Addgene mKO2-Calnexin-N-14 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: mKO2-Calnexin-N-14 Sequencing Result

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AseI (6380) PciI (6315) pBR322ori-F (6160 .. 6179) ApaLI (6001) TK-pA-R (5251 .. 5270) Neo-F (4933 .. 4952) RsrII (4913) BsrDI (4630) MscI (4479) PluTI (4400) SfoI (4398) NarI (4397) KasI (4396) Neo-R (4323 .. 4342) BspDI * - ClaI * (4237) SfiI (4172) CsiI - SexAI * (3986) pBABE 3' (3900 .. 3920) F1ori-R (3638 .. 3657) DraIII (3513) F1ori-F (3426 .. 3447) AflII (3279) EBV-rev (3252 .. 3271) SV40pA-R (3198 .. 3217) NdeI (174) CMV-F (459 .. 479) NheI (531) BmtI (535) AfeI (536) PaeR7I - XhoI (553) HindIII (901) BsgI (1147) PflMI (1329) PpuMI (1395) AgeI (2370) AleI (2383) MluI (2511) AhdI (2559) FspAI (2705) NotI (3041) XbaI * (3051) mKO2-Calnexin-N-14 6433 bp

Sequence View

5ʹ 3ʹ 60 CMV enhancer c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c c a t t g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g g t a a

120 CMV enhancer g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t c a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g c a g t

NdeI 180 CMV enhancer a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a t g c c t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t a c g g

240 CMV enhancer a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a g t a t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g t c a t

300 CMV enhancer c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t t a c g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t a a t g

360 CMV enhancer CMV promoter c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c g g g g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g c c c c

420 CMV promoter a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c a a c g t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g t t g c

480 CMV promoter C G C A A A T G G G C G G T A G G C G T G CMV-F g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t a g g c g t g t c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c a t c c g c a c a

NheIBmtIAfeI 540 CMV promoter a c g g t g g g a g g t c t a t a t a a g c a g a g c t g g t t t a g t g a a c c g t c a g a t c c g c t a g c g c t a t g c c a c c c t c c a g a t a t a t t c g t c t c g a c c a a a t c a c t t g g c a g t c t a g g c g a t c g c g a t

PaeR7IXhoI 600 MEGKWLLCMLLV c c g g a c t c a g a t c t c g a g a c c g c c a t g g a a g g g a a g t g g t t g c t g t g t a t g t t a c t g g t g g g c c t g a g t c t a g a g c t c t g g c g g t a c c t t c c c t t c a c c a a c g a c a c a t a c a a t g a c c a c

660 LGTAIVEAHDGHDDDVIDIE c t t g g a a c t g c t a t t g t t g a g g c t c a t g a t g g a c a t g a t g a t g a t g t g a t t g a t a t t g a g g a a c c t t g a c g a t a a c a a c t c c g a g t a c t a c c t g t a c t a c t a c t a c a c t a a c t a t a a c t c

720 DDLDDVIEEVEDSKPDTTAP g a t g a c c t t g a c g a t g t c a t t g a a g a g g t a g a a g a c t c a a a a c c a g a t a c c a c t g c t c c t c t a c t g g a a c t g c t a c a g t a a c t t c t c c a t c t t c t g a g t t t t g g t c t a t g g t g a c g a g g a

780 PSSPKVTYKAPVPTGEVYFA c c t t c a t c t c c c a a g g t t a c t t a c a a a g c t c c a g t t c c a a c a g g g g a a g t a t a t t t t g c t g g a a g t a g a g g g t t c c a a t g a a t g t t t c g a g g t c a a g g t t g t c c c c t t c a t a t a a a a c g a

840 DSFDRGTLSGWILSKAKKDD g a t t c t t t t g a c a g a g g a a c t c t g t c a g g g t g g a t t t t a t c c a a a g c c a a g a a a g a c g a t c t a a g a a a a c t g t c t c c t t g a g a c a g t c c c a c c t a a a a t a g g t t t c g g t t c t t t c t g c t a

900 TDDEIAKYDGKWEVEEMKES a c c g a t g a t g a a a t t g c c a a a t a t g a t g g a a a g t g g g a g g t a g a g g a a a t g a a g g a g t c a t g g c t a c t a c t t t a a c g g t t t a t a c t a c c t t t c a c c c t c c a t c t c c t t t a c t t c c t c a g t

HindIII 960 KLPGDKGLVLMSRAKHHAIS a a g c t t c c a g g t g a t a a a g g a c t t g t g t t g a t g t c t c g g g c c a a g c a t c a t g c c a t c t c t t t c g a a g g t c c a c t a t t t c c t g a a c a c a a c t a c a g a g c c c g g t t c g t a g t a c g g t a g a g a

1020 AKLNKPFLFDTKPLIVQYEV g c t a a a c t g a a c a a g c c c t t c c t g t t t g a c a c c a a g c c t c t c a t t g t t c a g t a t g a g g t t c g a t t t g a c t t g t t c g g g a a g g a c a a a c t g t g g t t c g g a g a g t a a c a a g t c a t a c t c c a a

1080 NFQNGIECGGAYVKLLSKTP a a t t t c c a a a a t g g a a t a g a a t g t g g t g g t g c c t a t g t g a a a c t g c t t t c t a a a a c a c c a t t a a a g g t t t t a c c t t a t c t t a c a c c a c c a c g g a t a c a c t t t g a c g a a a g a t t t t g t g g t

1140 ELNLDQFHDKTPYTIMFGPD g a a c t c a a c c t g g a t c a g t t c c a t g a c a a g a c c c c t t a t a c g a t t a t g t t t g g t c c a g a t c t t g a g t t g g a c c t a g t c a a g g t a c t g t t c t g g g g a a t a t g c t a a t a c a a a c c a g g t c t a

BsgI 1200 KCGEDYKLHFIFRHKNPKTG a a a t g t g g a g a g g a c t a t a a a c t g c a c t t c a t c t t c c g a c a c a a a a a c c c c a a a a c g g g t t t t a c a c c t c t c c t g a t a t t t g a c g t g a a g t a g a a g g c t g t g t t t t t g g g g t t t t g c c c a

1260 IYEEKHAKRPDADLKTYFTD a t c t a t g a a g a a a a a c a t g c t a a g a g g c c a g a t g c a g a t c t g a a g a c c t a t t t t a c t g a t t a g a t a c t t c t t t t t g t a c g a t t c t c c g g t c t a c g t c t a g a c t t c t g g a t a a a a t g a c t a

1320 KKTHLYTLILNPDNSFEILV a a g a a a a c a c a t c t t t a c a c a c t a a t c t t g a a t c c a g a t a a t a g t t t t g a a a t a c t g g t t t t c t t t t g t g t a g a a a t g t g t g a t t a g a a c t t a g g t c t a t t a t c a a a a c t t t a t g a c c a a

PflMI 1380 DQSVVNSGNLLNDMTPPVNP g a c c a a t c t g t g g t g a a t a g t g g a a a t c t g c t c a a t g a c a t g a c t c c t c c t g t a a a t c c t c t g g t t a g a c a c c a c t t a t c a c c t t t a g a c g a g t t a c t g t a c t g a g g a g g a c a t t t a g g a

PpuMI 1440 SREIEDPEDRKPEDWDERPK t c a c g t g a a a t t g a g g a c c c a g a a g a c c g g a a g c c c g a g g a t t g g g a t g a a a g a c c a a a a a g t g c a c t t t a a c t c c t g g g t c t t c t g g c c t t c g g g c t c c t a a c c c t a c t t t c t g g t t t t

1500 IPDPEAVKPDDWDEDAPAKI a t c c c a g a t c c a g a a g c t g t c a a g c c a g a t g a c t g g g a t g a a g a t g c c c c t g c t a a g a t t t a g g g t c t a g g t c t t c g a c a g t t c g g t c t a c t g a c c c t a c t t c t a c g g g g a c g a t t c t a a

1560 PDEEATKPEGWLDDEPEYVP c c a g a t g a a g a g g c c a c a a a a c c c g a a g g c t g g t t a g a t g a t g a g c c t g a g t a c g t a c c t g g t c t a c t t c t c c g g t g t t t t g g g c t t c c g a c c a a t c t a c t a c t c g g a c t c a t g c a t g g a

1620 DPDAEKPEDWDEDMDGEWEA g a t c c a g a c g c a g a g a a a c c t g a g g a t t g g g a t g a a g a c a t g g a t g g a g a a t g g g a g g c t c t a g g t c t g c g t c t c t t t g g a c t c c t a a c c c t a c t t c t g t a c c t a c c t c t t a c c c t c c g a

1680 PQIANPRCESAPGCGVWQRP c c t c a g a t t g c c a a c c c t a g a t g t g a g t c a g c t c c t g g a t g t g g t g t c t g g c a g c g a c c t g g a g t c t a a c g g t t g g g a t c t a c a c t c a g t c g a g g a c c t a c a c c a c a g a c c g t c g c t g g a

1740 VIDNPNYKGKWKPPMIDNPS g t g a t t g a c a a c c c c a a t t a t a a a g g c a a a t g g a a g c c t c c t a t g a t t g a c a a t c c c a g t c a c t a a c t g t t g g g g t t a a t a t t t c c g t t t a c c t t c g g a g g a t a c t a a c t g t t a g g g t c a

1800 YQGIWKPRKIPNPDFFEDLE t a c c a g g g a a t c t g g a a a c c c a g g a a a a t a c c a a a t c c a g a t t t c t t t g a a g a t c t g g a a a t g g t c c c t t a g a c c t t t g g g t c c t t t t a t g g t t t a g g t c t a a a g a a a c t t c t a g a c c t t

1860 PFRMTPFSAIGLELWSMTSD c c t t t c a g a a t g a c t c c t t t t a g t g c t a t t g g t t t g g a g c t g t g g t c c a t g a c c t c t g a c g g a a a g t c t t a c t g a g g a a a a t c a c g a t a a c c a a a c c t c g a c a c c a g g t a c t g g a g a c t g

1920 IFFDNFIICADRRIVDDWAN a t t t t t t t t g a c a a c t t t a t c a t t t g t g c t g a t c g a a g a a t a g t t g a t g a t t g g g c c a a t t a a a a a a a a c t g t t g a a a t a g t a a a c a c g a c t a g c t t c t t a t c a a c t a c t a a c c c g g t t a

1980 DGWGLKKAADGAAEPGVVGQ g a t g g a t g g g g c c t g a a g a a a g c t g c t g a t g g g g c t g c t g a g c c a g g c g t t g t g g g g c a g c t a c c t a c c c c g g a c t t c t t t c g a c g a c t a c c c c g a c g a c t c g g t c c g c a a c a c c c c g t c

2040 MNEAAEERPWLWVVYILTVA a t g a a c g a g g c a g c t g a a g a g c g c c c g t g g c t g t g g g t a g t c t a t a t t c t a a c t g t a g c c t a c t t g c t c c g t c g a c t t c t c g c g g g c a c c g a c a c c c a t c a g a t a t a a g a t t g a c a t c g g

2100 LPVFLVILFCCSGKKQTSGM c t t c c t g t g t t c c t g g t t a t c c t c t t c t g c t g t t c t g g a a a g a a a c a g a c c a g t g g t a t g g a a g g a c a c a a g g a c c a a t a g g a g a a g a c g a c a a g a c c t t t c t t t g t c t g g t c a c c a t a c

2160 EYKKTDAPQPDVKEEEEEKE g a g t a t a a g a a a a c t g a t g c a c c t c a a c c g g a t g t g a a g g a a g a g g a a g a a g a g a a g g a a c t c a t a t t c t t t t g a c t a c g t g g a g t t g g c c t a c a c t t c c t t c t c c t t c t t c t c t t c c t t

2220 EEKDKGDEEEEGEEKLEEKQ g a g g a a a a g g a c a a g g g a g a t g a g g a g g a g g a a g g a g a a g a g a a a c t t g a a g a g a a a c a g c t c c t t t t c c t g t t c c c t c t a c t c c t c c t c c t t c c t c t t c t c t t t g a a c t t c t c t t t g t c

2280 KSDAEEDGGTVSQEEEDRKP a a a a g t g a t g c t g a a g a a g a t g g t g g c a c t g t c a g t c a a g a g g a g g a a g a c a g a a a a c c t t t t t c a c t a c g a c t t c t t c t a c c a c c g t g a c a g t c a g t t c t c c t c c t t c t g t c t t t t g g a

2340 KAEEDEILNRSPRNRKPRRE a a a g c a g a g g a g g a t g a a a t t t t g a a c a g a t c a c c a a g a a a c a g a a a g c c a c g a a g a g a g t t t c g t c t c c t c c t a c t t t a a a a c t t g t c t a g t g g t t c t t t g t c t t t c g g t g c t t c t c t c

AleIAgeI 2400 1 5 MVSVIK mKO2 Kozak sequence GGASGGSGSGPVATMVSVIK g g g g g t g c g t c a g g c g g c a g c g g a t c t g g a c c g g t c g c c a c c a t g g t g a g t g t g a t t a a a c c c c c a c g c a g t c c g c c g t c g c c t a g a c c t g g c c a g c g g t g g t a c c a c t c a c a c t a a t t t

2460 10 15 20 25 PEMKMRYYMDGSVNGHEFTI mKO2 PEMKMRYYMDGSVNGHEFTI c c a g a g a t g a a g a t g a g g t a c t a c a t g g a c g g c t c c g t c a a t g g g c a t g a g t t c a c a a t t g g t c t c t a c t t c t a c t c c a t g a t g t a c c t g c c g a g g c a g t t a c c c g t a c t c a a g t g t t a a

MluI 2520 30 35 40 45 EGEGTGRPYEGHQEMTLRVT mKO2 EGEGTGRPYEGHQEMTLRVT g a a g g t g a a g g c a c a g g c a g a c c t t a c g a g g g a c a t c a a g a g a t g a c a c t a c g c g t c a c a c t t c c a c t t c c g t g t c c g t c t g g a a t g c t c c c t g t a g t t c t c t a c t g t g a t g c g c a g t g t

AhdI 2580 50 55 60 65 MAEGGPMPFAFDLVSHVFCY mKO2 MAEGGPMPFAFDLVSHVFCY a t g g c c g a g g g c g g g c c a a t g c c t t t c g c g t t t g a c t t a g t g t c a c a c g t g t t c t g t t a c t a c c g g c t c c c g c c c g g t t a c g g a a a g c g c a a a c t g a a t c a c a g t g t g c a c a a g a c a a t g

2640 70 75 80 85 GHRVFTKYPEEIPDYFKQAF mKO2 GHRVFTKYPEEIPDYFKQAF g g c c a c a g a g t a t t t a c t a a a t a t c c a g a a g a g a t a c c a g a c t a t t t c a a a c a a g c a t t t c c g g t g t c t c a t a a a t g a t t t a t a g g t c t t c t c t a t g g t c t g a t a a a g t t t g t t c g t a a a

2700 90 95 100 105 PEGLSWERSLEFEDGGSASV mKO2 PEGLSWERSLEFEDGGSASV c c t g a a g g c c t g t c a t g g g a a a g g t c g t t g g a g t t c g a a g a t g g t g g g t c c g c t t c a g t c g g a c t t c c g g a c a g t a c c c t t t c c a g c a a c c t c a a g c t t c t a c c a c c c a g g c g a a g t c a g

FspAI 2760 110 115 120 125 SAHISLRGNTFYHKSKFTGV mKO2 SAHISLRGNTFYHKSKFTGV a g t g c g c a t a t a a g c c t t a g a g g a a a c a c c t t c t a c c a c a a a t c c a a a t t t a c t g g g g t t t c a c g c g t a t a t t c g g a a t c t c c t t t g t g g a a g a t g g t g t t t a g g t t t a a a t g a c c c c a a

2820 130 135 140 145 NFPADGPIMQNQSVDWEPST mKO2 NFPADGPIMQNQSVDWEPST a a c t t t c c t g c c g a t g g t c c t a t c a t g c a a a a c c a a a g t g t t g a t t g g g a g c c a t c a a c c t t g a a a g g a c g g c t a c c a g g a t a g t a c g t t t t g g t t t c a c a a c t a a c c c t c g g t a g t t g g

2880 150 155 160 165 EKITASDGVLKGDVTMYLKL mKO2 EKITASDGVLKGDVTMYLKL g a g a a a a t t a c t g c c a g c g a c g g a g t t c t g a a g g g t g a t g t t a c g a t g t a c c t a a a a c t t c t c t t t t a a t g a c g g t c g c t g c c t c a a g a c t t c c c a c t a c a a t g c t a c a t g g a t t t t g a a

2940 170 175 180 185 EGGGNHKCQMKTTYKAAKEI mKO2 EGGGNHKCQMKTTYKAAKEI g a a g g a g g c g g c a a t c a c a a a t g c c a a a t g a a g a c t a c t t a c a a g g c g g c a a a a g a g a t t c t t c c t c c g c c g t t a g t g t t t a c g g t t t a c t t c t g a t g a a t g t t c c g c c g t t t t c t c t a a

3000 190 195 200 205 LEMPGDHYIGHRLVRKTEGN mKO2 LEMPGDHYIGHRLVRKTEGN c t t g a a a t g c c a g g a g a c c a t t a c a t c g g c c a t c g c c t c g t c a g g a a a a c c g a a g g c a a c g a a c t t t a c g g t c c t c t g g t a a t g t a g c c g g t a g c g g a g c a g t c c t t t t g g c t t c c g t t g

XbaI*NotI 3060 210 215 ITEQVEDAVAHS* mKO2 ITEQVEDAVAHS* a t t a c t g a g c a g g t a g a a g a t g c a g t a g c t c a t a g c t a a g c g g c c g c g a c t c t a g a t c a t t a a t g a c t c g t c c a t c t t c t a c g t c a t c g a g t a t c g a t t c g c c g g c g c t g a g a t c t a g t a

3120 a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t c c c a c a c c t c c c t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a g g g t g t g g a g g g

3180 SV40 poly(A) signal c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t

3240 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a

AflII 3300 SV40 poly(A) signal f1 ori G T G G T T T G T C C A A A C T C A T C EBV-rev g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a a g g c g t a a a t t g t a a g c g c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t t c c g c a t t t a a c a t t c g c

3360 f1 ori t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a

3420 f1 ori a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c

3480 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g

DraIII 3540 f1 ori g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a

3600 f1 ori t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c

3660 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c

3720 f1 ori g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g

3780 f1 ori AmpR promoter t t a a t g c g c c g c t a c a g g g c g c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c a a t t a c g c g g c g a t g t c c c g c g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g

3840 AmpR promoter c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a

3900 AmpR promoter SV40 promoter SV40 enhancer C pBABE 3' g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t c c t g a g g c g g a a a g a a c c a g c t g t g c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a g g a c t c c g c c t t t c t t g g t c g a c a c

3960 SV40 promoter SV40 enhancer C T T A C A C A C A G T C A A T C C C A pBABE 3' g a a t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a c t t a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t

SexAI*CsiI 4020 SV40 promoter SV40 enhancer a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g t t c g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c

4080 SV40 promoter SV40 enhancer c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g

4140 SV40 promoter SV40 ori g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a

SfiI 4200 SV40 promoter SV40 ori t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c

BspDI*ClaI* 4260 SV40 promoter SV40 ori a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a g a t c g a t c a a g a g a c a g g a t g a g g a t t c c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t c t a g c t a g t t c t c t g t c c t a c t c c t a

4320 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR 1 5 10 15 MIEQDGLHAGSPAAWVE NeoR/KanR MIEQDGLHAGSPAAWVE c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g c t t g g g t g g a g a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c g a a c c c a c c t c t

4380 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR 20 25 30 35 RLFGYDWAQQTIGCSDAAVF NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R RLFGYDWAQQTIGCSDAAVF g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g c c g c c g t g t t c c c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c g g c g g c a c a a g g

KasINarISfoIPluTI 4440 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR 40 45 50 55 RLSAQGRPVLFVKTDLSGAL NeoR/KanR RLSAQGRPVLFVKTDLSGAL g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t c c g g t g c c c t g a c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a g g c c a c g g g a c t

MscI 4500 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR 60 65 70 75 NELQDEAARLSWLATTGVPC NeoR/KanR NELQDEAARLSWLATTGVPC a t g a a c t g c a a g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g c g t t c c t t g c g t a c t t g a c g t t c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c c g c a a g g a a c g c

4560 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR 80 85 90 95 AAVLDVVTEAGRDWLLLGEV NeoR/KanR AAVLDVVTEAGRDWLLLGEV c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t t g g g c g a a g t g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a a c c c g c t t c a c g

4620 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR 100 105 110 115 PGQDLLSSHLAPAEKVSIMA NeoR/KanR PGQDLLSSHLAPAEKVSIMA *RVKSRGLFYGDHS c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t c c a t c a t g g c t g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a g g t a g t a c c g a c

BsrDI 4680 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR 120 125 130 135 DAMRRLHTLDPATCPFDHQA NeoR/KanR DAMRRLHTLDPATCPFDHQA ICHPPQMRKIRSGAWEVVLR a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g a c c a c c a a g c g a t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c t g g t g g t t c g c t

4740 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR 140 145 150 155 KHRIERARTRMEAGLVDQDD NeoR/KanR KHRIERARTRMEAGLVDQDD FMADLSCTSPHFGTKDILII a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g a t c a g g a t g a t c t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c t a g t c c t a c t a g

4800 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR 160 165 170 175 LDEEHQGLAPAELFARLKAS NeoR/KanR LDEEHQGLAPAELFARLKAS QVFLMLPERWGFQEGPELRA t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t c a a g g c g a g c a a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g a g t t c c g c t c g t

4860 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR 180 185 190 195 MPDGEDLVVTHGDACLPNIM NeoR/KanR MPDGEDLVVTHGDACLPNIM HGVALIEDHGMAIGAQRIDH t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c c g a a t a t c a t g g a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g g c t t a t a g t a c c

RsrII 4920 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR 200 205 210 215 VENGRFSGFIDCGRLGVADR NeoR/KanR VENGRFSGFIDCGRLGVADR HFIAAKRSEDVTAPQTHRVA t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g t g g c g g a c c g c t a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c a c c g c c t g g c g a

4980 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR 220 225 230 235 YQDIALATRDIAEELGGEWA NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F YQDIALATRDIAEELGGEWA ILVYRQSGTINSFLKAAFPS a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g g c g a a t g g g c t g t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c c g c t t a c c c g a c

5040 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR 240 245 250 255 DRFLVLYGIAAPDSQRIAFY NeoR/KanR DRFLVLYGIAAPDSQRIAFY VAEEHKVTDGSGIRLADGEI a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a t c g c c t t c t a t c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t a g c g g a a g a t a g

5100 260 265 RLLDEFF* NeoR/KanR 260 265 RLLDEFF* NeoR/KanR MTDQAT RLLDEFF* AKKVLEESRSEPTRFSRGLS g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c c g a c c a a g c g a c c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g g c t g g t t c g c t g

5160 PNLPSRDFDSTAAFYERLGF AWGAM g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t g g g c t t c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a c c c g a a

5220 GIVFRDAGWMILQRGDLMLE c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t g c t g g a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a c g a c c t

5280 A C T T T G T G C C T T C C T C T G T T TK-pA-R FFAHPRGRLTETRKETIPEG g t t c t t c g c c c a c c c t a g g g g g a g g c t a a c t g a a a c a c g g a a g g a g a c a a t a c c g g a a g g c a a g a a g c g g g t g g g a t c c c c c t c c g a t t g a c t t t g t g c c t t c c t c t g t t a t g g c c t t c c

5340 HSV TK poly(A) signal TRAMTAIKRQNKTHGVGSFV a a c c c g c g c t a t g a c g g c a a t a a a a a g a c a g a a t a a a a c g c a c g g t g t t g g g t c g t t t g t t t g g g c g c g a t a c t g c c g t t a t t t t t c t g t c t t a t t t t g c g t g c c a c a a c c c a g c a a a c a

5400 HSV TK poly(A) signal HKRGVRSQGWHSVDTPPRPH t c a t a a a c g c g g g g t t c g g t c c c a g g g c t g g c a c t c t g t c g a t a c c c c a c c g a g a c c c c a a g t a t t t g c g c c c c a a g c c a g g g t c c c g a c c g t g a g a c a g c t a t g g g g t g g c t c t g g g g t

5460 WGQYARVSSFSPPHPPSSGE t t g g g g c c a a t a c g c c c g c g t t t c t t c c t t t t c c c c a c c c c a c c c c c c a a g t t c g g g t g a a a c c c c g g t t a t g c g g g c g c a a a g a a g g a a a a g g g g t g g g g t g g g g g g t t c a a g c c c a c t

5520 GPGLAANVGAAGPAIASGYS a g g c c c a g g g c t c g c a g c c a a c g t c g g g g c g g c a g g c c c t g c c a t a g c c t c a g g t t a c t c t c c g g g t c c c g a g c g t c g g t t g c a g c c c c g c c g t c c g g g a c g g t a t c g g a g t c c a a t g a g

5580 YIL* a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a

5640 c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g

5700 ori a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c

5760 ori c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a

5820 ori a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t t c t t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a a g a

5880 ori t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a

5940 ori c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c

6000 ori g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g

ApaLI 6060 ori g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t

6120 ori g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c

6180 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t

6240 ori t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c

6300 ori g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c

PciI 6360 c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t c c c c t g a t t c t g t g g a t a a c c g t a t g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a g g g g a c t a a g a c a c c t a t t g g c a t a

AseI 6420 t a c c g c c a t g c a t t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t g g c g g t a c g t a a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a

3ʹ 5ʹ 6433 a t a t g g a g t t c c g t a t a c c t c a a g g c

Restriction Enzymes

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BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

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  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.