Addgene pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W Sequencing Result


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T7 (8573 .. 8592) M13 fwd M13 Forward (8546 .. 8563) M13/pUC Forward (8532 .. 8554) F1ori-R (8299 .. 8318) F1ori-F (8087 .. 8108) PsiI (8046) Amp-R (7582 .. 7601) ScaI (7512) AhdI (7032) pBR322ori-F (6280 .. 6299) PciI (6139) L4440 (6029 .. 6046) CAP binding site M13/pUC Reverse (5811 .. 5833) lac operator M13 Reverse (5798 .. 5814) T3 (5759 .. 5779) T3 promoter PacI (5745) BGH-rev (5503 .. 5520) BclI * (5496) PmeI (5487) XcmI (5307) KpnI (5163) Acc65I (5159) Factor Xa site PflMI (4810) WPRE-R (4622 .. 4642) NotI (4564) T7 promoter SnaBI (231) NcoI (251) CMV-F (410 .. 430) NruI * (852) FseI (1169) EcoNI (1191) MfeI (1210) BbvCI (1445) AleI (1598) KflI (1955) PstI (2058) PspOMI (2246) ApaI (2250) MluI (2253) hU6-F (2276 .. 2296) LKO.1 5' (2447 .. 2466) BamHI (2638) MSCV-rev (2688 .. 2711) AgeI (2763) BlpI (2926) mPGK-F (3085 .. 3104) XbaI * (3164) PaeR7I - XhoI (3171) Puro-R (3192 .. 3211) PflFI - Tth111I (3234) BsiWI (3248) RsrII (3308) BstEII (3326) Puro-F (3688 .. 3708) CsiI - SexAI * (3759) BsrGI (3898) Bsu36I (4114) pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W 8650 bp

Sequence View

5ʹ 3ʹ 60 RGNVDIIVYTGM c c c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c g g g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g

120 c a t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g g t a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c

180 t a t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t a t a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a

SnaBI 240 t a t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g

NcoI 300 CMV promoter a t c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t t a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a

360 CMV promoter g a c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c t g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g

420 CMV promoter C G C A A A T G G G C CMV-F c a a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g t t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g

480 CMV promoter 5' LTR (truncated) G G T A G G C G T G CMV-F g g t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g c g c g t t t t g c c t g t a c t g g g t c t c t c c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c g c g c a a a a c g g a c a t g a c c c a g a g a

540 5' LTR (truncated) c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t

600 5' LTR (truncated) g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g

660 5' LTR (truncated) t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g

720 HIV-1 Ψ c c g a a c a g g g a c t t g a a a g c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g g g c t t g t c c c t g a a c t t t c g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c

780 HIV-1 Ψ c t t g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t g a a c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a

840 HIV-1 Ψ t t g a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a a c t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c

NruI* 900 a g a a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t c t t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t

960 t t a a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g a a t t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c

1020 t t a g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a a a t c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t

1080 g g a t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a c c t a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t

1140 a g g a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a t c c t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t

EcoNIFseI 1200 a g t a a g a c c a c c g c a c a g c a a g c g g c c g g c c g c g c t g a t c t t c a g a c c t g g a g g a g g a g a t c a t t c t g g t g g c g t g t c g t t c g c c g g c c g g c g c g a c t a g a a g t c t g g a c c t c c t c c t c t

MfeI 1260 MRDNWRSELYKYKVVKIEPL t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a

1320 GVAPTKAKRRVVQREKRAVG a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c

1380 RRE IGALFLGFLGAAGSTMGAAS a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g

1440 RRE MTLTVQARQLLSGIVQQQNN a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t

BbvCI 1500 RRE LLRAIEAQQHLLQLTVWGIK t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t

1560 RRE QLQARILAVERYLKDQQLLG g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c

AleI 1620 IWGCSGKLICTTAVPWNASW g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c

1680 SNKSLEQIWNHTTWMEWDRE g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t

1740 INNYTSLIHSLIEESQNQQE a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t

1800 1 5 10 15 KNEQELLELDKWASL WNWFN gp41 peptide KNEQELLELDKWASLWNWFN a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t

1860 (in frame with gp41 peptide) ITNWLWYIKLFIMIVGGLVG ITNWLWYIKLFIMIVGGLVG c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c

1920 (in frame with gp41 peptide) LRIVFAVLSIVNRVRQGYSP LRIVFAVLSIVNRVRQGYSP t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g

KflI 1980 (in frame with gp41 peptide) LSFQTHLPTPRGPDRPEGIE LSFQTHLPTPRGPDRPEGIE a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t

2040 (in frame with gp41 peptide) EEGGERDRDRSIRLVNGSAL EEGGERDRDRSIRLVNGSAL a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a

PstI 2100 (in frame with gp41 peptide) RAPILQTNGSIHPQF* cPPT/CTS RAPILQTNGSIHPQF* g c g t g c g c c a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g c g c a c g c g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c

2160 cPPT/CTS g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t

2220 cPPT/CTS c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t

PspOMIApaIMluI 2280 U6 promoter G A G G G hU6-F g c a g a g a t c c a g t t t g g t t a g t a c c g g g c c c t a c g c g t c c a a g g t c g g g c a g g a a g a g g g c g t c t c t a g g t c a a a c c a a t c a t g g c c c g g g a t g c g c a g g t t c c a g c c c g t c c t t c t c c c

2340 U6 promoter C C T A T T T C C C A T G A T T hU6-F c c t a t t t c c c a t g a t t c c t t c a t a t t t g c a t a t a c g a t a c a a g g c t g t t a g a g a g a t a a t g g a t a a a g g g t a c t a a g g a a g t a t a a a c g t a t a t g c t a t g t t c c g a c a a t c t c t c t a t t a

2400 U6 promoter t a g a a t t a a t t t g a c t g t a a a c a c a a a g a t a t t a g t a c a a a a t a c g t g a c g t a g a a a g t a a t c t t a a t t a a a c t g a c a t t t g t g t t t c t a t a a t c a t g t t t t a t g c a c t g c a t c t t t c a t

2460 U6 promoter G A C T A T C A T A T G C T LKO.1 5' a t a a t t t c t t g g g t a g t t t g c a g t t t t a a a a t t a t g t t t t a a a a t g g a c t a t c a t a t g c t t a t t a a a g a a c c c a t c a a a c g t c a a a a t t t t a a t a c a a a a t t t t a c c t g a t a g t a t a c g a

2520 U6 promoter T A C C G T LKO.1 5' t a c c g t a a c t t g a a a g t a t t t c g a t t t c t t g g c t t t a t a t a t c t t g t g g a a a g g a c g a a a a t g g c a t t g a a c t t t c a t a a a g c t a a a g a a c c g a a a t a t a t a g a a c a c c t t t c c t g c t t t

2580 U6 promoter c a c c g t c g g a c g c g g a c a c c a a g g g t t t a a g a g c t a t g c t g g a a a c a g c a t a g c a a g t t t g t g g c a g c c t g c g c c t g t g g t t c c c a a a t t c t c g a t a c g a c c t t t g t c g t a t c g t t c a a a

BamHI 2640 a a a t a a g g c t a g t c c g t t a t c a a c t t g a a a a a g t g g c a c c g a g t c g g t g c t t t t t t t g g a t t t a t t c c g a t c a g g c a a t a g t t g a a c t t t t t c a c c g t g g c t c a g c c a c g a a a a a a a c c t

2700 PGK promoter C G T A C G C G A A A T C MSCV-rev t c c a a t t c t a c c g g g t a g g g g a g g c g c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g a g g t t a a g a t g g c c c a t c c c c t c c g c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c

2760 PGK promoter G T C G G G G C G A C MSCV-rev c a g c c c c g c t g g g c a c t t g g c g c t a c a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t g t c g g g g c g a c c c g t g a a c c g c g a t g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a

AgeI 2820 PGK promoter c c a c c g g t a g g c g c c a a c c g g c t c c g t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c g g t g g c c a t c c g c g g t t g g c c g a g g c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g

2880 PGK promoter c t c c c c t a g t c a g g a a g t t c c c c c c c g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a g a g g g g a t c a g t c c t t c a a g g g g g g g c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t

BlpI 2940 PGK promoter MEA t g g a a g t a g c a c g t c t c a c t a g t c t c g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c a c c t t c a t c g t g c a g a g t g a t c a g a g c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g

3000 PGK promoter GRPLGQRPIAALLLRFLGSE g g g t a g g c c t t t g g g g c a g c g g c c a a t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a c c c a t c c g g a a a c c c c g t c g c c g g t t a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t

3060 PGK promoter AGKGWVRGRAQGRAQGRGGR g g c t g g g a a g g g g t g g g t c c g g g g g c g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c g a c c c t t c c c c a c c c a g g c c c c c g c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c

3120 PGK promoter C A T T C T G C A C G C T T C A A A A G mPGK-F PKVLRRPGILHASKAHVCRA c c c g a a g g t c c t c c g g a g g c c c g g c a t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c g g g c t t c c a g g a g g c c t c c g g g c c g t a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g

PaeR7IXhoIXbaI* 3180 PGK promoter VLLFLISGPFDLHPSRSRAA t g t t c t c c t c t t c c t c a t c t c c g g g c c t t t c g a c c t g c a t c c a t c t a g a t c t c g a g c a g c a c a a g a g g a g a a g g a g t a g a g g c c c g g a a a g c t g g a c g t a g g t a g a t c t a g a g c t c g t c g

Tth111IPflFI 3240 1 5 10 15 MTEYKPTVRLATRDDV PuroR T A C T G G C T C A T G T T C G G G T G Puro-R EAYHDRVQAHGAPRHPRRRP MTEYKPTVRLATRDDV t g a a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t c c a c t t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a g g

BsiWI 3300 20 25 30 35 PRAVRTLAAAFADYPATRHT PuroR QGRTHPRRRVRRLPRHAPHR PRAVRTLAAAFADYPATRHT c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c c g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g g c

BstEIIRsrII 3360 40 45 50 55 VDPDRHIERVTELQELFLTR PuroR RSGPPHRAGHRAARTLPHAR VDPDRHIERVTELQELFLTR t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g c g a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c g c

3420 60 65 70 75 VGLDIGKVWVADDGAAVAVW PuroR RARHRQGVGRGRRRRGGGLD VGLDIGKVWVADDGAAVAVW t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g a a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c c t

3480 80 85 90 95 TTPESVEAGAVFAEIGPRMA PuroR HAGERRSGGGVRRDRPAHGR TTPESVEAGAVFAEIGPRMA c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c g g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g g c

3540 100 105 110 115 ELSGSRLAAQQQMEGLLAPH PuroR VERFPAGRAATDGRPPGAAP ELSGSRLAAQQQMEGLLAPH a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a c c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t g g

3600 120 125 130 135 RPKEPAWFLATVGVSPDHQG PuroR AQGARVVPGHRRRLARPPGQ RPKEPAWFLATVGVSPDHQG g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g c a c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c g t

3660 140 145 150 155 KGLGSAVVLPGVEAAERAGV PuroR GSGQRRRAPRSGGGRARRGA KGLGSAVVLPGVEAAERAGV a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g c t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a c g

3720 160 165 170 175 PAFLETSAPRNLPFYERLGF PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F RLPGDLRAPQPPLLRAARLH PAFLETSAPRNLPFYERLGF c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c a g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a g t

SexAI*CsiI 3780 180 185 190 195 TVTADVEVPEGPRTWCMTRK PuroR RHRRRRGARRTAHLVHDPQA TVTADVEVPEGPRTWCMTRK c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g c g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t c g

3840 PGA (in frame with PuroR) GGGSGG 140 145 EGRGSLLTCGD PuroR T2A RCRRRVRRRGQRKSPNMR* PGAGGGSGGEGRGSLLTCGD *CATV c c g g t g c c g g c g g c g g g t c c g g a g g a g a g g g c a g a g g a a g t c t c c t a a c a t g c g g t g a c g g g c c a c g g c c g c c g c c c a g g c c t c c t c t c c c g t c t c c t t c a g a g g a t t g t a c g c c a c t g c

BsrGI 3900 150 155 VEENPGP 1 5 10 MSELIKENMHMKL T2A TagBFP VEENPGPMSELIKENMHMKL HLLIRAWHALQNLLVHVHLQ t g g a g g a g a a t c c t g g c c c a a t g a g c g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a c c t c c t c t t a g g a c c g g g t t a c t c g c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a

3960 15 20 25 30 YMEGTVDNHHFKCTSEGEGK TagBFP YMEGTVDNHHFKCTSEGEGK VHLAGHVVMVELACGLAFAL a c a t g g a g g g c a c c g t g g a c a a c c a t c a c t t c a a g t g c a c a t c c g a g g g c g a a g g c a a g c t g t a c c t c c c g t g g c a c c t g t t g g t a g t g a a g t t c a c g t g t a g g c t c c c g c t t c c g t t c g

4020 35 40 45 50 PYEGTQTMRIKVVEGGPLPF TagBFP PYEGTQTMRIKVVEGGPLPF GVLAGLGHSDLHDLAAREGE c c t a c g a g g g c a c c c a g a c c a t g a g a a t c a a g g t g g t c g a g g g c g g c c c t c t c c c c t t c g g g a t g c t c c c g t g g g t c t g g t a c t c t t a g t t c c a c c a g c t c c c g c c g g g a g a g g g g a a g c

4080 55 60 65 70 AFDILATSFLYGSKTFINHT TagBFP AFDILATSFLYGSKTFINHT GEVDQSSAEEVAALGEDVVG c c t t c g a c a t c c t g g c t a c t a g c t t c c t c t a c g g c a g c a a g a c c t t c a t c a a c c a c a c c c g g a a g c t g t a g g a c c g a t g a t c g a a g g a g a t g c c g t c g t t c t g g a a g t a g t t g g t g t g g g

Bsu36I 4140 75 80 85 90 QGIPDFFKQSFPEGFTWERV TagBFP QGIPDFFKQSFPEGFTWERV LADGVEELLGERLAECPLSD a g g g c a t c c c c g a c t t c t t c a a g c a g t c c t t c c c t g a g g g c t t c a c a t g g g a g a g a g t c a t c c c g t a g g g g c t g a a g a a g t t c g t c a g g a a g g g a c t c c c g a a g t g t a c c c t c t c t c a g t

4200 95 100 105 110 TTYEDGGVLTATQDTSLQDG TagBFP TTYEDGGVLTATQDTSLQDG GCVLVPAHQGSGLVGAELVA c c a c a t a c g a g g a c g g g g g c g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g g t g t a t g c t c c t g c c c c c g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a

4260 115 120 125 130 CLIYNVKIRGVNFTSNGPVM TagBFP CLIYNVKIRGVNFTSNGPVM AEDVVDLDSPHVECGVARHH g c c t c a t c t a c a a c g t c a a g a t c a g a g g g g t g a a c t t c a c a t c c a a c g g c c c t g t g a t g c c g g a g t a g a t g t t g c a g t t c t a g t c t c c c c a c t t g a a g t g t a g g t t g c c g g g a c a c t a c g

4320 135 140 145 150 QKKTLGWEAFTETLYPADGG TagBFP QKKTLGWEAFTETLYPADGG LLFCEAPLGEGLRQVGSVAA a g a a g a a a a c a c t c g g c t g g g a g g c c t t c a c c g a g a c g c t g t a c c c c g c t g a c g g c g g c c t c t t c t t t t g t g a g c c g a c c c t c c g g a a g t g g c t c t g c g a c a t g g g g c g a c t g c c g c c g g

4380 155 160 165 170 LEGRNDMALKLVGGSHLIAN TagBFP LEGRNDMALKLVGGSHLIAN QFASVVHGQLEHAPAM t g g a a g g c a g a a a c g a c a t g g c c c t g a a g c t c g t g g g c g g g a g c c a t c t g a t c g c a a a c a a c c t t c c g t c t t t g c t g t a c c g g g a c t t c g a g c a c c c g c c c t c g g t a g a c t a g c g t t t g t

4440 175 180 185 190 IKTTYRSKKPAKNLKMPGVY TagBFP IKTTYRSKKPAKNLKMPGVY t c a a g a c c a c a t a t a g a t c c a a g a a a c c c g c t a a g a a c c t c a a g a t g c c t g g c g t c t a c t a g t t c t g g t g t a t a t c t a g g t t c t t t g g g c g a t t c t t g g a g t t c t a c g g a c c g c a g a t g a

4500 195 200 205 210 YVDYRLERIKEANNETYVEQ TagBFP YVDYRLERIKEANNETYVEQ a t g t g g a c t a c a g a c t g g a a a g a a t c a a g g a g g c c a a c a a c g a g a c c t a c g t c g a g c a g c t a c a c c t g a t g t c t g a c c t t t c t t a g t t c c t c c g g t t g t t g c t c t g g a t g c a g c t c g t c g

4560 215 220 225 230 HEVAVARYCDLPSKLGHKLN TagBFP HEVAVARYCDLPSKLGHKLN a c g a g g t g g c a g t g g c c a g a t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a a g c t t a a t t t g c t c c a c c g t c a c c g g t c t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t t c g a a t t a a

NotI 4620 * TagBFP * g a g c g g c c g c t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t c t c g c c g g c g a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a

4680 A C A A C G A G G A A A A T G C G A T A C WPRE-R MLL MPLYHAI a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c

4740 LPVWLSFSPPCINPGCCLFM ASRMAFIFSSLYKSWLLSLY c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c

4800 RSCGPLSGNVAWCALCLLTQ EELWPVVRQRGVVCTVFADA a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t

PflMI 4860 PPLVGALPPPVSSFPGLSLS TPTGWGIATTCQLLSGTFAF c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g

4920 PSLLPRRNSSPPALPAAGQG PLPIATAELIAACLARCWTG c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c

4980 *RGKR LGCWALTIPWCCRGNHRPFL ARLLGTDNSVVLSGKSSSFP c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c a t c g t c c t t t c c t t g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g t a g c a g g a a a g g a a

5040 (in frame with Factor Xa site) PQEGTNGGPNQAPRGEAVDR Factor Xa site GCSPVLPPGFCAGRPSATSL WLLACVATWILRGTSFCYVP g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c c g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a

5100 1 RGEI Factor Xa site RPSIQRTFLPAACCRLCGLF SALNPADLPSRGLLPALRPL c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g

Acc65I 5160 RVYAFALRRVGSPFGPPPRR PRLRLRPQTSRISLWAASPQ c g c g t c t a c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c a g g g c g c a g a t g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g t c c

KpnI 5220 YL* VPLRPMTYKAAVDLSHFLKE t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t

5280 KGGLEGLIHSQRSQDLLFAC a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a a g a a g t c a a g a t c t g c t t t t t g c c t g t a t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t t c t t c a g t t c t a g a c g a a a a a c g g a c a t

XcmI 5340 TGSLWLDQSLSG* c t g g g t c t c t c t g g t t a g a c c a g a g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t g a c c c a g a g a g a c c a a t c t g g t c t c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a

5400 c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t

5460 a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t

BclI*PmeI 5520 bGH poly(A) signal G G A G C T G A C A C G G A A G A T BGH-rev g t c a g t g t g g a a a a t c t c t a g c a g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a c a g t c a c a c c t t t t a g a g a t c g t c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t

5580 bGH poly(A) signal g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t

5640 bGH poly(A) signal c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g

5700 bGH poly(A) signal a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g t c a a t a t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c a g t t a t

PacI 5760 bGH poly(A) signal T3 promoter G G T3 g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g g c g g c c g t t a a t t a a g c t t t t g t t c c c c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c c g c c g g c a a t t a a t t c g a a a a c a a g g g

5820 T3 promoter M13 rev A A A T C A C T C C C A A T T A A C G T3 C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T M13/pUC Reverse t t t a g t g a g g g t t a a t t g c g c g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a a a t c a c t c c c a a t t a a c g c g c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t

5880 -10 -35 lac operator lac promoter T A A C A A T A G G C G A M13/pUC Reverse a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g g a g c a t a a a g t g t a a a g c c t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c c t c g t a t t t c a c a t t t c g g a

5940 CAP binding site g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g

6000 a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c

6060 G A G C G A G T G A C T G A G C G A L4440 g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g

6120 g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c

PciI 6180 g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t

6240 ori a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g

6300 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g

6360 ori c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c

6420 ori c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a

6480 ori c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g

6540 ori g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g

6600 ori c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c

6660 ori a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c

6720 ori c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t

6780 ori c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c

6840 ori g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a

6900 c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t

6960 * AmpR * a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a

7020 285 280 275 270 WHKILSAGIEAIQRNREDMT AmpR WHKILSAGIEAIQRNREDMT a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c

AhdI 7080 265 260 255 250 AQSGTTYIVVIRSPKGDPGL AmpR AQSGTTYIVVIRSPKGDPGL t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t

7140 245 240 235 230 AAIIGRSGREGAGSKDAIFW AmpR AAIIGRSGREGAGSKDAIFW g t g c t g c a a t g a t a c c g c g a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c c a c g a c g t t a c t a t g g c g c t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g

7200 225 220 215 210 GAPLASRLLPGAVKDAEMWD AmpR GAPLASRLLPGAVKDAEMWD a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a

7260 205 200 195 190 ILQQRSALTLLEGTLLKRLT AmpR ILQQRSALTLLEGTLLKRLT c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c

7320 185 180 175 170 TAMAVPMTTDREDNPIAENL AmpR TAMAVPMTTDREDNPIAENL t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t

7380 165 160 155 150 EPEWRDLRTVHDGMNHLFAT AmpR EPEWRDLRTVHDGMNHLFAT g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c

7440 145 140 135 130 LEKPGGITTLLLNAATNDSM AmpR LEKPGGITTLLLNAATNDSM t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t

7500 125 120 115 110 TIAASCLERVTMGDTLHKET AmpR TIAASCLERVTMGDTLHKET t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c

ScaI 7560 105 100 95 90 VPSYEVLDNQSYHIRRGLQE AmpR VPSYEVLDNQSYHIRRGLQE t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a

7620 85 80 75 70 QGADIRSLVAGCLLVKFTSM AmpR A T A A T A C C G C G C C A C A T A G C Amp-R QGADIRSLVAGCLLVKFTSM c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t

7680 65 60 55 50 MPFREEPRFSELIKGSNLDL AmpR MPFREEPRFSELIKGSNLDL t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t

7740 45 40 35 30 EIYGVRAGLQDEADKVKVLT AmpR EIYGVRAGLQDEADKVKVLT g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c

7800 25 EPH 20 15 10 signal sequence AFVPLCFAAFFPILAVR AmpR EPHAFVPLCFAAFFPILAVR t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g

7860 5 1 signal sequence FHQISM AmpR AmpR promoter FHQISM g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a

7920 AmpR promoter a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g

7980 AmpR promoter f1 ori c g c g c a c a t t t c c c c g a a a a g t g c c a c c t a a a t t g t a a g c g t t a a t a t t t t g t t a a a a t t g c g c g t g t a a a g g g g c t t t t c a c g g t g g a t t t a a c a t t c g c a a t t a t a a a a c a a t t t t a a

8040 f1 ori c g c g t t a a a t t t t t g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t g c g c a a t t t a a a a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a

PsiI 8100 f1 ori G G T C A A A C C T T G T T F1ori-F c c c t t a t a a a t c a a a a g a a t a g a c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g g g a a t a t t t a g t t t t c t t a t c t g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t

8160 f1 ori C T C A G G T G F1ori-F g a g t c c a c t a t t a a a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c t c a g g t g a t a a t t t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c

8220 f1 ori *DLKKPDLHRL c g a t g g c c c a c t a c g t g a a c c a t c a c c c t a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a g c t a c c g g g t g a t g c a c t t g g t a g t g g g a t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t

8280 f1 ori ASFRFGLPLGRNLAQRPFGA a g c a c t a a a t c g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c t c g t g a t t t a g c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g

8340 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R FTALFSPFFAFPAPALASAL g a a c g t g g c g a g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g c t t g c a c c g c t c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c

8400 f1 ori TATVSRTVVVGAASLAGSCP t g t a g c g g t c a c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g a c a t c g c c a g t g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c

8460 f1 ori ADWEGNLSRLQQSPRDTRAE c g c g t c c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c g c g c a g g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g

8520 ESNRWSAFPPHAALRNLQTV t t c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c a a g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g

8580 M13 fwd T7 promoter C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward T A A T A C G A T7 GPNEWDRRQLVVALSRAYYS g c c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a g c g c g c g t a a t a c g a c g g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t c g c g c g c a t t a t g c t

8640 T7 promoter C T C A C T A T A G G G T7 ESYPSNVLVYSVTFPGGPQG c t c a c t a t a g g g c g a a t t g a c t a g t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g a g t g a t a t c c c g c t t a a c t g a t c a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g

3ʹ 5ʹ 8650 GLS g c c c a a c g a c c g g g t t g c t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.