c c g a t g t a g g t c c t c g c g t g g t a g a a g a a g c t c

8940 105 110 115 120 DDGNYKSRAEVKFEGDTLVN AcGFP1 DDGNYKSRAEVKFEGDTLVN g a t g a c g g c a a c t a c a a g t c g c g c g c c g a g g t g a a g t t c g a g g g c g a t a c c c t g g t g a a t c t a c t g c c g t t g a t g t t c a g c g c g c g g c t c c a c t t c a a g c t c c c g c t a t g g g a c c a c t t a

9000 125 130 135 140 RIELTGTDFKEDGNILGNKM AcGFP1 RIELTGTDFKEDGNILGNKM c g c a t c g a g c t g a c c g g c a c c g a t t t c a a g g a g g a t g g c a a c a t c c t g g g c a a t a a g a t g g c g t a g c t c g a c t g g c c g t g g c t a a a g t t c c t c c t a c c g t t g t a g g a c c c g t t a t t c t a c

9060 145 150 155 160 EYNYNAHNVYIMTDKAKNGI AcGFP1 EYNYNAHNVYIMTDKAKNGI g a g t a c a a c t a c a a c g c c c a c a a t g t g t a c a t c a t g a c c g a c a a g g c c a a g a a t g g c a t c c t c a t g t t g a t g t t g c g g g t g t t a c a c a t g t a g t a c t g g c t g t t c c g g t t c t t a c c g t a g

9120 165 170 175 180 KVNFKIRHNIEDGSVQLADH AcGFP1 KVNFKIRHNIEDGSVQLADH a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a t g g c a g c g t g c a g c t g g c c g a c c a c t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t a c c g t c g c a c g t c g a c c g g c t g g t g

9180 185 190 195 200 YQQNTPIGDGPVLLPDNHYL AcGFP1 YQQNTPIGDGPVLLPDNHYL t a c c a g c a g a a t a c c c c c a t c g g c g a t g g c c c t g t g c t g c t g c c c g a t a a c c a c t a c c t g a t g g t c g t c t t a t g g g g g t a g c c g c t a c c g g g a c a c g a c g a c g g g c t a t t g g t g a t g g a c

9240 205 210 215 220 STQSALSKDPNEKRDHMIYF AcGFP1 STQSALSKDPNEKRDHMIYF t c c a c c c a g a g c g c c c t g t c c a a g g a c c c c a a c g a g a a g c g c g a t c a c a t g a t c t a c t t c a g g t g g g t c t c g c g g g a c a g g t t c c t g g g g t t g c t c t t c g c g c t a g t g t a c t a g a t g a a g

9300 225 230 235 240 GFVTAAAITHGMDELYK* AcGFP1 GFVTAAAITHGMDELYK* g g c t t c g t g a c c g c c g c c g c c a t c a c c c a c g g c a t g g a t g a g c t g t a c a a g t g a g c g g c c c c g a a g c a c t g g c g g c g g c g g t a g t g g g t g c c g t a c c t a c t c g a c a t g t t c a c t c g c c g g

9360 WPRE A WPRE-R g a t c c t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t c t a g g a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a

9420 WPRE C A A C G A G G A A A A T G C G A T A C WPRE-R MPLYHAIA MLLL g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a

9480 WPRE SRMAFIFSSLYKSWLLSLYE PVWLSFSPPCINPGCCLFMR t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c

9540 WPRE ELWPVVRQRGVVCTVFADAT SCGPLSGNVAWCALCLLTQP g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g

9600 WPRE PTGWGIATTCQLLSGTFAFP PLVGALPPPVSSFPGLSLSP c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g

9660 WPRE LPIATAELIAACLARCWTGA SLLPRRNSSPPALPAAGQGL c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a

9720 WPRE (in frame with Factor Xa site) *RGKRP RLLGTDNSVVLSGKSSSFPW GCWALTIPWCCRGNHRPFLG c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c

9780 WPRE (in frame with Factor Xa site) QEGTNGGPNQAPRGEAVDR R Factor Xa site LLACVATWILRGTSFCYVPS CSPVLPPGFCAGRPSATSLR c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c

9840 WPRE 1 GEI Factor Xa site ALNPADLPSRGLLPALRPLP PSIQRTFLPAACCRLCGLFR g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c

9900 WPRE RLRLRPQTSRISLWAASPPE VFAFALRRVGSPFGPPPRLR c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g a g g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c t c

9960 IL* SFKTNDLQGSCRS* a t c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a t a g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t

10,020 3' LTR ( Δ U3) a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t

10,080 3' LTR ( Δ U3) c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g

10,140 3' LTR ( Δ U3) c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a

10,200 3' LTR ( Δ U3) t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t

PsiI 10,260 3' LTR ( Δ U3) g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a

PacI 10,320 c a g a g a g t g a g a g g c c c g g g t t a a t t a a g g a a a g g g c t a g a t c a t t c t t g a a g a c g a a a g g t c t c t c a c t c t c c g g g c c c a a t t a a t t c c t t t c c c g a t c t a g t a a g a a c t t c t g c t t t c

10,380 C G G A G C A C T A T G C G G A T A A pBRforEco g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c

10,440 AmpR promoter t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t

SspI 10,500 AmpR promoter c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t

10,560 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t

10,620 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a

10,680 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c

10,740 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C G Amp-R SFRPEERFPMMSTFKVLLCG a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g

10,800 80 85 90 95 AVLSRVDAGQEQLGRRIHYS AmpR C G C C A T A A T A Amp-R AVLSRVDAGQEQLGRRIHYS g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a

10,860 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t

10,920 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a

PvuI 10,980 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a

11,040 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a

11,100 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t

11,160 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t

11,220 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a

11,280 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g

11,340 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a

11,400 280 285 EIGASLIKHW* AmpR EIGASLIKHW* g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t

11,460 c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a

11,520 g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g

11,580 ori g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c

11,640 ori c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a

11,700 ori c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c

11,760 ori t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c

11,820 ori c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g

11,880 ori t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t

11,940 ori c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t

12,000 ori g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g

12,060 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a

DrdI 12,120 ori g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c

12,180 ori a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a

12,240 t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g

12,300 A G C G A G T C A G T G A G C L4440 t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g

12,360 G A G L4440 g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a

12,420 t a a t g c a g c a a g c t c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t a t t a c g t c g t t c g a g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a

AvrII 12,480 c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t

12,540 a a a a g c t c c c c g t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a t t t t c g a g g g g c a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t

12,600 -35 CAP binding site lac promoter a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a

12,660 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c a t g g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g t a c

12,720 G T G G T T T G T C C A A A EBV-rev a t t a c g a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a t a a t g c t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t

12,780 C T C A T C EBV-rev c t c a t c a a t g t a t c t t a t c a t g t c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a a t c a t g a g t a g t t a c a t a g a a t a g t a c a g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t t a g t a

12,840 c c c a a a c t t c c c a c c c c a t a c c c t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a c t c g g g t t t g a a g g g t g g g g t a t g g g a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a a t g a g

12,900 t a a a c c t g t g a t t c c t c t g a a t t a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a t a t g t a t t t g g a c a c t a a g g a g a c t t a a t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t a t a c a

3ʹ 5ʹ 12,917 a c t a c a a a c t t a g t a g t t g a t g t t t g a a t c a t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.