Addgene PROM_Ntpsba_chlo - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: PROM_Ntpsba_chlo


Map View

ScaI (2055) TatI (2053) EcoRV (2020) AcuI (1628) AlwNI (1496) ApaLI (1394) PspFI (1388) BseYI (1384) HaeII (1328) BciVI (1283) pBR322ori-F (1221 .. 1240) NspI (1084) SspI (38) BtgI - NcoI - StyI (45) MscI (83) PasI (115) PflMI * (121) BsmBI - Esp3I (122) AclI (261) BsrDI (333) BspEI (350) PvuII (450) TsoI (451) CAT-R (479 .. 498) MslI (634) BsaAI (657) BsaHI (685) ZraI (686) AatII (688) pBRforEco (726 .. 744) EcoRI (794) HincII (847) PstI (887) L4440 (970 .. 987) AflIII - PciI (1080) PROM_Ntpsba_chlo 2122 bp

Sequence View

NcoIStyIBtgISspI 5ʹ 3ʹ 60 185 180 175 170 ALPMLVKDGQTYYKGMTFVP CmR ALPMLVKDGQTYYKGMTFVP c g c c a g c g g c a t c a g c a c c t t g t c g c c t t g c g t a t a a t a t t t g c c c a t g g t g a a a a c g g g g c g g t c g c c g t a g t c g t g g a a c a g c g g a a c g c a t a t t a t a a a c g g g t a c c a c t t t t g c c c

PasIMscI 120 165 160 155 150 AFFNDMNAVNLDFSTFSVWP CmR AFFNDMNAVNLDFSTFSVWP g g c g a a g a a g t t g t c c a t a t t g g c c a c g t t t a a a t c a a a a c t g g t g a a a c t c a c c c a g g g c c g c t t c t t c a a c a g g t a t a a c c g g t g c a a a t t t a g t t t t g a c c a c t t t g a g t g g g t c c c

PflMI*BsmBIEsp3I 180 145 140 135 130 NASVFFMNEIFGKPFYALNE CmR NASVFFMNEIFGKPFYALNE a t t g g c t g a g a c g a a a a a c a t a t t c t c a a t a a a c c c t t t a g g g a a a t a g g c c a g g t t t t c t a a c c g a c t c t g c t t t t t g t a t a a g a g t t a t t t g g g a a a t c c c t t t a t c c g g t c c a a a a g

240 125 120 115 110 GYCAVDQSYIHLFQRFDDHY CmR GYCAVDQSYIHLFQRFDDHY a c c g t a a c a c g c c a c a t c t t g c g a a t a t a t g t g t a g a a a c t g c c g g a a a t c g t c g t g g t a t g g c a t t g t g c g g t g t a g a a c g c t t a t a t a c a c a t c t t t g a c g g c c t t t a g c a g c a c c a t

AclI 300 105 100 95 90 ESWLSSFTETQEHFVTYCPH CmR ESWLSSFTETQEHFVTYCPH t t c a c t c c a g a g c g a t g a a a a c g t t t c a g t t t g c t c a t g g a a a a c g g t g t a a c a a g g g t g a a g t g a g g t c t c g c t a c t t t t g c a a a g t c a a a c g a g t a c c t t t t g c c a c a t t g t t c c c a c

BspEIBsrDI 360 85 80 75 70 VSDWIVLEGDKMAMRFEPHA CmR VSDWIVLEGDKMAMRFEPHA a a c a c t a t c c c a t a t c a c c a g c t c a c c g t c t t t c a t t g c c a t a c g a a a t t c c g g a t g a g c t t g t g a t a g g g t a t a g t g g t c g a g t g g c a g a a a g t a a c g g t a t g c t t t a a g g c c t a c t c g

420 65 60 55 50 NMLRALIHIFAPYFKHKNKK CmR NMLRALIHIFAPYFKHKNKK a t t c a t c a g g c g g g c a a g a a t g t g a a t a a a g g c c g g a t a a a a c t t g t g c t t a t t t t t c t t t a a g t a g t c c g c c c g t t c t t a c a c t t a t t t c c g g c c t a t t t t g a a c a c g a a t a a a a a g a a

PvuIITsoI 480 45 40 35 30 VTKLFATIDLQVTQNYTCQA CmR G C CAT-R VTKLFATIDLQVTQNYTCQA t a c g g t c t t t a a a a a g g c c g t a a t a t c c a g c t g a a c g g t c t g g t t a t a g g t a c a t t g a g c a t g c c a g a a a t t t t t c c g g c a t t a t a g g t c g a c t t g c c a g a c c a a t a t c c a t g t a a c t c g

540 25 20 15 10 VSQFAEFHEKRHWQSIDVTT CmR A A C T G A C T G A A A T G C C T C CAT-R VSQFAEFHEKRHWQSIDVTT a a c t g a c t g a a a t g c c t c a a a a t g t t c t t t a c g a t g c c a t t g g g a t a t a t c a a c g g t g g t t t g a c t g a c t t t a c g g a g t t t t a c a a g a a a t g c t a c g g t a a c c c t a t a t a g t t g c c a c c a

600 5 1 YGTIKKEM CmR YGTIKKEM a t a t c c a g t g a t t t t t t t c t c c a t t t t a g c t t c c t t a g c t c c t g a a a a t c t c g a t a a c t c t a t a g g t c a c t a a a a a a a g a g g t a a a a t c g a a g g a a t c g a g g a c t t t t a g a g c t a t t g a g

BsaAIMslI 660 a a a a a a t a c g c c c g g t a g t g a t c t t a t t t c a t t a t g g t g a a a g t t g g a a c c t c t t a c g t g t t t t t t a t g c g g g c c a t c a c t a g a a t a a a g t a a t a c c a c t t t c a a c c t t g g a g a a t g c a c

BsaHIZraIAatII 720 c c c g a t c a a c t c g a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t g g g c t a g t t g a g c t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a

780 bacterial terminator A A T A G G C G T A T C A C G A G G C pBRforEco a t a a a a a t a g g c g t a t c a c g a g g c a g a a t t t c a g a t a a a a a a a a t c c t t a g c t t t c g c t a t a t t t t t a t c c g c a t a g t g c t c c g t c t t a a a g t c t a t t t t t t t t a g g a a t c g a a a g c g a t

EcoRI 840 bacterial terminator a g g a t g a t t t c t g g a a t t c g g t c t c t a g t a t t a t t c a a c a g t a t a a c a t g a c t t a t a t a c t c c t a c t a a a g a c c t t a a g c c a g a g a t c a t a a t a a g t t g t c a t a t t g t a c t g a a t a t a t g

PstIHincII 900 his operon terminator t c g t g t c a a c c a a g a g c t c g a a t t g g c c g c t c t c c a g a g a c c c t g c a g t c c g g c a a a a a a a g c a c a g t t g g t t c t c g a g c t t a a c c g g c g a g a g g t c t c t g g g a c g t c a g g c c g t t t t t t

960 his operon terminator g g g c a a g g t g t c a c c a c c c t g c c c t t t t t c t t t a a a a c c g a a a a g a t t a c t t c g c g t t a t c c c g t t c c a c a g t g g t g g g a c g g g a a a a a g a a a t t t t g g c t t t t c t a a t g a a g c g c a a t a

1020 G A G C G A G T G A C T G A G C G A L4440 g c a g g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c c g t c c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g

1080 a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t

AflIIIPciINspI 1140 c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a

1200 ori t t t c c a c a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g a a a g g t g t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c

1260 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c

BciVI 1320 ori c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c

HaeII 1380 ori c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g

ApaLIBseYIPspFI 1440 ori c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t

AlwNI 1500 ori c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c

1560 ori t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g

1620 ori t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g

AcuI 1680 ori c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c

1740 ori t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a

1800 g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a

1860 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t

1920 lambda t0 terminator a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g c t c g a g g c t t g g a t t c t c a c c t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c g a g c t c c g a a c c t a a g a g t g g

1980 lambda t0 terminator a a t a a a a a a c g c c c g g c g g c a a c c g a g c g t t c t g a a c a a a t c c a g a t g g a g t t c t g a g g t t t a t t t t t t g c g g g c c g c c g t t g g c t c g c a a g a c t t g t t t a g g t c t a c c t c a a g a c t c c a

EcoRV 2040 220 *AGGQ lambda t0 terminator CmR *AGGQ c a t t a c t g g a t c t a t c a a c a g g a g t c c a a g c g a g c t c g a t a t c a a a t t a c g c c c c g c c c t g t a a t g a c c t a g a t a g t t g t c c t c a g g t t c g c t c g a g c t a t a g t t t a a t g c g g g g c g g g a

TatIScaI 2100 215 210 205 200 WEDCYQQLENLMRGVHFGDC CmR WEDCYQQLENLMRGVHFGDC g c c a c t c a t c g c a g t a c t g t t g t a a t t c a t t a a g c a t t c t g c c g a c a t g g a a g c c a t c a c c g g t g a g t a g c g t c a t g a c a a c a t t a a g t a a t t c g t a a g a c g g c t g t a c c t t c g g t a g t g

3ʹ 5ʹ 2122 195 190 VAHHVQI CmR VAHHVQI a a a c g g c a t g a t g a a c c t g a a t t t t g c c g t a c t a c t t g g a c t t a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.