Addgene Activin A (S2-2) Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: Activin A (S2-2) Sequencing Result


Map View

NcoI (4312) NdeI (4171) BsrGI (4166) SspI (4104) SV40pA-R (3817 .. 3836) SalI (3769) BstEII (3528) BstAPI (3501) Bpu10I (3398) AsiSI (3381) AseI (2835) ApaLI (2330) BssSI - BssSαI (2189) pBR322ori-F (2157 .. 2176) BmrI (37) AflII (132) PaeR7I - PspXI - XhoI (282) EcoRI (295) NsiI (322) SfiI (337) AgeI (400) MfeI (475) XbaI * (556) BstXI (1249) MreI - NgoMIV (1418) NaeI (1420) EagI - NotI (1784) SacII (1790) EcoRV (1802) DrdI (2124) Activin A (S2-2) 4323 bp

Sequence View

BmrI 60 a c t c t t c a t g g c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t t g c g g a g g a a a t t t g a g a a g t a c c g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a a c g c c t c c t t t a a

120 c t c c g t a a a t g a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g g t a a a a g t t t t g a g a g g c a t t t a c t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c c a t t t t c a a a a c t

AflII 180 a c g c g a g c g a a c t t a a g a g c g c c g g a g t a t a a a t a g a g g c g c t t c g t c t a c g g a g c g a c a t g c g c t c g c t t g a a t t c t c g c g g c c t c a t a t t t a t c t c c g c g a a g c a g a t g c c t c g c t g t

240 a t t c a a t t c a a a c a a g c a a a g t g a a c a c g t c g c t a a g c g a a a g c t a a g c a a a t a a a c a a g t a a g t t a a g t t t g t t c g t t t c a c t t g t g c a g c g a t t c g c t t t c g a t t c g t t t a t t t g t t c

EcoRIPaeR7IPspXIXhoI 300 c g c a g c t g a a c a a g c t a a a c a a t c t g c a g a a g c t t g g t a c c c t c g a g c t c a g c t g a a t t c g c g t c g a c t t g t t c g a t t t g t t a g a c g t c t t c g a a c c a t g g g a g c t c g a g t c g a c t t a a g

SfiINsiI 360 1 5 10 15 MKLCILLAVVAFVGLSL BiP signal sequence MKLCILLAVVAFVGLSL g c c g c c a c c a t g a a g t t a t g c a t a t t a c t g g c c g t c g t g g c c t t t g t t g g c c t c t c g c t c c g g c g g t g g t a c t t c a a t a c g t a t a a t g a c c g g c a g c a c c g g a a a c a a c c g g a g a g c g a g

AgeI 420 G GS 1 5 HHHHHHHH (in frame with 8xHis) LSTGGS DEK BiP signal sequence 8xHis SBP GGSHHHHHHHHLSTGGSDEK g g g g g a t c c c a c c a c c a c c a c c a c c a c c a c c a c c t g t c c a c c g g t g g c a g c g a t g a g a a g c c c c c t a g g g t g g t g g t g g t g g t g g t g g t g g t g g a c a g g t g g c c a c c g t c g c t a c t c t t c

MfeI 480 5 10 15 20 TTGWRGGHVVEGLAGELEQL SBP TTGWRGGHVVEGLAGELEQL a c c a c t g g t t g g a g a g g t g g c c a t g t t g t g g a a g g c t t g g c t g g t g a a t t g g a a c a a t t g t g g t g a c c a a c c t c t c c a c c g g t a c a a c a c c t t c c g a a c c g a c c a c t t a a c c t t g t t a a c

540 25 30 35 RARLEHHPQGQREP GSG 1 LEV SBP HRV 3C site RARLEHHPQGQREPGSGLEV a g a g c c c g t c t g g a g c a t c a c c c a c a a g g c c a a a g a g a a c c a g g c a g c g g c c t g g a a g t g t c t c g g g c a g a c c t c g t a g t g g g t g t t c c g g t t t c t c t t g g t c c g t c g c c g g a c c t t c a c

XbaI* 600 5 LFQGP (in frame with HRV 3C site) SRSPTPGSEGHSAAP HRV 3C site LFQGPSRSPTPGSEGHSAAP c t g t t c c a g g g c c c t t c t a g a t c c c c c a c c c c a g g a t c c g a g g g g c a c a g c g c g g c c c c c g a c a a g g t c c c g g g a a g a t c t a g g g g g t g g g g t c c t a g g c t c c c c g t g t c g c g c c g g g g g

660 (in frame with HRV 3C site) DCPSCALAALPKDVPNSQPE DCPSCALAALPKDVPNSQPE g a c t g t c c g t c c t g t g c g c t g g c c g c c c t c c c a a a g g a t g t a c c c a a c t c t c a g c c a g a g c t g a c a g g c a g g a c a c g c g a c c g g c g g g a g g g t t t c c t a c a t g g g t t g a g a g t c g g t c t c

720 (in frame with HRV 3C site) MVEAVKKHILNMLHLKKRPD MVEAVKKHILNMLHLKKRPD a t g g t g g a g g c c g t c a a g a a g c a c a t t t t a a a c a t g c t g c a c t t g a a g a a g a g a c c c g a t t a c c a c c t c c g g c a g t t c t t c g t g t a a a a t t t g t a c g a c g t g a a c t t c t t c t c t g g g c t a

780 (in frame with HRV 3C site) VTQPVPKAALLNAIRKLHVG VTQPVPKAALLNAIRKLHVG *TP g t c a c c c a g c c g g t a c c c a a g g c g g c g c t t c t g a a c g c g a t c a g a a a g c t t c a t g t g g g c c a g t g g g t c g g c c a t g g g t t c c g c c g c g a a g a c t t g c g c t a g t c t t t c g a a g t a c a c c c g

840 (in frame with HRV 3C site) KVGENGYVEIEDDIGRRAEM KVGENGYVEIEDDIGRRAEM LTPSFPYTSISSSMPLLASI a a a g t c g g g g a g a a c g g g t a t g t g g a g a t a g a g g a t g a c a t t g g a a g g a g g g c a g a a a t g t t t c a g c c c c t c t t g c c c a t a c a c c t c t a t c t c c t a c t g t a a c c t t c c t c c c g t c t t t a c

900 (in frame with HRV 3C site) NELMEQTSEIITFAESGTAR NELMEQTSEIITFAESGTAR FSSISCVESIMVNASDPVAL a a t g a a c t t a t g g a g c a g a c c t c g g a g a t c a t c a c g t t t g c c g a g t c a g g a a c a g c c a g g t t a c t t g a a t a c c t c g t c t g g a g c c t c t a g t a g t g c a a a c g g c t c a g t c c t t g t c g g t c c

960 (in frame with HRV 3C site) KTLHFEISKEGSDLSVVERA KTLHFEISKEGSDLSVVERA FVSCKSIELSPLSRDTTSRA a a g a c g c t g c a c t t c g a g a t t t c c a a g g a a g g c a g t g a c c t g t c a g t g g t g g a g c g t g c a t t c t g c g a c g t g a a g c t c t a a a g g t t c c t t c c g t c a c t g g a c a g t c a c c a c c t c g c a c g t

1020 (in frame with HRV 3C site) EVWLFLKVPKANRTRTKVTI EVWLFLKVPKANRTRTKVTI STQSKRFTGLALLVLVLTVM g a a g t c t g g c t c t t c c t a a a a g t c c c c a a g g c c a a c a g g a c c a g g a c c a a a g t c a c c a t c c t t c a g a c c g a g a a g g a t t t t c a g g g g t t c c g g t t g t c c t g g t c c t g g t t t c a g t g g t a g

1080 (in frame with HRV 3C site) RLFQQQKHPQGSLDTGEEAE RLFQQQKHPQGSLDTGEEAE RRKWCCFCGCPLKSVPSSAS c g c c t c t t c c a g c a g c a g a a g c a c c c g c a g g g c a g c t t g g a c a c a g g g g a a g a g g c c g a g g c g g a g a a g g t c g t c g t c t t c g t g g g c g t c c c g t c g a a c c t g t g t c c c c t t c t c c g g c t c

1140 (in frame with HRV 3C site) EVGLKGERSELLLSEKVVDA EVGLKGERSELLLSEKVVDA STPKFPSLLSSNSESFTTSA *LPLPTFQQERFFYYVS g a a g t g g g c t t a a a g g g g g a g a g g a g t g a a c t g t t g c t c t c t g a a a a a g t a g t a g a c g c t c t t c a c c c g a a t t t c c c c c t c t c c t c a c t t g a c a a c g a g a g a c t t t t t c a t c a t c t g c g a

1200 (in frame with HRV 3C site) RKSTWHVFPVSSSIQRLLDQ RKSTWHVFPVSSSIQRLLDQ RFLVQCTKGTELLMWRNSSW PLAGPMDERDGAADLPQQVL c g g a a g a g c a c c t g g c a t g t c t t c c c t g t c t c c a g c a g c a t c c a g c g g t t g c t g g a c c a g g c c t t c t c g t g g a c c g t a c a g a a g g g a c a g a g g t c g t c g t a g g t c g c c a a c g a c c t g g t c

BstXI 1260 (in frame with HRV 3C site) GKSSLDVRIACEQCQESGAS GKSSLDVRIACEQCQESGAS PLLERSTRIAQSCHWSLPAL ALAGQVNPNGTLLALLTAGA g g c a a g a g c t c c c t g g a c g t t c g g a t t g c c t g t g a g c a g t g c c a g g a g a g t g g c g c c a g c c c g t t c t c g a g g g a c c t g c a a g c c t a a c g g a c a c t c g t c a c g g t c c t c t c a c c g c g g t c g

1320 (in frame with HRV 3C site) LVLLGKKKKKEEEGEGKKKG LVLLGKKKKKEEEGEGKKKG KTRRPLFFFFSSSPSPFFFP QNEQALLLLFFLLPFPFLLA t t g g t t c t c c t g g g c a a g a a g a a g a a g a a a g a a g a g g a g g g g g a a g g g a a a a a g a a g g g c a a c c a a g a g g a c c c g t t c t t c t t c t t c t t t c t t c t c c t c c c c c t t c c c t t t t t c t t c c c g

1380 (in frame with HRV 3C site) GGEGGAGADEEKEQSHRPFL GGEGGAGADEEKEQSHRPFL PPSPPAPASSSFSCDCLGKR STFTPCSCILFLLLRVSREE g g a g g t g a a g g t g g g g c a g g a g c a g a t g a g g a a a a g g a g c a g t c g c a c a g a c c t t t c c t c c c t c c a c t t c c a c c c c g t c c t c g t c t a c t c c t t t t c c t c g t c a g c g t g t c t g g a a a g g a g

NgoMIVMreINaeI 1440 (in frame with HRV 3C site) MLQARQSEDHPHRRRRRGLE MLQARQSEDHPHRRRRRGLE M HQLGPLRFVVRMAPTPPAQL a t g c t g c a g g c c c g g c a g t c t g a a g a c c a c c c t c a t c g c c g g c g t c g g c g g g g c t t g g a g t a c g a c g t c c g g g c c g t c a g a c t t c t g g t g g g a g t a g c g g c c g c a g c c g c c c c g a a c c t c

1500 (in frame with HRV 3C site) CDGKVNICCKKQFFVSFKDI CDGKVNICCKKQFFVSFKDI TIALDVDATLFLEKDTELVD t g t g a t g g c a a g g t c a a c a t c t g c t g t a a g a a a c a g t t c t t t g t c a g t t t c a a g g a c a t c a c a c t a c c g t t c c a g t t g t a g a c g a c a t t c t t t g t c a a g a a a c a g t c a a a g t t c c t g t a g

1560 (in frame with HRV 3C site) GWNDWIIAPSGYHANYCEGE GWNDWIIAPSGYHANYCEGE APIVPDNSGRAIMGVVALTL g g c t g g a a t g a c t g g a t c a t t g c t c c c t c t g g c t a t c a t g c c a a c t a c t g c g a g g g t g a g c c g a c c t t a c t g a c c t a g t a a c g a g g g a g a c c g a t a g t a c g g t t g a t g a c g c t c c c a c t c

1620 (in frame with HRV 3C site) CPSHIAGTSGSSLSFHSTVI CPSHIAGTSGSSLSFHSTVI ARAM t g c c c g a g c c a t a t a g c a g g c a c g t c c g g g t c c t c a c t g t c c t t c c a c t c a a c a g t c a t c a c g g g c t c g g t a t a t c g t c c g t g c a g g c c c a g g a g t g a c a g g a a g g t g a g t t g t c a g t a g

1680 (in frame with HRV 3C site) NHYRMRGHSPFANLKSCCVP NHYRMRGHSPFANLKSCCVP a a c c a c t a c c g c a t g c g g g g c c a t a g c c c c t t t g c c a a c c t c a a a t c g t g c t g t g t g c c c t t g g t g a t g g c g t a c g c c c c g g t a t c g g g g a a a c g g t t g g a g t t t a g c a c g a c a c a c g g g

1740 (in frame with HRV 3C site) TKLRPMSMLYYDDGQNIIKK TKLRPMSMLYYDDGQNIIKK a c c a a g c t g a g a c c c a t g t c c a t g t t g t a c t a t g a t g a t g g t c a a a a c a t c a t c a a a a a g t g g t t c g a c t c t g g g t a c a g g t a c a a c a t g a t a c t a c t a c c a g t t t t g t a g t a g t t t t t c

SacIIEagINotI 1800 (in frame with HRV 3C site) DIQNMIVEECGCS* DIQNMIVEECGCS* g a c a t t c a g a a c a t g a t c g t g g a g g a g t g t g g g t g c t c a t g a g c g g c c g c g g a t c g a t c g c t g t a a g t c t t g t a c t a g c a c c t c c t c a c a c c c a c g a g t a c t c g c c g g c g c c t a g c t a g c

EcoRV 1860 OpIE-2 poly(A) signal a t a t c t g a c t a a a t c t t a g t t t g t a t t g t c a t g t t t t a a t a c a a t a t g t t a t g t t t a a a t t a t a g a c t g a t t t a g a a t c a a a c a t a a c a g t a c a a a a t t a t g t t a t a c a a t a c a a a t t t a

1920 OpIE-2 poly(A) signal a t g t t t t t a a t a a a t t t t a t a a a a t a a t t t c a a c t t t t a t t g t a a c a a c a t t g t c c a t t t t a c a a a a a t t a t t t a a a a t a t t t t a t t a a a g t t g a a a a t a a c a t t g t t g t a a c a g g t a a a

1980 OpIE-2 poly(A) signal a c a c a c t c c t t t c a a g c g c g t g g g a c t c g a t g c t c g g c g c c a c t c a a a g g c g g t a a t a c g t g t g t g a g g a a a g t t c g c g c a c c c t g a g c t a c g a g c c g c g g t g a g t t t c c g c c a t t a t g c

2040 g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t

2100 ori g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t

DrdI 2160 ori T T T C pBR322ori-F c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c

BssSαIBssSI 2220 ori T A T G G T C C G C A A A G G G pBR322ori-F a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a

2280 ori t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c

ApaLI 2340 ori c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g

2400 ori c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a

2460 ori a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t

2520 ori t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g

2580 ori a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g g t c t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c c a g

2640 ori t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a

2700 ori t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g

2760 t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g c a t g c g c t a a g c g g g c t t t a t a a a a c a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c g t a c g c g a t t c g c c c g a a a t a t t t t g

2820 g g g c t g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c t t g t g c t g t g t g g a t a c t c c c c g a c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g a a c a c g a c a c a c c t a t g a g

AseI 2880 c t c c c g a c a c c g a a t t a a t t c g g a t c t c t g c a a g g g a t t t t g g t c a t g a a c a a t a a a a c t g a g g g c t g t g g c t t a a t t a a g c c t a g a g a c g t t c c c t a a a a c c a g t a c t t g t t a t t t t g a

2940 T7 promoter g t c t g c t t a c a t a a a c a g t a a t a c a a g g g g t g t t c a t a g t a t a a t a c g a c t c a c t a t a g g c a g a c g a a t g t a t t t g t c a t t a t g t t c c c c a c a a g t a t c a t a t t a t g c t g a g t g a t a t c c

3000 1 5 10 15 MSHIQRETSCSRPRLNS KanR MSHIQRETSCSRPRLNS a g g g c c a c c a t g a g c c a c a t c c a g c g c g a a a c c a g c t g c a g c c g t c c g c g c c t g a a c a g c t c c c g g t g g t a c t c g g t g t a g g t c g c g c t t t g g t c g a c g t c g g c a g g c g c g g a c t t g t c g

3060 20 25 30 35 NMDADLYGYKWARDNVGQSG KanR NMDADLYGYKWARDNVGQSG a a c a t g g a t g c c g a t c t g t a c g g c t a c a a a t g g g c c c g c g a t a a c g t g g g c c a g a g c g g c t t g t a c c t a c g g c t a g a c a t g c c g a t g t t t a c c c g g g c g c t a t t g c a c c c g g t c t c g c c g

3120 40 45 50 55 ATIYRLYGKPDAPELFLKHG KanR ATIYRLYGKPDAPELFLKHG g c t a c c a t c t a c c g c c t g t a c g g c a a a c c g g a t g c c c c g g a a c t g t t c c t g a a a c a c g g c c g a t g g t a g a t g g c g g a c a t g c c g t t t g g c c t a c g g g g c c t t g a c a a g g a c t t t g t g c c g

3180 60 65 70 75 KGSVANDVTDEMVRLNWLTE KanR KGSVANDVTDEMVRLNWLTE a a a g g c a g c g t g g c c a a c g a t g t g a c c g a t g a a a t g g t g c g c c t g a a c t g g c t g a c c g a g t t t c c g t c g c a c c g g t t g c t a c a c t g g c t a c t t t a c c a c g c g g a c t t g a c c g a c t g g c t c

3240 80 85 90 95 FMPLPTIKHFIRTPDDAWLL KanR FMPLPTIKHFIRTPDDAWLL t t c a t g c c g c t g c c g a c c a t c a a a c a c t t c a t c c g c a c c c c g g a t g a t g c c t g g c t g c t g a a g t a c g g c g a c g g c t g g t a g t t t g t g a a g t a g g c g t g g g g c c t a c t a c g g a c c g a c g a c

3300 100 105 110 115 TTAIPGKTAFQVLEEYPDSG KanR TTAIPGKTAFQVLEEYPDSG a c c a c c g c c a t t c c g g g c a a a a c c g c c t t c c a g g t g c t g g a a g a a t a c c c g g a t a g c g g c t g g t g g c g g t a a g g c c c g t t t t g g c g g a a g g t c c a c g a c c t t c t t a t g g g c c t a t c g c c g

3360 120 125 130 135 ENIVDALAVFLRRLHSIPVC KanR ENIVDALAVFLRRLHSIPVC g a a a a c a t c g t g g a t g c c c t g g c c g t g t t c c t g c g c c g c c t g c a c a g c a t c c c g g t g t g c c t t t t g t a g c a c c t a c g g g a c c g g c a c a a g g a c g c g g c g g a c g t g t c g t a g g g c c a c a c g

Bpu10IAsiSI 3420 140 145 150 155 NCPFNSDRVFRLAQAQSRMN KanR NCPFNSDRVFRLAQAQSRMN a a c t g c c c g t t c a a c a g c g a t c g c g t g t t c c g c c t g g c t c a g g c c c a g a g c c g c a t g a a c t t g a c g g g c a a g t t g t c g c t a g c g c a c a a g g c g g a c c g a g t c c g g g t c t c g g c g t a c t t g

3480 160 165 170 175 NGLVDASDFDDERNGWPVEQ KanR NGLVDASDFDDERNGWPVEQ a a c g g c c t g g t g g a t g c c a g c g a t t t c g a t g a t g a a c g c a a c g g c t g g c c g g t g g a a c a g t t g c c g g a c c a c c t a c g g t c g c t a a a g c t a c t a c t t g c g t t g c c g a c c g g c c a c c t t g t c

BstEIIBstAPI 3540 180 185 190 195 VWKEMHKLLPFSPDSVVTHG KanR VWKEMHKLLPFSPDSVVTHG g t g t g g a a a g a g a t g c a c a a a c t g c t g c c g t t c a g c c c g g a t t c c g t g g t g a c c c a c g g c c a c a c c t t t c t c t a c g t g t t t g a c g a c g g c a a g t c g g g c c t a a g g c a c c a c t g g g t g c c g

3600 200 205 210 215 DFSLDNLIFDEGKLIGCIDV KanR DFSLDNLIFDEGKLIGCIDV g a t t t c a g c c t g g a t a a c c t g a t c t t c g a t g a g g g c a a a c t g a t c g g c t g c a t c g a t g t g c t a a a g t c g g a c c t a t t g g a c t a g a a g c t a c t c c c g t t t g a c t a g c c g a c g t a g c t a c a c

3660 220 225 230 235 GRVGIADRYQDLAILWNCLG KanR GRVGIADRYQDLAILWNCLG g g c c g c g t g g g c a t t g c c g a t c g c t a c c a g g a t c t g g c c a t c c t g t g g a a c t g c c t g g g c c c g g c g c a c c c g t a a c g g c t a g c g a t g g t c c t a g a c c g g t a g g a c a c c t t g a c g g a c c c g

3720 240 245 250 255 EFSPSLQKRLFQKYGIDNPD KanR EFSPSLQKRLFQKYGIDNPD g a g t t c a g c c c g a g c c t g c a g a a a c g c c t g t t c c a g a a g t a c g g c a t c g a t a a c c c g g a t c t c a a g t c g g g c t c g g a c g t c t t t g c g g a c a a g g t c t t c a t g c c g t a g c t a t t g g g c c t a

SalI 3780 260 265 270 MNKLQFHLMLDEFF* KanR SV40 poly(A) signal MNKLQFHLMLDEFF* a t g a a c a a a c t g c a g t t c c a c c t g a t g c t g g a t g a g t t c t t c t a a t a a g t c g a c c t c g a a t a c t t g t t t g a c g t c a a g g t g g a c t a c g a c c t a c t c a a g a a g a t t a t t c a g c t g g a g c t t

3840 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t

3900 SV40 poly(A) signal a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a

3960 SV40 poly(A) signal a t c a t g t c t t c a c g t a a t a a g t g t g c g g c t a g c a g t c a a c t a g t c c a a a c t c a t c a a t g t t a g t a c a g a a g t g c a t t a t t c a c a c g c c g a t c g t c a g t t g a t c a g g t t t g a g t a g t t a c a

4020 a t c t t a t c a t g t c t t c a c g t a a t a a g t g t g c g g c t a g c a g t c a a c t a c t a g t g a a t g c c c t a g a a t a g t a c a g a a g t g c a t t a t t c a c a c g c c g a t c g t c a g t t g a t g a t c a c t t a c g g g

4080 t a c t a g a a g a t g t g t g t t g c a c a a a a t g t c c c t g g a a t a a c c a a t t t g a a g t g c a g a t a g a t g a t c t t c t a c a c a c a a c g t g t t t t a c a g g g a c c t t a t t g g t t a a a c t t c a c g t c t a t c

SspI 4140 c a g t a a a c g t a a g c t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c g c t g g a c a t g t c a t t t g c a t t c g a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a g c g a c c t g t a

NdeIBsrGI 4200 t a c t a a t a a a c c c a c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c a a t g a g t a g t a t g a t t a t t t g g g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g t t a c t c a t c a

4260 t g g g g a a a a a a t g t g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c c a g c t g a a g t a c c c c t t t t t t a c a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g g t c g a c t t c a

NcoI 4320 a t c g a a t a t g a g t a a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a c c c a t g g a a g t a g c t t a t a c t c a t t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t g g g t a c c t t c

4323 t a c a t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.