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Sequence Analyzer: pU-mWasabi Sequencing Result


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AatII (5627) ZraI (5625) Amp-R (5255 .. 5274) TsoI (5104) FspI (4927) NmeAIII (4853) AhdI (4705) BanI (4653) pBR322ori-F (3953 .. 3972) L4440 (3702 .. 3719) HpaI (3633) T7 (3596 .. 3615) SacII (3581) EagI - NotI (3575) SfiI (3574) SpeI (3561) BspDI - ClaI (3544) EcoRI (3537) SacI (3535) Eco53kI (3533) Bpu10I (3457) StuI (3163) ApaI * (3105) PspOMI * (3101) BsmI (3089) NcoI (2932) XcmI (2855) pRS-marker (139 .. 158) SnaBI (207) MfeI (570) GPDpro-F (713 .. 736) SalI (844) PacI (874) MluI (967) PflMI (1287) BsrGI (1579) AscI - BssHII (1591) pHybLex-R (1665 .. 1689) I-SceI (1815) BglII (1827) BstEII (1857) SwaI (2338) PmlI (2362) BsgI (2673) PstI - SbfI (2709) pU-mWasabi 5704 bp

Sequence View

5ʹ 3ʹ 60 g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a

120 a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g

180 GAP promoter C G G C A T C A G A G C A G A T T G T A pRS-marker g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g t c a t c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c a g t a

SnaBI 240 GAP promoter t a t c a a t a c t g c c a t t t c a a a g a a t a c g t a a a t a a t t a a t a g t a g t g a t t t t c c t a a c t t a t a g t t a t g a c g g t a a a g t t t c t t a t g c a t t t a t t a a t t a t c a t c a c t a a a a g g a t t g a a

300 GAP promoter t a t t t a g t c a a a a a a t t a g c c t t t t a a t t c t g c t g t a a c c c g t a c a t g c c c a a a a t a g g g a t a a a t c a g t t t t t t a a t c g g a a a a t t a a g a c g a c a t t g g g c a t g t a c g g g t t t t a t c c c

360 GAP promoter g g c g g g t t a c a c a g a a t a t a t a a c a t c g t a g g t g t c t g g g t g a a c a g t t t a t t c c t g g c a c c g c c c a a t g t g t c t t a t a t a t t g t a g c a t c c a c a g a c c c a c t t g t c a a a t a a g g a c c g t

420 GAP promoter t c c a c t a a a t a t a a t g g a g c c c g c t t t t t a a g c t g g c a t c c a g a a a a a a a a a g a a t c c c a a g g t g a t t t a t a t t a c c t c g g g c g a a a a a t t c g a c c g t a g g t c t t t t t t t t t c t t a g g g t

480 GAP promoter g c a c c a a a a t a t t g t t t t c t t c a c c a a c c a t c a g t t c a t a g g t c c a t t c t c t t a g c g c a a c g t g g t t t t a t a a c a a a a g a a g t g g t t g g t a g t c a a g t a t c c a g g t a a g a g a a t c g c g t t

540 GAP promoter c t a c a g a g a a c a g g g g c a c a a a c a g g c a a a a a a c g g g c a c a a c c t c a a t g g a g t g a t g c a g a t g t c t c t t g t c c c c g t g t t t g t c c g t t t t t t g c c c g t g t t g g a g t t a c c t c a c t a c g t

MfeI 600 GAP promoter a c c t g c c t g g a g t a a a t g a t g a c a c a a g g c a a t t g a c c c a c g c a t g t a t c t a t c t c a t t t t g g a c g g a c c t c a t t t a c t a c t g t g t t c c g t t a a c t g g g t g c g t a c a t a g a t a g a g t a a a

660 GAP promoter t c t t a c a c c t t c t a t t a c c t t c t g c t c t c t c t g a t t t g g a a a a a g c t g a a a a a a a a g g t t a g a a t g t g g a a g a t a a t g g a a g a c g a g a g a g a c t a a a c c t t t t t c g a c t t t t t t t t c c a a

720 GAP promoter C G G T A G G T GPDpro-F g a a a c c a g t t c c c t g a a a t t a t t c c c c t a c t t g a c t a a t a a g t a t a t a a a g a c g g t a g g t c t t t g g t c a a g g g a c t t t a a t a a g g g g a t g a a c t g a t t a t t c a t a t a t t t c t g c c a t c c a

780 GAP promoter A T T G A T T G T A A T T C T G GPDpro-F a t t g a t t g t a a t t c t g t a a a t c t a t t t c t t a a a c t t c t t a a a t t c t a c t t t t a t a g t t a g t a a c t a a c a t t a a g a c a t t t a g a t a a a g a a t t t g a a g a a t t t a a g a t g a a a a t a t c a a t c

840 GAP promoter t c t t t t t t t t a g t t t t a a a a c a c c a a g a a c t t a g t t t c g a a t a a a c a c a c a t a a a c a a a c a g a a a a a a a a t c a a a a t t t t g t g g t t c t t g a a t c a a a g c t t a t t t g t g t g t a t t t g t t t g

PacISalI 900 GAP promoter MVSKGEE a a a g t c g a c g g a t c g g t g a c g g t g c t g g t t t a a t t a a c a t g g t a a g t a a a g g t g a a g a g a t t t c a g c t g c c t a g c c a c t g c c a c g a c c a a a t t a a t t g t a c c a t t c a t t t c c a c t t c t c t

960 TTMGVIKPDMKIKLKMEGNV c a a c t a t g g g g g t a a t c a a a c c a g a c a t g a a g a t c a a a c t g a a a a t g g a a g g t a a c g t t a g t t g a t a c c c c c a t t a g t t t g g t c t g t a c t t c t a g t t t g a c t t t t a c c t t c c a t t g c a a t

MluI 1020 NGHAFVIEGEGEGKPYDGTN a t g g c c a c g c g t t t g t t a t t g a a g g t g a a g g a g a g g g g a a a c c t t a c g a c g g a a c c a a c a t a c c g g t g c g c a a a c a a t a a c t t c c a c t t c c t c t c c c c t t t g g a a t g c t g c c t t g g t t g t

1080 TINLEVKEGAPLPFSYDILT c t a t c a a t t t g g a g g t t a a g g a a g g t g c a c c a t t a c c a t t c a g c t a t g a c a t c t t a a c a a g a t a g t t a a a c c t c c a a t t c c t t c c a c g t g g t a a t g g t a a g t c g a t a c t g t a g a a t t g t t

1140 TAFSYGNRAFTKYPDDIPNY c t g c a t t t t c a t a c g g c a a c a g a g c t t t t a c c a a g t a c c c t g a c g a t a t a c c a a a t t a c t g a c g t a a a a g t a t g c c g t t g t c t c g a a a a t g g t t c a t g g g a c t g c t a t a t g g t t t a a t g a

1200 FKQSFPEGYSWERTMTFEDK t c a a a c a a t c c t t t c c t g a a g g t t a t t c a t g g g a g a g a a c a a t g a c g t t t g a a g a t a a g g a g t t t g t t a g g a a a g g a c t t c c a a t a a g t a c c c t c t c t t g t t a c t g c a a a c t t c t a t t c c

1260 GIVKVKSDISMEEDSFIYEI g c a t t g t c a a g g t c a a a t c t g a c a t t t c t a t g g a a g a g g a t t c a t t c a t c t a c g a g a t t c c g t a a c a g t t c c a g t t t a g a c t g t a a a g a t a c c t t c t c c t a a g t a a g t a g a t g c t c t a a g

PflMI 1320 HLKGENFPPNGPVMQKETTG a c c t c a a g g g c g a a a a c t t c c c a c c a a a t g g t c c a g t g a t g c a a a a g g a a a c t a c t g g a t t g g a g t t c c c g c t t t t g a a g g g t g g t t t a c c a g g t c a c t a c g t t t t c c t t t g a t g a c c t a

1380 WDASTERMYVRDGVLKGDVK g g g a t g c t t c t a c a g a a a g g a t g t a c g t g a g a g a t g g a g t c t t a a a a g g t g a t g t t a a a a c c c t a c g a a g a t g t c t t t c c t a c a t g c a c t c t c t a c c t c a g a a t t t t c c a c t a c a a t t t t

1440 MKLLLEGGGHHRVDFKTIYR t g a a g t t g c t a c t t g a a g g t g g t g g c c a t c a t a g a g t t g a t t t c a a a a c a a t c t a c a g a g a c t t c a a c g a t g a a c t t c c a c c a c c g g t a g t a t c t c a a c t a a a g t t t t g t t a g a t g t c t c

1500 AKKAVKLPDYHFVDHRIEIL c c a a a a a g g c t g t a a a a c t t c c t g a t t a t c a t t t t g t g g a t c a t c g t a t a g a a a t t c t a a g g t t t t t c c g a c a t t t t g a a g g a c t a a t a g t a a a a c a c c t a g t a g c a t a t c t t t a a g a t t

1560 NHDKDYNKVTVYEIAVARNS a c c a c g a c a a a g a t t a c a a t a a g g t c a c t g t t t a t g a a a t a g c a g t t g c c a g a a a t t c t a t g g t g c t g t t t c t a a t g t t a t t c c a g t g a c a a a t a c t t t a t c g t c a a c g g t c t t t a a g a t

BssHIIAscIBsrGI 1620 ADH1 terminator TDGMDELYK* c a g a t g g a a t g g a t g a a t t g t a c a a a t a a g g c g c g c c a c t t c t a a a t a a g c g a a t t t c t t g t c t a c c t t a c c t a c t t a a c a t g t t t a t t c c g c g c g g t g a a g a t t t a t t c g c t t a a a g a a

1680 ADH1 terminator C A C A T A T G T T T A A A A T pHybLex-R a t g a t t t a t g a t t t t t a t t a t t a a a t a a g t t a t a a a a a a a a t a a g t g t a t a c a a a t t t t a t a c t a a a t a c t a a a a a t a a t a a t t t a t t c a a t a t t t t t t t t a t t c a c a t a t g t t t a a a a t

1740 ADH1 terminator T T C A C T G A G pHybLex-R a a g t g a c t c t t a g g t t t t a a a a c g a a a a t t c t t a t t c t t g a g t a a c t c t t t c c t g t a g g t t t c a c t g a g a a t c c a a a a t t t t g c t t t t a a g a a t a a g a a c t c a t t g a g a a a g g a c a t c c a

1800 ADH1 terminator c a g g t t g c t t t c t c a g g t a t a g t a t g a g g t c g c t c t t a t t g a c c a c a c c t c t a c c g g c a g g t c c a a c g a a a g a g t c c a t a t c a t a c t c c a g c g a g a a t a a c t g g t g t g g a g a t g g c c g t c

BstEIIBglIII-SceI 1860 a t c c g c t a g g g a t a a c a g g g t a a t a t a g a t c t g t t t a g c t t g c c t c g t c c c c g c c g g g t c t a g g c g a t c c c t a t t g t c c c a t t a t a t c t a g a c a a a t c g a a c g g a g c a g g g g c g g c c c a g

1920 CEN/ARS a c c g a t c g c t t g c c t g t a a c t t a c a c g c g c c t c g c a t c t t t t a a t g a t g g a a t a a t t t g g t g g c t a g c g a a c g g a c a t t g a a t g t g c g c g g a g c g t a g a a a a t t a c t a c c t t a t t a a a c c

1980 CEN/ARS g a a t t t a c t c t g t a t t t a t t t a t t t t t a t g t t t t g t a t t t g g a t t t t a g a a a g t a a a t a a c t t a a a t g a g a c a t a a a t a a a t a a a a a t a c a a a a c a t a a a c c t a a a a t c t t t c a t t t a t t

2040 CEN/ARS a g a a g g t a g a a g a g t t a c g g a a t g a a g a a a a a a a a a t a a a c a a a g g t t t a a a a a a t t t c a t c t t c c a t c t t c t c a a t g c c t t a c t t c t t t t t t t t t a t t t g t t t c c a a a t t t t t t a a a g t

2100 CEN/ARS a c a a a a a g c g t a c t t t a c a t a t a t a t t t a t t a g a c a a g a a a a g c a g a t t a a a t a g a t a t a t g t t t t t c g c a t g a a a t g t a t a t a t a a a t a a t c t g t t c t t t t c g t c t a a t t t a t c t a t a t

2160 CEN/ARS c a t t c g a t t a a c g a t a a g t a a a a t g t a a a a t c a c a g g a t t t t c g t g t g t g g t c t t c t a c a g t a a g c t a a t t g c t a t t c a t t t t a c a t t t t a g t g t c c t a a a a g c a c a c a c c a g a a g a t g t

2220 CEN/ARS c a g a c a a g a t g a a a c a a t t c g g c a t t a a t a c c t g a g a g c a g g a a g a g c a a g a t a a a a g g t g t c t g t t c t a c t t t g t t a a g c c g t a a t t a t g g a c t c t c g t c c t t c t c g t t c t a t t t t c c a

2280 CEN/ARS a g t a t t t g t t g g c g a t c c c c c t a g a g t c t t t t a c a t c t t c g g a a a a c a a a a a c t a t t t t t t c a t a a a c a a c c g c t a g g g g g a t c t c a g a a a a t g t a g a a g c c t t t t g t t t t t g a t a a a a a

SwaI 2340 CEN/ARS t c t t t a a t t t c t t t t t t t a c t t t c t a t t t t t a a t t t a t a t a t t t a t a t t a a a a a a t t t a a a g a a a t t a a a g a a a a a a a t g a a a g a t a a a a a t t a a a t a t a t a a a t a t a a t t t t t t a a a t t

PmlI 2400 CEN/ARS a t t a t a a t t a t t t t t a t a g c a c g t g a t g a a a a g g a c c c t a a g a a a c c a t t a t t a t c a t g a t a a t a t t a a t a a a a a t a t c g t g c a c t a c t t t t c c t g g g a t t c t t t g g t a a t a a t a g t a c t

2460 c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t t c g g t g a t g g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a a a g c c a c t a c

2520 a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c

2580 a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g c g a t t c g g t a a t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c g c t a a g c c a t t

2640 t c t c c g a a c a g a a g g a a g a a c g a a g g a a g g a g c a c a g a c t t a g a t t g g t a t a t a t a c g c a a g a g g c t t g t c t t c c t t c t t g c t t c c t t c c t c g t g t c t g a a t c t a a c c a t a t a t a t g c g t

BsgI 2700 t a t g t a g t g t t g a a g a a a c a t g a a a t t g c c c a g t a t t c t t a a c c c a a c t g c a c a g a a c a a a t a c a t c a c a a c t t c t t t g t a c t t t a a c g g g t c a t a a g a a t t g g g t t g a c g t g t c t t g t t

SbfIPstI 2760 1 5 10 MSKATYKERAA URA3 MSKATYKERAA a a a c c t g c a g g a a a c g a a g a t a a a t c a t g t c g a a a g c t a c a t a t a a g g a a c g t g c t g c t a t t t g g a c g t c c t t t g c t t c t a t t t a g t a c a g c t t t c g a t g t a t a t t c c t t g c a c g a c g a t

2820 15 20 25 30 THPSPVAAKLFNIMHEKQTN URA3 THPSPVAAKLFNIMHEKQTN *GLGTAALSNLIMCSFCVFK c t c a t c c t a g t c c t g t t g c t g c c a a g c t a t t t a a t a t c a t g c a c g a a a a g c a a a c a a a c t g a g t a g g a t c a g g a c a a c g a c g g t t c g a t a a a t t a t a g t a c g t g c t t t t c g t t t g t t t g a

XcmI 2880 35 40 45 50 LCASLDVRTTKELLELVEAL URA3 LCASLDVRTTKELLELVEAL HAENSTRVVLSNSSNTSANP t g t g t g c t t c a t t g g a t g t t c g t a c c a c c a a g g a a t t a c t g g a g t t a g t t g a a g c a t t a g a c a c a c g a a g t a a c c t a c a a g c a t g g t g g t t c c t t a a t g a c c t c a a t c a a c t t c g t a a t c

NcoI 2940 55 60 65 70 GPKICLLKTHVDILTDFSME URA3 GPKICLLKTHVDILTDFSME GLIQKSFVCTSIKVSKEMSP g t c c c a a a a t t t g t t t a c t a a a a a c a c a t g t g g a t a t c t t g a c t g a t t t t t c c a t g g a g g c a g g g t t t t a a a c a a a t g a t t t t t g t g t a c a c c t a t a g a a c t g a c t a a a a a g g t a c c t c c

3000 75 80 85 90 GTVKPLKALSAKYNFLLFED URA3 GTVKPLKALSAKYNFLLFED VTLGSFANDALYLKKSKSSL g c a c a g t t a a g c c g c t a a a g g c a t t a t c c g c c a a g t a c a a t t t t t t a c t c t t c g a a g a c a c g t g t c a a t t c g g c g a t t t c c g t a a t a g g c g g t t c a t g t t a a a a a a t g a g a a g c t t c t g t

3060 95 100 105 110 RKFADIGNTVKLQYSAGVYR URA3 RKFADIGNTVKLQYSAGVYR FNASMPLVTLNCYEAPTYLI g a a a a t t t g c t g a c a t t g g t a a t a c a g t c a a a t t g c a g t a c t c t g c g g g t g t a t a c a g a a c t t t t a a a c g a c t g t a a c c a t t a t g t c a g t t t a a c g t c a t g a g a c g c c c a c a t a t g t c t t

PspOMI*ApaI*BsmI 3120 115 120 125 130 IAEWADITNAHGVVGPGIVS URA3 IAEWADITNAHGVVGPGIVS ASHASMVFACPTTPGPITLP t a g c a g a a t g g g c a g a c a t t a c g a a t g c a c a c g g t g t g g t g g g c c c a g g t a t t g t t a g c g a t c g t c t t a c c c g t c t g t a a t g c t t a c g t g t g c c a c a c c a c c c g g g t c c a t a a c a a t c g c

StuI 3180 135 140 145 150 GLKQAAEEVTKEPRGLLMLA URA3 GLKQAAEEVTKEPRGLLMLA KFCAASSTVFSGLPRKINAS g t t t g a a g c a g g c g g c a g a a g a a g t a a c a a a g g a a c c t a g a g g c c t t t t g a t g t t a g c a g c a a a c t t c g t c c g c c g t c t t c t t c a t t g t t t c c t t g g a t c t c c g g a a a a c t a c a a t c g t c

3240 155 160 165 170 ELSCKGSLSTGEYTKGTVDI URA3 ELSCKGSLSTGEYTKGTVDI NDHLPERDVPSYVLPVTSM a a t t g t c a t g c a a g g g c t c c c t a t c t a c t g g a g a a t a t a c t a a g g g t a c t g t t g a c a t t g t t a a c a g t a c g t t c c c g a g g g a t a g a t g a c c t c t t a t a t g a t t c c c a t g a c a a c t g t a a c

3300 175 180 185 190 AKSDKDFVIGFIAQRDMGGR URA3 AKSDKDFVIGFIAQRDMGGR c g a a g a g c g a c a a a g a t t t t g t t a t c g g c t t t a t t g c t c a a a g a g a c a t g g g t g g a a g a g g c t t c t c g c t g t t t c t a a a a c a a t a g c c g a a a t a a c g a g t t t c t c t g t a c c c a c c t t c t c

3360 195 200 205 210 DEGYDWLIMTPGVGLDDKGD URA3 DEGYDWLIMTPGVGLDDKGD a t g a a g g t t a c g a t t g g t t g a t t a t g a c a c c c g g t g t g g g t t t a g a t g a c a a g g g a g a c g t a c t t c c a a t g c t a a c c a a c t a a t a c t g t g g g c c a c a c c c a a a t c t a c t g t t c c c t c t g c

3420 215 220 225 230 ALGQQYRTVDDVVSTGSDII URA3 ALGQQYRTVDDVVSTGSDII c a t t g g g t c a a c a g t a t a g a a c c g t g g a t g a t g t g g t c t c t a c a g g a t c t g a c a t t a t t a g t a a c c c a g t t g t c a t a t c t t g g c a c c t a c t a c a c c a g a g a t g t c c t a g a c t g t a a t a a t

Bpu10I 3480 235 240 245 250 IVGRGLFAKGRDAKVEGERY URA3 IVGRGLFAKGRDAKVEGERY t t g t t g g a a g a g g a c t a t t t g c a a a g g g a a g g g a t g c t a a g g t a g a g g g t g a a c g t t a c a a a c a a c c t t c t c c t g a t a a a c g t t t c c c t t c c c t a c g a t t c c a t c t c c c a c t t g c a a t g t

Eco53kISacIEcoRI 3540 255 260 265 RKAGWEAYLRRCGQQN* URA3 RKAGWEAYLRRCGQQN* g a a a a g c a g g c t g g g a a g c a t a t t t g a g a a g a t g c g g c c a g c a a a a c t a a g a g c t c g a a t c t t t t c g t c c g a c c c t t c g t a t a a a c t c t t c t a c g c c g g t c g t t t t g a t t c t c g a g c t t a

SacIISfiIEagINotISpeIBspDIClaI 3600 T7 promoter G G G A T T7 t c a t c g a t g a t a t c a g a t c c a c t a g t g g c c t a t g c g g c c g c g g a t c t g c c g g t c t c c c t a a g t a g c t a c t a t a g t c t a g g t g a t c a c c g g a t a c g c c g g c g c c t a g a c g g c c a g a g g g a t

HpaI 3660 T7 promoter A T C A C T C A G C A T A A T T7 t a g t g a g t c g t a t t a a t t t c g a t a a g c c a g g t t a a c c t g c a t t a a t g a a t c g g c c a a c g c a t c a c t c a g c a t a a t t a a a g c t a t t c g g t c c a a t t g g a c g t a a t t a c t t a g c c g g t t g c g

3720 G A G C G A G T G A C T G A G C G A L4440 g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c

3780 c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t

3840 t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g

3900 ori a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c

3960 ori T T T C T A T G pBR322ori-F c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g

4020 ori G T C C G C A A A G G G pBR322ori-F c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g

4080 ori g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a

4140 ori a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g

4200 ori g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t

4260 ori c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t

4320 ori g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t

4380 ori t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t

4440 ori t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c

4500 ori c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c

4560 t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g

4620 t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a

BanI 4680 285 280 275 *WHKILSAGIEAIQRN AmpR *WHKILSAGIEAIQRN t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a

AhdI 4740 270 265 260 255 REDMTAQSGTTYIVVIRSPK AmpR REDMTAQSGTTYIVVIRSPK c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t

4800 250 245 240 235 GDPGLAAIIGRSGREGAGSK AmpR GDPGLAAIIGRSGREGAGSK c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t

NmeAIII 4860 230 225 220 215 DAIFWGAPLASRLLPGAVKD AmpR DAIFWGAPLASRLLPGAVKD t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g

4920 210 205 200 195 AEMWDILQQRSALTLLEGTL AmpR AEMWDILQQRSALTLLEGTL g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a

FspI 4980 190 185 180 175 LKRLTTAMAVPMTTDREDNP AmpR LKRLTTAMAVPMTTDREDNP a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a

5040 170 165 160 155 IAENLEPEWRDLRTVHDGMN AmpR IAENLEPEWRDLRTVHDGMN a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c

5100 150 145 140 135 HLFATLEKPGGITTLLLNAA AmpR HLFATLEKPGGITTLLLNAA t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t

TsoI 5160 130 125 120 115 TNDSMTIAASCLERVTMGDT AmpR TNDSMTIAASCLERVTMGDT g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t

5220 110 105 100 95 LHKETVPSYEVLDNQSYHIR AmpR LHKETVPSYEVLDNQSYHIR a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c

5280 90 85 80 75 RGLQEQGADIRSLVAGCLLV AmpR A T A A T A C C G C G C C A C A T A G C Amp-R RGLQEQGADIRSLVAGCLLV c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a

5340 70 65 60 55 KFTSMMPFREEPRFSELIKG AmpR KFTSMMPFREEPRFSELIKG t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c

5400 50 45 40 35 SNLDLEIYGVRAGLQDEADK AmpR SNLDLEIYGVRAGLQDEADK c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a

5460 30 25 VKVLTEPH 20 15 signal sequence AFVPLCFAAFFP AmpR VKVLTEPHAFVPLCFAAFFP a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t

5520 10 5 1 signal sequence ILAVRFHQISM AmpR AmpR promoter ILAVRFHQISM a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g

5580 AmpR promoter a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t

ZraIAatII 5640 AmpR promoter c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a

5700 a t t a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t a a t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a

3ʹ 5ʹ 5704 t t c g a a g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.