Addgene V17V Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: V17V Sequencing Result

Map View

600 400 200 End (695) BssSI - BssSαI (681) PstI (661) SfcI (657) BtsI - BtsαI (654) PfoI * (580) EXFP-R (541 .. 560) AcuI (471) BaeGI - Bsp1286I - Bme1580I (451) BsgI (318) Bpu10I (259) BlpI (241) EGFP-C (192 .. 213) BsrGI (152) BamHI (144) BsaWI (138) XbaI * (132) BclI * (122) BsaBI * (121) DraI (81) BsmI (34) MfeI (29) HpaI - HincII (20) Start (0) VC155 V17V 695 bp

Sequence View

BsmIMfeIHincIIHpaIStart (0) 5ʹ 3ʹ 60 a t t a a g c t g c a t a a a c a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g t a a t t c g a c g t a t t t g t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c

DraI 120 MAD g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a

BsrGIBamHIBsaWIXbaI*BsaBI*BclI* 180 (in frame with VC155) *RSGTSG 235 230 KYLEDMGLTI VC155 YDQLSRSGGSLVQLVHAESD *IRHIGQVARGHRSHD *RSGTSGKYLEDMGLTI a t g a t c a g t t a t c t a g a t c c g g t g g a t c c c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c t a c t a g t c a a t a g a t c t a g g c c a c c t a g g g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g

240 225 220 215 210 GAATVFELLVMHDRKENPDK VC155 G T G C T T G A G G T C G T C C T G G T A C EGFP-C PGGGHELQQDHVIALLVGVF RRRDRVGAPGHSRAERQPRQ GAATVFELLVMHDRKENPDK c c g g c g g c g g t c a c g a a c t c c a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g g g c c g c c g c c a g t g c t t g a g g t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c

Bpu10IBlpI 300 205 200 195 190 SLKSQYSLYHNDPLLVPGDG VC155 AQLGLVAQVVVVGQQHGAVA EAQVPLEPLPQRAAARPRRR SLKSQYSLYHNDPLLVPGDG c t c a g c t t g g a c t g g t a g c t c a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g g a g t c g a a c c t g a c c a t c g a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c

BsgI 360 185 180 175 170 IPTNQQYHDALQVGGDEINH VC155 DGGVLLVVVGELHAAVLDVV HPHEAPLPRRAARRRGRHQP IPTNQQYHDALQVGGDEINH a t g g g g g t g t t c t g c t g g t a g t g g t c g g c g a g c t g c a c g c c g c c g t c c t c g a t g t t g t g g t a c c c c c a c a a g a c g a c c a t c a c c a g c c g c t c g a c g t g c g g c g g c a g g a g c t a c a a c a c c

420 165 160 155 RIKFNAKIGNKQKD VC155 ADLEVGLDAVLLLVGGDIDV PDQLQGQHREEAQRRHYLRQ RIKFNAKIGNKQKDATIYVN c g g a t c t t g a a g t t g g c c t t g a t g c c g t t c t t c t g c t t g t c g g c g g t g a t a t a g a c g t t g g c c t a g a a c t t c a a c c g g a a c t a c g g c a a g a a g a c g a a c a g c c g c c a c t a t a t c t g c a a c

AcuIBme1580IBsp1286IBaeGI 480 VAVVVVLQLVPQDVAVLLEV PQQLQVGAQAGPHQRGGQLR HSNYNYELKHGLINGDEKFD t g g c t g t t g t a g t t g t a c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a c c g a c a a c a t c a a c a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c

540 DALQLDAVHQGVALELHLGA HGEARHPEGPHRGRVEGRRP IGKLEIRNVLTDGEFKVEAR a t g c c c t t c a g c t c g a t g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g t a c g g g a a g t c g a g c t a c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c

PfoI* 600 G T C T T G T A G T T G C C G T C G T C EXFP-R GLVVAVVLEEDGALLDVAFG DQLQRRGQLLHHAGPRLRRA TKYNGDDKFFITREQVYGEP g t c t t g t a g t t g c c g t c g t c c t t g a a g a a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c c a g a a c a t c a a c g g c a g c a g g a a c t t c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g

BtsαIBtsISfcI 660 HGGLEEVVLLHVVGVAGEAL HRVQLLRAAEHPRPLPRLVA MASKFFDHQKMHDPYRAFCQ a t g g c g g a c t t g a a g a a g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g g c g a a g c a c t g c t a c c g c c t g a a c t t c t t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c c g c t t c g t g a c g

End (695) BssSαIBssSIPstI 3ʹ 5ʹ 695 QAVAEGGHEGGP PRLRLHDRPHA LGYGFTTVLTPW a g g c c g t a g c c g a a g g t g g t c a c g a g g g t g g g c c a t c c g g c a t c g g c t t c c a c c a g t g c t c c c a c c c g g t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.