Addgene pHIV-Luc-ZsGreen Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pHIV-Luc-ZsGreen Sequencing Result


Map View

MluI (9315) pRS-marker (9131 .. 9150) SgrDI (9085) SspI (8968) Amp-R (8714 .. 8733) pBR322ori-F (7412 .. 7431) BspEI (7077) pBluescriptKS (6565 .. 6581) BspDI - ClaI (6563) PflFI - Tth111I (6089) BstXI (5854) PflMI (5721) IRES-F (5662 .. 5681) PaqCI (5607) PmlI (5584) IRES reverse (5435 .. 5452) AvrII (5419) BamHI (5261) SmaI (5254) TspMI - XmaI (5252) XbaI (5243) CsiI - SexAI * (4925) HpaI (4791) SpeI (15) CMV enhancer NdeI (250) SnaBI (356) CMV-F (535 .. 555) BssHII (855) KflI - PpuMI (2078) PasI (3135) PaeR7I - XhoI (3352) EF1a-F (3513 .. 3533) NotI (3567) EcoRI (3580) MreI (3649) BsaBI * (4033) BlpI (4540) pHIV-Luc-ZsGreen 9321 bp

Sequence View

SpeI 60 CMV enhancer g a c a t t g a t t a t t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c t g t a a c t a a t a a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g

120 CMV enhancer c a t a t a t g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a g t a t a t a c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t

180 CMV enhancer a c g a c c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a t g c t g g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t

240 CMV enhancer c t t t c c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c g a a a g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g

NdeI 300 CMV enhancer a a g t g t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t t t c a c a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a

SnaBI 360 CMV enhancer g g c a t t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t c c g t a a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a

420 CMV enhancer CMV promoter t a g t c a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c a t c a g t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g

480 CMV promoter g g t t t g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t c c a a a c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a

540 CMV promoter C G C A A A CMV-F g g c a c c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a c c g t g g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t

600 CMV promoter 5' LTR (truncated) T G G G C G G T A G G C G T G CMV-F t g g g c g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g a c c c g c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c

660 5' LTR (truncated) t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c

720 5' LTR (truncated) c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a

780 5' LTR (truncated) g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a

840 HIV-1 Ψ g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t

BssHII 900 HIV-1 Ψ c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t

960 HIV-1 Ψ a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g

1020 g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a

1080 a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c

1140 g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g

1200 *IMIYYLLLGRNHA a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g

1260 DFSLSLLCWPLKLCSLPLAF a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t

1320 CFYSWRVALPRGSIKLGPPP a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c c g g c c g c t g a t c t t c a g a c c t g g a g g a g g t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g g c c g g c g a c t a g a a g t c t g g a c c t c c t c c

1380 MRDNWRSELYKYKVVKIEP SILSLQLLSNYLYLTTFISG a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g

1440 LGVAPTKAKRRVVQREKRAV NPTAGVLAFLLTTCLSFLAT a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a

1500 RRE GIGALFLGFLGAAGSTMGAA PIPAKNRPNKPAAPLVIPAA g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g

1560 RRE SMTLTVQARQLLSGIVQQQN DIVSVTCALCNNDPITCCCF g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t

1620 RRE NLLRAIEAQQHLLQLTVWGI LKSLAISACCCRNCSVTQPM c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a

1680 RRE KQLQARILAVERYLKDQQLL c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a

1740 GIWGCSGKLICTTAVPWNAS g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c

1800 WSNKSLEQIWNHTTWMEWDR t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c

1860 EINNYTSLIHSLIEESQNQQ a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t

1920 1 5 10 15 KNEQELLELDKWASL WNWF gp41 peptide EKNEQELLELDKWASLWNWF a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a

1980 (in frame with gp41 peptide) NITNWLWYIKLFIMIVGGLV NITNWLWYIKLFIMIVGGLV t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a

2040 (in frame with gp41 peptide) GLRIVFAVLSIVNRVRQGYS GLRIVFAVLSIVNRVRQGYS a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g

PpuMIKflI 2100 (in frame with gp41 peptide) PLSFQTHLPTPRGPDRPEGI PLSFQTHLPTPRGPDRPEGI a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a

2160 (in frame with gp41 peptide) EEEGGERDRDRSIRLVNGSA EEEGGERDRDRSIRLVNGSA a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g

2220 (in frame with gp41 peptide) LRAPILQTNGSIHPQF* cPPT/CTS LRAPILQTNGSIHPQF* a c t g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g t g a c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c

2280 cPPT/CTS g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g

2340 cPPT/CTS a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c

2400 EF-1 α promoter a c a g c a g a g a t c c a g t t t g g t t a g t a c c g g g c c c g c t c t a g c g t g a g g c t c c g g t g c c c g t g t c g t c t c t a g g t c a a a c c a a t c a t g g c c c g g g c g a g a t c g c a c t c c g a g g c c a c g g g c

2460 EF-1 α promoter t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t

2520 EF-1 α promoter t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c

2580 EF-1 α promoter g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t

2640 EF-1 α promoter EF-1 α intron A c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c

2700 EF-1 α promoter EF-1 α intron A *KWRA c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a

2760 EF-1 α promoter EF-1 α intron A ATRSEQDRAEPQFHTPSNSA g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g g c c c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c c g g

2820 EF-1 α promoter EF-1 α intron A KRKLLGKAEHKLQPRAQASP t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c

2880 EF-1 α promoter EF-1 α intron A GGRAFRTAGERRDRQKRYTE c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a

2940 EF-1 α promoter EF-1 α intron A LWKFNKIVQQSAKKRALYDQ a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a

3000 EF-1 α promoter EF-1 α intron A LHPGLDACQYKPKQPRPRRR a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c

3060 EF-1 α promoter EF-1 α intron A PGHTGACMNPSAPGALAAVS g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c

3120 EF-1 α promoter EF-1 α intron A FRVPTTELQGAQEPAQGRAA a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g

PasI 3180 EF-1 α promoter EF-1 α intron A TYRGARPPLAPGTPVLQTLP g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t

3240 EF-1 α promoter EF-1 α intron A FIAAERGQQLSSLISSAASP a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c g g g t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g c c c

3300 EF-1 α promoter EF-1 α intron A LAPPHTVWVFSFPRETRLRR a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c g c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a g c g

PaeR7IXhoI 3360 EF-1 α promoter EF-1 α intron A KM t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g c t t a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c g a a

3420 EF-1 α promoter EF-1 α intron A t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g

3480 EF-1 α promoter EF-1 α intron A t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a

3540 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a

EcoRINotI 3600 1 5 MEDAK EF-1 α promoter luciferase EF-1 α intron A MEDAK t t t t c t t c c a t t t c a g g t g t c g t g a g c g g c c g c t g a a c t g a a t t c a t g g a a g a t g c c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c g c c g g c g a c t t g a c t t a a g t a c c t t c t a c g g t t t

MreI 3660 10 15 20 25 NIKKGPAPFYPLEDGTAGEQ luciferase NIKKGPAPFYPLEDGTAGEQ a a c a t t a a g a a g g g c c c a g c g c c a t t c t a c c c a c t c g a a g a c g g g a c c g c c g g c g a g c a g t t g t a a t t c t t c c c g g g t c g c g g t a a g a t g g g t g a g c t t c t g c c c t g g c g g c c g c t c g t c

3720 30 35 40 45 LHKAMKRYALVPGTIAFTDA luciferase LHKAMKRYALVPGTIAFTDA c t g c a c a a a g c c a t g a a g c g c t a c g c c c t g g t g c c c g g c a c c a t c g c c t t t a c c g a c g c a g a c g t g t t t c g g t a c t t c g c g a t g c g g g a c c a c g g g c c g t g g t a g c g g a a a t g g c t g c g t

3780 50 55 60 65 HIEVDITYAEYFEMSVRLAE luciferase HIEVDITYAEYFEMSVRLAE c a t a t c g a g g t g g a c a t t a c c t a c g c c g a g t a c t t c g a g a t g a g c g t t c g g c t g g c a g a a g t a t a g c t c c a c c t g t a a t g g a t g c g g c t c a t g a a g c t c t a c t c g c a a g c c g a c c g t c t t

3840 70 75 80 85 AMKRYGLNTNHRIVVCSENS luciferase AMKRYGLNTNHRIVVCSENS g c t a t g a a g c g c t a t g g g c t g a a t a c a a a c c a t c g g a t c g t g g t g t g c a g c g a g a a t a g c c g a t a c t t c g c g a t a c c c g a c t t a t g t t t g g t a g c c t a g c a c c a c a c g t c g c t c t t a t c g

3900 90 95 100 105 LQFFMPVLGALFIGVAVAPA luciferase LQFFMPVLGALFIGVAVAPA t t g c a g t t c t t c a t g c c c g t g t t g g g t g c c c t g t t c a t c g g t g t g g c t g t g g c c c c a g c t a a c g t c a a g a a g t a c g g g c a c a a c c c a c g g g a c a a g t a g c c a c a c c g a c a c c g g g g t c g a

3960 110 115 120 125 NDIYNERELLNSMGISQPTV luciferase NDIYNERELLNSMGISQPTV a a c g a c a t c t a c a a c g a g c g c g a g c t g c t g a a c a g c a t g g g c a t c a g c c a g c c c a c c g t c t t g c t g t a g a t g t t g c t c g c g c t c g a c g a c t t g t c g t a c c c g t a g t c g g t c g g g t g g c a g

4020 130 135 140 145 VFVSKKGLQKILNVQKKLPI luciferase VFVSKKGLQKILNVQKKLPI g t a t t c g t g a g c a a g a a a g g g c t g c a a a a g a t c c t c a a c g t g c a a a a g a a g c t a c c g a t c c a t a a g c a c t c g t t c t t t c c c g a c g t t t t c t a g g a g t t g c a c g t t t t c t t c g a t g g c t a g

BsaBI* 4080 150 155 160 165 IQKIIIMDSKTDYQGFQSMY luciferase IQKIIIMDSKTDYQGFQSMY a t a c a a a a g a t c a t c a t c a t g g a t a g c a a g a c c g a c t a c c a g g g c t t c c a a a g c a t g t a c t a t g t t t t c t a g t a g t a g t a c c t a t c g t t c t g g c t g a t g g t c c c g a a g g t t t c g t a c a t g

4140 170 175 180 185 TFVTSHLPPGFNEYDFVPES luciferase TFVTSHLPPGFNEYDFVPES a c c t t c g t g a c t t c c c a t t t g c c a c c c g g c t t c a a c g a g t a c g a c t t c g t g c c c g a g a g c t g g a a g c a c t g a a g g g t a a a c g g t g g g c c g a a g t t g c t c a t g c t g a a g c a c g g g c t c t c g

4200 190 195 200 205 FDRDKTIALIMNSSGSTGLP luciferase FDRDKTIALIMNSSGSTGLP t t c g a c c g g g a c a a a a c c a t c g c c c t g a t c a t g a a c a g t a g t g g c a g t a c c g g a t t g c c c a a g c t g g c c c t g t t t t g g t a g c g g g a c t a g t a c t t g t c a t c a c c g t c a t g g c c t a a c g g g

4260 210 215 220 225 KGVALPHRTACVRFSHARDP luciferase KGVALPHRTACVRFSHARDP a a g g g c g t a g c c c t a c c g c a c c g c a c c g c t t g t g t c c g a t t c a g t c a t g c c c g c g a c c c c t t c c c g c a t c g g g a t g g c g t g g c g t g g c g a a c a c a g g c t a a g t c a g t a c g g g c g c t g g g g

4320 230 235 240 245 IFGNQIIPDTAILSVVPFHH luciferase IFGNQIIPDTAILSVVPFHH a t c t t c g g c a a c c a g a t c a t c c c c g a c a c c g c t a t c c t c a g c g t g g t g c c a t t t c a c c a c t a g a a g c c g t t g g t c t a g t a g g g g c t g t g g c g a t a g g a g t c g c a c c a c g g t a a a g t g g t g

4380 250 255 260 265 GFGMFTTLGYLICGFRVVLM luciferase GFGMFTTLGYLICGFRVVLM g g c t t c g g c a t g t t c a c c a c g c t g g g c t a c t t g a t c t g c g g c t t t c g g g t c g t g c t c a t g c c g a a g c c g t a c a a g t g g t g c g a c c c g a t g a a c t a g a c g c c g a a a g c c c a g c a c g a g t a c

4440 270 275 280 285 YRFEEELFLRSLQDYKIQSA luciferase YRFEEELFLRSLQDYKIQSA t a c c g c t t c g a g g a g g a g c t a t t c t t g c g c a g c t t g c a a g a c t a t a a g a t t c a a t c t g c c a t g g c g a a g c t c c t c c t c g a t a a g a a c g c g t c g a a c g t t c t g a t a t t c t a a g t t a g a c g g

4500 290 295 300 305 LLVPTLFSFFAKSTLIDKYD luciferase LLVPTLFSFFAKSTLIDKYD c t g c t g g t g c c c a c a c t a t t t a g c t t c t t c g c t a a g a g c a c t c t c a t c g a c a a g t a c g a c g a c g a c c a c g g g t g t g a t a a a t c g a a g a a g c g a t t c t c g t g a g a g t a g c t g t t c a t g c t g

BlpI 4560 310 315 320 325 LSNLHEIASGGAPLSKEVGE luciferase LSNLHEIASGGAPLSKEVGE c t a a g c a a c t t g c a c g a g a t c g c c a g c g g c g g g g c g c c g c t c a g c a a g g a g g t a g g t g a g g a t t c g t t g a a c g t g c t c t a g c g g t c g c c g c c c c g c g g c g a g t c g t t c c t c c a t c c a c t c

4620 330 335 340 345 AVAKRFHLPGIRQGYGLTET luciferase AVAKRFHLPGIRQGYGLTET g c c g t g g c c a a a c g c t t c c a c c t a c c a g g c a t c c g c c a g g g c t a c g g c c t g a c a g a a a c a c g g c a c c g g t t t g c g a a g g t g g a t g g t c c g t a g g c g g t c c c g a t g c c g g a c t g t c t t t g t

4680 350 355 360 365 TSAILITPEGDDKPGAVGKV luciferase TSAILITPEGDDKPGAVGKV a c c a g c g c c a t t c t g a t c a c c c c c g a a g g g g a c g a c a a g c c t g g c g c a g t a g g c a a g g t g t g g t c g c g g t a a g a c t a g t g g g g g c t t c c c c t g c t g t t c g g a c c g c g t c a t c c g t t c c a c

4740 370 375 380 385 VPFFEAKVVDLDTGKTLGVN luciferase VPFFEAKVVDLDTGKTLGVN g t g c c c t t c t t c g a g g c t a a g g t g g t g g a c t t g g a c a c c g g t a a g a c a c t g g g t g t g a a c c a c g g g a a g a a g c t c c g a t t c c a c c a c c t g a a c c t g t g g c c a t t c t g t g a c c c a c a c t t g

HpaI 4800 390 395 400 405 QRGELCVRGPMIMSGYVNNP luciferase QRGELCVRGPMIMSGYVNNP c a g c g c g g c g a g c t g t g c g t c c g t g g c c c c a t g a t c a t g a g c g g c t a c g t t a a c a a c c c c g t c g c g c c g c t c g a c a c g c a g g c a c c g g g g t a c t a g t a c t c g c c g a t g c a a t t g t t g g g g

4860 410 415 420 425 EATNALIDKDGWLHSGDIAY luciferase EATNALIDKDGWLHSGDIAY g a g g c t a c a a a c g c t c t c a t c g a c a a g g a c g g c t g g c t g c a c a g c g g c g a c a t c g c c t a c c t c c g a t g t t t g c g a g a g t a g c t g t t c c t g c c g a c c g a c g t g t c g c c g c t g t a g c g g a t g

4920 430 435 440 445 WDEDEHFFIVDRLKSLIKYK luciferase WDEDEHFFIVDRLKSLIKYK t g g g a c g a g g a c g a g c a c t t c t t c a t c g t g g a c c g g c t g a a g a g c c t g a t c a a a t a c a a g a c c c t g c t c c t g c t c g t g a a g a a g t a g c a c c t g g c c g a c t t c t c g g a c t a g t t t a t g t t c

SexAI*CsiI 4980 450 455 460 465 GYQVAPAELESILLQHPNIF luciferase GYQVAPAELESILLQHPNIF g g c t a c c a g g t a g c c c c a g c c g a a c t g g a g a g c a t c c t g c t g c a a c a c c c c a a c a t c t t c c c g a t g g t c c a t c g g g g t c g g c t t g a c c t c t c g t a g g a c g a c g t t g t g g g g t t g t a g a a g

5040 470 475 480 485 DAGVAGLPDDDAGELPAAVV luciferase DAGVAGLPDDDAGELPAAVV g a c g c c g g g g t c g c c g g c c t g c c c g a c g a c g a t g c c g g c g a g c t g c c c g c c g c a g t c g t c c t g c g g c c c c a g c g g c c g g a c g g g c t g c t g c t a c g g c c g c t c g a c g g g c g g c g t c a g c a g

5100 490 495 500 505 VLEHGKTMTEKEIVDYVASQ luciferase VLEHGKTMTEKEIVDYVASQ g t g c t g g a a c a c g g t a a a a c c a t g a c c g a g a a g g a g a t c g t g g a c t a t g t g g c c a g c c a g c a c g a c c t t g t g c c a t t t t g g t a c t g g c t c t t c c t c t a g c a c c t g a t a c a c c g g t c g g t c

5160 510 515 520 525 VTTAKKLRGGVVFVDEVPKG luciferase VTTAKKLRGGVVFVDEVPKG g t t a c a a c c g c c a a g a a g c t g c g c g g t g g t g t t g t g t t c g t g g a c g a g g t g c c t a a a g g a c a a t g t t g g c g g t t c t t c g a c g c g c c a c c a c a a c a c a a g c a c c t g c t c c a c g g a t t t c c t

5220 530 535 540 545 LTGKLDARKIREILIKAKKG luciferase LTGKLDARKIREILIKAKKG c t g a c c g g c a a g t t g g a c g c c c g c a a g a t c c g c g a g a t t c t c a t t a a g g c c a a g a a g g g c g a c t g g c c g t t c a a c c t g c g g g c g t t c t a g g c g c t c t a a g a g t a a t t c c g g t t c t t c c c g

BamHITspMIXmaISmaIXbaI 5280 550 GKIAV* luciferase IRES2 GKIAV* g g c a a g a t c g c c g t g t a a t a a t t c t a g a g t a c c c g g g c t a g g a t c c g c c c c t c t c c c t c c c c g t t c t a g c g g c a c a t t a t t a a g a t c t c a t g g g c c c g a t c c t a g g c g g g g a g a g g g a g g

5340 IRES2 c c c c c c c c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c c g g t g t g c g t t t g t c t a t g g g g g g g g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g g c c a c a c g c a a a c a g a t a

5400 IRES2 a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c c g g a a a c c t g g c c c t t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c c g g g c c t t t g g a c c g g g a

AvrII 5460 IRES2 G A G A G C G G T T T C C T T A C G IRES reverse g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c a a a g g a a t g c a a g g t c t g c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g t t t c c t t a c g t t c c a g a c

5520 IRES2 t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a a g a c a a a c a a c g t c t g t a a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t t c t g t t t g t t g c a g a c a t

5580 IRES2 g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t g c c t c t g c g g c c a a a a g c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c a c g g a g a c g c c g g t t t t c

PaqCIPmlI 5640 IRES2 c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g t g c c a c g t t g t g a g t t g g g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c a c g g t g c a a c a c t c a a c c

5700 IRES2 T G G C T C T C C T C A A G C G T A T T IRES-F a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a a c a a g g g g c t g a a g g a t t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g t t g t t c c c c g a c t t c c t a

PflMI 5760 IRES2 g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t c g g t a c a c a t g c t t t a c a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a g c c a t g t g t a c g a a a t g t

5820 IRES2 t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c c a c g g g g a c g t g g t t t t c a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g g t g c c c c t g c a c c a a a a g

BstXI 5880 ATG 1 5 MAQSKHGLT IRES2 ZsGreen1 MATTMAQSKHGLT c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a c c a t g g c c c a g t c c a a g c a c g g c c t g a c g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t g g t a c c g g g t c a g g t t c g t g c c g g a c t g

5940 10 15 20 25 KEMTMKYRMEGCVDGHKFVI ZsGreen1 KEMTMKYRMEGCVDGHKFVI c a a g g a g a t g a c c a t g a a g t a c c g c a t g g a g g g c t g c g t g g a c g g c c a c a a g t t c g t g a t g t t c c t c t a c t g g t a c t t c a t g g c g t a c c t c c c g a c g c a c c t g c c g g t g t t c a a g c a c t a

6000 30 35 40 45 TGEGIGYPFKGKQAINLCVV ZsGreen1 TGEGIGYPFKGKQAINLCVV c a c c g g c g a g g g c a t c g g c t a c c c c t t c a a g g g c a a g c a g g c c a t c a a c c t g t g c g t g g t g t g g c c g c t c c c g t a g c c g a t g g g g a a g t t c c c g t t c g t c c g g t a g t t g g a c a c g c a c c a

6060 50 55 60 65 EGGPLPFAEDILSAAFMYGN ZsGreen1 EGGPLPFAEDILSAAFMYGN g g a g g g c g g c c c c t t g c c c t t c g c c g a g g a c a t c t t g t c c g c c g c c t t c a t g t a c g g c a a c c t c c c g c c g g g g a a c g g g a a g c g g c t c c t g t a g a a c a g g c g g c g g a a g t a c a t g c c g t t

Tth111IPflFI 6120 70 75 80 85 RVFTEYPQDIVDYFKNSCPA ZsGreen1 RVFTEYPQDIVDYFKNSCPA c c g c g t g t t c a c c g a g t a c c c c c a g g a c a t c g t c g a c t a c t t c a a g a a c t c c t g c c c c g c g g c g c a c a a g t g g c t c a t g g g g g t c c t g t a g c a g c t g a t g a a g t t c t t g a g g a c g g g g c g

6180 90 95 100 105 GYTWDRSFLFEDGAVCICNA ZsGreen1 GYTWDRSFLFEDGAVCICNA c g g c t a c a c c t g g g a c c g c t c c t t c c t g t t c g a g g a c g g c g c c g t g t g c a t c t g c a a c g c g c c g a t g t g g a c c c t g g c g a g g a a g g a c a a g c t c c t g c c g c g g c a c a c g t a g a c g t t g c g

6240 110 115 120 125 DITVSVEENCMYHESKFYGV ZsGreen1 DITVSVEENCMYHESKFYGV c g a c a t c a c c g t g a g c g t g g a g g a g a a c t g c a t g t a c c a c g a g t c c a a g t t c t a c g g c g t g c t g t a g t g g c a c t c g c a c c t c c t c t t g a c g t a c a t g g t g c t c a g g t t c a a g a t g c c g c a

6300 130 135 140 145 NFPADGPVMKKMTDNWEPSC ZsGreen1 NFPADGPVMKKMTDNWEPSC g a a c t t c c c c g c c g a c g g c c c c g t g a t g a a g a a g a t g a c c g a c a a c t g g g a g c c c t c c t g c t t g a a g g g g c g g c t g c c g g g g c a c t a c t t c t t c t a c t g g c t g t t g a c c c t c g g g a g g a c

6360 150 155 160 165 EKIIPVPKQGILKGDVSMYL ZsGreen1 EKIIPVPKQGILKGDVSMYL c g a g a a g a t c a t c c c c g t g c c c a a g c a g g g c a t c t t g a a g g g c g a c g t g a g c a t g t a c c t g c t c t t c t a g t a g g g g c a c g g g t t c g t c c c g t a g a a c t t c c c g c t g c a c t c g t a c a t g g a

6420 170 175 180 185 LLKDGGRLRCQFDTVYKAKS ZsGreen1 LLKDGGRLRCQFDTVYKAKS g c t g c t g a a g g a c g g t g g c c g c t t g c g c t g c c a g t t c g a c a c c g t g t a c a a g g c c a a g t c c g a c g a c t t c c t g c c a c c g g c g a a c g c g a c g g t c a a g c t g t g g c a c a t g t t c c g g t t c a g

6480 190 195 200 205 VPRKMPDWHFIQHKLTREDR ZsGreen1 VPRKMPDWHFIQHKLTREDR c g t g c c c c g c a a g a t g c c c g a c t g g c a c t t c a t c c a g c a c a a g c t g a c c c g c g a g g a c c g g c a c g g g g c g t t c t a c g g g c t g a c c g t g a a g t a g g t c g t g t t c g a c t g g g c g c t c c t g g c

6540 210 215 220 225 SDAKNQKWHLTEHAIASGSA ZsGreen1 SDAKNQKWHLTEHAIASGSA c a g c g a c g c c a a g a a c c a g a a g t g g c a c c t g a c c g a g c a c g c c a t c g c c t c c g g c t c c g c g t c g c t g c g g t t c t t g g t c t t c a c c g t g g a c t g g c t c g t g c g g t a g c g g a g g c c g a g g c g

BspDIClaI 6600 230 LP* ZsGreen1 KS primer C T A T G G C A G C T G G A G C T pBluescriptKS LP* c t t g c c c t a a g g a t c g g c c g c a t c g a t a c c g t c g a c c t c g a t c g a g a c c t a g a a a a a c a t g a a c g g g a t t c c t a g c c g g c g t a g c t a t g g c a g c t g g a g c t a g c t c t g g a t c t t t t t g t a

6660 g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g a a g c a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c t t c g t

6720 c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t g a c t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t a c t g a

6780 t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t

6840 a t t c a c t c c c a a c g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a c a c a a g g c t a c t a a g t g a g g g t t g c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t g t g t t c c g a t g

6900 t t c c c t g a t t g g c a g a a c t a c a c a c c a g g g c c a g g g a t c a g a t a t c c a c t g a c c t t t g g a a a g g g a c t a a c c g t c t t g a t g t g t g g t c c c g g t c c c t a g t c t a t a g g t g a c t g g a a a c c t

6960 t g g t g c t a c a a g c t a g t a c c a g t t g a g c a a g a g a a g g t a g a a g a a g c c a a t g a a g g a g a g a c c a c g a t g t t c g a t c a t g g t c a a c t c g t t c t c t t c c a t c t t c t t c g g t t a c t t c c t c t c

7020 a a c a c c c g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g a g a g a g a a g t a t t g t g g g c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c t c t c t c t t c a t

BspEI 7080 t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c a t g g c c c g a g a g c t g c a t c c g a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g t a c c g g g c t c t c g a c g t a g g c

7140 5' LTR (truncated) g a c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a c t g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t

7200 5' LTR (truncated) g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g

7260 5' LTR (truncated) c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t

7320 5' LTR (truncated) a t c t c t a g c a g c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t a g a g a t c g t c g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c

7380 ori t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t

7440 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g

7500 ori t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g

7560 ori c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c

7620 ori g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g

7680 ori t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t

7740 ori g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c

7800 ori a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c

7860 ori a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a

7920 ori g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t

7980 g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t

8040 g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t

8100 285 *WH AmpR *WH t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g

8160 280 275 270 265 KILSAGIEAIQRNREDMTAQ AmpR KILSAGIEAIQRNREDMTAQ t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t

8220 260 255 250 245 SGTTYIVVIRSPKGDPGLAA AmpR SGTTYIVVIRSPKGDPGLAA c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t

8280 240 235 230 225 IIGRSGREGAGSKDAIFWGA AmpR IIGRSGREGAGSKDAIFWGA a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g

8340 220 215 210 205 PLASRLLPGAVKDAEMWDIL AmpR PLASRLLPGAVKDAEMWDIL g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a

8400 200 195 190 185 QQRSALTLLEGTLLKRLTTA AmpR QQRSALTLLEGTLLKRLTTA t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g

8460 180 175 170 165 MAVPMTTDREDNPIAENLEP AmpR MAVPMTTDREDNPIAENLEP a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a

8520 160 155 150 145 EWRDLRTVHDGMNHLFATLE AmpR EWRDLRTVHDGMNHLFATLE t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g

8580 140 135 130 125 KPGGITTLLLNAATNDSMTI AmpR KPGGITTLLLNAATNDSMTI t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c

8640 120 115 110 105 AASCLERVTMGDTLHKETVP AmpR AASCLERVTMGDTLHKETVP g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a

8700 100 95 90 85 SYEVLDNQSYHIRRGLQEQG AmpR SYEVLDNQSYHIRRGLQEQG g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c

8760 80 75 70 65 ADIRSLVAGCLLVKFTSMMP AmpR A T A A T A C C G C G C C A C A T A G C Amp-R ADIRSLVAGCLLVKFTSMMP g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t

8820 60 55 50 45 FREEPRFSELIKGSNLDLEI AmpR FREEPRFSELIKGSNLDLEI a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c

8880 40 35 30 25 YGVRAGLQDEADKVKVLTEP AmpR YGVRAGLQDEADKVKVLTEP t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c

8940 H 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFH AmpR HAFVPLCFAAFFPILAVRFH t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a

SspI 9000 1 signal sequence QISM AmpR AmpR promoter QISM t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g

9060 AmpR promoter a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t

SgrDI 9120 t t t c c c c g a a a a g t g c c a c c t g a c g t c g a c g g a t c g g g a g a t c t c c c g a t c c c c t a t g g t a a a g g g g c t t t t c a c g g t g g a c t g c a g c t g c c t a g c c c t c t a g a g g g c t a g g g g a t a c c a

9180 A T G T T A G A C G A G A C T A C G G C pRS-marker g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a

9240 g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a

9300 t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c

MluI 9321 t a c g g g c c a g a t a t a c g c g t t a t g c c c g g t c t a t a t g c g c a a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.