Addgene pCDF-EcAvs4 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCDF-EcAvs4 Sequencing Result


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SfoI (8138) NarI * (8137) KasI (8136) BsmBI - Esp3I (8112) HpaI (8003) ApaI (7708) PspOMI (7704) MluI (7497) LacI-R (7166 .. 7185) T7 (7004 .. 7023) T7 promoter SacII (6216) BstZ17I (6178) AccI (6177) FspI - FspAI (6112) PaqCI (5975) SmaI (5893) TspMI - XmaI (5891) AlwNI (5226) BmgBI (4914) Bpu10I (4904) EagI (4898) BspEI (4586) PstI (4553) PluTI (8140) XbaI (129) SgrAI (867) PciI (894) NheI (994) BmtI (998) BspHI (1041) SspI (1076) BglI (1724) PflFI - Tth111I (1937) DrdI (1940) Bsu36I (2044) T7 Term (2099 .. 2117) AvrII (2127) NruI (2423) BspQI - SapI (2444) PpuMI (2649) SphI (2796) BseRI (3448) PshAI (3538) BsaBI (3803) BglII (3966) pCDF-EcAvs4 8288 bp

Sequence View

5ʹ 3ʹ 60 c c t t g a g a g c c t t c a a c c c a g t c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t a a c a t g a g g a a c t c t c g g a a g t t g g g t c a g t c g a g g a a g g c c a c c c g c g c c c c g t a c t g a t t g t a c t

120 g a a t t a c a a c t t a t a t c g t a t g g g g c t g a c t t c a g g t g c t a c a t t t g a a g a g a t a a a t t g c t t a a t g t t g a a t a t a g c a t a c c c c g a c t g a a g t c c a c g a t g t a a a c t t c t c t a t t t a a c

XbaI 180 c a c t g a a a t c t a g a g c g g t t c a g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t g t g a c t t t a g a t c t c g c c a a g t c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a

240 t c t g c g c g t a a t c t t t t g c c c t g t a a a c g a a a a a a c c a c c t g g g g a g g t g g t t t g a t c g a a g a c g c g c a t t a g a a a a c g g g a c a t t t g c t t t t t t g g t g g a c c c c t c c a c c a a a c t a g c t

300 a g g t t a a g c a g t t c c c c a a c t g a c t t a a c c t t c g a a a a a a c c a c c t g g g g a g g t g g t t t g t c c a a t t c g t c a a g g g g t t g a c t g a a t t g g a a g c t t t t t t g g t g g a c c c c t c c a c c a a a c

360 a t c g a a g g t t a a g t c a g t t g g g g a a c t g c t t a a c c g t g g t a a c t g g c t t t c g c a g a g c a c t a g c t t c c a a t t c a g t c a a c c c c t t g a c g a a t t g g c a c c a t t g a c c g a a a g c g t c t c g t g

420 a g c a a c c a a a t c t g t c c t t c c a g t g t a g c c g g a c t t t g g c g c a c a c t t c a a g a g c a a c c g t c g t t g g t t t a g a c a g g a a g g t c a c a t c g g c c t g a a a c c g c g t g t g a a g t t c t c g t t g g c

480 c g t g t t t a g c t a a a c a a a t c c t c t g c g a a c t c c c a g t t a c c a a t g g c t g c t g c c a g t g g c g c a c a a a t c g a t t t g t t t a g g a g a c g c t t g a g g g t c a a t g g t t a c c g a c g a c g g t c a c c g

540 g t t t t a c c g t g c t t t t c c g g g t t g g a c t c a a g t g a a c a g t t a c c g g a t a a g g c g c a g c a g c a a a a t g g c a c g a a a a g g c c c a a c c t g a g t t c a c t t g t c a a t g g c c t a t t c c g c g t c g t c

600 t c g g g c t g a a c g g g g a g t t c t t g c t t a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a g a g c c c g a c t t g c c c c t c a a g a a c g a a t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t c

660 c c g a g a t a c c a g t g t g t g a g c t a t g a g a a a g c g c c a c a c t t c c c g t a a g g g a g a a a g g c g g g c t c t a t g g t c a c a c a c t c g a t a c t c t t t c g c g g t g t g a a g g g c a t t c c c t c t t t c c g c

720 g a a c a g g t a t c c g g t a a a c g g c a g g g t c g g a a c a g g a g a g c g c a a g a g g g a g c g a c c c g c c t t g t c c a t a g g c c a t t t g c c g t c c c a g c c t t g t c c t c t c g c g t t c t c c c t c g c t g g g c g

780 c g g a a a c g g t g g g g a t c t t t a a g t c c t g t c g g g t t t c g c c c g t a c t g t c a g a t t c a t g g t g c c t t t g c c a c c c c t a g a a a t t c a g g a c a g c c c a a a g c g g g c a t g a c a g t c t a a g t a c c a

840 t g a g c c t c a c g g c t c c c a c a g a t g c a c c g g a a a a g c g t c t g t t t a t g t g a a c t c t g g c a g a c t c g g a g t g c c g a g g g t g t c t a c g t g g c c t t t t c g c a g a c a a a t a c a c t t g a g a c c g t c

PciISgrAI 900 g a g g g c g g a g c c t a t g g a a a a a c g c c a c c g g c g c g g c c c t g c t g t t t t g c c t c a c a t g t t c t c c c g c c t c g g a t a c c t t t t t g c g g t g g c c g c g c c g g g a c g a c a a a a c g g a g t g t a c a a

960 a g t c c c c t g c t t a t c c a c g g a a t c t g t g g g t a a c t t t g t a t g t g t c c g c a g c g c c c g c c g t c a g g g g a c g a a t a g g t g c c t t a g a c a c c c a t t g a a a c a t a c a c a g g c g t c g c g g g c g g c

BmtINheI 1020 AmpR promoter c a g t c t c a c g c c c g g a g c g t a g c g a c c g a g t g a g c t a g c t a t t t g t t t a t t t t t c t a a a t g t c a g a g t g c g g g c c t c g c a t c g c t g g c t c a c t c g a t c g a t a a a c a a a t a a a a a g a t t t a

SspIBspHI 1080 AmpR promoter a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c

1140 1 5 10 15 MREAVIAEVSTQLSEV AmpR promoter SmR MREAVIAEVSTQLSEV a a a a a g g a a g a g t a t g a g g g a a g c g g t g a t c g c c g a a g t a t c g a c t c a a c t a t c a g a g g t t t t t t c c t t c t c a t a c t c c c t t c g c c a c t a g c g g c t t c a t a g c t g a g t t g a t a g t c t c c a

1200 20 25 30 35 VGVIERHLEPTLLAVHLYGS SmR VGVIERHLEPTLLAVHLYGS a g t t g g c g t c a t c g a g c g c c a t c t c g a a c c g a c g t t g c t g g c c g t a c a t t t g t a c g g c t c t c a a c c g c a g t a g c t c g c g g t a g a g c t t g g c t g c a a c g a c c g g c a t g t a a a c a t g c c g a g

1260 40 45 50 55 AVDGGLKPHSDIDLLVTVTV SmR AVDGGLKPHSDIDLLVTVTV c g c a g t g g a t g g c g g c c t g a a g c c a c a c a g t g a t a t t g a t t t g c t g g t t a c g g t g a c c g t g c g t c a c c t a c c g c c g g a c t t c g g t g t g t c a c t a t a a c t a a a c g a c c a a t g c c a c t g g c a

1320 60 65 70 75 RLDETTRRALINDLLETSAS SmR RLDETTRRALINDLLETSAS a a g g c t t g a t g a a a c a a c g c g g c g a g c t t t g a t c a a c g a c c t t t t g g a a a c t t c g g c t t c t t c c g a a c t a c t t t g t t g c g c c g c t c g a a a c t a g t t g c t g g a a a a c c t t t g a a g c c g a a g

1380 80 85 90 95 PGESEILRAVEVTIVVHDDI SmR PGESEILRAVEVTIVVHDDI c c c t g g a g a g a g c g a g a t t c t c c g c g c t g t a g a a g t c a c c a t t g t t g t g c a c g a c g a c a t g g g a c c t c t c t c g c t c t a a g a g g c g c g a c a t c t t c a g t g g t a a c a a c a c g t g c t g c t g t a

1440 100 105 110 115 IPWRYPAKRELQFGEWQRND SmR IPWRYPAKRELQFGEWQRND c a t t c c g t g g c g t t a t c c a g c t a a g c g c g a a c t g c a a t t t g g a g a a t g g c a g c g c a a t g a g t a a g g c a c c g c a a t a g g t c g a t t c g c g c t t g a c g t t a a a c c t c t t a c c g t c g c g t t a c t

1500 120 125 130 135 ILAGIFEPATIDIDLAILLT SmR ILAGIFEPATIDIDLAILLT c a t t c t t g c a g g t a t c t t c g a g c c a g c c a c g a t c g a c a t t g a t c t g g c t a t c t t g c t g a c g t a a g a a c g t c c a t a g a a g c t c g g t c g g t g c t a g c t g t a a c t a g a c c g a t a g a a c g a c t g

1560 140 145 150 155 KAREHSVALVGPAAEELFDP SmR KAREHSVALVGPAAEELFDP a a a a g c a a g a g a a c a t a g c g t t g c c t t g g t a g g t c c a g c g g c g g a g g a a c t c t t t g a t c c t t t t c g t t c t c t t g t a t c g c a a c g g a a c c a t c c a g g t c g c c g c c t c c t t g a g a a a c t a g g

1620 160 165 170 175 VPEQDLFEALNETLTLWNSP SmR VPEQDLFEALNETLTLWNSP g g t t c c t g a a c a g g a t c t a t t t g a g g c g c t a a a t g a a a c c t t a a c g c t a t g g a a c t c g c c c c a a g g a c t t g t c c t a g a t a a a c t c c g c g a t t t a c t t t g g a a t t g c g a t a c c t t g a g c g g

1680 180 185 190 195 PDWAGDERNVVLTLSRIWYS SmR PDWAGDERNVVLTLSRIWYS g c c c g a c t g g g c t g g c g a t g a g c g a a a t g t a g t g c t t a c g t t g t c c c g c a t t t g g t a c a g c g g g c t g a c c c g a c c g c t a c t c g c t t t a c a t c a c g a a t g c a a c a g g g c g t a a a c c a t g t c

BglI 1740 200 205 210 215 AVTGKIAPKDVAADWAMERL SmR AVTGKIAPKDVAADWAMERL c g c a g t a a c c g g c a a a a t c g c g c c g a a g g a t g t c g c t g c c g a c t g g g c a a t g g a g c g c c t g c g t c a t t g g c c g t t t t a g c g c g g c t t c c t a c a g c g a c g g c t g a c c c g t t a c c t c g c g g a

1800 220 225 230 235 PAQYQPVILEARQAYLGQEE SmR PAQYQPVILEARQAYLGQEE g c c g g c c c a g t a t c a g c c c g t c a t a c t t g a a g c t a g a c a g g c t t a t c t t g g a c a a g a a g a c g g c c g g g t c a t a g t c g g g c a g t a t g a a c t t c g a t c t g t c c g a a t a g a a c c t g t t c t t c t

1860 240 245 250 255 DRLASRADQLEEFVHYVKGE SmR DRLASRADQLEEFVHYVKGE a g a t c g c t t g g c c t c g c g c g c a g a t c a g t t g g a a g a a t t t g t c c a c t a c g t g a a a g g c g a t c t a g c g a a c c g g a g c g c g c g t c t a g t c a a c c t t c t t a a a c a g g t g a t g c a c t t t c c g c t

1920 260 ITKVVGK* SmR ITKVVGK* g a t c a c c a a g g t a g t c g g c a a a t a a t g t c t a a c a a t t c g t t c a a g c c g a g g g g c c g c a a g c t a g t g g t t c c a t c a g c c g t t t a t t a c a g a t t g t t a a g c a a g t t c g g c t c c c c g g c g t t c

Tth111IPflFIDrdI 1980 a t c c g g c c a c g a t g a c c c g g t c g t c g g t t c a g g g c a g g g t c g t t a a a t a g c c g c t t a t g t t a g g c c g g t g c t a c t g g g c c a g c a g c c a a g t c c c g t c c c a g c a a t t t a t c g g c g a a t a c a

2040 c t a t t g c t g g t t t a c c g g t t t a t t g a c t a c c g g a a g c a g t g t g a c c g t g t g c t t c t c a a a g a t a a c g a c c a a a t g g c c a a a t a a c t g a t g g c c t t c g t c a c a c t g g c a c a c g a a g a g t t t

Bsu36I 2100 T7 terminator G C T7 Term t g c c t g a g g t t t c a g c a a a a a a c c c c t c a a g a c c c g t t t a g a g g c c c c a a g g g g t t a t g c a c g g a c t c c a a a g t c g t t t t t t g g g g a g t t c t g g g c a a a t c t c c g g g g t t c c c c a a t a c g

AvrII 2160 *GSS 5 HHHH T7 terminator 6xHis T A G T T A T T G C T C A G C G G T7 Term MM *GSSHHHH t a g t t a t t g c t c a g c g g t g g c a g c a g c c t a g g t t a a t t a g c c g c t g c t g t g g t g a t g a t g a t c a a t a a c g a g t c g c c a c c g t c g t c g g a t c c a a t t a a t c g g c g a c g a c a c c a c t a c t a c

2220 1 HH 6xHis VMASAQNVLTAHIANHATFF HHSGGLINERSMYGVVSGKE g t g a t g g c t t c c g c c c a g a a t g t t c t c a c g g c t c a t a t a g c c a a c c a c g c t a c c t t t t t c c a c t a c c g a a g g c g g g t c t t a c a a g a g t g c c g a g t a t a t c g g t t g g t g c g a t g g a a a a a g

2280 NQEVQDHQNLLFQLLGTTNT VLFDLIVLVEQKLQKTRRIR a a c c a g g a a g t c c a g g a t c a c c a g a a c c t c t t g t t t c a g c t g c t t g g t a c g a c g a a t a c g t t g g t c c t t c a g g t c c t a g t g g t c t t g g a g a a c a a a g t c g a c g a a c c a t g c t g c t t a t g c

2340 FTLVVDVTTHQAFQIVNGIV ERENNIYRRVLCELYYVTDN t t c a c g c t c g t t g t t g a t g t a a c g a c g c a c c a g g c a t t c c a g a t a g t a a a c g g t a t c g t t a a g t g c g a g c a a c a a c t a c a t t g c t g c g t g g t c c g t a a g g t c t a t c a t t t g c c a t a g c a a

2400 FQFLLPQFLVHAQNLAQPGD ELKKEWLKKNVSLIESLWTI t t c c a g t t t c t t c t c c c a c a g t t t c t t g t t c a c g c t c a g a a t c t c g c t c a g c c a g g t g a t a a g g t c a a a g a a g a g g g t g t c a a a g a a c a a g t g c g a g t c t t a g a g c g a g t c g g t c c a c t a

BspQISapINruI 2460 TLVQVAARDVVQALRQAIQG GQNLYCSAINNLSKALSYLT a c c c t g g t t c a g g t a g c a g c t c g c g a t g t t g t t c a g g c t c t t c g c c a g g c t a t a c a g g g t t g g g a c c a a g t c c a t c g t c g a g c g c t a c a a c a a g t c c g a g a a g c g g t c c g a t a t g t c c c a

2520 ARAVTHGTANIAKELAVAVF SPCHGMTRSVDCFFQSNSDK g c t c g g g c a g t g a c c c a t g g t a c g g c t a a c a t c g c a a a a g a a t t g g c t g t t g c t g t c t t t c g a g c c c g t c a c t g g g t a c c a t g c c g a t t g t a g c g t t t t c t t a a c c g a c a a c g a c a g a a a

2580 KGVPAVAVFLPRALREQVAF FTHWGNSNEKWPSEAFLYSK a a a g g t g t g c c a g c c g t t g c t g t t t t c c t t c c a c g g g c t c t c c g c g a a c a g g t a g c t t t t t t t c c a c a c g g t c g g c a a c g a c a a a a g g a a g g t g c c c g a g a g g c g c t t g t c c a t c g a a a a

2640 NDLVNVPVTVAIFAQGNHLV IIKDVYWYRDGDKCLTVVKD a a t g a t c t t g t c a a c g t a c c a g t a a c g g t c g c c a t c t t t g c a c a g g g t a a c c a c c t t g t c t t a c t a g a a c a g t t g c a t g g t c a t t g c c a g c g g t a g a a a c g t g t c c c a t t g g t g g a a c a g

PpuMI 2700 EEQVPNFPELRIGVQTVLHQ FFLDWVKWFKAYTNLRDEVL g a a g a a c a g g t c c c a a a c t t t c c a g a a c t t c g c a t a g g t g t t c a g a c g g t c c t c c a c c a g c t t c t t g t c c a g g g t t t g a a a g g t c t t g a a g c g t a t c c a c a a g t c t g c c a g g a g g t g g t c

2760 NKFLEQFADRLAAVKTIQER IFKKFLESIPESGNFGDLFP a a t a a a t t t c t t g a a c a g t t c g c t g a t c g g c t c g c t g c c g t t a a a a c c a t c c a g g a a c g g t t a t t t a a a g a a c t t g t c a a g c g a c t a g c c g a g c g a c g g c a a t t t t g g t a g g t c c t t g c c

SphI 2820 FNVVRDVANRRMQGKVRETF KIYDPIDSVPAHLTFYAFRE t t t a a t g t a g t c c g g g a t g t c g c t a a c c g g c g c a t g c a g g g t a a a g t a c g c g a a a c g t t c a a a t t a c a t c a g g c c c t a c a g c g a t t g g c c g c g t a c g t c c c a t t t c a t g c g c t t t g c a a g

2880 QKALTQRIVHTILTNAAQQA LFSQRLAYDVRDERVSSLLS c a g a a a g c t c t g a c g c a g c g c a t a g t c c a c a c g a t c c t c a c g a a c g c t g c t c a g c a g g c t g t c t t t c g a g a c t g c g t c g c g t a t c a g g t g t g c t a g g a g t g c t t g c g a c g a g t c g t c c g a

2940 GAETLHDQANQFLVLNVHGV TSFRKVILSVLKKHEINVTD g g t g c t g a a a c g c t t c a c g a t c a g g c t a a c c a g t t t c t t g t g c t c a a t g t t c a c g g t g t c c c a c g a c t t t g c g a a g t g c t a g t c c g a t t g g t c a a a g a a c a c g a g t t a c a a g t g c c a c a g

3000 NRDQLKRGVQNVQVNFIHAT VPILQFATNLIHLDVKDMSG a a c c g g g a t c a g t t g a a a c g c g g t g t t c a g a a t g t g c a g g t c a a c t t t a t c c a t g c t a c c t t g g c c c t a g t c a a c t t t g c g c c a c a a g t c t t a c a c g t c c a g t t g a a a t a g g t a c g a t g g

3060 QIVNADLVINNVADAVFIVV LDDISIKNDIVNSICHKYNN c a g a t c g t c a a t g c t g a t c t t g t t a t c a a t a a c g t t g c t g a t g c a g t g t t t a t a g t t g t t g t c t a g c a g t t a c g a c t a g a a c a a t a g t t a t t g c a a c g a c t a c g t c a c a a a t a t c a a c a a

3120 QEHLVDVDFLDVVINLTVAF LFVKNINIKKIDYDVQRYSE c a g g a a c a c c t t g t t g a t g t t g a t t t t c t t g a t g t c g t a a t c a a c c t g a c g g t a g c t t t c g t c c t t g t g g a a c a a c t a c a a c t a a a a g a a c t a c a g c a t t a g t t g g a c t g c c a t c g a a a g

3180 VNDFTAQNHVFRLQDQKTEQ HIIKRSLIVYKPKLILFGFL g t g a a t g a t t t t a c g g c t c a g a a t c a c g t a t t t c g g c t t c a g g a t c a g a a a a c c g a a c a g c a c t t a c t a a a a t g c c g a g t c t t a g t g c a t a a a g c c g a a g t c c t a g t c t t t t g g c t t g t c

3240 QALHIVKVVQPQLVNHHARE LSQMDDFYDLWLKHIVMSPF c a g g c t t t g c a t a t c g t c a a a g t a g t c c a g c c a c a g c t t g t g a a t c a c c a t g c t c g g g a a g t c c g a a a c g t a t a g c a g t t t c a t c a g g t c g g t g t c g a a c a c t t a g t g g t a c g a g c c c t t

3300 NAITATRHADAVIVEQGQQQ VSYRGGAMGISHDNFLTLLL a a c g c t a t a a c g g c c a c c c g c c a t g c c g a t g c t g t g a t c g t t g a a c a g g g t c a g c a g c a g t t g c g a t a t t g c c g g t g g g c g g t a c g g c t a c g a c a c t a g c a a c t t g t c c c a g t c g t c g t c

3360 NGFDGFTLHRVVVINNRQRA ITKITEREMPYNHYIVPLAS a a t g g t t t t g a t g g t t t c a c g c t c c a t c g g g t a g t t g t g a t a a a t a a c c g g c a g c g c g c t t t a c c a a a a c t a c c a a a g t g c g a g g t a g c c c a t c a a c a c t a t t t a t t g g c c g t c g c g c g a

3420 DRAGGTILHLIVVTFFQRQL IASTTGDQVQYNYGEKLPLK g a t c g c g c t g g t g g t a c c a t c t t g c a c c t g a t a g t t g t a a c c t t c t t t c a g c g g c a g c t t c t a g c g c g a c c a c c a t g g t a g a a c g t g g a c t a t c a a c a t t g g a a g a a a g t c g c c g t c g a a

BseRI 3480 AVRQNNILAILTLLVVQLVK SYALVIDKCYEREENNLQNF g c t g t a c g c c a g a a c a a t a t c c t t g c a a t a c t c a c g c t c c t c g t t g t t c a g c t g g t t a a a c g a c a t g c g g t c t t g t t a t a g g a a c g t t a t g a g t g c g a g g a g c a a c a a g t c g a c c a a t t t

PshAI 3540 VIQQQHAANVRRDVTVQQTE YDLLLVSCVDAPINGNLLRF g t a a t c c a g c a g c a g c a c g c t g c a a a c g t c c g c c g g g a t g t t a c c g t t c a g c a g a c g g a a c a t t a g g t c g t c g t c g t g c g a c g t t t g c a g g c g g c c c t a c a a t g g c a a g t c g t c t g c c t t

3600 VFATFLVQFLNDFFGLLQRN DEGGEENLKKIIEKTEQLAI g t c t t c g c c a c c t t c c t c g t t c a g t t t c t t a a t g a t t t c t t t g g t c t c t t g c a g c g c a a t c a g a a g c g g t g g a a g g a g c a a g t c a a a g a a t t a c t a a a g a a a c c a g a g a a c g t c g c g t t a

3660 LRVILVHAIVLVTFVFLTLV QPNDEYMGYNKYREDKERKY c t g c g g g t t a t c c t c g t a c a t g c c a t a g t t c t t g t a a c g t t c g t c t t t c t c a c g c t t g t a g a c g c c c a a t a g g a g c a t g t a c g g t a t c a a g a a c a t t g c a a g c a g a a a g a g t g c g a a c a t

3720 ARPQLQGHVLHVFAVFLNRF SAWLKLTVYKMHESNKKIAE g c t c g c c c a c a g c t t c a g g g t c a c g t a c t t c a t g t g t t c g c t g t t t t t c t t a a t c g c t t c c g a g c g g g t g t c g a a g t c c c a g t g c a t g a a g t a c a c a a g c g a c a a a a a g a a t t a g c g a a g

3780 LAVLFQLRVDLRVEANFDTL ESYQKLKPDIEPNFSIEIGE c t c g c t g t a c t g t t t c a g c t t c g g g t c g a t c t c c g g g t t g a a g c t a a t t t c g a t a c c c t c g a g c g a c a t g a c a a a g t c g a a g c c c a g c t a g a g g c c c a a c t t c g a t t a a a g c t a t g g g a g

BsaBI 3840 IFFLGGDFHLTTVHTRQAQT DKEKTTIKMKRRDMRALCLR a t c t t t t t c c t t g g t g g t g a t t t t c a t c t t a c g a c g g t c c a t a c g c g c c a g g c a c a g a c g t a g a a a a a g g a a c c a c c a c t a a a a g t a g a a t g c t g c c a g g t a t g c g c g g t c c g t g t c t g c

3900 PGFIRFGLLRFVRQLVIVFV WTKDAETEQAEDPLQNYYKD c c a g g t t t t a t c c g c t t c g g t c t c t t g c g c t t c g t c c g g c a g c t g g t t a t a g t a t t t g t c g g t c c a a a a t a g g c g a a g c c a g a g a a c g c g a a g c a g g c c g t c g a c c a a t a t c a t a a a c a g

3960 KNIPQHLLTLDRVLFGNVLA FIDWLVQQREIADKETVNES a a a a a t a t c c c a c a g c a c t t g c t g a c g c t c g a t c g c g t c c t t t t c g g t a a c g t t c t c g c t t t t t t a t a g g g t g t c g t g a a c g a c t g c g a g c t a g c g c a g g a a a a g c c a t t g c a a g a g c g a

BglII 4020 TEDLVVQRQVLQIGVTVNIV RFIQYHLALNELYTNRHVDD a c g g a a g a t c t g g t a g t g c a g c g c c a g g t t c t c c a g a t a g g t g t t a c g g t g a a c a t c g t c t g c c t t c t a g a c c a t c a c g t c g c g g t c c a a g a g g t c t a t c c a c a a t g c c a c t t g t a g c a g

4080 ARFNTVFLVQAVTVIGATEA CAKIRDEEHLSNRYDTGGFG g c a c g c t t t a a t a c g g t c t t c c t c g t g c a g g c t g t t a c g g t a a t c g g t g c c a c c g a a g c c c g t g c g a a a t t a t g c c a g a a g g a g c a c g t c c g a c a a t g c c a t t a g c c a c g g t g g c t t c g g

4140 VTQADQAALVGTIQHHTVHI DRLSILSSKHTRDLVMRNMD g t c a c g c a g g c t g a t c a g g c t g c t c t t g t g g g t a c g a t c c a g c a c c a t a c g g t t c a t a t c c a g t g c g t c c g a c t a g t c c g a c g a g a a c a c c c a t g c t a g g t c g t g g t a t g c c a a g t a t a g

4200 KTKEVFGLEQHLRNVKGFFR FRFFDKTQFLVKAVNFTKEP a a a a c g a a a g a a g t c t t t g g t t t g g a a c a g c a c c t t c g c a a c g t t a a a g g t t t t t t c c g g t t t t g c t t t c t t c a g a a a c c a a a c c t t g t c g t g g a a g c g t t g c a a t t t c c a a a a a a g g c c

4260 ERQNNAGNHRADARAFGVTQ FALVISTVVASISASKTNRL g a a c g c c a g a a c a a t g c t g g t a a c c a c c g c g c t g a t g c t c g c g c t t t t g g t g t t a c g c a g c t t g c g g t c t t g t t a c g a c c a t t g g t g g c g c g a c t a c g a g c g c g a a a a c c a c a a t g c g t c

4320 QKQQPALQHLAVRVFEVFQK LFLLWSELVKSDANKFNELF c a g a a a c a g c a g c c a g c t c t c c a g c a c c t t g c t g t c c g c g t t t t t g a a g t t t t c c a g a a a g t c t t t g t c g t c g g t c g a g a g g t c g t g g a a c g a c a g g c g c a a a a a c t t c a a a a g g t c t t t

4380 ELLQRHVDAAQQVGANLGTT FKELAMHISSLLYTSVQTGR g a a c t t c t c c a g c g c c a t g t g g a t g c t g c t c a g c a g g t a g g t g c t a a c t t g g g t a c c a c g c t t g a a g a g g t c g c g g t a c a c c t a c g a c g a g t c g t c c a t c c a c g a t t g a a c c c a t g g t g c

4440 IAAPQTVANVLFDKFAFLNE YSCWLRNCIYQKIFKGEEIF a t a g c t g c a c c a c a g a c g g t t g c a a a t g t a c t g t t t g a t a a a t t t g c c t t c c t c a a t g a a t a t c g a c g t g g t g t c t g c c a a c g t t t a c a t g a c a a a c t a t t t a a a c g g a a g g a g t t a c t t

4500 HINFLDLVFRKVAVREADGG VDVEEIENKAFHSHAFCITT c a c a t c a a c t t c c t c g a t c t c g t t t t t c g c a a a g t g g c t g t g c g c g a a g c a g a t g g t g g t g t g t a g t t g a a g g a g c t a g a g c a a a a a g c g t t t c a c c g a c a c g c g c t t c g t c t a c c a c c a

PstI 4560 LVGKVQNEIDGFLELALQQP KNTFDLIFDITKKFQSQLLW c t t g t t g g t a a a g t c c a g a a t g a a a t c g a t g g t t t t c t t g a a t t g g c t c t g c a g c a g c c a g a a c a a c c a t t t c a g g t c t t a c t t t a g c t a c c a a a a g a a c t t a a c c g a g a c g t c g t c g g t

BspEI 4620 IDRGLVRAFRIVVVVATKQT YIPTQYPSEPYYDHHSRFLG a t a g a t c g g g g t c t g g t a c g g g c t t t c c g g a t a g t a g t c g t g g t g g c t a c g a a a c a g a c c t a t c t a g c c c c a g a c c a t g c c c g a a a g g c c t a t c a t c a g c a c c a c c g a t g c t t t g t c t g g

4680 EFVLNIHAVVTLAGFFQRTI FEDEIDMSHYREGTEKLPRY g a a t t c g t c c t c a a t a t c c a t g c t g t g g t a a c g c t c g c c g g t t t c t t t c a g c g g a c g a t a c t t a a g c a g g a g t t a t a g g t a c g a c a c c a t t g c g a g c g g c c a a a g a a a g t c g c c t g c t a t

4740 PKQVRQLQHVLRQARPNRIA WFLDALQLVYEPLSAWVPYS c c a a a a c a g g t c c g c c a g t t g c a g c a c g t a c t c c g g c a g g c t c g c c c a a a c c g g a t a g c t g g t t t t g t c c a g g c g g t c a a c g t c g t g c a t g a g g c c g t c c g a g c g g g t t t g g c c t a t c g a

4800 VQIGQDQGHQIEIRGTVFLP DLDTLILTMLDFYPTGHEKW g t c c a g a t c g g t c a g g a t c a g g g t c a t c a g a t c g a a a t a c g g g g t a c c g t g t t c c t t c c a c a g g t c t a g c c a g t c c t a g t c c c a g t a g t c t a g c t t t a t g c c c c a t g g c a c a a g g a a g g t

4860 TVFQHLNQHFLHLRFDHRRT RNKLVKVLVEEMEPKIMAAG a c g g t t t t t c a g c a c c t t a a c c a g c a c t t c c t c c a t c t c c g g t t t g a t c a t c g c c g c a c c t g c c a a a a a g t c g t g g a a t t g g t c g t g a a g g a g g t a g a g g c c a a a c t a g t a g c g g c g t g g

BmgBIBpu10IEagI 4920 VQNGVQDVLFVITAAQVHVL HLITHLINKENDRGSLYVDE g t g c a g a a t g g t g t g c a g g a t g t t c t t t t c g t t a t c a c g g c c g c t c a g g t a c a c g t c c t c c a c g t c t t a c c a c a c g t c c t a c a a g a a a a g c a a t a g t g c c g g c g a g t c c a t g t g c a g g a g

4980 INGPLIVFQRDQAAQTGGFT DITWQYYKLAILSSLRTTEG a t c a a t g g t c c a c t g a t a g t a t t t c a g c g c g a t c a g g c t g c t c a g a c g g g t g g t t t c a c c t a g t t a c c a g g t g a c t a t c a t a a a g t c g c g c t a g t c c g a c g a g t c t g c c c a c c a a a g t g g

5040 HFVTLVPLLDNRQNKVDVTN VKNRQNWEQIVPLIFNINRI c a c t t t g t t a c g t t g g t t c c a c t c c t g g a t a a c c g g c a g a a t a a a g t t g a t g t t a c g a a t g t g a a a c a a t g c a a c c a a g g t g a g g a c c t a t t g g c c g t c t t a t t t c a a c t a c a a t g c t t a

5100 ADFVQVLINELDHALPAGTF GIEDLNEYIFQIVSQWGTGK g c c g a t t t c g t c c a g g t t c t c a t a a a t g a a t t g g a t c a c g c t c t g c c a g c c g g t a c c t t t c g g c t a a a g c a g g t c c a a g a g t a t t t a c t t a a c c t a g t g c g a g a c g g t c g g c c a t g g a a a

5160 RLGQNVLDAQQIAVAHAQLF PKTLVYKISLLDSNSVGLQK c g g c t t g g t c a g a a c g t a c t t g a t g c t c a g c a g a t c g c t g t t g c t c a c g c c c a g t t g t t t g c c g a a c c a g t c t t g c a t g a a c t a c g a g t c g t c t a g c g a c a a c g a g t g c g g g t c a a c a a a

5220 QQVIINVLARQTQQKGQTFQ LLDYDVDKCALRLLFTLRKL c a g c a g g t c a t a a t c a a c g t c c t t g c a c g c c a g a c g c a g c a g a a a g g t c a g a c g t t t c a g g t c g t c c a g t a t t a g t t g c a g g a a c g t g c g g t c t g c g t c g t c t t t c c a g t c t g c a a a g t c

AlwNI 5280 QVLVAAQQITLEVVKENAAV LNQDSSLLYRKFYNFFISSN c a g g t t c t g g t c g c t g c t c a g c a g a t a a c g c t t g a a g t a g t t a a a g a a a a t g c t g c t g t t g t c c a a g a c c a g c g a c g a g t c g t c t a t t g c g a a c t t c a t c a a t t t c t t t t a c g a c g a c a a

5340 IAQQNRHPQLVFPKVADAFT DSLLVAVWLEDKWFNSIGEG a t c g c t c a g c a g a a c c g c c a c c c a c a g c t c g t c t t t c c a a a a g t t g c t g a t g c c t t c a c c t a g c g a g t c g t c t t g g c g g t g g g t g t c g a g c a g a a a g g t t t t c a a c g a c t a c g g a a g t g g

5400 ANNLRDERLNALIVQQQTFA CVIEAIFPKISQDDLLLRES g c a a a c a a t c t c c g c g a t g a a c g g c t t a a t g c t c t g a t c g t c c a g c a g c a g a c g t t c g c t c g t t t g t t a g a g g c g c t a c t t g c c g a a t t a c g a g a c t a g c a g g t c g t c g t c t g c a a g c g a

5460 DPVTKATTNRQALADFLKEF IWNRFSRRVPLSEGIKEFFE g a t c c a g t t a c g a a a g c t a c g a c g a a c c g g c a g g c t c t c g c c g a t t t t c t c a a a g a a t t c c t a g g t c a a t g c t t t c g a t g c t g c t t g g c c g t c c g a g a g c g g c t a a a a g a g t t t c t t a a g

5520 VVVRLTNVVHADFLFQRPFL NNNAKRIYDVSIKKELAWEE g t t g t t g t t c g c c t t a c g a a t g t a g t c c a c g c t g a t t t t c t t t t c c a g c g c c c a t t c c t c c a a c a a c a a g c g g a a t g c t t a c a t c a g g t g c g a c t a a a a g a a a a g g t c g c g g g t a a g g a g

5580 INIVGDEIARRFVAHNTIFN YIDHTIFYGAAEYGVIGDKV a t a a a t a t c g t g g g t g a t g a a a t a g c c c g c c g c t t c g t a g c c c a c a a t a c c a t c t t t a a c t a t t t a t a g c a c c c a c t a c t t t a t c g g g c g g c g a a g c a t c g g g t g t t a t g g t a g a a a t t g

5640 QLVQDTGIARRAHKELAFAR LEDLIGTDCAPSVFFQGESA c a g c t c g t c c a g g a t a c c g g t a t c g c a c g c c g g g c t c a c a a a g a a t t g g c c t t c g c t c g c g t c g a g c a g g t c c t a t g g c c a t a g c g t g c g g c c c g a g t g t t t c t t a a c c g g a a g c g a g c g

5700 TLKRGRQEALLTARFVLHDD RQFATALFCQERSPKNKVII a c g c t g a a a c g c g g t c g c c a g g a a g c a c t g c t c a c g g c t c g g t t t g t t c t t c a c g a t g a t t g c g a c t t t g c g c c a g c g g t c c t t c g t g a c g a g t g c c g a g c c a a a c a a g a a g t g c t a c t a

5760 LVPQLFLEANVVNAFTGIKT KNWLKEKFSVYDISEGTYFR c t t g t t c c a c a g c t t t t c c t t g a a g c t a a c g t a g t c a a t g c t t t c a c c g g t a t a a a a a c g g a a c a a g g t g t c g a a a a g g a a c t t c g a t t g c a t c a g t t a c g a a a g t g g c c a t a t t t t t g c

5820 QVLAQIERVLDQLQQTNILR LYESLYFPNKILELLRVDQP c a g g t a c t c g c t c a g a t a g a a c g g g t t c t t g a t c a g c t c c a g c a g a c g a a c a t c t t g c g g g t c c a t g a g c g a g t c t a t c t t g c c c a a g a a c t a g t c g a g g t c g t c t g c t t g t a g a a c g c c

5880 QAETVVVAQLDQFATLQVFN LSFGNNDSLEILEGRELNKI c a g g c t g a a a c c g t t g t t g t c g c t c a g c t c g a t c a g t t c g c c a c g c t c c a g g t t t t t a a t g t c c g a c t t t g g c a a c a a c a g c g a g t c g a g c t a g t c a a g c g g t g c g a g g t c c a a a a a t t a

TspMIXmaISmaI 5940 HQVTRGDLVNIDKRIVQVRQ VLNGPTIKYIDIFAYNLDAL c a c c a g g t t a c c c g g g g t g a t c t t g t a a a t a t c g a t a a a c g c a t a g t t c a g g t c c g c c a g g t g g t c c a a t g g g c c c c a c t a g a a c a t t t a t a g c t a t t t g c g t a t c a a g t c c a g g c g g t c

PaqCI 6000 VVVTGGENHLPFILNQNGQK YNNRTTFVVQWKDKILVTLF g t a g t t g t t a c g g g t g g t g a a a a c c a c c t g c c a t t t a t c c t t a a t c a g a a c g g t c a g a a a c a t c a a c a a t g c c c a c c a c t t t t g g t g g a c g g t a a a t a g g a a t t a g t c t t g c c a g t c t t t

6060 FLERVDVGQFQQFLRAINHD EKFPDINTLELLKEASDVVI t t c c t t g a a c g g g t c g a t g t t g g t c a g t t c c a g c a g t t t c t c c g c g c t a t c a a c c a c g a t a a g g a a c t t g c c c a g c t a c a a c c a g t c a a g g t c g t c a a a g a g g c g c g a t a g t t g g t g c t a

FspAIFspI 6120 HLTQLVLVRVAKIVQAVRTE VKRLEDQHANSFDDLGHAGF c a c c t t a c g c a g c t c g t c t t g g t g c g c g t t g c t a a a a t c g t c c a g g c c g t g c g c a c c g a a g t g g a a t g c g t c g a g c a g a a c c a c g c g c a a c g a t t t t a g c a g g t c c g g c a c g c g t g g c t t

AccIBstZ17I 6180 QFVNAVFLELARFKNVERGI LENISDKKFESAKFVYFPTY c a g t t c g t t a a t g c t g t c t t t c t t g a a c t c g c t c g c t t t a a a a a c g t a g a a c g g g g t a t a g t c a a g c a a t t a c g a c a g a a a g a a c t t g a g c g a g c g a a a t t t t t g c a t c t t g c c c c a t a t

SacII 6240 LLFRLVNFLDHRGFANATLA QKEAEYIKKIVATKGVGGEG c t g c t t t t c c g c c t c g t a a a t t t t c t t g a t c a c c g c g g t t t t g c c a a c g c c a c c c t c g c c g a c g a a a a g g c g g a g c a t t t a a a a g a a c t a g t g g c g c c a a a a c g g t t g c g g t g g g a g c g g

6300 ANNHDFLLNQVFVQQLDAVT SVIVIKKQVLNEHLLEICHR g c t a a c a a t c a c g a t t t t c t t t t g a a c c a g g t t t t c g t g c a g c a g c t c g a t g c a g t g a c g c g a t t g t t a g t g c t a a a a g a a a a c t t g g t c c a a a a g c a c g t c g t c g a g c t a c g t c a c t g c

6360 TINFDATVLELNVGLDFVQH RDIEISRDKFEINTQIEYLV a c g a t c a a t t t c g a t g c t a c g g t c c t t g a a c t c a a t g t t g g t c t g g a t t t c g t a c a g c a c t g c t a g t t a a a g c t a c g a t g c c a g g a a c t t g a g t t a c a a c c a g a c c t a a a g c a t g t c g t g

6420 VLGVFTLFLQQIKDAFVQAK NETHKRKEELLDFISENLSF g t t c t c g g t g t g t t t a c g c t t t t c c t c c a g c a g a t c a a a g a t g c t t t c g t t c a g g c t a a a c a a g a g c c a c a c a a a t g c g a a a a g g a g g t c g t c t a g t t t c t a c g a a a g c a a g t c c g a t t t

6480 EVFRNLFVLNDHERALKEAR FHKAIKENEVIVFPSEFFSA g a a g t g t t t c g c a a t c t t t t c g t t c t c a a c g a t c a c g a a c g g g c t c t c a a a g a a g c t c g c c t t c a c a a a g c g t t a g a a a a g c a a g a g t t g c t a g t g c t t g c c c g a g a g t t t c t t c g a g c g

6540 HTPNNFDTALIRLLIHFNFD VRWVIEIGSEYAKQDVEIKI c a c a c g c c a a a c a a t t t c g a t a c c g c t c t c a t a c g c c t t c t g a t c c a c t t c a a t t t t g a t g t g t g c g g t t t g t t a a a g c t a t g g c g a g a g t a t g c g g a a g a c t a g g t g a a g t t a a a a c t a

6600 LRVVAVTNGLQVVVRVFIAF KPDNSNGVTELYNDANKDGE c t t c g g g t c g t t g c t g t t a c c a a c g g t c t c c a g g t a g t t g t c c g c g t t t t t a t c g c c t t c g a a g c c c a g c a a c g a c a a t g g t t g c c a g a g g t c c a t c a a c a g g c g c a a a a a t a g c g g a a g

6660 RLVALTALTPLLVGVENDFA PEHSKRGQGWEQNTYFIIKS c g g c t c g t g g c t c t t a c g g c c t t g a c c c c a c t c c t g g t t g g t g t a g a a a a t g a t t t t g c t g c c g a g c a c c g a g a a t g c c g g a a c t g g g g t g a g g a c c a a c c a c a t c t t t t a c t a a a a c g a

6720 QTRIRFLALFNHLDQVRLVI LGPYAKKSKEIMEILDAKND c a g a c c c g g a t a c g c t t t c t t g c t c t t t t c a a t c a t c t c g a t c a g g t c c g c c t t g t t a t c g t c t g g g c c t a t g c g a a a g a a c g a g a a a a g t t a g t a g a g c t a g t c c a g g c g g a a c a a t a g

6780 AQFGIVKLALPANDFVVFGN SLKTDYFKSQWGIIENDKTI g c t c a g t t t g g t a t c g t a a a a c t t g c t t t g c c a g c c a a t g a t t t c g t t g t c t t t g g t a a t c g a g t c a a a c c a t a g c a t t t t g a a c g a a a c g g t c g g t t a c t a a a g c a a c a g a a a c c a t t a

6840 RVGLDAALVFVTEDTRRHLK PNTEIGSQNKYRFIGAPMKF c g g g t t g g t c t c g a t g c c g c t c t g g t t t t t g t a a c g g a a g a t a c c c g c c g g c a t c t t a a a g c c c a a c c a g a g c t a c g g c g a g a c c a a a a a c a t t g c c t t c t a t g g g c g g c c g t a g a a t t t

6900 FLAEQQIAKPFEVTLRVLAK EQCFLLYCFWEFNGQPNESF t t c t t g g c a g a a c a g c a g a t a g c a a a a c c a t t c g a a g t t a c c c t g c g g g t t c t c g c t a a a a a g a a c c g t c t t g t c g t c t a t c g t t t t g g t a a g c t t c a a t g g g a c g c c c a a g a g c g a t t t

6960 FRLEVVPVRLHHGISPY* KAKFNDWNPKVM t t t c g c c t t g a a g t t g t c c c a g t t c g g c t t c a c c a t g g t a t a t c t c c t t a t t a a a g t t a a a a a g c g g a a c t t c a a c a g g g t c a a g c c g a a g t g g t a c c a t a t a g a g g a a t a a t t t c a a t t

7020 lac operator T7 promoter G G G A T A T C A C T C A G C A T T7 a c a a a a t t a t t t c t a c a g g g g a a t t g t t a t c c g c t c a c a a t t c c c c t a t a g t g a g t c g t a t g t t t t a a t a a a g a t g t c c c c t t a a c a a t a g g c g a g t g t t a a g g g g a t a t c a c t c a g c a t

7080 T7 promoter lacI promoter A A T T7 t t a a t t t c c t a a t g c a g g a g t c g c a t a a g g g a g a g c g t c g a g a t c c c g g a c a c c a t c g a a a a t t a a a g g a t t a c g t c c t c a g c g t a t t c c c t c t c g c a g c t c t a g g g c c t g t g g t a g c t t

7140 lacI promoter t g g c g c a a a a c c t t t c g c g g t a t g g c a t g a t a g c g c c c g g a a g a g a g t c a a t t c a g g g t g a c c g c g t t t t g g a a a g c g c c a t a c c g t a c t a t c g c g g g c c t t c t c t c a g t t a a g t c c c a c

7200 1 5 10 15 MKPVTLYDVAEYAGVSYQ lacI promoter lacI T G C T A C A G C G T C T C A T A C G G LacI-R g t g a a t g t g a a a c c a g t a a c g t t a t a c g a t g t c g c a g a g t a t g c c g g t g t c t c t t a t c a g c a c t t a c a c t t t g g t c a t t g c a a t a t g c t a c a g c g t c t c a t a c g g c c a c a g a g a a t a g t c

7260 20 25 30 35 TVSRVVNQASHVSAKTREKV lacI a c c g t t t c c c g c g t g g t g a a c c a g g c c a g c c a c g t t t c t g c g a a a a c g c g g g a a a a a g t g t g g c a a a g g g c g c a c c a c t t g g t c c g g t c g g t g c a a a g a c g c t t t t g c g c c c t t t t t c a c

7320 40 45 50 55 EAAMAELNYIPNRVAQQLAG lacI MAELNYIPNRVAQQLAG g a a g c g g c g a t g g c g g a g c t g a a t t a c a t t c c c a a c c g c g t g g c a c a a c a a c t g g c g g g c c t t c g c c g c t a c c g c c t c g a c t t a a t g t a a g g g t t g g c g c a c c g t g t t g t t g a c c g c c c g

7380 60 65 70 75 KQSLLIGVATSSLALHAPSQ lacI KQSLLIGVATSSLALHAPSQ a a a c a g t c g t t g c t g a t t g g c g t t g c c a c c t c c a g t c t g g c c c t g c a c g c g c c g t c g c a a t t t g t c a g c a a c g a c t a a c c g c a a c g g t g g a g g t c a g a c c g g g a c g t g c g c g g c a g c g t t

7440 80 85 90 95 IVAAIKSRADQLGASVVVSM lacI IVAAIKSRADQLGASVVVSM a t t g t c g c g g c g a t t a a a t c t c g c g c c g a t c a a c t g g g t g c c a g c g t g g t g g t g t c g a t g t a a c a g c g c c g c t a a t t t a g a g c g c g g c t a g t t g a c c c a c g g t c g c a c c a c c a c a g c t a c

MluI 7500 100 105 110 115 VERSGVEACKAAVHNLLAQR lacI VERSGVEACKAAVHNLLAQR g t a g a a c g a a g c g g c g t c g a a g c c t g t a a a g c g g c g g t g c a c a a t c t t c t c g c g c a a c g c c a t c t t g c t t c g c c g c a g c t t c g g a c a t t t c g c c g c c a c g t g t t a g a a g a g c g c g t t g c g

7560 120 125 130 135 VSGLIINYPLDDQDAIAVEA lacI VSGLIINYPLDDQDAIAVEA g t c a g t g g g c t g a t c a t t a a c t a t c c g c t g g a t g a c c a g g a t g c c a t t g c t g t g g a a g c t c a g t c a c c c g a c t a g t a a t t g a t a g g c g a c c t a c t g g t c c t a c g g t a a c g a c a c c t t c g a

7620 140 145 150 155 ACTNVPALFLDVSDQTPINS lacI ACTNVPALFLDVSDQTPINS g c c t g c a c t a a t g t t c c g g c g t t a t t t c t t g a t g t c t c t g a c c a g a c a c c c a t c a a c a g t c g g a c g t g a t t a c a a g g c c g c a a t a a a g a a c t a c a g a g a c t g g t c t g t g g g t a g t t g t c a

7680 160 165 170 175 IIFSHEDGTRLGVEHLVALG lacI IIFSHEDGTRLGVEHLVALG a t t a t t t t c t c c c a t g a a g a c g g t a c g c g a c t g g g c g t g g a g c a t c t g g t c g c a t t g g g t t a a t a a a a g a g g g t a c t t c t g c c a t g c g c t g a c c c g c a c c t c g t a g a c c a g c g t a a c c c a

PspOMIApaI 7740 180 185 190 195 HQQIALLAGPLSSVSARLRL lacI HQQIALLAGPLSSVSARLRL c a c c a g c a a a t c g c g c t g t t a g c g g g c c c a t t a a g t t c t g t c t c g g c g c g t c t g c g t c t g g t g g t c g t t t a g c g c g a c a a t c g c c c g g g t a a t t c a a g a c a g a g c c g c g c a g a c g c a g a c

7800 200 205 210 215 AGWHKYLTRNQIQPIAEREG lacI AGWHKYLTRNQIQPIAEREG g c t g g c t g g c a t a a a t a t c t c a c t c g c a a t c a a a t t c a g c c g a t a g c g g a a c g g g a a g g c c g a c c g a c c g t a t t t a t a g a g t g a g c g t t a g t t t a a g t c g g c t a t c g c c t t g c c c t t c c g

7860 220 225 230 235 DWSAMSGFQQTMQMLNEGIV lacI DWSAMSGFQQTMQMLNEGIV g a c t g g a g t g c c a t g t c c g g t t t t c a a c a a a c c a t g c a a a t g c t g a a t g a g g g c a t c g t t c t g a c c t c a c g g t a c a g g c c a a a a g t t g t t t g g t a c g t t t a c g a c t t a c t c c c g t a g c a a

7920 240 245 250 255 PTAMLVANDQMALGAMRAIT lacI PTAMLVANDQMALGAMRAIT c c c a c t g c g a t g c t g g t t g c c a a c g a t c a g a t g g c g c t g g g c g c a a t g c g c g c c a t t a c c g g g t g a c g c t a c g a c c a a c g g t t g c t a g t c t a c c g c g a c c c g c g t t a c g c g c g g t a a t g g

7980 260 265 270 275 ESGLRVGADISVVGYDDTED lacI ESGLRVGADISVVGYDDTED g a g t c c g g g c t g c g c g t t g g t g c g g a c a t c t c g g t a g t g g g a t a c g a c g a t a c c g a a g a c c t c a g g c c c g a c g c g c a a c c a c g c c t g t a g a g c c a t c a c c c t a t g c t g c t a t g g c t t c t g

HpaI 8040 280 285 290 295 SSCYIPPLTTIKQDFRLLGQ lacI SSCYIPPLTTIKQDFRLLGQ a g c t c a t g t t a t a t c c c g c c g t t a a c c a c c a t c a a a c a g g a t t t t c g c c t g c t g g g g c a a t c g a g t a c a a t a t a g g g c g g c a a t t g g t g g t a g t t t g t c c t a a a a g c g g a c g a c c c c g t t

8100 300 305 310 315 TSVDRLLQLSQGQAVKGNQL lacI TSVDRLLQLSQGQAVKGNQL a c c a g c g t g g a c c g c t t g c t g c a a c t c t c t c a g g g c c a g g c g g t g a a g g g c a a t c a g c t g t g g t c g c a c c t g g c g a a c g a c g t t g a g a g a g t c c c g g t c c g c c a c t t c c c g t t a g t c g a c

KasINarI*SfoIPluTIBsmBIEsp3I 8160 320 325 330 335 LPVSLVKRKTTLAPNTQTAS lacI LPVSLVKRKTTLAPNTQTAS t t g c c c g t c t c a c t g g t g a a a a g a a a a a c c a c c c t g g c g c c c a a t a c g c a a a c c g c c t c t a a c g g g c a g a g t g a c c a c t t t t c t t t t t g g t g g g a c c g c g g g t t a t g c g t t t g g c g g a g a

8220 340 345 350 355 PRALADSLMQLARQVSRLES lacI PRALADSLMQLARQVSRLES c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g

8280 360 GQ* lacI GQ* g g g c a g t g a g c g c a a c g c a a t t a a t g t a a g t t a g c t c a c t c a t t a g g c a c c g g g a t c t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a t t c a a t c g a g t g a g t a a t c c g t g g c c c t a g a g c

3ʹ 5ʹ 8288 a c c g a t g c t g g c t a c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.