Addgene TFORF3196 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: TFORF3196 Sequencing Result


Map View

PaqCI (9721) PspXI (9720) EcoRI (9519) Puro-F (9411 .. 9431) RsrII (9031) BspEI * (9028) BsiWI (8971) PflFI - Tth111I (8957) Puro-R (8915 .. 8934) KpnI (8900) Acc65I (8896) NsiI (8613) KflI - PpuMI (8603) NotI (7817) NruI * (7506) T3 promoter T3 (6852 .. 6872) M13 rev M13 Reverse (6817 .. 6833) lac operator M13/pUC Reverse (6798 .. 6820) lac promoter CAP binding site L4440 (6585 .. 6602) pBR322ori-F (6332 .. 6351) AhdI (5599) ScaI (5118) Amp-R (5030 .. 5049) SbfI (9732) AgeI (79) EF1a-F (1127 .. 1147) NheI (1181) BmtI (1185) BspDI - ClaI (1188) BclI * (1193) BstXI (1231) MreI - SgrAI (1853) XcmI (2086) PmlI (2287) attB2 EcoRV (2596) SpeI (2651) WPRE-R (2805 .. 2825) PflMI (2993) SV40pA-R (3760 .. 3779) EBV-rev (3814 .. 3833) T7 promoter T7 (4039 .. 4058) M13 fwd M13 Forward (4068 .. 4085) M13/pUC Forward (4077 .. 4099) F1ori-R (4313 .. 4332) F1ori-F (4523 .. 4544) TFORF3196 9814 bp

Sequence View

60 EF-1 α promoter g g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g c c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c

AgeI 120 EF-1 α promoter g g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t c c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a

180 EF-1 α promoter g a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a c t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t

240 EF-1 α promoter EF-1 α intron A g t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c c a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g

300 EF-1 α promoter EF-1 α intron A * g t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t c a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a

360 EF-1 α promoter EF-1 α intron A KWRAATRSEQDRAEPQFHTP a c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g t g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c

420 EF-1 α promoter EF-1 α intron A SNSAKRKLLGKAEHKLQPRA g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c

480 EF-1 α promoter EF-1 α intron A QASPGGRAFRTAGERRDRQK c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a

540 EF-1 α promoter EF-1 α intron A RYTELWKFNKIVQQSAKKRA t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c

600 EF-1 α promoter EF-1 α intron A LYDQLHPGLDACQYKPKQPR c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g

660 EF-1 α promoter EF-1 α intron A PRRRPGHTGACMNPSAPGAL g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t

720 EF-1 α promoter EF-1 α intron A AAVSFRVPTTELQGAQEPAQ g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a

780 EF-1 α promoter EF-1 α intron A GRAATYRGARPPLAPGTPVL g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t

840 EF-1 α promoter EF-1 α intron A QTLPFIAAERGQQLSSLISS g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c

900 EF-1 α promoter EF-1 α intron A AASPLAPPHTVWVFSFPRET a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c

960 EF-1 α promoter EF-1 α intron A RLRRKM t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a

1020 EF-1 α promoter EF-1 α intron A t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c

1080 EF-1 α promoter EF-1 α intron A g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t

1140 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A EF1a-F t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t

BclI*NheIBmtIBspDIClaI 1200 EF-1 α promoter attB1 EF-1 α intron A G T G G T T C EF1a-F *C g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g c t a g c a t c g a t t g a t c a a c c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c g a t c g t a g c t a a c t a g t t g

BstXI 1260 attB1 MLLDAGPQYPAI TQVFFNAGHEQVGAGLVWRD a a g t t t g t a c a a a a a a g t t g g c a c c a t g c t c c t g g a c g c c g g c c c c c a g t a c c c a g c g a t t t c a a a c a t g t t t t t t c a a c c g t g g t a c g a g g a c c t g c g g c c g g g g g t c a t g g g t c g c t a

1320 GVTTFGASRHHSAGDVAERD AHGGKARGAVVGRAVHGFSV c g g c g t g a c c a c c t t t g g c g c g t c c c g c c a c c a c t c c g c g g g c g a c g t g g c c g a a c g a g a g c c g c a c t g g t g g a a a c c g c g c a g g g c g g t g g t g a g g c g c c c g c t g c a c c g g c t t g c t c t

1380 VGLGINPFADGMGAFKLNPS HAQADVREGVAHAGELEVGT c g t g g g c c t g g g c a t c a a c c c g t t c g c c g a c g g c a t g g g c g c c t t c a a g c t c a a c c c c a g g c a c c c g g a c c c g t a g t t g g g c a a g c g g c t g c c g t a c c c g c g g a a g t t c g a g t t g g g g t c

1440 SHELASAGQTAFTSQAPGYA RVLQSRGALRGERRLRWAVR t t c g c a c g a g c t g g c t t c g g c c g g c c a g a c g g c c t t c a c g t c g c a g g c g c c a g g c t a c g c a a g c g t g c t c g a c c g a a g c c g g c c g g t c t g c c g g a a g t g c a g c g t c c g c g g t c c g a t g c g

1500 AAAALGHHHHPGHVGSYSSA SSRGQAMVMVRAVDAGIGAC g g c t g c t g c g g c c c t g g g c c a t c a c c a t c a c c c g g g c c a c g t c g g c t c c t a t t c c a g c g c c c g a c g a c g c c g g g a c c c g g t a g t g g t a g t g g g c c c g g t g c a g c c g a g g a t a a g g t c g c g

1560 AFNSTRDFLFRNRGFGDAAA GEVGRPVKQEAVPTKAVRRR a g c c t t c a a c t c c a c g c g g g a c t t t c t g t t c c g c a a c c g g g g t t t t g g c g a c g c g g c g g c t c g g a a g t t g a g g t g c g c c c t g a a a g a c a a g g c g t t g g c c c c a a a a c c g c t g c g c c g c c g

1620 AASAQHSLFAASAGGFGGPH CGACLVAEKSCRGPAEPAWV g g c a g c c a g c g c a c a g c a c a g c c t c t t t g c t g c a t c g g c c g g g g g c t t c g g g g g c c c a c a c c g t c g g t c g c g t g t c g t g t c g g a g a a a c g a c g t a g c c g g c c c c c g a a g c c c c c g g g t g t

1680 GHTDAAGHLLFPGLHEQAAG AVRVGRAVEEEGPKVLLSGA c g g c c a c a c g g a c g c c g c g g g c c a c c t c c t c t t c c c c g g g c t t c a c g a g c a g g c t g c c g g g c c g g t g t g c c t g c g g c g c c c g g t g g a g g a g a a g g g g c c c g a a g t g c t c g t c c g a c g g c c

1740 HASPNVVNGQMRLGFSGDMY VRRRVHDVPLHPEAERPVHV c c a c g c g t c g c c t a a c g t g g t c a a c g g g c a g a t g a g g c t c g g c t t c t c g g g g g a c a t g t a g g t g c g c a g c g g a t t g c a c c a g t t g c c c g t c t a c t c c g a g c c g a a g a g c c c c c t g t a c a t

1800 PRPEQYGQVTSPRSEHYAAP RSRLLVALHGARTRLVISRR c c c g c g a c c g g a g c a g t a c g g c c a g g t g a c c a g c c c g c g t t c g g a g c a c t a t g c t g c g c c g g g c g c t g g c c t c g t c a t g c c g g t c c a c t g g t c g g g c g c a a g c c t c g t g a t a c g a c g c g g

SgrAIMreI 1860 QLHGYGPMNVNMAAHHGAGA LQVAVPGHVHVHGRM g c a g c t g c a c g g c t a c g g g c c c a t g a a c g t g a a c a t g g c c g c g c a t c a c g g c g c c g g c g c c g t c g a c g t g c c g a t g c c c g g g t a c t t g c a c t t g t a c c g g c g c g t a g t g c c g c g g c c g c g

1920 FFRYMRQPIKQELICKWIEP c t t c t t c c g c t a c a t g c g c c a a c c c a t c a a g c a a g a g c t c a t c t g c a a g t g g a t c g a g c c g a a g a a g g c g a t g t a c g c g g t t g g g t a g t t c g t t c t c g a g t a g a c g t t c a c c t a g c t c g g

1980 EQLANPKKSCNKTFSTMHEL c g a g c a g c t g g c c a a c c c c a a a a a g t c g t g c a a c a a a a c t t t c a g c a c c a t g c a c g a g c t g c t c g t c g a c c g g t t g g g g t t t t t c a g c a c g t t g t t t t g a a a g t c g t g g t a c g t g c t c g a

2040 VTHVTVEHVGGPEQSNHICF a g t t a c g c a c g t c a c c g t g g a g c a c g t a g g t g g c c c g g a g c a g a g t a a t c a c a t c t g c t t t c a a t g c g t g c a g t g g c a c c t c g t g c a t c c a c c g g g c c t c g t c t c a t t a g t g t a g a c g a a

XcmI 2100 WEECPREGKPFKAKYKLVNH c t g g g a g g a g t g t c c g c g c g a g g g c a a g c c c t t c a a a g c c a a a t a c a a a c t g g t t a a c c a g a c c c t c c t c a c a g g c g c g c t c c c g t t c g g g a a g t t t c g g t t t a t g t t t g a c c a a t t g g t

2160 IRVHTGEKPFPCPFPGCGKV c a t c c g c g t g c a c a c g g g c g a g a a g c c c t t t c c c t g c c c c t t c c c t g g c t g t g g c a a g g t g t a g g c g c a c g t g t g c c c g c t c t t c g g g a a a g g g a c g g g g a a g g g a c c g a c a c c g t t c c a

2220 FARSENLKIHKRTHTGEKPF c t t c g c g c g c t c c g a g a a t t t a a a g a t c c a c a a a a g g a c g c a c a c a g g g g a g a a g c c c t t g a a g c g c g c g a g g c t c t t a a a t t t c t a g g t g t t t t c c t g c g t g t g t c c c c t c t t c g g g a a

2280 KCEFEGCDRRFANSSDRKKH c a a g t g c g a g t t t g a g g g c t g t g a c c g g c g c t t c g c t a a c a g c a g c g a c c g c a a g a a g c a g t t c a c g c t c a a a c t c c c g a c a c t g g c c g c g a a g c g a t t g t c g t c g c t g g c g t t c t t c g t

PmlI 2340 MHVHTSDKPYLCKMCDKSYT c a t g c a c g t g c a c a c g a g c g a c a a g c c c t a t c t t t g c a a g a t g t g c g a c a a g t c c t a c a c g t a c g t g c a c g t g t g c t c g c t g t t c g g g a t a g a a a c g t t c t a c a c g c t g t t c a g g a t g t g

2400 HPSSLRKHMKVHESSSQGSQ g c a t c c c a g t t c g c t g c g c a a a c a c a t g a a g g t c c a c g a a t c c t c c t c g c a g g g c t c g c a c g t a g g g t c a a g c g a c g c g t t t g t g t a c t t c c a g g t g c t t a g g a g g a g c g t c c c g a g c g t

2460 PSPAASSGYESSTPPTIVSP g c c t t c g c c g g c c g c c a g c t c t g g c t a c g a a t c c t c c a c g c c t c c c a c c a t c g t g t c t c c c g g a a g c g g c c g g c g g t c g a g a c c g a t g c t t a g g a g g t g c g g a g g g t g g t a g c a c a g a g g

2520 STDNPTTSSLSPSSSAVHHT c t c c a c a g a c a a c c c g a c c a c a a g c t c c t t a t c g c c c t c c t c c t c c g c a g t c c a c c a c a c g a g g t g t c t g t t g g g c t g g t g t t c g a g g a a t a g c g g g a g g a g g a g g c g t c a g g t g g t g t g

2580 attB2 AGHSALSSNFNEWYVLPTFL a g c c g g c c a c a g t g c g c t c t c t t c c a a t t t t a a c g a a t g g t a c g t t t t g c c a a c t t t c t t t c g g c c g g t g t c a c g c g a g a g a a g g t t a a a a t t g c t t a c c a t g c a a a a c g g t t g a a a g a a

EcoRV 2640 1 5 10 GKPIPNPLLGLDST attB2 V5 tag YKVVDIGKPIPNPLLGLDST g t a c a a a g t g g t t g a t a t c g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t c t c g a t t c t a c c a t g t t t c a c c a a c t a t a g c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a g a g c t a a g a t g

SpeI 2700 * V5 tag * g t a g t a a t g a a c t a g t c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g c a t c a t t a c t t g a t c a g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c

2760 WPRE t g g a a a g g a c g a g t t t g c a a c c a a t c c c c c t a t t g a a c g c g t t a a g t c g a c a a t c a a c c t a c c t t t c c t g c t c a a a c g t t g g t t a g g g g g a t a a c t t g c g c a a t t c a g c t g t t a g t t g g a

2820 WPRE A C A A C G A G G A A A A T G C WPRE-R c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c

2880 WPRE G A T A C WPRE-R MPLYHAIASRMAF MLLLPVWLS c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g

2940 WPRE IFSSLYKSWLLSLYEELWPV FSPPCINPGCCLFMRSCGPL a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a

PflMI 3000 WPRE VRQRGVVCTVFADATPTGWG SGNVAWCALCLLTQPPLVGA g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g

3060 WPRE IATTCQLLSGTFAFPLPIAT LPPPVSSFPGLSLSPSLLPR a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c

3120 WPRE AELIAACLARCWTGARLLGT RNSSPPALPAAGQGLGCWAL g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a

3180 WPRE (in frame with Factor Xa site) *RGKRPQEGTN DNSVVLSGKSSSFPWLLACV TIPWCCRGNHRPFLGCSPVL g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a

3240 WPRE (in frame with Factor Xa site) GGPNQAPRGEAVDR 1 RGEI Factor Xa site ATWILRGTSFCYVPSALNPA PPGFCAGRPSATSLRPSIQR g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c

3300 WPRE DLPSRGLLPALRPLPRLRLR TFLPAACCRLCGLFRVFAFA g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g

3360 WPRE PQTSRISLWAASPRRL* LRRVGSPFGPPPRVDFKTND c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c g t c g a c t t t a a g a c c a a t g a g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g c a g c t g a a a t t c t g g t t a c t

3420 3' LTR ( Δ U3) LQGSCRS* c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g

3480 3' LTR ( Δ U3) t a a t t c a c t c c c a a c g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a t t a a g t g a g g g t t g c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c

3540 3' LTR ( Δ U3) a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a

3600 3' LTR ( Δ U3) a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a

3660 3' LTR ( Δ U3) a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a c g t a t a g t a g t t t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t g c a t a t c a t c a a

3720 c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t

3780 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R g g a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c c t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t

3840 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a

3900 SV40 poly(A) signal SV40 ori c t t a t c a t g t c t g g c t c t a g c t a t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g a a t a g t a c a g a c c g a g a t c g a t a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g

3960 SV40 ori g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g

4020 SV40 ori c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a

4080 T7 promoter M13 fwd G G G A T A T C A C T C A G C A T A A T T7 T G A C C G G C A G C A A M13 Forward G C A A M13/pUC Forward a g g g a c g t a c c c a a t t c g c c c t a t a g t g a g t c g t a t t a c g c g c g c t c a c t g g c c g t c g t t t c c c t g c a t g g g t t a a g c g g g a t a t c a c t c a g c a t a a t g c g c g c g a g t g a c c g g c a g c a a

4140 M13 fwd A A T G T M13 Forward A A T G T T G C A G C A C T G A C C C M13/pUC Forward t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a

4200 c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c

4260 f1 ori t t g c g c a g c c t g a a t g g c g a a t g g g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t a a c g c g t c g g a c t t a c c g c t t a c c c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a

4320 f1 ori G A G G A A A G F1ori-R g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g

4380 f1 ori C G A A A G A A G G G A F1ori-R g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c

4440 f1 ori g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a

4500 f1 ori t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c

4560 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a

4620 f1 ori a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t

4680 f1 ori a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a

4740 f1 ori AmpR promoter t a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g

4800 AmpR promoter a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t

4860 1 5 10 15 signal sequence MSIQHFRVALIPFFAA AmpR promoter AmpR MSIQHFRVALIPFFAA a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a

4920 20 signal sequence FCLPVFA 25 30 35 HPETLVKVKDAED AmpR FCLPVFAHPETLVKVKDAED t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g

4980 40 45 50 55 QLGARVGYIELDLNSGKILE AmpR QLGARVGYIELDLNSGKILE a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t

5040 60 65 70 75 SFRPEERFPMMSTFKVLLCG AmpR C G A T A C A C C G C Amp-R SFRPEERFPMMSTFKVLLCG g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c

5100 80 85 90 95 AVLSRIDAGQEQLGRRIHYS AmpR G C C A T A A T A Amp-R AVLSRIDAGQEQLGRRIHYS c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g

ScaI 5160 100 105 110 115 QNDLVEYSPVTEKHLTDGMT AmpR QNDLVEYSPVTEKHLTDGMT a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c

5220 120 125 130 135 VRELCSAAITMSDNTAANLL AmpR VRELCSAAITMSDNTAANLL t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g

5280 140 145 150 155 LTTIGGPKELTAFLHNMGDH AmpR LTTIGGPKELTAFLHNMGDH t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c

5340 160 165 170 175 VTRLDRWEPELNEAIPNDER AmpR VTRLDRWEPELNEAIPNDER t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c

5400 180 185 190 195 DTTMPVAMATTLRKLLTGEL AmpR DTTMPVAMATTLRKLLTGEL a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g

5460 200 205 210 215 LTLASRQQLIDWMEADKVAG AmpR LTLASRQQLIDWMEADKVAG t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g

5520 220 225 230 235 PLLRSALPAGWFIADKSGAG AmpR PLLRSALPAGWFIADKSGAG c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c

5580 240 245 250 255 ERGSRGIIAALGPDGKPSRI AmpR ERGSRGIIAALGPDGKPSRI a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c

AhdI 5640 260 265 270 275 VVIYTTGSQATMDERNRQIA AmpR VVIYTTGSQATMDERNRQIA t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c

5700 280 285 EIGASLIKHW* AmpR EIGASLIKHW* a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g

5760 t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c

5820 a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c

5880 ori t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g

5940 ori a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g

6000 ori t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a

6060 ori a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g

6120 ori t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a

6180 ori c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g

6240 ori a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c

6300 ori a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c

6360 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c

6420 ori t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t

6480 ori g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a

6540 t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a

6600 A G C G A G T C A G T G A G C G L4440 t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c

6660 A G L4440 a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a

6720 CAP binding site a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t

6780 -35 CAP binding site lac promoter a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t

6840 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a

6900 T3 promoter RSV promoter G C A A T T A A C C C T C A C T A A A G G T3 a c g c c a a g c g c g c a a t t a a c c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t t a a t t g c g g t t c g c g c g t t a a t t g g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g a a t t a

6960 RSV promoter g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a c a t g c c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t g t a c g

7020 RSV promoter c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g a t c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g c t a g c

7080 RSV promoter t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a t t g c c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t a a c g g

7140 RSV promoter 5' LTR (truncated) g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c t g g t t c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g a c c a a

7200 5' LTR (truncated) a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t

7260 5' LTR (truncated) a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t

7320 5' LTR (truncated) c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g

7380 HIV-1 Ψ a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a

7440 HIV-1 Ψ g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a

7500 HIV-1 Ψ a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a

NruI* 7560 a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t

7620 c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t

7680 a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t

7740 g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t

7800 g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c

NotI 7860 MRDN a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t

7920 WRSELYKYKVVKIEPLGVAP t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g

7980 RRE TKAKRRVVQREKRAVGIGAL c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a

8040 RRE FLGFLGAAGSTMGAASMTLT g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g

8100 RRE VQARQLLSGIVQQQNNLLRA g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g

8160 RRE IEAQQHLLQLTVWGIKQLQA t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g

8220 RRE RILAVERYLKDQQLLGIWGC a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c

8280 SGKLICTTAVPWNASWSNKS c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g

8340 LEQIWNHTTWMEWDREINNY t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t

8400 1 KNEQ gp41 peptide TSLIHSLIEESQNQQEKNEQ c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t

8460 5 10 15 ELLELDKWASL (in frame with gp41 peptide) WNWFNITNW gp41 peptide ELLELDKWASLWNWFNITNW a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c

8520 (in frame with gp41 peptide) LWYIKLFIMIVGGLVGLRIV LWYIKLFIMIVGGLVGLRIV g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a

8580 (in frame with gp41 peptide) FAVLSIVNRVRQGYSPLSFQ FAVLSIVNRVRQGYSPLSFQ t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t

NsiIPpuMIKflI 8640 (in frame with gp41 peptide) THLPTPRGPMHCISISQQPG THLPTPRGPMHCISISQQPG g a c c c a c c t c c c a a c c c c g a g g g g a c c c a t g c a t t g c a t c t c a a t t a g t c a g c a a c c a g g c t g g g t g g a g g g t t g g g g c t c c c c t g g g t a c g t a a c g t a g a g t t a a t c a g t c g t t g g t c c

8700 (in frame with gp41 peptide) VESPQAPQQAEVCKACVSIS SV40 promoter SV40 promoter VESPQAPQQAEVCKACVSIS t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c t c a a t t a g a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g c a g a g t t a a t c

8760 QQP* SV40 ori SV40 promoter SV40 promoter QQP* t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g

8820 SV40 promoter SV40 promoter SV40 ori g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g

8880 SV40 promoter SV40 promoter SV40 ori t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g

Acc65IKpnI 8940 1 5 MTEYKPTVR SV40 promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R MTEYKPTVR a a a a a g c t t t c t a g a g g t a c c a c c g g a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g t t t t t c g a a a g a t c t c c a t g g t g g c c t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c

BsiWITth111IPflFI 9000 10 15 20 25 LATRDDVPRAVRTLAAAFAD PuroR LATRDDVPRAVRTLAAAFAD c c t c g c c a c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a g g a g c g g t g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t

BspEI*RsrII 9060 30 35 40 45 YPATRHTVDPDRHIERVTEL PuroR YPATRHTVDPDRHIERVTEL c t a c c c c g c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a

9120 50 55 60 65 QELFLTRVGLDIGKVWVADD PuroR QELFLTRVGLDIGKVWVADD g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t

9180 70 75 80 85 GAAVAVWTTPESVEAGAVFA PuroR GAAVAVWTTPESVEAGAVFA c g g c g c c g c c g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c g c c g c g g c g g c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g

9240 90 95 100 105 EIGPRMAELSGSRLAAQQQM PuroR EIGPRMAELSGSRLAAQQQM c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a

9300 110 115 120 125 EGLLAPHRPKEPAWFLATVG PuroR EGLLAPHRPKEPAWFLATVG g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c

9360 130 135 140 145 VSPDHQGKGLGSAVVLPGVE PuroR VSPDHQGKGLGSAVVLPGVE c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t

9420 150 155 160 165 AAERAGVPAFLETSAPRNLP PuroR G C A A C C T C C C Puro-F AAERAGVPAFLETSAPRNLP g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g

9480 170 175 180 185 FYERLGFTVTADVEVPEGPR PuroR C T T C T A C G A G C Puro-F FYERLGFTVTADVEVPEGPR c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c

EcoRI 9540 190 195 200 TWCMTRKPGAS* PuroR TWCMTRKPGAS* c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c a g t t a a c g a a t t c t a g a t c t t g a g a c a a a g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g t c a a t t g c t t a a g a t c t a g a a c t c t g t t t

9600 cPPT/CTS t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c

9660 cPPT/CTS a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t

9720 cPPT/CTS c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t g g c g c c g g c g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a a c c g c g g c c g

SbfIPspXIPaqCI 9780 t c g a g g c c t g c a g g t g c a a a g a t g g a t a a a g t t t t a a a c a g a g a g g a a t c t t t g c a g c t a a g c t c c g g a c g t c c a c g t t t c t a c c t a t t t c a a a a t t t g t c t c t c c t t a g a a a c g t c g a t

9814 a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.