Addgene pLib6.6 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pLib6.6 Sequencing Result


Map View

pGEX 3' (7627 .. 7649) pRS-marker (7508 .. 7527) M13/pUC Forward (7292 .. 7314) M13 Forward (7283 .. 7300) M13 fwd attB PshAI (7213) HpaI (7040) SV40pA-R (6984 .. 7003) EBV-rev (6930 .. 6949) PaeR7I - PspXI - XhoI (6899) DraIII (6870) CsiI - SexAI * (6866) Puro-F (6795 .. 6815) BssHII (6754) EagI (6747) StuI (6630) SacII (6513) BstEII (6433) RsrII (6415) BsiWI (6355) PflFI - Tth111I (6341) Puro-R (6299 .. 6318) BmtI (6279) NheI (6275) EGFP-C (6154 .. 6175) EGFP-N (5546 .. 5567) PmeI (5496) BlpI (5340) AfeI (5338) NcoI (5162) PpuMI (5158) SpeI (4857) AflII (4833) BspDI - ClaI (4607) NsiI (4322) pBRforEco (10 .. 28) Amp-R (419 .. 438) ScaI (507) AhdI (988) pBR322ori-F (1721 .. 1740) L4440 (1974 .. 1991) BspQI - SapI (1993) lac promoter M13/pUC Reverse (2187 .. 2209) lac operator M13 Reverse (2206 .. 2222) M13 rev BfuAI - BspMI (2240) PstI - SbfI (2251) XbaI (2259) BstBI (2314) Acc65I (2902) KpnI (2906) BglII (3036) PflMI * (3291) AsiSI (3299) BbvCI (3411) NruI (3423) pLib6.6 7677 bp

Sequence View

5ʹ 3ʹ 60 C G G A G C A C T A T G C G G A T A A pBRforEco g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a

120 AmpR promoter c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a

180 AmpR promoter t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a

240 1 5 10 signal sequence MSIQHFRVALIPF AmpR promoter AmpR MSIQHFRVALIPF a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a

300 15 20 signal sequence FAAFCLPVFA 25 30 HPETLVKVKD AmpR FAAFCLPVFAHPETLVKVKD t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c

360 35 40 45 50 AEDQLGARVGYIELDLNSGK AmpR AEDQLGARVGYIELDLNSGK c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t

420 55 60 65 70 ILESFRPEERFPMMSTFKVL AmpR C G Amp-R ILESFRPEERFPMMSTFKVL t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g

480 75 80 85 90 LCGAVLSRIDAGQEQLGRRI AmpR A T A C A C C G C G C C A T A A T A Amp-R LCGAVLSRIDAGQEQLGRRI t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g

ScaI 540 95 100 105 110 HYSQNDLVEYSPVTEKHLTD AmpR HYSQNDLVEYSPVTEKHLTD a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c

600 115 120 125 130 GMTVRELCSAAITMSDNTAA AmpR GMTVRELCSAAITMSDNTAA g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t

660 135 140 145 150 NLLLTTIGGPKELTAFLHNM AmpR NLLLTTIGGPKELTAFLHNM a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c

720 155 160 165 170 GDHVTRLDRWEPELNEAIPN AmpR GDHVTRLDRWEPELNEAIPN g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c

780 175 180 185 190 DERDTTMPVAMATTLRKLLT AmpR DERDTTMPVAMATTLRKLLT a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c

840 195 200 205 210 GELLTLASRQQLIDWMEADK AmpR GELLTLASRQQLIDWMEADK g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c

900 215 220 225 230 VAGPLLRSALPAGWFIADKS AmpR VAGPLLRSALPAGWFIADKS t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c

960 235 240 245 250 GAGERGSRGIIAALGPDGKP AmpR GAGERGSRGIIAALGPDGKP g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a

AhdI 1020 255 260 265 270 SRIVVIYTTGSQATMDERNR AmpR SRIVVIYTTGSQATMDERNR c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g

1080 275 280 285 QIAEIGASLIKHW* AmpR QIAEIGASLIKHW* a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a

1140 c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t

1200 t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a

1260 ori c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a

1320 ori g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g

1380 ori t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g

1440 ori t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g

1500 ori t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c

1560 ori g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a

1620 ori c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c

1680 ori a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c

1740 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a

1800 ori a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c

1860 ori g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a

1920 g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t

1980 A G C G A G T L4440 t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g c c g a a c g a c c g a g c g c a g c g a g t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t c g g c t t g c t g g c t c g c g t c g c t c a

BspQISapI 2040 C A G T G A G C G A G L4440 c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a a a c c g c c t c t c c c c g c g c g t t g g c g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t t t g g c g g a g a g g g g c g c g c a a c c g

2100 c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g a c t g g a a a g c g g g c a g t g a g c g c a g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c t g a c c t t t c g c c c g t c a c t c g c g t

2160 -35 CAP binding site lac promoter a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g

2220 -10 lac promoter lac operator M13 rev A G C G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G M13 Reverse c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c

XbaISbfIPstIBspMIBfuAI 2280 M13 rev attB A C M13 Reverse a c c a t g a t t a c g c c a a g c t t g c a t g c c t g c a g g t c g a c t c t a g a g g a t c c c c c g g g t g c c t g g t a c t a a t g c g g t t c g a a c g t a c g g a c g t c c a g c t g a g a t c t c c t a g g g g g c c c a c g g

BstBI 2340 attB a g g g c g t g c c c t t g g g c t c c c c g g g c g c g t a c t t c g a a a a t t t t t g a a t a g c c c a g g t t g t c c c g c a c g g g a a c c c g a g g g g c c c g c g c a t g a a g c t t t t a a a a a c t t a t c g g g t c c a a c

2400 a t a a a a a t t c a t t t c a t a c g t t t t a t a a c t t a t g c c c c t a a g t a t t t t t t g a c c a t a g t g t a t t t t t a a g t a a a g t a t g c a a a a t a t t g a a t a c g g g g a t t c a t a a a a a a c t g g t a t c a c

2460 t t t c a a t t c t a c a t t a a t t t t a c a g a g t a g a a t g a a a c g c c a c c t a c t c a g c c a a g a g g c a a a g t t a a g a t g t a a t t a a a a t g t c t c a t c t t a c t t t g c g g t g g a t g a g t c g g t t c t c c g

2520 g a a a a g g t t a g c t c g c c a a g c a g a g a g g g c g c c a g t g c t c a c t a c t t t t t a t a a t t c t c a c t t t t c c a a t c g a g c g g t t c g t c t c t c c c g c g g t c a c g a g t g a t g a a a a a t a t t a a g a g t

2580 a c t t c t t t t t c c a g a c t c a g t t c g t a t a t a t a g a c c t a t t t t c a a t t t a a c g t c g g g g t c t g a a g a a a a a g g t c t g a g t c a a g c a t a t a t a t c t g g a t a a a a g t t a a a t t g c a g c c c c a g

2640 gRNA scaffold t t c g a g a a g a c c t g t t t t a g a g c t a g a a a t a g c a a g t t a a a a t a a g g c t a g t c c g t t a t c a a g c t c t t c t g g a c a a a a t c t c g a t c t t t a t c g t t c a a t t t t a t t c c g a t c a g g c a a t a g

2700 gRNA scaffold a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t t t t t g c c t a c c t g g a g c c t g a g a g t t g t t t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a a a a a c g g a t g g a c c t c g g a c t c t c a a c a a

2760 c a a t a a a a t a a a a a t g t t t c g t t t t t t t g c t t t c g c c a g t a t t t a t t a t t t t t c a t c a a t g t t a t t t t a t t t t t a c a a a g c a a a a a a a c g a a a g c g g t c a t a a a t a a t a a a a a g t a g t t a

2820 a t g t a t t c a a t t t g g t a t g t a t t t a g t a a t t g t a a t a t a t a g a c a a t g g t t t t c c g t t g a t a c a t a a g t t a a a c c a t a c a t a a a t c a t t a a c a t t a t a t a t c t g t t a c c a a a a g g c a a c t

2880 c g t a c a t a c a t c t g a c g t g t g t t t a t t t a g a c a t a a t a g t t a t g t t t t c a c a t c t t t t t a g c a t g t a t g t a g a c t g c a c a c a a a t a a a t c t g t a t t a t c a a t a c a a a a g t g t a g a a a a a t

Acc65IKpnI 2940 a t g t t c g c t t a a t g c g t a t g c g g t a c c c a g c a t g c a a t t c t a t a t t c t a a a a a c a c a a a t t a c a a g c g a a t t a c g c a t a c g c c a t g g g t c g t a c g t t a a g a t a t a a g a t t t t t g t g t t t a

3000 Ac5 promoter g a t a c t t c t a a a a a a a a a t c a t g a a t g g c a t c a a c t c t g a a t c a a a t c t t t g c a g a t g c a c t a t g a a g a t t t t t t t t t a g t a c t t a c c g t a g t t g a g a c t t a g t t t a g a a a c g t c t a c g t

BglII 3060 Ac5 promoter c c t a c t t c t c a t t t c c a c t g t c a c a t c a t t t t t c c a g a t c t c g c t g c c t g t t a t g t g g c c g g a t g a a g a g t a a a g g t g a c a g t g t a g t a a a a a g g t c t a g a g c g a c g g a c a a t a c a c c g g

3120 Ac5 promoter c a c a a a c c a a g a c a c g t t t t a t g g c c a t t a a a g c t g g c t g a t c g t c g c c a a a c a c c a a a t g t g t t t g g t t c t g t g c a a a a t a c c g g t a a t t t c g a c c g a c t a g c a g c g g t t t g t g g t t t a

3180 Ac5 promoter a c a t a a t g a a t a t g t a c a c a t t c g a g a a a g a a g c g a t c a a a g a a g c a t c t t c g g g c g g a g t g t a t t a c t t a t a c a t g t g t a a g c t c t t t c t t c g c t a g t t t c t t c g t a g a a g c c c g c c t c

3240 Ac5 promoter t a g g a g a a t g c g g a g g a g a a g g a g a a c g a g c t g a t c t a g t a t c t c t c c a c a a t c c a a t g c a t c c t c t t a c g c c t c c t c t t c c t c t t g c t c g a c t a g a t c a t a g a g a g g t g t t a g g t t a c g

AsiSIPflMI* 3300 Ac5 promoter c a a c t g a c c a a c t g g c c a t a t t c g g a g c a a t t t g a a g c c a a t t t c c a t c g c c t g g c g a t c g t t g a c t g g t t g a c c g g t a t a a g c c t c g t t a a a c t t c g g t t a a a g g t a g c g g a c c g c t a g

3360 Ac5 promoter g c t c c a t t c t t g g c t a t a t g t t t t t c a c c g t t a c c c g g g g c c a t t t t c a a a g a c t c g t c g c g a g g t a a g a a c c g a t a t a c a a a a a g t g g c a a t g g g c c c c g g t a a a a g t t t c t g a g c a g c

BbvCI 3420 Ac5 promoter g c a a g a t a a g a t t g t g t c a c t c g c t g t c t c t c t t c a t t t g t c g a a g a a t g c t g a g g a a t t c g t t c t a t t c t a a c a c a g t g a g c g a c a g a g a g a a g t a a a c a g c t t c t t a c g a c t c c t t a a

NruI 3480 Ac5 promoter t c g c g a t g a c g t c g g c g a g t a t t t t g a a g a a t g a g a a t a a t t t g t a t t t a t a c g a a a a t c a g c g c t a c t g c a g c c g c t c a t a a a a c t t c t t a c t c t t a t t a a a c a t a a a t a t g c t t t t a g

3540 Ac5 promoter a g t t a g t g g a a t t t t c t a c a a a a a c a t g t t a t c t a t a g a t a a t t t t g t t g c a a a a t a t g t t c a a t c a c c t t a a a a g a t g t t t t t g t a c a a t a g a t a t c t a t t a a a a c a a c g t t t t a t a c a

3600 Ac5 promoter t g a c t a t g a c a a a g a t t g t a t g t a t a t a c c t t t a a t g t a t t c t c a t t t t c t t a t g t a t t t a c t g a t a c t g t t t c t a a c a t a c a t a t a t g g a a a t t a c a t a a g a g t a a a a g a a t a c a t a a a

3660 Ac5 promoter a t a a t g g c a a t g a t g a t a c t g a t g a t a t t t t a a g a t g a t g c c a g a c c a a a a g g c t t g a a t t a t t a c c g t t a c t a c t a t g a c t a c t a t a a a a t t c t a c t a c g g t c t g g t t t t c c g a a c t t a

3720 Ac5 promoter t t c t g c g t c t t t t g c c g a a c g c a g t g c a t g t g c a a t t g t t g t t t t t t g g a a t a t t c a a t t a a g a c g c a g a a a a c g g c t t g c g t c a c g t a c a c g t t a a c a a c a a a a a a c c t t a t a a g t t a a

3780 Ac5 promoter t t c g g a c t g t c c g c t t t g a t t t c a g t t t c t t g g c t t a t t c a a a a a g c a a a g t a a a g c c a a a a g c c t g a c a g g c g a a a c t a a a g t c a a a g a a c c g a a t a a g t t t t t c g t t t c a t t t c g g t t

3840 Ac5 promoter MRQNGSGRKGVRG a a a a g c g a g a t g g c a a t a c c a a a t g c g g c a a a a c g g t a g t g g a a g g a a a g g g g t g c g g g g t t t t c g c t c t a c c g t t a t g g t t t a c g c c g t t t t g c c a t c a c c t t c c t t t c c c c a c g c c c c

3900 Ac5 promoter SGRKGGAGRGGVCGWARRHR c a g c g g a a g g a a g g g t g g g g c g g g g c g t g g c g g g g t c t g t g g c t g g g c g c g a c g t c a c c g g t c g c c t t c c t t c c c a c c c c g c c c c g c a c c g c c c c a g a c a c c g a c c c g c g c t g c a g t g g c

3960 Ac5 promoter RWSHSFDHVCVCVLFVSRHS a c g t t g g a g c c a c t c c t t t g a c c a t g t g t g c g t g t g t g t a t t a t t c g t g t c t c g c c a c t c t g c a a c c t c g g t g a g g a a a c t g g t a c a c a c g c a c a c a c a t a a t a a g c a c a g a g c g g t g a g

4020 Ac5 promoter PVVFFFLCCALSSAISLTHA g c c g g t t g t t t t t t t c t t t t t a t g c t g c g c t c t c t c t a g c g c c a t c t c g c t t a c g c a t g c c g g c c a a c a a a a a a a g a a a a a t a c g a c g c g a g a g a g a t c g c g g t a g a g c g a a t g c g t a c g

4080 Ac5 promoter QRTACCRFLLCVILARNRQL t c a a c g c a c c g c a t g t t g c c g t t t c c t t t t a t g c g t c a t t t t g g c t c g a a a t a g g c a a t t a g t t g c g t g g c g t a c a a c g g c a a a g g a a a a t a c g c a g t a a a a c c g a g c t t t a t c c g t t a a

4140 Ac5 promoter FKQRLVNENAKINKSTIWLL a t t t a a a c a a a g a t t a g t c a a c g a a a a c g c t a a a a t a a a t a a g t c t a c a a t a t g g t t a c t t a a a t t t g t t t c t a a t c a g t t g c t t t t g c g a t t t t a t t t a t t c a g a t g t t a t a c c a a t g a

4200 Ac5 promoter IAMCVQPTIATKATTQVAFP t a t t g c c a t g t g t g t g c a g c c a a c g a t a g c a a c a a a a g c a a c a a c a c a g g t g g c t t t c c c a t a a c g g t a c a c a c a c g t c g g t t g c t a t c g t t g t t t t c g t t g t t g t g t c c a c c g a a a g g g

4260 Ac5 promoter LSLFVCKPRASKTARPANAI t c t t t c a c t t t t t g t t t g c a a g c c g c g t g c g a g c a a g a c g g c a c g a c c g g c a a a c g c a a t a g a a a g t g a a a a a c a a a c g t t c g g c g c a c g c t c g t t c t g c c g t g c t g g c c g t t t g c g t t a

4320 Ac5 promoter TLTKSRRSFGEKHQGA* t a c g c t g a c a a a g a g c a g a c g a a g t t t t g g c g a a a a a c a t c a a g g c g c c t g a t a c g a a t g a t g c g a c t g t t t c t c g t c t g c t t c a a a a c c g c t t t t t g t a g t t c c g c g g a c t a t g c t t a c

NsiI 4380 Ac5 promoter c a t t t g c a a t a a c a a t t g c g a t a t t t a a t a t t g t t t a t g a a g c t g t t t g a c t t c a a a a c a g t a a a c g t t a t t g t t a a c g c t a t a a a t t a t a a c a a a t a c t t c g a c a a a c t g a a g t t t t g t

4440 Ac5 promoter c a c a a a a a a a a a a a t a a a a c a a a t t a t t t g a a a g a g a a t t a g g a a t c g g a c g c t t a t c g t g t g t t t t t t t t t t t a t t t t g t t t a a t a a a c t t t c t c t t a a t c c t t a g c c t g c g a a t a g c a

4500 Ac5 promoter t a g g g t a a c a a c a a g a a a t g c t t a c t g a g t c a c a g c c t c t g g a a a a c t g c c g c a a g c c a g a t c c c a t t g t t g t t c t t t a c g a a t g a c t c a g t g t c g g a g a c c t t t t g a c g g c g t t c g g t c

4560 Ac5 promoter a g a g a g a g a g a a a a a g a g g g a g a g c a g c t t a g a c c g c a t g t g c t t g t g t g t g a g g c g t c t t c t c t c t c t c t t t t t c t c c c t c t c g t c g a a t c t g g c g t a c a c g a a c a c a c a c t c c g c a g a

BspDIClaI 4620 Ac5 promoter c t c t c t t c g t c t c t g t t g c g c a a a c g c a t a g a c t g c a c t g a a a a a a t c g a t t a c c t a t t t g a g a g a a g c a g a g a c a a c g c g t t t g c g t a t c t g a c g t g a c t t t t t t a g c t a a t g g a t a a a

4680 Ac5 promoter t t t a t g a a t g a a t a t t t g c a c t a t t a c t a t t c a a a a c t a t t a a g a t a g c a a t c a c a t t c a a a a t a c t t a c t t a t a a a c g t g a t a a t g a t a a g t t t t g a t a a t t c t a t c g t t a g t g t a a g t

4740 Ac5 promoter a t a g c c a a a t a c t a t a c c a c c t g a g c g a t g c a a c g a a a t g a t c a a t t t g a g c a a a a a t g c t a t c g g t t t a t g a t a t g g t g g a c t c g c t a c g t t g c t t t a c t a g t t a a a c t c g t t t t t a c g

4800 Ac5 promoter t g c a t a t t t a g g a c g g c a t c a t t a t a g a a a t g c t t c t t g c t g t g t a c t t t t c t c t c g t c t a c g t a t a a a t c c t g c c g t a g t a a t a t c t t t a c g a a g a a c g a c a c a t g a a a a g a g a g c a g a

SpeIAflII 4860 Ac5 promoter *FRSLNPTNEVVL g g c a g c t g t t t c g c c g t t a t t g t t a a a a c c g g c t t a a g t t a g g t g t g t t t t c t a c g a c t a c c g t c g a c a a a g c g g c a a t a a c a a t t t t g g c c g a a t t c a a t c c a c a c a a a a g a t g c t g a t

4920 Ac5 promoter SHGVLLHTNCLIDRSYGIQL g t g a a t g c c c t a c t a g a a g a t g t g t g t t g c a c a a a a t g t c c c t g g a a t a a c c a a t t t g a a c a c t t a c g g g a t g a t c t t c t a c a c a c a a c g t g t t t t a c a g g g a c c t t a t t g g t t a a a c t t

4980 Ac5 promoter ASLLLRLSIHINNLQLTKID g t g c a g a t a g c a g t a a a c g t a a g c t a a t a t g a a t a t t a t t t a a c t g t a a t g t t t t a a t a t c a c g t c t a t c g t c a t t t g c a t t c g a t t a t a c t t a t a a t a a a t t g a c a t t a c a a a a t t a t a

5040 Ac5 promoter SSMVLLGVIFVHVYTHKPMC c g c t g g a c a t t a c t a a t a a a c c c a c t a t a a a c a c a t g t a c a t a t g t a t g t t t t g g c a t a c g c g a c c t g t a a t g a t t a t t t g g g t g a t a t t t g t g t a c a t g t a t a c a t a c a a a a c c g t a t g

5100 Ac5 promoter HTTPSFIHLLVTVMVAYLYG a a t g a g t a g t t g g g g a a a a a a t g t g t a a a a g c a c c g t g a c c a t c a c a g c a t a a a g a t a a c t t a c t c a t c a a c c c c t t t t t t a c a c a t t t t c g t g g c a c t g g t a g t g t c g t a t t t c t a t t g

PpuMI 5160 Ac5 promoter ASTDFILLGWISDCAAVSLV c a g c t g a a g t a t c g a a t a t g a g t a a c c c c c a a a t t g a a t c a c a t g c c g c a a c t g a t a g g a g t c g a c t t c a t a g c t t a t a c t c a t t g g g g g t t t a a c t t a g t g t a c g g c g t t g a c t a t c c t

NcoI 5220 Ac5 promoter WPLVSKMAIYLVCVLVFVWN c c c a t g g a a g t a c a c t c t t c a t g g c g a t a t a c a a g a c a c a c a c a a g c a c g a a c a c c c a g t g g g t a c c t t c a t g t g a g a a g t a c c g c t a t a t g t t c t g t g t g t g t t c g t g c t t g t g g g t c a

5280 Ac5 promoter RLFNETFSFGIPSCNMGMYP t g c g g a g g a a a t t c t c c g t a a a t g a a a a c c c a a t c g g c g a a c a a t t c a t a c c c a t a t a t g a c g c c t c c t t t a a g a g g c a t t t a c t t t t g g g t t a g c c g c t t g t t a a g t a t g g g t a t a t a c

AfeI 5340 Ac5 promoter LLKSRSKLASLM g t a a a a g t t t t g a a c g c g a c t t g a g a g c g g a g a g c a t t g c g g c t g a t a a g g t t t t a g c g c c a t t t t c a a a a c t t g c g c t g a a c t c t c g c c t c t c g t a a c g c c g a c t a t t c c a a a a t c g c g

BlpI 5400 Ac5 promoter t a a g c g g g c t t t a t a a a a c g g g c t g c g g g a c c a g t t t t c a t a t c a c t a c c g t t t g a g t t c a t t c g c c c g a a a t a t t t t g c c c g a c g c c c t g g t c a a a a g t a t a g t g a t g g c a a a c t c a a g

5460 Ac5 promoter t t g t g c t g t g t g g a t a c t c c t c c c g a c a c a a a g c c g c t c c a t c a g c c a g c a g t c g t c t a a a a c a c g a c a c a c c t a t g a g g a g g g c t g t g t t t c g g c g a g g t a g t c g g t c g t c a g c a g a t t

PmeI 5520 1 M 1a V 5 SKGEE Ac5 promoter EGFP MVSKGEE t c c a g a g a c c c c g g a t c c a g g a a t t c g c c c t t g t t t a a a c a t g g t g a g c a a g g g c g a g g a a g g t c t c t g g g g c c t a g g t c c t t a a g c g g g a a c a a a t t t g t a c c a c t c g t t c c c g c t c c t

5580 10 15 20 25 LFTGVVPILVELDGDVNGHK EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LFTGVVPILVELDGDVNGHK g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t

5640 30 35 40 45 FSVSGEGEGDATYGKLTLKF EGFP FSVSGEGEGDATYGKLTLKF g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a

5700 50 55 60 65 ICTTGKLPVPWPTLVTTLTY EGFP ICTTGKLPVPWPTLVTTLTY c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t

5760 70 75 80 85 GVQCFSRYPDHMKQHDFFKS EGFP GVQCFSRYPDHMKQHDFFKS c g g c g t g c a g t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c g c c g c a c g t c a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g

5820 90 95 100 105 AMPEGYVQERTIFFKGDGNY EGFP AMPEGYVQERTIFFKGDGNY c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g g c g a c g g c a a c t a g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c c g c t g c c g t t g a t

5880 110 115 120 125 KTRAEVKFEGDTLVNRIELK EGFP KTRAEVKFEGDTLVNRIELK c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t

5940 130 135 140 145 GIDFKEDGNILGHKLEYNYN EGFP GIDFKEDGNILGHKLEYNYN g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t

6000 150 155 160 165 SHNVYIMADKQKNGIKVNFK EGFP SHNVYIMADKQKNGIKVNFK c a g c c a c a a c g t c t a t a t c a t g g c c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g t c g g t g t t g c a g a t a t a g t a c c g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t

6060 170 175 180 185 IRHNIEDGSVQLADHYQQNT EGFP IRHNIEDGSVQLADHYQQNT g a t c c g c c a c a a c a t c g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c t a g g c g g t g t t g t a g c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g

6120 190 195 200 205 PIGDGPVLLPDNHYLSTQSA EGFP PIGDGPVLLPDNHYLSTQSA c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g

6180 210 215 220 225 LSKDPNEKRDHMVLLEFVTA EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C LSKDPNEKRDHMVLLEFVTA c c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c g g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g

6240 230 235 AGITLGMDELYK L 140 145 EGRGSLL EGFP T2A AGITLGMDELYKLEGRGSLL * c g c c g g g a t c a c t c t c g g c a t g g a c g a g c t g t a c a a g c t t g a g g g c a g a g g a a g c c t t c t g c g g c c c t a g t g a g a g c c g t a c c t g c t c g a c a t g t t c g a a c t c c c g t c t c c t t c g g a a g a

BmtINheI 6300 150 155 TCGDVEENPGP (in frame with T2A) ASGSGSGS 1 M T2A PuroR T A Puro-R TCGDVEENPGPASGSGSGSM CATVHLLIGARSATAAATAH a a c a t g c g g t g a c g t g g a g g a g a a t c c c g g c c c t g c t a g c g g t a g c g g c a g c g g t a g c a t t t g t a c g c c a c t g c a c c t c c t c t t a g g g c c g g g a c g a t c g c c a t c g c c g t c g c c a t c g t a

BsiWITth111IPflFI 6360 5 10 15 20 TEYKPTVRLATRDDVPRAVR PuroR C T G G C T C A T G T T C G G G T G Puro-R TEYKPTVRLATRDDVPRAVR GLVLGRHAEGGAVVDGPGYA g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c c g g g c c g t a c g c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g g c c c g g c a t g c

RsrII 6420 25 30 35 40 TLAAAFADYPATRHTVDPDR PuroR TLAAAFADYPATRHTVDPDR GEGGREGVVGGRAVGDVRVA c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a c c c g g a c c g g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t g g g c c t g g c

BstEII 6480 45 50 55 60 HIERVTELQELFLTRVGLDI PuroR HIERVTELQELFLTRVGLDI VDLPDGLQLFEEERADPEVD c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a

SacII 6540 65 70 75 80 GKVWVADDGAAVAVWTTPES PuroR GKVWVADDGAAVAVWTTPES ALHPDRVVAGRHRDPGRRLA c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c

6600 85 90 95 100 VEAGAVFAEIGPRMAELSGS PuroR VEAGAVFAEIGPRMAELSGS DFRPRHEGLDARAHGLQATG c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g

StuI 6660 105 110 115 120 RLAAQQQMEGLLAPHRPKEP PuroR RLAAQQQMEGLLAPHRPKEP PQGRLLLHFAEQRRVPGLLG c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g

6720 125 130 135 140 AWFLATVGVSPDHQGKGLGS PuroR AWFLATVGVSPDHQGKGLGS RPEQGGDADRGVVLALTQAA c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c

BssHIIEagI 6780 145 150 155 160 AVVLPGVEAAERAGVPAFLE PuroR AVVLPGVEAAERAGVPAFLE GDHEGSHLRGLAGPHGGEQL c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t

6840 165 170 175 180 TSAPRNLPFYERLGFTVTAD PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F TSAPRNLPFYERLGFTVTAD GGRGAVEGEVLPEAEGDGGV g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t

PaeR7IPspXIXhoISexAI*CsiIDraIII 6900 185 190 195 200 VEVPEGPRTWCMTRKPGA* PuroR VEVPEGPRTWCMTRKPGA* DLHGFSRAGPAHGALGTGSE c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c t g a c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g a c t g a

6960 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev LSGSMILYMSSNPCVVVLIC c g a g g g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c g c t c c c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g

7020 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R HFFHKIQSIQSAIAKNTVMI a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t

HpaI 7080 SV40 poly(A) signal LQLLCTLLLLQM t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c

7140 g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a t g g c t g a t t c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t a c c g a c t a a

7200 a t g a t c a g t c g a c c g a t g c c c t t g a g a g c c t t c a a c c c a g t c a g c t c c t t c c g g t g g g c g t a c t a g t c a g c t g g c t a c g g g a a c t c t c g g a a g t t g g g t c a g t c g a g g a a g g c c a c c c g c

PshAI 7260 attB c g g g g c a t g a c t a t c g t c g c c g c a c t t a t g g t a c g c g c c c g g g g a g c c c a a g g g c a c g c c g c c c c g t a c t g a t a g c a g c g g c g t g a a t a c c a t g c g c g g g c c c c t c g g g t t c c c g t g c g g

7320 attB M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward c t g g c a c c c g a a g g g c g a a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c g a c c g t g g g c t t c c c g c t t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g

7380 c t g g c g t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g a c c g c a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t

7440 g c g a a g a g g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c c g c t t c t c c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g

7500 g c c t g a t g c g g t a t t t t c t c c t t a c g c a t c t g t g c g g t a t t t c a c a c c g c a t a t g g t g c a c g g a c t a c g c c a t a a a a g a g g a a t g c g t a g a c a c g c c a t a a a g t g t g g c g t a t a c c a c g t

7560 A T G T T A G A C G A G A C T A C G G C pRS-marker c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g c c c c g a c a c c c g c c a a c a c g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c g g g g c t g t g g g c g g t t g t g

7620 c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c c g g c a t c c g c t t a c a g a c a a g c t g t g a g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g g c c g t a g g c g a a t g t c t g t t c g a c a c t

3ʹ 5ʹ 7677 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t c a c c g t c a t c a c c g a a a c g c g c g a g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a g t g g c a g t a g t g g c t t t g c g c g c t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.