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Sequence Analyzer: pTK Sequencing Result


Map View

BspQI - SapI (14) CAP binding site M13/pUC Reverse (3991 .. 4013) lac operator M13 Reverse (3978 .. 3994) SP6 (3943 .. 3960) NsiI (3929) BstXI (3920) MluI (3916) SacI (3911) Eco53kI (3909) NdeI (3899) HincII (3894) SalI (3892) PstI - SbfI (3890) BfuAI - BspMI (3879) SpeI (3866) HindIII (3838) BsgI (3771) BsaBI * (3694) PmlI (3677) EcoO109I * (3638) BspEI (3495) EcoNI (3333) XcmI (3317) BsmI (3100) PluTI (2986) SfoI (2984) NarI (2983) KasI (2982) PflFI - Tth111I (2940) BspDI - ClaI (2869) BstAPI (2843) AatII (2648) ZraI (2646) T7 (2612 .. 2631) M13 fwd M13 Forward (2588 .. 2605) M13/pUC Forward (2574 .. 2596) F1ori-R (2342 .. 2361) DraIII (2217) F1ori-F (2130 .. 2151) L4440 (20 .. 37) PciI (130) pBR322ori-F (271 .. 290) AlwNI (546) AhdI (1023) BpmI (1093) ScaI (1503) Amp-R (1573 .. 1592) XmnI (1622) PsiI (2089) pTK 4189 bp

Sequence View

BspQISapI 60 G A G C G A G T G A C T G A G C G A L4440 t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g

120 g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c

PciI 180 c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a

240 ori t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t

300 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a

360 ori c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g

420 ori c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c

480 ori t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a

540 ori t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c

AlwNI 600 ori c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t

660 ori a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t

720 ori a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c

780 ori g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c

840 a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c

900 g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a

960 285 *WHK AmpR *WHK g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a

1020 280 275 270 265 ILSAGIEAIQRNREDMTAQS AmpR ILSAGIEAIQRNREDMTAQS t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g

AhdI 1080 260 255 250 245 GTTYIVVIRSPKGDPGLAAI AmpR GTTYIVVIRSPKGDPGLAAI t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t

BpmI 1140 240 235 230 225 IGRSGREGAGSKDAIFWGAP AmpR IGRSGREGAGSKDAIFWGAP t g a t a c c g c g c g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g a c t a t g g c g c g c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c

1200 220 215 210 205 LASRLLPGAVKDAEMWDILQ AmpR LASRLLPGAVKDAEMWDILQ g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a

1260 200 195 190 185 QRSALTLLEGTLLKRLTTAM AmpR QRSALTLLEGTLLKRLTTAM g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t

1320 180 175 170 165 AVPMTTDREDNPIAENLEPE AmpR AVPMTTDREDNPIAENLEPE t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a

1380 160 155 150 145 WRDLRTVHDGMNHLFATLEK AmpR WRDLRTVHDGMNHLFATLEK c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a

1440 140 135 130 125 PGGITTLLLNAATNDSMTIA AmpR PGGITTLLLNAATNDSMTIA t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c

1500 120 115 110 105 ASCLERVTMGDTLHKETVPS AmpR ASCLERVTMGDTLHKETVPS c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c

ScaI 1560 100 95 90 85 YEVLDNQSYHIRRGLQEQGA AmpR YEVLDNQSYHIRRGLQEQGA a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c

1620 80 75 70 65 DIRSLVAGCLLVKFTSMMPF AmpR A T A A T A C C G C G C C A C A T A G C Amp-R DIRSLVAGCLLVKFTSMMPF c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t

XmnI 1680 60 55 50 45 REEPRFSELIKGSNLDLEIY AmpR REEPRFSELIKGSNLDLEIY a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a

1740 40 35 30 25 GVRAGLQDEADKVKVLTEPH AmpR GVRAGLQDEADKVKVLTEPH a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a

1800 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFHQ AmpR AFVPLCFAAFFPILAVRFHQ g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a

1860 1 signal sequence ISM AmpR AmpR promoter ISM g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t

1920 AmpR promoter t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a

1980 t t c c c c g a a a a g t g c c a c c t g a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a a a g g g g c t t t t c a c g g t g g a c t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t

2040 f1 ori t a c c g c a t c a g g a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t g t t a t g g c g t a g t c c t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a c a a

PsiI 2100 f1 ori a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a a a g t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t t t c

2160 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a a g a t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t t c t

DraIII 2220 f1 ori a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g c t g g c c c a c t a c g t g t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c g a c c g g g t g a t g c a c

2280 f1 ori a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a a c c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t t g g

2340 f1 ori c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a a g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t t c c

2400 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t g c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g a c g

2460 f1 ori g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c a t t c g c c a t t c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g t a a g c g g t a a

2520 *AACSNPLAIPAPRKAIVGA c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a g c t g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t c g a

2580 C C C A G T C M13/pUC Forward PSLPIHQLAILNPLALTKGT g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a g t c c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t c a g

2640 M13 fwd T7 promoter A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A C T C A C T A T A G G G T7 VVNYFSPWHITIRSVIPRIP a c g a c g t t g t a a a a c g a c g g c c a g t g a a t t g t a a t a c g a c t c a c t a t a g g g c g a a t t g g g t g c t g c a a c a t t t t g c t g c c g g t c a c t t a a c a t t a t g c t g a g t g a t a t c c c g c t t a a c c c

ZraIAatII 2700 Kozak sequence Kozak sequence MLPAAMAAAGIRLGDE GSTAHERGGHRGRSNSQAIL c c c g a c g t c g c a t g c t c c c g g c c g c c a t g g c g g c c g c g g g a a t t c g a t t g g g c g a t g a g t g g g c t g c a g c g t a c g a g g g c c g g c g g t a c c g c c g g c g c c c t t a a g c t a a c c c g c t a c t c a

2760 WSRMASYPCHQHASAFDQAA PRSHGAVGAMLVGAGEILSG g g t c t c g a a t g g c c a g c t a c c c c t g c c a t c a g c a c g c c a g c g c c t t c g a t c a g g c t g c c c c c a g a g c t t a c c g g t c g a t g g g g a c g g t a g t c g t g c g g t c g c g g a a g c t a g t c c g a c g g g

2820 RSRGHNNRRTALRPRRQQKA AAASVVVTAGGQAWAALLLG g c a g c c g c g g a c a c a a c a a c c g t c g c a c c g c c c t g c g c c c a c g c c g c c a g c a g a a g g c c a c g t c g g c g c c t g t g t t g t t g g c a g c g t g g c g g g a c g c g g g t g c g g c g g t c g t c t t c c g g t

BspDIClaIBstAPI 2880 TEVRLEQKMPTLLRVYIDGP GFDAQLLLHGCQQAHVDIAG c c g a a g t c c g c t t g g a g c a g a a g a t g c c c a c a c t g c t g c g c g t g t a c a t c g a t g g c c c c c g g c t t c a g g c g a a c c t c g t c t t c t a c g g g t g t g a c g a c g c g c a c a t g t a g c t a c c g g g g g

2940 HGMGKTTTTQLLVALGSRDD MAHALGGRGLQQDGQAAAVV a t g g c a t g g g c a a g a c c a c c a c g a c c c a a c t g c t g g t c g c c c t g g g c a g c c g c g a c g a c a t a c c g t a c c c g t t c t g g t g g t g c t g g g t t g a c g a c c a g c g g g a c c c g t c g g c g c t g c t g t

KasINarISfoIPluTITth111IPflFI 3000 IVYVPEPMTYWQVLGASETI DDVHGLGHGVPLDQAGGLCN t c g t c t a c g t g c c c g a g c c c a t g a c c t a c t g g c a a g t c c t g g g c g c c t c c g a g a c a a t t g a g c a g a t g c a c g g g c t c g g g t a c t g g a t g a c c g t t c a g g a c c c g c g g a g g c t c t g t t a a c

3060 ANIYTTQHRLDQGEISAGDA GVDVGGLVAQILALDTGAVG c c a a c a t c t a c a c c a c c c a g c a c c g c t t g g a t c a g g g c g a g a t c a g t g c c g g c g a c g c c g g g t t g t a g a t g t g g t g g g t c g t g g c g a a c c t a g t c c c g c t c t a g t c a c g g c c g c t g c g g c

BsmI 3120 AVVMTSAQITMGMPYAVTDA GHHHGAGLDGHSHGVGHGIG c c g t g g t g a t g a c c a g c g c c c a g a t c a c c a t g g g a a t g c c c t a c g c c g t g a c c g a t g c c g g g c a c c a c t a c t g g t c g c g g g t c t a g t g g t a c c c t t a c g g g a t g c g g c a c t g g c t a c g g c

3180 VLAPHIGGEAGSSHAPPPAL DQGGVNSALGAARMGWGWGQ t c c t g g c c c c c c a c a t t g g a g g c g a g g c c g g c a g c t c g c a t g c c c c a c c c c c a g c c c t g a a g g a c c g g g g g g t g t a a c c t c c g c t c c g g c c g t c g a g c g t a c g g g g t g g g g g t c g g g a c t

3240 TIFLDRHPIAFMLCYPAARY CDEQIAVRDGEHQAVWGGTI c a a t c t t c c t g g a t c g c c a c c c g a t c g c c t t c a t g c t g t g c t a c c c a g c c g c c c g t t a t c g t t a g a a g g a c c t a g c g g t g g g c t a g c g g a a g t a c g a c a c g a t g g g t c g g c g g g c a a t a g

3300 LMGSMTPQAVLAFVALIPPT QHAAHGGLGHQGEHGQDGRG t g a t g g g c a g c a t g a c c c c c c a g g c c g t g c t g g c c t t c g t g g c c c t g a t c c c c c c g a c c c a c t a c c c g t c g t a c t g g g g g g t c c g g c a c g a c c g g a a g c a c c g g g a c t a g g g g g g c t g g g

EcoNIXcmI 3360 LPGTNIVLGALPEDRHIDRL QGAGVDHQSGQGLIAVDVAQ t g c c c g g c a c c a a c a t c g t g c t g g g a g c c c t g c c c g a g g a t c g c c a c a t c g a c c g c c t g g a c g g g c c g t g g t t g t a g c a c g a c c c t c g g g a c g g g c t c c t a g c g g t g t a g c t g g c g g a c c

3420 AKRQRPGERLDLAMLAAIRR GLALTWSLAQIQGHQGGDAA c c a a g c g c c a g c g t c c a g g a g a g c g c c t g g a t c t g g c c a t g c t g g c c g c c a t c c g c c g c g g g t t c g c g g t c g c a g g t c c t c t c g c g g a c c t a g a c c g g t a c g a c c g g c g g t a g g c g g c g c

3480 VYGLLANTVRYLQGGGSWRE HVAQQGVGHAIKLSAAGPAL t g t a c g g c c t g c t g g c c a a c a c c g t g c g c t a t c t t c a a g g a g g c g g c t c c t g g c g c g a g g a c a t g c c g g a c g a c c g g t t g t g g c a c g c g a t a g a a g t t c c t c c g c c g a g g a c c g c g c t c c

BspEI 3540 DWGQLSGTAVPPQGAEPQSN IPALQGSRGHWGLSGLGLAI a t t g g g g c c a a c t g t c c g g a a c g g c c g t g c c a c c c c a g g g a g c c g a g c c c c a g a g c a a t g t a a c c c c g g t t g a c a g g c c t t g c c g g c a c g g t g g g g t c c c t c g g c t c g g g g t c t c g t t a c

3600 AGPRPHIGDTLFTLFRAPEL GAWAGVDAIGQKRQETGGLQ c c g g c c c a c g c c c c c a c a t c g g c g a t a c c c t g t t t a c g c t g t t c c g t g c c c c c g a g c t g c g g c c g g g t g c g g g g g t g t a g c c g c t a t g g g a c a a a t g c g a c a a g g c a c g g g g g c t c g a c g

EcoO109I* 3660 LAPNGDLYNVFAWALDVLAK QGRVAIQVIHEGPGQIHQRL t g g c c c c g a a c g g c g a t t t g t a c a a t g t g t t c g c c t g g g c c c t g g a t g t g c t g g c g a a g c a c c g g g g c t t g c c g c t a a a c a t g t t a c a c a a g c g g a c c c g g g a c c t a c a c g a c c g c t t c g

BsaBI*PmlI 3720 RLRPMHVFILDYDQSPAGCR TQAGHVHEDQIVILAWGSAA g t c t g c g c c c c a t g c a c g t g t t c a t c c t g g a t t a c g a t c a a a g c c c a g c c g g a t g c c g c g c a g a c g c g g g g t a c g t g c a c a a g t a g g a c c t a a t g c t a g t t t c g g g t c g g c c t a c g g c g c

BsgI 3780 DALLQLTSGMVQTHVTTPGS VGEQLQCAAHHLGM a c g c c c t c t t g c a a t t g a c a a g c g g c a t g g t g c a g a c c c a t g t g a c c a c c c c c g g c a g c a t g c g g g a g a a c g t t a a c t g t t c g c c g t a c c a c g t c t g g g t a c a c t g g t g g g g g c c g t c g t

HindIII 3840 IPTICDLARTFAREMGEAN* t c c c c a c a a t c t g c g a t c t g g c c c g c a c a t t c g c c c g c g a g a t g g g c g a g g c t a a t t a a g a g g g g t g t t a g a c g c t a g a c c g g g c g t g t a a g c g g g c g c t c t a c c c g c t c c g a t t a a t t c

NdeISbfIPstISalIHincIIBspMIBfuAISpeI 3900 c t t c g a g a c c a c t c a t c g c c c a a t c a c t a g t g a a t t c g c g g c c g c c t g c a g g t c g a c c a t g a a g c t c t g g t g a g t a g c g g g t t a g t g a t c a c t t a a g c g c c g g c g g a c g t c c a g c t g g t a

NsiIBstXIMluIEco53kISacI 3960 SP6 promoter G A T A T C A C A G T G G A T T T A SP6 a t g g g a g a g c t c c c a a c g c g t t g g a t g c a t a g c t t g a g t a t t c t a t a g t g t c a c c t a a a t t a c c c t c t c g a g g g t t g c g c a a c c t a c g t a t c g a a c t c a t a a g a t a t c a c a g t g g a t t t a

4020 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a

4080 -10 -35 lac promoter CAP binding site c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g

4140 CAP binding site t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g

4189 c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.