Addgene pCAG-GST-ATG101-Y45D Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCAG-GST-ATG101-Y45D Sequencing Result


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HincII (3) SalI (1) SspI (6103) Amp-R (5849 .. 5868) BsaI (5360) AhdI (5299) PspFI (4714) BseYI (4710) pBR322ori-F (4547 .. 4566) DrdI (4514) L4440 (4296 .. 4313) EBV-rev (4185 .. 4204) BsmI (4174) SV40 poly(A) signal SV40pA-R (4131 .. 4150) PsiI (4113) AvrII (4073) StuI (4072) SfiI (4026) BsaBI * (3892) CAP binding site M13/pUC Reverse (3733 .. 3755) lac operator M13 Reverse (3720 .. 3736) HindIII (3702) PstI (3697) PflMI (3443) rbglobpA-R (3358 .. 3377) Bglob-pA-R (3239 .. 3258) BstXI (3215) SmaI (3127) TspMI - XmaI (3125) SpeI (18) SnaBI (359) AscI (506) PspOMI (861) ApaI (865) EagI (1013) BlpI (1157) BsiWI (1189) PaqCI (1227) MreI - SgrAI (1315) pCAGGS-5 (1601 .. 1623) pCAG-F (1685 .. 1704) BspDI - ClaI (1746) EcoNI (1770) BstBI (2157) SwaI (2187) BclI * (2194) pGEX 5' (2371 .. 2393) Acc65I (2462) KpnI (2466) PpuMI * (2485) XcmI (2611) BsmBI - Esp3I (2736) PasI (2820) PmlI (2848) BbsI (2989) AgeI (3005) PshAI (3006) pCAG-GST-ATG101-Y45D 6218 bp

Sequence View

SpeISalIHincII 60 CMV enhancer g t c g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a c a g c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t

120 CMV enhancer g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g

180 CMV enhancer c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c

240 CMV enhancer g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g

300 CMV enhancer a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c

SnaBI 360 CMV enhancer c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c

420 CMV enhancer chicken β- actin promoter t a t t a g t c a t c g c t a t t a c c a t g g t c g a g g t g a g c c c c a c g t t c t g c t t c a c t c t c c c c a a t a a t c a g t a g c g a t a a t g g t a c c a g c t c c a c t c g g g g t g c a a g a c g a a g t g a g a g g g g t

480 chicken β- actin promoter t c t c c c c c c c c t c c c c a c c c c c a a t t t t g t a t t t a t t t a t t t t t t a a t t a t t t t g t g c a g a g a g g g g g g g g a g g g g t g g g g g t t a a a a c a t a a a t a a a t a a a a a a t t a a t a a a a c a c g t c

AscI 540 chicken β- actin promoter c g a t g g g g g c g g g g g g g g g g g g g g g g c g c g c c r g g s g g g g s g g g g s g g g g s g r g g g g s g g g c t a c c c c c g c c c c c c c c c c c c c c c c g c g c g g y c c s c c c c s c c c c s c c c c s c y c c c c s c c

600 chicken β- actin promoter g g s g g g g s g a g g c g g a g a g g t g c g g c g g c a g c c a a t c a g a g c g g c g c g c t c c g a a a g t t t c c s c c c c s c t c c g c c t c t c c a c g c c g c c g t c g g t t a g t c t c g c c g c g c g a g g c t t t c a a a

660 chicken β- actin promoter c c t t t t a t g g c g a g g c g g c g g c g g c g g c g g c c c t a t a a a a a g c g a a g c g c g c g g c g g g c g g g a a a a t a c c g c t c c g c c g c c g c c g c c g c c g g g a t a t t t t t c g c t t c g c g c g c c g c c c g c

720 chimeric intron g g a g t c g c t g c g c g c t g c c t t c g c c c c g t g c c c c g c t c c g c c g c c g c c t c g c g c c g c c c g c c t c a g c g a c g c g c g a c g g a a g c g g g g c a c g g g g c g a g g c g g c g g c g g a g c g c g g c g g g c

780 chimeric intron *EWLHAPPVARRR c c c c g g c t c t g a c t g a c c g c g t t a c t c c c a c a g g t g a g c g g g c g g g a c g g c c c t t c t c c t g g g g c c g a g a c t g a c t g g c g c a a t g a g g g t g t c c a c t c g c c c g c c c t g c c g g g a a g a g g a

840 chimeric intron RATILAQNLSPKNRKQPQTF c c g g g c t g t a a t t a g c g c t t g g t t t a a t g a c g g c t t g t t t c t t t t c t g t g g c t g c g t g a a g g c c c g a c a t t a a t c g c g a a c c a a a t t a c t g c c g a a c a a a g a a a a g a c a c c g a c g c a c t t

PspOMIApaI 900 chimeric intron AKLPEPLARQAPPAARPTRA a g c c t t g a g g g g c t c c g g g a g g g c c c t t t g t g c g g g g g g a g c g g c t c g g g g g g t g c g t g c t c g g a a c t c c c c g a g g c c c t c c c g g g a a a c a c g c c c c c c t c g c c g a g c c c c c c a c g c a c g

960 chimeric intron HTHAHPAGRAAGRQGAATLA g t g t g t g t g t g c g t g g g g a g c g c c g c g t g c g g c t c c g c g c t g c c c g g c g g c t g t g a g c g c c a c a c a c a c a c g c a c c c c t c g c g g c g c a c g c c g a g g c g c g a c g g g c c g c c g a c a c t c g c g

EagI 1020 chimeric intron APAARPKTREATHALPARGP t g c g g g c g c g g c g c g g g g c t t t g t g c g c t c c g c a g t g t g c g c g a g g g g a g c g c g g c c g g g a c g c c c g c g c c g c g c c c c g a a a c a c g c g a g g c g t c a c a c g c g c t c c c c t c g c g c c g g c c c

1080 chimeric intron ATGRPAPPAALPVFAAHPTH g g c g g t g c c c c g c g g t g c g g g g g g g g c t g c g a g g g g a a c a a a g g c t g c g t g c g g g g t g t g c c g c c a c g g g g c g c c a c g c c c c c c c c g a c g c t c c c c t t g t t t c c g a c g c a c g c c c c a c a c

1140 chimeric intron AHPPSCPTHARRDPQLGGQV t g c g t g g g g g g g t g a g c a g g g g g t g t g g g c g c g t c g g t c g g g c t g c a a c c c c c c c t g c a c a c g c a c c c c c c c a c t c g t c c c c c a c a c c c g c g c a g c c a g c c c g a c g t t g g g g g g g a c g t g

BsiWIBlpI 1200 chimeric intron GRGSNSLVARSRTRPETRPT c c c c c t c c c c g a g t t g c t g a g c a c g g c c c g g c t t c g g g t g c g g g g c t c c g t a c g g g g c g t g g g g g a g g g g c t c a a c g a c t c g t g c c g g g c c g a a g c c c a c g c c c c g a g g c a t g c c c c g c a

PaqCI 1260 chimeric intron ARPEGHRAPPPPLHPHRAPR g g c g c g g g g c t c g c c g t g c c g g g c g g g g g g t g g c g g c a g g t g g g g g t g c c g g g c g g g g c g c c g c g c c c c g a g c g g c a c g g c c c g c c c c c c a c c g c c g t c c a c c c c c a c g g c c c g c c c c g c

SgrAIMreI 1320 chimeric intron PRRPGPXARPLPAAAGPAGA g g g c c g c c t c g g g c c g g g g r g g g c t c g g g g g a g g g g c g c g g c g g c c c c c g g a g c g c c g g c c c c g g c g g a g c c c g g c c c c y c c c g a g c c c c c t c c c c g c g c c g c c g g g g g c c t c g c g g c c g

1380 chimeric intron ATSARRAAAM g g c t g t c g a g g c g c g g c g a g c c g c a g c c a t t g c c t t t t a t g g t a a t c g t g c g a g a g g g c g c c g a c a g c t c c g c g c c g c t c g g c g t c g g t a a c g g a a a a t a c c a t t a g c a c g c t c t c c c g c

1440 chimeric intron c a g g g a c t t c c t t t g t c c c a a a t c t g t g c g g a g c c g a a a t c t g g g a g g c g c c g c c g c a c c g t c c c t g a a g g a a a c a g g g t t t a g a c a c g c c t c g g c t t t a g a c c c t c c g c g g c g g c g t g g

1500 chimeric intron c c c t c t a g c g g g c g c g g g g c g a a g c g g t g c g g c g c c g g c a g g a a g g a a a t g g g c g g g g a g g g g a g a t c g c c c g c g c c c c g c t t c g c c a c g c c g c g g c c g t c c t t c c t t t a c c c g c c c c t c

1560 chimeric intron g g c c t t c g t g c g t c g c c g c g c c g c c g t c c c c t t c t c c c t c t c c a g c c t c g g g g c t g t c c g c c g g a a g c a c g c a g c g g c g c g g c g g c a g g g g a a g a g g g a g a g g t c g g a g c c c c g a c a g g c

1620 chimeric intron G G T T C G G C T T C T G G C G T G T G pCAGGS-5 c g g g g g g a c g g c t g c c t t c g g g g g g g a c g g g g c a g g g c g g g g t t c g g c t t c t g g c g t g t g g c c c c c c t g c c g a c g g a a g c c c c c c c t g c c c c g t c c c g c c c c a a g c c g a a g a c c g c a c a c

1680 chimeric intron A C C pCAGGS-5 MFMPSSFSYSS a c c g g c g g c t c t a g a g c c t c t g c t a a c c a t g t t c a t g c c t t c t t c t t t t t c c t a c a g c t c t g g c c g c c g a g a t c t c g g a g a c g a t t g g t a c a a g t a c g g a a g a a g a a a a a g g a t g t c g a g

1740 G C A A C G T G C T G G T T A T T G T G pCAG-F WATCWLLCCLIILAKNSSID c t g g g c a a c g t g c t g g t t a t t g t g c t g t c t c a t c a t t t t g g c a a a g a a t t c c t c g a t c g a g a c c c g t t g c a c g a c c a a t a a c a c g a c a g a g t a g t a a a a c c g t t t c t t a a g g a g c t a g c t

EcoNIBspDIClaI 1800 1 5 10 15 MASPILGYWKIKGLV GST Kozak sequence GIDATMASPILGYWKIKGLV c g g t a t c g a t g c c a c c a t g g c a t c c c c t a t a c t a g g t t a t t g g a a a a t t a a g g g c c t t g t g c c a t a g c t a c g g t g g t a c c g t a g g g g a t a t g a t c c a a t a a c c t t t t a a t t c c c g g a a c a

1860 20 25 30 35 QPTRLLLEYLEEKYEEHLYE GST QPTRLLLEYLEEKYEEHLYE g c a a c c c a c t c g a c t t c t t t t g g a a t a t c t t g a a g a a a a a t a t g a a g a g c a t t t g t a t g a c g t t g g g t g a g c t g a a g a a a a c c t t a t a g a a c t t c t t t t t a t a c t t c t c g t a a a c a t a c t

1920 40 45 50 55 RDEGDKWRNKKFELGLEFPN GST RDEGDKWRNKKFELGLEFPN g c g c g a t g a a g g t g a t a a a t g g c g a a a c a a a a a g t t t g a a t t g g g t t t g g a g t t t c c c a a c g c g c t a c t t c c a c t a t t t a c c g c t t t g t t t t t c a a a c t t a a c c c a a a c c t c a a a g g g t t

1980 60 65 70 75 LPYYIDGDVKLTQSMAIIRY GST LPYYIDGDVKLTQSMAIIRY t c t t c c t t a t t a t a t t g a t g g t g a t g t t a a a t t a a c a c a g t c t a t g g c c a t c a t a c g t t a a g a a g g a a t a a t a t a a c t a c c a c t a c a a t t t a a t t g t g t c a g a t a c c g g t a g t a t g c a a t

2040 80 85 90 95 IADKHNMLGGCPKERAEISM GST IADKHNMLGGCPKERAEISM t a t a g c t g a c a a g c a c a a c a t g t t g g g t g g t t g t c c a a a a g a g c g t g c a g a g a t t t c a a t a t a t c g a c t g t t c g t g t t g t a c a a c c c a c c a a c a g g t t t t c t c g c a c g t c t c t a a a g t t a

2100 100 105 110 115 LEGAVLDIRYGVSRIAYSKD GST LEGAVLDIRYGVSRIAYSKD g c t t g a a g g a g c g g t t t t g g a t a t t a g a t a c g g t g t t t c g a g a a t t g c a t a t a g t a a a g a c g a a c t t c c t c g c c a a a a c c t a t a a t c t a t g c c a c a a a g c t c t t a a c g t a t a t c a t t t c t

BstBI 2160 120 125 130 135 FETLKVDFLSKLPEMLKMFE GST FETLKVDFLSKLPEMLKMFE c t t t g a a a c t c t c a a a g t t g a t t t t c t t a g c a a g c t a c c t g a a a t g c t g a a a a t g t t c g a g a a a c t t t g a g a g t t t c a a c t a a a a g a a t c g t t c g a t g g a c t t t a c g a c t t t t a c a a g c t

BclI*SwaI 2220 140 145 150 155 DRLCHKTYLNGDHVTHPDFM GST DRLCHKTYLNGDHVTHPDFM a g a t c g t t t a t g t c a t a a a a c a t a t t t a a a t g g t g a t c a t g t a a c c c a t c c t g a c t t c a t t c t a g c a a a t a c a g t a t t t t g t a t a a a t t t a c c a c t a g t a c a t t g g g t a g g a c t g a a g t a

2280 160 165 170 175 LYDALDVVLYMDPMCLDAFP GST LYDALDVVLYMDPMCLDAFP g t t g t a t g a c g c t c t t g a t g t t g t t t t a t a c a t g g a c c c a a t g t g c c t g g a t g c g t t c c c c a a c a t a c t g c g a g a a c t a c a a c a a a a t a t g t a c c t g g g t t a c a c g g a c c t a c g c a a g g g

2340 180 185 190 195 KLVCFKKRIEAIPQIDKYLK GST KLVCFKKRIEAIPQIDKYLK a a a a t t a g t t t g t t t t a a a a a a c g t a t t g a a g c t a t c c c a c a a a t t g a t a a g t a c t t g a a t t t t a a t c a a a c a a a a t t t t t t g c a t a a c t t c g a t a g g g t g t t t a a c t a t t c a t g a a c t t

2400 200 205 210 215 SSKYIAWPLQGWQATFGGGD GST G G G C T G G C A A G C C A C G T T T G G T G pGEX 5' SSKYIAWPLQGWQATFGGGD a t c c a g c a a g t a t a t a g c a t g g c c t t t g c a g g g c t g g c a a g c c a c g t t t g g t g g t g g c g a t a g g t c g t t c a t a t a t c g t a c c g g a a a c g t c c c g a c c g t t c g g t g c a a a c c a c c a c c g c t

2460 HPPK (in frame with GST) SDDYDIPTT 1 5 ENLYFQG GST TEV site HPPKSDDYDIPTTENLYFQG c c a t c c t c c a a a a t c g g a t g a t t a c g a t a t c c c a a c g a c c g a a a a c c t g t a t t t t c a g g g g g t a g g a g g t t t t a g c c t a c t a a t g c t a t a g g g t t g c t g g c t t t t g g a c a t a a a a g t c c c

PpuMI*Acc65IKpnI 2520 (in frame with TEV site) GTMNCRSEVLEVSVEGRQVE TEV site GTMNCRSEVLEVSVEGRQVE t g g t a c c a t g a a t t g c c g g t c a g a g g t c c t g g a a g t g a g c g t g g a a g g t a g a c a g g t g g a a c c a t g g t a c t t a a c g g c c a g t c t c c a g g a c c t t c a c t c g c a c c t t c c a t c t g t c c a c c t

2580 (in frame with TEV site) EAMLAVLHTVLLHRSTGKFH EAMLAVLHTVLLHRSTGKFH a g a g g c t a t g c t g g c c g t g c t g c a t a c a g t g c t g c t g c a c a g a t c a a c t g g c a a g t t c c a t c t c c g a t a c g a c c g g c a c g a c g t a t g t c a c g a c g a c g t g t c t a g t t g a c c g t t c a a g g t

XcmI 2640 (in frame with TEV site) YKKEGTDSIGTVGTQDVDCD YKKEGTDSIGTVGTQDVDCD t t a c a a g a a a g a a g g a a c a g a t t c c a t c g g a a c c g t g g g t a c t c a g g a c g t c g a t t g c g a a a t g t t c t t t c t t c c t t g t c t a a g g t a g c c t t g g c a c c c a t g a g t c c t g c a g c t a a c g c t

2700 (in frame with TEV site) FIDFTYVRVSSEELDRALRK FIDFTYVRVSSEELDRALRK c t t c a t t g a t t t t a c t t a c g t g c g c g t c a g c t c c g a g g a a c t g g a c a g a g c a c t c c g t a a g a a g t a a c t a a a a t g a a t g c a c g c g c a g t c g a g g c t c c t t g a c c t g t c t c g t g a g g c a t t

BsmBIEsp3I 2760 (in frame with TEV site) VVGEFKDALRNSGGDGLGQM VVGEFKDALRNSGGDGLGQM g g t g g t c g g a g a a t t c a a a g a t g c c c t g a g g a a c a g t g g c g g a g a c g g t c t g g g c c a g a t c c a c c a g c c t c t t a a g t t t c t a c g g g a c t c c t t g t c a c c g c c t c t g c c a g a c c c g g t c t a

2820 (in frame with TEV site) SLEFYQKKKSRWPFSDECIP SLEFYQKKKSRWPFSDECIP g a g c c t c g a g t t c t a t c a g a a g a a a a a g t c t a g a t g g c c a t t t t c a g a t g a a t g t a t c c c c t c g g a g c t c a a g a t a g t c t t c t t t t t c a g a t c t a c c g g t a a a a g t c t a c t t a c a t a g g g

PmlIPasI 2880 (in frame with TEV site) WEVWTVKVHVVALATEQERQ WEVWTVKVHVVALATEQERQ c t g g g a a g t g t g g a c c g t g a a g g t c c a c g t g g t c g c c c t g g c t a c t g a g c a g g a a c g g c a g a c c c t t c a c a c c t g g c a c t t c c a g g t g c a c c a g c g g g a c c g a t g a c t c g t c c t t g c c g t

2940 (in frame with TEV site) ICREKVGEKLCEKIINIVEV ICREKVGEKLCEKIINIVEV g a t c t g c c g c g a g a a a g t g g g c g a a a a g c t c t g t g a g a a g a t c a t c a a c a t c g t g g a a g t c t a g a c g g c g c t c t t t c a c c c g c t t t t c g a g a c a c t c t t c t a g t a g t t g t a g c a c c t t c a

BbsI 3000 (in frame with TEV site) MNRHEYLPKMPTQSEVDNVF MNRHEYLPKMPTQSEVDNVF c a t g a a c c g t c a t g a g t a c c t g c c c a a g a t g c c t a c a c a g a g c g a g g t g g a t a a t g t c t t g t a c t t g g c a g t a c t c a t g g a c g g g t t c t a c g g a t g t g t c t c g c t c c a c c t a t t a c a g a a

AgeIPshAI 3060 (in frame with TEV site) DTGLRDVQPYLYKISFQITD DTGLRDVQPYLYKISFQITD c g a c a c c g g t c t g c g g g a t g t g c a g c c c t a c c t c t a t a a g a t c t c c t t t c a g a t t a c a g a g c t g t g g c c a g a c g c c c t a c a c g t c g g g a t g g a g a t a t t c t a g a g g a a a g t c t a a t g t c t

3120 (in frame with TEV site) ALGTSVTTTMRRLIKDTLAL ALGTSVTTTMRRLIKDTLAL c g c t c t g g g c a c c t c t g t c a c c a c t a c a a t g c g c a g g c t c a t c a a a g a c a c c c t g g c a c t g c g a g a c c c g t g g a g a c a g t g g t g a t g t t a c g c g t c c g a g t a g t t t c t g t g g g a c c g t g a

TspMIXmaISmaI 3180 ** c t a a c c c g g g t g a t a t c t t a a t t a a t t a a c t c g a g g a a t t c a c t c c t c a g g t g c a g g c t g g a t t g g g c c c a c t a t a g a a t t a a t t a a t t g a g c t c c t t a a g t g a g g a g t c c a c g t c c g a c

BstXI 3240 A G Bglob-pA-R c c t a t c a g a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a g a t c g g a t a g t c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t c t a g

3300 A A A A A G G G A G A C G G T T T T Bglob-pA-R t t t t t c c c t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t t c t g a a a a a g g g a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a a g a c

3360 β- globin poly(A) signal G T G rbglobpA-R g c t a a t a a a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c c g a t t a t t t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g

3420 A G C C T T C C T G T A T A C C C rbglobpA-R t c g g a a g g a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t t a g a a g c c t t c c t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a a t c t

PflMI 3480 g t t t g g c a a c a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a g g t c c a a a c c g t t g t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t c c a g

3540 a t c a g t a t a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g a c t t t a g t c a t a t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c t g a a

3600 g a g g t t a g a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a a a a t c t c c a a t c t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t t t t a

3660 t t t c c t t a c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c a t a g a a a g g a a t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g t a t c

HindIIIPstI 3720 M13 rev C M13 Reverse c t g t c c c t c t t c t c t t a t g g a g a t c c c t c g a c c t g c a g c c c a a g c t t g g c g t a a t c a t g g g a c a g g g a g a a g a g a a t a c c t c t a g g g a g c t g g a c g t c g g g t t c g a a c c g c a t t a g t a c c

3780 -10 M13 rev lac operator lac promoter A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g

3840 -35 lac promoter CAP binding site g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c

BsaBI* 3900 SV40 promoter t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c g g a t c c g c a t c t c a a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g c c t a g g c g t a g a g t

3960 SV40 promoter SV40 ori a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t

4020 SV40 promoter SV40 ori g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c

StuIAvrIISfiI 4080 SV40 promoter SV40 ori c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a

PsiI 4140 SV40 promoter SV40 poly(A) signal C G T T A T C G T A SV40pA-R t t t g c a a a a a g c t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t a a a c g t t t t t c g a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a

BsmI 4200 SV40 poly(A) signal G T G G T T T G T C C A A A C T EBV-rev G T G T T T A A A G SV40pA-R c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a

4260 SV40 poly(A) signal C A T C EBV-rev c a t c a a t g t a t c t t a t c a t g t c t g g a t c c g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g g t a g t t a c a t a g a a t a g t a c a g a c c t a g g c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c

4320 G A G C G A G T G A C T G A G C G A L4440 a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c

4380 g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t

4440 g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g

4500 ori c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g

DrdI 4560 ori T T T C T A T G G T C C G C pBR322ori-F a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c

4620 ori A A A G G G pBR322ori-F t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g

4680 ori c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a

BseYIPspFI 4740 ori c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c

4800 ori c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g

4860 ori t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a

4920 ori g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a

4980 ori a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g

5040 ori a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t

5100 ori a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g

5160 g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g

5220 c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a

5280 285 280 275 270 *WHKILSAGIEAIQRNRE AmpR *WHKILSAGIEAIQRNRE g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t

AhdI 5340 265 260 255 250 DMTAQSGTTYIVVIRSPKGD AmpR DMTAQSGTTYIVVIRSPKGD t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a

BsaI 5400 245 240 235 230 PGLAAIIGRSGREGAGSKDA AmpR PGLAAIIGRSGREGAGSKDA g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t

5460 225 220 215 210 IFWGAPLASRLLPGAVKDAE AmpR IFWGAPLASRLLPGAVKDAE a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g

5520 205 200 195 190 MWDILQQRSALTLLEGTLLK AmpR MWDILQQRSALTLLEGTLLK a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c

5580 185 180 175 170 RLTTAMAVPMTTDREDNPIA AmpR RLTTAMAVPMTTDREDNPIA c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a

5640 165 160 155 150 ENLEPEWRDLRTVHDGMNHL AmpR ENLEPEWRDLRTVHDGMNHL t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t

5700 145 140 135 130 FATLEKPGGITTLLLNAATN AmpR FATLEKPGGITTLLLNAATN a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t

5760 125 120 115 110 DSMTIAASCLERVTMGDTLH AmpR DSMTIAASCLERVTMGDTLH t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g

5820 105 100 95 90 KETVPSYEVLDNQSYHIRRG AmpR KETVPSYEVLDNQSYHIRRG t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c

5880 85 80 75 70 LQEQGADIRSLVAGCLLVKF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R LQEQGADIRSLVAGCLLVKF a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t

5940 65 60 55 50 TSMMPFREEPRFSELIKGSN AmpR TSMMPFREEPRFSELIKGSN g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c

6000 45 40 35 30 LDLEIYGVRAGLQDEADKVK AmpR LDLEIYGVRAGLQDEADKVK a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g

6060 25 VLTEPH 20 15 10 signal sequence AFVPLCFAAFFPIL AmpR VLTEPHAFVPLCFAAFFPIL a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c

SspI 6120 5 1 signal sequence AVRFHQISM AmpR AmpR promoter AVRFHQISM g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a

6180 AmpR promoter c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t

6218 AmpR promoter g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g g c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.