Addgene IBAR-Cry2PHR Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: IBAR-Cry2PHR Sequencing Result


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NheI (6917) CMV-F (6845 .. 6865) SnaBI (6666) NdeI (6560) AseI (6333) PciI (6268) pBR322ori-F (6113 .. 6132) EcoO109I (5448) TK-pA-R (5204 .. 5223) BstBI (5032) Neo-F (4886 .. 4905) RsrII (4866) PflFI - Tth111I (4468) FspI (4452) Neo-R (4276 .. 4295) BspDI * - ClaI * (4190) SfiI (4125) CsiI - SexAI * (3939) pBABE 3' (3853 .. 3873) F1ori-R (3591 .. 3610) BmtI (6921) BssHII (29) PmlI (205) AfeI (294) EcoRV (483) BspEI (563) AbsI - PaeR7I - PspXI - XhoI (751) EcoRI (1485) AccI (2177) TspMI - XmaI (2258) SmaI (2260) mCherry-R (2426 .. 2444) AhdI (2475) SbfI (2636) mCherry-F (2694 .. 2713) PflMI (2720) SgrAI (2962) BsrGI (2981) NotI (2994) XbaI * (3004) MfeI (3100) SV40pA-R (3151 .. 3170) BtsI - BtsαI (3189) EBV-rev (3205 .. 3224) AflII (3232) F1ori-F (3379 .. 3400) IBAR-Cry2PHR 6922 bp

Sequence View

BssHII 5ʹ 3ʹ 60 MEAVIEKECSALGGLFQTII a t g g a g g c t g t g a t t g a g a a g g a a t g c a g c g c g c t c g g a g g c c t c t t c c a g a c c a t c a t c t a c c t c c g a c a c t a a c t c t t c c t t a c g t c g c g c g a g c c t c c g g a g a a g g t c t g g t a g t a g

120 SDMKGSYPVWEDFINKAGKL a g c g a c a t g a a g g g g a g c t a t c c a g t t t g g g a a g a t t t c a t a a a c a a a g c a g g a a a g c t g t c g c t g t a c t t c c c c t c g a t a g g t c a a a c c c t t c t a a a g t a t t t g t t t c g t c c t t t c g a c

180 QSQLRTTVVAAAAFLDAFQK c a g t c c c a g c t t c g g a c a a c a g t a g t a g c a g c a g c t g c c t t c t t g g a c g c c t t t c a g a a a g t c a g g g t c g a a g c c t g t t g t c a t c a t c g t c g t c g a c g g a a g a a c c t g c g g a a a g t c t t t

PmlI 240 VADMATNTRGGTREIGSALT g t g g c t g a c a t g g c c a c c a a c a c a c g t g g t g g g a c c a g g g a g a t t g g a t c t g c t c t c a c c c a c c g a c t g t a c c g g t g g t t g t g t g c a c c a c c c t g g t c c c t c t a a c c t a g a c g a g a g t g g

AfeI 300 RMCMRHRSIEAKLRQFSSAL a g g a t g t g c a t g a g g c a c a g a a g c a t t g a a g c c a a g c t g a g g c a g t t t t c g a g c g c t t t a t c c t a c a c g t a c t c c g t g t c t t c g t a a c t t c g g t t c g a c t c c g t c a a a a g c t c g c g a a a t

360 IDCLINPLQEQMEEWKKVAN a t t g a t t g t c t g a t a a a c c c a c t t c a a g a a c a g a t g g a a g a a t g g a a g a a a g t g g c c a a c t a a c t a a c a g a c t a t t t g g g t g a a g t t c t t g t c t a c c t t c t t a c c t t c t t t c a c c g g t t g

420 QLDKDHAKEYKKARQEIKKK c a g c t g g a t a a a g a c c a c g c a a a a g a a t a t a a g a a a g c c c g c c a a g a g a t a a a a a a g a a g g t c g a c c t a t t t c t g g t g c g t t t t c t t a t a t t c t t t c g g g c g g t t c t c t a t t t t t t c t t c

480 SSDTLKLQKKAKKVDTLGRG t c c t c g g a t a c g c t g a a a c t g c a g a a g a a a g c a a a a a a a g t g g a c a c t c t t g g g a g a g g t a g g a g c c t a t g c g a c t t t g a c g t c t t c t t t c g t t t t t t t c a c c t g t g a g a a c c c t c t c c a

EcoRV 540 DIQPQLDSALQDVNDKYLLL g a t a t c c a g c c t c a g t t g g a c a g t g c t c t c c a a g a t g t c a a t g a t a a g t a t c t c t t a t t g c t a t a g g t c g g a g t c a a c c t g t c a c g a g a g g t t c t a c a g t t a c t a t t c a t a g a g a a t a a c

BspEI 600 EETEKQAVRKALIEERGRFC g a a g a a a c a g a a a a g c a g g c t g t c c g g a a g g c t t t g a t t g a a g a a c g t g g c c g a t t c t g t c t t c t t t g t c t t t t c g t c c g a c a g g c c t t c c g a a a c t a a c t t c t t g c a c c g g c t a a g a c a

660 TFISMLRPVIEEEISMLGEI a c c t t c a t c t c t a t g c t g c g g c c a g t g a t t g a a g a a g a a a t c t c a a t g c t a g g g g a a a t a t g g a a g t a g a g a t a c g a c g c c g g t c a c t a a c t t c t t c t t t a g a g t t a c g a t c c c c t t t a t

720 THLQTISEDLKSLTMDPHKL a c c c a c c t t c a g a c c a t c t c g g a a g a t c t a a a a a g c c t g a c c a t g g a c c c t c a c a a a c t g t g g g t g g a a g t c t g g t a g a g c c t t c t a g a t t t t t c g g a c t g g t a c c t g g g a g t g t t t g a c

PaeR7IPspXIXhoIAbsI 780 1 5 MKMDKK CRY2PHR PSSSEQVILDLEATMKMDKK c c c t c c t c a a g t g a a c a g g t g a t t c t g g a c c t c g a g g c c a c c a t g a a g a t g g a c a a a a a g g g g a g g a g t t c a c t t g t c c a c t a a g a c c t g g a g c t c c g g t g g t a c t t c t a c c t g t t t t t c

840 10 15 20 25 TIVWFRRDLRIEDNPALAAA CRY2PHR TIVWFRRDLRIEDNPALAAA a c t a t a g t t t g g t t t a g a a g a g a c c t a a g g a t t g a g g a t a a t c c t g c a t t a g c a g c a g c t t g a t a t c a a a c c a a a t c t t c t c t g g a t t c c t a a c t c c t a t t a g g a c g t a a t c g t c g t c g a

900 30 35 40 45 AHEGSVFPVFIWCPEEEGQF CRY2PHR AHEGSVFPVFIWCPEEEGQF g c t c a c g a a g g a t c t g t t t t t c c t g t c t t c a t t t g g t g t c c t g a a g a a g a a g g a c a g t t t c g a g t g c t t c c t a g a c a a a a a g g a c a g a a g t a a a c c a c a g g a c t t c t t c t t c c t g t c a a a

960 50 55 60 65 YPGRASRWWMKQSLAHLSQS CRY2PHR YPGRASRWWMKQSLAHLSQS t a t c c t g g a a g a g c t t c a a g a t g g t g g a t g a a a c a a t c a c t t g c t c a c t t a t c t c a a t c c a t a g g a c c t t c t c g a a g t t c t a c c a c c t a c t t t g t t a g t g a a c g a g t g a a t a g a g t t a g g

1020 70 75 80 85 LKALGSDLTLIKTHNTISAI CRY2PHR LKALGSDLTLIKTHNTISAI t t g a a g g c t c t t g g a t c t g a c c t c a c t t t a a t c a a a a c c c a c a a c a c g a t t t c a g c g a t c a a c t t c c g a g a a c c t a g a c t g g a g t g a a a t t a g t t t t g g g t g t t g t g c t a a a g t c g c t a g

1080 90 95 100 105 LDCIRVTGATKVVFNHLYDP CRY2PHR LDCIRVTGATKVVFNHLYDP t t g g a t t g t a t c c g c g t t a c c g g t g c t a c a a a a g t c g t c t t t a a c c a c c t c t a t g a t c c t a a c c t a a c a t a g g c g c a a t g g c c a c g a t g t t t t c a g c a g a a a t t g g t g g a g a t a c t a g g a

1140 110 115 120 125 VSLVRDHTVKEKLVERGISV CRY2PHR VSLVRDHTVKEKLVERGISV g t t t c g t t a g t t c g g g a c c a t a c c g t a a a g g a g a a g c t g g t g g a a c g t g g g a t c t c t g t g c a a a g c a a t c a a g c c c t g g t a t g g c a t t t c c t c t t c g a c c a c c t t g c a c c c t a g a g a c a c

1200 130 135 140 145 QSYNGDLLYEPWEIYCEKGK CRY2PHR QSYNGDLLYEPWEIYCEKGK c a a a g c t a c a a t g g a g a t c t a t t g t a t g a a c c g t g g g a g a t a t a c t g c g a a a a g g g c a a a g t t t c g a t g t t a c c t c t a g a t a a c a t a c t t g g c a c c c t c t a t a t g a c g c t t t t c c c g t t t

1260 150 155 160 165 PFTSFNSYWKKCLDMSIESV CRY2PHR PFTSFNSYWKKCLDMSIESV c c t t t t a c g a g t t t c a a t t c t t a c t g g a a g a a a t g c t t a g a t a t g t c g a t t g a a t c c g t t g g a a a a t g c t c a a a g t t a a g a a t g a c c t t c t t t a c g a a t c t a t a c a g c t a a c t t a g g c a a

1320 170 175 180 185 MLPPPWRLMPITAAAEAIWA CRY2PHR MLPPPWRLMPITAAAEAIWA a t g c t t c c t c c t c c t t g g c g g t t g a t g c c a a t a a c t g c a g c g g c t g a a g c g a t t t g g g c g t a c g a a g g a g g a g g a a c c g c c a a c t a c g g t t a t t g a c g t c g c c g a c t t c g c t a a a c c c g c

1380 190 195 200 205 CSIEELGLENEAEKPSNALL CRY2PHR CSIEELGLENEAEKPSNALL t g t t c g a t t g a a g a a c t a g g g c t g g a g a a t g a g g c c g a g a a a c c g a g c a a t g c g t t g t t a a c a a g c t a a c t t c t t g a t c c c g a c c t c t t a c t c c g g c t c t t t g g c t c g t t a c g c a a c a a t

1440 210 215 220 225 TRAWSPGWSNADKLLNEFIE CRY2PHR TRAWSPGWSNADKLLNEFIE a c t a g a g c t t g g t c t c c a g g a t g g a g c a a t g c t g a t a a g t t a c t a a a t g a g t t c a t c g a g t g a t c t c g a a c c a g a g g t c c t a c c t c g t t a c g a c t a t t c a a t g a t t t a c t c a a g t a g c t c

EcoRI 1500 230 235 240 245 KQLIDYAKNSKKVVGNSTSL CRY2PHR KQLIDYAKNSKKVVGNSTSL a a g c a g t t g a t a g a t t a t g c a a a g a a c a g c a a g a a a g t t g t t g g g a a t t c t a c t t c a c t a t t c g t c a a c t a t c t a a t a c g t t t c t t g t c g t t c t t t c a a c a a c c c t t a a g a t g a a g t g a t

1560 250 255 260 265 LSPYLHFGEISVRHVFQCAR CRY2PHR LSPYLHFGEISVRHVFQCAR c t t t c t c c g t a t c t c c a t t t c g g g g a a a t a a g c g t c a g a c a c g t t t t c c a g t g t g c c c g g g a a a g a g g c a t a g a g g t a a a g c c c c t t t a t t c g c a g t c t g t g c a a a a g g t c a c a c g g g c c

1620 270 275 280 285 MKQIIWARDKNSEGEESADL CRY2PHR MKQIIWARDKNSEGEESADL a t g a a a c a a a t t a t a t g g g c a a g a g a t a a g a a c a g t g a a g g a g a a g a a a g t g c a g a t c t t t a c t t t g t t t a a t a t a c c c g t t c t c t a t t c t t g t c a c t t c c t c t t c t t t c a c g t c t a g a a

1680 290 295 300 305 FLRGIGLREYSRYICFNFPF CRY2PHR FLRGIGLREYSRYICFNFPF t t t c t t a g g g g a a t c g g t t t a a g a g a g t a t t c t c g g t a t a t a t g t t t c a a c t t c c c g t t t a a a g a a t c c c c t t a g c c a a a t t c t c t c a t a a g a g c c a t a t a t a c a a a g t t g a a g g g c a a a

1740 310 315 320 325 THEQSLLSHLRFFPWDADVD CRY2PHR THEQSLLSHLRFFPWDADVD a c t c a c g a g c a a t c g t t g t t g a g t c a t c t t c g g t t t t t c c c t t g g g a t g c t g a t g t t g a t t g a g t g c t c g t t a g c a a c a a c t c a g t a g a a g c c a a a a a g g g a a c c c t a c g a c t a c a a c t a

1800 330 335 340 345 KFKAWRQGRTGYPLVDAGMR CRY2PHR KFKAWRQGRTGYPLVDAGMR a a g t t c a a g g c c t g g a g a c a a g g c a g g a c c g g t t a t c c g t t g g t g g a t g c c g g a a t g a g a t t c a a g t t c c g g a c c t c t g t t c c g t c c t g g c c a a t a g g c a a c c a c c t a c g g c c t t a c t c t

1860 350 355 360 365 ELWATGWMHNRIRVIVSSFA CRY2PHR ELWATGWMHNRIRVIVSSFA g a g c t t t g g g c t a c c g g a t g g a t g c a t a a c a g a a t a a g a g t g a t t g t t t c a a g c t t t g c t c t c g a a a c c c g a t g g c c t a c c t a c g t a t t g t c t t a t t c t c a c t a a c a a a g t t c g a a a c g a

1920 370 375 380 385 VKFLLLPWKWGMKYFWDTLL CRY2PHR VKFLLLPWKWGMKYFWDTLL g t g a a g t t t c t t c t c c t t c c a t g g a a a t g g g g a a t g a a g t a t t t c t g g g a t a c a c t t t t g c a c t t c a a a g a a g a g g a a g g t a c c t t t a c c c c t t a c t t c a t a a a g a c c c t a t g t g a a a a c

1980 390 395 400 405 DADLECDILGWQYISGSIPD CRY2PHR DADLECDILGWQYISGSIPD g a t g c t g a t t t g g a a t g t g a c a t c c t t g g c t g g c a g t a t a t c t c t g g g a g t a t c c c c g a t c t a c g a c t a a a c c t t a c a c t g t a g g a a c c g a c c g t c a t a t a g a g a c c c t c a t a g g g g c t a

2040 410 415 420 425 GHELDRLDNPALQGAKYDPE CRY2PHR GHELDRLDNPALQGAKYDPE g g c c a c g a g c t t g a t c g c t t g g a c a a t c c c g c g t t a c a a g g c g c c a a a t a t g a c c c a g a a c c g g t g c t c g a a c t a g c g a a c c t g t t a g g g c g c a a t g t t c c g c g g t t t a t a c t g g g t c t t

2100 430 435 440 445 GEYIRQWLPELARLPTEWIH CRY2PHR GEYIRQWLPELARLPTEWIH g g t g a g t a c a t a a g g c a a t g g c t t c c c g a g c t t g c g a g a t t g c c a a c t g a a t g g a t c c a t c c a c t c a t g t a t t c c g t t a c c g a a g g g c t c g a a c g c t c t a a c g g t t g a c t t a c c t a g g t a

2160 450 455 460 465 HPWDAPLTVLKASGVELGTN CRY2PHR HPWDAPLTVLKASGVELGTN c a t c c a t g g g a c g c t c c t t t a a c c g t a c t c a a a g c t t c t g g t g t g g a a c t c g g a a c a a a c g t a g g t a c c c t g c g a g g a a a t t g g c a t g a g t t t c g a a g a c c a c a c c t t g a g c c t t g t t t g

AccI 2220 470 475 480 485 YAKPIVDIDTARELLAKAIS CRY2PHR YAKPIVDIDTARELLAKAIS t a t g c g a a a c c c a t t g t a g a c a t c g a c a c a g c t c g t g a g c t a c t a g c t a a a g c t a t t t c a a t a c g c t t t g g g t a a c a t c t g t a g c t g t g t c g a g c a c t c g a t g a t c g a t t t c g a t a a a g t

TspMIXmaISmaI 2280 490 495 RTREAQIMIGAA (in frame with CRY2PHR) ARDPPVAT CRY2PHR Kozak sequence RTREAQIMIGAAARDPPVAT a g a a c c c g t g a a g c a c a g a t c a t g a t c g g a g c a g c a g c c c g g g a t c c a c c g g t c g c c a c c t c t t g g g c a c t t c g t g t c t a g t a c t a g c c t c g t c g t c g g g c c c t a g g t g g c c a g c g g t g g

2340 1 5 10 15 20 MVSKGEEDNMAIIKEFMRFK mCherry Kozak sequence MVSKGEEDNMAIIKEFMRFK a t g g t g a g c a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a a g t a c c a c t c g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t t c

2400 25 30 35 40 VHMEGSVNGHEFEIEGEGEG mCherry VHMEGSVNGHEFEIEGEGEG g t g c a c a t g g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c a c g t g t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g

2460 45 50 55 60 RPYEGTQTAKLKVTKGGPLP mCherry G G T T C G A C T T C C A C T G G T T mCherry-R RPYEGTQTAKLKVTKGGPLP c g c c c c t a c g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c g c g g g g a t g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g

AhdI 2520 65 70 75 80 FAWDILSPQFMYGSKAYVKH mCherry FAWDILSPQFMYGSKAYVKH t t c g c c t g g g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g c a c a a g c g g a c c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t c g t g

2580 85 90 95 100 PADIPDYLKLSFPEGFKWER mCherry PADIPDYLKLSFPEGFKWER c c c g c c g a c a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g g g c g g c t g t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g

SbfI 2640 105 110 115 120 VMNFEDGGVVTVTQDSSLQD mCherry VMNFEDGGVVTVTQDSSLQD g t g a t g a a c t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g a c c a c t a c t t g a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c t g

2700 125 130 135 140 GEFIYKVKLRGTNFPSDGPV mCherry C C C C G T A mCherry-F GEFIYKVKLRGTNFPSDGPV g g c g a g t t c a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c g t a c c g c t c a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g g c a t

PflMI 2760 145 150 155 160 MQKKTMGWEASSERMYPEDG mCherry A T G C A G A A G A A G A mCherry-F MQKKTMGWEASSERMYPEDG a t g c a g a a g a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a c g g c t a c g t c t t c t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t g c c g

2820 165 170 175 180 ALKGEIKQRLKLKDGGHYDA mCherry ALKGEIKQRLKLKDGGHYDA g c c c t g a a g g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a c g c t c g g g a c t t c c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t g c g a

2880 185 190 195 200 EVKTTYKAKKPVQLPGAYNV mCherry EVKTTYKAKKPVQLPGAYNV g a g g t c a a g a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a c g t c c t c c a g t t c t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t g c a g

2940 205 210 215 220 NIKLDITSHNEDYTIVEQYE mCherry NIKLDITSHNEDYTIVEQYE a a c a t c a a g t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a a t t g t a g t t c a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t t

NotIBsrGISgrAI 3000 225 230 235 RAEGRHSTGGMDELYK* mCherry RAEGRHSTGGMDELYK* c g c g c c g a g g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t a a a g c g g c c g c g c g c g g c t c c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a t t t c g c c g g c g

XbaI* 3060 g a c t c t a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c c t g a g a t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g

MfeI 3120 SV40 poly(A) signal t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a

3180 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c

AflIIBtsαIBtsI 3240 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c

3300 f1 ori t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t

3360 f1 ori t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c

3420 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g

3480 f1 ori a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g

3540 f1 ori t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g

3600 f1 ori A G G G A A G A A A F1ori-R c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t

3660 f1 ori G C G A A A G G A G F1ori-R g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g

3720 f1 ori a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a

3780 AmpR promoter g t g c g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g c a c g c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c

3840 AmpR promoter SV40 promoter SV40 enhancer a g a c a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a t c t g t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t

3900 SV40 promoter SV40 enhancer C C T T A C A C A C A G T C A A T C C C A pBABE 3' a g a a c c a g c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g t c t t g g t c g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c

SexAI*CsiI 3960 SV40 promoter SV40 enhancer g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c

4020 SV40 promoter SV40 enhancer g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g

4080 SV40 promoter SV40 enhancer SV40 ori c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g

SfiI 4140 SV40 promoter SV40 ori a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a

BspDI*ClaI* 4200 SV40 promoter SV40 ori t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c

4260 1 5 10 MIEQDGLHAGSPA NeoR/KanR 1 5 10 MIEQDGLHAGSPA NeoR/KanR MIEQDGLHAGSPA a c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c t g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g

4320 15 20 25 30 AWVERLFGYDWAQQTIGCSD NeoR/KanR 15 20 25 30 AWVERLFGYDWAQQTIGCSD NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AWVERLFGYDWAQQTIGCSD g c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t c g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a

4380 35 40 45 50 AAVFRLSAQGRPVLFVKTDL NeoR/KanR 35 40 45 50 AAVFRLSAQGRPVLFVKTDL NeoR/KanR AAVFRLSAQGRPVLFVKTDL g c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g c g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c

4440 55 60 65 70 SGALNELQDEAARLSWLATT NeoR/KanR 55 60 65 70 SGALNELQDEAARLSWLATT NeoR/KanR SGALNELQDEAARLSWLATT t c c g g t g c c c t g a a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g a g g c c a c g g g a c t t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c

Tth111IPflFIFspI 4500 75 80 85 90 GVPCAAVLDVVTEAGRDWLL NeoR/KanR 75 80 85 90 GVPCAAVLDVVTEAGRDWLL NeoR/KanR GVPCAAVLDVVTEAGRDWLL g g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a c c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t

4560 95 100 105 110 LGEVPGQDLLSSHLAPAEKV NeoR/KanR 95 100 105 110 LGEVPGQDLLSSHLAPAEKV NeoR/KanR LGEVPGQDLLSSHLAPAEKV *RVKSRGLFY t t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a a a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t

4620 115 120 125 130 SIMADAMRRLHTLDPATCPF NeoR/KanR 115 120 125 130 SIMADAMRRLHTLDPATCPF NeoR/KanR SIMADAMRRLHTLDPATCPF GDHSICHPPQMRKIRSGAWE t c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c a g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g

4680 135 140 145 150 DHQAKHRIERARTRMEAGLV NeoR/KanR 135 140 145 150 DHQAKHRIERARTRMEAGLV NeoR/KanR DHQAKHRIERARTRMEAGLV VVLRFMADLSCTSPHFGTKD g a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c c t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g

4740 155 160 165 170 DQDDLDEEHQGLAPAELFAR NeoR/KanR 155 160 165 170 DQDDLDEEHQGLAPAELFAR NeoR/KanR DQDDLDEEHQGLAPAELFAR ILIIQVFLMLPERWGFQEGP g a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c

4800 175 180 185 190 LKASMPDGEDLVVTHGDACL NeoR/KanR 175 180 185 190 LKASMPDGEDLVVTHGDACL NeoR/KanR LKASMPDGEDLVVTHGDACL ELRAHGVALIEDHGMAIGAQ c t c a a g g c g a g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g g a g t t c c g c t c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c

4860 195 200 205 210 PNIMVENGRFSGFIDCGRLG NeoR/KanR 195 200 205 210 PNIMVENGRFSGFIDCGRLG NeoR/KanR PNIMVENGRFSGFIDCGRLG RIDHHFIAAKRSEDVTAPQT c c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a

RsrII 4920 215 220 225 230 VADRYQDIALATRDIAEELG NeoR/KanR 215 220 225 230 VADRYQDIALATRDIAEELG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F VADRYQDIALATRDIAEELG HRVAILVYRQSGTINSFLKA g t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c c a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g

4980 235 240 245 250 GEWADRFLVLYGIAAPDSQR NeoR/KanR 235 240 245 250 GEWADRFLVLYGIAAPDSQR NeoR/KanR GEWADRFLVLYGIAAPDSQR AFPSVAEEHKVTDGSGIRLA g g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c c c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g

BstBI 5040 255 260 265 IAFYRLLDEFF* NeoR/KanR 255 260 265 IAFYRLLDEFF* NeoR/KanR IAFYRLLDEFF* M DGEIAKKVLEESRSEPTRFS a t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a t a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t

5100 TDQATPNLPSRDFDSTAAFY RGLSAWGAM c c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g g g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c

5160 ERLGFGIVFRDAGWMILQRG a a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g t t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c

5220 A C T T T G T G C C T T C C T C T TK-pA-R DLMLEFFAHPRGRLTETRKE a t c t c a t g c t g g a g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a t a g a g t a c g a c c t c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t

5280 HSV TK poly(A) signal G T T TK-pA-R TIPEGTRAMTAIKRQNKTHG c a a t a c c g g a a g g a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g g t t a t g g c c t t c c t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c

5340 HSV TK poly(A) signal VGSFVHKRGVRSQGWHSVDT t t g g g t c g t t t g t t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c a a c c c a g c a a a c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g

5400 PPRPHWGQYARVSSFSPPHP c a c c g a g a c c c c a t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c g t g g c t c t g g g g t a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g

EcoO109I 5460 PSSGEGPGLAANVGAAGPAI c a a g t t c g g g t g a a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g g t t c a a g c c c a c t t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c

5520 ASGYSYIL* c c t c a g g t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a g g a g t c c a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t

5580 t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a

5640 ori t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g

5700 ori t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g

5760 ori c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g

5820 ori c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g

5880 ori c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a

5940 ori c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a

6000 ori g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a

6060 ori a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a

6120 ori G G G A A A C G pBR322ori-F a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c

6180 ori C C T G G T A T C T T T pBR322ori-F c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a

6240 ori g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a

PciI 6300 t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c

AseI 6360 t g g a t a a c c g t a t t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a a c c t a t t g g c a t a a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t

6420 CMV enhancer g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g

6480 CMV enhancer t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c

6540 CMV enhancer c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c

NdeI 6600 CMV enhancer g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t

6660 CMV enhancer t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g

SnaBI 6720 CMV enhancer CMV promoter a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c

6780 CMV promoter c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c

6840 CMV promoter a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t

6900 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t

BmtINheI 3ʹ 5ʹ 6922 g t g a a c c g t c a g a t c c g c t a g c c a c t t g g c a g t c t a g g c g a t c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.