Addgene GFP-Rab7Q67L Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: GFP-Rab7Q67L Sequencing Result


Map View

EagI (5337) Neo-R (5303 .. 5322) PluTI (5248) SfoI (5246) NarI (5245) KasI (5244) FspI (5145) PflFI - Tth111I (5128) BsrDI (5016) RsrII (4728) Neo-F (4693 .. 4712) TK-pA-R (4375 .. 4394) EcoO109I (4146) ApaLI (3639) pBR322ori-F (3466 .. 3485) PciI (3325) AseI (3262) NdeI (3035) SnaBI (2931) CMV-F (2733 .. 2753) BmtI (2680) NheI (2676) BspDI * - ClaI * (5405) SfiI (57) CsiI - SexAI * (235) pBABE 3' (307 .. 327) F1ori-R (570 .. 589) DraIII (716) F1ori-F (780 .. 801) MluI (939) EBV-rev (956 .. 975) SV40pA-R (1010 .. 1029) HpaI (1066) MfeI (1075) BclI * (1168) XbaI * (1178) BbvCI (1195) AflII (1295) BstXI (1340) XcmI (1673) BbsI (1748) HA HA TspMI - XmaI (1881) SmaI (1883) PspOMI (1884) ApaI (1888) SacII (1891) SalI (1898) AccI (1899) EcoRI (1908) HindIII (1915) Eco53kI (1923) PaeR7I - XhoI (1924) SacI (1925) BglII (1928) EGFP-C (1985 .. 2006) EXFP-R (2334 .. 2353) EGFP-N (2593 .. 2614) AgeI (2667) AfeI (2675) GFP-Rab7Q67L 5418 bp

Sequence View

SfiI 5ʹ 3ʹ 60 SV40 promoter SV40 ori a g c c t a g g c c t c c a a a a a a g c c t c c t c a c t a c t t c t g g a a t a g c t c a g a g g c c g a g g c g g t c g g a t c c g g a g g t t t t t t c g g a g g a g t g a t g a a g a c c t t a t c g a g t c t c c g g c t c c g c c

120 SV40 promoter SV40 ori c c t c g g c c t c t g c a t a a a t a a a a a a a a t t a g t c a g c c a t g g g g c g g a g a a t g g g c g g a a c g g a g c c g g a g a c g t a t t t a t t t t t t t t a a t c a g t c g g t a c c c c g c c t c t t a c c c g c c t t g

180 SV40 promoter SV40 ori SV40 enhancer t g g g c g g a g t t a g g g g c g g g a t g g g c g g a g t t a g g g g c g g g a c t a t g g t t g c t g a c t a a t a c c c g c c t c a a t c c c c g c c c t a c c c g c c t c a a t c c c c g c c c t g a t a c c a a c g a c t g a t t a

SexAI*CsiI 240 SV40 promoter SV40 enhancer t g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t t t c c a c a c c t g g a c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a a a g g t g t g g a c c

300 SV40 promoter SV40 enhancer t t g c t g a c t a a t t g a g a t g c a t g c t t t g c a t a c t t c t g c c t g c t g g g g a g c c t g g g g a c t a a c g a c t g a t t a a c t c t a c g t a c g a a a c g t a t g a a g a c g g a c g a c c c c t c g g a c c c c t g a

360 SV40 promoter AmpR promoter SV40 enhancer A C C C T A A C T G A C A C A C A T T C C pBABE 3' t t c c a c a c c c t a a c t g a c a c a c a t t c c a c a g c t g g t t c t t t c c g c c t c a g g a c t c t t c c t a a g g t g t g g g a t t g a c t g t g t g t a a g g t g t c g a c c a a g a a a g g c g g a g t c c t g a g a a g g a

420 AmpR promoter t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t

480 AmpR promoter a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g

540 f1 ori t g a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c a c t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g

600 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g

660 f1 ori c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a

DraIII 720 f1 ori t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c

780 f1 ori G F1ori-F g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c

840 f1 ori T G G A C T C T T G T T C C A A A C T G G F1ori-F t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a

900 f1 ori a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a

MluI 960 f1 ori SV40 poly(A) signal C T A C T EBV-rev t a a c g c g a a t t t t a a c a a a a t a t t a a c g c t t a c a a t t t a c g c g t t a a g a t a c a t t g a t g a a t t g c g c t t a a a a t t g t t t t a t a a t t g c g a a t g t t a a a t g c g c a a t t c t a t g t a a c t a c t

1020 SV40 poly(A) signal G A A A T T T G T G A SV40pA-R C A A A C C T G T T T G G T G EBV-rev g t t t g g a c a a a c c a c a a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a c a a a c c t g t t t g g t g t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t

MfeIHpaI 1080 SV40 poly(A) signal T G C T A T T G C SV40pA-R t g c t a t t g c t t t a t t t g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c a a c a a t t g a c g a t a a c g a a a t a a a c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g t t g t t a a c

1140 c a t t c a t t t t a t g t t t c a g g t t c a g g g g g a g g t g t g g g a g g t t t t t t a a a g c a a g t a a a a g t a a g t a a a a t a c a a a g t c c a a g t c c c c c t c c a c a c c c t c c a a a a a a t t t c g t t c a t t t t

BbvCIXbaI*BclI* 1200 *C *C *C c c t c t a c a a a t g t g g t a t g g c t g a t t a t g a t c a g t t a t c t a g a t c c g g t g g a t c c t c a g c g g a g a t g t t t a c a c c a t a c c g a c t a a t a c t a g t c a a t a g a t c t a g g c c a c c t a g g a g t c g

1260 (in frame with EGFP) SCSEASAKARDNKDLKIPEP (in frame with HA) SCSEASAKARDNKDLKIPEP SCSEASAKARDNKDLKIPEP a a c t g c a g c t t t c t g c c g a g g c c t t g g c c c g g t c a t t c t t g t c c a g t t t g a t a g g t t c a g t t g a c g t c g a a a g a c g g c t c c g g a a c c g g g c c a g t a a g a a c a g g t c a a a c t a t c c a a g t c

AflII 1320 (in frame with EGFP) FENYLEVETEQKLANRAITQ (in frame with HA) FENYLEVETEQKLANRAITQ FENYLEVETEQKLANRAITQ g a a a t t c g t t g t a c a g c t c c a c c t c c g t t t c c t g c t t a a g t g c a t t c c g t g c a a t c g t c t c t t t a a g c a a c a t g t c g a g g t g g a g g c a a a g g a c g a a t t c a c g t a a g g c a c g t t a g c a g a

BstXI 1380 (in frame with EGFP) FAQEVNIAEKASTEFYPINN (in frame with HA) FAQEVNIAEKASTEFYPINN FAQEVNIAEKASTEFYPINN g g a a c g c c t g c t c c a c g t t g a t g g c c t c c t t g g c a c t g g t c t c a a a g t a g g g a a t g t t g t c c t t g c g g a c g a g g t g c a a c t a c c g g a g g a a c c g t g a c c a g a g t t t c a t c c c t t a c a a c a

1440 (in frame with EGFP) KSYCWAQARKTAVQRNELDI (in frame with HA) KSYCWAQARKTAVQRNELDI KSYCWAQARKTAVQRNELDI t t t t g c t g t a g c a c c a g g c c t g t g c c c g c t t t g t g g c c a c t t g t c t g t t t t c g a g g t c a a a a a a c g a c a t c g t g g t c c g g a c a c g g g c g a a a c a c c g g t g a a c a g a c a a a a g c t c c a g t t

1500 (in frame with EGFP) KNGLVVFPFNEPDRPSAQIL (in frame with HA) KNGLVVFPFNEPDRPSAQIL KNGLVVFPFNEPDRPSAQIL t c t t g t t t c c c a a c a c a a c a a a t g g g a a g t t t t c a g g a t c t c g g g g a c t g g c c t g g a t g a a g a a c a a a g g g t t g t g t t g t t t a c c c t t c a a a a g t c c t a g a g c c c c t g a c c g g a c c t a c t

1560 (in frame with EGFP) FEDRWSDLTKFTNPATVDFV (in frame with HA) FEDRWSDLTKFTNPATVDFV FEDRWSDLTKFTNPATVDFV g a a a c t c a t c t c t c c a g c t a t c t a g g g t t t t g a a t g t g t t g g g g g c a g t c a c a t c a a a t a c t t t g a g t a g a g a g g t c g a t a g a t c c c a a a a c t t a c a c a a c c c c c g t c a g t g t a g t t t a t

1620 (in frame with EGFP) LVCCDAGRYFAVGLSQFREL (in frame with HA) LVCCDAGRYFAVGLSQFREL LVCCDAGRYFAVGLSQFREL c c a g a a c g c a g c a g t c t g c a c c t c t g t a g a a g g c c a c a c c g a g a g a c t g g a a c c g t t c c a g g t c t t g c g t c g t c a g a c g t g g a g a c a t c t t c c g g t g t g g c t c t c t g a c c t t g g c a a g g t

XcmI 1680 (in frame with EGFP) GATDWIQMTVLRDDVMVEKT (in frame with HA) GATDWIQMTVLRDDVMVEKT GATDWIQMTVLRDDVMVEKT g t c c t g c t g t g t c c c a t a t c t g c a t t g t g a c t a g c c t g t c a t c c a c c a t c a c c t c c t t g g c a g g a c g a c a c a g g g t a t a g a c g t a a c a c t g a t c g g a c a g t a g g t g g t a g t g g a g g a a c c

1740 (in frame with EGFP) LFDAGITAKYQNSFKKNVYQ (in frame with HA) LFDAGITAKYQNSFKKNVYQ LFDAGITAKYQNSFKKNVYQ t c a g a a a g t c a g c t c c t a t t g t g g c t t t g t a c t g a t t g c t g a a t t t c t t a t t c a c a t a c t a g t c t t t c a g t c g a g g a t a a c a c c g a a a c a t g a c t a a c g a c t t a a a g a a t a a g t g t a t g a

BbsI 1800 (in frame with EGFP) NMLSTKGVGSDGLIIVKLLV (in frame with HA) NMLSTKGVGSDGLIIVKLLV NMLSTKGVGSDGLIIVKLLV g g t t c a t g a g t g a t g t c t t c c c g a c t c c a g a a t c t c c c a g g a t g a t a a c c t t c a g c a a c a c c a a g t a c t c a c t a c a g a a g g g c t g a g g t c t t a g a g g g t c c t a c t a t t g g a a g t c g t t g t

1860 (in frame with EGFP) KKRSTGAYDPVDYPYAYDPV (in frame with HA) KKRSTG 5 1 AYDPVDYPY 5 AYDPV HA HA KKRSTGAYDPVDYPYAYDPV c t t t c t t c c t a g a g g t a c c a g c g t a a t c c g g a a c a t c g t a t g g g t a a g c g t a a t c c g g a a g a a a g a a g g a t c t c c a t g g t c g c a t t a g g c c t t g t a g c a t a c c c a t t c g c a t t a g g c c t t

HindIIIEcoRISalIAccITspMIXmaISmaIPspOMIApaISacII 1920 (in frame with EGFP) DYPYMSGPGATGDVASNSAQ (in frame with EGFP) MSGPGATGDVASNSAQ 1 DYPY HA MCS DYPYMSGPGATGDVASNSAQ c a t c g t a t g g g t a c a t g g a t c c c g g g c c c g c g g t a c c g t c g a c t g c a g a a t t c g a a g c t t g t a g c a t a c c c a t g t a c c t a g g g c c c g g g c g c c a t g g c a g c t g a c g t c t t a a g c t t c g a a

Eco53kIPaeR7IXhoISacIBglII 1980 (in frame with EGFP) ARSRLGS 235 230 KYLEDMGLTIGAA EGFP (in frame with EGFP) ARSRLGS 235 230 KYLEDMGLTIGAA EGFP MCS ARSRLGSKYLEDMGLTIGAA g a g c t c g a g a t c t g a g t c c g g a c t t g t a c a g c t c g t c c a t g c c g a g a g t g a t c c c g g c g g c t c g a g c t c t a g a c t c a g g c c t g a a c a t g t c g a g c a g g t a c g g c t c t c a c t a g g g c c g c c

2040 225 220 215 210 TVFELLVMHDRKENPDKSLA EGFP 225 220 215 210 TVFELLVMHDRKENPDKSLA EGFP G T G C T T G A G G T C G T C C T G G T A C EGFP-C TVFELLVMHDRKENPDKSLA c g g t c a c g a a c t c c a g c a g g a c c a t g t g a t c g c g c t t c t c g t t g g g g t c t t t g c t c a g g g g c c a g t g c t t g a g g t c g t c c t g g t a c a c t a g c g c g a a g a g c a a c c c c a g a a a c g a g t c c c

2100 205 200 195 190 SQTSLYHNDPLLVPGDGIPT EGFP 205 200 195 190 SQTSLYHNDPLLVPGDGIPT EGFP SQTSLYHNDPLLVPGDGIPT c g g a c t g g g t g c t c a g g t a g t g g t t g t c g g g c a g c a g c a c g g g g c c g t c g c c g a t g g g g g g c c t g a c c c a c g a g t c c a t c a c c a a c a g c c c g t c g t c g t g c c c c g g c a g c g g c t a c c c c c

2160 185 180 175 170 NQQYHDALQVSGDEINHRIK EGFP 185 180 175 170 NQQYHDALQVSGDEINHRIK EGFP NQQYHDALQVSGDEINHRIK t g t t c t g c t g g t a g t g g t c g g c g a g c t g c a c g c t g c c g t c c t c g a t g t t g t g g c g g a t c t a c a a g a c g a c c a t c a c c a g c c g c t c g a c g t g c g a c g g c a g g a g c t a c a a c a c c g c c t a g a

2220 165 160 155 150 FNVKIGNKQKDAMIYVNHSN EGFP 165 160 155 150 FNVKIGNKQKDAMIYVNHSN EGFP FNVKIGNKQKDAMIYVNHSN t g a a g t t c a c c t t g a t g c c g t t c t t c t g c t t g t c g g c c a t g a t a t a g a c g t t g t g g c t g t a c t t c a a g t g g a a c t a c g g c a a g a a g a c g a a c a g c c g g t a c t a t a t c t g c a a c a c c g a c a

2280 145 140 135 130 YNYELKHGLINGDEKFDIGK EGFP 145 140 135 130 YNYELKHGLINGDEKFDIGK EGFP YNYELKHGLINGDEKFDIGK t g t a g t t g t a c t c c a g c t t g t g c c c c a g g a t g t t g c c g t c c t c c t t g a a g t c g a t g c c c t a c a t c a a c a t g a g g t c g a a c a c g g g g t c c t a c a a c g g c a g g a g g a a c t t c a g c t a c g g g a

2340 125 120 115 110 LEIRNVLTDGEFKVEARTKY EGFP 125 120 115 110 LEIRNVLTDGEFKVEARTKY EGFP G T C T T G T EXFP-R LEIRNVLTDGEFKVEARTKY t c a g c t c g a t g c g g t t c a c c a g g g t g t c g c c c t c g a a c t t c a c c t c g g c g c g g g t c t t g t a g t c g a g c t a c g c c a a g t g g t c c c a c a g c g g g a g c t t g a a g t g g a g c c g c g c c c a g a a c a

2400 105 100 95 90 NGDDKFFITREQVYGEPMAS EGFP 105 100 95 90 NGDDKFFITREQVYGEPMAS EGFP A G T T G C C G T C G T C EXFP-R NGDDKFFITREQVYGEPMAS a g t t g c c g t c g t c c t t g a a g a a g a t g g t g c g c t c c t g g a c g t a g c c t t c g g g c a t g g c g g t c a a c g g c a g c a g g a a c t t c t t c t a c c a c g c g a g g a c c t g c a t c g g a a g c c c g t a c c g c c

2460 85 80 75 70 KFFDHQKMHDPYRSFCQVGY EGFP 85 80 75 70 KFFDHQKMHDPYRSFCQVGY EGFP KFFDHQKMHDPYRSFCQVGY a c t t g a a g a a g t c g t g c t g c t t c a t g t g g t c g g g g t a g c g g c t g a a g c a c t g c a c g c c g t t g a a c t t c t t c a g c a c g a c g a a g t a c a c c a g c c c c a t c g c c g a c t t c g t g a c g t g c g g c a

2520 65 60 55 50 TLTTVLTPWPVPLKGTTCIF EGFP 65 60 55 50 TLTTVLTPWPVPLKGTTCIF EGFP TLTTVLTPWPVPLKGTTCIF a g g t c a g g g t g g t c a c g a g g g t g g g c c a g g g c a c g g g c a g c t t g c c g g t g g t g c a g a t g a t c c a g t c c c a c c a g t g c t c c c a c c c g g t c c c g t g c c c g t c g a a c g g c c a c c a c g t c t a c t

2580 45 40 35 30 KLTLKGYTADGEGEGSVSFK EGFP 45 40 35 30 KLTLKGYTADGEGEGSVSFK EGFP KLTLKGYTADGEGEGSVSFK a c t t c a g g g t c a g c t t g c c g t a g g t g g c a t c g c c c t c g c c c t c g c c g g a c a c g c t g a a c t t g a a g t c c c a g t c g a a c g g c a t c c a c c g t a g c g g g a g c g g g a g c g g c c t g t g c g a c t t g a

2640 25 20 15 10 HGNVDGDLEVLIPVVGTFLE EGFP 25 20 15 10 HGNVDGDLEVLIPVVGTFLE EGFP C G T C G C C G T C C A G C T C G A C C A G EGFP-N HGNVDGDLEVLIPVVGTFLE t g t g g c c g t t t a c g t c g c c g t c c a g c t c g a c c a g g a t g g g c a c c a c c c c g g t g a a c a g c t a c a c c g g c a a a t g c a g c g g c a g g t c g a g c t g g t c c t a c c c g t g g t g g g g c c a c t t g t c g a

BmtIAfeINheIAgeI 2700 5 EGKS 1a V 1 M EGFP 5 EGKS 1a V 1 M EGFP Kozak sequence EGKSVM c c t c g c c c t t g c t c a c c a t g g t g g c g a c c g g t a g c g c t a g c g g a t c t g a c g g t t c a c t a a g g a g c g g g a a c g a g t g g t a c c a c c g c t g g c c a t c g c g a t c g c c t a g a c t g c c a a g t g a t t

2760 CMV promoter G T G C G G A T G G C G G G T A A A C G C CMV-F a c c a g c t c t g c t t a t a t a g a c c t c c c a c c g t a c a c g c c t a c c g c c c a t t t g c g t c a a t g g t g g t c g a g a c g a a t a t a t c t g g a g g g t g g c a t g t g c g g a t g g c g g g t a a a c g c a g t t a c c

2820 CMV promoter g g c g g a g t t g t t a c g a c a t t t t g g a a a g t c c c g t t g a t t t t g g t g c c a a a a c a a a c t c c c c c g c c t c a a c a a t g c t g t a a a a c c t t t c a g g g c a a c t a a a a c c a c g g t t t t g t t t g a g g g

2880 CMV promoter a t t g a c g t c a a t g g g g t g g a g a c t t g g a a a t c c c c g t g a g t c a a a c c g c t a t c c a c g c c c t a a c t g c a g t t a c c c c a c c t c t g a a c c t t t a g g g g c a c t c a g t t t g g c g a t a g g t g c g g g

SnaBI 2940 CMV promoter CMV enhancer a t t g a t g t a c t g c c a a a a c c g c a t c a c c a t g g t a a t a g c g a t g a c t a a t a c g t a g a t g t a t a a c t a c a t g a c g g t t t t g g c g t a g t g g t a c c a t t a t c g c t a c t g a t t a t g c a t c t a c a t

3000 CMV enhancer c t g c c a a g t a g g a a a g t c c c a t a a g g t c a t g t a c t g g g c a t a a t g c c a g g c g g g c c a t t t g a c g g t t c a t c c t t t c a g g g t a t t c c a g t a c a t g a c c c g t a t t a c g g t c c g c c c g g t a a a

NdeI 3060 CMV enhancer a c c g t c a t t g a c g t c a a t a g g g g g c g t a c t t g g c a t a t g a t a c a c t t g a t g t a c t g c c a a t g g c a g t a a c t g c a g t t a t c c c c c g c a t g a a c c g t a t a c t a t g t g a a c t a c a t g a c g g t t

3120 CMV enhancer g t g g g c a g t t t a c c g t a a a t a c t c c a c c c a t t g a c g t c a a t g g a a a g t c c c t a t t g g c g t c a c c c g t c a a a t g g c a t t t a t g a g g t g g g t a a c t g c a g t t a c c t t t c a g g g a t a a c c g c a

3180 CMV enhancer t a c t a t g g g a a c a t a c g t c a t t a t t g a c g t c a a t g g g c g g g g g t c g t t g g g c g g t c a g c c a t g a t a c c c t t g t a t g c a g t a a t a a c t g c a g t t a c c c g c c c c c a g c a a c c c g c c a g t c g g

3240 CMV enhancer a g g c g g g c c a t t t a c c g t a a g t t a t g t a a c g c g g a a c t c c a t a t a t g g g c t a t g a a c t a a t c c g c c c g g t a a a t g g c a t t c a a t a c a t t g c g c c t t g a g g t a t a t a c c c g a t a c t t g a t t

AseI 3300 t g a c c c c g t a a t t g a t t a c t a t t a a t a a c t a a t g c a t g g c g g t a a t a c g g t t a t c c a c a g a c t g g g g c a t t a a c t a a t g a t a a t t a t t g a t t a c g t a c c g c c a t t a t g c c a a t a g g t g t c

PciI 3360 a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g

3420 ori g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t

3480 ori T T T C T A T G G T C C G C A pBR322ori-F a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a

3540 ori A A G G G pBR322ori-F t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g

3600 ori t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g

ApaLI 3660 ori t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g

3720 ori c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a

3780 ori t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c

3840 ori c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t

3900 ori t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t

3960 ori a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t

4020 ori a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c

4080 a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g

4140 *LIYSYGSAI t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a c c t g a g g c t a a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t g g a c t c c g a t

EcoO109I 4200 APGAAGVNAALGPGEGSSPP t g g c a g g g c c t g c c g c c c c g a c g t t g g c t g c g a g c c c t g g g c c t t c a c c c g a a c t t g g g g a c c g t c c c g g a c g g c g g g g c t g c a a c c g a c g c t c g g g a c c c g g a a g t g g g c t t g a a c c c c

4260 HPPSFSSVRAYQGWHPRPPT g g t g g g g t g g g g a a a a g g a a g a a a c g c g g g c g t a t t g g c c c c a a t g g g g t c t c g g t g g g g c c a c c c c a c c c c t t t t c c t t c t t t g c g c c c g c a t a a c c g g g g t t a c c c c a g a g c c a c c c c

4320 HSV TK poly(A) signal DVSHWGQSRVGRKHVFSGVG t a t c g a c a g a g t g c c a g c c c t g g g a c c g a a c c c c g c g t t t a t g a a c a a a c g a c c c a a c a c a t a g c t g t c t c a c g g t c g g g a c c c t g g c t t g g g g c g c a a a t a c t t g t t t g c t g g g t t g t g

4380 HSV TK poly(A) signal T T G T C T TK-pA-R HTKNQRKIATMARTGEPITE c g t g c g t t t t a t t c t g t c t t t t t a t t g c c g t c a t a g c g c g g g t t c c t t c c g g t a t t g t c t g c a c g c a a a a t a a g a c a g a a a a a t a a c g g c a g t a t c g c g c c c a a g g a a g g c c a t a a c a g a

4440 C C T T C C G T G T T T C A TK-pA-R KRTETLRGRPHAFFELMLDG c c t t c c g t g t t t c a g t t a g c c t c c c c c t a g g g t g g g c g a a g a a c t c c a g c a t g a g a t c c c g g a a g g c a c a a a g t c a a t c g g a g g g g g a t c c c a c c c g c t t c t t g a g g t c g t a c t c t a g g g

4500 RQLIMWGADRFVIGFGLREY c g c g c t g g a g g a t c a t c c a g c c g g c g t c c c g g a a a a c g a t t c c g a a g c c c a a c c t t t c a t g c g c g a c c t c c t a g t a g g t c g g c c g c a g g g c c t t t t g c t a a g g c t t c g g g t t g g a a a g t a

4560 MAGWASLGRS FAATSDFDRSPLNPTAQDTM a g a a g g c g g c g g t g g a a t c g a a a t c t c g t g a t g g c a g g t t g g g c g t c g c t t g g t c g g t c a t c t t c c g c c g c c a c c t t a g c t t t a g a g c a c t a c c g t c c a a c c c g c a g c g a a c c a g c c a g t

4620 265 260 255 *FFEDLLRYFAIR NeoR/KanR 265 260 255 *FFEDLLRYFAIR NeoR/KanR FRTPESRSEELVKKAIEGDA *FFEDLLRYFAIR t t t c g a a c c c c a g a g t c c c g c t c a g a a g a a c t c g t c a a g a a g g c g a t a g a a g g c g a t g c g a a a g c t t g g g g t c t c a g g g c g a g t c t t c t t g a g c a g t t c t t c c g c t a t c t t c c g c t a c g c

4680 250 245 240 235 QSDPAAIGYLVLFRDAWEGG NeoR/KanR 250 245 240 235 QSDPAAIGYLVLFRDAWEGG NeoR/KanR LRIGSGDTVKHEEAVSPFAA QSDPAAIGYLVLFRDAWEGG c t g c g a a t c g g g a g c g g c g a t a c c g t a a a g c a c g a g g a a g c g g t c a g c c c a t t c g c c g c c g a c g c t t a g c c c t c g c c g c t a t g g c a t t t c g t g c t c c t t c g c c a g t c g g g t a a g c g g c g g

RsrII 4740 230 225 220 215 LEEAIDRTALAIDQYRDAVG NeoR/KanR 230 225 220 215 LEEAIDRTALAIDQYRDAVG NeoR/KanR T T A T A G T G C C C A T C G G T T G C Neo-F KLFSNITGSQRYVLIAVRHT LEEAIDRTALAIDQYRDAVG a a g c t c t t c a g c a a t a t c a c g g g t a g c c a a c g c t a t g t c c t g a t a g c g g t c c g c c a c a c c t t c g a g a a g t c g t t a t a g t g c c c a t c g g t t g c g a t a c a g g a c t a t c g c c a g g c g g t g t g g

4800 210 205 200 195 LRGCDIFGSFRGNEVMINPL NeoR/KanR 210 205 200 195 LRGCDIFGSFRGNEVMINPL NeoR/KanR QPATVDESRKAAIFHHDIRQ LRGCDIFGSFRGNEVMINPL c a g c c g g c c a c a g t c g a t g a a t c c a g a a a a g c g g c c a t t t t c c a c c a t g a t a t t c g g c a a g t c g g c c g g t g t c a g c t a c t t a g g t c t t t t c g c c g g t a a a a g g t g g t a c t a t a a g c c g t t

4860 190 185 180 175 CADGHTVVLDEGDPMSAKLR NeoR/KanR 190 185 180 175 CADGHTVVLDEGDPMSAKLR NeoR/KanR AGIAMGHDEILAVGHARLEP CADGHTVVLDEGDPMSAKLR g c a g g c a t c g c c a t g g g t c a c g a c g a g a t c c t c g c c g t c g g g c a t g c t c g c c t t g a g c c t c g t c c g t a g c g g t a c c c a g t g c t g c t c t a g g a g c g g c a g c c c g t a c g a g c g g a a c t c g g a

4920 170 165 160 155 AFLEAPALGQHEEDLDDQDV NeoR/KanR 170 165 160 155 AFLEAPALGQHEEDLDDQDV NeoR/KanR GEQFGWREPLMLFVQIILID AFLEAPALGQHEEDLDDQDV g g c g a a c a g t t c g g c t g g c g c g a g c c c c t g a t g c t c t t c g t c c a g a t c a t c c t g a t c g a c c c g c t t g t c a a g c c g a c c g c g c t c g g g g a c t a c g a g a a g c a g g t c t a g t a g g a c t a g c t g

4980 150 145 140 135 LGAEMRTRAREIRHKAQHDF NeoR/KanR 150 145 140 135 LGAEMRTRAREIRHKAQHDF NeoR/KanR KTGFHPSTCSLDAMFRLVVE LGAEMRTRAREIRHKAQHDF a a g a c c g g c t t c c a t c c g a g t a c g t g c t c g c t c g a t g c g a t g t t t c g c t t g g t g g t c g a a t t c t g g c c g a a g g t a g g c t c a t g c a c g a g c g a g c t a c g c t a c a a a g c g a a c c a c c a g c t t

BsrDI 5040 130 125 120 115 PCTAPDLTHLRRMADAMISV NeoR/KanR 130 125 120 115 PCTAPDLTHLRRMADAMISV NeoR/KanR WAGSRIKRMQPPHCISHDGY PCTAPDLTHLRRMADAMISV t g g g c a g g t a g c c g g a t c a a g c g t a t g c a g c c g c c g c a t t g c a t c a g c c a t g a t g g a t a c a c c c g t c c a t c g g c c t a g t t c g c a t a c g t c g g c g g c g t a a c g t a g t c g g t a c t a c c t a t g

5100 110 105 100 95 KEAPALHSSLLDQGPVEGLL NeoR/KanR 110 105 100 95 KEAPALHSSLLDQGPVEGLL NeoR/KanR FLGRSKVR* KEAPALHSSLLDQGPVEGLL t t t c t c g g c a g g a g c a a g g t g a g a t g a c a g g a g a t c c t g c c c c g g c a c t t c g c c c a a t a g a a a g a g c c g t c c t c g t t c c a c t c t a c t g t c c t c t a g g a c g g g g c c g t g a a g c g g g t t a t c

FspITth111IPflFI 5160 90 85 80 75 LWDRGAETVVDLVAACPVGT NeoR/KanR 90 85 80 75 LWDRGAETVVDLVAACPVGT NeoR/KanR LWDRGAETVVDLVAACPVGT c a g c c a g t c c c t t c c c g c t t c a g t g a c a a c g t c g a g c a c a g c t g c g c a a g g a a c g c c c g t g t c g g t c a g g g a a g g g c g a a g t c a c t g t t g c a g c t c g t g t c g a c g c g t t c c t t g c g g g c a

5220 70 65 60 55 TALWSLRAAEDQLENLAGSL NeoR/KanR 70 65 60 55 TALWSLRAAEDQLENLAGSL NeoR/KanR TALWSLRAAEDQLENLAGSL c g t g g c c a g c c a c g a t a g c c g c g c t g c c t c g t c t t g c a g t t c a t t c a g g g c a c c g g a c a g g c a c c g g t c g g t g c t a t c g g c g c g a c g g a g c a g a a c g t c a a g t a a g t c c c g t g g c c t g t c

KasINarISfoIPluTI 5280 50 45 40 35 DTKVFLVPRGQASLRFVAAD NeoR/KanR 50 45 40 35 DTKVFLVPRGQASLRFVAAD NeoR/KanR DTKVFLVPRGQASLRFVAAD g t c g g t c t t g a c a a a a a g a a c c g g g c g c c c c t g c g c t g a c a g c c g g a a c a c g g c g g c a t c c a g c c a g a a c t g t t t t t c t t g g c c c g c g g g g a c g c g a c t g t c g g c c t t g t g c c g c c g t a g

EagI 5340 30 25 20 15 SCGITQQAWDYGFLREVWAA NeoR/KanR 30 25 20 15 SCGITQQAWDYGFLREVWAA NeoR/KanR G C C C A G T C A T A G C C G A A T A G Neo-R SCGITQQAWDYGFLREVWAA a g a g c a g c c g a t t g t c t g t t g t g c c c a g t c a t a g c c g a a t a g c c t c t c c a c c c a a g c g g c t c t c g t c g g c t a a c a g a c a a c a c g g g t c a g t a t c g g c t t a t c g g a g a g g t g g g t t c g c c g

5400 10 5 1 PSGAHLGDQEIM NeoR/KanR 10 5 1 PSGAHLGDQEIM NeoR/KanR PSGAHLGDQEIM c g g a g a a c c t g c g t g c a a t c c a t c t t g t t c a a t c a t g c g a a a c g a t c c t c a t c c t g t c t c g c c t c t t g g a c g c a c g t t a g g t a g a a c a a g t t a g t a c g c t t t g c t a g g a g t a g g a c a g a g

BspDI*ClaI* 3ʹ 5ʹ 5418 SV40 promoter t t g a t c g a t c t t t g c a a a a a c t a g c t a g a a a c g t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.