Addgene pDF0159 pCMV - huDisCas7-11 mammalian expression Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pDF0159 pCMV - huDisCas7-11 mammalian expression Sequencing Result


Map View

AmpR promoter Amp-R (7854 .. 7873) FspI (7526) BpmI (7374) BsaI (7365) pBR322ori-F (6552 .. 6571) DrdI (6519) L4440 (6301 .. 6318) BspQI - SapI (6295) CAP binding site M13/pUC Reverse (6083 .. 6105) lac operator M13 Reverse (6070 .. 6086) SphI (5975) BbsI (5964) BGH-rev (5769 .. 5786) PmeI (5753) 6xHis AgeI (5723) Bsu36I (5569) PfoI (5518) MreI - SgrAI (5262) PflFI - Tth111I (4776) XbaI (4692) StuI (4302) SgrDI (20) pRS-marker (70 .. 89) NruI (234) MluI (254) CMV-F (795 .. 815) NotI - SacII (879) T7 (886 .. 905) HindIII (1027) RsrII (1102) EcoRV (1865) BmgBI (2344) BssHII (2379) Acc65I (2515) KpnI (2519) MscI (3144) pDF0159 pCMV - huDisCas7-11 mammalian expression 8205 bp

Sequence View

SgrDI 5ʹ 3ʹ 60 c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c g a t c t c c c g a t c c c c t a g g g t c g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g c t a g a g g g c t a g g g g a t c c c a g

120 A T G T T A G A C G A G A C T A C G G C pRS-marker t a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g a t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c

180 t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a

NruI 240 g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a

MluI 300 CMV enhancer a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g

360 CMV enhancer g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c

420 CMV enhancer c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g

480 CMV enhancer c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g

540 CMV enhancer t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t

600 CMV enhancer t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g

660 CMV enhancer CMV promoter t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t

720 CMV promoter c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t

780 CMV promoter c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t

840 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c

SacIINotI 900 CMV promoter T7 promoter T A A T A C G A C T C A C T A T7 a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g a g a t c c g c g g c c g c t a a t a c g a c t c a c t a t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c t c t a g g c g c c g g c g a t t a t g c t g a g t g a t

960 T7 promoter T A G G G T7 MTTTMKISIEFLEP *NFEELR t a g g g a g a g c c g c c a c c a t g a c g a c t a c t a t g a a g a t t t c a a t t g a a t t c c t c g a g c c g t a t c c c t c t c g g c g g t g g t a c t g c t g a t g a t a c t t c t a a a g t t a a c t t a a g g a g c t c g g c a

1020 FRMTKWQESTRRNKNNKEFV KPHGFPLFASPSVLVVLFKN t t c g g a t g a c c a a a t g g c a g g a a a g c a c t a g g c g a a a c a a g a a c a a c a a g g a a t t t g t t c a a g c c t a c t g g t t t a c c g t c c t t t c g t g a t c c g c t t t g t t c t t g t t g t t c c t t a a a c a a g

HindIII 1080 RGQAFARWHRNKKDNTKGRP AALSKCSPMSILLIVCFPPR g c g g c c a a g c t t t t g c a a g a t g g c a t a g a a a t a a g a a g g a t a a c a c a a a a g g g c g g c c t t c g c c g g t t c g a a a a c g t t c t a c c g t a t c t t t a t t c t t c c t a t t g t g t t t t c c c g c c g g a a

RsrII 1140 YITGTLLRSAVIRSAENLLT VYSPCQQPGCYNSARFVQQC a c a t a a c t g g g a c a t t g c t g c g g t c c g c a g t a a t t a g a a g c g c g g a a a a c c t g c t g a c a t t g t a t t g a c c c t g t a a c g a c g c c a g g c g t c a t t a a t c t t c g c g c c t t t t g g a c g a c t g t a

1200 LSDGKISEKTCCPGKFDTED QTIPFNAFLCTTRPFKIRFI t g a g t g a t g g g a a a a t t a g c g a a a a g a c a t g t t g t c c t g g g a a a t t t g a t a c g g a a g a t a a c t c a c t a c c c t t t t a a t c g c t t t t c t g t a c a a c a g g a c c c t t t a a a c t a t g c c t t c t a t

1260 KDRLLQLRQRSTLRWTDKNP FVPQQLQPLPGGKTPGILVG a a g a c a g g t t g c t g c a a t t g a g g c a g c g g t c c a c c c t t c g t t g g a c c g a t a a g a a c c c c t t t c t g t c c a a c g a c g t t a a c t c c g t c g c c a g g t g g g a a g c a a c c t g g c t a t t c t t g g g g a

1320 CPDNAETYCPFCELLGRSGN TWIVSFSVARKAFKEPSGTV g t c c a g a t a a c g c t g a a a c t t a c t g c c c g t t t t g c g a a c t t c t c g g g a g a t c c g g t a a c g c a g g t c t a t t g c g a c t t t g a a t g a c g g g c a a a a c g c t t g a a g a g c c c t c t a g g c c a t t g c

1380 DGKKAEKKDWRFRIHFGNLS ISLLSLLLIPAKTNMKAIKG a t g g a a a g a a g g c t g a g a a g a a g g a t t g g c g c t t t c g t a t t c a t t t t g g c a a t c t t t c c c t a c c t t t c t t c c g a c t c t t c t t c c t a a c c g c g a a a g c a t a a g t a a a a c c g t t a g a a a g g g

1440 LPGKPDFDGPKAIGSQRVLN KWPLGIEVPWFSYAALPYKV t t c c a g g g a a g c c c g a t t t c g a c g g g c c a a a a g c t a t a g g c a g c c a a c g g g t a c t t a a c a a a g g t c c c t t c g g g c t a a a g c t g c c c g g t t t t c g a t a t c c g t c g g t t g c c c a t g a a t t g t

1500 RVDFKSGKAHDFFKAYEVDH PDIEFGTLGMVEKLSVFDVM g g g t c g a t t t c a a a t c c g g t a a g g c c c a t g a c t t c t t t a a g g c t t a c g a a g t c g a c c a t a c c c a g c t a a a g t t t a g g c c a t t c c g g g t a c t g a a g a a a t t c c g a a t g c t t c a g c t g g t a t

1560 TRFPRFEGEITIDNKVSAEA c t a g g t t t c c c c g c t t c g a a g g g g a g a t t a c c a t a g a t a a t a a a g t c a g c g c t g a a g c c a g a t c c a a a g g g g c g a a g c t t c c c c t c t a a t g g t a t c t a t t a t t t c a g t c g c g a c t t c g g t

1620 RKLLCDSLKFTDRLCGALCV g g a a a c t g c t c t g t g a t t c t c t c a a g t t t a c g g a t c g g c t g t g t g g a g c t c t g t g c g t a a c c t t t g a c g a g a c a c t a a g a g a g t t c a a a t g c c t a g c c g a c a c a c c t c g a g a c a c g c a t t

1680 IRFDEYTPAADSGKQTENVQ t c a g g t t c g a t g a a t a t a c a c c a g c a g c c g a t a g t g g g a a a c a a a c c g a a a a t g t c c a a g a g t c c a a g c t a c t t a t a t g t g g t c g t c g g c t a t c a c c c t t t g t t t g g c t t t t a c a g g t t c

1740 AEPNANLAEKTAEQIISILD c a g a a c c g a a t g c t a a t c t c g c t g a a a a g a c c g c a g a g c a a a t t a t t a g c a t t c t g g a c g g t c t t g g c t t a c g a t t a g a g c g a c t t t t c t g g c g t c t c g t t t a a t a a t c g t a a g a c c t g c

1800 DNKKTEYTRLLADAIRSLRR a t a a t a a g a a a a c c g a a t a t a c c c g c c t g c t t g c t g a t g c t a t t c g t t c a c t c c g c c g c t t a t t a t t c t t t t g g c t t a t a t g g g c g g a c g a a c g a c t a c g a t a a g c a a g t g a g g c g g c g a

1860 SSKLVAGLPKDHDGKDDHYL c t a g t a a a c t t g t c g c t g g c t t g c c a a a g g a t c a t g a c g g g a a g g a t g a c c a t t a c c t c t g a t c a t t t g a a c a g c g a c c g a a c g g t t t c c t a g t a c t g c c c t t c c t a c t g g t a a t g g a g a

EcoRV 1920 WDIGKKKKDENSVTIRQILT g g g a t a t c g g c a a g a a g a a g a a a g a c g a a a a c t c t g t c a c t a t t a g g c a a a t c c t t a c g a c c c t a t a g c c g t t c t t c t t c t t t c t g c t t t t g a g a c a g t g a t a a t c c g t t t a g g a a t g c t

1980 TSADTKELKNAGKWREFCEK c c t c a g c a g a c a c c a a g g a a c t c a a g a a t g c c g g a a a a t g g a g a g a a t t c t g c g a g a a g c g g a g t c g t c t g t g g t t c c t t g a g t t c t t a c g g c c t t t t a c c t c t c t t a a g a c g c t c t t c g

2040 LGEALYLKSKDMSGGLKITR t g g g t g a a g c g c t g t a c c t c a a g a g t a a a g a c a t g a g t g g c g g c c t g a a a a t t a c t c g g a a c c c a c t t c g c g a c a t g g a g t t c t c a t t t c t g t a c t c a c c g c c g g a c t t t t a a t g a g c c t

2100 RILGDAEFHGKPDRLEKSRS g a a t a c t g g g c g a t g c t g a a t t c c a t g g a a a g c c c g a t c g g c t t g a a a a g a g c c g g t c t g c t t a t g a c c c g c t a c g a c t t a a g g t a c c t t t c g g g c t a g c c g a a c t t t t c t c g g c c a g a c

2160 VSIGSVLKETVVCGELVAKT t g t c a a t c g g a t c t g t g t t g a a a g a a a c t g t g g t a t g c g g c g a a c t g g t c g c t a a a a c g c a c a g t t a g c c t a g a c a c a a c t t t c t t t g a c a c c a t a c g c c g c t t g a c c a g c g a t t t t g c g

2220 PFFFGAIDEDAKQTDLQVLL c c t t c t t c t t c g g a g c g a t a g a c g a a g a t g c a a a a c a a a c c g a c c t g c a a g t a c t c c t c a g g a a g a a g a a g c c t c g c t a t c t g c t t c t a c g t t t t g t t t g g c t g g a c g t t c a t g a g g a g t

2280 TPDNKYRLPRSAVRGILRRD c t c c c g a t a a c a a g t a t a g a c t g c c a a g a a g c g c c g t g c g a g g t a t a c t c c g t c g a g a t c g a g g g c t a t t g t t c a t a t c t g a c g g t t c t t c g c g g c a c g c t c c a t a t g a g g c a g c t c t a g

2340 LQTYFDSPCNAELGGRPCMC t t c a a a c c t a t t t t g a t a g c c c a t g c a a t g c t g a g t t g g g t g g a c g g c c a t g c a t g t g t a a a g t t t g g a t a a a a c t a t c g g g t a c g t t a c g a c t c a a c c c a c c t g c c g g t a c g t a c a c a t

BssHIIBmgBI 2400 KTCRIMRGITVMDARSEYNA a g a c g t g t a g g a t t a t g a g a g g g a t c a c g g t g a t g g a t g c g c g c a g t g a g t a c a a t g c c c t c t g c a c a t c c t a a t a c t c t c c c t a g t g c c a c t a c c t a c g c g c g t c a c t c a t g t t a c g g g

2460 PPEIRHRTRINPFTGTVAEG c g c c a g a a a t a a g g c a t c g t a c c c g c a t t a a t c c c t t c a c a g g c a c g g t t g c g g a a g g t g g c g g t c t t t a t t c c g t a g c a t g g g c g t a a t t a g g g a a g t g t c c g t g c c a a c g c c t t c c a c

Acc65IKpnI 2520 ALFNMEVAPEGIVFPFQLRY c c c t g t t t a a t a t g g a a g t a g c c c c c g a g g g g a t t g t c t t t c c a t t t c a a c t c c g g t a c c g g g a c a a a t t a t a c c t t c a t c g g g g g c t c c c c t a a c a g a a a g g t a a a g t t g a g g c c a t g g

2580 RGSEDGLPDALKTVLKWWAE g g g g c t c t g a a g a t g g g c t g c c c g a t g c a c t g a a a a c g g t g t t g a a a t g g t g g g c t g a g g c c c c g a g a c t t c t a c c c g a c g g g c t a c g t g a c t t t t g c c a c a a c t t t a c c a c c c g a c t c c

2640 GQAFMSGAASTGKGRFRMEN g g c a g g c a t t c a t g a g t g g c g c t g c c t c a a c c g g g a a g g g c c g a t t c c g g a t g g a a a a t g c c g t c c g t a a g t a c t c a c c g c g a c g g a g t t g g c c c t t c c c g g c t a a g g c c t a c c t t t t a c

2700 AKYETLDLSDENQRNDYLKN c t a a a t a t g a a a c g c t g g a t c t g a g c g a c g a g a a t c a a a g g a a t g a c t a t c t t a a g a a t t g a t t t a t a c t t t g c g a c c t a g a c t c g c t g c t c t t a g t t t c c t t a c t g a t a g a a t t c t t a a

2760 WGWRDEKGLEELKKRLNSGL *TQ g g g g a t g g c g t g a c g a g a a g g g g c t c g a g g a a c t g a a g a a a c g a c t g a a c t c a g g t c t g c c c c c t a c c g c a c t g c t c t t c c c c g a g c t c c t t g a c t t c t t t g c t g a c t t g a g t c c a g a c g

2820 PEPGNYRDPKWHEINVSIEM WLGTIIPIWFPVLNVNGNLH c a g a g c c c g g t a a t t a t a g g g a t c c a a a a t g g c a c g a g a t t a a c g t t t c c a t t g a g a t g g g t c t c g g g c c a t t a a t a t c c c t a g g t t t t a c c g t g c t c t a a t t g c a a a g g t a a c t c t a c c

2880 ASPFINGDPIRAAVDKRGTD CARKNIAVWDACGHVFTTCI c a a g c c c t t t t a t t a a t g g c g a c c c a a t c c g c g c a g c c g t g g a c a a a c g t g g t a c a g a t g g t t c g g g a a a a t a a t t a c c g c t g g g t t a g g c g c g t c g g c a c c t g t t t g c a c c a t g t c t a c

2940 VVTFVKYKAEGEEAKPVCAY HNGENLIFSFPFLRFGYTCV t g g t t a c c t t c g t t a a g t a t a a a g c t g a a g g g g a a g a g g c g a a a c c c g t a t g t g c a t a c a a c c a a t g g a a g c a a t t c a t a t t t c g a c t t c c c c t t c t c c g c t t t g g g c a t a c a c g t a t g t

3000 KAESFRGVIRSAVARIHMED LGFRKSPHDSTGHCANMHFI a g g c c g a a t c t t t t a g a g g g g t g a t c a g a a g t g c c g t g g c a c g c a t t c a t a t g g a a g a t g t c c g g c t t a g a a a a t c t c c c c a c t a g t c t t c a c g g c a c c g t g c g t a a g t a t a c c t t c t a c

3060 GVPLTELTHSDCECLLCQIF ADRQSLQSVTVTFTEQALDK g c g t c c c t t t g a c t g a g t t g a c t c a c a g t g a c t g t g a a t g t c t c c t g t g c c a a a t c t t t g c g c a g g g a a a c t g a c t c a a c t g a g t g t c a c t g a c a c t t a c a g a g g a c a c g g t t t a g a a a c

3120 GSEYEAGKIRFEDLVFESDP STLIFGAFYPKFIEYEFTVR g a a g t g a g t a t g a a g c c g g c a a a a t a a g g t t t g a a g a t c t c g t a t t c g a a a g t g a c c c g g c t t c a c t c a t a c t t c g g c c g t t t t a t t c c a a a c t t c t a g a g c a t a a g c t t t c a c t g g g c c

MscI 3180 EPVTFDHVAIDRFTGGAADK FRHGEIMHGDISESATCSIL a a c c t g t g a c c t t c g a t c a t g t g g c c a t c g a t a g a t t c a c t g g c g g t g c a g c t g a t a a g a t t g g a c a c t g g a a g c t a g t a c a c c g g t a g c t a t c t a a g t g a c c g c c a c g t c g a c t a t t c t

3240 KKFDDSPLPGSPARPLMLKG LFEIIGRQGTARCSREHQFA a g a a a t t c g a t g a t t c c c c t c t g c c c g g t a g c c c t g c a a g a c c g c t c a t g t t g a a a g g c t t c t t t a a g c t a c t a a g g g g a g a c g g g c c a t c g g g a c g t t c t g g c g a g t a c a a c t t t c c g a

3300 SFWIRRDVLEDEEYCKALGK GEPDAPVNELVFLVTLCETL c c t t c t g g a t c c g c a g g g a c g t t c t c g a g g a c g a a g a g t a c t g t a a g g c a c t c g g t a a g g g g a a g a c c t a g g c g t c c c t g c a a g a g c t c c t g c t t c t c a t g a c a t t c c g t g a g c c a t t c c

3360 ALADVNNGLYPLGGKSAIGY SKCIHIIAKIGETSLAGDAV c t c t t g c a g a t g t g a a t a a t g g c c t t t a t c c c c t c g g t g g a a a g a g c g c c a t c g g c t a c g g a g a a c g t c t a c a c t t a t t a c c g g a a a t a g g g g a g c c a c c t t t c t c g c g g t a g c c g a t g c

3420 GQVKSLGIKGDDKRISRLMN SLDLTQTYLSIILPNRAEHI g a c a g g t c a a g a g t c t g g g t a t a a a g g g a g a t g a t a a g a g g a t t t c t c g c c t c a t g a a t c c t g t c c a g t t c t c a g a c c c a t a t t t c c c t c t a c t a t t c t c c t a a a g a g c g g a g t a c t t a g

3480 PAFDETDVAVPEKPKTDAEV RGKILCIYGNWFLGFSIGLN c t g c c t t t g a t g a g a c a g a t g t a g c c g t t c c a g a a a a g c c c a a a a c t g a t g c c g a g g t t c g a c g g a a a c t a c t c t g t c t a c a t c g g c a a g g t c t t t t c g g g t t t t g a c t a c g g c t c c a a g

3540 RIEAEKVYYPHYFVEPHKKV ADLCLFYIVWVIEDFGMLLH g c a t c g a g g c a g a g a a a g t a t a t t a c c c a c a c t a t t t c g t c g a a c c c c a t a a g a a g g t g g c g t a g c t c c g t c t c t t t c a t a t a a t g g g t g t g a t a a a g c a g c t t g g g g t a t t c t t c c a c c

3600 EREEKPCGHQKFHEGRLTGK FALLFGTTMLLEVLPSQCTF a a c g c g a g g a g a a a c c c t g t g g t c a t c a a a a g t t c c a c g a g g g g c g a c t g a c a g g t a a a a t t g c g c t c c t c t t t g g g a c a c c a g t a g t t t t c a a g g t g c t c c c c g c t g a c t g t c c a t t t t

3660 IRCKLITKTPLIVPDTSNDD NPTLENGLCGEDDWISTIVI t t c g g t g t a a g c t c a t t a c c a a g a c a c c c c t c a t c g t c c c a g a t a c t a g t a a t g a c g a t t a a g c c a c a t t c g a g t a a t g g t t c t g t g g g g a g t a g c a g g g t c t a t g a t c a t t a c t g c t a a

3720 FFRPADKEARKEKDEYHKSY EESRRIFFSPLFLVFIM t c t t c a g a c c t g c g g a t a a a g a a g c t c g g a a g g a a a a g g a c g a a t a t c a t a a a t c a t a t g a g a a g t c t g g a c g c c t a t t t c t t c g a g c c t t c c t t t t c c t g c t t a t a g t a t t t a g t a t a c

3780 AFFRLHKQIMIPGSELRGMV c t t t c t t c a g a c t t c a t a a a c a a a t c a t g a t t c c c g g g a g c g a a t t g a g a g g a a t g g t g a g a a a g a a g t c t g a a g t a t t t g t t t a g t a c t a a g g g c c c t c g c t t a a c t c t c c t t a c c a c t

3840 SSVYETVTNSCFRIFDETKR g t a g t g t c t a c g a a a c t g t g a c a a a t t c t t g c t t c a g g a t a t t t g a t g a g a c t a a a c g g t c a t c a c a g a t g c t t t g a c a c t g t t t a a g a a c g a a g t c c t a t a a a c t a c t c t g a t t t g c c a

3900 LSWRMDADHQNVLQDFLPGR *PPHISIVLIHQLVKEWTS t g t c a t g g c g g a t g g a t g c t g a t c a c c a g a a t g t g c t g c a a g a c t t t c t c c c a g g t c g a g a c a g t a c c g c c t a c c t a c g a c t a g t g g t c t t a c a c g a c g t t c t g a a a g a g g g t c c a g c t c

3960 VTADGKHIQKFSETARVPFY HGGIPFMYLLKGFSCPHREI t g a c c g c c g a t g g g a a a c a t a t a c a a a a g t t t t c c g a a a c t g c a a g g g t g c c t t t c t a t g a c t g g c g g c t a c c c t t t g t a t a t g t t t t c a a a a g g c t t t g a c g t t c c c a c g g a a a g a t a c

4020 DKTQKHFDILDEQEIAGEKP VFRLFMKVNKILLFNSTLFR a c a a a a c g c a g a a a c a t t t t g a c a t t c t t g a t g a g c a a g a a a t t g c t g g t g a g a a a c c t g t g t t t t g c g t c t t t g t a a a a c t g t a a g a a c t a c t c g t t c t t t a a c g a c c a c t c t t t g g a c

4080 VRMWVKRFIKRLSLVDPAKH NPHPDLTKNFSQAENIRSFV t t c g g a t g t g g g t c a a g c g t t t t a t t a a a c g a c t g a g c c t c g t t g a t c c t g c t a a a c a c c a a g c c t a c a c c c a g t t c g c a a a a t a a t t t g c t g a c t c g g a g c a a c t a g g a c g a t t t g t g g

4140 PQKKQDNKWKRRKEGIATFI GLLFLVIFPFSAFFADGCKY c c c a g a a g a a a c a a g a c a a t a a a t g g a a a a g a c g c a a a g a a g g c a t c g c c a c a t t t a t a g g g g t c t t c t t t g t t c t g t t a t t t a c c t t t t c t g c g t t t c t t c c g t a g c g g t g t a a a t a t c

4200 EQKNGSYYFNVVTNNGCTSF LLLIARIIKVHDGIIAASRE a g c a a a a g a a t g g c t c t t a t t a t t t t a a c g t g g t c a c c a a t a a t g g c t g c a c t t c t t t c c t c g t t t t c t t a c c g a g a a t a a t a a a a t t g c a c c a g t g g t t a t t a c c g a c g t g a a g a a a g g

4260 HLWHKPDNFDQEKLEGIQNG VQPMFRVIKVLLFEFAYLIT a c t t g t g g c a t a a a c c t g a c a a t t t t g a c c a g g a g a a a c t c g a a g g c a t a c a g a a t g g t g t g a a c a c c g t a t t t g g a c t g t t a a a a c t g g t c c t c t t t g a g c t t c c g t a t g t c t t a c c a c

StuI 4320 EKLDCWVRDSRYQKAFQEIP LFQIAPYSITSVLLGKLLYG a g a a a c t g g a t t g c t g g g t a a g a g a t a g t a g a t a c c a a a a g g c c t t t c a a g a g a t a c c c g t c t t t g a c c t a a c g a c c c a t t c t c t a t c a t c t a t g g t t t t c c g g a a a g t t c t c t a t g g g c

4380 ENDPDGWECKEGYLHVVGPS LVVWVSPLTFLAVKM a g a a c g a c c c a g a c g g a t g g g a g t g t a a a g a g g g c t a c c t t c a t g t c g t c g g c c c c a g c a t c t t g c t g g g t c t g c c t a c c c t c a c a t t t c t c c c g a t g g a a g t a c a g c a g c c g g g g t c g t

4440 KVEFSDKKGDVINNFQGTLP a a g t a g a g t t t a g t g a c a a g a a a g g g g a t g t g a t c a a t a a c t t t c a a g g a a c a c t c c c a t t t c a t c t c a a a t c a c t g t t c t t t c c c c t a c a c t a g t t a t t g a a a g t t c c t t g t g a g g g t a

4500 SVPNDWKTIRTNDFKNRKRK c a g t t c c c a a c g a c t g g a a a a c a a t t a g g a c g a a c g a t t t t a a g a a t a g g a a a a g g a a g a g t c a a g g g t t g c t g a c c t t t t g t t a a t c c t g c t t g c t a a a a t t c t t a t c c t t t t c c t t c t

4560 NEPVFCCEDDKGNYYTMAKY a t g a a c c t g t g t t t t g t t g t g a g g a t g a t a a g g g c a a t t a c t a t a c c a t g g c t a a a t a t t t a c t t g g a c a c a a a a c a a c a c t c c t a c t a t t c c c g t t a a t g a t a t g g t a c c g a t t t a t a a

4620 CETFFFDLKENEEYEIPEKA g c g a a a c c t t c t t c t t c g a t c t g a a g g a g a a c g a g g a a t a c g a g a t c c c c g a g a a a g c a a c g c t t t g g a a g a a g a a g c t a g a c t t c c t c t t g c t c c t t a t g c t c t a g g g g c t c t t t c g t t

4680 RIKYKELLRVYNNNPQAVPE g a a t c a a a t a c a a a g a a c t g c t c a g g g t c t a t a a c a a c a a t c c t c a a g c a g t g c c g g a g a c t t a g t t t a t g t t t c t t g a c g a g t c c c a g a t a t t g t t g t t a g g a g t t c g t c a c g g c c t c t

XbaI 4740 SVFQSRVARENVEKLKSGDL g c g t a t t t c a g t c t a g a g t t g c c c g g g a a a a c g t g g a a a a g c t g a a g t c c g g a g a t c t t g c g c a t a a a g t c a g a t c t c a a c g g g c c c t t t t g c a c c t t t t c g a c t t c a g g c c t c t a g a a c

Tth111IPflFI 4800 VYFKHNEKYVEDIVPVRISR t g t a t t t c a a a c a t a a t g a a a a g t a c g t a g a g g a c a t c g t c c c a g t g c g g a t t t c c c g a a a c a t a a a g t t t g t a t t a c t t t t c a t g c a t c t c c t g t a g c a g g g t c a c g c c t a a a g g g c t t

4860 TVDDRMIGKRMSADLRPCHG c t g t a g a c g a t a g g a t g a t c g g c a a a c g t a t g a g c g c c g a t c t g c g g c c g t g c c a t g g a g g a c a t c t g c t a t c c t a c t a g c c g t t t g c a t a c t c g c g g c t a g a c g c c g g c a c g g t a c c t c

4920 DWVEDGDLSALNAYPEKRLL a t t g g g t g g a a g a t g g t g a t c t c a g t g c c t t g a a t g c a t a t c c c g a g a a a a g a c t c c t c t t a a c c c a c c t t c t a c c a c t a g a g t c a c g g a a c t t a c g t a t a g g g c t c t t t t c t g a g g a g a

4980 LRHPKGLCPACRLFGTGSYK t g c g c c a c c c c a a a g g a c t c t g c c c t g c t t g c c g g c t c t t t g g a a c c g g a t c t t a c a a g g a c g c g g t g g g g t t t c c t g a g a c g g g a c g a a c g g c c g a g a a a c c t t g g c c t a g a a t g t t c c

5040 GRVRFGFASLENDPEWLIPG g c a g a g t c a g g t t t g g a t t c g c g t c a c t c g a a a a c g a t c c g g a g t g g c t g a t c c c a g g c a c g t c t c a g t c c a a a c c t a a g c g c a g t g a g c t t t t g c t a g g c c t c a c c g a c t a g g g t c c g t

5100 KNPGDPFHGGPVMLSLLERP a g a a t c c c g g c g a t c c g t t t c a c g g c g g g c c g g t g a t g c t c t c a t t g t t g g a a c g g c c t c t c t t a g g g c c g c t a g g c a a a g t g c c g c c c g g c c a c t a c g a g a g t a a c a a c c t t g c c g g a g

5160 RPTWSIPGSDNKFKVPGRKF g c c c g a c t t g g a g t a t a c c g g g a t c c g a c a a t a a g t t t a a a g t g c c t g g c a g a a a g t t t t c g g g c t g a a c c t c a t a t g g c c c t a g g c t g t t a t t c a a a t t t c a c g g a c c g t c t t t c a a a a

5220 YVHHHAWKTIKDGNHPTTGK a c g t c c a c c a c c a c g c c t g g a a a a c c a t t a a g g a c g g g a a c c a t c c c a c a a c a g g c a a a g t g c a g g t g g t g g t g c g g a c c t t t t g g t a a t t c c t g c c c t t g g t a g g g t g t t g t c c g t t t c

SgrAIMreI 5280 AIEQSPNNRTVEALAGGNSF c t a t t g a a c a a a g c c c t a a t a a c c g c a c t g t a g a a g c t c t c g c c g g c g g g a a t t c c t t t a g a t a a c t t g t t t c g g g a t t a t t g g c g t g a c a t c t t c g a g a g c g g c c g c c c t t a a g g a a a t

5340 SFEIAFENLKEWELGLLIHS g c t t c g a a a t t g c c t t t g a g a a c c t g a a a g a a t g g g a g c t g g g t t t g c t c a t c c a c a g c c c g a a g c t t t a a c g g a a a c t c t t g g a c t t t c t t a c c c t c g a c c c a a a c g a g t a g g t g t c g g

5400 LQLEKGLAHKLGMAKSMGFG t g c a a c t c g a a a a g g g t c t g g c g c a t a a a c t t g g a a t g g c a a a g t c t a t g g g a t t t g g t t a c g t t g a g c t t t t c c c a g a c c g c g t a t t t g a a c c t t a c c g t t t c a g a t a c c c t a a a c c a a

5460 SVEIDVESVRLRKDWKQWRN c a g t t g a a a t t g a c g t c g a a t c a g t g c g c c t g a g a a a a g a t t g g a a g c a a t g g c g g a a t g g t c a a c t t t a a c t g c a g c t t a g t c a c g c g g a c t c t t t t c t a a c c t t c g t t a c c g c c t t a c

PfoI 5520 GNSEIPNWLGKGFAKLKEWF g c a a t t c c g a a a t t c c c a a c t g g t t g g g a a a a g g a t t t g c t a a a c t g a a g g a a t g g t t c c c g t t a a g g c t t t a a g g g t t g a c c a a c c c t t t t c c t a a a c g a t t t g a c t t c c t t a c c a a g g

Bsu36I 5580 RDELDFIENLKKLLWFPEGD g g g a c g a g c t c g a t t t t a t a g a a a a t c t t a a g a a a c t t c t t t g g t t t c c t g a g g g c g a c c c c c t g c t c g a g c t a a a a t a t c t t t t a g a a t t c t t t g a a g a a a c c a a a g g a c t c c c g c t g g

5640 QAPRVCYPMLRKKDDPNGNS a a g c a c c c c g g g t t t g c t a c c c c a t g c t g c g a a a g a a g g a c g a t c c t a a t g g g a a t a g c g t t c g t g g g g c c c a a a c g a t g g g g t a c g a c g c t t t c t t c c t g c t a g g a t t a c c c t t a t c g c

5700 GYEELKDGEFKKEDRQKKLT g t t a c g a a g a a c t c a a a g a c g g g g a a t t c a a g a a a g a a g a t c g g c a g a a g a a g c t g a c c a c a a t g c t t c t t g a g t t t c t g c c c c t t a a g t t c t t t c t t c t a g c c g t c t t c t t c g a c t g g t

PmeIAgeI 5760 1 5 HHHHHH * 6xHis TPWTPWA* c g c c g t g g a c a c c g t g g g c a t a a c c g g t c a t c a t c a c c a t c a c c a t t g a g t t t a a a c c c g g c g g c a c c t g t g g c a c c c g t a t t g g c c a g t a g t a g t g g t a g t g g t a a c t c a a a t t t g g g c

5820 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a

5880 bGH poly(A) signal g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g a a a a t c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c t t t t a

5940 bGH poly(A) signal t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c

SphIBbsI 6000 bGH poly(A) signal c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g

6060 t t c t g a g g c g g a a a g a a c c a g c t g g g g c t c g a t a c c g t c g a c c t c t a g c t a g a g c t t g g c a a g a c t c c g c c t t t c t t g g t c g a c c c c g a g c t a t g g c a g c t g g a g a t c g a t c t c g a a c c g

6120 M13 rev lac operator lac promoter C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t

6180 -10 -35 lac promoter CAP binding site c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t a g g g t g c c t a a t g a g t g a g c t a a c t c a c g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a t c c c a c g g a t t a c t c a c t c g a t t g a g t g

6240 CAP binding site a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t

BspQISapI 6300 t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g

6360 G A G C G A G T G A C T G A G C G A L4440 c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g

6420 a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g

6480 ori a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c

DrdI 6540 ori g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g

6600 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a

6660 ori t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a

6720 ori t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c

6780 ori c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a

6840 ori t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a

6900 ori t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c

6960 ori c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t

7020 ori a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a

7080 ori t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g

7140 t a c g g g g t c t g a c a c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t g c c c c a g a c t g t g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a

7200 a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t

7260 285 280 *WHKILSAGI AmpR *WHKILSAGI a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a

7320 275 270 265 260 EAIQRNREDMTAQSGTTYIV AmpR EAIQRNREDMTAQSGTTYIV c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g

BpmIBsaI 7380 255 250 245 240 VIRSPKGDPGLAAIIGRSGR AmpR VIRSPKGDPGLAAIIGRSGR t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c a c g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g t g c

7440 235 230 225 220 EGAGSKDAIFWGAPLASRLL AmpR EGAGSKDAIFWGAPLASRLL c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c

7500 215 210 205 200 PGAVKDAEMWDILQQRSALT AmpR PGAVKDAEMWDILQQRSALT t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a

FspI 7560 195 190 185 180 LLEGTLLKRLTTAMAVPMTT AmpR LLEGTLLKRLTTAMAVPMTT a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a

7620 175 170 165 160 DREDNPIAENLEPEWRDLRT AmpR DREDNPIAENLEPEWRDLRT g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a

7680 155 150 145 140 VHDGMNHLFATLEKPGGITT AmpR VHDGMNHLFATLEKPGGITT t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a

7740 135 130 125 120 LLLNAATNDSMTIAASCLER AmpR LLLNAATNDSMTIAASCLER c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a

7800 115 110 105 100 VTMGDTLHKETVPSYEVLDN AmpR VTMGDTLHKETVPSYEVLDN t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a

7860 95 90 85 80 QSYHIRRGLQEQGADIRSLV AmpR A T A A T A C Amp-R QSYHIRRGLQEQGADIRSLV c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g

7920 75 70 65 60 AGCLLVKFTSMMPFREEPRF AmpR C G C G C C A C A T A G C Amp-R AGCLLVKFTSMMPFREEPRF c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t

7980 55 50 45 40 SELIKGSNLDLEIYGVRAGL AmpR SELIKGSNLDLEIYGVRAGL a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t

8040 35 30 25 QDEADKVKVLTEPH 20 signal sequence AFVPLC AmpR QDEADKVKVLTEPHAFVPLC c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t

8100 15 10 5 1 signal sequence FAAFFPILAVRFHQISM AmpR AmpR promoter FAAFFPILAVRFHQISM a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a

8160 AmpR promoter t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t

3ʹ 5ʹ 8205 AmpR promoter a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.