Addgene pAc5.1B-EGFP-DmOskar_C Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAc5.1B-EGFP-DmOskar_C Sequencing Result


Map View

pBRforEco (7836 .. 7854) Amp-R (7426 .. 7445) ScaI (7356) AhdI (6876) pBR322ori-F (6124 .. 6143) DrdI (6091) BfuAI - BspMI (5954) PshAI (5616) SalI (5551) HpaI (5443) SV40pA-R (5387 .. 5406) EBV-rev (5333 .. 5352) BamHI (5308) BGH-rev (5259 .. 5276) PmeI (5243) 6xHis BstBI (5163) SacII (5159) ApaI (5156) PspOMI (5152) XbaI (5146) PaeR7I - PspXI - XhoI (5140) NotI (5134) BspEI (4679) BstXI (4086) XcmI (3815) PflMI * (387) AsiSI (395) TspMI - XmaI (430) SmaI (432) BbvCI (507) NsiI (1418) BspDI - ClaI (1703) SpeI (1953) NdeI (2117) PpuMI (2254) NcoI (2258) Acc65I (2576) KpnI (2580) EGFP-N (2630 .. 2651) EXFP-R (2891 .. 2910) EGFP-C (3238 .. 3259) HindIII (3304) EcoRI (3329) BsaAI - PmlI (3417) BssHII (3790) pAc5.1B-EGFP-DmOskar_C 7862 bp

Sequence View

5ʹ 3ʹ 60 Ac5 promoter c t t c a a g a a t t a a t t c t a t a t t c t a a a a a c a c a a a t g a t a c t t c t a a a a a a a a a t c a t g a g a a g t t c t t a a t t a a g a t a t a a g a t t t t t g t g t t t a c t a t g a a g a t t t t t t t t t a g t a c t

120 Ac5 promoter a t g g c a t c a a c t c t g a a t c a a a t c t t t g c a g a t g c a c c t a c t t c t c a t t t c c a c t g t c a c t a c c g t a g t t g a g a c t t a g t t t a g a a a c g t c t a c g t g g a t g a a g a g t a a a g g t g a c a g t g

180 Ac5 promoter a t c a t t t t t c c a g a t c t c g c t g c c t g t t a t g t g g c c c a c a a a c c a a g a c a c g t t t t a t g g t a g t a a a a a g g t c t a g a g c g a c g g a c a a t a c a c c g g g t g t t t g g t t c t g t g c a a a a t a c c

240 Ac5 promoter c c a t t a a a g c t g g c t g a t c g t c g c c a a a c a c c a a a t a c a t a a t g a a t a t g t a c a c a t t c g g g t a a t t t c g a c c g a c t a g c a g c g g t t t g t g g t t t a t g t a t t a c t t a t a c a t g t g t a a g c

300 Ac5 promoter a g a a a g a a g c g a t c a a a g a a g c g t c t t c g g g c g g a g t a g g a g a a t g c g g a g g a g a a g g a g t c t t t c t t c g c t a g t t t c t t c g c a g a a g c c c g c c t c a t c c t c t t a c g c c t c c t c t t c c t c

360 Ac5 promoter a a c g a g c t g a t c t a g t a t c t c t c c a c a a t c c a a t g c c a a c t g a c c a a c t g g c c a t a t t c g t t g c t c g a c t a g a t c a t a g a g a g g t g t t a g g t t a c g g t t g a c t g g t t g a c c g g t a t a a g c

AsiSIPflMI* 420 Ac5 promoter g a g c a a t t t g a a g c c a a t t t c c a t c g c c t g g c g a t c g c t c c a t t c t t g g c t a t a t g t t t t c t c g t t a a a c t t c g g t t a a a g g t a g c g g a c c g c t a g c g a g g t a a g a a c c g a t a t a c a a a a

TspMIXmaISmaI 480 Ac5 promoter t c a c c g t t a c c c g g g g c c a t t t t c a a a g a c t c g t c g g c a a g a t a a g a t t g t g t c a c t c g c a g t g g c a a t g g g c c c c g g t a a a a g t t t c t g a g c a g c c g t t c t a t t c t a a c a c a g t g a g c g

BbvCI 540 Ac5 promoter t g t c t c t c t t c a t t t g t c g a a g a a t g c t g a g g a a t t t c g c g a t g a c g t c g g c g a g t a t t t a c a g a g a g a a g t a a a c a g c t t c t t a c g a c t c c t t a a a g c g c t a c t g c a g c c g c t c a t a a a

600 Ac5 promoter t g a a g a a t g a g a a t a a t t t g t a t t t a t a c g a a a a t c a g t t a g t g g a a t t t t c t a c a a a a a a c t t c t t a c t c t t a t t a a a c a t a a a t a t g c t t t t a g t c a a t c a c c t t a a a a g a t g t t t t t

660 Ac5 promoter c a t g t t a t c t a t a g a t a a t t t t g t t g c a a a a t a t g t t g a c t a t g a c a a a g a t t g t a t g t a g t a c a a t a g a t a t c t a t t a a a a c a a c g t t t t a t a c a a c t g a t a c t g t t t c t a a c a t a c a t

720 Ac5 promoter t a t a c c t t t a a t g t a t t c t c a t t t t c t t a t g t a t t t a t a a t g g c a a t g a t g a t a c t g a t g a t a t g g a a a t t a c a t a a g a g t a a a a g a a t a c a t a a a t a t t a c c g t t a c t a c t a t g a c t a c

780 Ac5 promoter a t a t t t t a a g a t g a t g c c a g a c c a a a a g g c t t g a a t t t c t g c g t c t t t t g c c g a a c g c a g t a t a a a a t t c t a c t a c g g t c t g g t t t t c c g a a c t t a a a g a c g c a g a a a a c g g c t t g c g t c

840 Ac5 promoter t g c a t g t g c a a t t g t t g t t t t t t g g a a t a t t c a a t t t t c g g a c t g t c c g c t t t g a t t t c a a c g t a c a c g t t a a c a a c a a a a a a c c t t a t a a g t t a a a a g c c t g a c a g g c g a a a c t a a a g t

900 Ac5 promoter M g t t t c t t g g c t t a t t c a a a a a g c a a a g t a a a g c c a a a a a a g c g a g a t g g c a a t a c c a a a t c a a a g a a c c g a a t a a g t t t t t c g t t t c a t t t c g g t t t t t t c g c t c t a c c g t t a t g g t t t a

960 Ac5 promoter RQNGSGRKGVRGSGRKGGAG g c g g c a a a a c g g t a g t g g a a g g a a a g g g g t g c g g g g c a g c g g a a g g a a g g g t g g g g c g g g c g c c g t t t t g c c a t c a c c t t c c t t t c c c c a c g c c c c g t c g c c t t c c t t c c c a c c c c g c c c

1020 Ac5 promoter RGGVCGWARRHRRWSHSFDH g c g t g g c g g g g t c t g t g g c t g g g c g c g a c g t c a c c g a c g t t g g a g c c a c t c c t t t g a c c a c g c a c c g c c c c a g a c a c c g a c c c g c g c t g c a g t g g c t g c a a c c t c g g t g a g g a a a c t g g t

1080 Ac5 promoter VCVCVLFVSRHSPVVFFFLC t g t g t g c g t g t g t g t a t t a t t c g t g t c t c g c c a c t c g c c g g t t g t t t t t t t c t t t t t a t g a c a c a c g c a c a c a c a t a a t a a g c a c a g a g c g g t g a g c g g c c a a c a a a a a a a g a a a a a t a c

1140 Ac5 promoter CALSSAISLTHAQRTACCRF c t g c g c t c t c t c t a g c g c c a t c t c g c t t a c g c a t g c t c a a c g c a c c g c a t g t t g c c g t t t g a c g c g a g a g a g a t c g c g g t a g a g c g a a t g c g t a c g a g t t g c g t g g c g t a c a a c g g c a a a

1200 Ac5 promoter LLCVILARNRQLFKQRLVNE c c t t t t a t g c g t c a t t t t g g c t c g a a a t a g g c a a t t a t t t a a a c a a a g a t t a g t c a a c g a g g a a a a t a c g c a g t a a a a c c g a g c t t t a t c c g t t a a t a a a t t t g t t t c t a a t c a g t t g c t

1260 Ac5 promoter NAKINKSTIWLLIAMCVQPT a a a c g c t a a a a t a a a t a a g t c t a c a a t a t g g t t a c t t a t t g c c a t g t g t g t g c a g c c a a c t t t g c g a t t t t a t t t a t t c a g a t g t t a t a c c a a t g a a t a a c g g t a c a c a c a c g t c g g t t g

1320 Ac5 promoter IATKATTQVAFPLSLFVCKP g a t a g c a a c a a a a g c a a c a a c a c a g g t g g c t t t c c c t c t t t c a c t t t t t g t t t g c a a g c c c t a t c g t t g t t t t c g t t g t t g t g t c c a c c g a a a g g g a g a a a g t g a a a a a c a a a c g t t c g g

1380 Ac5 promoter RASKTARPANAITLTKSRRS g c g t g c g a g c a a g a c g g c a c g a c c g g c a a a c g c a a t t a c g c t g a c a a a g a g c a g a c g a a g c g c a c g c t c g t t c t g c c g t g c t g g c c g t t t g c g t t a a t g c g a c t g t t t c t c g t c t g c t t c

NsiI 1440 Ac5 promoter FGEKHQGA* t t t t g g c g a a a a a c a t c a a g g c g c c t g a t a c g a a t g c a t t t g c a a t a a c a a t t g c g a t a t a a a a c c g c t t t t t g t a g t t c c g c g g a c t a t g c t t a c g t a a a c g t t a t t g t t a a c g c t a t a

1500 Ac5 promoter t t a a t a t t g t t t a t g a a g c t g t t t g a c t t c a a a a c a c a c a a a a a a a a a a a t a a a a c a a a t a a t t a t a a c a a a t a c t t c g a c a a a c t g a a g t t t t g t g t g t t t t t t t t t t t a t t t t g t t t a

1560 Ac5 promoter t a t t t g a a a g a g a a t t a g g a a t c g g a c g c t t a t c g t t a g g g t a a c a a c a a g a a a t g c t t a a t a a a c t t t c t c t t a a t c c t t a g c c t g c g a a t a g c a a t c c c a t t g t t g t t c t t t a c g a a t

1620 Ac5 promoter c t g a g t c a c a g c c t c t g g a a a a c t g c c g c a a g c c a g a g a g a g a g a g a a a a a g a g g g a g a g g a c t c a g t g t c g g a g a c c t t t t g a c g g c g t t c g g t c t c t c t c t c t c t t t t t c t c c c t c t c

1680 Ac5 promoter c a g c t t a g a c c g c a t g t g c t t g t g t g t g a g g c g t c t c t c t c t t c g t c t c t g t t g c g c a a a g t c g a a t c t g g c g t a c a c g a a c a c a c a c t c c g c a g a g a g a g a a g c a g a g a c a a c g c g t t t

BspDIClaI 1740 Ac5 promoter c g c a t a g a c t g c a c t g a a a a a a t c g a t t a c c t a t t t t t t a t g a a t g a a t a t t t g c a c t a t g c g t a t c t g a c g t g a c t t t t t t a g c t a a t g g a t a a a a a a t a c t t a c t t a t a a a c g t g a t a

1800 Ac5 promoter t a c t a t t c a a a a c t a t t a a g a t a g c a a t c a c a t t c a a t a g c c a a a t a c t a t a c c a c c t g a a t g a t a a g t t t t g a t a a t t c t a t c g t t a g t g t a a g t t a t c g g t t t a t g a t a t g g t g g a c t

1860 Ac5 promoter g c g a t g c a a c g a a a t g a t c a a t t t g a g c a a a a a t g c t g c a t a t t t a g g a c g g c a t c a t t a c g c t a c g t t g c t t t a c t a g t t a a a c t c g t t t t t a c g a c g t a t a a a t c c t g c c g t a g t a a t

1920 Ac5 promoter * t a g a a a t g c t t c t t g c t g t g t a c t t t t c t c t c g t c t g g c a g c t g t t t c g c c g t t a t t g t t a t c t t t a c g a a g a a c g a c a c a t g a a a a g a g a g c a g a c c g t c g a c a a a g c g g c a a t a a c a a

SpeI 1980 Ac5 promoter FRSLNPTNEVVLSHGVLLHT a a a a c c g g c t t a a g t t a g g t g t g t t t t c t a c g a c t a g t g a a t g c c c t a c t a g a a g a t g t g t t t t g g c c g a a t t c a a t c c a c a c a a a a g a t g c t g a t c a c t t a c g g g a t g a t c t t c t a c a c

2040 Ac5 promoter NCLIDRSYGIQLASLLLRLS t g t t g c a c a a a a t g t c c c t g g a a t a a c c a a t t t g a a g t g c a g a t a g c a g t a a a c g t a a g c a c a a c g t g t t t t a c a g g g a c c t t a t t g g t t a a a c t t c a c g t c t a t c g t c a t t t g c a t t c g

2100 Ac5 promoter IHINNLQLTKIDSSMVLLGV t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c g c t g g a c a t t a c t a a t a a a c c c a a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a g c g a c c t g t a a t g a t t a t t t g g g t

NdeI 2160 Ac5 promoter IFVHVYTHKPMCHTTPSFIH c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c a a t g a g t a g t t g g g g a a a a a a t g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g t t a c t c a t c a a c c c c t t t t t t a c a

2220 Ac5 promoter LLVTVMVAYLYGASTDFILL g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c c a g c t g a a g t a t c g a a t a t g a g t a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g g t c g a c t t c a t a g c t t a t a c t c a t

PpuMINcoI 2280 Ac5 promoter GWISDCAAVSLVWPLVSKMA a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a c c c a t g g a a g t a c a c t c t t c a t g g t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t g g g t a c c t t c a t g t g a g a a g t a c c

2340 Ac5 promoter IYLVCVLVFVWNRLFNETFS c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t t g c g g a g g a a a t t c t c c g t a a a t g g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a a c g c c t c c t t t a a g a g g c a t t t a c

2400 Ac5 promoter FGIPSCNMGMYPLLKSRSKL a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g g t a a a a g t t t t g a a c g c g a c t t g a t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c c a t t t t c a a a a c t t g c g c t g a a c t

2460 Ac5 promoter ASLM g a g c g g a g a g c a t t g c g g c t g a t a a g g t t t t a g c g c t a a g c g g g c t t t a t a a a a c g g g c t c t c g c c t c t c g t a a c g c c g a c t a t t c c a a a a t c g c g a t t c g c c c g a a a t a t t t t g c c c g a

2520 Ac5 promoter g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c t t g t g c t g t g t g g a t a c t c c t c c c c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g a a c a c g a c a c a c c t a t g a g g a g g g

Acc65IKpnI 2580 Ac5 promoter g a c a c a a a g c c g c t c c a t c a g c c a g c a g t c g t c t a a t c c a g a g a c c c c g g a t c g g g g t a c c t g t g t t t c g g c g a g g t a g t c g g t c g t c a g c a g a t t a g g t c t c t g g g g c c t a g c c c c a t g

2640 1 M 1a V 5 10 15 SKGEELFTGVVPILVEL EGFP 1 M 1a V 5 10 15 SKGEELFTGVVPILVEL EGFP G A C C A G C T C G A EGFP-N MVSKGEELFTGVVPILVEL c a t c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g t a g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a

2700 20 25 30 35 DGDVNGHKFSVSGEGEGDAT EGFP 20 25 30 35 DGDVNGHKFSVSGEGEGDAT EGFP C C T G C C G C T G C EGFP-N DGDVNGHKFSVSGEGEGDAT g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g

2760 40 45 50 55 YGKLTLKFICTTGKLPVPWP EGFP 40 45 50 55 YGKLTLKFICTTGKLPVPWP EGFP YGKLTLKFICTTGKLPVPWP c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g

2820 60 65 70 75 TLVTTLTYGVQCFSRYPDHM EGFP 60 65 70 75 TLVTTLTYGVQCFSRYPDHM EGFP TLVTTLTYGVQCFSRYPDHM c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a

2880 80 85 90 95 KQHDFFKSAMPEGYVQERTI EGFP 80 85 90 95 KQHDFFKSAMPEGYVQERTI EGFP KQHDFFKSAMPEGYVQERTI g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a

2940 100 105 110 115 FFKDDGNYKTRAEVKFEGDT EGFP 100 105 110 115 FFKDDGNYKTRAEVKFEGDT EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R FFKDDGNYKTRAEVKFEGDT c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g

3000 120 125 130 135 LVNRIELKGIDFKEDGNILG EGFP 120 125 130 135 LVNRIELKGIDFKEDGNILG EGFP LVNRIELKGIDFKEDGNILG c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c

3060 140 145 150 155 HKLEYNYNSHNVYIMADKQK EGFP 140 145 150 155 HKLEYNYNSHNVYIMADKQK EGFP HKLEYNYNSHNVYIMADKQK g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t

3120 160 165 170 175 NGIKVNFKIRHNIEDGSVQL EGFP 160 165 170 175 NGIKVNFKIRHNIEDGSVQL EGFP NGIKVNFKIRHNIEDGSVQL g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a

3180 180 185 190 195 ADHYQQNTPIGDGPVLLPDN EGFP 180 185 190 195 ADHYQQNTPIGDGPVLLPDN EGFP ADHYQQNTPIGDGPVLLPDN c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t

3240 200 205 210 215 HYLSTQSALSKDPNEKRDHM EGFP 200 205 210 215 HYLSTQSALSKDPNEKRDHM EGFP C A T EGFP-C HYLSTQSALSKDPNEKRDHM c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a

3300 220 225 230 235 VLLEFVTAAGITLGMDELYK EGFP 220 225 230 235 VLLEFVTAAGITLGMDELYK EGFP G G T C C T G C T G G A G T T C G T G EGFP-C VLLEFVTAAGITLGMDELYK g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t

EcoRIHindIII 3360 (in frame with EGFP) PSLMAAVTSEFPSKPISYTS EGFP (in frame with EGFP) PSLMAAVTSEFPSKPISYTS EGFP PSLMAAVTSEFPSKPISYTS g c c a a g c t t g a t g g c c g c a g t c a c a a g t g a a t t c c c c a g c a a a c c g a t c a g t t a t a c c a g c g g t t c g a a c t a c c g g c g t c a g t g t t c a c t t a a g g g g t c g t t t g g c t a g t c a a t a t g g t c

BsaAIPmlI 3420 (in frame with EGFP) TNTSAKTYYLKSVKKRVTTC (in frame with EGFP) TNTSAKTYYLKSVKKRVTTC TNTSAKTYYLKSVKKRVTTC c a c c a a t a c t t c c g c c a a a a c c t a t t a t c t t a a g t c c g t g a a a a a g c g g g t g a c c a c g t g g t g g t t a t g a a g g c g g t t t t g g a t a a t a g a a t t c a g g c a c t t t t t c g c c c a c t g g t g c a c

3480 (in frame with EGFP) FQQLRDKLQSSGSFRKSSSS (in frame with EGFP) FQQLRDKLQSSGSFRKSSSS FQQLRDKLQSSGSFRKSSSS *GTGKALTGGA t t t c c a g c a g t t g c g c g a t a a a c t c c a g t c a t c c g g t t c c t t t c g c a a g a g t t c c t c c a g a a a g g t c g t c a a c g c g c t a t t t g a g g t c a g t a g g c c a a g g a a a g c g t t c t c a a g g a g g t c

3540 (in frame with EGFP) CLNQIFVRSDFSACGERFRK (in frame with EGFP) CLNQIFVRSDFSACGERFRK CLNQIFVRSDFSACGERFRK AEVLDEYPGIEGCAAFAKPL c t g c c t c a a c c a g a t c t t c g t a a g g t c c g a t t t c t c c g c a t g c g g c g a a c g c t t t c g g a a g a c g g a g t t g g t c t a g a a g c a t t c c a g g c t a a a g a g g c g t a c g c c g c t t g c g a a a g c c t t

3600 (in frame with EGFP) IFKSARKTELPELWKVPLVA (in frame with EGFP) IFKSARKTELPELWKVPLVA IFKSARKTELPELWKVPLVA DEFRRSFGFQRLQPLHWQHG g a t c t t c a a a t c g g c g c g a a a a a c c g a a t t g c c g g a g t t g t g g a a g g t g c c a t t g g t g g c c t a g a a g t t t a g c c g c g c t t t t t g g c t t a a c g g c c t c a a c a c c t t c c a c g g t a a c c a c c g

3660 (in frame with EGFP) HELTSRQSSQQLQVVARLFS (in frame with EGFP) HELTSRQSSQQLQVVARLFS HELTSRQSSQQLQVVARLFS MFEGAPLAALLQLDYGSEER c c a t g a a c t c a c c a g c c g g c a g a g c a g c c a g c a g t t g c a a g t c g t a g c c a g a c t c t t c t c g g t a c t t g a g t g g t c g g c c g t c t c g t c g g t c g t c a a c g t t c a g c a t c g g t c t g a g a a g a g

3720 (in frame with EGFP) STQISTKEITYNSNSNTSEN (in frame with EGFP) STQISTKEITYNSNSNTSEN STQISTKEITYNSNSNTSEN GSLNGGLFDGVVAVAVGALV g t c c a c t c a g a t t t c c a c c a a g g a a a t c a c c t a c a a c a g c a a c a g c a a c a c c a g c g a g a a c a g g t g a g t c t a a a g g t g g t t c c t t t a g t g g a t g t t g t c g t t g t c g t t g t g g t c g c t c t t

3780 (in frame with EGFP) NMTIIESNYISVREEYPDID (in frame with EGFP) NMTIIESNYISVREEYPDID NMTIIESNYISVREEYPDID VHGDDLAVIYGHALFIGIYI c a a c a t g a c c a t c a t c g a g a g c a a c t a t a t a t c c g t g c g c g a g g a a t a t c c c g a t a t a g a g t t g t a c t g g t a g t a g c t c t c g t t g a t a t a t a g g c a c g c g c t c c t t a t a g g g c t a t a t c t

XcmIBssHII 3840 (in frame with EGFP) SEVRAILLSHAQNGITISSI (in frame with EGFP) SEVRAILLSHAQNGITISSI SEVRAILLSHAQNGITISSI TLHAGYQQAVGLISDRYRAD t a g t g a g g t g c g c g c c a t a t t g c t g a g c c a c g c c c a g a a t g g a a t c a c g a t a t c g a g c a t a t c a c t c c a c g c g c g g t a t a a c g a c t c g g t g c g g g t c t t a c c t t a g t g c t a t a g c t c g t a

3900 (in frame with EGFP) KSEYRKLTGNPFPLHDNVTD (in frame with EGFP) KSEYRKLTGNPFPLHDNVTD KSEYRKLTGNPFPLHDNVTD LTFISFQRAIWKWQVVVHRI c a a g a g t g a a t a t c g a a a a c t g a c g g g c a a t c c a t t t c c a c t g c a c g a c a a c g t g a c g g a g t t c t c a c t t a t a g c t t t t g a c t g c c c g t t a g g t a a a g g t g a c g t g c t g t t g c a c t g c c t

3960 (in frame with EGFP) FLLTIPNVTAECSESGKRIF (in frame with EGFP) FLLTIPNVTAECSESGKRIF FLLTIPNVTAECSESGKRIF EQQGNGIHGSLAALGTLPDE t t t c c t g c t g a c c a t t c c c a a t g t g a c c g c t g a g t g c a g c g a g t c c g g t a a g c g g a t c t t a a a g g a c g a c t g g t a a g g g t t a c a c t g g c g a c t c a c g t c g c t c a g g c c a t t c g c c t a g a a

4020 (in frame with EGFP) NLKASLKNGHLLDMVLNQKE (in frame with EGFP) NLKASLKNGHLLDMVLNQKE NLKASLKNGHLLDMVLNQKE VQFRAQLVTVEQIHHEVLFL c a a c c t g a a a g c g a g c c t g a a g a a c g g t c a c c t c c t g g a t a t g g t g c t c a a c c a g a a a g a g t t g g a c t t t c g c t c g g a c t t c t t g c c a g t g g a g g a c c t a t a c c a c g a g t t g g t c t t t c t

4080 (in frame with EGFP) RTSDYSSGAPSLENIPRAPP (in frame with EGFP) RTSDYSSGAPSLENIPRAPP RTSDYSSGAPSLENIPRAPP AGAVVAASSRGQLVYWSCRW g c g c a c c a g c g a c t a c a g c a g c g g a g c t c c g t c c c t g g a g a a c a t a c c a c g a g c a c c t c c c g c g t g g t c g c t g a t g t c g t c g c c t c g a g g c a g g g a c c t c t t g t a t g g t g c t c g t g g a g g

BstXI 4140 (in frame with EGFP) RYWKNPFKRRALSQLNTSPR (in frame with EGFP) RYWKNPFKRRALSQLNTSPR RYWKNPFKRRALSQLNTSPR AVPLIGEFPPSQGLQVGARP a c g c t a c t g g a a g a a t c c c t t c a a a c g g a g g g c t c t g t c c c a g c t g a a c a c c a g c c c g a g t g c g a t g a c c t t c t t a g g g a a g t t t g c c t c c c g a g a c a g g g t c g a c t t g t g g t c g g g c t c

4200 (in frame with EGFP) TVPKITDEKTKDIATRPVSL (in frame with EGFP) TVPKITDEKTKDIATRPVSL TVPKITDEKTKDIATRPVSL GHGLYRIFLGLIDGGPRNRQ g a c c g t g c c c a a g a t a a c g g a t g a a a a g a c c a a g g a t a t c g c c a c c a g g c c g g t t t c g c t c t g g c a c g g g t t c t a t t g c c t a c t t t t c t g g t t c c t a t a g c g g t g g t c c g g c c a a a g c g a

4260 (in frame with EGFP) HQMANEAAESNWCYQDNWKH (in frame with EGFP) HQMANEAAESNWCYQDNWKH HQMANEAAESNWCYQDNWKH MLHGILCRLRVPAVLIIPLM g c a t c a a a t g g c c a a t g a g g c a g c g g a g t c g a a c t g g t g c t a c c a g g a t a a t t g g a a g c a c g t a g t t t a c c g g t t a c t c c g t c g c c t c a g c t t g a c c a c g a t g g t c c t a t t a a c c t t c g t

4320 (in frame with EGFP) LNNFYQQASVNAPKMPVPIN (in frame with EGFP) LNNFYQQASVNAPKMPVPIN LNNFYQQASVNAPKMPVPIN EVIEVLLGAYIRWFHWYGDV t c t c a a c a a t t t c t a c c a g c a a g c c a g c g t a a a t g c g c c a a a a a t g c c a g t a c c c a t c a a a g a g t t g t t a a a g a t g g t c g t t c g g t c g c a t t t a c g c g g t t t t t a c g g t c a t g g g t a g t t

4380 (in frame with EGFP) IYSPDAPEEPINLAPPGHQP (in frame with EGFP) IYSPDAPEEPINLAPPGHQP IYSPDAPEEPINLAPPGHQP DVAGIGWLFWDIQSWRPMLW c a t c t a c a g c c c c g a t g c c c c a g a g g a a c c a a t c a a t t t g g c t c c a c c t g g g c a t c a g c c g t a g a t g t c g g g g c t a c g g g g t c t c c t t g g t t a g t t a a a c c g a g g t g g a c c c g t a g t c g g

4440 (in frame with EGFP) SCRTQSQKTEPTENRHLGIF (in frame with EGFP) SCRTQSQKTEPTENRHLGIF SCRTQSQKTEPTENRHLGIF AASGLALFGFRSFVAMQADK a a g c t g c a g a a c c c a a a g c c a g a a a a c c g a a c c g a c t g a a a a c c g c c a t t t g g g c a t c t t t t c g a c g t c t t g g g t t t c g g t c t t t t g g c t t g g c t g a c t t t t g g c g g t a a a c c c g t a g a a

4500 (in frame with EGFP) VHPFNGMNIMKRRHEMTPTP (in frame with EGFP) VHPFNGMNIMKRRHEMTPTP VHPFNGMNIMKRRHEMTPTP HM t g t g c a t c c a t t t a a c g g c a t g a a c a t a a t g a a g a g a c g c c a c g a a a t g a c g c c c a c g c c a c a c g t a g g t a a a t t g c c g t a c t t g t a t t a c t t c t c t g c g g t g c t t t a c t g c g g g t g c g g

4560 (in frame with EGFP) TILTSGTYNDSLLTINSDYD (in frame with EGFP) TILTSGTYNDSLLTINSDYD TILTSGTYNDSLLTINSDYD a a c g a t t t t a a c c a g t g g a a c c t a c a a c g a t t c t c t g c t g a c g a t t a a c t c g g a t t a c g a t t g c t a a a a t t g g t c a c c t t g g a t g t t g c t a a g a g a c g a c t g c t a a t t g a g c c t a a t g c t

4620 (in frame with EGFP) AYLLDFPLMGDDFMLYLARM (in frame with EGFP) AYLLDFPLMGDDFMLYLARM AYLLDFPLMGDDFMLYLARM t g c c t a t c t g c t g g a c t t t c c g c t t a t g g g c g a t g a t t t t a t g c t a t a t c t c g c c c g a a t a c g g a t a g a c g a c c t g a a a g g c g a a t a c c c g c t a c t a a a a t a c g a t a t a g a g c g g g c t t a

BspEI 4680 (in frame with EGFP) ELKCRFRRHERVLQSGLCVS (in frame with EGFP) ELKCRFRRHERVLQSGLCVS ELKCRFRRHERVLQSGLCVS g g a g c t a a a a t g c c g a t t t a g g c g t c a c g a a c g c g t c c t g c a g t c a g g a c t t t g t g t a t c c c t c g a t t t t a c g g c t a a a t c c g c a g t g c t t g c g c a g g a c g t c a g t c c t g a a a c a c a t a g

4740 (in frame with EGFP) GLTINGARNRLKRVQLPEGT (in frame with EGFP) GLTINGARNRLKRVQLPEGT GLTINGARNRLKRVQLPEGT c g g a c t g a c g a t c a a t g g c g c c c g a a a t c g t t t a a a a a g a g t c c a a t t a c c c g a g g g t a c g c c t g a c t g c t a g t t a c c g c g g g c t t t a g c a a a t t t t t c t c a g g t t a a t g g g c t c c c a t g

4800 (in frame with EGFP) QIIVNIGSVDIMRGKPLVQI (in frame with EGFP) QIIVNIGSVDIMRGKPLVQI QIIVNIGSVDIMRGKPLVQI t c a g a t c a t c g t c a a t a t c g g a t c g g t g g a c a t t a t g c g c g g c a a g c c t t t g g t t c a g a t a g t c t a g t a g c a g t t a t a g c c t a g c c a c c t g t a a t a c g c g c c g t t c g g a a a c c a a g t c t a

4860 (in frame with EGFP) EHDFRLLIKEMHNMRLVPIL (in frame with EGFP) EHDFRLLIKEMHNMRLVPIL EHDFRLLIKEMHNMRLVPIL c g a g c a c g a t t t t c g g c t a c t g a t c a a g g a g a t g c a c a a t a t g c g a t t g g t g c c g a t t c t g c t c g t g c t a a a a g c c g a t g a c t a g t t c c t c t a c g t g t t a t a c g c t a a c c a c g g c t a a g a

4920 (in frame with EGFP) TNLAPLGNYCHDKVLCDKIY (in frame with EGFP) TNLAPLGNYCHDKVLCDKIY TNLAPLGNYCHDKVLCDKIY a a c a a a t c t t g c a c c g c t g g g c a a c t a t t g t c a c g a t a a g g t a t t a t g t g a c a a a a t c t a t t g t t t a g a a c g t g g c g a c c c g t t g a t a a c a g t g c t a t t c c a t a a t a c a c t g t t t t a g a t

4980 (in frame with EGFP) RFNKFIRSECCHLKVIDIHS (in frame with EGFP) RFNKFIRSECCHLKVIDIHS RFNKFIRSECCHLKVIDIHS c c g a t t c a a c a a g t t t a t c c g a a g c g a a t g c t g t c a c c t a a a g g t c a t c g a c a t a c a c t c g g c t a a g t t g t t c a a a t a g g c t t c g c t t a c g a c a g t g g a t t t c c a g t a g c t g t a t g t g a g

5040 (in frame with EGFP) CLINERGVVRFDCFQASPRQ (in frame with EGFP) CLINERGVVRFDCFQASPRQ CLINERGVVRFDCFQASPRQ c t g t c t a a t c a a c g a a a g g g g c g t g g t g c g a t t t g a t t g c t t t c a g g c c t c a c c a c g c c a g a c a g a t t a g t t g c t t t c c c c g c a c c a c g c t a a a c t a a c g a a a g t c c g g a g t g g t g c g g t

5100 (in frame with EGFP) VTGSKEPYLFWNKIGRQRVL (in frame with EGFP) VTGSKEPYLFWNKIGRQRVL VTGSKEPYLFWNKIGRQRVL a g t c a c c g g t t c c a a g g a a c c c t a t c t g t t c t g g a a c a a a a t c g g t c g g c a g c g c g t a c t t c a g t g g c c a a g g t t c c t t g g g a t a g a c a a g a c c t t g t t t t a g c c a g c c g t c g c g c a t g a

NotIPaeR7IPspXIXhoIXbaIPspOMIApaISacII 5160 (in frame with EGFP) QVIETSLEY* (in frame with EGFP) QVIETSLEY* QVIETSLEY* g c a a g t t a t t g a a a c g a g t c t g g a g t a t t a a g g c g g c c g c t c g a g t c t a g a g g g c c c g c g c g t t c a a t a a c t t t g c t c a g a c c t c a t a a t t c c g c c g g c g a g c t c a g a t c t c c c g g g c g c

BstBI 5220 1 5 10 GKPIPNPLLGLDST RTG 1 H V5 tag 6xHis g t t c g a a g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c g c g t a c c g g t c a c a a g c t t c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g c g c a t g g c c a g t

PmeI 5280 5 HHHHH * 6xHis G G A G C T G A C A C G G A A G A T BGH-rev t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a a g g c a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t t c c g

BamHI 5340 SV40 poly(A) signal C T A C T C A A EBV-rev c t g a g c t c g c t g a t c a g c c t c g a t c g a g g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t g a c t c g a g c g a c t a g t c g g a g c t a g c t c c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a

5400 SV40 poly(A) signal G A A A T T T G T G A T G C SV40pA-R A C C T G T T T G G T G EBV-rev t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g

HpaI 5460 SV40 poly(A) signal T A T T G C SV40pA-R t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a

5520 *TEPEPPPTPPKKFCTFG t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a

SalI 5580 MISRPMPLRAFN RCIHYPQNHDTSRHGQSGEV c t a c a a a t g t g g t a t g g c t g a t t a t g a t c a g t c g a c c g a t g c c c t t g a g a g c c t t c a a c c g a t g t t t a c a c c a t a c c g a c t a a t a c t a g t c a g c t g g c t a c g g g a a c t c t c g g a a g t t g g

PshAI 5640 PVSSFRWARGMTIVAALMTV WDAGEPPRPAHSDDGCKHSD c a g t c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t a t c g t c g c c g c a c t t a t g a c t g t c t g t c a g t c g a g g a a g g c c a c c c g c g c c c c g t a c t g a t a g c a g c g g c g t g a a t a c t g a c a g a

5700 FFIMQLVGQVPAALWVIFGE EKDHLEYSLHRCREPDNEAL t c t t t a t c a t g c a a c t c g t a g g a c a g g t g c c g g c a g c g c t c t g g g t c a t t t t c g g c g a g g a g a a a t a g t a c g t t g a g c a t c c t g t c c a c g g c c g t c g c g a g a c c c a g t a a a a g c c g c t c c

5760 DRFRWSATMIGLSLAVFGIL VAKAPARRHDAQRKRYESDQ a c c g c t t t c g c t g g a g c g c g a c g a t g a t c g g c c t g t c g c t t g c g g t a t t c g g a a t c t t g c t g g c g a a a g c g a c c t c g c g c t g c t a c t a g c c g g a c a g c g a a c g c c a t a a g c c t t a g a a c g

5820 HALAQAFVTGPATKRFGEKQ VGESLGEDSTGGGFTEALLL a c g c c c t c g c t c a a g c c t t c g t c a c t g g t c c c g c c a c c a a a c g t t t c g g c g a g a a g c a g g t g c g g g a g c g a g t t c g g a a g c a g t g a c c a g g g c g g t g g t t t g c a a a g c c g c t c t t c g t c c

5880 AIIAGMAADALGYVLLAFAT GNDGAHRGVRQAVDQQRERR c c a t t a t c g c c g g c a t g g c g g c c g a c g c g c t g g g c t a c g t c t t g c t g g c g t t c g c g a c g c g g t a a t a g c g g c c g t a c c g c c g g c t g c g c g a c c c g a t g c a g a a c g a c c g c a a g c g c t g c g

5940 RGWMAFPIMILLASGGIGMP SAPHGEGNHNKESGAADPHG g a g g c t g g a t g g c c t t c c c c a t t a t g a t t c t t c t c g c t t c c g g c g g c a t c g g g a t g c c c g c t c c g a c c t a c c g g a a g g g g t a a t a c t a a g a a g a g c g a a g g c c g c c g t a g c c c t a c g g g c

BspMIBfuAI 6000 ALQAMLSRQVDDDHQGQLQG RQLGHQGPLYIVVMLSLKLA c g t t g c a g g c c a t g c t g t c c a g g c a g g t a g a t g a c g a c c a t c a g g g a c a g c t t c a a g g c c g c a a c g t c c g g t a c g a c a g g t c c g t c c a t c t a c t g c t g g t a g t c c c t g t c g a a g t t c c g g

6060 ori QQKARNRKKAALLAFFHRLR LLLGPVTFLGRQQRKEMPEA a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g

DrdI 6120 ori PPDEHHKNRRSSQRWRNPTG GRVLM c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g

6180 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F L* t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g

6240 ori t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t

6300 ori g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g

6360 ori a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t

6420 ori a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c

6480 ori c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t

6540 ori g a a g g a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g c t t c c t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c

6600 ori g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g

6660 ori a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a

6720 c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t

6780 g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a

6840 285 280 275 *WHKILSAGIEAI AmpR *WHKILSAGIEAI a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t

AhdI 6900 270 265 260 255 QRNREDMTAQSGTTYIVVIR AmpR QRNREDMTAQSGTTYIVVIR t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g

6960 250 245 240 235 SPKGDPGLAAIIGRSGREGA AmpR SPKGDPGLAAIIGRSGREGA g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c

7020 230 225 220 215 GSKDAIFWGAPLASRLLPGA AmpR GSKDAIFWGAPLASRLLPGA c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c

7080 210 205 200 195 VKDAEMWDILQQRSALTLLE AmpR VKDAEMWDILQQRSALTLLE c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a

7140 190 185 180 175 GTLLKRLTTAMAAPMTTDRE AmpR GTLLKRLTTAMAAPMTTDRE c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t g c a g g c a t c g t g g t g t c a c g c t g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a c g t c c g t a g c a c c a c a g t g c g a

7200 170 165 160 155 DNPIAENLEPEWRDLRTVHD AmpR DNPIAENLEPEWRDLRTVHD c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a

7260 150 145 140 135 GMNHLFATLEKPGGITTLLL AmpR GMNHLFATLEKPGGITTLLL c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t

7320 130 125 120 115 NAATNDSMTIAASCLERVTM AmpR NAATNDSMTIAASCLERVTM a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t

ScaI 7380 110 105 100 95 GDTLHKETVPSYEVLDNQSY AmpR GDTLHKETVPSYEVLDNQSY t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a

7440 90 85 80 75 HIRRGLQEQGADVRSLVAGC AmpR A T A A T A C C G C G C C A C Amp-R HIRRGLQEQGADVRSLVAGC a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a c a c g g g a t a a t a c c g c g c c a c t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t g t g c c c t a t t a t g g c g c g g t g

7500 70 65 60 55 LLVKFTSMMPFREEPRFSEL AmpR A T A G C Amp-R LLVKFTSMMPFREEPRFSEL a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t

7560 50 45 40 35 IKGSNLDLEIYGVRAGLQDE AmpR IKGSNLDLEIYGVRAGLQDE g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a

7620 30 25 ADKVKVLTEPH 20 15 signal sequence AFVPLCFAA AmpR ADKVKVLTEPHAFVPLCFAA c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c

7680 10 5 1 signal sequence FFPILAVRFHQISM AmpR AmpR promoter FFPILAVRFHQISM c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a

7740 AmpR promoter a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a

7800 AmpR promoter a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a

7860 A A T A G G C G T A T C A C G A G G C pBRforEco a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g

3ʹ 5ʹ 7862 g t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.