Addgene pBTK300 Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pBTK300 Sequencing Result


Map View

BspHI (2) AcuI (1579) AlwNI (1447) BsiEI (1371) ApaLI (1345) PspFI (1339) BseYI (1335) HaeII (1279) BciVI (1234) BssSI - BssSαI (1204) pBR322ori-F (1172 .. 1191) DrdI (1139) NspI (1035) AflIII - PciI (1031) TaqII (1001) BsaAI (19) MslI (42) Bpu10I (99) CAT-R (179 .. 198) TsoI (227) BspEI (322) ApoI (326) BsrDI (345) AclI (413) BpmI (448) PasI - PflMI * (558) EaeI (591) MscI (593) BtgI - NcoI - StyI (627) SspI (638) TatI (741) ScaI (743) EcoRV (778) Eco53kI (784) BanII - SacI (786) pBTK300 1749 bp

Sequence View

MslIBsaAIBspHI 5ʹ 3ʹ 60 g t c a t g a t t g a t c g g g c a c g t a a g a g g t t c c a a c t t t c a c c a t a a t g a a a t a a g a t c a c t c a g t a c t a a c t a g c c c g t g c a t t c t c c a a g g t t g a a a g t g g t a t t a c t t t a t t c t a g t g a

Bpu10I 120 1 MEK CmR MEK a c c g g g c g t a t t t t t t g a g t t a t c g a g a t t t t c a g g a g c t a a g g a a g c t a a a a t g g a g a a t g g c c c g c a t a a a a a a c t c a a t a g c t c t a a a a g t c c t c g a t t c c t t c g a t t t t a c c t c t t

180 5 10 15 20 KITGYTTVDISQWHRKEHFE CmR C T CAT-R KITGYTTVDISQWHRKEHFE a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a a g a a c a t t t t g a t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t t c t t g t a a a a c t

TsoI 240 25 30 35 40 AFQSVAQCTYNQTVQLDITA CmR C C G T A A A G T C A G T C A A C G CAT-R AFQSVAQCTYNQTVQLDITA g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t g g a t a t t a c g g c c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a c c t a t a a t g c c g

300 45 50 55 60 FLKTVKKNKHKFYPAFIHIL CmR FLKTVKKNKHKFYPAFIHIL c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t t a t t c a c a t t c t g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a a t a a g t g t a a g a

BsrDIBspEIApoI 360 65 70 75 80 ARLMNAHPEFRMAMKDGELV CmR ARLMNAHPEFRMAMKDGELV t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t t c g t a t g g c a a t g a a a g a c g g t g a g c t g g t a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a a g c a t a c c g t t a c t t t c t g c c a c t c g a c c a

AclI 420 85 90 95 100 IWDSVHPCYTVFHEQTETFS CmR IWDSVHPCYTVFHEQTETFS g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c t g a a a c g t t t t c c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g a c t t t g c a a a a g

BpmI 480 105 110 115 120 SLWSEYHDDFRQFLHIYSQD CmR SLWSEYHDDFRQFLHIYSQD a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a t a t t c g c a a g a t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a t a t a a g c g t t c t

540 125 130 135 140 VACYGENLAYFPKGFIENMF CmR VACYGENLAYFPKGFIENMF t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t g a g a a t a t g t t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a a c t c t t a t a c a a

EaeIMscIPflMI*PasI 600 145 150 155 160 FVSANPWVSFTSFDLNVANM CmR FVSANPWVSFTSFDLNVANM t t t c g t t t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a c g t g g c c a a t a t a a a g c a a a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t g c a c c g g t t a t a

SspINcoIStyIBtgI 660 165 170 175 180 DNFFAPVFTMGKYYTQGDKV CmR DNFFAPVFTMGKYYTQGDKV g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a a g g c g a c a a g g t c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t t c c g c t g t t c c a

720 185 190 195 200 LMPLAIQVHHAVCDGFHVGR CmR LMPLAIQVHHAVCDGFHVGR g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a g c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t c

EcoRVTatIScaI 780 205 210 215 220 MLNELQQYCDEWQGGA* CmR MLNELQQYCDEWQGGA* a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g t a a t t t g a t a t t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g c a t t a a a c t a t a

Eco53kIBanIISacI 840 c g a g c t c g c t t g g a c t c c t g t t g a t a g a t c c a g t a a t g a c c t c a g a a c t c c a t c t g g a t t g c t c g a g c g a a c c t g a g g a c a a c t a t c t a g g t c a t t a c t g g a g t c t t g a g g t a g a c c t a a

900 lambda t0 terminator t g t t c a g a a c g c t c g g t t g c c g c c g g g c g t t t t t t a t t g g t c t g g t c t c a c a g c t g a c a a a c a a g t c t t g c g a g c c a a c g g c g g c c c g c a a a a a a t a a c c a g a c c a g a g t g t c g a c t g t t

960 a t g c t c t t t c c c t a a a c t c c c c c c a t a a a a a a a c c c g c c g a a g c g g g t t t t t a c g t t a t t t a c g a g a a a g g g a t t t g a g g g g g g t a t t t t t t t g g g c g g c t t c g c c c a a a a a t g c a a t a a

TaqII 1020 t g c g g a t t a a c g a t t a c g g a t t g a g a c c g a c t a c g g t t a t c c a c a g a a t c a g g g g a t a a c a c g c c t a a t t g c t a a t g c c t a a c t c t g g c t g a t g c c a a t a g g t g t c t t a g t c c c c t a t t g

AflIIIPciINspI 1080 g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c

DrdI 1140 ori t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t

1200 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g

BciVIBssSαIBssSI 1260 ori t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g

HaeII 1320 ori c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c

BsiEIApaLIBseYIPspFI 1380 ori g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g

1440 ori t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t

AlwNI 1500 ori g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c

1560 ori a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c

AcuI 1620 ori a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a

1680 ori g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t

1740 g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t

3ʹ 5ʹ 1749 g g g a t t t t g c c c t a a a a c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

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Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.