Addgene pD649-HAsp-CEACAM3-Fc(DAPA)-AviTag-6xHis Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pD649-HAsp-CEACAM3-Fc(DAPA)-AviTag-6xHis Sequencing Result


Map View

EarI (52) Puro-F (6168 .. 6188) DraIII (6115) SV40pA-R (5899 .. 5918) BsmI (5876) EBV-rev (5845 .. 5864) SalI (5813) AfeI (5657) FspI (5485) PvuI (5339) ScaI (5227) Amp-R (5139 .. 5158) Kan-R (4768 .. 4787) NruI (4783) pENTR-R (4743 .. 4762) MauBI (4706) pBR322ori-F (4149 .. 4168) L4440 (3898 .. 3915) M13 Forward (3783 .. 3800) AflII (3777) ApaI (3772) PspOMI (3768) RsrII (95) BsiWI (154) Puro-R (196 .. 215) TspMI - XmaI (318) SmaI (320) AvrII (339) SV40pro-F (397 .. 416) SV40 promoter pBABE 3' (643 .. 663) BGH-rev (881 .. 898) MluI (1005) FseI (1032) 6xHis XcmI (1467) PaqCI (1758) AscI (1793) BbvCI (2085) NotI (2165) NheI (2226) BmtI (2230) pMT2-F (2280 .. 2299) SP6 (2313 .. 2330) BstXI (2393) Eco53kI (2562) SacI (2564) LNCX (2540 .. 2564) CMV-F (2590 .. 2610) SnaBI (2788) NdeI (2892) SpeI (3125) pD649-HAsp-CEACAM3-Fc(DAPA)-AviTag-6xHis 6469 bp

Sequence View

EarI 5Ęą 3Ęą 60 70 65 60 55 AAGDDAVWVKGIDLGVRTLF PuroR AGDDAVWVKGIDLGVRTLF g c g g c g c c g t c g t c c g c g a c c c a c a c c t t g c c g a t g t c g a g c c c g a c g c g c g t g a g g a a g c g c c g c g g c a g c a g g c g c t g g g t g t g g a a c g g c t a c a g c t c g g g c t g c g c g c a c t c c t t c

RsrII 120 50 45 40 35 LEQLETVREIHRDPDVTHRT PuroR LEQLETVREIHRDPDVTHRT a g t t c t t g c a g c t c g g t g a c c c g c t c g a t g t g g c g g t c c g g a t c g a c g g t g t g g c g c g t g t c a a g a a c g t c g a g c c a c t g g g c g a g c t a c a c c g c c a g g c c t a g c t g c c a c a c c g c g c a c

BsiWI 180 30 25 20 15 APYDAFAAALTRVARPVDDR PuroR APYDAFAAALTRVARPVDDR g c g g g g t a g t c g g c g a a c g c g g c g g c g a g g g t g c g t a c g g c c c t g g g g a c g t c g t c g c g g c g c c c c a t c a g c c g c t t g c g c c g c c g c t c c c a c g c a t g c c g g g a c c c c t g c a g c a g c g c c

240 10 5 1 TALRVTPKYETM PuroR EM7 promoter G T G G G C T T G T A C T C G G T C A T Puro-R TALRVTPKYETM g t g g c g a g g c g c a c c g t g g g c t t g t a c t c g g t c a t g g t t t a g t t c c t c a c c t t g t c g t a t c a c c g c t c c g c g t g g c a c c c g a a c a t g a g c c a g t a c c a a a t c a a g g a g t g g a a c a g c a t a

300 EM7 promoter t a t a c t a t g c c g a t a t a c t a t g c c g a t g a t t a a t t g t c a a c a c g t g c t g a t c a g a t c c g a a t a t g a t a c g g c t a t a t g a t a c g g c t a c t a a t t a a c a g t t g t g c a c g a c t a g t c t a g g c t

AvrIITspMIXmaISmaI 360 SV40 promoter SV40 ori a a a t g g a t a t a c a a g c t c c c g g g a g c t t t t t g c a a a a g c c t a g g c c t c c a a a a a a g c c t c t t t a c c t a t a t g t t c g a g g g c c c t c g a a a a a c g t t t t c g g a t c c g g a g g t t t t t t c g g a g

420 SV40 promoter SV40 ori G G A G C C G G A G A C G T A T T T A T SV40pro-F c t c a c t a c t t c t g g a a t a g c t c a g a g g c a g a g g c g g c c t c g g c c t c t g c a t a a a t a a a a a g a g t g a t g a a g a c c t t a t c g a g t c t c c g t c t c c g c c g g a g c c g g a g a c g t a t t t a t t t t t

480 SV40 promoter SV40 ori a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g c g g a a c t g g g c g g a g t t a g g g g c g g g a t g g t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c g c c t t g a c c c g c c t c a a t c c c c g c c c t a c c

540 SV40 promoter g c g g a g t t a g g g g c g g g a c t a t g g t t g c t g a c t a a t t g a g a t g c a t g c t t t g c a t a c t t c c g c c t c a a t c c c c g c c c t g a t a c c a a c g a c t g a t t a a c t c t a c g t a c g a a a c g t a t g a a g

600 SV40 promoter t g c c t g c t g g g g a g c c t g g g g a c t t t c c a c a c c t g g t t g c t g a c t a a t t g a g a t g c a t g c a c g g a c g a c c c c t c g g a c c c c t g a a a g g t g t g g a c c a a c g a c t g a t t a a c t c t a c g t a c g

660 SV40 promoter A C C C T A A C T G A C A C A C A T pBABE 3' t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t t t c c a c a c c c t a a c t g a c a c a c a t a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a a a g g t g t g g g a t t g a c t g t g t g t a

720 T C C pBABE 3' t c c a c a g a a t t a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c a g g t g t c t t a a t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g

780 c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a

840 bGH poly(A) signal g a t g c a a t t t c c t c a t t t t a t t a g g a a a g g a c a g t g g g a g t g g c a c c t t c c a g g g t c a a g c t a c g t t a a a g g a g t a a a a t a a t c c t t t c c t g t c a c c c t c a c c g t g g a a g g t c c c a g t t c

900 bGH poly(A) signal T A G A A G G C A C A G T C G A G G BGH-rev g a a g g c a c g g g g g a g g g g c a a a c a a c a g a t g g c t g g c a a c t a g a a g g c a c a g t c g a g g c t c t t c c g t g c c c c c t c c c c g t t t g t t g t c t a c c g a c c g t t g a t c t t c c g t g t c a g c t c c g a

960 g a t t t t c t c t a t c t a t t t a t t t t a c t a c t c a a g a c t a t c t t g c a a a g t a g a a g a a c c c t g c t a a a a g a g a t a g a t a a a t a a a a t g a t g a g t t c t g a t a g a a c g t t t c a t c t t c t t g g g a c

MluI 1020 MTTVYTRLAYRVTRCAS t c t g c a g t a t g a c g a c c g t g t a t a c c c g a t t g g c t t a c c g g g t a a c g c g t t g t g c c a g t g a g a c g t c a t a c t g c t g g c a c a t a t g g g c t a a c c g a a t g g c c c a t t g c g c a a c a c g g t c a c

FseI 1080 * 5 1 HHHHHH 15 10 EHWEIKQA 6xHis AviTag™ DYRPALVVMVVVFMPLNLLS *HHHHHHEHWEIKQA a c t a c a g g c c g g c c t t a g t g g t g a t g g t g g t g g t g t t c a t g c c a c t c a a t c t t c t g a g c c t g a t g t c c g g c c g g a a t c a c c a c t a c c a c c a c c a c a a g t a c g g t g a g t t a g a a g a c t c g g

1140 5 1 EFIDNLG AviTag™ LENIIKTAAAGAQAQGLLGV EFIDNLGSGGPSLSLSKQTY t c g a a a a t a t c a t t a a g a c c g c t g c c g c c g g g g c t c a g g c t c a g g g a c t t c t g g g t g t a g a g c t t t t a t a g t a a t t c t g g c g a c g g c g g c c c c g a g t c c g a g t c c c t g a a g a c c c a c a t c

1200 VVVQGLVHHAAAEHVALLPP HNHLAEHMVSCSFVNGQQWR t g g t t g t g c a g g g c c t c g t g c a t c a c g c t g c a g c t g a a c a c g t t g c c c t g c t g c c a c c t g a c c a a c a c g t c c c g g a g c a c g t a g t g c g a c g t c g a c t t g t g c a a c g g g a c g a c g g t g g a c

1260 GLVHGQLAVQEEAAVAVQHW SKDVTLKSYLFFSGDSDLVP g a c t t g t c c a c g g t c a g c t t g c t g t a c a g g a a g a a g c t g c c g t c g c t g t c c a g c a c t g g g c t g a a c a g g t g c c a g t c g a a c g a c a t g t c c t t c t t c g a c g g c a g c g a c a g g t c g t g a c c c

1320 GGGLVVVLGLAVALPLHGDI PTTKYNNEPQGNSEWEVAID g g g g t g g t c t t g t a g t t g t t c t c g g g c t g g c c g t t g c t c t c c c a c t c c a c g g c g a t a t c g c c c c a c c a g a a c a t c a a c a a g a g c c c g a c c g g c a a c g a g a g g g t g a g g t g c c g c t a t a g c

1380 AGVEALHQTGQGHLVLGHLL SPYFGKVLCTLSVQNKTMEE c t g g g g t a g a a g c c c t t c a c c a g a c a g g t c a g g g a c a c c t g g t t c t t g g t c a t c t c c t c c g a c c c c a t c t t c g g g a a g t g g t c t g t c c a g t c c c t g t g g a c c a a g a a c c a g t a g a g g a g g

1440 PAGGQGVHLGLPWLALGLAD RSPPLTYVQPERPQGKAKSI c g g c t g g g g g g c a g g g t g t a c a c c t g g g g c t c c c g t g g c t g g c c c t t g g c c t t g c t g a t t g c c g a c c c c c c g t c c c a c a t g t g g a c c c c g a g g g c a c c g a c c g g g a a c c g g a a c g a c t a a

XcmI 1500 CLFDWGSQGLVGDFALVFFA TKEIPAALAKNSVKCKYEKG g t c t t t t c g a t t g g g g c a g c c a g g g c c t t g t t g g a g a c t t t g c a c t t g t a t t c t t t g c c g c a g a a a a g c t a a c c c c g t c g g t c c c g g a a c a a c c t c t g a a a c g t g a a c a t a a g a a a c g g c

1560 VQPVLVQHGQHGHHPVGAVV NLWDQHLVTLVSVVRYTSNY t t c a g c c a g t c c t g g t g c a g c a c g g t c a g c a c g g a c a c c a c c c t g t a g g t g c t g t t g t a c a a g t c g g t c a g g a c c a c g t c g t g c c a g t c g t g c c t g t g g t g g g a c a t c c a c g a c a a c a t g

1620 LLLSGLGLGVVHLHAVHVPV QEERPKTKANHVEVGDVYWN t g c t c c t c t c t g g g c t t g g t c t t g g c g t t g t g c a c c t c c a c g c c g t c c a c g t a c c a g t t g a c g a g g a g a g a c c c g a a c c a g a a c c g c a a c a c g t g g a g g t g c g g c a g g t g c a t g g t c a a c

1680 ELHLWVLVGHGHHHAGHLGG FKVEPDEHSVAVVVCTVEPT a a c t t c a c c t c t g g g t c c t c g t g g g a c a c g g c c a c c a c c a c g c a g g t c a c c t c g g g g g t c t t g a a g t g g a g a c c c a g g a g c a c c c t g t g c c g g t g g t g g t g c g t c c a g t g g a g c c c c c a g

1740 PADHQGVLGLGGEQEHGRPS RSIMLTDKPKPPFLFVSPGG c t g c t g a t c a t c a g g g t g t c c t t g g g c t t g g g g g g g a a c a g g a a c a c g g a a g g c c c t c c c g a c g a c t a g t a g t c c c a c a g g a a c c c g a a c c c c c c c t t g t c c t t g t g c c t t c c g g g a g g g

AscIPaqCI 1800 QQFWSWAGGAGVGLVAARRA LLEPAPCPPCTHTKDGGPAG a g c a g t t c t g g a g c t g g g c a g g g g g g g c a g g t g t g g g t c t t g t c g c c g c c c g g c g c g c c a t c g t c a a g a c c t c g a c c c g t c c c c c c c g t c c a c a c c c a g a a c a g c g g c g g g c c g c g c g g t

1860 TGNSSHRQSRGIFLVHVELA GAVAGVPLGPANEQYVHFQG c c g g c a a c a g c t c c c a c a g g c a g t c c a g g g g c a t t t t c t t g g t a c a c g t g g a a c t g g c c g g g c c g t t g t c g a g g g t g t c c g t c a g g t c c c c g t a a a a g a a c c a t g t g c a c c t t g a c c g g c

1920 GGFLIDQVALDHLQGVEADV TAEENVLDSKIVQLTYFGID g t g g c t t c c t c a t t g a c c a g g t c g c t c t t g a t c a c t t g c a g g g t g t a g a a g c c g a t g t c g c a c c g a a g g a g t a a c t g g t c c a g c g a g a a c t a g t g a a c g t c c c a c a t c t t c g g c t a c a g c

1980 VLGHVLDQQAGVGIDCLSAA NQTVNQILLSANTYITERGS t t c t g g g t c a c g t t c t g g a t c a g c a g g c t g g c g t t g g t a t a g a t t g t c t c t c t g c c g c t g a a g a c c c a g t g c a a g a c c t a g t c g t c c g a c c g c a a c c a t a t c t a a c a g a g a g a c g g c g a c

2040 VSGARCSLLCADHVAHDQAI YAAGPTAQQTGIVYGVILSN t a a g c g g c g c c a g g t g t a g c t t g t t g t g t g c c g a t c a c g t a g c c c a c g a t c a g g c t a t t g a t t c g c c g c g g t c c a c a t c g a a c a a c a c a c g g c t a g t g c a t c g g g t g c t a g t c c g a t a a c

BbvCI 2100 AVHSLALIPAVAEQVLRQVV GDVREGKYWSYGFLHQPLNH c c g t c c a c t c t c t c g c c c t t a t a c c a g c t g t a g c c g a a c a g g t g c t g a g g c a g g t t g t g a g g c a g g t g a g a g a g c g g g a a t a t g g t c g a c a t c g g c t t g t c c a c g a c t c c g t c c a a c a c t

2160 NQQQHLFSLGHAQRHRLDCQ VLLLVEKGEAVSLPMSEITL a c c a g c a g c a g c a c c t c t t t t c c c t c g g c c a c g c t c a g a g g c a t a g a c t c g a t t g t c a g c t g g t c g t c g t c g t g g a g a a a a g g g a g c c g g t g c g a g t c t c c g t a t c t g a g c t a a c a g t c g

NotI 2220 LGGRSEHQAEDVAQGNDGLH KAAAAFVLCFIYSLAIITKM t t g g c g g c c g c a g c g a a c a c c a g g c a g a a g a t g t a g c t c a g g g c a a t g a t g g t c t t c a t g a a c c g c c g g c g t c g c t t g t g g t c c g t c t t c t a c a t c g a g t c c c g t t a c t a c c a g a a g t a c

BmtINheI 2280 T pMT2-F GGASRRVFDRTEVQLDLGVD g t g g c g c t a g c a g g c g c g t t t t c g a t a g a a c c g a g g t g c a g t t g g a c c t g g g a g t g g a c a c a c c g c g a t c g t c c g c g c a a a a g c t a t c t t g g c t c c a c g t c a a c c t g g a c c c t c a c c t g t

2340 SP6 promoter G G A C A C C T C T C T T T C C G T T pMT2-F G A T A T C A C A G T G G A T T T A SP6 TCGEKGKVDVIL* c c t g t g g a g a g a a a g g c a a a g t g g a t g t t a t t c t a t a g t g t c a c c t a a a t c g t a t g t g t a g g a c a c c t c t c t t t c c g t t t c a c c t a c a a t a a g a t a t c a c a g t g g a t t t a g c a t a c a c a t

BstXI 2400 t g a t a c a t a a g g t t a t g t a t t a a t t g t a g c c g c g t t c t a a c g a a g c c a a g g g g g t g g g c c a c t a t g t a t t c c a a t a c a t a a t t a a c a t c g g c g c a a g a t t g c t t c g g t t c c c c c a c c c g g

2460 t a t a g a c t c t a t a g g c g g t a c t t a c g t c a c t c t t g g c a c g g g g a a t c c g c g t t c c a a t g c a t a t c t g a g a t a t c c g c c a t g a a t g c a g t g a g a a c c g t g c c c c t t a g g c g c a a g g t t a c g

2520 a c c g t t c c c g g c c g c g g a g g c t g g a t c g g t c c c g g t g t c t t c t a t g g a g g t c a a a a c a g c t g g c a a g g g c c g g c g c c t c c g a c c t a g c c a g g g c c a c a g a a g a t a c c t c c a g t t t t g t c g

Eco53kISacI 2580 CMV promoter C T A G A C T G C C A A G T G A T T T G C T C G A LNCX g t g g a t g g c g t c t c c a g g c g a t c t g a c g g t t c a c t a a a c g a g c t c t g c t t a t a t a g a c c t c a c c t a c c g c a g a g g t c c g c t a g a c t g c c a a g t g a t t t g c t c g a g a c g a a t a t a t c t g g a

2640 CMV promoter G T G C G G A T G G C G G G T A A A C G C CMV-F c c c a c c g t a c a c g c c t a c c g c c c a t t t g c g t c a a t g g g g c g g a g t t g t t a c g a c a t t t t g g g g t g g c a t g t g c g g a t g g c g g g t a a a c g c a g t t a c c c c g c c t c a a c a a t g c t g t a a a a c

2700 CMV promoter g a a a g t c c c g t t g a t t t t g g t g c c a a a a c a a a c t c c c a t t g a c g t c a a t g g g g t g g a g a c c t t t c a g g g c a a c t a a a a c c a c g g t t t t g t t t g a g g g t a a c t g c a g t t a c c c c a c c t c t g

2760 CMV promoter t t g g a a a t c c c c g t g a g t c a a a c c g c t a t c c a c g c c c a t t g a t g t a c t g c c a a a a c c g c a a a c c t t t a g g g g c a c t c a g t t t g g c g a t a g g t g c g g g t a a c t a c a t g a c g g t t t t g g c g t

SnaBI 2820 CMV promoter CMV enhancer t c a c c a t g g t a a t a g c g a t g a c t a a t a c g t a g a t g t a c t g c c a a g t a g g a a a g t c c c a t a a g t g g t a c c a t t a t c g c t a c t g a t t a t g c a t c t a c a t g a c g g t t c a t c c t t t c a g g g t a t

2880 CMV enhancer a g g t c a t g t a c t g g g c a t a a t g c c a g g c g g g c c a t t t a c c g t c a t t g a c g t c a a t a g g g g t c c a g t a c a t g a c c c g t a t t a c g g t c c g c c c g g t a a a t g g c a g t a a c t g c a g t t a t c c c c

NdeI 2940 CMV enhancer g c g t a c t t g g c a t a t g a t a c a c t t g a t g t a c t g c c a a g t g g g c a g t t t a c c g t a a a t a c t c g c a t g a a c c g t a t a c t a t g t g a a c t a c a t g a c g g t t c a c c c g t c a a a t g g c a t t t a t g a

3000 CMV enhancer c c a c c c a t t g a c g t c a a t g g a a a g t c c c t a t t g g c g t t a c t a t g g g a a c a t a c g t c a t t a g g t g g g t a a c t g c a g t t a c c t t t c a g g g a t a a c c g c a a t g a t a c c c t t g t a t g c a g t a a t

3060 CMV enhancer t t g a c g t c a a t g g g c g g g g g t c g t t g g g c g g t c a g c c a g g c g g g c c a t t t a c c g t a a g t t a a c t g c a g t t a c c c g c c c c c a g c a a c c c g c c a g t c g g t c c g c c c g g t a a a t g g c a t t c a a

3120 CMV enhancer a t g t a a c g c g g a a c t c c a t a t a t g g g c t a t g a a c t a a t g a c c c c g t a a t t g a t t a c t a t t t a c a t t g c g c c t t g a g g t a t a t a c c c g a t a c t t g a t t a c t g g g g c a t t a a c t a a t g a t a a

SpeI 3180 CMV enhancer a a t a a c t a g t c a a t a a t c a a t g c c a a c a t g g c g g t c a t a t t g g a c a t g a g c c a a t a t a a a t t a t t g a t c a g t t a t t a g t t a c g g t t g t a c c g c c a g t a t a a c c t g t a c t c g g t t a t a t t t

3240 t g t a c a t a t t a t g a t a t a g a t a c a a c g t a t g c a a t g g c c a a t a g c c a a t a t t g a t t t a t g a c a t g t a t a a t a c t a t a t c t a t g t t g c a t a c g t t a c c g g t t a t c g g t t a t a a c t a a a t a c

3300 c t a t a t a a c c a a t g a a t a a t a t g g c t a a t g g c c a a t a t t g a g t c a a c g c c a c t g t g c a t g g a t a t a t t g g t t a c t t a t t a t a c c g a t t a c c g g t t a t a a c t c a g t t g c g g t g a c a c g t a c

3360 lac operator a a g c t c g t a a t t g t t a t c c g c t c a c a a t t a a g g g c g a c a c a a a a t t t a t t c t a a a t g a t a t t c g a g c a t t a a c a a t a g g c g a g t g t t a a t t c c c g c t g t g t t t t a a a t a a g a t t t a c t a t

3420 a t a a a t a c t g a t a a c a t c t t a t a g t t t g t a t t a t a t t t t g t a t t a t c g t t g a c a t g t a t a t a t t t a t g a c t a t t g t a g a a t a t c a a a c a t a a t a t a a a a c a t a a t a g c a a c t g t a c a t a t

3480 a t t t t g a t a t c a a a a a c t g a t t t t c c c t t t a t t a t t t t c g a g a t t t a t t t t c t t a a t t c t t a a a a c t a t a g t t t t t g a c t a a a a g g g a a a t a a t a a a a g c t c t a a a t a a a a g a a t t a a g a

3540 c t t t a a c a a a c t a g a a a t a t t g t a t a t a c a a a a a a t c a t a a a t a a t a g a t g a a t a g t t t a g a a a t t g t t t g a t c t t t a t a a c a t a t a t g t t t t t t a g t a t t t a t t a t c t a c t t a t c a a a t

3600 a t t a t a g g t g t t c a t c a a t c g a a a a a g c a a c g t a t c t t a t t t a a a g t g c g t t g c t t t t t t t a a t a t c c a c a a g t a g t t a g c t t t t t c g t t g c a t a g a a t a a a t t t c a c g c a a c g a a a a a a

3660 c t c a t t t a t a a g g t t a a a t a a t t c t c a t a t a t c a a g c a a a g t g a c a g g c g c c c t t a a a t a g a g t a a a t a t t c c a a t t t a t t a a g a g t a t a t a g t t c g t t t c a c t g t c c g c g g g a a t t t a t

3720 t t c t g a c a a a t g c t c t t t c c c t a a a c t c c c c c c a t a a a a a a a c c c g c c g a a g c g g g t t t t a a g a c t g t t t a c g a g a a a g g g a t t t g a g g g g g g t a t t t t t t t g g g c g g c t t c g c c c a a a a

AflIIPspOMIApaI 3780 t a c g t t a t t t g c g g a t t a a c g a t t a c t c g t t a t c a g a a c c g c c c a g g g g g c c c g a g c t t a a t g c a a t a a a c g c c t a a t t g c t a a t g a g c a a t a g t c t t g g c g g g t c c c c c g g g c t c g a a t

3840 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward a g a c t g g c c g t c g t t t t a c a a c a c a g a a a g a g t t t g t a g a a a c g c a a a a a g g c c a t c c g t t c t g a c c g g c a g c a a a a t g t t g t g t c t t t c t c a a a c a t c t t t g c g t t t t t c c g g t a g g c a

3900 G A G L4440 c a g g g g c c t t c t g c t t a g t t t g a t g c c t g g c a g t t c c c t a c t c t c g c c t t c c g c t t c c t c g t c c c c g g a a g a c g a a t c a a a c t a c g g a c c g t c a a g g g a t g a g a g c g g a a g g c g a a g g a g

3960 C G A G T G A C T G A G C G A L4440 g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t

4020 g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t

4080 ori a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a

4140 ori c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g

4200 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g

4260 ori g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g

4320 ori t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c

4380 ori t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t

4440 ori g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c

4500 ori c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g g c t a a c t a c g g c t a g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c c g a t t g a t g c c g a t

4560 ori c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c

4620 ori a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c

4680 ori c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g

MauBI 4740 g g g g t c t g a c g c t c a g t g g a a c g a c g c g c g c g t a a c t c a c g t t a a g g g a t t t t g g t c a t g c c c c a g a c t g c g a g t c a c c t t g c t g c g c g c g c a t t g a g t g c a a t t c c c t a a a a c c a g t a c

NruI 4800 G T T C C C C A C A A T A C T C G G T A pENTR-R C C A T A T T T A C C C G A G C G C T A Kan-R a g c a a g g g g t g t t a t g a g c c a t a t t c a g g t a t a a a t g g g c t c g c g a t a a t g t t c a g a a t t t c g t t c c c c a c a a t a c t c g g t a t a a g t c c a t a t t t a c c c g a g c g c t a t t a c a a g t c t t a a

4860 AmpR promoter g g t t a a t t g g t t g t a a c a c t g a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g c c a a t t a a c c a a c a t t g t g a c t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c

4920 AmpR promoter t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a a t a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t t a

4980 1 5 10 15 20 signal sequence MSIQHFRVALIPFFAAFCLP AmpR MSIQHFRVALIPFFAAFCLP a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a

5040 signal sequence VFA 25 30 35 40 HPETLVKVKDAEDQLGA AmpR VFAHPETLVKVKDAEDQLGA g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t

5100 45 50 55 60 RVGYIELDLNSGKILESFRP AmpR RVGYIELDLNSGKILESFRP c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g

5160 65 70 75 80 EERFPMMSTFKVLLCGAVLS AmpR C G A T A C A C C G C G C C A T A A T A Amp-R EERFPMMSTFKVLLCGAVLS g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g

5220 85 90 95 100 RIDAGQEQLGRRIHYSQNDL AmpR RIDAGQEQLGRRIHYSQNDL c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c

ScaI 5280 105 110 115 120 VEYSPVTEKHLTDGMTVREL AmpR VEYSPVTEKHLTDGMTVREL g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t

PvuI 5340 125 130 135 140 CSAAITMSDNTAANLLLTTI AmpR CSAAITMSDNTAANLLLTTI t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g

5400 145 150 155 160 GGPKELTAFLHNMGDHVTRL AmpR GGPKELTAFLHNMGDHVTRL g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a

5460 165 170 175 180 DRWEPELNEAIPNDERDTTM AmpR DRWEPELNEAIPNDERDTTM g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c

FspI 5520 185 190 195 200 PVAMATTLRKLLTGELLTLA AmpR PVAMATTLRKLLTGELLTLA c c t g t a g c g a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t g g a c a t c g c t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a

5580 205 210 215 220 SRQQLIDWMEADKVAGPLLR AmpR SRQQLIDWMEADKVAGPLLR t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g

5640 225 230 235 240 SALPAGWFIADKSGAGERGS AmpR SALPAGWFIADKSGAGERGS t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c c g g a g c c g g t g a g c g t g g t t c t a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g g c c t c g g c c a c t c g c a c c a a g a

AfeI 5700 245 250 255 260 RGIIAALGPDGKPSRIVVIY AmpR RGIIAALGPDGKPSRIVVIY c g c g g t a t c a t c g c a g c g c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c g c g c c a t a g t a g c g t c g c g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g

5760 265 270 275 280 TTGSQATMDERNRQIAEIGA AmpR TTGSQATMDERNRQIAEIGA a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g

SalI 5820 285 SLIKHW* AmpR SLIKHW* t c a c t g a t t a a g c a t t g g t a a t g a c c a t g a t t a c g c c a a g c t c t a g c t a g a g g t c g a c g g a g t g a c t a a t t c g t a a c c a t t a c t g g t a c t a a t g c g g t t c g a g a t c g a t c t c c a g c t g c c

BsmI 5880 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev t a t a c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a t a t g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t

5940 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g

6000 SV40 poly(A) signal t g c a a t a a a c a a g t t g g g g t g g g c g a a g a a c t c c a g c a t g a g a t c c c c g c g c t g g a g g a t a c g t t a t t t g t t c a a c c c c a c c c g c t t c t t g a g g t c g t a c t c t a g g g g c g c g a c c t c c t a

6060 c a t c c a g c c g g c g t c c c g g a a a a c g a t t c c g a a g c c c a a c c t t t c a t a g a a g g c g g c g g t g t a g g t c g g c c g c a g g g c c t t t t g c t a a g g c t t c g g g t t g g a a a g t a t c t t c c g c c g c c a

DraIII 6120 200 195 190 *AGPKRTMCWTR PuroR *AGPKRTMCWTR g g a a t c g a a a t c t c g t a g c a c g t g t c a g g c a c c g g g c t t g c g g g t c a t g c a c c a g g t g c g c c t t a g c t t t a g a g c a t c g t g c a c a g t c c g t g g c c c g a a c g c c c a g t a c g t g g t c c a c g c

6180 185 180 175 170 PGEPVEVDATVTFGLREYFP PuroR C G A G C A T C T T C C C Puro-F PGEPVEVDATVTFGLREYFP c g g t c c t t c g g g c a c c t c g a c g t c g g c g g t g a c g g t g a a g c c g a g c c g c t c g t a g a a g g g g c c a g g a a g c c c g t g g a g c t g c a g c c g c c a c t g c c a c t t c g g c t c g g c g a g c a t c t t c c c

6240 165 160 155 150 LNRPASTELFAPVGAREAAE PuroR C T C C A A C G Puro-F LNRPASTELFAPVGAREAAE g a g g t t g c g g g g c g c g g a t g t c t c c a g g a a g g c g g g c a c c c c g g c g c g c t c g g c c g c c t c c t c c a a c g c c c c g c g c c t a c a g a g g t c c t t c c g c c c g t g g g g c c g c g c g a g c c g g c g g a g

6300 145 140 135 130 VGPLVVASGLGKGQHDPSVG PuroR VGPLVVASGLGKGQHDPSVG c a c t c c g g g g a g c a c g a c g g c g c t g c c c a g a c c c t t g c c c t g g t g g t c g g g c g a g a c g c c g t g a g g c c c c t c g t g c t g c c g c g a c g g g t c t g g g a a c g g g a c c a c c a g c c c g c t c t g c g g

6360 125 120 115 110 VTALFWAPEKPRHPALLGEM PuroR VTALFWAPEKPRHPALLGEM g a c g g t g g c c a g g a a c c a c g c g g g c t c c t t g g g c c g g t g c g g c g c c a g g a g g c c t t c c a t c t g c c a c c g g t c c t t g g t g c g c c c g a g g a a c c c g g c c a c g c c g c g g t c c t c c g g a a g g t a

6420 105 100 95 90 QQQAALRSGSLEAMRPGIEA PuroR QQQAALRSGSLEAMRPGIEA c t g t t g c t g c g c g g c c a g c c g g g a a c c g c t c a a c t c g g c c a t g c g c g g g c c g a t c t c g g c g a c a a c g a c g c g c c g g t c g g c c c t t g g c g a g t t g a g c c g g t a c g c g c c c g g c t a g a g c c g

3Ęą 5Ęą 6469 85 80 75 FVAGAEVSEPTTWVAV PuroR FVAGAEVSEPTTWVAV g a a c a c c g c c c c c g c t t c g a c g c t c t c c g g c g t g g t c c a g a c c g c c a c c c t t g t g g c g g g g g c g a a g c t g c g a g a g g c c g c a c c a g g t c t g g c g g t g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.