Addgene pAAV-hSyn-GRAB_NTS1.0 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAAV-hSyn-GRAB_NTS1.0 Sequencing Result


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pBR322ori-F (6451 .. 6470) AseI (5543) PvuI (5349) ScaI (5237) Amp-R (5149 .. 5168) XmnI (5118) pBRforEco (4740 .. 4758) pGEX 3' (4680 .. 4702) pRS-marker (4561 .. 4580) PsiI (4447) F1ori-F (4386 .. 4407) F1ori-R (4176 .. 4195) BlpI (3685) NsiI (3676) SphI (3674) hGH-PA-R (3557 .. 3576) BglII (3350) PciI (6606) FspAI (276) attB1 BmgBI (844) BspQI - SapI (902) BfuAI - BspMI (1023) PasI (1077) HincII (1396) BstBI (1751) EGFP-C (1780 .. 1801) Acc65I (1847) KpnI (1851) EGFP-N (1907 .. 1928) EXFP-R (2168 .. 2187) SfiI (2591) EcoRI (2714) EcoRV (2722) HindIII (2726) WPRE-R (2791 .. 2811) PflMI (2979) pAAV-hSyn-GRAB_NTS1.0 6611 bp

Sequence View

60 AAV2 ITR (alternate) c c t g c a g g c a g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c g t c g g g c g a c c t t t g g a c g t c c g t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g c a g c c c g c t g g a a a

120 AAV2 ITR (alternate) g g t c g c c c g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t c c a g c g g g c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a

180 AAV2 ITR (alternate) MRRGGGAYLTTD a g g g g t t c c t g c g g c c g c a c g c g t a t g a g g c g g g g t g g g g g t g c c t a c c t g a c g a c c g a c t c c c c a a g g a c g c c g g c g t g c g c a t a c t c c g c c c c a c c c c c a c g g a t g g a c t g c t g g c t g

240 PDPLDKHPTPIPQIAHPLSE c c c g a c c c a c t g g a c a a g c a c c c a a c c c c c a t t c c c c a a a t t g c g c a t c c c c t a t c a g a g g g g c t g g g t g a c c t g t t c g t g g g t t g g g g g t a a g g g g t t t a a c g c g t a g g g g a t a g t c t c

FspAI 300 RGRGNRMRRGACALPASAPR a g g g g g a g g g g a a a c a g g a t g c g g c g a g g c g c g t g c g c a c t g c c a g c t t c a g c a c c g c g g t c c c c c t c c c c t t t g t c c t a c g c c g c t c c g c g c a c g c g t g a c g g t c g a a g t c g t g g c g c c

360 TVPSPPPGGARHRRLSTEGA a c a g t g c c t t c g c c c c c g c c t g g c g g c g c g c g c c a c c g c c g c c t c a g c a c t g a a g g c g c g t g t c a c g g a a g c g g g g g c g g a c c g c c g c g c g c g g t g g c g g c g g a g t c g t g a c t t c c g c g c

420 LTSLAGPPQTPLPGHLGRVR c t g a c g t c a c t c g c c g g t c c c c c g c a a a c t c c c c t t c c c g g c c a c c t t g g t c g c g t c c g c g a c t g c a g t g a g c g g c c a g g g g g c g t t t g a g g g g a a g g g c c g g t g g a a c c a g c g c a g g c g

480 AAAGPAGPHHARREIGGHGR g c c g c c g c c g g c c c a g c c g g a c c g c a c c a c g c g a g g c g c g a g a t a g g g g g g c a c g g g c g c c g g c g g c g g c c g g g t c g g c c t g g c g t g g t g c g c t c c g c g c t c t a t c c c c c c g t g c c c g c g

540 DHLRCGAGDSALPQSAVGSG g a c c a t c t g c g c t g c g g c g c c g g c g a c t c a g c g c t g c c t c a g t c t g c g g t g g g c a g c g g a c t g g t a g a c g c g a c g c c g c g g c c g c t g a g t c g c g a c g g a g t c a g a c g c c a c c c g t c g c c t

600 1 5 METDT Ig κ leader Kozak sequence GVVSCLRAQSRNRCHHGDRH METDT g g a g t c g t g t c g t g c c t g a g a g c g c a g t c g a g a a a c c g g t g c c a c c a t g g a g a c a g a c a c c c t c a g c a c a g c a c g g a c t c t c g c g t c a g c t c t t t g g c c a c g g t g g t a c c t c t g t c t g t g

660 10 15 20 LLLWVLLLWVPGSTGD TSLY Ig κ leader attB1 TPAMGTAALGSRFHW* LLLWVLLLWVPGSTGDTSLY a c t c c t g c t a t g g g t a c t g c t g c t c t g g g t t c c a g g t t c c a c t g g t g a c a c a a g t t t g t a t g a g g a c g a t a c c c a t g a c g a c g a g a c c c a a g g t c c a a g g t g a c c a c t g t g t t c a a a c a t

720 (in frame with Ig κ leader) KKVGTTGRLNSSAPGTPGTP attB1 KKVGTTGRLNSSAPGTPGTP c a a a a a a g t t g g c a c c a c c g g t c g c c t c a a c a g c t c c g c g c c g g g a a c c c c g g g c a c g c c g t t t t t t c a a c c g t g g t g g c c a g c g g a g t t g t c g a g g c g c g g c c c t t g g g g c c c g t g c g g

780 (in frame with Ig κ leader) AADPFQRAQAGLEEALLAPG AADPFQRAQAGLEEALLAPG g g c c g c c g a c c c c t t c c a g c g g g c g c a g g c c g g a c t g g a g g a g g c g c t g c t g g c c c c g g g c c g g c g g c t g g g g a a g g t c g c c c g c g t c c g g c c t g a c c t c c t c c g c g a c g a c c g g g g c c c

840 (in frame with Ig κ leader) FGNASGNASERVLAAPSSEL FGNASGNASERVLAAPSSEL c t t c g g c a a c g c t t c g g g c a a c g c g t c g g a g c g c g t c c t g g c g g c a c c c a g c a g c g a g c t g a a g c c g t t g c g a a g c c c g t t g c g c a g c c t c g c g c a g g a c c g c c g t g g g t c g t c g c t c g a

BmgBI 900 (in frame with Ig κ leader) DVNTDIYSKVLVTAVYLALF DVNTDIYSKVLVTAVYLALF g g a c g t g a a c a c c g a c a t c t a c t c c a a g g t g c t g g t g a c c g c c g t g t a c c t g g c g c t c t t c c t g c a c t t g t g g c t g t a g a t g a g g t t c c a c g a c c a c t g g c g g c a c a t g g a c c g c g a g a a

BspQISapI 960 (in frame with Ig κ leader) VVGTVGNTVTAFTLARKKSL VVGTVGNTVTAFTLARKKSL c g t g g t g g g c a c g g t g g g c a a c a c g g t g a c g g c g t t c a c g c t g g c g c g g a a g a a g t c g c t g c a c c a c c c g t g c c a c c c g t t g t g c c a c t g c c g c a a g t g c g a c c g c g c c t t c t t c a g c g a

1020 (in frame with Ig κ leader) QSLQSTVHYHLGSLALSDLL QSLQSTVHYHLGSLALSDLL g c a g a g c c t g c a g a g c a c g g t g c a t t a c c a c c t g g g c a g c c t g g c g c t g t c c g a c c t g c t c g t c t c g g a c g t c t c g t g c c a c g t a a t g g t g g a c c c g t c g g a c c g c g a c a g g c t g g a c g a

PasIBspMIBfuAI 1080 (in frame with Ig κ leader) TLLLAMPVELYNFIWVHHPW TLLLAMPVELYNFIWVHHPW c a c c c t g c t g c t g g c c a t g c c c g t g g a g c t g t a c a a c t t c a t c t g g g t g c a c c a c c c c t g g t g g g a c g a c g a c c g g t a c g g g c a c c t c g a c a t g t t g a a g t a g a c c c a c g t g g t g g g g a c

1140 (in frame with Ig κ leader) AFGDAGCRGYYFLRDACTYA AFGDAGCRGYYFLRDACTYA g g c c t t c g g c g a c g c c g g c t g c c g c g g c t a c t a c t t c c t g c g c g a c g c c t g c a c c t a c g c c c g g a a g c c g c t g c g g c c g a c g g c g c c g a t g a t g a a g g a c g c g c t g c g g a c g t g g a t g c g

1200 (in frame with Ig κ leader) TALNVASLSVERYLAICHPF TALNVASLSVERYLAICHPF c a c g g c c c t c a a c g t g g c c a g c c t g a g t g t g g a g c g c t a c c t g g c c a t c t g c c a c c c c t t g t g c c g g g a g t t g c a c c g g t c g g a c t c a c a c c t c g c g a t g g a c c g g t a g a c g g t g g g g a a

1260 (in frame with Ig κ leader) KAKTLMSRSRTKKFISAIWL KAKTLMSRSRTKKFISAIWL c a a g g c c a a g a c c c t c a t g t c c c g a a g c c g c a c c a a g a a g t t c a t c a g c g c c a t c t g g c t g t t c c g g t t c t g g g a g t a c a g g g c t t c g g c g t g g t t c t t c a a g t a g t c g c g g t a g a c c g a

1320 (in frame with Ig κ leader) ASALLAVPMLFTMGEQNRSA ASALLAVPMLFTMGEQNRSA c g c c t c g g c c c t g c t g g c g g t g c c t a t g c t g t t c a c c a t g g g c g a g c a g a a c c g c a g c g c g c g g a g c c g g g a c g a c c g c c a c g g a t a c g a c a a g t g g t a c c c g c t c g t c t t g g c g t c g c g

1380 (in frame with Ig κ leader) DGQHAGGLVCTPTIHTATVK DGQHAGGLVCTPTIHTATVK c g a c g g c c a g c a c g c c g g c g g c c t g g t g t g c a c c c c c a c c a t c c a c a c t g c c a c c g t c a a g c t g c c g g t c g t g c g g c c g c c g g a c c a c a c g t g g g g g t g g t a g g t g t g a c g g t g g c a g t t

HincII 1440 (in frame with Ig κ leader) VVIQVNTFMSFIFPMVVISV VVIQVNTFMSFIFPMVVISV g g t c g t c a t a c a g g t c a a c a c c t t c a t g t c c t t c a t a t t c c c c a t g g t g g t c a t c t c g g t c c a g c a g t a t g t c c a g t t g t g g a a g t a c a g g a a g t a t a a g g g g t a c c a c c a g t a g a g c c a

1500 (in frame with Ig κ leader) LNTIIANKLTVMVRQAAEQL LNTIIANKLTVMVRQAAEQL c c t g a a c a c c a t c a t c g c c a a c a a g c t g a c c g t c a t g g t a c g c c a g g c g g c c g a g c a g c t g g a c t t g t g g t a g t a g c g g t t g t t c g a c t g g c a g t a c c a t g c g g t c c g c c g g c t c g t c g a

1560 (in frame with Ig κ leader) NGAPGEPAPAGPRDTDALDL NGAPGEPAPAGPRDTDALDL c a a c g g c g c c c c t g g c g a g c c c g c g c c g g c c g g g c c g c g c g a c a c c g a c g c g c t g g a c c t g t t g c c g c g g g g a c c g c t c g g g c g c g g c c g g c c c g g c g c g c t g t g g c t g c g c g a c c t g g a

1620 (in frame with Ig κ leader) EEGGNVYIKADKQKNGIKAA EEGGNVYIKADKQKNGIKAA g g a g g a g g g a g g a a a c g t c t a t a t c a a g g c c g a c a a g c a g a a g a a c g g c a t c a a g g c g g c c c t c c t c c c t c c t t t g c a g a t a t a g t t c c g g c t g t t c g t c t t c t t g c c g t a g t t c c g c c g

1680 (in frame with Ig κ leader) FNIRHNIEDGGVQLAYHYQQ FNIRHNIEDGGVQLAYHYQQ g t t c a a t a t c c g c c a c a a c a t c g a g g a c g g c g g c g t g c a g c t c g c c t a c c a c t a c c a g c a c a a g t t a t a g g c g g t g t t g t a g c t c c t g c c g c c g c a c g t c g a g c g g a t g g t g a t g g t c g t

1740 (in frame with Ig κ leader) NTPIGDGPVLLPDNHYLSVQ NTPIGDGPVLLPDNHYLSVQ g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c g t g c a c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g c a c g t

BstBI 1800 (in frame with Ig κ leader) SKLSKDPNEKRDHMVLLEFV C A T G G T C C T G C T G G A G T T C G T EGFP-C SKLSKDPNEKRDHMVLLEFV g t c c a a a c t t t c g a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t c a g g t t t g a a a g c t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a

Acc65IKpnI 1860 (in frame with Ig κ leader) TAAGITWGMDELYKGGTGGS G EGFP-C TAAGITWGMDELYKGGTGGS g a c c g c c g c c g g g a t c a c t t g g g g c a t g g a c g a g c t g t a c a a g g g c g g t a c c g g a g g g a g c t g g c g g c g g c c c t a g t g a a c c c c g t a c c t g c t c g a c a t g t t c c c g c c a t g g c c t c c c t c

1920 (in frame with Ig κ leader) MVRKGEELFTGVVPILVELD G A C C A G C T C G A C C T EGFP-N MVRKGEELFTGVVPILVELD c a t g g t g a g a a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a g t a c c a c t c t t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t

1980 (in frame with Ig κ leader) GDVNGHKFSVGGEGEGDATE G C C G C T G C EGFP-N GDVNGHKFSVGGEGEGDATE c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g g g c g g c g a g g g t g a g g g c g a t g c c a c c g a g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c c c g c c g c t c c c a c t c c c g c t a c g g t g g c t

2040 (in frame with Ig κ leader) GKLTLKFICTTGKLPVPWPT GKLTLKFICTTGKLPVPWPT g g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g

2100 (in frame with Ig κ leader) LVTTLTYGVQCFSRYPDHMK LVTTLTYGVQCFSRYPDHMK c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t

2160 (in frame with Ig κ leader) QHDFFKSAMPEGYIQERTIF QHDFFKSAMPEGYIQERTIF g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c a t c c a g g a g c g c a c c a t c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g t a g g t c c t c g c g t g g t a g a a

2220 (in frame with Ig κ leader) FKDDGNYKTRAEVKFEGDTL C T G C T G C C G T T G A T G T T C T G EXFP-R FKDDGNYKTRAEVKFEGDTL c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a

2280 (in frame with Ig κ leader) VNRIELKGIDFKEDGNILGH VNRIELKGIDFKEDGNILGH g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t

2340 (in frame with Ig κ leader) KLEYNTGAAARWRGREPNRV KLEYNTGAAARWRGREPNRV c a a g c t g g a g t a c a a c a c c g g a g c a g c a g c a c g c t g g c g c g g g c g g g a g c c t a a t a g g g t g t t c g a c c t c a t g t t g t g g c c t c g t c g t c g t g c g a c c g c g c c c g c c c t c g g a t t a t c c c a

2400 (in frame with Ig κ leader) QALRHGVRVLRAVVIAFVVC QALRHGVRVLRAVVIAFVVC c c a g g c c c t g c g g c a c g g c g t g c g c g t c c t a c g t g c a g t g g t c a t c g c c t t t g t g g t c t g g g t c c g g g a c g c c g t g c c g c a c g c g c a g g a t g c a c g t c a c c a g t a g c g g a a a c a c c a g a c

2460 (in frame with Ig κ leader) WLPYHVRRLMFCYISDEQWT WLPYHVRRLMFCYISDEQWT c t g g c t g c c c t a c c a c g t g c g g c g c c t c a t g t t c t g c t a c a t c t c g g a t g a g c a g t g g a c g a c c g a c g g g a t g g t g c a c g c c g c g g a g t a c a a g a c g a t g t a g a g c c t a c t c g t c a c c t g

2520 (in frame with Ig κ leader) PFLYDFYHYFYMVTNALFYV PFLYDFYHYFYMVTNALFYV t c c g t t c c t c t a t g a c t t c t a c c a c t a c t t c t a c a t g g t g a c c a a c g c a c t c t t c t a c g t a g g c a a g g a g a t a c t g a a g a t g g t g a t g a a g a t g t a c c a c t g g t t g c g t g a g a a g a t g c a

2580 (in frame with Ig κ leader) SSTINPILYNLVSANFRHIF SSTINPILYNLVSANFRHIF c a g c t c c a c c a t c a a c c c c a t c c t g t a c a a c c t c g t c t c t g c c a a c t t c c g c c a c a t c t t g t c g a g g t g g t a g t t g g g g t a g g a c a t g t t g g a g c a g a g a c g g t t g a a g g c g g t g t a g a a

SfiI 2640 (in frame with Ig κ leader) LATLACLCPVWRRRRKRPAF LATLACLCPVWRRRRKRPAF c c t g g c c a c a c t g g c c t g c c t c t g c c c g g t g t g g c g g c g c a g g a g g a a g a g g c c a g c c t t g g a c c g g t g t g a c c g g a c g g a g a c g g g c c a c a c c g c c g c g t c c t c c t t c t c c g g t c g g a a

2700 (in frame with Ig κ leader) SRKADSVSSNHTLSSNATRE SRKADSVSSNHTLSSNATRE c t c g a g g a a g g c c g a c a g c g t g t c c a g c a a c c a c a c c c t c t c c a g c a a t g c c a c c c g c g a g a g c t c c t t c c g g c t g t c g c a c a g g t c g t t g g t g t g g g a g a g g t c g t t a c g g t g g g c g c t

EcoRIEcoRVHindIII 2760 TLY* WPRE TLY* g a c g c t g t a c t a g g a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a c a a a a t c t g c g a c a t g a t c c t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t g t t t t a

2820 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g

2880 WPRE MPLYHAIASRMAFIFSSL MLLLPVWLSFSPP t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a

2940 WPRE YKSWLLSLYEELWPVVRQRG CINPGCCLFMRSCGPLSGNV g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c

PflMI 3000 WPRE VVCTVFADATPTGWGIATTC AWCALCLLTQPPLVGALPPP c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c

3060 WPRE QLLSGTFAFPLPIATAELIA VSSFPGLSLSPSLLPRRNSS t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g

3120 WPRE ACLARCWTGARLLGTDNSVV PPALPAAGQGLGCWALTIPW c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a

3180 WPRE (in frame with Factor Xa site) *RGKRPQEGINGGPNQ LSGKSSSFPWLLAYVATWIL CCRGNHRPFLGCSPMLPPGF g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t a t g t t g c c a c c t g g a t t c t c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a t a c a a c g g t g g a c c t a a g a

3240 WPRE (in frame with Factor Xa site) APRGEAVDR 1 RGEI Factor Xa site RGTSFCYVPSALNPADLPSR CAGRPSATSLRPSIQRTFLP g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c

3300 WPRE GLLPALRPLPRLRLRPQTSR AACCRLCGLFRVFAFALRRV c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c

BglII 3360 WPRE hGH poly(A) signal ISLWAASPHRYRALLERSTG GSPFGPPPRIDTERCSRDLR g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g a g c g c t g c t c g a g a g a t c t a c g g g c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c t c g c g a c g a g c t c t c t a g a t g c c c

3420 hGH poly(A) signal GIPVTPPQCLSWPWKLPLQC VASL* t g g c a t c c c t g t g a c c c c t c c c c a g t g c c t c t c c t g g c c c t g g a a g t t g c c a c t c c a g t g a c c g t a g g g a c a c t g g g g a g g g g t c a c g g a g a g g a c c g g g a c c t t c a a c g g t g a g g t c a c

3480 hGH poly(A) signal PPALS* c c c a c c a g c c t t g t c c t a a t a a a a t t a a g t t g c a t c a t t t t g t c t g a c t a g g t g t c c t t c g g g t g g t c g g a a c a g g a t t a t t t t a a t t c a a c g t a g t a a a a c a g a c t g a t c c a c a g g a a g

3540 hGH poly(A) signal t a t a a t a t t a t g g g g t g g a g g g g g g t g g t a t g g a g c a a g g g g c a a g t t g g g a a g a c a a c c a t a t t a t a a t a c c c c a c c t c c c c c c a c c a t a c c t c g t t c c c c g t t c a a c c c t t c t g t t g g

3600 hGH poly(A) signal A G A T A A C C C T T G G T T C G A C C hGH-PA-R t g t a g g g c c t g c g g g g t c t a t t g g g a a c c a a g c t g g a g t g c a g t g g c a c a a t c t t g g c t c a c a t c c c g g a c g c c c c a g a t a a c c c t t g g t t c g a c c t c a c g t c a c c g t g t t a g a a c c g a g

3660 hGH poly(A) signal a c t g c a a t c t c c g c c t c c t g g g t t c a a g c g a t t c t c c t g c c t c a g c c t c c c g a g t t g t t g t g a c g t t a g a g g c g g a g g a c c c a a g t t c g c t a a g a g g a c g g a g t c g g a g g g c t c a a c a a c

BlpISphINsiI 3720 hGH poly(A) signal g g a t t c c a g g c a t g c a t g a c c a g g c t c a g c t a a t t t t t g t t t t t t t g g t a g a g a c g g g g t c c t a a g g t c c g t a c g t a c t g g t c c g a g t c g a t t a a a a a c a a a a a a a c c a t c t c t g c c c c a

3780 hGH poly(A) signal t t c a c c a t a t t g g c c a g g c t g g t c t c c a a c t c c t a a t c t c a g g t g a t c t a c c c a c c t t g g a a g t g g t a t a a c c g g t c c g a c c a g a g g t t g a g g a t t a g a g t c c a c t a g a t g g g t g g a a c c

3840 hGH poly(A) signal c c t c c c a a a t t g c t g g g a t t a c a g g c g t g a a c c a c t g c t c c c t t c c c t g t c c t t c t g a t t g g a g g g t t t a a c g a c c c t a a t g t c c g c a c t t g g t g a c g a g g g a a g g g a c a g g a a g a c t a a

3900 AAV2 ITR AAV2 ITR t t g t a g g t a a c c a c g t g c g g a c c g a g c g g c c g c a g g a a c c c c t a g t g a t g g a g t t g g c c a a a c a t c c a t t g g t g c a c g c c t g g c t c g c c g g c g t c c t t g g g g a t c a c t a c c t c a a c c g g t

3960 AAV2 ITR AAV2 ITR c t c c c t c t c t g c g c g c t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c g a g g g a g a g a c g c g c g a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g

4020 AAV2 ITR AAV2 ITR c g g g c t t t g c c c g g g c g g c c t c a g t g a g c g a g c g a g c g c g c a g c t g c c t g c a g g g g c g c c g c c c g a a a c g g g c c c g c c g g a g t c a c t c g c t c g c t c g c g c g t c g a c g g a c g t c c c c g c g g

4080 t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a c g t c a a a g c a a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t g c a g t t t c g t

4140 f1 ori a c c a t a g t a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g t g g t a t c a t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c

4200 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g t g a c c g c t a c a c t t g c c a g c g c c t t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t g c a c t g g c g a t g t g a a c g g t c g c g g a a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a

4260 f1 ori t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a

4320 f1 ori c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t t g g g t g a t g g t t c a c g g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a a c c c a c t a c c a a g t g c

4380 f1 ori t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a

4440 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c t c t a t c t c g g g c t a t t c t t t a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g a g a t a g a g c c c g a t a a g a a a

PsiI 4500 f1 ori t g a t t t a t a a g g g a t t t t g c c g a t t t c g g t c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c a g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t

4560 f1 ori a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t t a t g g t g c a c t c t c a g t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a a t a c c a c g t g a g a g t c

4620 A T G T T A G A C G A G A C T A C G G C pRS-marker t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t

4680 C pGEX 3' c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g

4740 C G G G A G C T G C A T G T G T C A G A G G pGEX 3' C pBRforEco c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c

4800 G G A G C A C T A T G C G G A T A A pBRforEco c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g

4860 AmpR promoter a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t

4920 AmpR promoter t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t

4980 1 5 10 15 signal sequence MSIQHFRVALIPFFAAF AmpR promoter AmpR MSIQHFRVALIPFFAAF a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a

5040 20 signal sequence CLPVFA 25 30 35 HPETLVKVKDAEDQ AmpR CLPVFAHPETLVKVKDAEDQ t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t

5100 40 45 50 55 LGARVGYIELDLNSGKILES AmpR LGARVGYIELDLNSGKILES g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c

XmnI 5160 60 65 70 75 FRPEERFPMMSTFKVLLCGA AmpR C G A T A C A C C G C G Amp-R FRPEERFPMMSTFKVLLCGA t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g

5220 80 85 90 95 VLSRIDAGQEQLGRRIHYSQ AmpR C C A T A A T A Amp-R VLSRIDAGQEQLGRRIHYSQ g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t

ScaI 5280 100 105 110 115 NDLVEYSPVTEKHLTDGMTV AmpR NDLVEYSPVTEKHLTDGMTV g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a

5340 120 125 130 135 RELCSAAITMSDNTAANLLL AmpR RELCSAAITMSDNTAANLLL a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a

PvuI 5400 140 145 150 155 TTIGGPKELTAFLHNMGDHV AmpR TTIGGPKELTAFLHNMGDHV g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a

5460 160 165 170 175 TRLDRWEPELNEAIPNDERD AmpR TRLDRWEPELNEAIPNDERD a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t

5520 180 185 190 195 TTMPVAMATTLRKLLTGELL AmpR TTMPVAMATTLRKLLTGELL c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a

AseI 5580 200 205 210 215 TLASRQQLIDWMEADKVAGP AmpR TLASRQQLIDWMEADKVAGP t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g

5640 220 225 230 235 LLRSALPAGWFIADKSGAGE AmpR LLRSALPAGWFIADKSGAGE a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t

5700 240 245 250 255 RGSRGIIAALGPDGKPSRIV AmpR RGSRGIIAALGPDGKPSRIV g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a

5760 260 265 270 275 VIYTTGSQATMDERNRQIAE AmpR VIYTTGSQATMDERNRQIAE a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t

5820 280 285 IGASLIKHW* AmpR IGASLIKHW* g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a

5880 t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t

5940 t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a

6000 ori a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t

6060 ori a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a

6120 ori t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t

6180 ori g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a

6240 ori a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g

6300 ori a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t

6360 ori g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t

6420 ori a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c

6480 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a

6540 ori c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c

6600 ori c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a

PciI 6611 t g c t c a c a t g t a c g a g t g t a c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.