Addgene DL034:cFos::HaloTag-iPAK4 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: DL034:cFos::HaloTag-iPAK4 Sequencing Result


Map View

AmpR promoter SspI (11,476) Amp-R (11,222 .. 11,241) ScaI (11,152) PvuI (11,042) pBR322ori-F (9920 .. 9939) PciI (9779) L4440 (9669 .. 9686) CAP binding site lac promoter M13/pUC Reverse (9451 .. 9473) lac operator M13 Reverse (9438 .. 9454) M13 rev BstZ17I (9400) EBV-rev (9359 .. 9378) SV40pA-R (9305 .. 9324) FseI (9040) MauBI (8800) EM7 promoter AvrII (8635) SV40pro-F (8563 .. 8582) pBABE 3' (8316 .. 8336) F1ori-F (8176 .. 8197) F1ori-R (7966 .. 7985) BGH-rev (7603 .. 7620) PmeI (7587) KpnI (7010) Acc65I (7006) pBluescriptKS (6876 .. 6892) KS primer WPRE-R (6337 .. 6357) SgrDI (1) pRS-marker (47 .. 66) MluI (231) SpeI (252) NdeI (487) SnaBI (593) CMV-F (772 .. 792) NotI (1525) KflI - PpuMI (2311) PacI (2598) BstXI (2700) AfeI (3175) BstEII (4397) BsrGI (5625) DL034:cFos::HaloTag-iPAK4 11,592 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 A T G T T A G A C G A G A C pRS-marker g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a g a c

120 T A C G G C pRS-marker a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a

180 g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g

MluI 240 CMV enhancer t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g

SpeI 300 CMV enhancer a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a

360 CMV enhancer a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c

420 CMV enhancer a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a

480 CMV enhancer t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c

NdeI 540 CMV enhancer t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g

SnaBI 600 CMV enhancer a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c

660 CMV enhancer CMV promoter t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a

720 CMV promoter t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g

780 CMV promoter C G C A A A T G G CMV-F a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c

840 CMV promoter 5' LTR (truncated) G C G G T A G G C G T G CMV-F g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a

900 5' LTR (truncated) c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a

960 5' LTR (truncated) a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g

1020 5' LTR (truncated) t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g

1080 HIV-1 Ψ g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g

1140 HIV-1 Ψ g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t

1200 HIV-1 Ψ t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c

1260 g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t

1320 a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g

1380 t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t

1440 c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g

1500 a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t

NotI 1560 M a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c

1620 RDNWRSELYKYKVVKIEPLG a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t

1680 RRE VAPTKAKRRVVQREKRAVGI g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t

1740 RRE GALFLGFLGAAGSTMGAASM g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c

1800 RRE TLTVQARQLLSGIVQQQNNL a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c

1860 RRE LRAIEAQQHLLQLTVWGIKQ c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c

1920 RRE LQARILAVERYLKDQQLLGI c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a

1980 WGCSGKLICTTAVPWNASWS t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a

2040 NKSLEQIWNHTTWMEWDREI a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a

2100 1 K gp41 peptide NNYTSLIHSLIEESQNQQEK a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c

2160 5 10 15 NEQELLELDKWASL (in frame with gp41 peptide) WNWFNI gp41 peptide NEQELLELDKWASLWNWFNI a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t

2220 (in frame with gp41 peptide) TNWLWYIKLFIMIVGGLVGL TNWLWYIKLFIMIVGGLVGL a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t

2280 (in frame with gp41 peptide) RIVFAVLSIVNRVRQGYSPL RIVFAVLSIVNRVRQGYSPL a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t

PpuMIKflI 2340 (in frame with gp41 peptide) SFQTHLPTPRGPDRPEGIEE SFQTHLPTPRGPDRPEGIEE t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t

2400 (in frame with gp41 peptide) EGGERDRDRSIRLVNGSALR EGGERDRDRSIRLVNGSALR g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a

2460 (in frame with gp41 peptide) APILQTNGSIHPQF* cPPT/CTS APILQTNGSIHPQF* g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a

2520 cPPT/CTS t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t

2580 cPPT/CTS a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c

PacI 2640 a g a t c c a g t t t g g t t a a t t a a a a g c t t t c c t t t a g g a a c a g a g g c t t c g a g c c t t t a a g g t c t a g g t c a a a c c a a t t a a t t t t c g a a a g g a a a t c c t t g t c t c c g a a g c t c g g a a a t t c c

BstXI 2700 c t g c g t a c t t g c t t c t c c t a a t a c c a g a g a c t c a a a a a a a a a a a a a a a a g t t c c a g a t t g g a c g c a t g a a c g a a g a g g a t t a t g g t c t c t g a g t t t t t t t t t t t t t t t t c a a g g t c t a a c

2760 c t g g a c a a t g a c c c g g g t c t c a t c c c t t g a c c c t g g g a a c c g g g t c c a c a t t g a a t c a g g g a c c t g t t a c t g g g c c c a g a g t a g g g a a c t g g g a c c c t t g g c c c a g g t g t a a c t t a g t c c

2820 t g c g a a t g t t c g c t c g c c t t c t c t g c c t t t c c c g c c t c c c c t c c c c c g g c c g c g g c c c c g a c g c t t a c a a g c g a g c g g a a g a g a c g g a a a g g g c g g a g g g g a g g g g g c c g g c g c c g g g g c

2880 g t t c c c c c c c t g c g c t g c a c c c t c a g a g t t g g c t g c a g c c g g c g a g c t g t t c c c g t c a a t c a a g g g g g g g a c g c g a c g t g g g a g t c t c a a c c g a c g t c g g c c g c t c g a c a a g g g c a g t t a

2940 c c c t c c c t c c t t t a c a c a g g a t g t c c a t a t t a g g a c a t c t g c g t c a g c a g g t t t c c a c g g g g g a g g g a g g a a a t g t g t c c t a c a g g t a t a a t c c t g t a g a c g c a g t c g t c c a a a g g t g c c

3000 c c g g t c c c t g t t g t t c t g g g g g g g g g a c c a t c t c c g a a a t c c t a c a c g c g g a a g g t c t a g g g c c a g g g a c a a c a a g a c c c c c c c c c t g g t a g a g g c t t t a g g a t g t g c g c c t t c c a g a t c

3060 g a g a c c c c c t a a g a t c c c a a a t g t g a a c a c t c a t a g g t g a a a g a t g t a t g c c a a g a c g g g c t c t g g g g g a t t c t a g g g t t t a c a c t t g t g a g t a t c c a c t t t c t a c a t a c g g t t c t g c c c

3120 g g t t g a a a g c c t g g g g c g t a g a g t t g a c g a c a g a g c g c c c g c a g a g g g c c t t g g g g c g c g c c a a c t t t c g g a c c c c g c a t c t c a a c t g c t g t c t c g c g g g c g t c t c c c g g a a c c c c g c g c

AfeI 3180 c t t c c c c c c c c t t c c a g t t c c g c c c a g t g a c g t a g g a a g t c c a t c c a t t c a c a g c g c t t c g a a g g g g g g g g a a g g t c a a g g c g g g t c a c t g c a t c c t t c a g g t a g g t a a g t g t c g c g a a g

3240 (in frame with WELQut site) *ELRSQLSCSL t a t a a a g g c g c c a g c t g a g g c g c c t a c t a c t c c a a c c g c g a c t g c a g c g a g c a a c t g a g a a t a t t t c c g c g g t c g a c t c c g c g g a t g a t g a g g t t g g c g c t g a c g t c g c t c g t t g a c t c t

3300 (in frame with WELQut site) LSSLAPPEAFSCHGREWGAR a g a c t g g a t a g a g c c g g c g g t t c c g c g a a c g a g c a g t g a c c g c g c t c c c a c c c a g c t c t g t c t g a c c t a t c t c g g c c g c c a a g g c g c t t g c t c g t c a c t g g c g c g a g g g t g g g t c g a g a c

3360 S 1 QLEW WELQut site c t c t g c a g c t c c c a c c a g t g t c t a c c c c t g g a c c c c t t g c c g g g c t t t c c c c a a a c t t c g g a g a c g t c g a g g g t g g t c a c a g a t g g g g a c c t g g g g a a c g g c c c g a a a g g g g t t t g a a g c

3420 a c c a t g a t g t t c t c g g g t t t c a a c g c c g a c t a c g a g g c g t c a t c c t c c c g c t g c a g t a g c t g g t a c t a c a a g a g c c c a a a g t t g c g g c t g a t g c t c c g c a g t a g g a g g g c g a c g t c a t c g

3480 g c c t c c c c g g c c g g g g a c a g c c t t t c c t a c t a c c a t t c c c c a g c c g a c t c c t t c t c c a g c c g g a g g g g c c g g c c c c t g t c g g a a a g g a t g a t g g t a a g g g g t c g g c t g a g g a a g a g g t c g

3540 a t g g g c t c t c c t g t c a a c a c a c a g g t g a g t t t g g c t t t g t g t a g c c g c c a g g t c c g c g c t t a c c c g a g a g g a c a g t t g t g t g t c c a c t c a a a c c g a a a c a c a t c g g c g g t c c a g g c g c g a

3600 g a g g g t c g c c g t g g a g g a g a c a c t g g g g t g t g a c t c g c a g g g g c g g g g g g g t c t t c c t t t c t c c c a g c g g c a c c t c c t c t g t g a c c c c a c a c t g a g c g t c c c c g c c c c c c c a g a a g g a a a

3660 t t c g c t c t g g a g g g a g a c t g g c g c g g t c a g a g c a g c c t t a g c c t g g g a a c c c a g g a c t t g a a g c g a g a c c t c c c t c t g a c c g c g c c a g t c t c g t c g g a a t c g g a c c c t t g g g t c c t g a a c

3720 t c t g a g c g c g t g c a c a c t t g t c a t a g t a a g a c t t a g t g a c c c c t t c c c g c g c g g c a g g t t a g a c t c g c g c a c g t g t g a a c a g t a t c a t t c t g a a t c a c t g g g g a a g g g c g c g c c g t c c a a

3780 t a t t c t g a g t g g c c t g c c t g c a t t c t t c t c t c g g c c g a c t t g t t t c t g a g a t c a g c c g g g a t a a g a c t c a c c g g a c g g a c g t a a g a a g a g a g c c g g c t g a a c a a a g a c t c t a g t c g g c c c

3840 g c c a a c a a g t c t c g a g c a a a g a g t c g c t a a c t a g a g t t t g g g a g g c g g c a a a c c g c g g c a c g g t t g t t c a g a g c t c g t t t c t c a g c g a t t g a t c t c a a a c c c t c c g c c g t t t g g c g c c g t

3900 a t c c c c c c t c c c g g g g c a g c c t g g a g c a g g g a g g a g g g a g g a g g g a g g a g g g t g c t g c g g t a g g g g g g a g g g c c c c g t c g g a c c t c g t c c c t c c t c c c t c c t c c c t c c t c c c a c g a c g c c

3960 g c g g g t g t g t a a g g c a g t t t c a t t g a t a a a a a g c g a g t t c a t t c t g g a g a c t c c g g a g c a c g c c c a c a c a t t c c g t c a a a g t a a c t a t t t t t c g c t c a a g t a a g a c c t c t g a g g c c t c g t

4020 g c g c c t g c g t c a g c g c a g a c g t c a g g g a t a t t t a t a a c a a a c c c c c t t t c g a g c g a g t g a c g c g g a c g c a g t c g c g t c t g c a g t c c c t a t a a a t a t t g t t t g g g g g a a a g c t c g c t c a c t

4080 t g c c g a a g g g a t a a c g g g a a c g c a g c a g t a g g a t g g a g g g g a a a g g c t g c g c t g c g g a a t a c g g c t t c c c t a t t g c c c t t g c g t c g t c a t c c t a c c t c c c c t t t c c g a c g c g a c g c c t t a

4140 t c a a g g g a g g a t a t t g g g a g a g c t t t t a t c t c c g a t g a g g t g c a t a c a g g a a g a c a t a a g a g t t c c c t c c t a t a a c c c t c t c g a a a a t a g a g g c t a c t c c a c g t a t g t c c t t c t g t a t t c

4200 *AVSPGSAG c a g t c t c t g a c c g g a a t g c t t c t c t c t c c c t g c t t c a t g c g a c a c t a g g g c c a c t t g c t c g t c a g a g a c t g g c c t t a c g a a g a g a g a g g g a c g a a g t a c g c t g t g a t c c c g g t g a a c g a g

4260 GTDPVEESVEAKRKKNQKLV c a c c t g t g t c t g g a a c c t c c t c g c t c a c c t c c g c t t t c c t c t t t t t g t t t t g t t t c a g g a g t g g a c a c a g a c c t t g g a g g a g c g a g t g g a g g c g a a a g g a g a a a a a c a a a a c a a a g t c c t

4320 MIGTGFPFDPHY KACIEGSGHDTSAKWEVGMI c t t t t g c g c a g a t c t c t c c g g a t c c a t g a t c g g t a c t g g c t t t c c a t t c g a c c c c c a t t a g a a a a c g c g t c t a g a g a g g c c t a g g t a c t a g c c a t g a c c g a a a g g t a a g c t g g g g g t a a t

4380 VEVLGERMHYVDVGPRDGTP HFDQALAHVVDINTRAIAGR t g t g g a a g t c c t g g g c g a g c g c a t g c a c t a c g t c g a t g t t g g t c c g c g c g a t g g c a c c c c a c a c c t t c a g g a c c c g c t c g c g t a c g t g a t g c a g c t a c a a c c a g g c g c g c t a c c g t g g g g

BstEII 4440 VLFLHGNPTSSYVWRNIIPH HQEQVTVRGGGVHPAVDDRM t g t g c t g t t c c t g c a c g g t a a c c c g a c c t c c t c c t a c g t g t g g c g c a a c a t c a t c c c g c a a c a c g a c a a g g a c g t g c c a t t g g g c t g g a g g a g g a t g c a c a c c g c g t t g t a g t a g g g c g t

4500 VAPTHRCIAPDLIGMGKSDK NCRGMAANSWVQDTHAFGVF t g t t g c a c c g a c c c a t c g c t g c a t t g c t c c a g a c c t g a t c g g t a t g g g c a a a t c c g a c a a a c a a c g t g g c t g g g t a g c g a c g t a a c g a g g t c t g g a c t a g c c a t a c c c g t t t a g g c t g t t

4560 PDLGYFFDDHVRFMDAFIEA WVQTIEEVVVDAEHIGEDFG a c c a g a c c t g g g t t a t t t c t t c g a c g a c c a c g t c c g c t t c a t g g a t g c c t t c a t c g a a g c t g g t c t g g a c c c a a t a a a g a a g c t g c t g g t g c a g g c g a a g t a c c t a c g g a a g t a g c t t c g

4620 LGLEEVVLVIHDWGSALGFH QTQFLDDQDNVVPAGSQTEV c c t g g g t c t g g a a g a g g t c g t c c t g g t c a t t c a c g a c t g g g g c t c c g c t c t g g g t t t c c a g g a c c c a g a c c t t c t c c a g c a g g a c c a g t a a g t g c t g a c c c c g a g g c g a g a c c c a a a g g t

4680 WAKRNPERVKGIAFMEFIRP PGLAIWLADFTNCKHLEDAR c t g g g c c a a g c g c a a t c c a g a g c g c g t c a a a g g t a t t g c a t t t a t g g a g t t c a t c c g c c c g a c c c g g t t c g c g t t a g g t c t c g c g c a g t t t c c a t a a c g t a a a t a c c t c a a g t a g g c g g g

4740 IPTWDEWPEFARETFQAFRT DRGPVFPWFKGALGELGEAG t a t c c c g a c c t g g g a c g a a t g g c c a g a a t t t g c c c g c g a g a c c t t c c a g g c c t t c c g c a c a t a g g g c t g g a c c c t g c t t a c c g g t c t t a a a c g g g c g c t c t g g a a g g t c c g g a a g g c g t g

4800 TDVGRKLIIDQNVFIEGTLP GVDAALQDDILVNKDLTRQR c a c c g a c g t c g g c c g c a a g c t g a t c a t c g a t c a g a a c g t t t t t a t c g a g g g t a c g c t g c c g t g g c t g c a g c c g g c g t t c g a c t a g t a g c t a g t c t t g c a a a a a t a g c t c c c a t g c g a c g g

4860 MGVVRPLTEVEMDHYREPFL HTDDARQSFDLHVM g a t g g g t g t c g t c c g c c c g c t g a c t g a a g t c g a g a t g g a c c a t t a c c g c g a g c c g t t c c t c t a c c c a c a g c a g g c g g g c g a c t g a c t t c a g c t c t a c c t g g t a a t g g c g c t c g g c a a g g a

4920 NPVDREPLWRFPNELPIAGE g a a t c c t g t t g a c c g c g a g c c a c t g t g g c g c t t c c c a a a c g a g c t g c c a a t c g c c g g t g a c t t a g g a c a a c t g g c g c t c g g t g a c a c c g c g a a g g g t t t g c t c g a c g g t t a g c g g c c a c t

4980 PANIVALVEEYMDWLHQSPV g c c a g c g a a c a t c g t c g c g c t g g t c g a a g a a t a c a t g g a c t g g c t g c a c c a g t c c c c t g t c g g t c g c t t g t a g c a g c g c g a c c a g c t t c t t a t g t a c c t g a c c g a c g t g g t c a g g g g a c a

5040 PKLLFWGTPGVLIPPAEAAR c c c g a a g c t g c t g t t c t g g g g c a c c c c a g g c g t t c t g a t c c c a c c g g c c g a a g c c g c t c g g g g c t t c g a c g a c a a g a c c c c g t g g g g t c c g c a a g a c t a g g g t g g c c g g c t t c g g c g a g c

5100 LAKSLPNCKAVDIGPGLNLL c c t g g c c a a a a g c c t g c c t a a c t g c a a g g c t g t g g a c a t c g g c c c g g g t c t g a a t c t g c t g g a c c g g t t t t c g g a c g g a t t g a c g t t c c g a c a c c t g t a g c c g g g c c c a g a c t t a g a c g a

5160 QEDNPDLIGSEIARWLSTLE g c a a g a a g a c a a c c c g g a c c t g a t c g g c a g c g a g a t c g c g c g c t g g c t g t c g a c g c t c g a c g t t c t t c t g t t g g g c c t g g a c t a g c c g t c g c t c t a g c g c g c g a c c g a c a g c t g c g a g c t

5220 ISGEPTTSGGSEAEDWTAAL g a t t t c c g g c g a g c c a a c c a c t t c a g g t g g a t c c g a a g c a g a g g a c t g g a c a g c a g c c c t c t a a a g g c c g c t c g g t t g g t g a a g t c c a c c t a g g c t t c g t c t c c t g a c c t g t c g t c g g g a

5280 LNRGRSRQPLVLGDNCFADL a c t g a a c a g g g g t c g c a g t c g c c a g c c c c t g g t a c t a g g g g a c a a t t g c t t t g c t g a c t t t g a c t t g t c c c c a g c g t c a g c g g t c g g g g a c c a t g a t c c c c t g t t a a c g a a a c g a c t g a a

5340 VHNWMELPEEFPAAPAVPGP g g t g c a c a a c t g g a t g g a g c t g c c t g a g g a a t t c c c c g c c g c c c c t g c t g t t c c t g g g c c c c a c g t g t t g a c c t a c c t c g a c g g a c t c c t t a a g g g g c g g c g g g g a c g a c a a g g a c c c g g

5400 PGPRSPQREPQRVSHEQFRA *WLPLWLSYGMLLEPS c c c t g g c c c c c g c t c a c c a c a g c g g g a g c c a c a g c g a g t a t c c c a t g a g c a g t t c c g g g c g g g a c c g g g g g c g a g t g g t g t c g c c c t c g g t g t c g c t c a t a g g g t a c t c g t c a a g g c c c g

5460 ALQLVVDPGDPRSYLDNFIK GQLQHHVWAVGAGVQVVEDL t g c c c t g c a g c t g g t g g t g g a c c c a g g c g a c c c c c g c t c c t a c c t g g a c a a c t t c a t c a a a c g g g a c g t c g a c c a c c a c c t g g g t c c g c t g g g g g c g a g g a t g g a c c t g t t g a a g t a g t t

5520 IGEGSTGIVCIATVRSSGKL NALAGRADHADGGHAARALQ g a t t g g c g a g g g c t c c a c g g g c a t c g t g t g c a t c g c c a c c g t g c g c a g c t c g g g c a a g c t c t a a c c g c t c c c g a g g t g c c c g t a g c a c a c g t a g c g g t g g c a c g c g t c g a g c c c g t t c g a

5580 VAVKKMDLRKQQRRELLFNE HGDLLHVQALLLPALQEEVL g g t g g c c g t c a a g a a g a t g g a c c t g c g c a a g c a g c a g a g g c g c g a g c t g c t c t t c a a c g a c c a c c g g c a g t t c t t c t a c c t g g a c g c g t t c g t c g t c t c c g c g c t c g a c g a g a a g t t g c t

BsrGI 5640 VVIMRDYQHENVVEMYNSYL HYDHPVVLVLIHHLHVVAVQ g g t g g t a a t c a t g a g g g a c t a c c a g c a c g a g a a t g t g g t g g a g a t g t a c a a c a g c t a c c t c c a c c a t t a g t a c t c c c t g a t g g t c g t g c t c t t a c a c c a c c t c t a c a t g t t g t c g a t g g a

5700 VGDELWVVMEFLEGGALTDI HPVLEPHDHLEQFSAGEGVD g g t g g g g g a c g a g c t c t g g g t g g t c a t g g a g t t c c t g g a a g g a g g c g c c c t c a c c g a c a t c c a c c c c c t g c t c g a g a c c c a c c a g t a c c t c a a g g a c c t t c c t c c g c g g g a g t g g c t g t a

5760 VTHTRMNEEQIAAVCLAVLQ DGVGPHVLLLDCGHAKCHQL c g t c a c c c a c a c c a g g a t g a a c g a g g a g c a g a t c g c a g c c g t g t g c c t t g c a g t g c t g c a g c a g t g g g t g t g g t c c t a c t t g c t c c t c g t c t a g c g t c g g c a c a c g g a a c g t c a c g a c g t

5820 ALSVLHAQGVIHRDIKSDSI GQRHEVGLADDVPVDLAVRD g g c c c t g t c g g t g c t c c a c g c c c a g g g c g t c a t c c a c c g g g a c a t c a a g a g c g a c t c g a t c c g g g a c a g c c a c g a g g t g c g g g t c c c g c a g t a g g t g g c c c t g t a g t t c t c g c t g a g c t a

5880 LLTHDGRVKLSDFGFCAQVS QQGM c c t g c t g a c c c a t g a t g g c a g g g t g a a g c t g t c a g a c t t t g g g t t c t g c g c c c a g g t g a g g g a c g a c t g g g t a c t a c c g t c c c a c t t c g a c a g t c t g a a a c c c a a g a c g c g g g t c c a c t c

5940 KEVPRRKSLVGTPYWMAPEL c a a g g a a g t g c c c c g a a g g a a g t c g c t g g t c g g c a c g c c c t a c t g g a t g g c c c c a g a g c t g t t c c t t c a c g g g g c t t c c t t c a g c g a c c a g c c g t g c g g g a t g a c c t a c c g g g g t c t c g a

6000 ISRLPYGPEVDIWSLGIMVI c a t c t c c c g c c t t c c c t a c g g g c c a g a g g t a g a c a t c t g g t c g c t g g g g a t a a t g g t g a t g t a g a g g g c g g a a g g g a t g c c c g g t c t c c a t c t g t a g a c c a g c g a c c c c t a t t a c c a c t a

6060 EMVDGEPPYFNEPPLKAMKM t g a g a t g g t g g a c g g a g a g c c c c c c t a c t t c a a c g a g c c a c c c c t c a a a g c c a t g a a g a t a c t c t a c c a c c t g c c t c t c g g g g g g a t g a a g t t g c t c g g t g g g g a g t t t c g g t a c t t c t a

6120 IRDNLPPRLKNLHKVSPSLK g a t t c g g g a c a a c c t g c c a c c c c g a c t g a a g a a c c t g c a c a a g g t g t c g c c a t c c c t g a a c t a a g c c c t g t t g g a c g g t g g g g c t g a c t t c t t g g a c g t g t t c c a c a g c g g t a g g g a c t t

6180 GFLDRLLVRDPAQRATAAEL g g g c t t c c t g g a c c g c c t g c t g g t g c g a g a c c c t g c c c a g c g g g c c a c g g c a g c c g a g c t c c c g a a g g a c c t g g c g g a c g a c c a c g c t c t g g g a c g g g t c g c c c g g t g c c g t c g g c t c g a

6240 LKHPFLAKAGPPASIVPLMR g c t g a a g c a c c c a t t c c t g g c c a a g g c a g g g c c g c c t g c c a g c a t c g t g c c c c t c a t g c g c g a c t t c g t g g g t a a g g a c c g g t t c c g t c c c g g c g g a c g g t c g t a g c a c g g g g a g t a c g c

6300 WPRE QNRTR* c c a g a a c c g c a c c a g a t g a g a a t t c g a t a t c a a g c t t a t c g a t a a t c a a c c t c t g g a t t a g g t c t t g g c g t g g t c t a c t c t t a a g c t a t a g t t c g a a t a g c t a t t a g t t g g a g a c c t a a t

6360 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c

6420 WPRE MPLYHAIASRMAFIFS MLLLPVWLSFS a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g

6480 WPRE SLYKSWLLSLYEELWPVVRQ PPCINPGCCLFMRSCGPLSG c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t

6540 WPRE RGVVCTVFADATPTGWGIAT NVAWCALCLLTQPPLVGALP a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g

6600 WPRE TCQLLSGTFAFPLPIATAEL PPVSSFPGLSLSPSLLPRRN c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a

6660 WPRE IAACLARCWTGARLLGTDNS SSPPALPAAGQGLGCWALTI c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g

6720 WPRE (in frame with Factor Xa site) *RGKRPQEGTNGGP VVLSGKSSSFPWLLACVATW PWCCRGNHRPFLGCSPVLPP c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g g c t g c t c g c c t g t g t t g c c a c c t g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a c c g a c g a g c g g a c a c a a c g g t g g a c

6780 WPRE (in frame with Factor Xa site) NQAPRGEAVDR 1 RGEI Factor Xa site ILRGTSFCYVPSALNPADLP GFCAGRPSATSLRPSIQRTF g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g

6840 WPRE SRGLLPALRPLPRLRLRPQT LPAACCRLCGLFRVFAFALR t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g

6900 WPRE KS primer C T A T G G C A G C T G G A G C T pBluescriptKS SRISLWAASPHRYRRPRDLE RVGSPFGPPPRIDTVDLET* g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a t c g a t a c c g t c g a c c t c g a g a c c t a g a c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t a g c t a t g g c a g c t g g a g c t c t g g a t c t

6960 KHGAITSSNTAATNADCAWL a a a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t t t t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a

Acc65IKpnI 7020 EAQEEEEVGFPVTPQVPLRP a g a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c t c t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g

7080 MTYKAAVDLSHFLKEKGGLE a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t

7140 GLIHSQRRQDILDLWIYHTQ a g g g c t a a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c a t c c c g a t t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t g t

7200 GYFPDWQNYTPGPGIRYPLT a g g c t a c t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t c c a c t g a c t c c g a t g a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a g g t g a c t g

7260 FGWCYKLVPVEQEKVEEANE c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g c c a a t g a g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t c g g t t a c t

7320 GENTRLLHPVSLHGMDDPER a g g a g a g a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g a g t c c t c t c t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c t c

7380 EVLEWRFDSRLAFHHMAREL a g a a g t a t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c c g a g a g c t t c t t c a t a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g g c t c t c g a

7440 5' LTR (truncated) HPDCTGSLWLDQI* g c a t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c g t a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c

7500 5' LTR (truncated) c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a

7560 5' LTR (truncated) g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a

PmeI 7620 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a c a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t

7680 bGH poly(A) signal g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t

7740 bGH poly(A) signal c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g

7800 bGH poly(A) signal a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t

7860 bGH poly(A) signal g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c

7920 f1 ori g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c

7980 f1 ori G A G G A A A G C G A A A G A F1ori-R t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a

8040 f1 ori A G G G A F1ori-R t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g

8100 f1 ori c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c

8160 f1 ori a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a

8220 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c

8280 f1 ori t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g

8340 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c

8400 SV40 promoter a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g

8460 SV40 promoter a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a

8520 SV40 promoter SV40 ori c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g

8580 SV40 promoter SV40 ori T A T T T A T G C A G A G G C C G A SV40pro-F c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t

AvrII 8640 SV40 promoter SV40 ori G G SV40pro-F g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c

8700 SV40 promoter EM7 promoter c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a

8760 EM7 promoter g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t

MauBI 8820 1 5 10 15 MAKLTSAVPVLTARDVAGA BleoR a c c a t g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g t g g t a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c

8880 20 25 30 35 VEFWTDRLGFSRDFVEDDFA BleoR g t c g a g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c c a g c t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g

8940 40 45 50 55 GVVRDDVTLFISAVQDQVVP BleoR g g t g t g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g c c a c a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c

9000 60 65 70 75 DNTLAWVWVRGLDELYAEWS BleoR g a c a a c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g c t g t t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c

FseI 9060 80 85 90 95 EVVSTNFRDASGPAMTEIGE BleoR g a g g t c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c t c c a g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c

9120 100 105 110 115 QPWGREFALRDPAGNCVHFV BleoR c a g c c g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g t c g g c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c

9180 120 125 AEEQD* BleoR g c c g a g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a c g g c t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t

9240 a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a

9300 SV40 poly(A) signal c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t

9360 SV40 poly(A) signal G T EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a

BstZ17I 9420 SV40 poly(A) signal G G T T T G T C C A A A C T C A T C EBV-rev g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c

9480 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a

9540 -10 -35 lac promoter CAP binding site c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g

9600 CAP binding site t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g

9660 c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a

9720 G A G C G A G T G A C T G A G C G A L4440 t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t

PciI 9780 g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g

9840 ori a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a

9900 ori t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c

9960 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g

10,020 ori t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g

10,080 ori g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g

10,140 ori a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g

10,200 ori t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a

10,260 ori a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a

10,320 ori a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g

10,380 ori t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a

10,440 ori t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c

10,500 a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g

10,560 a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t

10,620 285 *WHKILS AmpR *WHKILS t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c

10,680 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c

10,740 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t

10,800 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c

10,860 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t

10,920 200 195 190 185 ALTLLEGTLLKRLTTAMAVP AmpR ALTLLEGTLLKRLTTAMAVP g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g

10,980 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t

11,040 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c

PvuI 11,100 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a

ScaI 11,160 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g

11,220 100 95 90 85 LDNQSYHIRRGLQEQGADIR AmpR LDNQSYHIRRGLQEQGADIR a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c

11,280 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c

11,340 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a

11,400 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t

11,460 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR AmpR promoter PLCFAAFFPILAVRFHQISM g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t

SspI 11,520 AmpR promoter c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g

11,580 AmpR promoter a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t

3ʹ 5ʹ 11,592 g t g c c a c c t g a c c a c g g t g g a c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.