Addgene pTPH412 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pTPH412 Sequencing Result


Map View

BsrDI (3791) BspHI (3759) pENTR-R (3705 .. 3724) PaeR7I - PspXI - XhoI (3679) Kan-R (3621 .. 3640) BspDI - ClaI (3588) SmaI (3407) TspMI - XmaI (3405) AsiSI - PvuI (3283) BsmBI - Esp3I (3261) HindIII (3159) AseI (3080) PflMI (3021) PacI (2825) FspI (2802) BtgZI (2617) BciVI (2555) PvuII (2540) BsrBI (2524) BsiHKAI - SacI (2472) Eco53kI (2470) EagI (2276) NaeI (2252) MreI - NgoMIV - SgrAI (2250) BaeGI - Bme1580I (2045) MscI * (2034) ScaI (2007) TatI (2005) BssSI - BssSαI (2001) PstI - SbfI (1957) SfiI (3816) SnaBI (184) AflIII - PciI (553) SpeI (1020) BseYI (1204) PspFI (1208) AflII (1311) AlwNI - BstAPI (1585) AhdI (1713) EcoO109I - KflI - PpuMI (1778) BglII (1854) pTPH412 3821 bp

Sequence View

5ʹ 3ʹ 60 315 310 305 300 *IRGYKALINELTTRQKQT Rep101 *IRGYKALINELTTRQKQT g t t c a g a t c c t t c c g t a t t t a g c c a g t a t g t t c t c t a g t g t g g t t c g t t g t t t t t g c g t g c a a g t c t a g g a a g g c a t a a a t c g g t c a t a c a a g a g a t c a c a c c a a g c a a c a a a a a c g c a c

120 295 290 285 280 LWSFSGNLDHKSQMDSLFKA Rep101 LWSFSGNLDHKSQMDSLFKA a g c c a t g a g a a c g a a c c a t t g a g a t c a t g c t t a c t t t g c a t g t c a c t c a a a a a t t t t g c c t c g g t a c t c t t g c t t g g t a a c t c t a g t a c g a a t g a a a c g t a c a g t g a g t t t t t a a a a c g g

180 275 270 265 260 EFSTLQIKATLADHLTKRLG Rep101 EFSTLQIKATLADHLTKRLG t c a a a a c t g g t g a g c t g a a t t t t t g c a g t t a a a g c a t c g t g t a g t g t t t t t c t t a g t c c g a g t t t t g a c c a c t c g a c t t a a a a a c g t c a a t t t c g t a g c a c a t c a c a a a a a g a a t c a g g c

SnaBI 240 255 250 245 240 NRLYSDSTITTPIKDGNMKI Rep101 NRLYSDSTITTPIKDGNMKI t t a c g t a g g t a g g a a t c t g a t g t a a t g g t t g t t g g t a t t t t g t c a c c a t t c a t t t t t a t c a a t g c a t c c a t c c t t a g a c t a c a t t a c c a a c a a c c a t a a a a c a g t g g t a a g t a a a a a t a g

300 235 230 225 220 QNNELETVLDMQRDLEVQFI Rep101 QNNELETVLDMQRDLEVQFI t g g t t g t t c t c a a g t t c g g t t a c g a g a t c c a t t t g t c t a t c t a g t t c a a c t t g g a a a a t c a c c a a c a a g a g t t c a a g c c a a t g c t c t a g g t a a a c a g a t a g a t c a a g t t g a a c c t t t t a g

360 215 210 205 200 LTDTPRGRKDVVLKMNSYTN Rep101 LTDTPRGRKDVVLKMNSYTN a a c g t a t c a g t c g g g c g g c c t c g c t t a t c a a c c a c c a a t t t c a t a t t g c t g t a a g t g t t t t t g c a t a g t c a g c c c g c c g g a g c g a a t a g t t g g t g g t t a a a g t a t a a c g a c a t t c a c a a a

420 195 190 185 180 LDKSIPKLVWQNLRKFEHYN Rep101 LDKSIPKLVWQNLRKFEHYN a a a t c t t t a c t t a t t g g t t t c a a a a c c c a t t g g t t a a g c c t t t t a a a c t c a t g g t a g t t a t t t a g a a a t g a a t a a c c a a a g t t t t g g g t a a c c a a t t c g g a a a a t t t g a g t a c c a t c a a t

480 175 170 165 160 NELMLMFKFEDLSIEINAKH Rep101 NELMLMFKFEDLSIEINAKH t t t t c a a g c a t t a a c a t g a a c t t a a a t t c a t c a a g g c t a a t c t c t a t a t t t g c c t t g t g a a a a a g t t c g t a a t t g t a c t t g a a t t t a a g t a g t t c c g a t t a g a g a t a t a a a c g g a a c a c t

540 155 150 145 140 TKKQTLEKLLWEYIRMSYKN Rep101 TKKQTLEKLLWEYIRMSYKN g t t t t c t t t t g t g t t a g t t c t t t t a a t a a c c a c t c a t a a a t c c t c a t a g a g t a t t t g t t t c a a a a g a a a a c a c a a t c a a g a a a a t t a t t g g t g a g t a t t t a g g a g t a t c t c a t a a a c a a a

AflIIIPciI 600 135 130 125 120 EFSKVHELNYKIFKKLQFLY Rep101 EFSKVHELNYKIFKKLQFLY t c a a a a g a c t t a a c a t g t t c c a g a t t a t a t t t t a t g a a t t t t t t t a a c t g g a a a a g a t a a a g t t t t c t g a a t t g t a c a a g g t c t a a t a t a a a a t a c t t a a a a a a a t t g a c c t t t t c t a t t

660 115 110 105 100 PLIEESFVLELKESSFKAYN Rep101 PLIEESFVLELKESSFKAYN g g c a a t a t c t c t t c a c t a a a a a c t a a t t c t a a t t t t t c g c t t g a g a a c t t g g c a t a g t t t c c g t t a t a g a g a a g t g a t t t t t g a t t a a g a t t a a a a a g c g a a c t c t t g a a c c g t a t c a a a

720 95 90 85 80 TWQFIEFGKVLPNRIEVTRT Rep101 TWQFIEFGKVLPNRIEVTRT g t c c a c t g g a a a a t c t c a a a g c c t t t a a c c a a a g g a t t c c t g a t t t c c a c a g t t c t c g t c c a g g t g a c c t t t t a g a g t t t c g g a a a t t g g t t t c c t a a g g a c t a a a g g t g t c a a g a g c a g

780 75 70 65 60 MLERTAKALVGYANERSINM Rep101 MLERTAKALVGYANERSINM a t c a g c t c t c t g g t t g c t t t a g c t a a t a c a c c a t a a g c a t t t t c c c t a c t g a t g t t c a t c t a g t c g a g a g a c c a a c g a a a t c g a t t a t g t g g t a t t c g t a a a a g g g a t g a c t a c a a g t a g

840 55 50 45 40 MQAYQNYTFSVTREKRTPNE Rep101 MQAYQNYTFSVTREKRTPNE a t c t g a g c g t a t t g g t t a t a a g t g a a c g a t a c c g t c c g t t c t t t c c t t g t a g g g t t t t c a t a g a c t c g c a t a a c c a a t a t t c a c t t g c t a t g g c a g g c a a g a a a g g a a c a t c c c a a a a g t

900 35 30 25 20 ITPNLLAVCCLILKTEHETL Rep101 ITPNLLAVCCLILKTEHETL a t c g t g g g g t t g a g t a g t g c c a c a c a g c a t a a a a t t a g c t t g g t t t c a t g c t c c g t t a a g t a g c a c c c c a a c t c a t c a c g g t g t g t c g t a t t t t a a t c g a a c c a a a g t a c g a g g c a a t t c

960 15 10 5 1 DYRSIALENAKFVVLESM Rep101 DYRSIALENAKFVVLESM t c a t a g c g a c t a a t c g c t a g t t c a t t t g c t t t g a a a a c a a c t a a t t c a g a c a t a c a t c t c a g t a t c g c t g a t t a g c g a t c a a g t a a a c g a a a c t t t t g t t g a t t a a g t c t g t a t g t a g a g

1020 pSC101 ori a a t t g g t c t a g g t g a t t t t a a t c a c t a t a c c a a t t g a g a t g g g c t a g t c a a t g a t a a t t a t t a a c c a g a t c c a c t a a a a t t a g t g a t a t g g t t a a c t c t a c c c g a t c a g t t a c t a t t a a t

SpeI 1080 pSC101 ori c t a g t c c t t t t c c t t t g a g t t g t g g g t a t c t g t a a a t t c t g c t a g a c c t t t g c t g g a a a a g a t c a g g a a a a g g a a a c t c a a c a c c c a t a g a c a t t t a a g a c g a t c t g g a a a c g a c c t t t t

1140 pSC101 ori c t t g t a a a t t c t g c t a g a c c c t c t g t a a a t t c c g c t a g a c c t t t g t g t g t t t t t t t t g t t g a a c a t t t a a g a c g a t c t g g g a g a c a t t t a a g g c g a t c t g g a a a c a c a c a a a a a a a a c a a

1200 pSC101 ori t a t a t t c a a g t g g t t a t a a t t t a t a g a a t a a a g a a a g a a t a a a a a a a g a t a a a a a g a a t a a t a t a a g t t c a c c a a t a t t a a a t a t c t t a t t t c t t t c t t a t t t t t t t c t a t t t t t c t t a t

BseYIPspFI 1260 pSC101 ori g a t c c c a g c c c t g t g t a t a a c t c a c t a c t t t a g t c a g t t c c g c a g t a t t a c a a a a g g a t g c t a g g g t c g g g a c a c a t a t t g a g t g a t g a a a t c a g t c a a g g c g t c a t a a t g t t t t c c t a c

AflII 1320 t c g c a a a c g c t g t t t g c t c c t c t a c a a a a c a g a c c t t a a a a c c c t a a a g g c t t a a g t a g c a g c g t t t g c g a c a a a c g a g g a g a t g t t t t g t c t g g a a t t t t g g g a t t t c c g a a t t c a t c g

1380 a c c c t c g c a a g c t c g g a c a a a t c g c t g a a t a t t c c t t t t g t c t c c g a c c a t c a g g c a c c t t g g g a g c g t t c g a g c c t g t t t a g c g a c t t a t a a g g a a a a c a g a g g c t g g t a g t c c g t g g a

1440 g a g t c g c t g t c t t t t t c g t g a c a t t c a g t t c g c t g c g c t c a c g g c t c t g g c a g t g a a t g g c t c a g c g a c a g a a a a a g c a c t g t a a g t c a a g c g a c g c g a g t g c c g a g a c c g t c a c t t a c c

1500 g g g t a a a t g g c a c t a c a g g c g c c t t t t a t g g a t t c a t g c a a g g a a a c t a c c c a t a a t a c a c c c a t t t a c c g t g a t g t c c g c g g a a a a t a c c t a a g t a c g t t c c t t t g a t g g g t a t t a t g t

1560 a g a a a a g c c c g t c a c g g g c t t c t c a g g g c g t t t t a t g g c g g g t c t g c t a t g t g g t g c t a t t c t t t t c g g g c a g t g c c c g a a g a g t c c c g c a a a a t a c c g c c c a g a c g a t a c a c c a c g a t a

BstAPIAlwNI 1620 c t g a c t t t t t g c t g t t c a g c a g t t c c t g c c c t c t g a t t t t c c a g t c t g a c c a c t t c g g a t g a c t g a a a a a c g a c a a g t c g t c a a g g a c g g g a g a c t a a a a g g t c a g a c t g g t g a a g c c t a

1680 t a t c c c g t g a c a g g t c a t t c a g a c t g g c t a a t g c a c c c a g t a a g g c a g c g g t a t c a t c a a a t a g g g c a c t g t c c a g t a a g t c t g a c c g a t t a c g t g g g t c a t t c c g t c g c c a t a g t a g t t

AhdI 1740 c a g g c t t a c c c g t c t t a c t g t c a a g a g g a c a t c c g g t c a a a t a a a a c g a a a g g c t c a g t c g t c c g a a t g g g c a g a a t g a c a g t t c t c c t g t a g g c c a g t t t a t t t t g c t t t c c g a g t c a g

EcoO109IPpuMIKflI 1800 g a a a g a c t g g g c c t t t c g t t t t g c t g a g g a g a c t t a g g g a c c c t g a t g a a a t t c g c c a c t c t t t c t g a c c c g g a a a g c a a a a c g a c t c c t c t g a a t c c c t g g g a c t a c t t t a a g c g g t g a

BglII 1860 t g t t a g t g t a a t t c g c t a a c t c a t c c t g g c a t g t t g c t g t t g a t t c t t c a a t c a g a t c t c a c a a t c a c a t t a a g c g a t t g a g t a g g a c c g t a c a a c g a c a a c t a a g a a g t t a g t c t a g a g

1920 t a t a a a t c a a a a a g a t a a a a a a t t g g c a c g c a a a t t g t a t t a a c a g t t c a g c a g g a c a a t a t a t t t a g t t t t t c t a t t t t t t a a c c g t g c g t t t a a c a t a a t t g t c a a g t c g t c c t g t t a

SbfIPstI 1980 c c t g a a c g c a g a a a t c a a g a g g a c a a c a t t a c c t g c a g g t g c a g t a a a g g a a a a a a a a a a g g a c t t g c g t c t t t a g t t c t c c t g t t g t a a t g g a c g t c c a c g t c a t t t c c t t t t t t t t t t

MscI*BssSαIBssSITatIScaI 2040 MSEVEFSHEYWMRHALTLAK t g t c t g a g g t g g a g t t t t c c c a c g a g t a c t g g a t g a g a c a t g c c c t g a c c c t g g c c a a g a a c a g a c t c c a c c t c a a a a g g g t g c t c a t g a c c t a c t c t g t a c g g g a c t g g g a c c g g t t c t

Bme1580IBaeGI 2100 RARDEGEVPVGAVLVLNNRV g g g c a c g g g a t g a g g g g g a g g t g c c t g t g g g a g c c g t g c t g g t g c t g a a c a a t a g a g t g a c c c g t g c c c t a c t c c c c c t c c a c g g a c a c c c t c g g c a c g a c c a c g a c t t g t t a t c t c a c t

2160 IGEGWNRAIGLHDPTAHAEI t c g g c g a g g g c t g g a a c a g a g c c a t c g g c c t g c a c g a c c c a a c a g c c c a t g c c g a a a t t a a g c c g c t c c c g a c c t t g t c t c g g t a g c c g g a c g t g c t g g g t t g t c g g g t a c g g c t t t a a t

2220 MALRQGGLVMQNYRLIDATL t g g c c c t g a g a c a g g g c g g c c t g g t c a t g c a g a a c t a c a g a c t g a t t g a c g c c a c c c t g t a c c g g g a c t c t g t c c c g c c g g a c c a g t a c g t c t t g a t g t c t g a c t a a c t g c g g t g g g a c a

EagINgoMIVSgrAIMreINaeI 2280 YVTFEPCVMCAGAMIHSRIG a c g t g a c a t t c g a g c c t t g c g t g a t g t g c g c c g g c g c c a t g a t c c a c t c t a g g a t c g g c c t g c a c t g t a a g c t c g g a a c g c a c t a c a c g c g g c c g c g g t a c t a g g t g a g a t c c t a g c c g g

2340 RVVFGVRNSKRGAAGSLMNV g c g t g g t g t t t g g c g t g a g a a a c t c a a a a a g a g g c g c c g c a g g c t c c c t g a t g a a c g t g c c g c a c c a c a a a c c g c a c t c t t t g a g t t t t t c t c c g c g g c g t c c g a g g g a c t a c t t g c a c g

2400 LNYPGMNHRVEITEGILADE t g a a c t a c c c c g g c a t g a a t c a c c g c g t c g a a a t t a c c g a g g g a a t c c t g g c a g a t g a a t a c t t g a t g g g g c c g t a c t t a g t g g c g c a g c t t t a a t g g c t c c c t t a g g a c c g t c t a c t t a

2460 CAALLCDFYRMPRQVFNAQK g t g c c g c c c t g c t g t g c g a t t t c t a t c g g a t g c c t a g a c a g g t g t t c a a t g c t c a g a a g a c a c g g c g g g a c g a c a c g c t a a a g a t a g c c t a c g g a t c t g t c c a c a a g t t a c g a g t c t t c t

Eco53kIBsiHKAISacI 2520 KAQSSINKESGSVSSEQLAQ a g g c c c a g a g c t c c a t c a a c a a g g a a t c t g g c t c t g t a t c t t c t g a a c a g t t g g c g c a a t t c c g g g t c t c g a g g t a g t t g t t c c t t a g a c c g a g a c a t a g a a g a c t t g t c a a c c g c g t t a

BciVIPvuIIBsrBI 2580 FRSLDSQLNRCLSLDTMVVT t c c g c t c t c t g g a c a g c c a g c t g a a c c g c t g c c t g a g c c t g g a t a c c a t g g t g g t g a c c a a g g c g a g a g a c c t g t c g g t c g a c t t g g c g a c g g a c t c g g a c c t a t g g t a c c a c c a c t g g t

BtgZI 2640 NGKAIEIRDVKVGDWLESEC a c g g c a a a g c g a t t g a a a t t c g c g a t g t g a a a g t g g g c g a t t g g c t g g a a a g c g a a t g c g t g c c g t t t c g c t a a c t t t a a g c g c t a c a c t t t c a c c c g c t a a c c g a c c t t t c g c t t a c g c

2700 GPVQVTEVLPIIKQPVFEIV g c c c g g t g c a g g t g a c c g a a g t g c t g c c g a t t a t t a a a c a g c c g g t g t t t g a a a t t g t g c c g g g c c a c g t c c a c t g g c t t c a c g a c g g c t a a t a a t t t g t c g g c c a c a a a c t t t a a c a c g

2760 LKSGKKIRVSANHKFPTKDG t g a a a a g c g g c a a a a a a a t t c g c g t g a g c g c g a a c c a t a a a t t t c c g a c c a a a g a t g g c c a c t t t t c g c c g t t t t t t t a a g c g c a c t c g c g c t t g g t a t t t a a a g g c t g g t t t c t a c c g g

FspI 2820 LKTINSGLKVGDFLRSRAK* t g a a a a c c a t t a a c a g c g g c c t g a a a g t g g g c g a t t t t c t g c g c a g c c g c g c g a a a t a a c a c t t t t g g t a a t t g t c g c c g g a c t t t c a c c c g c t a a a a g a c g c g t c g g c g c g c t t t a t t g

PacI 2880 t t a a t t a a c g g c a c t c c t c a g c a a a t a t a a t g a c c c t c t t g a t a a c c c a a g a g g g c a t t t a a t t a a t t g c c g t g a g g a g t c g t t t a t a t t a c t g g g a g a a c t a t t g g g t t c t c c c g t a a a

2940 270 265 260 *FFEDLMLHFQLKN KanR 270 265 260 *FFEDLMLHFQLKN KanR *FFEDLMLHFQLKN t t t a a t g c c c a t g g c g t t t a g a a a a a c t c a t c g a g c a t c a a a t g a a a c t g c a a t t t a t t c a a a t t a c g g g t a c c g c a a a t c t t t t t g a g t a g c t c g t a g t t t a c t t t g a c g t t a a a t a a g

3000 255 250 245 240 MDPNDIGYKQFLRKQLSPSF KanR 255 250 245 240 MDPNDIGYKQFLRKQLSPSF KanR MDPNDIGYKQFLRKQLSPSF a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t a a t g a a g g a g a a a a c t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c a t t a c t t c c t c t t t t g

PflMI 3060 235 230 225 220 EGLCNWLIALDQYRDAIGVR KanR 235 230 225 220 EGLCNWLIALDQYRDAIGVR KanR EGLCNWLIALDQYRDAIGVR t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c t g c g a t t c c g a c t c g t a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g a c g c t a a g g c t g a g c a

AseI 3120 215 210 205 200 GVDICGILKGEDFILNDLSF KanR 215 210 205 200 GVDICGILKGEDFILNDLSF KanR GVDICGILKGEDFILNDLSF c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g g t t a t c a a g t g a g a a a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c c a a t a g t t c a c t c t t t

HindIII 3180 195 190 185 180 DGHTVVSDPSFPLLKHMEKW KanR 195 190 185 180 DGHTVVSDPSFPLLKHMEKW KanR DGHTVVSDPSFPLLKHMEKW t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g c t t a t g c a t t t c t t t c c a g a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c g a a t a c g t a a a g a a a g g t c

3240 175 170 165 160 VQEVPWGNREDDFDSADVLG KanR 175 170 165 160 VQEVPWGNREDDFDSADVLG KanR VQEVPWGNREDDFDSADVLG a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t c g c a t c a a c c a a a c c g t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a g c g t a g t t g g t t t g g c

AsiSIPvuIBsmBIEsp3I 3300 155 150 145 140 NNMRSQAQALRFVRDSNFPC KanR 155 150 145 140 NNMRSQAQALRFVRDSNFPC KanR NNMRSQAQALRFVRDSNFPC t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c t g t t a a a a g g a c a a a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g c g a c a a t t t t c c t g t t

3360 135 130 125 120 NCVPISHLRRLFVALADVIN KanR 135 130 125 120 NCVPISHLRRLFVALADVIN KanR NCVPISHLRRLFVALADVIN t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g c a t c a a c a a t a t t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c g c g t a g t t g t t a t a a a

TspMIXmaISmaI 3420 115 110 105 100 EGSDPYEELVQFATKGPIAT KanR 115 110 105 100 EGSDPYEELVQFATKGPIAT KanR EGSDPYEELVQFATKGPIAT t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t c c c g g g g a t c g c a g t g a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a g g g c c c c t a g c g t c a c

3480 95 90 85 80 TLLWADDPTRIFHKITPLPM KanR 95 90 85 80 TLLWADDPTRIFHKITPLPM KanR TLLWADDPTRIFHKITPLPM g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t g g t c g g a a g a g g c a t a c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a c c a g c c t t c t c c g t a t

3540 75 70 65 60 FETLWNLRVMEDTVDNAVSG KanR 75 70 65 60 FETLWNLRVMEDTVDNAVSG KanR FETLWNLRVMEDTVDNAVSG a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c a t t g g c a a c g c t a c c t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g t a a c c g t t g c g a t g g a

BspDIClaI 3600 55 50 45 40 KGHKLFLEPADPKGYLRYIT KanR 55 50 45 40 KGHKLFLEPADPKGYLRYIT KanR KGHKLFLEPADPKGYLRYIT t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a c a a t c g a t a g a t t g t c a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t g t t a g c t a t c t a a c a g

3660 35 30 25 20 AGSQGVNDRAWKYGYLDADM KanR 35 30 25 20 AGSQGVNDRAWKYGYLDADM KanR A T C G C G A G C C C A T T T A T A C C Kan-R AGSQGVNDRAWKYGYLDADM g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a t a a a t c a g c a t c c a t g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t a t t t a g t c g t a g g t a c

PaeR7IPspXIXhoI 3720 15 10 5 1 NSNLRPRSCSTERQIHSM KanR 15 10 5 1 NSNLRPRSCSTERQIHSM KanR A T G G C T C A T A A C A C C C pENTR-R NSNLRPRSCSTERQIHSM t t g g a a t t t a a t c g c g g c c t c g a g c a a g a c g t t t c c c g t t g a a t a t g g c t c a t a a c a c c c a a c c t t a a a t t a g c g c c g g a g c t c g t t c t g c a a a g g g c a a c t t a t a c c g a g t a t t g t g g g

BspHI 3780 C T T G pENTR-R c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a t g a t a t a t t t t t a t c t g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t a c t a t a t a a a a a t a g a

SfiIBsrDI 3ʹ 5ʹ 3821 t g t g c a a t g t a a c a t c a g a g a t t t t g a a g g c c a a a t a g g c c a c a c g t t a c a t t g t a g t c t c t a a a a c t t c c g g t t t a t c c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.