Addgene pClgn-Tesmin-TurboID-3xFLAG Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pClgn-Tesmin-TurboID-3xFLAG Sequencing Result


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T3 (6212 .. 6232) M13 Reverse (6177 .. 6193) lac operator M13/pUC Reverse (6158 .. 6180) CAP binding site BspQI - SapI (5964) L4440 (5945 .. 5962) pBR322ori-F (5692 .. 5711) AhdI (4959) BsaI (4893) ScaI (4478) TatI (4476) F1ori-F (3883 .. 3904) DraIII (3819) F1ori-R (3673 .. 3692) M13/pUC Forward (3437 .. 3459) M13 Forward (3428 .. 3445) M13 fwd T7 (3399 .. 3418) T7 promoter PacI (3382) BspDI - ClaI (8) KflI (72) BsmBI - Esp3I (199) SacII (290) XbaI (342) BamHI (348) BbvCI (574) TspMI - XmaI (605) SmaI (607) PmlI (668) PaqCI (750) PspXI (1257) BsaBI (1294) NsiI (1452) PfoI * (1711) BmgBI (1943) BclI * (2626) NheI (2757) BmtI (2761) Bsu36I (2851) MscI (2892) BglII (2921) Bglob-pA-R (2924 .. 2943) rbglobpA-R (3043 .. 3062) pClgn-Tesmin-TurboID-3xFLAG 6244 bp

Sequence View

BspDIClaI 60 g a g c t c a t c g a t g a g c t c c a g a g a g g g a c t g a c g t t g g a a g g a c c g c a t c c c c c c a g g c g c t c g a g t a g c t a c t c g a g g t c t c t c c c t g a c t g c a a c c t t c c t g g c g t a g g g g g g t c c g c

KflI 120 c g c g g c c g c t g g g t c c c g a g c g g c g g t t g a g c g g g c c g c t t g c g c a c t g g g g c t g c c a c t g c g c c g g c g a c c c a g g g c t c g c c g c c a a c t c g c c c g g c g a a c g c g t g a c c c c g a c g g t g a

180 g c a t c c t g c a t c c t c c g g c g c g g c c g c t c g c g c a c g c g c a g t t a g c a g t c g c t g c t g g g c c g t a g g a c g t a g g a g g c c g c g c c g g c g a g c g c g t g c g c g t c a a t c g t c a g c g a c g a c c c g

BsmBIEsp3I 240 g g c t g c c g g c g g g a t c g c t c t g g g g a g a c g c c g g g c a c c a a g t a g c g g c c a g t c g g a c a c c c g a c g g c c g c c c t a g c g a g a c c c c t c t g c g g c c c g t g g t t c a t c g c c g g t c a g c c t g t g

SacII 300 c t g a g c t g c c g c t g c t g g a c a c a a g c c c g c a g g a c a g g t g t g t a g a c c g c g g g c c g c c t t g a c t c g a c g g c g a c g a c c t g t g t t c g g g c g t c c t g t c c a c a c a t c t g g c g c c c g g c g g a a

BamHIXbaI 360 Kozak sequence c c t g c g g c t t g t t c t c t g g g g a c c g g g t g a g a a a t c g g a t c t c t a g a g g a t c c g c c g c c a g g a c g c c g a a c a a g a g a c c c c t g g c c c a c t c t t t a g c c t a g a g a t c t c c t a g g c g g c g g t

420 Kozak sequence MEDALLGAMTGPEDELGAEL t g g a g g a c g c g c t g c t c g g c g c c a t g a c t g g c c c c g a a g a c g a g c t g g g c g c c g a g c t g t a c c t c c t g c g c g a c g a g c c g c g g t a c t g a c c g g g g c t t c t g c t c g a c c c g c g g c t c g a c a

480 FGSERVFADGLALSPAGGAA t c g g g t c a g a g c g t g t g t t c g c g g a c g g g c t g g c g c t g a g c c c c g c a g g c g g t g c g g c c g a g c c c a g t c t c g c a c a c a a g c g c c t g c c c g a c c g c g a c t c g g g g c g t c c g c c a c g c c g g c

540 DRDELPVLADAYLGATEPGE a t c g g g a t g a g t t g c c g g t t c t g g c c g a c g c c t a c c t g g g c g c c a c c g a g c c a g g g g a g c t a g c c c t a c t c a a c g g c c a a g a c c g g c t g c g g a t g g a c c c g c g g t g g c t c g g t c c c c t c g

BbvCI 600 PLLRALSPPPGAEVPAALLG c g c t g c t g c g t g c g c t c a g c c c c c c g c c g g g c g c t g a g g t a c c g g c c g c c c t g c t c g g c g g c g a c g a c g c a c g c g a g t c g g g g g g c g g c c c g c g a c t c c a t g g c c g g c g g g a c g a g c c g c

TspMIXmaISmaI 660 DFPGLPELRSPDDAAPPPAY a c t t c c c g g g g c t g c c c g a g c t c c g c a g c c c a g a c g a c g c c g c t c c g c c t c c c g c c t a c a t g a a g g g c c c c g a c g g g c t c g a g g c g t c g g g t c t g c t g c g g c g a g g c g g a g g g c g g a t g t

PmlI 720 SVHVLSSLLPGARGPALLPL g c g t g c a c g t g t t a t c g t c g t t a c t t c c c g g t g c t c g c g g g c c c g c g c t g t t g c c g c t a a c g c a c g t g c a c a a t a g c a g c a a t g a a g g g c c a c g a g c g c c c g g g c g c g a c a a c g g c g a t t

PaqCI 780 SAGVRVIPVEIKEAGGSVPG g c g c a g g a g t g c g c g t g a t c c c a g t t g a a a t c a a a g a a g c a g g t g g t a g t g t g c c a g g c g c g c g t c c t c a c g c g c a c t a g g g t c a a c t t t a g t t t c t t c g t c c a c c a t c a c a c g g t c c g c

840 GSPEDAAFQAPLAQESCCKF g c a g c c c t g a a g a c g c a g c t t t c c a g g c c c c t c t g g c t c a g g a a t c c t g t t g c a a g t t c c c g t c g g g a c t t c t g c g t c g a a a g g t c c g g g g a g a c c g a g t c c t t a g g a c a a c g t t c a a g g

900 PSSQEAEEASSCPRKKDSSP c a t c a t c c c a g g a g g c a g a g g a g g c c t c c a g c t g c c c t c g g a a g a a a g a c t c c a g c c c c a g t a g t a g g g t c c t c c g t c t c c t c c g g a g g t c g a c g g g a g c c t t c t t t c t g a g g t c g g g g t

960 MVICQLKGGAQMLCIDNCGA t g g t g a t t t g t c a g c t g a a a g g a g g c g c c c a g a t g c t c t g c a t a g a c a a c t g t g g c g c g a a c c a c t a a a c a g t c g a c t t t c c t c c g c g g g t c t a c g a g a c g t a t c t g t t g a c a c c g c g c t

1020 RELKALHLLPQYDDQSSFPQ g g g a g c t c a a a g c g c t c c a t c t g c t t c c t c a g t a c g a t g a c c a g a g c a g t t t c c c t c a g t c c c t c g a g t t t c g c g a g g t a g a c g a a g g a g t c a t g c t a c t g g t c t c g t c a a a g g g a g t c a

1080 SELPKPMTTLVGRLLPVPAK c a g a g c t c c c t a a g c c a a t g a c a a c t t t a g t g g g a a g a c t t c t g c c a g t a c c a g c g a a g t g t c t c g a g g g a t t c g g t t a c t g t t g a a a t c a c c c t t c t g a a g a c g g t c a t g g t c g c t t c a

1140 LNLITQVDNGALPSAVNGAA t a a a t c t c a t c a c a c a g g t t g a t a a t g g a g c t c t c c c a t c a g c t g t c a a t g g g g c t g c c t a t t t a g a g t a g t g t g t c c a a c t a t t a c c t c g a g a g g g t a g t c g a c a g t t a c c c c g a c g g a

1200 FPSGPALQGPPKITLSGYCD t t c c c t c t g g a c c t g c t c t g c a a g g g c c a c c c a a a a t a a c t c t g t c t g g g t a c t g t g a c t a a g g g a g a c c t g g a c g a g a c g t t c c c g g t g g g t t t t a t t g a g a c a g a c c c a t g a c a c t g a

PspXI 1260 CFSSGDFCNSCSCNNLRHEL g c t t c t c c a g c g g g g a c t t c t g c a a c a g c t g c a g c t g c a a c a a c c t g c g c c a t g a g c t c g c g a a g a g g t c g c c c c t g a a g a c g t t g t c g a c g t c g a c g t t g t t g g a c g c g g t a c t c g a g c

BsaBI 1320 ERFKAIKACLDRNPEAFQPK a g c g c t t c a a a g c c a t a a a g g c g t g t c t t g a t a g a a a t c c t g a a g c t t t c c a a c c a a a a a t c g c g a a g t t t c g g t a t t t c c g c a c a g a a c t a t c t t t a g g a c t t c g a a a g g t t g g t t t t t

1380 MGKGRLGAAKLRHSKGCNCK t g g g g a a a g g c c g t c t g g g a g c t g c t a a a c t t c g a c a c a g c a a a g g g t g c a a c t g t a a g c a c c c c t t t c c g g c a g a c c c t c g a c g a t t t g a a g c t g t g t c g t t t c c c a c g t t g a c a t t c g

1440 RSGCLKNYCECYEAKIMCSS g c t c a g g c t g c c t g a a g a a c t a c t g t g a g t g c t a t g a g g c c a a a a t c a t g t g t t c t t c c a c g a g t c c g a c g g a c t t c t t g a t g a c a c t c a c g a t a c t c c g g t t t t a g t a c a c a a g a a g g t

NsiI 1500 ICKCIACKNYEESPERKMLM t t t g c a a a t g c a t t g c t t g c a a a a a c t a t g a a g a a a g t c c a g a a c g a a a a a t g c t g a t g a a a a c g t t t a c g t a a c g a a c g t t t t t g a t a c t t c t t t c a g g t c t t g c t t t t t a c g a c t a c t

1560 STPHYMEPGDFESSHYLSPA g c a c a c c c c a c t a c a t g g a g c c t g g g g a c t t t g a g a g c a g c c a t t a t t t g t c c c c a g c c a c g t g t g g g g t g a t g t a c c t c g g a c c c c t g a a a c t c t c g t c g g t a a t a a a c a g g g g t c g g t

1620 KFSGPPKLRKNRQAFSCISW *SRWFQSFIPLGEGTDGP a g t t c t c a g g a c c t c c a a a a c t g a g a a a a a a t a g g c a g g c c t t c t c c t g t a t c t c c t g g g t c a a g a g t c c t g g a g g t t t t g a c t c t t t t t t a t c c g t c c g g a a g a g g a c a t a g a g g a c c c

1680 EVVEATCACLLAQGEEAEQE FYHLGCTGAQQGLTLFCLLL a a g t a g t g g a g g c c a c a t g t g c c t g c c t g c t g g c c c a g g g t g a g g a a g c a g a g c a g g a g c t t c a t c a c c t c c g g t g t a c a c g g a c g g a c g a c c g g g t c c c a c t c c t t c g t c t c g t c c t c g

PfoI* 1740 HCSPSLAEQMILEEFGRCLS VTGWAQSLLHDQLLKSPAQR a c t g t t c c c c a a g c t t g g c t g a g c a g a t g a t c c t g g a g g a g t t t g g a a g g t g c c t g t c g c t g a c a a g g g g t t c g a a c c g a c t c g t c t a c t a g g a c c t c c t c a a a c c t t c c a c g g a c a g c g

1800 TurboID QILHIEFKSKGLKIEVDAAA LNEVDLELGLPQFNLDVRGR a g a t t c t c c a c a t c g a g t t c a a g t c c a a g g g g c t g a a a a t t g a g g t c g a c g c g g c c g c g a t c t a a g a g g t g t a g c t c a a g t t c a g g t t c c c c g a c t t t t a a c t c c a g c t g c g c c g g c g c t

1860 5 10 15 20 KDNTVPLKLIALLANGEFHS TurboID KDNTVPLKLIALLANGEFHS FVISHRQLQDSEQSIALEMT a a g a c a a t a c t g t g c c t c t g a a g c t g a t c g c t c t c c t g g c t a a t g g c g a g t t c c a t a g t g t t c t g t t a t g a c a c g g a g a c t t c g a c t a g c g a g a g g a c c g a t t a c c g c t c a a g g t a t c a c

1920 25 30 35 40 GEQLGETLGMSRAAINKHIQ TurboID GEQLGETLGMSRAAINKHIQ AFLQSFGQAHGPGSDVLVNL g c g a a c a g c t g g g a g a a a c c c t g g g c a t g t c c a g g g c c g c t a t c a a c a a g c a c a t t c a g a c g c t t g t c g a c c c t c t t t g g g a c c c g t a c a g g t c c c g g c g a t a g t t g t t c g t g t a a g t c t

BmgBI 1980 45 50 55 60 TLRDWGVDVFTVPGKGYSLP TurboID TLRDWGVDVFTVPGKGYSLP SQAVPAHVHEGHGSLAVRQG c t c t g c g c g a c t g g g g c g t g g a c g t g t t c a c c g t g c c c g g a a a g g g c t a c t c t c t g c c c g g a g a c g c g c t g a c c c c g c a c c t g c a c a a g t g g c a c g g g c c t t t c c c g a t g a g a g a c g g g c

2040 65 70 75 80 EPIPLLNAKQILGQLDGGSV TurboID EPIPLLNAKQILGQLDGGSV LRDRQQVSFLNQSLQVAPAH a g c c t a t c c c g c t g c t g a a c g c t a a a c a g a t t c t g g g a c a g c t g g a c g g c g g g a g c g t g g t c g g a t a g g g c g a c g a c t t g c g a t t t g t c t a a g a c c c t g t c g a c c t g c c g c c c t c g c a c c

2100 85 90 95 100 AVLPVVDSTNQYLLDRIGEL TurboID AVLPVVDSTNQYLLDRIGEL CDQRHDVGGILVQQISDALQ c a g t c c t g c c t g t g g t c g a c t c c a c c a a t c a g t a c c t g c t g g a t c g a a t c g g c g a g c t g a g t c a g g a c g g a c a c c a g c t g a g g t g g t t a g t c a t g g a c g a c c t a g c t t a g c c g c t c g a c t

2160 105 110 115 120 KSGDACIAEYQQAGRGSRGR TurboID KSGDACIAEYQQAGRGSRGR LTPISANCFILLCPSSASPP a g a g t g g g g a t g c t t g c a t t g c a g a a t a t c a g c a g g c a g g g a g a g g a a g c a g a g g g a g g a t c t c a c c c c t a c g a a c g t a a c g t c t t a t a g t c g t c c g t c c c t c t c c t t c g t c t c c c t c c t

2220 125 130 135 140 KWFSPFGANLYLSMFWRLKR TurboID KWFSPFGANLYLSMFWRLKR FPERRKSSVQVQTHKPAQLP a a t g g t t c t c t c c t t t t g g a g c t a a c c t g t a c c t g a g t a t g t t t t g g c g c c t g a a g c g g g t t a c c a a g a g a g g a a a a c c t c g a t t g g a c a t g g a c t c a t a c a a a a c c g c g g a c t t c g c c c

2280 145 150 155 160 GPAAIGLGPVIGIVMAEALR TurboID GPAAIGLGPVIGIVMAEALR SWCCDAQARDDSNDHCFRQS g a c c a g c a g c a a t c g g c c t g g g c c c g g t c a t c g g a a t t g t c a t g g c a g a a g c g c t g c g a a c t g g t c g t c g t t a g c c g g a c c c g g g c c a g t a g c c t t a a c a g t a c c g t c t t c g c g a c g c t t

2340 165 170 175 180 KLGADKVRVKWPNDLYLQDR TurboID KLGADKVRVKWPNDLYLQDR LQSCVLHSDFPGIVQIQLIS a g c t g g g a g c a g a c a a g g t g c g a g t c a a a t g g c c c a a t g a c c t g t a t c t g c a g g a t a g a a t c g a c c c t c g t c t g t t c c a c g c t c a g t t t a c c g g g t t a c t g g a c a t a g a c g t c c t a t c t t

2400 185 190 195 200 KLAGILVELAGITGDAAQIV TurboID KLAGILVELAGITGDAAQIV LQCADQHLQGSYCAISCLDD a g c t g g c a g g c a t c c t g g t g g a g c t g g c c g g a a t a a c a g g c g a t g c t g c a c a g a t c g t c a t c g a c c g t c c g t a g g a c c a c c t c g a c c g g c c t t a t t g t c c g c t a c g a c g t g t c t a g c a g t

2460 205 210 215 220 IGAGINVAMRRVEESVVNQG TurboID IGAGINVAMRRVEESVVNQG NAGPNVHSHPAHLFAHDILA t t g g c g c c g g g a t t a a c g t g g c t a t g a g g c g c g t g g a g g a a a g c g t g g t c a a t c a g g g c t a a c c g c g g c c c t a a t t g c a c c g a t a c t c c g c g c a c c t c c t t t c g c a c c a g t t a g t c c c g a

2520 225 230 235 240 WITLQEAGINLDRNTLAATL TurboID WITLQEAGINLDRNTLAATL PDCQLFCPNVQVPISQGSRQ g g a t c a c a c t g c a g g a a g c a g g g a t t a a c c t g g a c a g g a a t a c t c t g g c c g c t a c g c t g a c c t a g t g t g a c g t c c t t c g t c c c t a a t t g g a c c t g t c c t t a t g a g a c c g g c g a t g c g a c t

2580 245 250 255 260 IRELRAALELFEQEGLAPYL TurboID IRELRAALELFEQEGLAPYL DSLQPCGQFQELLFAQSWIQ t c c g a g a g c t g c g g g c a g c c c t g g a a c t g t t c g a g c a g g a a g g c c t g g c t c c a t a t c t g c a g g c t c t c g a c g c c c g t c g g g a c c t t g a c a a g c t c g t c c t t c c g g a c c g a g g t a t a g a c g

BclI* 2640 265 270 275 280 PRWEKLDNFINRPVKLIIGD TurboID PRWEKLDNFINRPVKLIIGD WPPLLQIVEDISGHLQDNPV c a c g g t g g g a g a a g c t g g a t a a c t t c a t c a a t a g a c c c g t g a a g c t g a t c a t t g g g g a c a g t g c c a c c c t c t t c g a c c t a t t g a a g t a g t t a t c t g g g c a c t t c g a c t a g t a a c c c c t g t

2700 285 290 295 300 KEIFGISRGIDKQGALLLEQ TurboID KEIFGISRGIDKQGALLLEQ FLNEPNAPPNIFLSGQQQFL a a g a g a t t t t c g g g a t t a g c c g g g g g a t t g a t a a a c a g g g a g c c c t g c t g c t g g a a c a g g t t c t c t a a a a g c c c t a a t c g g c c c c c t a a c t a t t t g t c c c t c g g g a c g a c g a c c t t g t c c

NheI 2760 305 310 315 320 DGVIKPWMGGEISLRSAEK A TurboID DGVIKPWMGGEISLRSAEKA VSNDFGPHASFDTQPRGFLS a c g g a g t t a t c a a a c c c t g g a t g g g c g g a g a a a t c a g t c t g c g g t c t g c c g a a a a g g c t a t g c c t c a a t a g t t t g g g a c c t a c c c g c c t c t t t a g t c a g a c g c c a g a c g g c t t t t c c g a t

BmtI 2820 SGGG 1 5 10 15 DYKDHDGDYKDHDIDY 3xFLAG SGGGDYKDHDGDYKDHDIDY APAPVVFVMVTIIFIMVNIV g c g g g g g c g g g g a c t a c a a a g a c c a t g a c g g t g a t t a t a a a g a t c a t g a c a t t g a t t a c a c g c c c c c g c c c c t g a t g t t t c t g g t a c t g c c a c t a a t a t t t c t a g t a c t g t a a c t a a t g t

Bsu36I 2880 20 KDDDDK * 3xFLAG KDDDDK* LIVIVLLELSRLHLSGILLH a g g a t g a c g a t g a c a a g t a g c t c g a g a c t c c t c a g g t g c a g g c t g c c t a t c a g a a g g t g g t c c t a c t g c t a c t g t t c a t c g a g c t c t g a g g a g t c c a c g t c c g a c g g a t a g t c t t c c a c c

BglIIMscI 2940 A G A A A A A G G G A G A C G G T Bglob-pA-R HSTHGIGQSVFVVSIKKGRG t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a g a t c t t t t t c c c t c t g c c a a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t c t a g a a a a a g g g a g a c g g t

3000 β- globin poly(A) signal T T T Bglob-pA-R FIIPVDHLGKLM a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t t c t g g c t a a t a a a g g a a a t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a a g a c c g a t t a t t t c c t t t a

3060 β- globin poly(A) signal G T G A G C C T T C C T G T A T A C rbglobpA-R t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g a c a t a t g a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c t g t a t a c

3120 C C rbglobpA-R g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t t a g a g t t t g g c a a c a t a t g c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a a t c t c a a a c c g t t g t a t a c

3180 c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a g g t c a t c a g t a t a t g a a a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t c c a g t a g t c a t a t a c t t t g

3240 a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g a c t t g a g g t t a g a t t t t t t t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c t g a a c t c c a a t c t a a a a a a

3300 t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a a a a t t t t c c t t a c a t g t t t a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t t t t a a a a g g a a t g t a c a a a

3360 t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c a t a g c t g t c c c t c t t c t c t a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g t a t c g a c a g g g a g a a g a g a

PacI 3420 T7 promoter G G G A T A T C A C T C A G C A T A A T T7 t a t g a a g a t c c c t c g a c t t a a t t a a g g t a c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g a t a c t t c t a g g g a g c t g a a t t a a t t c c a t g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c

3480 M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g

3540 t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t

3600 f1 ori c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c

3660 f1 ori g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c

3720 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g

3780 f1 ori c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g

DraIII 3840 f1 ori t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t

3900 f1 ori G T G G A C T C T T G T T C C A A A F1ori-F c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t

3960 f1 ori C T G G F1ori-F c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t

4020 f1 ori t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t

4080 f1 ori AmpR promoter a a a t a t t a a c g c t t a c a a t t t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t t a t a a t t g c g a a t g t t a a a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a

4140 AmpR promoter t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t

4200 1 5 10 signal sequence MSIQHFRVAL AmpR promoter AmpR MSIQHFRVAL a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a

4260 15 20 signal sequence IPFFAAFCLPVFA 25 30 HPETLVK AmpR IPFFAAFCLPVFAHPETLVK t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t

4320 35 40 45 50 VKDAEDQLGARVGYIELDLN AmpR VKDAEDQLGARVGYIELDLN a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t

4380 55 60 65 70 SGKILESFRPEERFPMMSTF AmpR SGKILESFRPEERFPMMSTF c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a

4440 75 80 85 90 KVLLCGAVLSRIDAGQEQLG AmpR KVLLCGAVLSRIDAGQEQLG t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c

TatIScaI 4500 95 100 105 110 RRIHYSQNDLVEYSPVTEKH AmpR RRIHYSQNDLVEYSPVTEKH t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t

4560 115 120 125 130 LTDGMTVRELCSAAITMSDN AmpR LTDGMTVRELCSAAITMSDN t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t

4620 135 140 145 150 TAANLLLTTIGGPKELTAFL AmpR TAANLLLTTIGGPKELTAFL c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a

4680 155 160 165 170 HNMGDHVTRLDRWEPELNEA AmpR HNMGDHVTRLDRWEPELNEA g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g

4740 175 180 185 190 IPNDERDTTMPVAMATTLRK AmpR IPNDERDTTMPVAMATTLRK c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t

4800 195 200 205 210 LLTGELLTLASRQQLIDWME AmpR LLTGELLTLASRQQLIDWME a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t

4860 215 220 225 230 ADKVAGPLLRSALPAGWFIA AmpR ADKVAGPLLRSALPAGWFIA g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g

BsaI 4920 235 240 245 250 DKSGAGERGSRGIIAALGPD AmpR DKSGAGERGSRGIIAALGPD t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t

AhdI 4980 255 260 265 270 GKPSRIVVIYTTGSQATMDE AmpR GKPSRIVVIYTTGSQATMDE t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t

5040 275 280 285 RNRQIAEIGASLIKHW* AmpR RNRQIAEIGASLIKHW* a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t

5100 c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a

5160 c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a

5220 ori c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a

5280 ori g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g

5340 ori g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g

5400 ori a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g

5460 ori g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g

5520 ori g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c

5580 ori a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t

5640 ori c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t

5700 ori G G G A A A C G C pBR322ori-F t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g

5760 ori C T G G T A T C T T T pBR322ori-F c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c

5820 ori a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a

5880 c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a

5940 g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t

BspQISapI 6000 A G C G A G T C A G T G A G C G A G L4440 g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g

6060 c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c

6120 -35 CAP binding site lac promoter c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t

6180 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G M13 Reverse c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c

6240 M13 rev T3 promoter A A A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T A A A G G T3 a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t

6244 g c t g c g a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.