Addgene miniTol2 x EF1a_EKAREN5(TA) + PGK-blast Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: miniTol2 x EF1a_EKAREN5(TA) + PGK-blast Sequencing Result


Map View

M13 Forward (8111 .. 8128) M13/pUC Forward (8097 .. 8119) F1ori-R (7865 .. 7884) F1ori-F (7653 .. 7674) Amp-R (7096 .. 7115) BsaI (6607) pBR322ori-F (5794 .. 5813) L4440 (5543 .. 5560) BspQI - SapI (5537) CAP binding site lac promoter M13/pUC Reverse (5325 .. 5347) lac operator M13 Reverse (5312 .. 5328) M13 rev SP6 (5277 .. 5294) SP6 promoter NsiI (5263) BstXI (5254) PacI (5021) BGH-rev (4777 .. 4794) NruI * (4562) MscI (4334) NcoI (4329) mPGK-F (4186 .. 4205) BlpI (4027) M13 fwd AflII (57) SwaI (275) BglII (280) FseI (974) BstAPI (1050) BbvCI (1172) EF1a-F (1396 .. 1416) AscI - BssHII (1476) BstBI (1499) EcoRV (1519) EXFP-R (1862 .. 1881) Acc65I (2438) KpnI (2442) BamHI (2701) NotI (2734) Bsu36I (3009) SV40 NLS NheI (3502) BmtI (3506) SnaBI (3552) EBV-rev (3580 .. 3599) SV40pA-R (3634 .. 3653) BspDI - ClaI (3705) MSCV-rev (3789 .. 3812) PGK promoter miniTol2 x EF1a_EKAREN5(TA) + PGK-blast 8151 bp

Sequence View

AflII 60 T7 promoter g g g c g a a t t g g g c c c a g a g g t g t a a a g t a c t t g a g t a a t t t t a c t t g a t t a c t g t a c t t a c c c g c t t a a c c c g g g t c t c c a c a t t t c a t g a a c t c a t t a a a a t g a a c t a a t g a c a t g a a t

120 a g t a t t a t t t t t g g g g a t t t t t a c t t t a c t t g a g t a c a a t t a a a a a t c a a t a c t t t t a c t t c a t a a t a a a a a c c c c t a a a a a t g a a a t g a a c t c a t g t t a a t t t t t a g t t a t g a a a a t g a

180 t t t a c t t a a t t a c a t t t t t t t a g a a a a a a a a g t a c t t t t t a c t c c t t a c a a t t t t a t t t a a a a t g a a t t a a t g t a a a a a a a t c t t t t t t t t c a t g a a a a a t g a g g a a t g t t a a a a t a a a t

240 c a g t c a a a a a g t a c t t a t t t t t t g g a g a t c a c t t c a t t c t a t t t t c c c t t g c t a t t a c c a g t c a g t t t t t c a t g a a t a a a a a a c c t c t a g t g a a g t a a g a t a a a a g g g a a c g a t a a t g g t

BglIISwaI 300 EF-1 α promoter *ISRAGTGT a a c c a a t t g a a t t g c g c t g a t g c c c a g t t t a a t t t a a a t a g a t c t g g c t c c g g t g c c c g t t t g g t t a a c t t a a c g c g a c t a c g g g t c a a a t t a a a t t t a t c t a g a c c g a g g c c a c g g g c a

360 EF-1 α promoter LPCLACRGCDGLLQPSPDAI c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a

420 EF-1 α promoter SGTGLSPPAPYVPFTIDHVP t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c

480 EF-1 α promoter EAKKGLTPSFRIYTCYDGHV c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g

540 EF-1 α promoter EF-1 α intron A NKKAVPKGGSCLYTGHTTGA g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g

600 EF-1 α promoter EF-1 α intron A PRAEKRTIARAHRSNSGGPE g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c t g g c t c c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g a c c g a g

660 EF-1 α promoter EF-1 α intron A LVHNKIGLQLWPRAKRKLLG c a g t a c g t g a t t c t t g a t c c c g a g c t g g a g c c a g g g g c g g g c c t t g c g c t t t a g g a g c c c g t c a t g c a c t a a g a a c t a g g g c t c g a c c t c g g t c c c c g c c c g g a a c g c g a a a t c c t c g g g

720 EF-1 α promoter EF-1 α intron A KAEHKLQPRAQASPGGRAFR c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g a c c c c g g c g g c g c a c g c t t a g a

780 EF-1 α promoter EF-1 α intron A TAGERRDRQKRYTELWKFNK g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a

840 EF-1 α promoter EF-1 α intron A IVQQSAKKRALYDQLHPGPD g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t t g t a a a t g c g g g c c a g g a t c c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a a c a t t t a c g c c c g g t c c t a g

900 EF-1 α promoter EF-1 α intron A ACQYKPKQPRPRRRPGHTGA t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g

960 EF-1 α promoter EF-1 α intron A CMNPSAPGALAAVSFRVPTT g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a

FseI 1020 EF-1 α promoter EF-1 α intron A ELQGAQEPAQGRAATYRGAR c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a

BstAPI 1080 EF-1 α promoter EF-1 α intron A PPLAPGTPVLQTLPFIAAER g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c

1140 EF-1 α promoter EF-1 α intron A GQELPSLISSAASPLAPPHT g c c c t g c t c c a g g g g g c t c a a a a t g g a g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c g g g a c g a g g t c c c c c g a g t t t t a c c t c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a

BbvCI 1200 EF-1 α promoter EF-1 α intron A VWVFSLPRETRLRRKM c a c c c a c a c a a a g g a a a g g g g c c t t t c c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g g t g g g t g t g t t t c c t t t c c c c g g a a a g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c

1260 EF-1 α promoter EF-1 α intron A a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t g g a g c t t t t g g a g t a c g t c g t c t t t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a c c t c g a a a a c c t c a t g c a g c a g a a

1320 EF-1 α promoter EF-1 α intron A t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c

1380 EF-1 α promoter EF-1 α intron A a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c

1440 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G T G G T T C EF1a-F g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c

BstBIBssHIIAscI 1500 EF-1 α promoter t g t c g t g a g g a t c a a a c c c g g g c t g c a g g a a t t c g g c g c g c c a c g c g t a c c g a t a g c t t c a c a g c a c t c c t a g t t t g g g c c c g a c g t c c t t a a g c c g c g c g g t g c g c a t g g c t a t c g a a g

EcoRV 1560 1 M 1a V Venus MV g a a t t c t g c a g t c g a c g a t a t c a c t a g t c t c g a g c a t t t t g g c a a a g a a t t c g g c a t g g t c t t a a g a c g t c a g c t g c t a t a g t g a t c a g a g c t c g t a a a a c c g t t t c t t a a g c c g t a c c a

1620 5 10 15 20 SKGEELFTGVVPILVELDGD Venus SKGEELFTGVVPILVELDGD g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t

1680 25 30 35 40 VNGHKFSVSGEGEGDATYGK Venus VNGHKFSVSGEGEGDATYGK c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g g c g a t g c c a c c t a c g g c a a g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t c c c g c t a c g g t g g a t g c c g t t

1740 45 50 55 60 LTLKLLCTTGKLPVPWPTLV Venus MHHRQAARALAHPR LTLKLLCTTGKLPVPWPTLV g c t g a c c c t g a a g c t t c t a t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t c g a c t g g g a c t t c g a a g a t a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a

1800 65 70 75 80 TTLGYGLQCFARYPDHMKQH Venus DHPGLRPAVLRPLPRPHEAA TTLGYGLQCFARYPDHMKQH g a c c a c c c t g g g c t a c g g c c t g c a g t g c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c t g g t g g g a c c c g a t g c c g g a c g t c a c g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t

1860 85 90 95 100 DFFKSAMPEGYVQERTIFFK Venus RLLQVRHARRLRPGAHHLLQ DFFKSAMPEGYVQERTIFFK c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t

1920 105 110 115 120 DDGNYKTRAEVKFEGDTLVN Venus C T G C T G C C G T T G A T G T T C T G EXFP-R GRRQLQDPRRGEVRGRHPGE DDGNYKTRAEVKFEGDTLVN g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t

1980 125 130 135 140 RIELKGIDFKEDGNILGHKL Venus PHRAEGHRLQGGRQHPGAQA RIELKGIDFKEDGNILGHKL c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a

2040 145 150 155 160 EYNYNSHNVYITADKQKNGI Venus GVQLQQPQRLYHRRQAEERH EYNYNSHNVYITADKQKNGI g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a c c g c c g a c a a g c a g a a g a a c g g c a t c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t g g c g g c t g t t c g t c t t c t t g c c g t a

2100 165 170 175 180 KANFKIRHNIEDGGVQLADH Venus QGQLQDPPQHRGRRRAARRP KANFKIRHNIEDGGVQLADH c a a g g c c a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g c g g c g t g c a g c t c g c c g a c c a g t t c c g g t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c g c c g c a c g t c g a g c g g c t g g t

2160 185 190 195 200 YQQNTPIGDGPVLLPDNHYL Venus LPAEHPHRRRPRAAARQPLP YQQNTPIGDGPVLLPDNHYL c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a

2220 205 210 215 220 SYQSALFKDPNEKRDHMVLL Venus ELPVRPVQRPQREARSHGPA SYQSALFKDPNEKRDHMVLL g a g c t a c c a g t c c g c c c t g t t c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t c t c g a t g g t c a g g c g g g a c a a g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a

2280 225 230 235 EFLTAAGITEGMNELY LEMA Venus GVPDRRRDH* EFLTAAGITEGMNELYLEMA g g a g t t c c t g a c c g c c g c c g g g a t c a c t g a g g g c a t g a a c g a g c t g t a c c t c g a g a t g g c c c t c a a g g a c t g g c g g c g g c c c t a g t g a c t c c c g t a c t t g c t c g a c a t g g a g c t c t a c c g

2340 (in frame with Venus) DEEKLPPGWEKRMSRSSGRV DEEKLPPGWEKRMSRSSGRV g g a c g a g g a g a a g c t g c c g c c c g g c t g g g a g a a g c g c a t g a g c c g c a g c t c a g g c c g a g t c c t g c t c c t c t t c g a c g g c g g g c c g a c c c t c t t c g c g t a c t c g g c g t c g a g t c c g g c t c a

2400 (in frame with Venus) YYFNHITNASQWERPSGNSS YYFNHITNASQWERPSGNSS g t a c t a c t t c a a c c a c a t c a c t a a c g c c a g c c a g t g g g a g c g g c c c a g c g g c a a c a g c a g c a t g a t g a a g t t g g t g t a g t g a t t g c g g t c g g t c a c c c t c g c c g g g t c g c c g t t g t c g t c

Acc65IKpnI 2460 (in frame with Venus) SGGKNGQGEPARGTSAGGSA SGGKNGQGEPARGTSAGGSA c a g t g g t g g c a a a a a c g g g c a g g g g g a g c c t g c c a g g g g t a c c a g t g c t g g t g g t a g t g c g t c a c c a c c g t t t t t g c c c g t c c c c c t c g g a c g g t c c c c a t g g t c a c g a c c a c c a t c a c g

2520 (in frame with Venus) GGSAGGSGSAGGSAGGSTSA GGSAGGSGSAGGSAGGSTSA t g g t g g t a g t g c t g g t g g t t c c g g c a g t g c t g g t g g t a g t g c t g g t g g t a g t a c c a g t g c a c c a c c a t c a c g a c c a c c a a g g c c g t c a c g a c c a c c a t c a c g a c c a c c a t c a t g g t c a c g

2580 (in frame with Venus) GGSAGGSAGGSAGGSAGGSG GGSAGGSAGGSAGGSAGGSG t g g t g g t a g t g c t g g t g g t a g t g c t g g t g g t a g t g c t g g t g g t a g t g c t g g t g g t t c c g g a c c a c c a t c a c g a c c a c c a t c a c g a c c a c c a t c a c g a c c a c c a t c a c g a c c a c c a a g g c c

2640 (in frame with Venus) SAGGSAGGSTSAGGSAGGSA SAGGSAGGSTSAGGSAGGSA c a g t g c t g g t g g t a g t g c t g g t g g t a g t a c c a g t g c t g g t g g t a g t g c t g g t g g t a g t g c g t c a c g a c c a c c a t c a c g a c c a c c a t c a t g g t c a c g a c c a c c a t c a c g a c c a c c a t c a c g

2700 (in frame with Venus) GGSAGGSAGGSGPDVPRAPV GGSAGGSAGGSGPDVPRAPV t g g t g g t a g t g c t g g t g g t a g t g c t g g t g g t t c c g g a c c a g a t g t c c c t a g a g c t c c a g t a c c a c c a t c a c g a c c a c c a t c a c g a c c a c c a a g g c c t g g t c t a c a g g g a t c t c g a g g t c a

NotIBamHI 2760 (in frame with Venus) DPAWLSFQFPGGRMVSKGEE DPAWLSFQFPGGRMVSKGEE *ELKRAAAHHTFPFF g g a t c c a g c a t g g c t g t c a t t c c a a t t t c c g g g c g g c c g c a t g g t g a g t a a a g g g g a a g a c c t a g g t c g t a c c g a c a g t a a g g t t a a a g g c c c g c c g g c g t a c c a c t c a t t t c c c c t t c t

2820 (in frame with Venus) LFTGVVPILVELDGDVNGHK LFTGVVPILVELDGDVNGHK QKSAHHWNQDFQISIYIPMF a c t g t t t a c t g g c g t g g t g c c a a t t c t g g t c g a a c t g g a t g g a g a t g t a a a t g g g c a t a a t g a c a a a t g a c c g c a c c a c g g t t a a g a c c a g c t t g a c c t a c c t c t a c a t t t a c c c g t a t t

2880 (in frame with Venus) FSVSGEGEGDATYGKLTLKF FSVSGEGEGDATYGKLTLKF KTHRSFPFSVSCIPFQCQFK a t t t a g t g t g t c t g g a g a a g g g g a a g g a g a c g c t a c a t a t g g g a a a c t g a c a c t g a a a t t t a a a t c a c a c a g a c c t c t t c c c c t t c c t c t g c g a t g t a t a c c c t t t g a c t g t g a c t t t a a

2940 (in frame with Venus) ICTTGKLPVPWPTLVTTLSW ICTTGKLPVPWPTLVTTLSW NTSSSFQRHRPRCEHCGQRP t a t t t g t a c t a c t g g a a a a c t g c c t g t g c c t t g g c c t a c a c t c g t g a c a a c c c t g t c t t g a t a a a c a t g a t g a c c t t t t g a c g g a c a c g g a a c c g g a t g t g a g c a c t g t t g g g a c a g a a c

3000 (in frame with Venus) GVQCFARYPDHMKQHDFFKS GVQCFARYPDHMKQHDFFKS SHLTKGPIRIMHFLMIKEFR g g g a g t g c a g t g t t t t g c c c g g t a t c c t g a t c a t a t g a a a c a g c a t g a t t t t t t c a a a t c c c c t c a c g t c a c a a a a c g g g c c a t a g g a c t a g t a t a c t t t g t c g t a c t a a a a a a g t t t a g

Bsu36I 3060 (in frame with Venus) AMPEGYVQERTIFFKDDGNY AMPEGYVQERTIFFKDDGNY GHRLSIDLFPCNEKFIIPII t g c c a t g c c t g a g g g a t a t g t c c a g g a a c g g a c a a t t t t c t t t a a a g a t g a t g g g a a t t a a c g g t a c g g a c t c c c t a t a c a g g t c c t t g c c t g t t a a a a g a a a t t t c t a c t a c c c t t a a t

3120 (in frame with Venus) KTRAEVKFEGDTLVNRIELK KTRAEVKFEGDTLVNRIELK FCPGFHFKFPICQHIPNFQF t a a a a c a c g g g c c g a a g t g a a a t t t g a a g g g g a t a c a c t g g t g a a t c g g a t t g a a c t g a a a t t t t g t g c c c g g c t t c a c t t t a a a c t t c c c c t a t g t g a c c a c t t a g c c t a a c t t g a c t t

3180 (in frame with Venus) GIDFKEDGNILGHKLEYNYF GIDFKEDGNILGHKLEYNYF SNIKFFIPINQAMFQFIIIE a g g a a t t g a t t t t a a a g a a g a t g g g a a t a t t c t g g g c c a t a a a c t g g a a t a t a a t t a t t t t c c t t a a c t a a a a t t t c t t c t a c c c t t a t a a g a c c c g g t a t t t g a c c t t a t a t t a a t a a a

3240 (in frame with Venus) SDNVYITADKQKNGIKANFK SDNVYITADKQKNGIKANFK AIIDVNCGIFLLIPNFGIKF c a g c g a t a a t g t c t a c a t t a c a g c c g a t a a a c a g a a g a a t g g g a t t a a a g c c a a t t t t a a g t c g c t a t t a c a g a t g t a a t g t c g g c t a t t t g t c t t c t t a c c c t a a t t t c g g t t a a a a t t

3300 (in frame with Venus) IRHNIEDGGVQLADHYQQNT IRHNIEDGGVQLADHYQQNT NPMINFISPHLEGIMILLIC a a t t c g g c a t a a t a t t g a a g a t g g a g g g g t g c a g c t c g c c g a t c a t t a t c a g c a g a a t a c t t a a g c c g t a t t a t a a c t t c t a c c t c c c c a c g t c g a g c g g c t a g t a a t a g t c g t c t t a t g

3360 (in frame with Venus) PIGDGPVLLPDNHYLSTQSA PIGDGPVLLPDNHYLSTQSA RNPIPRHQQRIIMIQACLRG a c c t a t t g g g g a t g g g c c t g t g c t g c t g c c t g a t a a t c a t t a t c t g a g c a c a c a g t c t g c t g g a t a a c c c c t a c c c g g a c a c g a c g a c g g a c t a t t a g t a a t a g a c t c g t g t g t c a g a c g

3420 (in frame with Venus) LSKDPNEKRDHMVLLEFLTA LSKDPNEKRDHMVLLEFLTA QALVRIFLPVM c c t g a g c a a g g a c c c t a a t g a a a a g c g g g a c c a t a t g g t c c t g c t g g a a t t t c t g a c t g c g g a c t c g t t c c t g g g a t t a c t t t t c g c c c t g g t a t a c c a g g a c g a c c t t a a a g a c t g a c g

3480 (in frame with Venus) AGITLGMDELSR 130 PKKKRKV * SV40 NLS AGITLGMDELSRPKKKRKV* c g c c g g c a t t a c a c t c g g g a t g g a t g a a c t g t c t a g a c c t a a g a a g a a g c g t a a g g t g t a g c g g c c g t a a t g t g a g c c c t a c c t a c t t g a c a g a t c t g g a t t c t t c t t c g c a t t c c a c a t

BmtINheI 3540 a g t c g a c g g t c g a g t c g a g a c g c t a g c c a c c g g t c g t t a a c a t t a a g c t a g a a c t a t a g t t c a g c t g c c a g c t c a g c t c t g c g a t c g g t g g c c a g c a a t t g t a a t t c g a t c t t g a t a t c a

SnaBI 3600 SV40 poly(A) signal C T A C T C A A A C C T G T T T G G T G EBV-rev g a g t c g t a t t a c g t a g a t c c a g a c a t g a t a a g a t a c a t t g a t g a g t t t g g a c a a a c c a c a c t c a g c a t a a t g c a t c t a g g t c t g t a c t a t t c t a t g t a a c t a c t c a a a c c t g t t t g g t g t

3660 SV40 poly(A) signal G A A A T T T G T G A T G C T A T T G C SV40pA-R a c t a g a a t g c a g t g a a a a a a a t g c t t t a t t t g t g a a a t t t g t g a t g c t a t t g c t t t a t t t t g a t c t t a c g t c a c t t t t t t t a c g a a a t a a a c a c t t t a a a c a c t a c g a t a a c g a a a t a a a

BspDIClaI 3720 SV40 poly(A) signal g t a a c c a t t a t a a g c t g c a a t a a a c a a g t t a a c a a c g c c g g c c a t c g a t t a a a c t c a g g t c a t t g g t a a t a t t c g a c g t t a t t t g t t c a a t t g t t g c g g c c g g t a g c t a a t t t g a g t c c a

3780 PGK promoter t c a g g g g g a g g t g t g g g a g g t t t t t t a a t t c t c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c c c c c t c c a c a c c c t c c a a a a a a t t a a g a g c c c a t c c c c t c c g c g a a a a g g g t t c c g

3840 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a

3900 PGK promoter g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g

3960 PGK promoter c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c

4020 PGK promoter c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t

BlpI 4080 PGK promoter g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a

4140 PGK promoter c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c

4200 PGK promoter C A T T C T G C A C G C T T C mPGK-F g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g

4260 PGK promoter A A A A G mPGK-F a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t g c a t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a c g t

4320 EM7 promoter g c c t g t t g a c a a t t a a t c a t c g g c a t a g t a t a t c g g c a t a g t a t a a t a c g a c a a g g t g a g c g g a c a a c t g t t a a t t a g t a g c c g t a t c a t a t a g c c g t a t c a t a t t a t g c t g t t c c a c t c

MscINcoI 4380 1 5 10 15 MAKPLSQEESTLIERAT BSD g a a c t a a a c c a t g g c c a a g c c t t t g t c t c a a g a a g a a t c c a c c c t c a t t g a a a g a g c a a c c t t g a t t t g g t a c c g g t t c g g a a a c a g a g t t c t t c t t a g g t g g g a g t a a c t t t c t c g t t g

4440 20 25 30 35 ATINSIPISEDYSVASAALS BSD g g c t a c a a t c a a c a g c a t c c c c a t c t c t g a a g a c t a c a g c g t c g c c a g c g c a g c t c t c t c c c g a t g t t a g t t g t c g t a g g g g t a g a g a c t t c t g a t g t c g c a g c g g t c g c g t c g a g a g a g

4500 40 45 50 55 SDGRIFTGVNVYHFTGGPCA BSD t a g c g a c g g c c g c a t c t t c a c t g g t g t c a a t g t a t a t c a t t t t a c t g g g g g a c c t t g t g c a t c g c t g c c g g c g t a g a a g t g a c c a c a g t t a c a t a t a g t a a a a t g a c c c c c t g g a a c a c g

4560 60 65 70 75 ELVVLGTAAAAAAGNLTCIV BSD a g a a c t c g t g g t g c t g g g c a c t g c t g c t g c t g c g g c a g c t g g c a a c c t g a c t t g t a t c g t t c t t g a g c a c c a c g a c c c g t g a c g a c g a c g a c g c c g t c g a c c g t t g g a c t g a a c a t a g c a

NruI* 4620 80 85 90 95 AIGNENRGILSPCGRCRQVL BSD c g c g a t c g g a a a t g a g a a c a g g g g c a t c t t g a g c c c c t g c g g a c g g t g c c g a c a g g t g c t g c g c t a g c c t t t a c t c t t g t c c c c g t a g a a c t c g g g g a c g c c t g c c a c g g c t g t c c a c g a

4680 100 105 110 115 LDLHPGIKAIVKDSDGQPTA BSD t c t c g a t c t g c a t c c t g g g a t c a a a g c c a t a g t g a a g g a c a g t g a t g g a c a g c c g a c g g c a g a g c t a g a c g t a g g a c c c t a g t t t c g g t a t c a c t t c c t g t c a c t a c c t g t c g g c t g c c g

4740 120 125 130 VGIRELLPSGYVWEG* BSD a g t t g g g a t t c g t g a a t t g c t g c c c t c t g g t t a t g t g t g g g a g g g c t a a c c c g g g a g a t g t c a a c c c t a a g c a c t t a a c g a c g g g a g a c c a a t a c a c a c c c t c c c g a t t g g g c c c t c t a c

4800 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g g g g a g g c t a a c t g a g c t c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c c c c c t c c g a t t g a c t c g a g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g

4860 bGH poly(A) signal a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t

4920 bGH poly(A) signal c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t

4980 bGH poly(A) signal t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a a t a g c a g g c a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g t t a t c g t c c g

PacI 5040 bGH poly(A) signal a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g t t a a t t a a g c t t a a a c a a g a a t c t t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c a a t t a a t t c g a a t t t g t t c t t a g a

5100 c t a g t t t t c t t t c t t g c t t t t a c t t t t a c t t c c t t a a t a c t c a a g t a c a a t t t t a a t g g a g a t c a a a a g a a a g a a c g a a a a t g a a a a t g a a g g a a t t a t g a g t t c a t g t t a a a a t t a c c t

5160 g t a c t t t t t t a c t t t t a c t c a a g t a a g a t t c t a g c c a g a t a c t t t t a c t t t t a a t t g a g t c a t g a a a a a a t g a a a a t g a g t t c a t t c t a a g a t c g g t c t a t g a a a a t g a a a a t t a a c t c a

5220 a a a a t t t t c c c t a a g t a c t t g t a c t t t c a c t t g a g t a a a a t t t t t g a g t a c t t t t t a c a c t t t t a a a a g g g a t t c a t g a a c a t g a a a g t g a a c t c a t t t t a a a a a c t c a t g a a a a a t g t g

NsiIBstXI 5280 SP6 promoter G A T A SP6 c t c t g c t c g a c c a t a t g g g a g a g c t c c c a a c g c g t t g g a t g c a t a g c t t g a g t a t t c t a t g a g a c g a g c t g g t a t a c c c t c t c g a g g g t t g c g c a a c c t a c g t a t c g a a c t c a t a a g a t a

5340 SP6 promoter M13 rev lac operator T C A C A G T G G A T T T A SP6 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A M13/pUC Reverse a g t g t c a c c t a a a t a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t t c a c a g t g g a t t t a t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a

5400 -10 -35 lac operator lac promoter T A G G C G A M13/pUC Reverse a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c

5460 CAP binding site c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c

5520 g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g

BspQISapI 5580 G A G C G A G T G A C T G A G C G A L4440 g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g

5640 g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t

5700 a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c

5760 ori c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a

5820 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t

5880 ori g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g

5940 ori t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a

6000 ori a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c

6060 ori c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c

6120 ori c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a

6180 ori t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g

6240 ori t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c

6300 ori c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g

6360 ori a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a

6420 a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t

6480 285 *WH AmpR *WH a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a

6540 280 275 270 265 KILSAGIEAIQRNREDMTAQ AmpR KILSAGIEAIQRNREDMTAQ g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a

6600 260 255 250 245 SGTTYIVVIRSPKGDPGLAA AmpR SGTTYIVVIRSPKGDPGLAA g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c

BsaI 6660 240 235 230 225 IIGRSGREGAGSKDAIFWGA AmpR IIGRSGREGAGSKDAIFWGA c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c

6720 220 215 210 205 PLASRLLPGAVKDAEMWDIL AmpR PLASRLLPGAVKDAEMWDIL c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t

6780 200 195 190 185 QQRSALTLLEGTLLKRLTTA AmpR QQRSALTLLEGTLLKRLTTA a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c

6840 180 175 170 165 MAVPMTTDREDNPIAENLEP AmpR MAVPMTTDREDNPIAENLEP c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c

6900 160 155 150 145 EWRDLRTVHDGMNHLFATLE AmpR EWRDLRTVHDGMNHLFATLE g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a

6960 140 135 130 125 KPGGITTLLLNAATNDSMTI AmpR KPGGITTLLLNAATNDSMTI c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t

7020 120 115 110 105 AASCLERVTMGDTLHKETVP AmpR AASCLERVTMGDTLHKETVP t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c

7080 100 95 90 85 SYEVLDNQSYHIRRGLQEQG AmpR SYEVLDNQSYHIRRGLQEQG g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g

7140 80 75 70 65 ADIRSLVAGCLLVKFTSMMP AmpR A T A A T A C C G C G C C A C A T A G C Amp-R ADIRSLVAGCLLVKFTSMMP c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c

7200 60 55 50 45 FREEPRFSELIKGSNLDLEI AmpR FREEPRFSELIKGSNLDLEI g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t

7260 40 35 30 25 YGVRAGLQDEADKVKVLTEP AmpR YGVRAGLQDEADKVKVLTEP t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c

7320 H 20 15 10 5 signal sequence AFVPLCFAAFFPILAVRFH AmpR HAFVPLCFAAFFPILAVRFH g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a

7380 1 signal sequence QISM AmpR AmpR promoter QISM g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g

7440 AmpR promoter t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t

7500 c a t t t c c c c g a a a a g t g c c a c c t g a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a g t a a a g g g g c t t t t c a c g g t g g a c t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t

7560 f1 ori a a a t a c c g c a t c a g g a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t c g c g t t a a a t t t t t t t t a t g g c g t a g t c c t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a g c g c a a t t t a a a a a

7620 f1 ori g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t

7680 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t

7740 f1 ori a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c c a c t a c t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g g t g a t g

7800 f1 ori g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c g g a c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g c c t

7860 f1 ori a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a g a a t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t c t t

7920 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c g c t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t g c g

7980 f1 ori t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t c c a t t c g c c a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a g g t a a g c g g

8040 a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c g c t a t t a c g c c a t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a g c g a t a a t g c g g t

8100 C C C A M13/pUC Forward g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c c a g g g t t t t c c c a c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g g t c c c a a a a g g g t

8151 M13 fwd T7 promoter G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a a t t g t a a t a c g a c t c a c t a t a c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t t a a c a t t a t g c t g a g t g a t a t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.