Addgene EF1A_Hygro_P2A_RUNX1_E116V_Barcode Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: EF1A_Hygro_P2A_RUNX1_E116V_Barcode Sequencing Result


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F1ori-R (10,665 .. 10,684) BGH-rev (10,302 .. 10,319) PmeI (10,286) loxP BstXI (9798) AfeI (9670) BstEII (9480) PflMI * (9264) XcmI (9233) Bsu36I (8884) FspAI (8674) MreI (8590) BlpI (8542) BsaBI (8488) RsrII (7805) AsiSI (7761) BsmBI - Esp3I (7465) T7 (7315 .. 7334) EF1a-F (7246 .. 7266) PaeR7I - XhoI (7085) PasI (6868) AgeI (6198) BspDI - ClaI (6029) PacI (6025) KflI - PpuMI (5738) F1ori-F (11 .. 32) pBABE 3' (151 .. 171) MscI (602) MauBI (635) SV40pA-R (1140 .. 1159) EBV-rev (1194 .. 1213) BstZ17I (1235) M13 rev M13 Reverse (1273 .. 1289) lac operator M13/pUC Reverse (1286 .. 1308) lac promoter CAP binding site L4440 (1504 .. 1521) pBR322ori-F (1755 .. 1774) AhdI (2507) Amp-R (3057 .. 3076) SspI (3311) AmpR promoter SgrDI (3428) pRS-marker (3474 .. 3493) MluI (3658) SpeI (3679) SnaBI (4020) CMV-F (4199 .. 4219) NotI (4952) AleI (5381) EF1A_Hygro_P2A_RUNX1_E116V_Barcode 10,864 bp

Sequence View

5ʹ 3ʹ 60 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a

120 f1 ori t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a

180 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t g t g g a a a g t a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a c a c c t t t c a

240 SV40 promoter c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t

300 SV40 promoter g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a

360 SV40 promoter SV40 ori a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a

420 SV40 promoter SV40 ori c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c

480 SV40 promoter SV40 ori c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g c t t t t g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c g a a a a

540 SV40 promoter EM7 promoter g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a g c a c g t g t t g a c a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t c g t g c a c a a c t g t t

600 1 M EM7 promoter BleoR t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g g a a c t a a a c c a t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c c t t g a t t t g g t a

MauBIMscI 660 5 10 15 20 AKLTSAVPVLTARDVAGAVE BleoR g g c c a a g t t g a c c a g t g c c g t t c c g g t g c t c a c c g c g c g c g a c g t c g c c g g a g c g g t c g a c c g g t t c a a c t g g t c a c g g c a a g g c c a c g a g t g g c g c g c g c t g c a g c g g c c t c g c c a g c t

720 25 30 35 40 FWTDRLGFSRDFVEDDFAGV BleoR g t t c t g g a c c g a c c g g c t c g g g t t c t c c c g g g a c t t c g t g g a g g a c g a c t t c g c c g g t g t c a a g a c c t g g c t g g c c g a g c c c a a g a g g g c c c t g a a g c a c c t c c t g c t g a a g c g g c c a c a

780 45 50 55 60 VRDDVTLFISAVQDQVVPDN BleoR g g t c c g g g a c g a c g t g a c c c t g t t c a t c a g c g c g g t c c a g g a c c a g g t g g t g c c g g a c a a c c a g g c c c t g c t g c a c t g g g a c a a g t a g t c g c g c c a g g t c c t g g t c c a c c a c g g c c t g t t

840 65 70 75 80 TLAWVWVRGLDELYAEWSEV BleoR c a c c c t g g c c t g g g t g t g g g t g c g c g g c c t g g a c g a g c t g t a c g c c g a g t g g t c g g a g g t g t g g g a c c g g a c c c a c a c c c a c g c g c c g g a c c t g c t c g a c a t g c g g c t c a c c a g c c t c c a

900 85 90 95 100 VSTNFRDASGPAMTEIGEQP BleoR c g t g t c c a c g a a c t t c c g g g a c g c c t c c g g g c c g g c c a t g a c c g a g a t c g g c g a g c a g c c g c a c a g g t g c t t g a a g g c c c t g c g g a g g c c c g g c c g g t a c t g g c t c t a g c c g c t c g t c g g

960 105 110 115 120 WGREFALRDPAGNCVHFVAE BleoR g t g g g g g c g g g a g t t c g c c c t g c g c g a c c c g g c c g g c a a c t g c g t g c a c t t c g t g g c c g a c a c c c c c g c c c t c a a g c g g g a c g c g c t g g g c c g g c c g t t g a c g c a c g t g a a g c a c c g g c t

1020 125 EQD* BleoR g g a g c a g g a c t g a c a c g t g c t a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a a a g g t t c c t c g t c c t g a c t g t g c a c g a t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t t t c c a a

1080 g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t c t c a t c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t a g a g t a

1140 SV40 poly(A) signal C SV40pA-R g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c

1200 SV40 poly(A) signal G T G G T T T EBV-rev G T T A T C G T A G T G T T T A A A G SV40pA-R c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a

BstZ17I 1260 SV40 poly(A) signal G T C C A A A C T C A T C EBV-rev g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a g a g c t t c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t c t c g a a

1320 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t

1380 -10 -35 lac promoter CAP binding site c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a

1440 CAP binding site c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a

1500 g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g

1560 G A G C G A G T G A C T G A G C G A L4440 t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a g c t c a a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g t c g a g t

1620 c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c

1680 ori a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t

1740 ori t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t

1800 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g

1860 ori t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g

1920 ori g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a

1980 ori g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c

2040 ori t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c

2100 ori g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t

2160 ori c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c

2220 ori a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a

2280 ori t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a

2340 t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c

2400 a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a

2460 285 280 *WHKILSAG AmpR *WHKILSAG c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g

AhdI 2520 275 270 265 260 IEAIQRNREDMTAQSGTTYI AmpR IEAIQRNREDMTAQSGTTYI t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a

2580 255 250 245 240 VVIRSPKGDPGLAAIIGRSG AmpR VVIRSPKGDPGLAAIIGRSG a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a c c c t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t g g g

2640 235 230 225 220 REGAGSKDAIFWGAPLASRL AmpR REGAGSKDAIFWGAPLASRL a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c

2700 215 210 205 200 LPGAVKDAEMWDILQQRSAL AmpR LPGAVKDAEMWDILQQRSAL a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c

2760 195 190 185 180 TLLEGTLLKRLTTAMAVPMT AmpR TLLEGTLLKRLTTAMAVPMT a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a

2820 175 170 165 160 TDREDNPIAENLEPEWRDLR AmpR TDREDNPIAENLEPEWRDLR g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c

2880 155 150 145 140 TVHDGMNHLFATLEKPGGIT AmpR TVHDGMNHLFATLEKPGGIT a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a

2940 135 130 125 120 TLLLNAATNDSMTIAASCLE AmpR TLLLNAATNDSMTIAASCLE t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g

3000 115 110 105 100 RVTMGDTLHKETVPSYEVLD AmpR RVTMGDTLHKETVPSYEVLD t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g

3060 95 90 85 80 NQSYHIRRGLQEQGADIRSL AmpR A T A A Amp-R NQSYHIRRGLQEQGADIRSL a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t

3120 75 70 65 60 VAGCLLVKFTSMMPFREEPR AmpR T A C C G C G C C A C A T A G C Amp-R VAGCLLVKFTSMMPFREEPR t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c

3180 55 50 45 40 FSELIKGSNLDLEIYGVRAG AmpR FSELIKGSNLDLEIYGVRAG a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g

3240 35 30 25 LQDEADKVKVLTEPH 20 signal sequence AFVPL AmpR LQDEADKVKVLTEPHAFVPL c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c

3300 15 10 5 1 signal sequence CFAAFFPILAVRFHQISM AmpR AmpR promoter CFAAFFPILAVRFHQISM g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a

SspI 3360 AmpR promoter c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a

3420 AmpR promoter t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g

SgrDI 3480 A T G T T A G pRS-marker a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g

3540 A C G A G A C T A C G G C pRS-marker t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a

3600 g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c

MluI 3660 a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c

SpeI 3720 CMV enhancer c g t t g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t g c a a c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a

3780 CMV enhancer a g c c c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g t c g g g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c

3840 CMV enhancer c c c a a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g t t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t

3900 CMV enhancer g g g a c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c c c t g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t

3960 CMV enhancer c a t c a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g t a g t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g

SnaBI 4020 CMV enhancer g c c t g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c c g g a c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g

4080 CMV enhancer CMV promoter g t a t t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a c a t a a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t

4140 CMV promoter t a g c g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g a t c g c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c

4200 CMV promoter C G CMV-F t t t t g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g a a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c

4260 CMV promoter C A A A T G G G C G G T A G G C G T G CMV-F c a a a t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c g t t t a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g

4320 5' LTR (truncated) t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g

4380 5' LTR (truncated) a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a

4440 5' LTR (truncated) g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c

4500 5' LTR (truncated) HIV-1 Ψ c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g

4560 HIV-1 Ψ a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c

4620 HIV-1 Ψ c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a

4680 a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t

4740 a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a

4800 c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g

4860 c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a

4920 g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c

NotI 4980 c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c

5040 MRDNWRSELYKYKVVKIEP a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g

5100 LGVAPTKAKRRVVQREKRAV a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a

5160 RRE GIGALFLGFLGAAGSTMGAA g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g

5220 RRE SMTLTVQARQLLSGIVQQQN g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t

5280 RRE NLLRAIEAQQHLLQLTVWGI c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a

5340 RRE KQLQARILAVERYLKDQQLL c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a

AleI 5400 GIWGCSGKLICTTAVPWNAS g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c

5460 WSNKSLEQIWNHTTWMEWDR t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c

5520 EINNYTSLIHSLIEESQNQQ a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t

5580 1 5 10 15 KNEQELLELDKWASL WNWF gp41 peptide EKNEQELLELDKWASLWNWF a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a

5640 (in frame with gp41 peptide) NITNWLWYIKLFIMIVGGLV NITNWLWYIKLFIMIVGGLV t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a

5700 (in frame with gp41 peptide) GLRIVFAVLSIVNRVRQGYS GLRIVFAVLSIVNRVRQGYS a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g

PpuMIKflI 5760 (in frame with gp41 peptide) PLSFQTHLPTPRGPDRPEGI PLSFQTHLPTPRGPDRPEGI a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a

5820 (in frame with gp41 peptide) EEEGGERDRDRSIRLVNGSA EEEGGERDRDRSIRLVNGSA a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g

5880 (in frame with gp41 peptide) LRAPILQTNGSIHPQF* cPPT/CTS LRAPILQTNGSIHPQF* a c t g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g t g a c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c

5940 cPPT/CTS g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g

6000 cPPT/CTS a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c

BspDIClaIPacI 6060 a c a g c a g a g a t c c a g t t t g g t t a a t t a a t c g a t a a g c t t t g c a a a g a t g g a t a a a g t t t t t g t c g t c t c t a g g t c a a a c c a a t t a a t t a g c t a t t c g a a a c g t t t c t a c c t a t t t c a a a a

6120 EF-1 α promoter a a a c a g a g a g g a a t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g t t t g t c t c t c c t t a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c

6180 EF-1 α promoter g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c

AgeI 6240 EF-1 α promoter g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c

6300 EF-1 α promoter a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c

6360 EF-1 α promoter EF-1 α intron A t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c

6420 EF-1 α promoter EF-1 α intron A * t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t

6480 EF-1 α promoter EF-1 α intron A KWRAATRSEQDRAEPQFHTP c t t c c a c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g g a a g g t g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c

6540 EF-1 α promoter EF-1 α intron A SNSAKRKLLGKAEHKLQPRA a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g

6600 EF-1 α promoter EF-1 α intron A QASPGGRAFRTAGERRDRQK c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a

6660 EF-1 α promoter EF-1 α intron A RYTELWKFNKIVQQSAKKRA t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g

6720 EF-1 α promoter EF-1 α intron A LYDQLHPGLDACQYKPKQPR a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c

6780 EF-1 α promoter EF-1 α intron A PRRRPGHTGACMNPSAPGAL c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c

6840 EF-1 α promoter EF-1 α intron A AAVSFRVPTTELQGAQEPAQ c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c

PasI 6900 EF-1 α promoter EF-1 α intron A GRAATYRGARPPLAPGTPVL g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c

6960 EF-1 α promoter EF-1 α intron A QTLPFIAAERGQQLSSLISS t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t

7020 EF-1 α promoter EF-1 α intron A AASPLAPPHTVWVFSFPRET c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a

7080 EF-1 α promoter EF-1 α intron A RLRRKM c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a

PaeR7IXhoI 7140 EF-1 α promoter EF-1 α intron A a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c

7200 EF-1 α promoter EF-1 α intron A a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a

7260 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G EF1a-F t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c

7320 EF-1 α promoter T7 promoter EF-1 α intron A T G G T T C EF1a-F T A A T A C T7 *TDHPILKTSIR t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t a g c t t g g t a c t a a t a c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a t c g a a c c a t g a t t a t g

7380 T7 promoter G A C T C A C T A T A G G G T7 SVIPLGLSALYAQYRIGAAR g a c t c a c t a t a g g g a g a c c c a a g c t g g c t a g g t a a g c t t g a t a t c g a a t t c c t g c a g c c c c t g a g t g a t a t c c c t c t g g g t t c g a c c g a t c c a t t c g a a c t a t a g c t t a a g g a c g t c g g g

7440 1 5 10 15 MKKPELTATSVEKFL HygR 1 5 10 15 MKKPELTATSVEKFL HygR MKKPELTATSVEKFL PIGGGHFLRFEGRRRDLLKQ g g g g g a t c c c g c c a c c a t g a a a a a g c c t g a a c t c a c c g c g a c g t c t g t c g a g a a g t t t c t c c c c c t a g g g c g g t g g t a c t t t t t c g g a c t t g a g t g g c g c t g c a g a c a g c t c t t c a a a g a

BsmBIEsp3I 7500 20 25 30 35 IEKFDSVSDLMQLSEGEESR HygR 20 25 30 35 IEKFDSVSDLMQLSEGEESR HygR IEKFDSVSDLMQLSEGEESR DFLEVADGVQHLERLAFFRT g a t c g a a a a g t t c g a c a g c g t c t c c g a c c t g a t g c a g c t c t c g g a g g g c g a a g a a t c t c g c t a g c t t t t c a a g c t g t c g c a g a g g c t g g a c t a c g t c g a g a g c c t c c c g c t t c t t a g a g c

7560 40 45 50 55 AFSFDVGGRGYVLRVNSCAD HygR 40 45 50 55 AFSFDVGGRGYVLRVNSCAD HygR AFSFDVGGRGYVLRVNSCAD SEAEIYSPTSIDQPYIAAGI t g c t t t c a g c t t c g a t g t a g g a g g g c g t g g a t a t g t c c t g c g g g t a a a t a g c t g c g c c g a a c g a a a g t c g a a g c t a c a t c c t c c c g c a c c t a t a c a g g a c g c c c a t t t a t c g a c g c g g c t

7620 60 65 70 75 GFYKDRYVYRHFASAALPIP HygR 60 65 70 75 GFYKDRYVYRHFASAALPIP HygR GFYKDRYVYRHFASAALPIP TEVFITINIPVKCRGRERNR t g g t t t c t a c a a a g a t c g t t a t g t t t a t c g g c a c t t t g c a t c g g c c g c g c t c c c g a t t c c a c c a a a g a t g t t t c t a g c a a t a c a a a t a g c c g t g a a a c g t a g c c g g c g c g a g g g c t a a g g

7680 80 85 90 95 EVLDIGEFSESLTYCISRRA HygR 80 85 90 95 EVLDIGEFSESLTYCISRRA HygR EVLDIGEFSESLTYCISRRA FHKVNPFEALAQGIADGATC g g a a g t g c t t g a c a t t g g g g a a t t c a g c g a g a g c c t g a c c t a t t g c a t c t c c c g c c g t g c c c t t c a c g a a c t g t a a c c c c t t a a g t c g c t c t c g g a c t g g a t a a c g t a g a g g g c g g c a c g

7740 100 105 110 115 QGVTLQDLPETELPAVLQPV HygR 100 105 110 115 QGVTLQDLPETELPAVLQPV HygR QGVTLQDLPETELPAVLQPV LTDRQLVQRFGFQGSNQLRD a c a g g g t g t c a c g t t g c a a g a c c t g c c t g a a a c c g a a c t g c c c g c t g t t c t g c a g c c g g t t g t c c c a c a g t g c a a c g t t c t g g a c g g a c t t t g g c t t g a c g g g c g a c a a g a c g t c g g c c a

AsiSI 7800 120 125 130 135 AEAMDAIAAADLSQTSGFGP HygR 120 125 130 135 AEAMDAIAAADLSQTSGFGP HygR AEAMDAIAAADLSQTSGFGP RLGHIRDSRGIKALRAPEAW c g c g g a g g c c a t g g a t g c g a t c g c t g c g g c c g a t c t t a g c c a g a c g a g c g g g t t c g g c c c g c g c c t c c g g t a c c t a c g c t a g c g a c g c c g g c t a g a a t c g g t c t g c t c g c c c a a g c c g g g

RsrII 7860 140 145 150 155 FGPQGIGQYTTWRDFICAIA HygR 140 145 150 155 FGPQGIGQYTTWRDFICAIA HygR FGPQGIGQYTTWRDFICAIA ESRLSDTLVSCPTIEYARNS a t t c g g a c c g c a a g g a a t c g g t c a a t a c a c t a c a t g g c g t g a t t t c a t a t g c g c g a t t g c t a a g c c t g g c g t t c c t t a g c c a g t t a t g t g a t g t a c c g c a c t a a a g t a t a c g c g c t a a c g

7920 160 165 170 175 DPHVYHWQTVMDDTVSASVA HygR 160 165 170 175 DPHVYHWQTVMDDTVSASVA HygR DPHVYHWQTVMDDTVSASVA IGMHIVPLSHHVVGDTRGDR t g a t c c c c a t g t g t a t c a c t g g c a a a c t g t g a t g g a c g a c a c c g t c a g t g c g t c c g t c g c a c t a g g g g t a c a c a t a g t g a c c g t t t g a c a c t a c c t g c t g t g g c a g t c a c g c a g g c a g c g

7980 180 185 190 195 QALDELMLWAEDCPEVRHLV HygR 180 185 190 195 QALDELMLWAEDCPEVRHLV HygR QALDELMLWAEDCPEVRHLV LSEILQHKPGLVAGFDPVEH g c a g g c t c t c g a t g a g c t g a t g c t t t g g g c c g a g g a c t g c c c c g a a g t c c g g c a c c t c g t c g t c c g a g a g c t a c t c g a c t a c g a a a c c c g g c t c c t g a c g g g g c t t c a g g c c g t g g a g c a

8040 200 205 210 215 HADFGSNNVLTDNGRITAVI HygR 200 205 210 215 HADFGSNNVLTDNGRITAVI HygR HADFGSNNVLTDNGRITAVI VRIEAGVIDQRVIAAYCRDN g c a c g c g g a t t t c g g c t c c a a c a a t g t c c t g a c g g a c a a t g g c c g c a t a a c a g c g g t c a t c g t g c g c c t a a a g c c g a g g t t g t t a c a g g a c t g c c t g t t a c c g g c g t a t t g t c g c c a g t a

8100 220 225 230 235 DWSEAMFGDSQYEVANIFFW HygR 220 225 230 235 DWSEAMFGDSQYEVANIFFW HygR DWSEAMFGDSQYEVANIFFW VPALRHEPIGLVLDGVDEEP t g a c t g g a g c g a g g c g a t g t t c g g g g a t t c c c a a t a c g a g g t c g c c a a c a t c t t c t t c t g a c t g a c c t c g c t c c g c t a c a a g c c c c t a a g g g t t a t g c t c c a g c g g t t g t a g a a g a a g a c

8160 240 245 250 255 RPWLACMEQQTRYFERRHPE HygR 240 245 250 255 RPWLACMEQQTRYFERRHPE HygR RPWLACMEQQTRYFERRHPE PRPQSTHLLLRAVELPPM g a g g c c g t g g t t g g c t t g t a t g g a g c a g c a g a c g c g c t a c t t c g a g c g g a g g c a t c c g g a c t c c g g c a c c a a c c g a a c a t a c c t c g t c g t c t g c g c g a t g a a g c t c g c c t c c g t a g g c c t

8220 260 265 270 275 LAGSPRLRAYMLRIGLDQLY HygR 260 265 270 275 LAGSPRLRAYMLRIGLDQLY HygR LAGSPRLRAYMLRIGLDQLY g c t t g c a g g a t c g c c g c g g c t c c g g g c g t a t a t g c t c c g c a t t g g t c t t g a c c a a c t c t a c g a a c g t c c t a g c g g c g c c g a g g c c c g c a t a t a c g a g g c g t a a c c a g a a c t g g t t g a g a t

8280 280 285 290 295 QSLVDGNFDDAAWAQGRCDA HygR 280 285 290 295 QSLVDGNFDDAAWAQGRCDA HygR QSLVDGNFDDAAWAQGRCDA t c a g a g c t t g g t t g a c g g c a a t t t c g a t g a t g c a g c t t g g g c g c a g g g t c g a t g c g a c g c a g t c t c g a a c c a a c t g c c g t t a a a g c t a c t a c g t c g a a c c c g c g t c c c a g c t a c g c t g c g

8340 300 305 310 315 IVRSGAGTVGRTQIARRSAA HygR 300 305 310 315 IVRSGAGTVGRTQIARRSAA HygR IVRSGAGTVGRTQIARRSAA a a t c g t c c g a t c c g g a g c c g g g a c t g t c g g g c g t a c a c a a a t c g c c c g c a g a a g c g c g g c t t a g c a g g c t a g g c c t c g g c c c t g a c a g c c c g c a t g t g t t t a g c g g g c g t c t t c g c g c c g

8400 320 325 330 335 VWTDGCVEVLADSGNRRPST HygR 320 325 330 335 VWTDGCVEVLADSGNRRPST HygR VWTDGCVEVLADSGNRRPST c g t c t g g a c c g a t g g c t g t g t a g a a g t a c t c g c c g a t a g t g g a a a c c g a c g c c c c a g c a c g c a g a c c t g g c t a c c g a c a c a t c t t c a t g a g c g g c t a t c a c c t t t g g c t g c g g g g t c g t g

8460 340 RPRAKE (in frame with HygR) GSGATNFSLLKQAG HygR 340 RPRAKE (in frame with HygR) GSGATNFSLLKQAG HygR RPRAKEGSGATNFSLLKQAG t c g t c c g a g g g c a a a g g a a g g a t c c g g c g c a a c a a a c t t c t c t c t g c t g a a a c a g g c c g g a g c a g g c t c c c g t t t c c t t c c t a g g c c g c g t t g t t t g a a g a g a g a c g a c t t t g t c c g g c c

BsaBI 8520 (in frame with HygR) DVKRILDRCVSP* (in frame with HygR) DVKRILDRCVSP* MRIPVDASTSRR DVKRILDRCVSP* a g a t g t c a a g a g a a t c c t g g a c c g a t g c g t a t c c c c g t a g a t g c c a g c a c g a g c c g c c g c t c t a c a g t t c t c t t a g g a c c t g g c t a c g c a t a g g g g c a t c t a c g g t c g t g c t c g g c g g c g

BlpI 8580 FTPPSTALSPGKMSEALPLG t t c a c g c c g c c t t c c a c c g c g c t g a g c c c a g g c a a g a t g a g c g a g g c g t t g c c g c t g g g c a a g t g c g g c g g a a g g t g g c g c g a c t c g g g t c c g t t c t a c t c g c t c c g c a a c g g c g a c c c g

MreI 8640 APDAGAALAGKLRSGDRSMV g c c c c g g a c g c c g g c g c t g c c c t g g c c g g c a a g c t g a g g a g c g g c g a c c g c a g c a t g g t g c g g g g c c t g c g g c c g c g a c g g g a c c g g c c g t t c g a c t c c t c g c c g c t g g c g t c g t a c c a c

FspAI 8700 EVLADHPGELVRTDSPNFLC g a g g t g c t g g c c g a c c a c c c g g g c g a g c t g g t g c g c a c c g a c a g c c c c a a c t t c c t c t g c c t c c a c g a c c g g c t g g t g g g c c c g c t c g a c c a c g c g t g g c t g t c g g g g t t g a a g g a g a c g

8760 SVLPTHWRCNKTLPIAFKVV t c c g t g c t g c c t a c g c a c t g g c g c t g c a a c a a g a c c c t g c c c a t c g c t t t c a a g g t g g t g a g g c a c g a c g g a t g c g t g a c c g c g a c g t t g t t c t g g g a c g g g t a g c g a a a g t t c c a c c a c

8820 ALGDVPDGTLVTVMAGNDEN g c c c t a g g g g a t g t t c c a g a t g g c a c t c t g g t c a c t g t g a t g g c t g g c a a t g a t g a a a a c c g g g a t c c c c t a c a a g g t c t a c c g t g a g a c c a g t g a c a c t a c c g a c c g t t a c t a c t t t t g

8880 YSAVLRNATAAMKNQVARFN t a c t c g g c t g t g c t g a g a a a t g c t a c c g c a g c c a t g a a g a a c c a g g t t g c a a g a t t t a a t a t g a g c c g a c a c g a c t c t t t a c g a t g g c g t c g g t a c t t c t t g g t c c a a c g t t c t a a a t t a

Bsu36I 8940 DLRFVGRSGRGKSFTLTITV g a c c t c a g g t t t g t c g g t c g a a g t g g a a g a g g g a a a a g c t t c a c t c t g a c c a t c a c t g t c c t g g a g t c c a a a c a g c c a g c t t c a c c t t c t c c c t t t t c g a a g t g a g a c t g g t a g t g a c a g

9000 FTNPPQVATYHRAIKITVDG t t c a c a a a c c c a c c g c a a g t c g c c a c c t a c c a c a g a g c c a t c a a a a t c a c a g t g g a t g g g a a g t g t t t g g g t g g c g t t c a g c g g t g g a t g g t g t c t c g g t a g t t t t a g t g t c a c c t a c c c

9060 PREPRRHRQKLDDQTKPGSL c c c c g a g a a c c t c g a a g a c a t c g g c a g a a a c t a g a t g a t c a g a c c a a g c c c g g g a g c t t g g g g g c t c t t g g a g c t t c t g t a g c c g t c t t t g a t c t a c t a g t c t g g t t c g g g c c c t c g a a c

9120 SFSERLSELEQLRRTAMRVS t c c t t t t c c g a g c g g c t c a g t g a a c t g g a g c a g c t g c g g c g c a c a g c c a t g a g g g t c a g c a g g a a a a g g c t c g c c g a g t c a c t t g a c c t c g t c g a c g c c g c g t g t c g g t a c t c c c a g t c g

9180 PHHPAPTPNPRASLNHSTAF c c a c a c c a c c c a g c c c c c a c g c c c a a c c c t c g t g c c t c c c t g a a c c a c t c c a c t g c c t t t g g t g t g g t g g g t c g g g g g t g c g g g t t g g g a g c a c g g a g g g a c t t g g t g a g g t g a c g g a a a

XcmI 9240 NPQPQSQMQDTRQIQPSPPW a a c c c t c a g c c t c a g a g t c a g a t g c a g g a t a c a a g g c a g a t c c a a c c a t c c c c a c c g t g g t t g g g a g t c g g a g t c t c a g t c t a c g t c c t a t g t t c c g t c t a g g t t g g t a g g g g t g g c a c c

PflMI* 9300 SYDQSYQYLGSIASPSVHPA t c c t a c g a t c a g t c c t a c c a a t a c c t g g g a t c c a t t g c c t c t c c t t c t g t g c a c c c a g c a a g g a t g c t a g t c a g g a t g g t t a t g g a c c c t a g g t a a c g g a g a g g a a g a c a c g t g g g t c g t

9360 TPISPGRASGMTTLSAELSS a c g c c c a t t t c a c c t g g a c g t g c c a g c g g c a t g a c a a c c c t c t c t g c a g a a c t t t c c a g t t g c g g g t a a a g t g g a c c t g c a c g g t c g c c g t a c t g t t g g g a g a g a c g t c t t g a a a g g t c a

9420 RLSTAPDLTAFSDPRQFPAL c g a c t c t c a a c g g c a c c c g a c c t g a c a g c g t t c a g c g a c c c g c g c c a g t t c c c c g c g c t g g c t g a g a g t t g c c g t g g g c t g g a c t g t c g c a a g t c g c t g g g c g c g g t c a a g g g g c g c g a c

9480 PSISDPRMHYPGAFTYSPTP c c c t c c a t c t c c g a c c c c c g c a t g c a c t a t c c a g g c g c c t t c a c c t a c t c c c c g a c g c c g g g g a g g t a g a g g c t g g g g g c g t a c g t g a t a g g t c c g c g g a a g t g g a t g a g g g g c t g c g g c

BstEII 9540 VTSGIGIGMSAMGSATRYHT g t c a c c t c g g g c a t c g g c a t c g g c a t g t c g g c c a t g g g c t c g g c c a c g c g c t a c c a c a c c c a g t g g a g c c c g t a g c c g t a g c c g t a c a g c c g g t a c c c g a g c c g g t g c g c g a t g g t g t g g

9600 YLPPPYPGSSQAQGGPFQAS t a c c t g c c g c c g c c c t a c c c c g g c t c g t c g c a a g c g c a g g g a g g c c c g t t c c a a g c c a g c a t g g a c g g c g g c g g g a t g g g g c c g a g c a g c g t t c g c g t c c c t c c g g g c a a g g t t c g g t c g

9660 SPSYHLYYGASAGSYQFSMV t c g c c c t c c t a c c a c c t g t a c t a c g g c g c c t c g g c c g g c t c c t a c c a g t t c t c c a t g g t g a g c g g g a g g a t g g t g g a c a t g a t g c c g c g g a g c c g g c c g a g g a t g g t c a a g a g g t a c c a c

AfeI 9720 GGERSPPRILPPCTNASTGS g g c g g c g a g c g c t c g c c g c c g c g c a t c c t g c c g c c c t g c a c c a a c g c c t c c a c c g g c t c c c c g c c g c t c g c g a g c g g c g g c g c g t a g g a c g g c g g g a c g t g g t t g c g g a g g t g g c c g a g g

9780 ALLNPSLPNQSDVVEAEGSH g c g c t g c t c a a c c c c a g c c t c c c g a a c c a g a g c g a c g t g g t g g a g g c c g a g g g c a g c c a c c g c g a c g a g t t g g g g t c g g a g g g c t t g g t c t c g c t g c a c c a c c t c c g g c t c c c g t c g g t g

BstXI 9840 SNSPTNMAPSARLEEAVWRP a g c a a c t c c c c c a c c a a c a t g g c g c c c t c c g c g c g c c t g g a g g a g g c c g t g t g g a g g c c c t c g t t g a g g g g g t g g t t g t a c c g c g g g a g g c g c g c g g a c c t c c t c c g g c a c a c c t c c g g g

9900 Y* t a c t g a c t a g c t a a g t t a g a a c t a t t t c c t g g c t g t t a c g c g c g t t t a t g g g c c c t a c t g a t g a c t g a t c g a t t c a a t c t t g a t a a a g g a c c g a c a a t g c g c g c a a a t a c c c g g g a t g a c

9960 a c g g g c t t g t t a a a c a g t g a a c c g c c c g c c c c a c g a c c c g c a g c g c c c g a c c g a a a g g a g t g c c c g a a c a a t t t g t c a c t t g g c g g g c g g g g t g c t g g g c g t c g c g g g c t g g c t t t c c t c

10,020 c g c a c g a c c c c a t g c a t c g a a c a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c g c g t g c t g g g g t a c g t a g c t t g t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g

10,080 loxP a a c g a a g a c a a g a t a t c a t a a c t t c g t a t a g c a t a c a t t a t a c g a a g t t a t c g t c t a g c t t t g c t t c t g t t c t a t a g t a t t g a a g c a t a t c g t a t g t a a t a t g c t t c a a t a g c a g a t c g a

10,140 5' LTR (truncated) g g t c c g g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c c c a g g c c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g

10,200 5' LTR (truncated) t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c

10,260 5' LTR (truncated) t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c

PmeI 10,320 5' LTR (truncated) bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev t g g a a a a t c t c t a g c a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g a c c t t t t a g a g a t c g t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c

10,380 bGH poly(A) signal t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g

10,440 bGH poly(A) signal t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t

10,500 bGH poly(A) signal t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c

10,560 bGH poly(A) signal c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t g g g g g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g a c c c c

10,620 f1 ori c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a

10,680 f1 ori G A G G A A A G C G A A A G A A F1ori-R t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a

10,740 f1 ori G G G A F1ori-R c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g

10,800 f1 ori t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t

10,860 f1 ori t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g

3ʹ 5ʹ 10,864 f1 ori c a c g g t g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.