Addgene pGL3-noSV40-mouseEC1.45 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pGL3-noSV40-mouseEC1.45 Sequencing Result


Map View

Acc65I (1) RVprimer3 (8508 .. 8527) NotI (8399) F1ori-F (8117 .. 8138) DraIII (8053) BsaAI (8050) F1ori-R (7907 .. 7926) Amp-R (7451 .. 7470) pBR322ori-F (6149 .. 6168) L4440 (5898 .. 5915) AfeI (5884) PshAI (5823) BamHI (5752) HpaI (5644) SV40pA-R (5588 .. 5607) EBV-rev (5534 .. 5553) FseI (5503) XbaI (5484) SgrAI (5258) XcmI (4565) BstBI (3999) KpnI (5) EcoRV (366) NheI (396) BmtI (400) KflI (1478) SgrDI (2390) PacI (2640) SbfI (3235) PaeR7I - XhoI (3582) SfiI (3732) StuI (3778) AvrII (3779) HindIII (3795) KasI (3862) NarI (3863) SfoI (3864) PluTI (3866) LucNrev (3896 .. 3914) pGL3-noSV40-mouseEC1.45 8566 bp

Sequence View

Acc65IKpnI 5ʹ 3ʹ 60 g g t a c c t c c a t a a c a t c c c c c a a a t g g c a c c a a c a a c c a a g t g t t c t a a t a c c c g a g t c t c c a t g g a g g t a t t g t a g g g g g t t t a c c g t g g t t g t t g g t t c a c a a g a t t a t g g g c t c a g a

120 g t g t g g g a c a t t t c t c a t t c a a a c c a c c a c a g t a a g t a a a t t a a a t c a t g c g a a t g a c t t c a c a c c c t g t a a a g a g t a a g t t t g g t g g t g t c a t t c a t t t a a t t t a g t a c g c t t a c t g a a

180 t t g t a a a c t a c g t t g t g t t g g g t a a c g t a a c t g c a a c a t a g c c t a g t t t c t a t t t c a c a t a a c a t t t g a t g c a a c a c a a c c c a t t g c a t t g a c g t t g t a t c g g a t c a a a g a t a a a g t g t a

240 c a c c a a c a t g a t t c c a t c t g a t a a c g t t c a c a t a g c a t g a t a g c t t c t c t a c t c a t t t c a g t g g t t g t a c t a a g g t a g a c t a t t g c a a g t g t a t c g t a c t a t c g a a g a g a t g a g t a a a g t

300 a g g a a g t t c a a a g c a t g t t g a a t t t a a c t c t a g c a t g t a a a t a g t g c a c t c t t c t g t a a t t c c t t c a a g t t t c g t a c a a c t t a a a t t g a g a t c g t a c a t t t a t c a c g t g a g a a g a c a t t a

360 c c t t t a g a a t a a t a c t a c a g t g c c a t t g t t a t t t a a t g t t a a t t a c a t c a t t t c c t t c t t g g a a a t c t t a t t a t g a t g t c a c g g t a a c a a t a a a t t a c a a t t a a t g t a g t a a a g g a a g a a

BmtINheIEcoRV 420 t c t g a t a t c a a c t c t a c t t a a t c c t g t c t t a c g c c g c t a g c c a g t g c c t g g a g g t g a c t g a g a c t a t a g t t g a g a t g a a t t a g g a c a g a a t g c g g c g a t c g g t c a c g g a c c t c c a c t g a c

480 g c a t t g t g t t t t g c c c a g a a g t t a t a g a t g c t a c t t a t g a g a g g g t g a g t g c t t g t c c a a c g t a a c a c a a a a c g g g t c t t c a a t a t c t a c g a t g a a t a c t c t c c c a c t c a c g a a c a g g t t

540 a t g a a g t t t g t c a c t c a g a g c t g g a a a t t g a a g c a t a a a g a t c a a t a c c a a a a c c t c c c a t a c t t c a a a c a g t g a g t c t c g a c c t t t a a c t t c g t a t t t c t a g t t a t g g t t t t g g a g g g t

600 g c c a c t g c t c c a g a a t a c a g t g g a g t t t c t t c t g c t c c c t g t t t t t a t t t a t g g t g t g t a c g g t g a c g a g g t c t t a t g t c a c c t c a a a g a a g a c g a g g g a c a a a a a t a a a t a c c a c a c a t

660 c t c a t g g a g a a a c a a g c c a g t t c c c c c c c a g g a c c c a g t g a a g a g c a t t t a c t c a g t a t t g a g t a c c t c t t t g t t c g g t c a a g g g g g g g t c c t g g g t c a c t t c t c g t a a a t g a g t c a t a a

720 a t t g g a g c a a t g g c a t c g t t t t t c a g a t a t a t c c a a g t t c t g t t c c t t g a t c t g t g a c c a t a a c c t c g t t a c c g t a g c a a a a a g t c t a t a t a g g t t c a a g a c a a g g a a c t a g a c a c t g g t

780 t t a t a t t t a c c c a a a a t a t c c t t c t c a t g g a a a a c a g t t c t c t g g a c t c t g c t t g a g a c t a a t a t a a a t g g g t t t t a t a g g a a g a g t a c c t t t t g t c a a g a g a c c t g a g a c g a a c t c t g a

840 c a t a g g c t c a g t c a t c t g c t t c a c t t t c t c a g a a g a g a a g t c a c a t a g t t a c a c t a a t t c g t a t c c g a g t c a g t a g a c g a a g t g a a a g a g t c t t c t c t t c a g t g t a t c a a t g t g a t t a a g

900 t t g c t g c t t c t t g g c a c c c c c a a a t t c t c t t a g c t a a c g a g a g c t t g t c t t g g g a c c a g g a a c g a c g a a g a a c c g t g g g g g t t t a a g a g a a t c g a t t g c t c t c g a a c a g a a c c c t g g t c c

960 c t c t t g g g a g c t t t g g a g a c c a t t t c a t c c a c a g a g c t t t g g a a t t c c c t c c t c a c c c g a g a g a a c c c t c g a a a c c t c t g g t a a a g t a g g t g t c t c g a a a c c t t a a g g g a g g a g t g g g c t

1020 c a t g t g c a g c t t a t a c a t c a a a c t t g t g g t t t g a g a c a t t t t t t t t t t c a g a t g c c t g a a g t a c a c g t c g a a t a t g t a g t t t g a a c a c c a a a c t c t g t a a a a a a a a a a g t c t a c g g a c t t

1080 g c a t c a a c a g c a c t c t t a c t c t a c c a g c a t t a a c t t a t t a c c a t t t c a g t g g c a t t t g a a c g t a g t t g t c g t g a g a a t g a g a t g g t c g t a a t t g a a t a a t g g t a a a g t c a c c g t a a a c t t

1140 g a a g a a a a g g a g a t a a a t a c a t a c t t g a a a g t g c t g c t a c c a a t g t t t t t t t t c t a c a c a c t t c t t t t c c t c t a t t t a t g t a t g a a c t t t c a c g a c g a t g g t t a c a a a a a a a a g a t g t g t

1200 t t t g a t t g t a t t t g g t g c a a t t a t c g g c a t g t a t c t c c a c t t g c c a a g t a t a a g a g a g g g a a a c t a a c a t a a a c c a c g t t a a t a g c c g t a c a t a g a g g t g a a c g g t t c a t a t t c t c t c c c

1260 t t a g g g g t g t g t g t g t g t g t g t g t g t g t g t c c a t c c a t c t g t c t g t g t t t g t g t g t g a t c a a t c c c c a c a c a c a c a c a c a c a c a c a c a c a g g t a g g t a g a c a g a c a c a a a c a c a c a c t a g

1320 a a a g a t c t t t g g g g g c c a t g c c c t c a t t a t c t t t c a g c c c a g c a t c t t t g a t t a a g a a c a t t t c t a g a a a c c c c c g g t a c g g g a g t a a t a g a a a g t c g g g t c g t a g a a a c t a a t t c t t g t

1380 g g t g c a g c c a a g c t c t g g c c t t g c c t c c c a t t t c t g c c g g c a t c a a a t a a a a a g c a g a a g c c a c g t c g g t t c g a g a c c g g a a c g g a g g g t a a a g a c g g c c g t a g t t t a t t t t t c g t c t t c

1440 t a t g a c t g t t t g t t g g a t c t t t c c c t t a c t t t t a a t t c t c t g g t g c t g a a g a a c a t g c c a a t a c t g a c a a a c a a c c t a g a a a g g g a a t g a a a a t t a a g a g a c c a c g a c t t c t t g t a c g g t

KflI 1500 a g t c a t g g g c a a g a a t c t a a a a t t g t g g c t g a c c a a g g g t c c c a c a t c c c g g c a t g a t c c t c a g t a c c c g t t c t t a g a t t t t a a c a c c g a c t g g t t c c c a g g g t g t a g g g c c g t a c t a g g

1560 c c a c a g c t c t g c a g t t g t a a g a t a g g a g g t t t t g a t g t g g t t c t g t t g c a c t t c t a t c t c g g t g t c g a g a c g t c a a c a t t c t a t c c t c c a a a a c t a c a c c a a g a c a a c g t g a a g a t a g a g

1620 c c a t g t a a t t t c a a t t t a a t c a g c t t c c g t a c a t g c t a t a a a t t a t g g c t t c c c c g a t c t g g t a c a t t a a a g t t a a a t t a g t c g a a g g c a t g t a c g a t a t t t a a t a c c g a a g g g g c t a g a

1680 a t c c t t t t t a t g t g t a t g c g g t g a t g t a a t t t t c t t t t t g a g a t t t c t a t a c t t g g a t g a t a g g a a a a a t a c a c a t a c g c c a c t a c a t t a a a a g a a a a a c t c t a a a g a t a t g a a c c t a c t

1740 c a g c a t c c t c t t a t t t c t t a a c c a g a t a c a a a t g c t c c t g a g t c g g t t c t t t g t g t a c t c g t c g t a g g a g a a t a a a g a a t t g g t c t a t g t t t a c g a g g a c t c a g c c a a g a a a c a c a t g a g

1800 c g a g g a c t c t g a g a t t g a t t g t g a g t t a a a t c a t g g a c c c c g a t g c c c a c t t c a c a c a g g g c t c c t g a g a c t c t a a c t a a c a c t c a a t t t a g t a c c t g g g g c t a c g g g t g a a g t g t g t c c

1860 t c a g a t c t t t c a c a t a a t t a a t a a c a t g c t a t c t a c t c t a t a a t t g g g c t c c a c a g a a g c a g t c t a g a a a g t g t a t t a a t t a t t g t a c g a t a g a t g a g a t a t t a a c c c g a g g t g t c t t c g

1920 a t g g t c a c t g t g a t a c t g t t t g a c c c t c a a t c a c a g c c t a g c t t a t a g a t a g c t c t a t t t t a c c a g t g a c a c t a t g a c a a a c t g g g a g t t a g t g t c g g a t c g a a t a t c t a t c g a g a t a a a

1980 t c c c c a t g t g c a a t g c t t t t g g t g g a t t c t g g g a t a t t a t a t t g t t g g a g t t t c g g t g a g a g g g g t a c a c g t t a c g a a a a c c a c c t a a g a c c c t a t a a t a t a a c a a c c t c a a a g c c a c t c

2040 c c c a g c t a t c t g a g g a c a c t t t a c t t t g a c a c a t g c c a c t t t t t a g a g g a g a g c t g g a a a g g g t c g a t a g a c t c c t g t g a a a t g a a a c t g t g t a c g g t g a a a a a t c t c c t c t c g a c c t t t

2100 t g t t c g t t a a a t g t g a g c c a a a t g g t t a g g g c a g a a a a g g c a a a a c c a a a g c t a a a t c t t a c a a g c a a t t t a c a c t c g g t t t a c c a a t c c c g t c t t t t c c g t t t t g g t t t c g a t t t a g a a

2160 t t t t t t t t t t c t t t t t c t t t t t t a a a t t t a a c a t a a a t a g a g t t g a t t c t a g g g a g a t a a a a a a a a a a a a g a a a a a g a a a a a a t t t a a a t t g t a t t t a t c t c a a c t a a g a t c c c t c t a t t

2220 a t g t a t t t t t a a t g a t g g g a g a a t t c t g g g t t c g t a c a t a a a t c a a g g c a t c t t g a g t t t t a c a t a a a a a t t a c t a c c c t c t t a a g a c c c a a g c a t g t a t t t a g t t c c g t a g a a c t c a a a

2280 a g a g a a a c a g a a a a t g a g t a a t a t c a t a t t t a g c t g t c t t c a c c a g a g a g t c c t a a t t c a t c t c t t t g t c t t t t a c t c a t t a t a g t a t a a a t c g a c a g a a g t g g t c t c t c a g g a t t a a g t

2340 a a a g t a t g t c a a a a g t g g c c a g g a g t a g c t c t g a g t g a a a g a a t c a t g g a a g t g c a a c a g t t t c a t a c a g t t t t c a c c g g t c c t c a t c g a g a c t c a c t t t c t t a g t a c c t t c a c g t t g t c

SgrDI 2400 a c a g a a a c c g g a g a a t g g g a a a t g a g a g t c g a a g a g g t t c c a g a a t a a c g t c g a c g g g a g t g t c t t t g g c c t c t t a c c c t t t a c t c t c a g c t t c t c c a a g g t c t t a t t g c a g c t g c c c t c

2460 c c a a t g c a c a g a t c t g t g t g c c a g g a a a g a t c g g c c c c a t g g a g a g a g a a c t g g a t c t g a g g t t a c g t g t c t a g a c a c a c g g t c c t t t c t a g c c g g g g t a c c t c t c t c t t g a c c t a g a c t

2520 a t a a a g g a g c t a t c a g t a c c a g g a c t t a c a t a g a c a g a g c a t t a t g a a g g a a g g a t a c a c t a t t t c c t c g a t a g t c a t g g t c c t g a a t g t a t c t g t c t c g t a a t a c t t c c t t c c t a t g t g

2580 a a c c c t t c c a t g c t c c c t g g g a c t g c t t g g c c t t a a t a a g c t a c a a a g a a a a t t a c a a a g t t g g g a a g g t a c g a g g g a c c c t g a c g a a c c g g a a t t a t t c g a t g t t t c t t t t a a t g t t t c

PacI 2640 a t g a c c a g c c a a a g g a a a a a c t c c t t t g c a g a c t g t a g g g g t t a t t a a t g a a g a t t t a a t t a c t g g t c g g t t t c c t t t t t g a g g a a a c g t c t g a c a t c c c c a a t a a t t a c t t c t a a a t t a

2700 t a a a g a t t a g t a c t t g a a t t g g c t c a t g g a c a g g t a g a a g a a t a t a g a t t t t t t t t t a a a a t t t c t a a t c a t g a a c t t a a c c g a g t a c c t g t c c a t c t t c t t a t a t c t a a a a a a a a a t t t

2760 c t g g c t t g a a c t c a g a t g a a a t g t g t t c a t c t t a a t t t t g t a t g a c a c t c a c t a c a a a a t g a c c g a a c t t g a g t c t a c t t t a c a c a a g t a g a a t t a a a a c a t a c t g t g a g t g a t g t t t t a

2820 c t c c a a a c t a c t a t c g g t a g a a g t t a g g a g a a a t t c c c a t a a a t a a c a t c t t c t c g g g a g g a g g t t t g a t g a t a g c c a t c t t c a a t c c t c t t t a a g g g t a t t t a t t g t a g a a g a g c c c t c

2880 a t c a g t a g a g a a t t t t c a t t g g a a a g t g a g g a a c a a a c c a a c a a a c a g a c a t g a a c t t t c t a g t c a t c t c t t a a a a g t a a c c t t t c a c t c c t t g t t t g g t t g t t t g t c t g t a c t t g a a a g

2940 c t c g a t t g t a t t g c a t t t t a a a a g a a a g g a t t c c a g g a a a c c t t c a g g a g a c a c c g c a t g g a g c t a a c a t a a c g t a a a a t t t t c t t t c c t a a g g t c c t t t g g a a g t c c t c t g t g g c g t a c

3000 c c t t c t g g g g c c a g g c a g c t g g g a g a g a g a g a g g g a a g a g a t t a a c g t a g a a c a g g t c g g g g a a g a c c c c g g t c c g t c g a c c c t c t c t c t c t c c c t t c t c t a a t t g c a t c t t g t c c a g c c

3060 g g a c c a t c c c a a g c c t a t c c t t c a g g c c c t c g a t t g a a g g a a t t a a g t t t t a t t t t a t t a c c t g g t a g g g t t c g g a t a g g a a g t c c g g g a g c t a a c t t c c t t a a t t c a a a a t a a a a t a a t

3120 a c t t c g t t c t t g c c c t c c c a g a g t t g a g g g t g c t c a c t c c a g g g c t c c c g t a g a a c c g c c t g a a g c a a g a a c g g g a g g g t c t c a a c t c c c a c g a g t g a g g t c c c g a g g g c a t c t t g g c g g

3180 c c a g g g a a c c t c a g t g c a a a a g g t t t g c a c t c a c a g c t g g c t g t g a t t a c t a g g a c t g g g g g t c c c t t g g a g t c a c g t t t t c c a a a c g t g a g t g t c g a c c g a c a c t a a t g a t c c t g a c c c

SbfI 3240 a g a g a t c a c g g c t t g a t t g g c t t t c a a c t g c t c t t g g t t t g c t t c a g t t c c t g c a g g a c a t c t c t a g t g c c g a a c t a a c c g a a a g t t g a c g a g a a c c a a a c g a a g t c a a g g a c g t c c t g t

3300 a t t a a t c c a c a g g t t c g a g t a a a t g t t g g a t c t t a a t g g a t a c c a g a c a c a t c a g g c t c a t a a t t a g g t g t c c a a g c t c a t t t a c a a c c t a g a a t t a c c t a t g g t c t g t g t a g t c c g a g t

3360 a c c a g a c c a c c a a g g c t t t g c g g c c c c t t t c c t c a c a t t t a a t t g t a c a a a t g a a c t t a t t g g t c t g g t g g t t c c g a a a c g c c g g g g a a a g g a g t g t a a a t t a a c a t g t t t a c t t g a a t a

3420 t c c t c c t c a c c c a a t g g c c a g a a t a a c a c t g c t t c g c g g g t t t t c t t c a t g a c g t t c a t t a g g a g g a g t g g g t t a c c g g t c t t a t t g t g a c g a a g c g c c c a a a a g a a g t a c t g c a a g t a a

3480 c t g t c c t t a c t t t g t c c t t a c t t c t g t t c c a t c t g a g t g t c g t c c t c t t c c g g g t a g t c t g a c a g g a a t g a a a c a g g a a t g a a g a c a a g g t a g a c t c a c a g c a g g a g a a g g c c c a t c a g a

3540 g c c g g c c t c c t t g a t c g t g t c t t g a g a g g t c g g a a g t t a a t c g t t t g c a c t c a g g g g g a t c g g c c g g a g g a a c t a g c a c a g a a c t c t c c a g c c t t c a a t t a g c a a a c g t g a g t c c c c c t a

PaeR7IXhoI 3600 SV40 promoter g t g t t g g a t g a a g g a c a a c a c a t c c t g c a c t c c t t g t g c t t c t c g a g a t c t g c g a t c t g c c a c a a c c t a c t t c c t g t t g t g t a g g a c g t g a g g a a c a c g a a g a g c t c t a g a c g c t a g a c g

3660 SV40 promoter SV40 ori a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g

3720 SV40 promoter SV40 ori c g c c c a g t t c c g c c c a t t c t c c g c c c c a t c g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a g c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c

StuIAvrIISfiI 3780 SV40 promoter SV40 ori c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g

HindIII 3840 1 MEDA SV40 promoter luciferase Kozak sequence MEDA t a g g c t t t t g c a a a a a g c t t g g c a t t c c g g t a c t g t t g g t a a a g c c a c c a t g g a a g a c g c a t c c g a a a a c g t t t t t c g a a c c g t a a g g c c a t g a c a a c c a t t t c g g t g g t a c c t t c t g c g

KasINarISfoIPluTI 3900 5 10 15 20 KNIKKGPAPFYPLEDGTAGE luciferase C C T C T LucNrev KNIKKGPAPFYPLEDGTAGE c a a a a a c a t a a a g a a a g g c c c g g c g c c a t t c t a t c c g c t g g a a g a t g g a a c c g c t g g a g a g t t t t t g t a t t t c t t t c c g g g c c g c g g t a a g a t a g g c g a c c t t c t a c c t t g g c g a c c t c t

3960 25 30 35 40 QLHKAMKRYALVPGTIAFTD luciferase C G T T G A C G T A T T C C LucNrev QLHKAMKRYALVPGTIAFTD g c a a c t g c a t a a g g c t a t g a a g a g a t a c g c c c t g g t t c c t g g a a c a a t t g c t t t t a c a g a c g t t g a c g t a t t c c g a t a c t t c t c t a t g c g g g a c c a a g g a c c t t g t t a a c g a a a a t g t c t

BstBI 4020 45 50 55 60 AHIEVDITYAEYFEMSVRLA luciferase AHIEVDITYAEYFEMSVRLA t g c a c a t a t c g a g g t g g a c a t c a c t t a c g c t g a g t a c t t c g a a a t g t c c g t t c g g t t g g c a c g t g t a t a g c t c c a c c t g t a g t g a a t g c g a c t c a t g a a g c t t t a c a g g c a a g c c a a c c g

4080 65 70 75 80 EAMKRYGLNTNHRIVVCSEN luciferase EAMKRYGLNTNHRIVVCSEN a g a a g c t a t g a a a c g a t a t g g g c t g a a t a c a a a t c a c a g a a t c g t c g t a t g c a g t g a a a a t c t t c g a t a c t t t g c t a t a c c c g a c t t a t g t t t a g t g t c t t a g c a g c a t a c g t c a c t t t t

4140 85 90 95 100 SLQFFMPVLGALFIGVAVAP luciferase SLQFFMPVLGALFIGVAVAP c t c t c t t c a a t t c t t t a t g c c g g t g t t g g g c g c g t t a t t t a t c g g a g t t g c a g t t g c g c c g a g a g a a g t t a a g a a a t a c g g c c a c a a c c c g c g c a a t a a a t a g c c t c a a c g t c a a c g c g g

4200 105 110 115 120 ANDIYNERELLNSMGISQPT luciferase ANDIYNERELLNSMGISQPT c g c g a a c g a c a t t t a t a a t g a a c g t g a a t t g c t c a a c a g t a t g g g c a t t t c g c a g c c t a c g c g c t t g c t g t a a a t a t t a c t t g c a c t t a a c g a g t t g t c a t a c c c g t a a a g c g t c g g a t g

4260 125 130 135 140 VVFVSKKGLQKILNVQKKLP luciferase VVFVSKKGLQKILNVQKKLP c g t g g t g t t c g t t t c c a a a a a g g g g t t g c a a a a a a t t t t g a a c g t g c a a a a a a a g c t c c c g c a c c a c a a g c a a a g g t t t t t c c c c a a c g t t t t t t a a a a c t t g c a c g t t t t t t t c g a g g g

4320 145 150 155 160 IIQKIIIMDSKTDYQGFQSM luciferase IIQKIIIMDSKTDYQGFQSM a a t c a t c c a a a a a a t t a t t a t c a t g g a t t c t a a a a c g g a t t a c c a g g g a t t t c a g t c g a t t t a g t a g g t t t t t t a a t a a t a g t a c c t a a g a t t t t g c c t a a t g g t c c c t a a a g t c a g c t a

4380 165 170 175 180 YTFVTSHLPPGFNEYDFVPE luciferase YTFVTSHLPPGFNEYDFVPE g t a c a c g t t c g t c a c a t c t c a t c t a c c t c c c g g t t t t a a t g a a t a c g a t t t t g t g c c a g a c a t g t g c a a g c a g t g t a g a g t a g a t g g a g g g c c a a a a t t a c t t a t g c t a a a a c a c g g t c t

4440 185 190 195 200 SFDRDKTIALIMNSSGSTGL luciferase SFDRDKTIALIMNSSGSTGL g t c c t t c g a t a g g g a c a a g a c a a t t g c a c t g a t c a t g a a c t c c t c t g g a t c t a c t g g t c t c a g g a a g c t a t c c c t g t t c t g t t a a c g t g a c t a g t a c t t g a g g a g a c c t a g a t g a c c a g a

4500 205 210 215 220 PKGVALPHRTACVRFSHARD luciferase PKGVALPHRTACVRFSHARD g c c t a a a g g t g t c g c t c t g c c t c a t a g a a c t g c c t g c g t g a g a t t c t c g c a t g c c a g a g a c g g a t t t c c a c a g c g a g a c g g a g t a t c t t g a c g g a c g c a c t c t a a g a g c g t a c g g t c t c t

4560 225 230 235 240 PIFGNQIIPDTAILSVVPFH luciferase PIFGNQIIPDTAILSVVPFH t c c t a t t t t t g g c a a t c a a a t c a t t c c g g a t a c t g c g a t t t t a a g t g t t g t t c c a t t c c a a g g a t a a a a a c c g t t a g t t t a g t a a g g c c t a t g a c g c t a a a a t t c a c a a c a a g g t a a g g t

XcmI 4620 245 250 255 260 HGFGMFTTLGYLICGFRVVL luciferase HGFGMFTTLGYLICGFRVVL t c a c g g t t t t g g a a t g t t t a c t a c a c t c g g a t a t t t g a t a t g t g g a t t t c g a g t c g t c t t a g t g c c a a a a c c t t a c a a a t g a t g t g a g c c t a t a a a c t a t a c a c c t a a a g c t c a g c a g a a

4680 265 270 275 280 MYRFEEELFLRSLQDYKIQS luciferase MYRFEEELFLRSLQDYKIQS a a t g t a t a g a t t t g a a g a a g a g c t g t t t c t g a g g a g c c t t c a g g a t t a c a a g a t t c a a a g t t a c a t a t c t a a a c t t c t t c t c g a c a a a g a c t c c t c g g a a g t c c t a a t g t t c t a a g t t t c

4740 285 290 295 300 ALLVPTLFSFFAKSTLIDKY luciferase ALLVPTLFSFFAKSTLIDKY t g c g c t g c t g g t g c c a a c c c t a t t c t c c t t c t t c g c c a a a a g c a c t c t g a t t g a c a a a t a a c g c g a c g a c c a c g g t t g g g a t a a g a g g a a g a a g c g g t t t t c g t g a g a c t a a c t g t t t a t

4800 305 310 315 320 DLSNLHEIASGGAPLSKEVG luciferase DLSNLHEIASGGAPLSKEVG c g a t t t a t c t a a t t t a c a c g a a a t t g c t t c t g g t g g c g c t c c c c t c t c t a a g g a a g t c g g g c t a a a t a g a t t a a a t g t g c t t t a a c g a a g a c c a c c g c g a g g g g a g a g a t t c c t t c a g c c

4860 325 330 335 340 EAVAKRFHLPGIRQGYGLTE luciferase EAVAKRFHLPGIRQGYGLTE g g a a g c g g t t g c c a a g a g g t t c c a t c t g c c a g g t a t c a g g c a a g g a t a t g g g c t c a c t g a c c t t c g c c a a c g g t t c t c c a a g g t a g a c g g t c c a t a g t c c g t t c c t a t a c c c g a g t g a c t

4920 345 350 355 360 TTSAILITPEGDDKPGAVGK luciferase TTSAILITPEGDDKPGAVGK g a c t a c a t c a g c t a t t c t g a t t a c a c c c g a g g g g g a t g a t a a a c c g g g c g c g g t c g g t a a c t g a t g t a g t c g a t a a g a c t a a t g t g g g c t c c c c c t a c t a t t t g g c c c g c g c c a g c c a t t

4980 365 370 375 380 VVPFFEAKVVDLDTGKTLGV luciferase VVPFFEAKVVDLDTGKTLGV a g t t g t t c c a t t t t t t g a a g c g a a g g t t g t g g a t c t g g a t a c c g g g a a a a c g c t g g g c g t t c a a c a a g g t a a a a a a c t t c g c t t c c a a c a c c t a g a c c t a t g g c c c t t t t g c g a c c c g c a

5040 385 390 395 400 NQRGELCVRGPMIMSGYVNN luciferase NQRGELCVRGPMIMSGYVNN t a a t c a a a g a g g c g a a c t g t g t g t g a g a g g t c c t a t g a t t a t g t c c g g t t a t g t a a a c a a a t t a g t t t c t c c g c t t g a c a c a c a c t c t c c a g g a t a c t a a t a c a g g c c a a t a c a t t t g t t

5100 405 410 415 420 PEATNALIDKDGWLHSGDIA luciferase PEATNALIDKDGWLHSGDIA t c c g g a a g c g a c c a a c g c c t t g a t t g a c a a g g a t g g a t g g c t a c a t t c t g g a g a c a t a g c a g g c c t t c g c t g g t t g c g g a a c t a a c t g t t c c t a c c t a c c g a t g t a a g a c c t c t g t a t c g

5160 425 430 435 440 YWDEDEHFFIVDRLKSLIKY luciferase YWDEDEHFFIVDRLKSLIKY t t a c t g g g a c g a a g a c g a a c a c t t c t t c a t c g t t g a c c g c c t g a a g t c t c t g a t t a a g t a a a t g a c c c t g c t t c t g c t t g t g a a g a a g t a g c a a c t g g c g g a c t t c a g a g a c t a a t t c a t

5220 445 450 455 460 KGYQVAPAELESILLQHPNI luciferase KGYQVAPAELESILLQHPNI c a a a g g c t a t c a g g t g g c t c c c g c t g a a t t g g a a t c c a t c t t g c t c c a a c a c c c c a a c a t g t t t c c g a t a g t c c a c c g a g g g c g a c t t a a c c t t a g g t a g a a c g a g g t t g t g g g g t t g t a

SgrAI 5280 465 470 475 480 FDAGVAGLPDDDAGELPAAV luciferase FDAGVAGLPDDDAGELPAAV c t t c g a c g c a g g t g t c g c a g g t c t t c c c g a c g a t g a c g c c g g t g a a c t t c c c g c c g c c g t g a a g c t g c g t c c a c a g c g t c c a g a a g g g c t g c t a c t g c g g c c a c t t g a a g g g c g g c g g c a

5340 485 490 495 500 VVLEHGKTMTEKEIVDYVAS luciferase VVLEHGKTMTEKEIVDYVAS t g t t g t t t t g g a g c a c g g a a a g a c g a t g a c g g a a a a a g a g a t c g t g g a t t a c g t c g c c a g a c a a c a a a a c c t c g t g c c t t t c t g c t a c t g c c t t t t t c t c t a g c a c c t a a t g c a g c g g t c

5400 505 510 515 520 QVTTAKKLRGGVVFVDEVPK luciferase QVTTAKKLRGGVVFVDEVPK t c a a g t a a c a a c c g c g a a a a a g t t g c g c g g a g g a g t t g t g t t t g t g g a c g a a g t a c c g a a a g t t c a t t g t t g g c g c t t t t t c a a c g c g c c t c c t c a a c a c a a a c a c c t g c t t c a t g g c t t

5460 525 530 535 540 GLTGKLDARKIREILIKAKK luciferase GLTGKLDARKIREILIKAKK a g g t c t t a c c g g a a a a c t c g a c g c a a g a a a a a t c a g a g a g a t c c t c a t a a a g g c c a a g a a t c c a g a a t g g c c t t t t g a g c t g c g t t c t t t t t a g t c t c t c t a g g a g t a t t t c c g g t t c t t

FseIXbaI 5520 545 550 GGKIAV* luciferase GGKIAV* g g g c g g a a a g a t c g c c g t g t a a t t c t a g a g t c g g g g c g g c c g g c c g c t t c g a g c a g a c a t c c c g c c t t t c t a g c g g c a c a t t a a g a t c t c a g c c c c g c c g g c c g g c g a a g c t c g t c t g t a

5580 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a

5640 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t

HpaI 5700 SV40 poly(A) signal a g t t a a c a a c a a c a a t t g c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t c a a t t g t t g t t g t t a a c g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a

BamHI 5760 t t t t t a a a g c a a g t a a a a c c t c t a c a a a t g t g g t a a a a t c g a t a a g g a t c t g g a t c c g t c a a a a a t t t c g t t c a t t t t g g a g a t g t t t a c a c c a t t t t a g c t a t t c c t a g a c c t a g g c a g

5820 g a c c g a t g c c c t t g a g a g c c t t c a a c c c a g t c a g c t c c t t c c g g t g g g c g c g g g g c a t g a c t g g c t a c g g g a a c t c t c g g a a g t t g g g t c a g t c g a g g a a g g c c a c c c g c g c c c c g t a c t

PshAI 5880 c t a t c g t c g c c g c a c t t a t g a c t g t c t t c t t t a t c a t g c a a c t c g t a g g a c a g g t g c c g g g a t a g c a g c g g c g t g a a t a c t g a c a g a a g a a a t a g t a c g t t g a g c a t c c t g t c c a c g g c c

AfeI 5940 G A G C G A G T G A C T G A G C G A L4440 c a g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g t c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t

6000 g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t

6060 g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c

6120 ori c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a

6180 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g

6240 ori c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a

6300 ori t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g

6360 ori g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t

6420 ori t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t

6480 ori g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c

6540 ori t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c

6600 ori c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a

6660 ori a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t

6720 g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c

6780 a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t

6840 285 *WHK AmpR *WHK a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t

6900 280 275 270 265 ILSAGIEAIQRNREDMTAQS AmpR ILSAGIEAIQRNREDMTAQS a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g

6960 260 255 250 245 GTTYIVVIRSPKGDPGLAAI AmpR GTTYIVVIRSPKGDPGLAAI c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c

7020 240 235 230 225 IGRSGREGAGSKDAIFWGAP AmpR IGRSGREGAGSKDAIFWGAP a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t

7080 220 215 210 205 LASRLLPGAVKDAEMWDILQ AmpR LASRLLPGAVKDAEMWDILQ a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a

7140 200 195 190 185 QRSALTLLEGTLLKRLTTAM AmpR QRSALTLLEGTLLKRLTTAM t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a

7200 180 175 170 165 AVPMTTDREDNPIAENLEPE AmpR AVPMTTDREDNPIAENLEPE g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g

7260 160 155 150 145 WRDLRTVHDGMNHLFATLEK AmpR WRDLRTVHDGMNHLFATLEK c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g

7320 140 135 130 125 PGGITTLLLNAATNDSMTIA AmpR PGGITTLLLNAATNDSMTIA g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t

7380 120 115 110 105 ASCLERVTMGDTLHKETVPS AmpR ASCLERVTMGDTLHKETVPS g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c

7440 100 95 90 85 YEVLDNQSYHIRRGLQEQGA AmpR YEVLDNQSYHIRRGLQEQGA t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c

7500 80 75 70 65 DIRSLVAGCLLVKFTSMMPF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R DIRSLVAGCLLVKFTSMMPF t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t

7560 60 55 50 45 REEPRFSELIKGSNLDLEIY AmpR REEPRFSELIKGSNLDLEIY c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t

7620 40 35 30 25 GVRAGLQDEADKVKVLTEPH AmpR GVRAGLQDEADKVKVLTEPH c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t

7680 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFHQ AmpR AFVPLCFAAFFPILAVRFHQ g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t

7740 1 signal sequence ISM AmpR AmpR promoter ISM a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c

7800 AmpR promoter a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a

7860 f1 ori c c c c g a a a a g t g c c a c c t g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g g g g c t t t t c a c g g t g g a c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c

7920 f1 ori G A G G A A A G C G A A A G F1ori-R g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g

7980 f1 ori A A G G G A F1ori-R t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g

8040 f1 ori c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a

BsaAIDraIII 8100 f1 ori g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c

8160 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c

8220 f1 ori g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c

8280 f1 ori c t g a t t t a a c a a a a a t t t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t g c c a t g a c t a a a t t g t t t t t a a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a c g g t a

8340 t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t

NotI 8400 c g c c a g c c c a a g c t a c c a t g a t a a g t a a g t a a t a t t a a g g t a c g g g a g g t a c t t g g a g c g g c g g t c g g g t t c g a t g g t a c t a t t c a t t c a t t a t a a t t c c a t g c c c t c c a t g a a c c t c g c

8460 poly(A) signal g c c g c a a t a a a a t a t c t t t a t t t t c a t t a c a t c t g t g t g t t g g t t t t t t g t g t g a a t c g a c g g c g t t a t t t t a t a g a a a t a a a a g t a a t g t a g a c a c a c a a c c a a a a a a c a c a c t t a g c t

8520 pause site C T A G C A A A A T A G G RVprimer3 t a g t a c t a a c a t a c g c t c t c c a t c a a a a c a a a a c g a a a c a a a a c a a a c t a g c a a a a t a g g a t c a t g a t t g t a t g c g a g a g g t a g t t t t g t t t t g c t t t g t t t t g t t t g a t c g t t t t a t c c

3ʹ 5ʹ 8566 pause site C T G T C C C RVprimer3 c t g t c c c c a g t g c a a g t g c a g g t g c c a g a a c a t t t c t c t a t c g a t a g a c a g g g g t c a c g t t c a c g t c c a c g g t c t t g t a a a g a g a t a g c t a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.