Addgene pFA6a-link-ymNeongreen-URA3 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pFA6a-link-ymNeongreen-URA3 Sequencing Result


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PfoI (18) pBRforEco (4859 .. 4877) XmnI (4498) Amp-R (4449 .. 4468) PvuI (4269) NmeAIII (4047) AlwNI (3422) PspFI (3314) BseYI (3310) pBR322ori-F (3147 .. 3166) PciI (3006) L4440 (2896 .. 2913) BspQI - SapI (2890) HpaI (2827) T7 (2790 .. 2809) SacII (2775) SfiI (2768) SpeI (2755) EcoRV (2745) BspDI - ClaI (2738) BanII - SacI (2729) Eco53kI (2727) MscI (2470) pGEX 3' (1 .. 23) pRS-marker (123 .. 142) SP6 (223 .. 240) PvuII (254) HindIII (258) BsiWI (264) PstI (274) PacI (306) NcoI (570) BstBI (615) PshAI (753) BsrGI (1011) AbsI - AvaI - BsoBI - PaeR7I - PspXI - XhoI (1030) AscI - BssHII (1043) BstZ17I (1121) pHybLex-R (1117 .. 1141) ADH1 terminator I-SceI (1267) BglII (1279) BmgBI (1362) Bpu10I (1371) BseRI (1473) MluI (1526) MfeI (1777) PpuMI (1827) BsaAI (1917) pFA6a-link-ymNeongreen-URA3 4914 bp

Sequence View

PfoI 60 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c

120 c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t c g g g g c t g g c t t a a c t a g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a g c c c c g a c c g a a t t g a t

180 C G G C A T C A G A G C A G A T T G T A pRS-marker t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t a t g g a c a t a t t g t c g t t a g a a c g a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a t a c c t g t a t a a c a g c a a t c t t g c

240 SP6 promoter A T T T A G G T G A C A C T A T A G SP6 c g g c t a c a a t t a a t a c a t a a c c t t a t g t a t c a t a c a c a t a c g a t t t a g g t g a c a c t a t a g g c c g a t g t t a a t t a t g t a t t g g a a t a c a t a g t a t g t g t a t g c t a a a t c c a c t g t g a t a t c

PstIBsiWIPvuIIHindIII 300 SP6 promoter a a c g c g g c c g c c a g c t g a a g c t t c g t a c g c t g c a g g t c g a c g g a t c g g t g a c g g t g c t g g t t g c g c c g g c g g t c g a c t t c g a a g c a t g c g a c g t c c a g c t g c c t a g c c a c t g c c a c g a c c

PacI 360 MVSKGEEDNMASLPATH *NVHDRLTFFIVHSRQWGGV t t t a a t t a a c a t g g t c t c t a a g g g t g a a g a a g a t a a c a t g g c t t c t t t g c c a g c c a c c c a a a a t t a a t t g t a c c a g a g a t t c c c a c t t c t t c t a t t g t a c c g a a g a a a c g g t c g g t g g g t

420 ELHIFGSINGVDFDMVGQGT LQVDETGNVTDVEIHDTLTG c g a g t t g c a c a t c t t c g g t t c c a t t a a c g g t g t c g a c t t c g a t a t g g t c g g t c a a g g t a c g c t c a a c g t g t a g a a g c c a a g g t a a t t g c c a c a g c t g a a g c t a t a c c a g c c a g t t c c a t g

480 GNPNDGYEELNLKSTKGDLQ TVWVVTVFFQVQLRGLAVQL c g g t a a c c c a a a c g a c g g t t a c g a a g a a t t g a a c t t g a a g t c t a c c a a g g g c g a c t t g c a g c c a t t g g g t t t g c t g c c a a t g c t t c t t a a c t t g a a c t t c a g a t g g t t c c c g c t g a a c g t

540 FSPWILVPHIGYGFHQYLPY EGRPNQNWVDTVTEVLVQWV g t t c t c c c c t t g g a t t t t g g t t c c a c a c a t c g g t t a c g g t t t c c a c c a a t a c t t g c c a t a c a a g a g g g g a a c c t a a a a c c a a g g t g t g t a g c c a a t g c c a a a g g t g g t t a t g a a c g g t a t

NcoI 600 Kozak sequence PDGMSPFQAAMVDGSGYQVH WVTHGWELGGHNVTRTVLDV c c c a g a c g g t a t g t c c c c a t t c c a a g c c g c c a t g g t t g a c g g t t c t g g t t a c c a a g t c c a g g g t c t g c c a t a c a g g g g t a a g g t t c g g c g g t a c c a a c t g c c a a g a c c a a t g g t t c a g g t

BstBI 660 RTMQFEDGASLTVNYRYTYE SGHLEFVTSGQGHVVSVGVF c a g a a c c a t g c a a t t c g a a g a c g g t g c t t c c t t g a c c g t g a a c t a c a g a t a c a c c t a c g a g t c t t g g t a c g t t a a g c t t c t g c c a c g a a g g a a c t g g c a c t t g a t g t c t a t g t g g a t g c t

720 GSHIKGEAQVKGTGFPADGP TRVDLAFGLNLTGTERSVTW a g g t t c t c a c a t c a a g g g c g a a g c c c a a g t t a a g g g t a c c g g t t t c c c t g c t g a c g g t c c t c c a a g a g t g t a g t t c c c g c t t c g g g t t c a a t t c c c a t g g c c a a a g g g a c g a c t g c c a g g

PshAI 780 VMTNSLTAADWCRSKKTYPN DHSVGQGSSVPTTGLLGVWV a g t c a t g a c t a a c t c c t t g a c c g c t g c t g a c t g g t g t c g t t c c a a g a a g a c c t a c c c a a a t c a g t a c t g a t t g a g g a a c t g g c g a c g a c t g a c c a c a g c a a g g t t c t t c t g g a t g g g t t t

840 DKTIISTFKWSYTTGNGKRY ILGDNGSELPGVGGTVALSV c g a t a a g a c c a t c a t t t c c a c t t t c a a g t g g t c c t a c a c c a c c g g t a a c g g c a a g a g a t a g c t a t t c t g g t a g t a a a g g t g a a a g t t c a c c a g g a t g t g g t g g c c a t t g c c g t t c t c t a t

900 RSTARTTYTFAKPMAANYLK ARGSTGGVGEGLWHSGVVQL c c g c t c t a c c g c t c g t a c c a c c t a c a c c t t c g c c a a g c c a a t g g c t g c c a a c t a c t t g a a g g c g a g a t g g c g a g c a t g g t g g a t g t g g a a g c g g t t c g g t t a c c g a c g g t t g a t g a a c t t

960 NQPMYVFRKTELKHSKTELN VLRHVDETLGLQLM g a a c c a g c c t a t g t a c g t c t t c c g t a a g a c c g a g c t g a a g c a t t c c a a g a c t g a a t t g a a c t t g g t c g g a t a c a t g c a g a a g g c a t t c t g g c t c g a c t t c g t a a g g t t c t g a c t t a a c t t

BsrGI 1020 FKEWQKAFTDVMGMDELYK* c t t c a a g g a a t g g c a a a a g g c t t t c a c c g a c g t c a t g g g t a t g g a c g a a t t g t a c a a g t a g a a g t t c c t t a c c g t t t t c c g a a a g t g g c t g c a g t a c c c a t a c c t g c t t a a c a t g t t c a t

BssHIIAscIPaeR7IBsoBIPspXIXhoIAvaIAbsI 1080 ADH1 terminator a g c g g c c g c c t c g a g g t c g a c g g c g c g c c a c t t c t a a a t a a g c g a a t t t c t t a t g a t t t a t c g c c g g c g g a g c t c c a g c t g c c g c g c g g t g a a g a t t t a t t c g c t t a a a g a a t a c t a a a t

BstZ17I 1140 ADH1 terminator C A C A T A T G T T T A A A A T T T C A C T G A pHybLex-R t g a t t t t t a t t a t t a a a t a a g t t a t a a a a a a a a t a a g t g t a t a c a a a t t t t a a a g t g a c t a c t a a a a a t a a t a a t t t a t t c a a t a t t t t t t t t a t t c a c a t a t g t t t a a a a t t t c a c t g a

1200 ADH1 terminator G pHybLex-R c t t a g g t t t t a a a a c g a a a a t t c t t a t t c t t g a g t a a c t c t t t c c t g t a g g t c a g g t t g c g a a t c c a a a a t t t t g c t t t t a a g a a t a a g a a c t c a t t g a g a a a g g a c a t c c a g t c c a a c g

1260 ADH1 terminator t t t c t c a g g t a t a g t a t g a g g t c g c t c t t a t t g a c c a c a c c t c t a c c g g c a g a t c c g c t a a a a g a g t c c a t a t c a t a c t c c a g c g a g a a t a a c t g g t g t g g a g a t g g c c g t c t a g g c g a t

BglIII-SceI 1320 g g g a t a a c a g g g t a a t a t a g a t c t g t t t a g c t t g c c t c g t c c c c g c c g g g t c a c c c g g c c c c c t a t t g t c c c a t t a t a t c t a g a c a a a t c g a a c g g a g c a g g g g c g g c c c a g t g g g c c g g

Bpu10IBmgBI 1380 TEF promoter a g c g a c a t g g a g g c c c a g a a t a c c c t c c t t g a c a g t c t t g a c g t g c g c a g c t c a g g g g c a t c g c t g t a c c t c c g g g t c t t a t g g g a g g a a c t g t c a g a a c t g c a c g c g t c g a g t c c c c g t

1440 TEF promoter t g a t g t g a c t g t c g c c c g t a c a t t t a g c c c a t a c a t c c c c a t g t a t a a t c a t t t g c a t c c a c t a c a c t g a c a g c g g g c a t g t a a a t c g g g t a t g t a g g g g t a c a t a t t a g t a a a c g t a g g

BseRI 1500 TEF promoter a t a c a t t t t g a t g g c c g c a c g g c g c g a a g c a a a a a t t a c g g c t c c t c g c t g c g g a c c t g c t a t g t a a a a c t a c c g g c g t g c c g c g c t t c g t t t t t a a t g c c g a g g a g c g a c g c c t g g a c g

MluI 1560 TEF promoter g a g c a g g g a a a c g c t c c c c t c a c a g a c g c g t t g a a t t g t c c c c a c g c c g c g c c c c t g t a g c t c g t c c c t t t g c g a g g g g a g t g t c t g c g c a a c t t a a c a g g g g t g c g g c g c g g g g a c a t c

1620 TEF promoter a g a a a t a t a a a a g g t t a g g a t t t g c c a c t g a g g t t c t t c t t t c a t a t a c t t c c t t t t a a a t c t t t a t a t t t t c c a a t c c t a a a c g g t g a c t c c a a g a a g a a a g t a t a t g a a g g a a a a t t t

1680 TEF promoter MTVN a t c t t g c t a g g a t a c a g t t c t c a c a t c a c a t c c g a a c a t a a a c a a c c a t g a c a g t c a a c a t a g a a c g a t c c t a t g t c a a g a g t g t a g t g t a g g c t t g t a t t t g t t g g t a c t g t c a g t t g t

1740 TKTYSERAETHASPVAQRLF c t a a g a c c t a t a g t g a g a g a g c a g a a a c t c a t g c c t c a c c a g t a g c a c a a c g a t t a t t t c g a t t c t g g a t a t c a c t c t c t c g t c t t t g a g t a c g g a g t g g t c a t c g t g t t g c t a a t a a a g

MfeI 1800 RLMELKKTNLCASIDVDTTK g a t t a a t g g a a c t g a a g a a a a c c a a t t t a t g t g c a t c a a t t g a t g t t g a t a c c a c t a a g g c t a a t t a c c t t g a c t t c t t t t g g t t a a a t a c a c g t a g t t a a c t a c a a c t a t g g t g a t t c c

PpuMI 1860 EFLELIDKLGPYVCLIKTHI a a t t c c t t g a a t t a a t t g a t a a a t t g g g t c c t t a t g t a t g c t t a a t c a a g a c t c a t a t t g t t a a g g a a c t t a a t t a a c t a t t t a a c c c a g g a a t a c a t a c g a a t t a g t t c t g a g t a t a a c

BsaAI 1920 DIINDFSYESTIEPLLELSR a t a t a a t c a a t g a t t t t t c c t a t g a a t c c a c t a t t g a a c c a t t a t t a g a a c t t t c a c g t a t a t a t t a g t t a c t a a a a a g g a t a c t t a g g t g a t a a c t t g g t a a t a a t c t t g a a a g t g c a t

1980 KHQFMIFEDRKFADIGNTVK a a c a t c a a t t t a t g a t t t t t g a a g a t a g a a a a t t t g c t g a t a t t g g t a a t a c c g t g a a g a t t g t a g t t a a a t a c t a a a a a c t t c t a t c t t t t a a a c g a c t a t a a c c a t t a t g g c a c t t c t

2040 KQYIGGVYKISSWADITNAH a a c a a t a t a t t g g t g g a g t t t a t a a a a t t a g t a g t t g g g c a g a t a t t a c t a a t g c t c a t g t t g t t a t a t a a c c a c c t c a a a t a t t t t a a t c a t c a a c c c g t c t a t a a t g a t t a c g a g t a c

2100 GVTGNGVVEGLKQGAKETTT g t g t c a c t g g g a a t g g a g t a g t t g a a g g a t t a a a a c a g g g a g c t a a a g a a a c c a c c a c c a c a c a g t g a c c c t t a c c t c a t c a a c t t c c t a a t t t t g t c c c t c g a t t t c t t t g g t g g t g g t

2160 NQEPRGLLMLAELSSVGSLA a c c a a g a g c c a a g a g g g t t a t t g a t g t t a g c t g a a t t a t c a t c a g t g g g a t c a t t a g c a t t g g t t c t c g g t t c t c c c a a t a a c t a c a a t c g a c t t a a t a g t a g t c a c c c t a g t a a t c g t a

2220 YGEYSQKTVEIAKSDKEFVI a t g g a g a a t a t t c t c a a a a a a c t g t t g a a a t t g c t a a a t c c g a t a a g g a a t t t g t t a t t g t a c c t c t t a t a a g a g t t t t t t g a c a a c t t t a a c g a t t t a g g c t a t t c c t t a a a c a a t a a c

2280 GFIAQRDMGGQEEGFDWLIM g a t t t a t t g c c c a a c g t g a t a t g g g t g g a c a a g a a g a a g g a t t t g a t t g g c t t a t t a t g a c t a a a t a a c g g g t t g c a c t a t a c c c a c c t g t t c t t c t t c c t a a a c t a a c c g a a t a a t a c t

2340 TPGVGLDDKGDGLGQQYRTV c a c c t g g a g t t g g a t t a g a t g a t a a a g g t g a t g g a t t a g g a c a a c a a t a t a g a a c t g t t g g t g g a c c t c a a c c t a a t c t a c t a t t t c c a c t a c c t a a t c c t g t t g t t a t a t c t t g a c a a c

2400 DEVVSTGTDIIIVGRGLFGK a t g a a g t t g t t a g c a c t g g a a c t g a t a t t a t c a t t g t t g g t a g a g g a t t g t t t g g t a a a g t a c t t c a a c a a t c g t g a c c t t g a c t a t a a t a g t a a c a a c c a t c t c c t a a c a a a c c a t t t c

2460 GRDPDIEGKRYRDAGWNAYL g a a g a g a t c c a g a t a t t g a a g g t a a a a g g t a t a g a g a t g c t g g t t g g a a t g c t t a t t t g a c t t c t c t a g g t c t a t a a c t t c c a t t t t c c a t a t c t c t a c g a c c a a c c t t a c g a a t a a a c t

MscI 2520 TEF terminator KKTGQL* a a a a g a c t g g c c a a t t a t a a t c a g t a c t g a c a a t a a a a a g a t t c t t g t t t t c a a g a a c t t t t t t c t g a c c g g t t a a t a t t a g t c a t g a c t g t t a t t t t t c t a a g a a c a a a a g t t c t t g a a

2580 TEF terminator g t c a t t t g t a t a g t t t t t t t a t a t t g t a g t t g t t c t a t t t t a a t c a a a t g t t a g c g t g a t c a g t a a a c a t a t c a a a a a a a t a t a a c a t c a a c a a g a t a a a a t t a g t t t a c a a t c g c a c t a

2640 TEF terminator t t a t a t t t t t t t t c g c c t c g a c a t c a t c t g c c c a g a t g c g a a g t t a a g t g c g c a g a a a g t a a t a t a a a a a a a a g c g g a g c t g t a g t a g a c g g g t c t a c g c t t c a a t t c a c g c g t c t t t c a

2700 TEF terminator a a t a t c a t g c g t c a a t c g t a t g t g a a t g c t g g t c g c t a t a c t g c t g t c g a t t c g a t a c t a t t a t a g t a c g c a g t t a g c a t a c a c t t a c g a c c a g c g a t a t g a c g a c a g c t a a g c t a t g a t

SpeIEcoRVBspDIClaIEco53kIBanIISacI 2760 a c g c c g c c a t c c a g t g t c g a a a a c g a g c t c g a a t t c a t c g a t g a t a t c a g a t c c a c t a g t t g c g g c g g t a g g t c a c a g c t t t t g c t c g a g c t t a a g t a g c t a c t a t a g t c t a g g t g a t c a

SacIISfiI 2820 T7 promoter G G G A T A T C A C T C A G C A T A A T T7 g g c c t a t g c g g c c g c g g a t c t g c c g g t c t c c c t a t a g t g a g t c g t a t t a a t t t c g a t a a g c c g g a t a c g c c g g c g c c t a g a c g g c c a g a g g g a t a t c a c t c a g c a t a a t t a a a g c t a t t c

HpaI 2880 c c a g g t t a a c c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g g t c c a a t t g g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c

BspQISapI 2940 G A G C G A G T G A C T G A G C G A L4440 g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g

3000 g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c

PciI 3060 a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a

3120 ori g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t

3180 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a

3240 ori c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g

3300 ori g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a

BseYIPspFI 3360 ori t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g

3420 ori c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g

AlwNI 3480 ori c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c

3540 ori g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g

3600 ori a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c

3660 ori c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t

3720 t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a

3780 t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c

3840 285 *WHKI AmpR *WHKI t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a

3900 280 275 270 265 LSAGIEAIQRNREDMTAQSG AmpR LSAGIEAIQRNREDMTAQSG c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g

3960 260 255 250 245 TTYIVVIRSPKGDPGLAAII AmpR TTYIVVIRSPKGDPGLAAII c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a

4020 240 235 230 225 GRSGREGAGSKDAIFWGAPL AmpR GRSGREGAGSKDAIFWGAPL a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c

NmeAIII 4080 220 215 210 205 ASRLLPGAVKDAEMWDILQQ AmpR ASRLLPGAVKDAEMWDILQQ g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c

4140 200 195 190 185 RSALTLLEGTLLKRLTTAMA AmpR RSALTLLEGTLLKRLTTAMA c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g

4200 180 175 170 165 VPMTTDREDNPIAENLEPEW AmpR VPMTTDREDNPIAENLEPEW t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t

4260 160 155 150 145 RDLRTVHDGMNHLFATLEKP AmpR RDLRTVHDGMNHLFATLEKP a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c

PvuI 4320 140 135 130 125 GGITTLLLNAATNDSMTIAA AmpR GGITTLLLNAATNDSMTIAA t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g

4380 120 115 110 105 SCLERVTMGDTLHKETVPSY AmpR SCLERVTMGDTLHKETVPSY a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t

4440 100 95 90 85 EVLDNQSYHIRRGLQEQGAD AmpR EVLDNQSYHIRRGLQEQGAD c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g

XmnI 4500 80 75 70 65 IRSLVAGCLLVKFTSMMPFR AmpR A T A A T A C C G C G C C A C A T A G C Amp-R IRSLVAGCLLVKFTSMMPFR a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c

4560 60 55 50 45 EEPRFSELIKGSNLDLEIYG AmpR EEPRFSELIKGSNLDLEIYG t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g

4620 40 35 30 25 VRAGLQDEADKVKVLTEPH sig... A AmpR VRAGLQDEADKVKVLTEPHA c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g

4680 20 15 10 5 signal sequence FVPLCFAAFFPILAVRFHQI AmpR FVPLCFAAFFPILAVRFHQI a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a

4740 1 signal se... SM AmpR AmpR promoter SM a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c

4800 AmpR promoter c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g

4860 A A pBRforEco c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t a t c a t g a c a t t a a c c t a t a a a a a g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a t a g t a c t g t a a t t g g a t a t t t t t

4914 T A G G C G T A T C A C G A G G C pBRforEco t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t t c g g t g a t g a c g g t g a a a a a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a a a g c c a c t a c t g c c a c t t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.