Addgene pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP Sequencing Result


Map View

attB4 PciI (6710) pBR322ori-F (6555 .. 6574) AseI (5647) PvuI (5453) ScaI (5341) Amp-R (5253 .. 5272) pBRforEco (4844 .. 4862) pGEX 3' (4784 .. 4806) pRS-marker (4665 .. 4684) PsiI (4551) F1ori-F (4490 .. 4511) BsaAI (4423) F1ori-R (4280 .. 4299) BGH-rev (3757 .. 3774) WPRE-R (3192 .. 3212) PspOMI (97) ApaI (101) FspAI (267) BbvCI (334) RsrII (430) MreI - SgrAI (490) AfeI (503) FRT (minimal) PaeR7I - PspXI - XhoI (751) MluI (765) BsiWI (768) EagI - NotI (786) EGFP-C (838 .. 859) EXFP-R (1187 .. 1206) EGFP-N (1446 .. 1467) AflII (1604) BglII (1646) BsaBI (2126) BstBI (2218) PshAI (2294) BsmI (2410) BsaI (2461) HpaI (2493) SalI (2638) PpuMI * (2871) Myc BamHI (2908) AvrII (2912) SpeI (2938) NheI (2978) BmtI (2982) attB2 pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP 6794 bp

Sequence View

5ʹ 3ʹ 60 AAV2 ITR AAV2 ITR g g c c t c a g t g a g c g a g c g a g c g c g c a g a g a g g g a g t g g c c a a c t c c a t c a c t a g g g g t t c c c g g a g t c a c t c g c t c g c t c g c g c g t c t c t c c c t c a c c g g t t g a g g t a g t g a t c c c c a a g

PspOMIApaI 120 AAV2 ITR attB4 hSyn promoter AAV2 ITR c t t c t a g a c a a c t t t g t a t a g a a a a g t t g c t g c a g a g g g c c c t g c g t a t g a g t g c a a g t g g a a g a t c t g t t g a a a c a t a t c t t t t c a a c g a c g t c t c c c g g g a c g c a t a c t c a c g t t c a c

180 hSyn promoter MRRGGGAYLTTDPDP g g t t t t a g g a c c a g g a t g a g g c g g g g t g g g g g t g c c t a c c t g a c g a c c g a c c c c g a c c c a c c a a a a t c c t g g t c c t a c t c c g c c c c a c c c c c a c g g a t g g a c t g c t g g c t g g g g c t g g g t

240 hSyn promoter LDKHPTPIPQIAHPLSERGR c t g g a c a a g c a c c c a a c c c c c a t t c c c c a a a t t g c g c a t c c c c t a t c a g a g a g g g g g a g g g a c c t g t t c g t g g g t t g g g g g t a a g g g g t t t a a c g c g t a g g g g a t a g t c t c t c c c c c t c c

FspAI 300 hSyn promoter GNRMRRGACALPASAPRTVP g g a a a c a g g a t g c g g c g a g g c g c g t g c g c a c t g c c a g c t t c a g c a c c g c g g a c a g t g c c t c c t t t g t c c t a c g c c g c t c c g c g c a c g c g t g a c g g t c g a a g t c g t g g c g c c t g t c a c g g a

BbvCI 360 hSyn promoter SPPPGGARHRRLSTEGALTS t c g c c c c c g c c t g g c g g c g c g c g c c a c c g c c g c c t c a g c a c t g a a g g c g c g c t g a c g t c a a g c g g g g g c g g a c c g c c g c g c g c g g t g g c g g c g g a g t c g t g a c t t c c g c g c g a c t g c a g t

420 hSyn promoter LAGPPQTPLPGHLGRVRAAA c t c g c c g g t c c c c c g c a a a c t c c c c t t c c c g g c c a c c t t g g t c g c g t c c g c g c c g c c g c c g a g c g g c c a g g g g g c g t t t g a g g g g a a g g g c c g g t g g a a c c a g c g c a g g c g c g g c g g c g g

RsrII 480 hSyn promoter GPAGPHHARREIGGHGRDHL g g c c c a g c c g g a c c g c a c c a c g c g a g g c g c g a g a t a g g g g g g c a c g g g c g c g a c c a t c t g c c g g g t c g g c c t g g c g t g g t g c g c t c c g c g c t c t a t c c c c c c g t g c c c g c g c t g g t a g a c

AfeISgrAIMreI 540 hSyn promoter RCGAGDSALPQSAVGSGGVV c g c t g c g g c g c c g g c g a c t c a g c g c t g c c t c a g t c t g c g g t g g g c a g c g g a g g a g t c g t g g c g a c g c c g c g g c c g c t g a g t c g c g a c g g a g t c a g a c g c c a c c c g t c g c c t c c t c a g c a c

600 hSyn promoter lox2272 attB1 SCLRAQQVCTKKQAATITSY t c g t g c c t g a g a g c g c a g c a a g t t t g t a c a a a a a a g c a g g c t g c c a c c a t a a c t t c g t a t a g c a c g g a c t c t c g c g t c g t t c a a a c a t g t t t t t t c g t c c g a c g g t g g t a t t g a a g c a t a

660 lox2272 RILYTKLSGRMVAGL* a g g a t a c t t t a t a c g a a g t t a t c c g g c a g a a t g g t a g c t g g a t t g t a g c t g c t a t t a g c a t c c t a t g a a a t a t g c t t c a a t a g g c c g t c t t a c c a t c g a c c t a a c a t c g a c g a t a a t c g t

720 loxP FRT (minimal) a t a t g a a a c c t c t t a a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g t t a t g g c g c g g a a g t t a t a c t t t g g a g a a t t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c a a t a c c g c g c c t t c a

MluIBsiWIPaeR7IPspXIXhoI 780 FRT (minimal) t c c t a t t c t c t a g a a a g t a t a g g a a c t t c c c t c g a g t a c c g g t c a c g c g t a c g c a a g c g c a g g a t a a g a g a t c t t t c a t a t c c t t g a a g g g a g c t c a t g g c c a g t g c g c a t g c g t t c g c g

EagINotI 840 235 230 225 *KYLEDMGLTIGAATV EGFP * 235 230 225 KYLEDMGLTIGAATV EGFP G T G EGFP-C *KYLEDMGLTIGAATV c t t a g c g g c c g c t t a c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c g g t c a c g a a t c g c c g g c g a a t g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c g c c a g t g

900 220 215 210 205 FELLVMHDRKENPDKSLASQ EGFP 220 215 210 205 FELLVMHDRKENPDKSLASQ EGFP C T T G A G G T C G T C C T G G T A C EGFP-C FELLVMHDRKENPDKSLASQ g a a c t c c a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c t c a g g g c g g a c t g c t t g a g g t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g a g t c c c g c c t g a c

960 200 195 190 185 TSLYHNDPLLVPGDGIPTNQ EGFP 200 195 190 185 TSLYHNDPLLVPGDGIPTNQ EGFP TSLYHNDPLLVPGDGIPTNQ g g t g c t c a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a t g g g g g t g t t c t g c c a c g a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t a c c c c c a c a a g a c

1020 180 175 170 165 QYHDALQVSGDEINHRIKFN EGFP 180 175 170 165 QYHDALQVSGDEINHRIKFN EGFP QYHDALQVSGDEINHRIKFN c t g g t a g t g g t c g g c g a g c t g c a c g c t g c c g t c c t c g a t g t t g t g g c g g a t c t t g a a g t t g a c c a t c a c c a g c c g c t c g a c g t g c g a c g g c a g g a g c t a c a a c a c c g c c t a g a a c t t c a a

1080 160 155 150 145 VKIGNKQKDAMIYVNHSNYN EGFP 160 155 150 145 VKIGNKQKDAMIYVNHSNYN EGFP VKIGNKQKDAMIYVNHSNYN c a c c t t g a t g c c g t t c t t c t g c t t g t c g g c c a t g a t a t a g a c g t t g t g g c t g t t g t a g t t g t g g a a c t a c g g c a a g a a g a c g a a c a g c c g g t a c t a t a t c t g c a a c a c c g a c a a c a t c a a

1140 140 135 130 125 YELKHGLINGDEKFDIGKLE EGFP 140 135 130 125 YELKHGLINGDEKFDIGKLE EGFP YELKHGLINGDEKFDIGKLE g t a c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a t g c c c t t c a g c t c c a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t a c g g g a a g t c g a g

1200 120 115 110 105 IRNVLTDGEFKVEARTKYNG EGFP 120 115 110 105 IRNVLTDGEFKVEARTKYNG EGFP G T C T T G T A G T T G C C EXFP-R IRNVLTDGEFKVEARTKYNG g a t g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g t c t t g t a g t t g c c c t a c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c a g a a c a t c a a c g g

1260 100 95 90 85 DDKFFITREQVYGEPMASKF EGFP 100 95 90 85 DDKFFITREQVYGEPMASKF EGFP G T C G T C EXFP-R DDKFFITREQVYGEPMASKF g t c g t c c t t g a a g a a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a t g g c g g a c t t g a a c a g c a g g a a c t t c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t a c c g c c t g a a c t t

1320 80 75 70 65 FDHQKMHDPYRSFCQVGYTL EGFP 80 75 70 65 FDHQKMHDPYRSFCQVGYTL EGFP FDHQKMHDPYRSFCQVGYTL g a a g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g c t g a a g c a c t g c a c g c c g t a g g t c a g c t t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c g a c t t c g t g a c g t g c g g c a t c c a g t c

1380 60 55 50 45 TTVLTPWPVPLKGTTCIFKL EGFP 60 55 50 45 TTVLTPWPVPLKGTTCIFKL EGFP TTVLTPWPVPLKGTTCIFKL g g t g g t c a c g a g g g t g g g c c a g g g c a c g g g c a g c t t g c c g g t g g t g c a g a t g a a c t t c a g c c a c c a g t g c t c c c a c c c g g t c c c g t g c c c g t c g a a c g g c c a c c a c g t c t a c t t g a a g t c

1440 40 35 30 25 TLKGYTADGEGEGSVSFKHG EGFP 40 35 30 25 TLKGYTADGEGEGSVSFKHG EGFP TLKGYTADGEGEGSVSFKHG g g t c a g c t t g c c g t a g g t g g c a t c g c c c t c g c c c t c g c c g g a c a c g c t g a a c t t g t g g c c c c a g t c g a a c g g c a t c c a c c g t a g c g g g a g c g g g a g c g g c c t g t g c g a c t t g a a c a c c g g

1500 20 15 10 5 NVDGDLEVLIPVVGTFLEEG EGFP 20 15 10 5 NVDGDLEVLIPVVGTFLEEG EGFP C G T C G C C G T C C A G C T C G A C C A G EGFP-N NVDGDLEVLIPVVGTFLEEG g t t t a c g t c g c c g t c c a g c t c g a c c a g g a t g g g c a c c a c c c c g g t g a a c a g c t c c t c g c c c a a a t g c a g c g g c a g g t c g a g c t g g t c c t a c c c g t g g t g g g g c c a c t t g t c g a g g a g c g g

1560 KS 1a V 1 M 15 10 5 PGPNEEVDGAQKLLSF EGFP P2A KS 1a V 1 M EGFP KSVMPGPNEEVDGAQKLLSF c t t g c t c a c c a t a g g c c c g g g g t t t t c t t c a a c a t c t c c t g c t t g c t t t a a c a g a g a g a a g a a c g a g t g g t a t c c g g g c c c c a a a a g a a g t t g t a g a g g a c g a a c g a a a t t g t c t c t c t t

AflII 1620 (in frame with Myc) GSGGSGIESERRLPRPE 1 NTA P2A NTAGSGGSGIESERRLPRPE g t t c g t g g c t c c g c t t c c t c c a c t g c c t a t c t c c g a t t c t c g c c t t a a g g g c c t g g g c t c c a a g c a c c g a g g c g a a g g a g g t g a c g g a t a g a g g c t a a g a g c g g a a t t c c c g g a c c c g a g

BglII 1680 (in frame with Myc) LPVSAQNHLDIGPPSDTSTS LPVSAQNHLDIGPPSDTSTS t a g a g g t a c g c t t g c c t g g t t g t g g a g a t c t a t g c c a g g a g g t g a g t c t g t g c t t g t g c t a t c t c c a t g c g a a c g g a c c a a c a c c t c t a g a t a c g g t c c t c c a c t c a g a c a c g a a c a c g a

1740 (in frame with Myc) EPVGNESDEEEEKSTLAVEN EPVGNESDEEEEKSTLAVEN c t c t g g g a c t c c g t t c t c a c t a t c c t c c t c t t c c t c t t t g c t t g t t a g g g c a a c t t c a t t g a g a c c c t g a g g c a a g a g t g a t a g g a g g a g a a g g a g a a a c g a a c a a t c c c g t t g a a g t a a

1800 (in frame with Myc) EYCFSNANSLIYKKEALDRA EYCFSNANSLIYKKEALDRA t t c a t a g c a a a a t g a a t t t g c a t t t g a a a g g a t g t a t t t c t t c t c t g c t a a g t c t c t g g c a a g t a t c g t t t t a c t t a a a c g t a a a c t t t c c t a c a t a a a g a a g a g a c g a t t c a g a g a c c g

1860 (in frame with Myc) SCLPTNPVEYTKHFRSYDVK SCLPTNPVEYTKHFRSYDVK a c t a c a a a g g g g g g t g t t a g g t a c t t c a t a a g t c t t a t g g a a t c t t g a a t a g t c t a c t t t t g a t g t t t c c c c c c a c a a t c c a t g a a g t a t t c a g a a t a c c t t a g a a c t t a t c a g a t g a a a

1920 (in frame with Myc) YYHKEEFLVPEYRHGWLIEN YYHKEEFLVPEYRHGWLIEN a t a g t a g t g t t t c t c t t c a a a g a g c a c t g g c t c a t a g c g g t g a c c c c a g a g a a t t t c a t t t a t c a t c a c a a a g a g a a g t t t c t c g t g a c c g a g t a t c g c c a c t g g g g t c t c t t a a a g t a a

1980 (in frame with Myc) ALYSSRCQTTMATAEVMGEL ALYSSRCQTTMATAEVMGEL g g c c a g a t a c g a a c t c c g g c a t t g a g t t g t c a t g g c a g t c g c c t c c a c c a t g c c t t c c a g c c g g t c t a t g c t t g a g g c c g t a a c t c a a c a g t a c c g t c a g c g g a g g t g g t a c g g a a g g t c

2040 (in frame with Myc) IVVIEFDANDIDQKSLDYLP IVVIEFDANDIDQKSLDYLP t a t g a c a a c a a t t t c a a a g t c t g c a t t g t c a a t g t c c t g c t t a c t c a a g t c a t a t a a a g g a t a c t g t t g t t a a a g t t t c a g a c g t a a c a g t t a c a g g a c g a a t g a g t t c a g t a t a t t t c c

2100 (in frame with Myc) SDEDIEHVITIPSVLFIRDI SDEDIEHVITIPSVLFIRDI g c t g t c t t c a t c t a t t t c g t g a a c g a t a g t g a t g g g g g a c a c t a g a a a t a t g c g g t c a a t c g a c a g a a g t a g a t a a a g c a c t t g c t a t c a c t a c c c c c t g t g a t c t t t a t a c g c c a g t t a

BsaBI 2160 (in frame with Myc) GSDFGVNIDIQDLPIFEGES GSDFGVNIDIQDLPIFEGES t c c a c t a t c a a a a c c a a c a t t g a t g t c t a t c t g g t c c a a a g g g a t a a a c t c c c c t t c t g a a g g t g a t a g t t t t g g t t g t a a c t a c a g a t a g a c c a g g t t t c c c t a t t t g a g g g g a a g a c t

BstBI 2220 (in frame with Myc) TIRSKLLQARVHAGVLHSKR TIRSKLLQARVHAGVLHSKR a g t g a t c c t a g a t t t g a g a a g c t g t g c c c g g a c a t g a g c t c c c a c a a g g t g g c t c t t t c g t c a c t a g g a t c t a a a c t c t t c g a c a c g g g c c t g t a c t c g a g g g t g t t c c a c c g a g a a a g c

2280 (in frame with Myc) LNGVRWMLCLKGDRMAIVAN LNGVRWMLCLKGDRMAIVAN a a g g t t a c c c a c t c t c c a c a t c a a g c a g a g t t t g c c a t c c c t c a t g g c a a t c a c a g c a t t t t c c a a t g g g t g a g a g g t g t a g t t c g t c t c a a a c g g t a g g g a g t a c c g t t a g t g t c g t a a

PshAI 2340 (in frame with Myc) HSFVLTENRKKPKAMKAMVA HSFVLTENRKKPKAMKAMVA g t g a c t g a a g a c a a g a g t c t c a t t t c t c t t c t t t g g c t t t g c c a t c t t c g c c a t g a c t g c c a c t g a c t t c t g t t c t c a g a g t a a a g a g a a g a a a c c g a a a c g g t a g a a g c g g t a c t g a c g

2400 (in frame with Myc) GIIFADIICGVISQFVVMFV GIIFADIICGVISQFVVMFV a c c a a t g a t g a a g g c g t c a a t g a t g c a g c c t a c a a t t g a c t g g a a t a c c a c c a t g a a g a c t g g t t a c t a c t t c c g c a g t t a c t a c g t c g g a t g t t a a c t g a c c t t a t g g t g g t a c t t c t g

BsmI 2460 (in frame with Myc) AIPCEDTVCRFAAAITTQTE AIPCEDTVCRFAAAITTQTE a g c a a t t g g g c a t t c g t c t g t c a c a c a c c t g a a a g c a g c g g c a a t g g t t g t c t g g g t c t c t c g t t a a c c c g t a a g c a g a c a g t g t g t g g a c t t t c g t c g c c g t t a c c a a c a g a c c c a g a g

HpaIBsaI 2520 (in frame with Myc) ISFLFAATFSNVESVCAKSV ISFLFAATFSNVESVCAKSV g a t g g a g a a g a g g a a g g c a g c c g t g a a g c t g t t a a c c t c c g a c a c g c a t g c t t t g c t c a c c t a c c t c t t c t c c t t c c g t c g g c a c t t c g a c a a t t g g a g g c t g t g c g t a c g a a a c g a g t g

2580 (in frame with Myc) KSTDLDGHLLAILWFVCGFF KSTDLDGHLLAILWFVCGFF t t t a g a a g t a t c t a g a t c c c c a t g g a g c a g g g c t a t c a a c c a a a a c a c a c a g c c a a a g a a a a a t c t t c a t a g a t c t a g g g g t a c c t c g t c c c g a t a g t t g g t t t t g t g t g t c g g t t t c t t

SalI 2640 (in frame with Myc) LWSLVFALCFIVLMWRWRID LWSLVFALCFIVLMWRWRID c a g c c a g g a g a g c a c g a a g g c a a g a c a g a a g a t a a c c a g c a t c c a c c t c c a g c g g a t g t c g t c g g t c c t c t c g t g c t t c c g t t c t g t c t t c t a t t g g t c g t a g g t g g a g g t c g c c t a c a g

2700 (in frame with Myc) VCTTFIDALYRQGKEGVNIF VCTTFIDALYRQGKEGVNIF g a c a c a g g t a g t a a a g a t g t c t g c c a g g t a c c t c t g t c c c t t c t c a c c c a c g t t g a t a a a c t g t g t c c a t c a t t t c t a c a g a c g g t c c a t g g a g a c a g g g a a g a g t g g g t g c a a c t a t t t

2760 (in frame with Myc) QVNCHGDKKVFRSRCQQRTH QVNCHGDKKVFRSRCQQRTH c t g a a c a t t g c a a t g a c c a t c t t t c t t c a c a a a g c g g c t c c t g c a c t g t t g t c g g g t a t g g a c t t g t a a c g t t a c t g g t a g a a a g a a g t g t t t c g c c g a g g a c g t g a c a a c a g c c c a t a c

2820 (in frame with Myc) VKSKGNGFGNAVAMTALKMG VKSKGNGFGNAVAMTALKMG g a c t t t a c t c t t g c c a t t c c c a a a g c c a t t g g c a a c t g c c a t a g t g g c c a g c t t c a t g c c c t g a a a t g a g a a c g g t a a g g g t t t c g g t a a c c g t t g a c g g t a t c a c c g g t c g a a g t a c g g

PpuMI* 2880 (in frame with Myc) DEESSVISYRNTRVSG 10 LDEE Myc DEESSVISYRNTRVSGLDEE a t c t t c c t c c g a a g a g a c a a t g c t g t a c c g g t t g g t t c t c a c a c t g c c c a g g t c c t c c t c t a g a a g g a g g c t t c t c t g t t a c g a c a t g g c c a a c c a a g a g t g t g a c g g g t c c a g g a g g a g

SpeIBamHIAvrII 2940 5 1 SILKQE Myc Kozak sequence SILKQEM g c t g a t c a g c t t c t g c t c c a t g g t g g c g g a t c c t a g g t a c c t g a g c t c a a c g a t a g c a c t c g a c t a g t c g a a g a c g a g g t a c c a c c g c c t a g g a t c c a t g g a c t c g a g t t g c t a t c g t g a

BmtINheI 3000 FRT (minimal) lox2272 a g t g a a g t t c c t a t t c t c t a g a a a g t a t a g g a a c t t c g c t a g c c g g a t a a c t t c g t a t a a t c a c t t c a a g g a t a a g a g a t c t t t c a t a t c c t t g a a g c g a t c g g c c t a t t g a a g c a t a t t

3060 lox2272 a g t a t c c t a t a c g a a g t t a t t t g c c t t a a c c c a g a a a t t a t c a c t g t t a t t c t t t a g a a t t c a t a g g a t a t g c t t c a a t a a a c g g a a t t g g g t c t t t a a t a g t g a c a a t a a g a a a t c t t a

3120 loxP attB2 MLYEVIPSFLV g g t g c a a a g a a t a a c t t c g t a t a a t g t a t g c t a t a c g a a g t t a t a c c c a g c t t t c t t g t a c c a c g t t t c t t a t t g a a g c a t a t t a c a t a c g a t a t g c t t c a a t a t g g g t c g a a a g a a c a t

3180 attB2 WPRE QSGNSDNQPLDYKICERLTG c a a a g t g g g a a t t c c g a t a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t g t t t c a c c c t t a a g g c t a t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a

3240 WPRE *HRQI A C A A C G A G G A A A A T G C G A T A C WPRE-R ILNYVAPFTLCGYAALMPLY *HRQI a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a

3300 WPRE (in frame with Factor Xa site) MSNSGTHSENEGGQIFGPQQ HAIASRMAFIFSSLYKSWLL MSNSGTHSENEGGQIFGPQQ c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c

3360 WPRE (in frame with Factor Xa site) RKILLQPGNDPLTAHHASHK SLYEELWPVVRQRGVVCTVF RKILLQPGNDPLTAHHASHK t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a

3420 WPRE (in frame with Factor Xa site) SVCGGSTPANGGGTLEKGPS ADATPTGWGIATTCQLLSGT SVCGGSTPANGGGTLEKGPS g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a

3480 WPRE (in frame with Factor Xa site) ESEGERNGRRFEDGGAKGAA FAFPLPIATAELIAACLARC ESEGERNGRRFEDGGAKGAA t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g

3540 WPRE (in frame with Factor Xa site) PCPSPQQASVIGHHQRPLQR WTGARLLGTDNSVVLSGKLT PCPSPQQASVIGHHQRPLQR t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c

3600 WPRE (in frame with Factor Xa site) GKWPQEGTNGGPNQAPRGEA SFPWLLACVATWILRGTSFC GKWPQEGTNGGPNQAPRGEA t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g

3660 WPRE VDR 1 RGEI Factor Xa site YVPSALNPADLPSRGLLPAL VDRRGEIWRVKRGAAQQRSQ t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c

3720 WPRE RPLPRLRLRPQTSRISLWAA PRKRTKAKARLRTPDGKPGG c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g

3780 WPRE bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev SPHREFLELADQPRLCLLVA GRM t c c c c g c a t c g g g a a t t c c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g g g g c g t a g c c c t t a a g g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g

3840 bGH poly(A) signal SHLLFAPPPCLP* a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t

3900 bGH poly(A) signal c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t

3960 bGH poly(A) signal t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a g a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t c t t a t c g t c c g

4020 bGH poly(A) signal AAV2 ITR AAV2 ITR a t g c t g g g g a g g g c c g c a g g a a c c c c t a g t g a t g g a g t t g g c c a c t c c c t c t c t g c g c g c t a c g a c c c c t c c c g g c g t c c t t g g g g a t c a c t a c c t c a a c c g g t g a g g g a g a g a c g c g c g

4080 AAV2 ITR AAV2 ITR t c g c t c g c t c a c t g a g g c c g g g c g a c c a a a g g t c g c c c g a c g c c c g g g c t t t g c c c g g g c a g c g a g c g a g t g a c t c c g g c c c g c t g g t t t c c a g c g g g c t g c g g g c c c g a a a c g g g c c c g

4140 AAV2 ITR AAV2 ITR g g c c t c a g t g a g c g a g c g a g c g c g c a g c t g c c t g c a g g g g c g c c t g a t g c g g t a t t t t c t c c g g a g t c a c t c g c t c g c t c g c g c g t c g a c g g a c g t c c c c g c g g a c t a c g c c a t a a a a g a

4200 f1 ori c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a c g t c a a a g c a a c c a t a g t a c g c g c c c g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t g c a g t t t c g t t g g t a t c a t g c g c g g g

4260 f1 ori t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a

4320 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R g c c a g c g c c t t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c c g g t c g c g g a a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g

4380 f1 ori g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t

BsaAI 4440 f1 ori c g g c a c c t c g a c c c c a a a a a a c t t g a t t t g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c g c c g t g g a g c t g g g g t t t t t t g a a c t a a a c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g

4500 f1 ori G T G G A C T C T T G F1ori-F t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c

PsiI 4560 f1 ori T T C C A A A C T G G F1ori-F t t c c a a a c t g g a a c a a c a c t c a a c t c t a t c t c g g g c t a t t c t t t t g a t t t a t a a g g g a t t a a g g t t t g a c c t t g t t g t g a g t t g a g a t a g a g c c c g a t a a g a a a a c t a a a t a t t c c c t a a

4620 f1 ori t t g c c g a t t t c g g t c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t a a c g g c t a a a g c c a g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a

4680 f1 ori A T G T T A G A C G A G A C T A pRS-marker t t t a a c a a a a t a t t a a c g t t t a c a a t t t t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t a a a t t g t t t t a t a a t t g c a a a t g t t a a a a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a

4740 C G G C pRS-marker g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a

4800 C C G G G A G C T G C A T G T G T pGEX 3' t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a

4860 C A G A G G pGEX 3' C G G A G C A C T A T G C G G A T pBRforEco c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t

4920 A A pBRforEco t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c

4980 AmpR promoter g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c

5040 1 signal se... MS AmpR promoter AmpR MS c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a

5100 5 10 15 20 signal sequence IQHFRVALIPFFAAFCLPVF AmpR IQHFRVALIPFFAAFCLPVF a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a

5160 sig... A 25 30 35 40 HPETLVKVKDAEDQLGARV AmpR AHPETLVKVKDAEDQLGARV g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c

5220 45 50 55 60 GYIELDLNSGKILESFRPEE AmpR GYIELDLNSGKILESFRPEE g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t

5280 65 70 75 80 RFPMMSTFKVLLCGAVLSRI AmpR C G A T A C A C C G C G C C A T A A T A Amp-R RFPMMSTFKVLLCGAVLSRI c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a

5340 85 90 95 100 DAGQEQLGRRIHYSQNDLVE AmpR DAGQEQLGRRIHYSQNDLVE g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c

ScaI 5400 105 110 115 120 YSPVTEKHLTDGMTVRELCS AmpR YSPVTEKHLTDGMTVRELCS t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a

PvuI 5460 125 130 135 140 AAITMSDNTAANLLLTTIGG AmpR AAITMSDNTAANLLLTTIGG g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t

5520 145 150 155 160 PKELTAFLHNMGDHVTRLDR AmpR PKELTAFLHNMGDHVTRLDR c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a

5580 165 170 175 180 WEPELNEAIPNDERDTTMPV AmpR WEPELNEAIPNDERDTTMPV t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t

5640 185 190 195 200 AMATTLRKLLTGELLTLASR AmpR AMATTLRKLLTGELLTLASR g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c

AseI 5700 205 210 215 220 QQLIDWMEADKVAGPLLRSA AmpR QQLIDWMEADKVAGPLLRSA c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g

5760 225 230 235 240 LPAGWFIADKSGAGERGSRG AmpR LPAGWFIADKSGAGERGSRG c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g a a g c c g c g g t g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c t t c g g c g c c a

5820 245 250 255 260 IIAALGPDGKPSRIVVIYTT AmpR IIAALGPDGKPSRIVVIYTT a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c

5880 265 270 275 280 GSQATMDERNRQIAEIGASL AmpR GSQATMDERNRQIAEIGASL g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c

5940 285 IKHW* AmpR IKHW* a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t

6000 c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t

6060 a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t

6120 ori t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c

6180 ori c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a

6240 ori g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g

6300 ori c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c

6360 ori g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c

6420 ori g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t

6480 ori a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g

6540 ori g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c

6600 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g

6660 ori t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g

PciI 6720 AAV2 ITR a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t c c t g c t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a g g a c g

6780 AAV2 ITR AAV2 ITR a g g c a g c t g c g c g c t c g c t c g c t c a c t g a g g c c g c c c g g g c a a a g c c c g g g c g t c g g g c g t c c g t c g a c g c g c g a g c g a g c g a g t g a c t c c g g c g g g c c c g t t t c g g g c c c g c a g c c c g c

3ʹ 5ʹ 6794 AAV2 ITR AAV2 ITR a c c t t t g g t c g c c c t g g a a a c c a g c g g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.