Addgene ER-M-TAG (pGS555) Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: ER-M-TAG (pGS555) Sequencing Result

Map View

TspMI - XmaI (87) pBR322ori-F (10,960 .. 10,979) ori Amp-R (9658 .. 9677) SspI (9422) AmpR promoter pBRforEco (9249 .. 9267) SV40pro-F (8965 .. 8984) EBV-rev (8833 .. 8852) SV40 poly(A) signal SV40pA-R (8779 .. 8798) 3' LTR (ΔU3) HpaI (8359) BsmBI - Esp3I (8103) PaeR7I - PspXI - XhoI (8069) AfeI (8064) WPRE-R (7516 .. 7536) SV40 NLS SNAP-tag® P2A BsaBI * (6708) IRES-F (6220 .. 6239) PaqCI (6165) PmlI (6142) IRES IRES reverse (5993 .. 6010) 3xHA SmaI (89) CAP binding site lac promoter M13/pUC Reverse (238 .. 260) lac operator M13 Reverse (257 .. 273) M13 rev T3 (292 .. 312) T3 promoter SphI (430) RSV promoter 5' LTR (truncated) HIV-1 Ψ BspQI - SapI (1215) gp41 peptide KflI (2043) cPPT/CTS XbaI (2281) PshAI (2931) PacI (3220) EF-1α promoter FseI (4060) EF1a-F (4486 .. 4506) BstXI (4599) Kozak sequence BclI * (5064) BstEII (5185) mCherry-R (5175 .. 5193) mCherry-F (5443 .. 5462) XcmI (5716) ER-M-TAG (pGS555) 11,228 bp

Sequence View

5ʹ 3ʹ 60 c g a t a a c c c t a a t t c g a t a g c a t a t g c t t c c c g t t g g g t a a c a t a t g c t a t t g a a t t a g g g c t a t t g g g a t t a a g c t a t c g t a t a c g a a g g g c a a c c c a t t g t a t a c g a t a a c t t a a t c c

TspMIXmaISmaI 120 g t t a g t c t g g a t a g t a t a t a c t a c t a c c c g g g a a g c a t a t g c t a c c c g t t t a g g g t t c a c c a a t c a g a c c t a t c a t a t a t g a t g a t g g g c c c t t c g t a t a c g a t g g g c a a a t c c c a a g t g

180 CAP binding site c g g t g a t g c c g g c c a c g a t g c g t c c g g c g t a g a g g a t c t a a t g t g a g t t a g c t c a c t c a t g c c a c t a c g g c c g g t g c t a c g c a g g c c g c a t c t c c t a g a t t a c a c t c a a t c g a g t g a g t a

240 -35 -10 lac promoter lac operator A G C M13/pUC Reverse t a g g c a c c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c a t c c g t g g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g

300 lac operator M13 rev T3 promoter G G A T A A C A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A C T3 g g a t a a c a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t a t t g t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g

360 T3 promoter RSV promoter C C T C A C T A A A G G T3 c c t c a c t a a a g g g a a c a a a a g c t g g a g c t g c a a g c t t a a t g t a g t c t t a t g c a a t a c t c t g g a g t g a t t t c c c t t g t t t t c g a c c t c g a c g t t c g a a t t a c a t c a g a a t a c g t t a t g a g a

420 RSV promoter t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g c a a c a t g c c t t a c a a g g a g a g a a a a a g c a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c g t t g t a c g g a a t g t t c c t c t c t t t t t c g

SphI 480 RSV promoter a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g t a c g a t c g t g c c t t a t t a g g a a g g c a a c t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c a t g c t a g c a c g g a a t a a t c c t t c c g t t g

540 RSV promoter a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t g a a t t g c c g c a t t g c a g a g a t a t t g t a t t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a c t t a a c g g c g t a a c g t c t c t a t a a c a t a

600 RSV promoter 5' LTR (truncated) t t a a g t g c c t a g c t c g a t a c a t a a a c g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a a t t c a c g g a t c g a g c t a t g t a t t t g c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c

660 5' LTR (truncated) a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g

720 5' LTR (truncated) t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a

780 5' LTR (truncated) t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g c g a a a g g a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c g c t t t c c

840 HIV-1 Ψ g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g c a a g a g g c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c g t t c t c c

900 HIV-1 Ψ c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a a g g a g a g g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t t c c t c t c

960 HIV-1 Ψ a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g a a a a a a t t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c t t t t t t a

1020 t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g c a a g c a g a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c g t t c g t c

1080 g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t a g a c a c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a c a t c t g t

1140 a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t t a t a t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a g t a a t a t

1200 t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a c c a a g g a a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t g g t t c c t

BspQISapI 1260 a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c a a g c g g c t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g t t c g c c g

1320 MRDNWRSELY c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t a t a t a g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t a a t a t a t

1380 KYKVVKIEPLGVAPTKAKRR a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a g a a g a g t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c t c t t c t c

1440 RRE VVQREKRAVGIGALFLGFLG t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t t g g g a g a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g a a c c c t c

1500 RRE AAGSTMGAASMTLTVQARQL c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a c a a t t a t g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t g t t a a t a

1560 RRE LSGIVQQQNNLLRAIEAQQH t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a c a g c a t c a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t g t c g t a g

1620 RRE LLQLTVWGIKQLQARILAVE t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t g g a a a a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a c a c c t t t

1680 RRE RYLKDQQLLGIWGCSGKLIC g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c a t t t g c a c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g t a a a c g t

1740 TTAVPWNASWSNKSLEQIWN c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t g g a a t c g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a a c c t t a g

1800 HTTWMEWDREINNYTSLIHS a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a c a c t c c t t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t g t g a g g a

1860 1 5 10 KNEQELLELD gp41 peptide LIEESQNQQEKNEQELLELD t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a t t a g a t a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t a a t c t a t

1920 15 KWASL (in frame with gp41 peptide) WNWFNITNWLWYIKL gp41 peptide KWASLWNWFNITNWLWYIKL a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a a t t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t t t t a a t a

1980 (in frame with gp41 peptide) FIMIVGGLVGLRIVFAVLSI FIMIVGGLVGLRIVFAVLSI t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t c t a t a g a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a a g a t a t c

2040 (in frame with gp41 peptide) VNRVRQGYSPLSFQTHLPTP VNRVRQGYSPLSFQTHLPTP t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a c c c c g a a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t t g g g g c t

KflI 2100 (in frame with gp41 peptide) RGPDRPEGIEEEGGERDRDR RGPDRPEGIEEEGGERDRDR g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a g a c a g a t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t c t g t c t a

2160 (in frame with gp41 peptide) SIRLVNGSRRYRF* cPPT/CTS SIRLVNGSRRYRF* c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g g t t t t a a a a g a a a a g g g g g g a t t g g g g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c c a a a a t t t t c t t t t c c c c c c t a a c c c

2220 cPPT/CTS g g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a c c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t

2280 cPPT/CTS t t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t a t c g a t a c c g t c g a g t t t a c t c c c t a t a a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a t a g c t a t g g c a g c t c a a a t g a g g g a t a

XbaI 2340 t c t a g a c a a a t g c t t g t c c t t c c t t c c t g t a a c a c a g c c a t t a a a c c a g g a g c a t c g c c c a g a t c t g t t t a c g a a c a g g a a g g a a g g a c a t t g t g t c g g t a a t t t g g t c c t c g t a g c g g g

2400 t t c c c c g g c c c t c a g g t a a g a g g a c c a a a t a c c g t a g c c g t t t c c a a t t t c a g t c c t t t a a a g g g g c c g g g a g t c c a t t c t c c t g g t t t a t g g c a t c g g c a a a g g t t a a a g t c a g g a a a t

2460 g c g c c a c c t g g t g c t a a c t a c t c t a t c a c g c t t t t a t c c a a t a a c t a c c t t t g t a a a t t t c g c g g t g g a c c a c g a t t g a t g a g a t a g t g c g a a a a t a g g t t a t t g a t g g a a a c a t t t a a a

2520 c c t t t c a a a a g t t c t g g c c g g g c g c g g t g g c t c a c g c t t g t a a t c c c a g c a c t t t g t g a g g g a a a g t t t t c a a g a c c g g c c c g c g c c a c c g a g t g c g a a c a t t a g g g t c g t g a a a c a c t c

2580 g g g t c a g g a g t t c g a g a c a t t a a t a g g g a g g t g g t c c c t g c a g t t a c g c c a a t g a t a a c c c c c a g t c c t c a a g c t c t g t a a t t a t c c c t c c a c c a g g g a c g t c a a t g c g g t t a c t a t t g g

2640 c c c g c c a g a a a a a t c t t a g t a g c c t t c c c t t t t t g t t t t c c g t g c c c c a a c t c g g c g g a t g g g c g g t c t t t t t a g a a t c a t c g g a a g g g a a a a a c a a a a g g c a c g g g g t t g a g c c g c c t a

2700 t g a c t c g g c c c c t t c c g g a a a c a c c c g a a t c a a c t t c t a g t c a a a t t a t t g t t c a c g c c g a c t g a g c c g g g g a a g g c c t t t g t g g g c t t a g t t g a a g a t c a g t t t a a t a a c a a g t g c g g c

2760 c a a t g a c c c a c c c c t g g c c c g c g t c t g t g g a a c t g a c c c c t g g t g t a c a g g a g a g t t c g c g t t a c t g g g t g g g g a c c g g g c g c a g a c a c c t t g a c t g g g g a c c a c a t g t c c t c t c a a g c g

2820 *NKLEWSGRGWR t g c t g a a a g t g g t c c c a a a g g g g t a c t a g t t t t t a a g c t c c c a a c t c c c c c t c c c c c a g c a c g a c t t t c a c c a g g g t t t c c c c a t g a t c a a a a a t t c g a g g g t t g a g g g g g a g g g g g t c g

2880 RSSEVGECRLPSSTPPTRNQ g t c t g g a g g a t t c c a c a c c c t c g c a c c g c a g g g g c g a g g a a g t g g g c g g a g t c c g g t t t t c a g a c c t c c t a a g g t g t g g g a g c g t g g c g t c c c c g c t c c t t c a c c c g c c t c a g g c c a a a a

PshAI 2940 RWGSLSGLLFAVASSVGTVT g g c g c c a g c c g c t g a g g c t g c c a a g c a g a a a a g c c a c c g c t g a g g a g a c t c c g g t c a c t g c c g c g g t c g g c g a c t c c g a c g g t t c g t c t t t t c g g t g g c g a c t c c t c t g a g g c c a g t g a c

3000 RAGGGGERGKPVVPRWAAFP t c c t c g c c c c g c c t c c c c c t t c c c t c c c c t t g g g g a c c a c c g g g c g c c a c g c c g c g a a c g a g g a g c g g g g c g g a g g g g g a a g g g a g g g g a a c c c c t g g t g g c c c g c g g t g c g g c g c t t g c

3060 LHRPRRRRRGGGLAGIGLPR g t a a g t g c c g c g g t c g t c g g c g c c t c c g c c c t c c c c c t a g g g c c c c a a t t c c c a g c g g g c c a t t c a c g g c g c c a g c a g c c g c g g a g g c g g g a g g g g g a t c c c g g g g t t a a g g g t c g c c c g

3120 PARGRGGGPARGSGRGEHGG g c g g c g c g c g g c c c c t c c c c c c g c c g g g c g c g c g c c c g c t g c c c c g c c c t t c g t g g c c g c c g c c g c g c g c c g g g g a g g g g g g c g g c c c g c g c g c g g g c g a c g g g g c g g g a a g c a c c g g c g

3180 PTPPAVGGGPPGARLERSGG c c g g c g t g g g c g g t g c c a c c c c t c c c c c c g g c g g c c c c g c g c g c a g c t c c c g g c t c c c t c g g c c g c a c c c g c c a c g g t g g g g a g g g g g g c c g c c g g g g c g c g c g t c g a g g g c c g a g g g a g

PacI 3240 GESTAQATARLKILIAYRRG c c c c t t c g g a t g t g g c t t g a g c t g t a g c g c g g a g t t t a a t t a a a a t c g c g t a c c g t c g a c g g g g a a g c c t a c a c c g a a c t c g a c a t c g c g c c t c a a a t t a a t t t t a g c g c a t g g c a g c t g

3300 RRIPLDRGLKAFISLTKFLS c t c g a c g g a t c g g g a g a t c t c g a c c g a g c t t t g c a a a g a t g g a t a a a g t t t t a a a c a g a g g a g c t g c c t a g c c c t c t a g a g c t g g c t c g a a a c g t t t c t a c c t a t t t c a a a a t t t g t c t c

3360 EF-1 α promoter SDKCSISRRPRSLLPFQSRH a g g a a t c t t t g c a g c t a a t g g a c c t t c t a g g t c t t g a a a g g a g t g g g a a t t g g c t c c g g t t c c t t a g a a a c g t c g a t t a c c t g g a a g a t c c a g a a c t t t c c t c a c c c t t a a c c g a g g c c a

3420 EF-1 α promoter GDTPLACM g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g

3480 EF-1 α promoter g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c

3540 EF-1 α promoter t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g

3600 EF-1 α promoter EF-1 α intron A g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a

3660 EF-1 α promoter EF-1 α intron A t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c c a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g g

3720 EF-1 α promoter EF-1 α intron A t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c

3780 EF-1 α promoter EF-1 α intron A a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c c t g g g c g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g g a c c c g c g

3840 EF-1 α promoter EF-1 α intron A t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c

3900 EF-1 α promoter EF-1 α intron A t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a

3960 EF-1 α promoter EF-1 α intron A c t t g t a a a t g c g g g c c a a g a t c g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g g a a c a t t t a c g c c c g g t t c t a g c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c

4020 EF-1 α promoter EF-1 α intron A c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c

FseI 4080 EF-1 α promoter EF-1 α intron A g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a

4140 EF-1 α promoter EF-1 α intron A c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g

4200 EF-1 α promoter EF-1 α intron A g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c

4260 EF-1 α promoter EF-1 α intron A g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g

4320 EF-1 α promoter EF-1 α intron A a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a

4380 EF-1 α promoter EF-1 α intron A c t c g a t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g g a g c t a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c

4440 EF-1 α promoter EF-1 α intron A a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a

4500 EF-1 α promoter EF-1 α intron A T C A A G C C T C A G A C A G EF1a-F t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c

4560 EF-1 α promoter EF-1 α intron A T G G T T C EF1a-F t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g g a a t t a g c t t g a c c a a g c t t g a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c c t t a a t c g a a c t g g t t c g a a c

BstXI 4620 Kozak sequence g t a c c g a g c t c g g a t c c a c t a g t a a c g g c c g c c a g t g t g c t g g a a t t a t c a c a a g g c c g c c a t g g c t c g a g c c t a g g t g a t c a t t g c c g g c g g t c a c a c g a c c t t a a t a g t g t t c c g g c g

4680 Kozak sequence MAPSLWKGLVGIGLFALAH c a c c a t g g c g c c g t c g c t g t g g a a g g g g c t g g t g g g c a t c g g t c t c t t t g c c c t a g c c c a g t g g t a c c g c g g c a g c g a c a c c t t c c c c g a c c a c c c g t a g c c a g a g a a a c g g g a t c g g g t

4740 AAFSAAQHRSYMRLTEKEDE c g c c g c c t t t t c c g c t g c g c a g c a t c g t t c t t a t a t g c g a t t a a c a g a a a a a g a a g a t g a g c g g c g g a a a a g g c g a c g c g t c g t a g c a a g a a t a t a c g c t a a t t g t c t t t t t c t t c t a c t

4800 SLPIDIVLQTLLAFAVTCYG a t c a c t g c c a a t a g a t a t a g t t c t t c a g a c a c t t c t g g c c t t t g c a g t t a c c t g t t a c g g t a g t g a c g g t t a t c t a t a t c a a g a a g t c t g t g a a g a c c g g a a a c g t c a a t g g a c a a t g c c

4860 IVHIAGEFKDMDATSELKNK t a t a g t t c a t a t t g c a g g a g a g t t t a a a g a c a t g g a t g c c a c t t c a g a a c t g a a a a a t a a a t a t c a a g t a t a a c g t c c t c t c a a a t t t c t g t a c c t a c g g t g a a g t c t t g a c t t t t t a t t

4920 TFDTLRNHPSFYVFNHRGRV g a c a t t t g a t a c g t t a a g g a a t c a c c c a t c c t t t t a t g t a t t t a a t c a t c g t g g t c g a g t c t g t a a a c t a t g c a a t t c c t t a g t g g g t a g g a a a a t a c a t a a a t t a g t a g c a c c a g c t c a

4980 LFRPSDTANSSNQDALSSNT a c t t t t c c g g c c t t c g g a t a c a g c a a a t t c t t c a a a c c a a g a t g c a t t g t c c t c t a a c a c t g a a a a g g c c g g a a g c c t a t g t c g t t t a a g a a g t t t g g t t c t a c g t a a c a g g a g a t t g t g

5040 SLKLRKLESLRRGGGSGGGG a t c a t t g a a g t t a c g a a a a c t c g a a t c a c t g c g t c g t g g t g g t g g t t c t g g a g g c g g c g g t a g t a a c t t c a a t g c t t t t g a g c t t a g t g a c g c a g c a c c a c c a c c a a g a c c t c c g c c g c c

BclI* 5100 5 10 15 20 VSKGEAVIKEFMRFKVHME mScarlet SVSKGEAVIKEFMRFKVHME a t c a g t g a g c a a g g g c g a g g c a g t g a t c a a g g a g t t c a t g c g g t t c a a g g t g c a c a t g g a t a g t c a c t c g t t c c c g c t c c g t c a c t a g t t c c t c a a g t a c g c c a a g t t c c a c g t g t a c c t

5160 25 30 35 40 GSMNGHEFEIEGEGEGRPYE mScarlet GSMNGHEFEIEGEGEGRPYE g g g c t c c a t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c g a c c c g a g g t a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g c t

BstEII 5220 45 50 55 60 GTQTAKLKVTKGGPLPFSWD mScarlet G G T T C G A C T T C C A C T G G T T mCherry-R GTQTAKLKVTKGGPLPFSWD g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c t c c t g g g a c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g a g g a c c c t

5280 65 70 75 80 ILSPQFMYGSRAFTKHPADI mScarlet ILSPQFMYGSRAFTKHPADI c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a g g g c c t t c a c c a a g c a c c c c g c c g a c a t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t c c c g g a a g t g g t t c g t g g g g c g g c t g t a

5340 85 90 95 100 PDYYKQSFPEGFKWERVMNF mScarlet PDYYKQSFPEGFKWERVMNF c c c c g a c t a c t a t a a g c a g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t t g g g g c t g a t g a t a t t c g t c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g a a

5400 105 110 115 120 EDGGAVTVTQDTSLEDGTLI mScarlet EDGGAVTVTQDTSLEDGTLI c g a g g a c g g c g g c g c c g t g a c c g t g a c c c a g g a c a c c t c c c t g g a g g a c g g c a c c c t g a t g c t c c t g c c g c c g c g g c a c t g g c a c t g g g t c c t g t g g a g g g a c c t c c t g c c g t g g g a c t a

5460 125 130 135 140 YKVKLRGTNFPPDGPVMQKK mScarlet C C C C G T A A T G C A G A A G A A mCherry-F YKVKLRGTNFPPDGPVMQKK c t a c a a g g t g a a g c t c c g c g g c a c c a a c t t c c c t c c t g a c g g c c c c g t a a t g c a g a a g a a g a t g t t c c a c t t c g a g g c g c c g t g g t t g a a g g g a g g a c t g c c g g g g c a t t a c g t c t t c t t

5520 145 150 155 160 TMGWEASTERLYPEDGVLKG mScarlet G A mCherry-F TMGWEASTERLYPEDGVLKG g a c a a t g g g c t g g g a a g c g t c c a c c g a g c g g t t g t a c c c c g a g g a c g g c g t g c t g a a g g g c t g t t a c c c g a c c c t t c g c a g g t g g c t c g c c a a c a t g g g g c t c c t g c c g c a c g a c t t c c c

5580 165 170 175 180 DIKMALRLKDGGRYLADFKT mScarlet DIKMALRLKDGGRYLADFKT c g a c a t t a a g a t g g c c c t g c g c c t g a a g g a c g g c g g c c g c t a c c t g g c g g a c t t c a a g a c g c t g t a a t t c t a c c g g g a c g c g g a c t t c c t g c c g c c g g c g a t g g a c c g c c t g a a g t t c t g

5640 185 190 195 200 TYKAKKPVQMPGAYNVDRKL mScarlet TYKAKKPVQMPGAYNVDRKL c a c c t a c a a g g c c a a g a a g c c c g t g c a g a t g c c c g g c g c c t a c a a c g t c g a c c g c a a g t t g t g g a t g t t c c g g t t c t t c g g g c a c g t c t a c g g g c c g c g g a t g t t g c a g c t g g c g t t c a a

5700 205 210 215 220 DITSHNEEYTVVEQYERSEG mScarlet DITSHNEEYTVVEQYERSEG g g a c a t c a c c t c c c a c a a c g a g g a a t a c a c c g t g g t g g a a c a g t a c g a a c g c t c c g a g g g c c t g t a g t g g a g g g t g t t g c t c c t t a t g t g g c a c c a c c t t g t c a t g c t t g c g a g g c t c c c

XcmI 5760 225 230 RHSTGGMDELYK (in frame with mScarlet) GGSGGGS 1 Y mScarlet 3xHA RHSTGGMDELYKGGSGGGSY c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g g g c g g a t c a g g t g g t g g t t c t t a g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c c c g c c t a g t c c a c c a c c a a g a a t

5820 5 10 15 20 PYDVPDYAYPYDVPDYAYPY 3xHA PYDVPDYAYPYDVPDYAYPY c c c a t a c g a t g t t c c a g a t t a c g c t t a t c c c t a c g a c g t g c c t g a t t a t g c a t a c c c a t a g g g t a t g c t a c a a g g t c t a a t g c g a a t a g g g a t g c t g c a c g g a c t a a t a c g t a t g g g t a t

5880 25 DVPDYA * 3xHA IRES DVPDYA* t g a t g t c c c c g a c t a t g c c t a a a a a c t a a c g t t a c t g g c c g a a g c c g c t t g g a a t a a g g c a c t a c a g g g g c t g a t a c g g a t t t t t g a t t g c a a t g a c c g g c t t c g g c g a a c c t t a t t c c g

5940 IRES c g g t g t g c g t t t g t c t a t a t g t t a t t t t c c a c c a t a t t g c c g t c t t t t g g c a a t g t g a g g g c c a c a c g c a a a c a g a t a t a c a a t a a a a g g t g g t a t a a c g g c a g a a a a c c g t t a c a c t c c

6000 IRES G A G A G C G G IRES reverse g c c c g g a a a c c t g g c c c t g t c t t c t t g a c g a g c a t t c c t a g g g g t c t t t c c c c t c t c g c c c g g g c c t t t g g a c c g g g a c a g a a g a a c t g c t c g t a a g g a t c c c c a g a a a g g g g a g a g c g g

6060 IRES T T T C C T T A C G IRES reverse a a a g g a a t g c a a g g t c t g t t g a a t g t c g t g a a g g a a g c a g t t c c t c t g g a a g c t t c t t g a t t t c c t t a c g t t c c a g a c a a c t t a c a g c a c t t c c t t c g t c a a g g a g a c c t t c g a a g a a c t

6120 IRES a g a c a a a c a a c g t c t g t a g c g a c c c t t t g c a g g c a g c g g a a c c c c c c a c c t g g c g a c a g g t c t g t t t g t t g c a g a c a t c g c t g g g a a a c g t c c g t c g c c t t g g g g g g t g g a c c g c t g t c c

PaqCIPmlI 6180 IRES t g c c t c t g c g g c c a a a a g c c a c g t g t a t a a g a t a c a c c t g c a a a g g c g g c a c a a c c c c a g a c g g a g a c g c c g g t t t t c g g t g c a c a t a t t c t a t g t g g a c g t t t c c g c c g t g t t g g g g t c

6240 IRES T G G C T C T C C T C A A G C G T A T T IRES-F t g c c a c g t t g t g a g t t g g a t a g t t g t g g a a a g a g t c a a a t g g c t c t c c t c a a g c g t a t t c a c g g t g c a a c a c t c a a c c t a t c a a c a c c t t t c t c a g t t t a c c g a g a g g a g t t c g c a t a a g

6300 IRES a a c a a g g g g c t g a a g g a t g c c c a g a a g g t a c c c c a t t g t a t g g g a t c t g a t c t g g g g c c t t t g t t c c c c g a c t t c c t a c g g g t c t t c c a t g g g g t a a c a t a c c c t a g a c t a g a c c c c g g a

6360 IRES c g g t g c a c a t g c t t t a c a t g t g t t t a g t c g a g g t t a a a a a a a c g t c t a g g c c c c c c g a a c g c c a c g t g t a c g a a a t g t a c a c a a a t c a g c t c c a a t t t t t t t g c a g a t c c g g g g g g c t t g

6420 1 MAKP IRES BSD MATMAKP c a c g g g g a c g t g g t t t t c c t t t g a a a a a c a c g a t g a t a a t a t g g c c a c a a t g g c c a a g c c g t g c c c c t g c a c c a a a a g g a a a c t t t t t g t g c t a c t a t t a t a c c g g t g t t a c c g g t t c g g

6480 5 10 15 20 LSQEESTLIERATATINSIP BSD LSQEESTLIERATATINSIP t t t g t c t c a a g a a g a a t c c a c c c t c a t t g a a a g a g c a a c g g c t a c a a t c a a c a g c a t c c c a a a c a g a g t t c t t c t t a g g t g g g a g t a a c t t t c t c g t t g c c g a t g t t a g t t g t c g t a g g g

6540 25 30 35 40 ISEDYSVASAALSSDGRIFT BSD ISEDYSVASAALSSDGRIFT c a t c t c t g a a g a c t a c a g c g t c g c c a g c g c a g c t c t c t c t a g c g a c g g c c g c a t c t t c a c g t a g a g a c t t c t g a t g t c g c a g c g g t c g c g t c g a g a g a g a t c g c t g c c g g c g t a g a a g t g

6600 45 50 55 60 GVNVYHFTGGPCAELVVLGT BSD GVNVYHFTGGPCAELVVLGT t g g t g t c a a t g t a t a t c a t t t t a c t g g g g g a c c t t g t g c a g a a c t c g t g g t g c t g g g c a c a c c a c a g t t a c a t a t a g t a a a a t g a c c c c c t g g a a c a c g t c t t g a g c a c c a c g a c c c g t g

6660 65 70 75 80 AAAAAAGNLTCIVAIGNENR BSD AAAAAAGNLTCIVAIGNENR t g c t g c t g c t g c g g c a g c t g g c a a c c t g a c t t g t a t c g t c g c g a t c g g a a a t g a g a a c a g a c g a c g a c g a c g c c g t c g a c c g t t g g a c t g a a c a t a g c a g c g c t a g c c t t t a c t c t t g t c

BsaBI* 6720 85 90 95 100 GILSPCGRCRQVLLDLHPGI BSD GILSPCGRCRQVLLDLHPGI g g g c a t c t t g a g c c c c t g c g g a c g g t g c c g a c a g g t g c t t c t c g a t c t g c a t c c t g g g a t c c c g t a g a a c t c g g g g a c g c c t g c c a c g g c t g t c c a c g a a g a g c t a g a c g t a g g a c c c t a

6780 105 110 115 120 KAIVKDSDGQPTAVGIRELL BSD KAIVKDSDGQPTAVGIRELL c a a a g c c a t a g t g a a g g a c a g t g a t g g a c a g c c g a c g g c a g t t g g g a t t c g t g a a t t g c t g t t t c g g t a t c a c t t c c t g t c a c t a c c t g t c g g c t g c c g t c a a c c c t a a g c a c t t a a c g a

6840 125 130 PSGYVWEG GSG 1 5 ATNFSLLKQ BSD P2A PSGYVWEGGSGATNFSLLKQ g c c c t c t g g t t a t g t g t g g g a g g g c g g a t c c g g c g c a a c a a a c t t c t c t c t g c t g a a a c a c g g g a g a c c a a t a c a c a c c c t c c c g c c t a g g c c g c g t t g t t t g a a g a g a g a c g a c t t t g t

6900 10 15 AGDVEENPGP GSG 1 5 MDKDCEM P2A SNAP-tag® AGDVEENPGPGSGMDKDCEM a g c c g g a g a t g t c g a a g a g a a t c c t g g a c c g g g a t c c g g c a t g g a c a a a g a c t g c g a a a t t c g g c c t c t a c a g c t t c t c t t a g g a c c t g g c c c t a g g c c g t a c c t g t t t c t g a c g c t t t a

6960 10 15 20 25 KRTTLDSPLGKLELSGCEQG SNAP-tag® KRTTLDSPLGKLELSGCEQG g a a g c g c a c c a c c c t g g a t a g c c c t c t g g g c a a g c t g g a a c t g t c t g g g t g c g a a c a g g g c t t c g c g t g g t g g g a c c t a t c g g g a g a c c c g t t c g a c c t t g a c a g a c c c a c g c t t g t c c c

7020 30 35 40 45 LHRIIFLGKGTSAADAVEVP SNAP-tag® LHRIIFLGKGTSAADAVEVP c c t g c a c c g t a t c a t c t t c c t g g g c a a a g g a a c a t c t g c c g c c g a c g c c g t g g a a g t g c c g g a c g t g g c a t a g t a g a a g g a c c c g t t t c c t t g t a g a c g g c g g c t g c g g c a c c t t c a c g g

7080 50 55 60 65 APAAVLGGPEPLMQATAWLN SNAP-tag® APAAVLGGPEPLMQATAWLN t g c c c c a g c c g c c g t g c t g g g c g g a c c a g a g c c a c t g a t g c a g g c c a c c g c c t g g c t c a a a c g g g g t c g g c g g c a c g a c c c g c c t g g t c t c g g t g a c t a c g t c c g g t g g c g g a c c g a g t t

7140 70 75 80 85 AYFHQPEAIEEFPVPALHHP SNAP-tag® AYFHQPEAIEEFPVPALHHP c g c c t a c t t t c a c c a g c c t g a g g c c a t c g a g g a g t t c c c t g t g c c a g c c c t g c a c c a c c c g c g g a t g a a a g t g g t c g g a c t c c g g t a g c t c c t c a a g g g a c a c g g t c g g g a c g t g g t g g g

7200 90 95 100 105 VFQQESFTRQVLWKLLKVVK SNAP-tag® VFQQESFTRQVLWKLLKVVK a g t g t t c c a g c a g g a g a g c t t t a c c c g c c a g g t g c t g t g g a a a c t g c t g a a a g t g g t g a a t c a c a a g g t c g t c c t c t c g a a a t g g g c g g t c c a c g a c a c c t t t g a c g a c t t t c a c c a c t t

7260 110 115 120 125 FGEVISYSHLAALAGNPAAT SNAP-tag® FGEVISYSHLAALAGNPAAT g t t c g g a g a g g t c a t c a g c t a c a g c c a c c t g g c c g c c c t g g c c g g c a a t c c c g c c g c c a c c a a g c c t c t c c a g t a g t c g a t g t c g g t g g a c c g g c g g g a c c g g c c g t t a g g g c g g c g g t g

7320 130 135 140 145 AAVKTALSGNPVPILIPCHR SNAP-tag® AAVKTALSGNPVPILIPCHR c g c c g c c g t g a a a a c c g c c c t g a g c g g a a a t c c c g t g c c c a t t c t g a t c c c c t g c c a c c g g c g g c g g c a c t t t t g g c g g g a c t c g c c t t t a g g g c a c g g g t a a g a c t a g g g g a c g g t g g c

7380 150 155 160 165 VVQGDLDVGGYEGGLAVKEW SNAP-tag® VVQGDLDVGGYEGGLAVKEW g g t g g t g c a g g g c g a c c t g g a c g t g g g g g g c t a c g a g g g c g g g c t c g c c g t g a a a g a g t g c c a c c a c g t c c c g c t g g a c c t g c a c c c c c c g a t g c t c c c g c c c g a g c g g c a c t t t c t c a c

7440 170 175 180 LLAHEGHRLGKPGLG GGS PK SNAP-tag® SV40 NLS LLAHEGHRLGKPGLGGGSPK g c t g c t g g c c c a c g a g g g c c a c a g a c t g g g c a a g c c t g g g c t g g g t g g c g g g a g c c c a a a c g a c g a c c g g g t g c t c c c g g t g t c t g a c c c g t t c g g a c c c g a c c c a c c g c c c t c g g g t t t

7500 130 KKRKV * SV40 NLS WPRE KKRKV* g a a g a a g c g g a a g g t g t a g c g g a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c c t t c t t c g c c t t c c a c a t c g c c t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g

7560 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R MPL t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a

7620 WPRE YHAIASRMAFIFSSLYKSWL MLLLPVWLSFSPPCINPG g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a

7680 WPRE LSLYEELWPVVRQRGVVCTV CCLFMRSCGPLSGNVAWCAL g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a

7740 WPRE FADATPTGWGIATTCQLLSG CLLTQPPLVGALPPPVSSFP g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c

7800 WPRE TFAFPLPIATAELIAACLAR ELSLSPSLLPRRNSSPPALP a a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g t t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c

7860 WPRE * CWTGARLLGTDNSVVLSGKS AAGQGLGCWALTIPWCCRGN c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a a t c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t t a g

7920 WPRE (in frame with Factor Xa site) RGKRPQEGINGGPNQAPRGE SSFPWLLAYVATWILRGTSF HRPFLGCSPMLPPGFCAGRP a t c g t c c t t t c c t t g g c t g c t c g c c t a t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t t a g c a g g a a a g g a a c c g a c g a g c g g a t a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a

7980 WPRE AVDR 1 RGEI Factor Xa site CYVPSALNPADLPSRGLLPA SATSLRPSIQRTFLPAACCR c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g

8040 WPRE LRPLPRLRLRPQTSRISLWA LCGLFRVFAFALRRVGSPFG t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g

PaeR7IPspXIXhoIAfeI 8100 WPRE ASPHRYRALLEVRQQSEIPS PPPRIDTERCSRYANRARFR c g c c t c c c c g c a t c g a t a c c g a g c g c t g c t c g a g g t a c g c c a a c a g a g c g a g a t t c c g t c g c g g a g g g g c g t a g c t a t g g c t c g c g a c g a g c t c c a t g c g g t t g t c t c g c t c t a a g g c a g

BsmBIEsp3I 8160 QRKKK* LKEKKSNEMNKMSPRASKCR t c a a a g a a a a a a a a a g t a a t g a a a t g a a t a a a a t g a g t c c t a g a g c c a g t a a a t g t c g t a a g t t t c t t t t t t t t t c a t t a c t t t a c t t a t t t t a c t c a g g a t c t c g g t c a t t t a c a g c a t

8220 KCLS* a a t g t c t c a g c t a g t c a g g t a g t a a a a g g t c t c a a c t a g g c a g t g g c a g a g c a g g a t t c a t t a c a g a g t c g a t c a g t c c a t c a t t t t c c a g a g t t g a t c c g t c a c c g t c t c g t c c t a a g t

8280 a a t t c a g g g c t g t t g t g a t g c c t c c g c a g a c t c t g a g c g c c a c c t g g t g g t a a t t t g t c t t t a a g t c c c g a c a a c a c t a c g g a g g c g t c t g a g a c t c g c g g t g g a c c a c c a t t a a a c a g a

8340 g t g c c t c t t c t g a c g t t g a a g a a c a g c a a c t a a c a c a c t a a c a c g g c a t t t a c t a t g g g c c a c g g a g a a g a c t g c a a c t t c t t g t c g t t g a t t g t g t g a t t g t g c c g t a a a t g a t a c c c g

HpaI 8400 c a g c c a t t g t t c t a a t g t t a a c t g t g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g g t c g g t a a c a a g a t t a c a a t t g a c a c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c

8460 3' LTR ( Δ U3) t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a a t t c a c t c c c a a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t t a a g t g a g g g t t g

8520 3' LTR ( Δ U3) g a a g a c a a g a t c t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t c t t c t g t t c t a g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a

8580 3' LTR ( Δ U3) g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c

8640 3' LTR ( Δ U3) t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g

8700 3' LTR ( Δ U3) c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a

8760 SV40 poly(A) signal a t t t a t a a c t t g c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t t a a a t a t t g a a c g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a

8820 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g

8880 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g

8940 t a t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c a t a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g

9000 T A T T T A T G C A G A G G C C G A G G SV40pro-F g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g g a t c c c t t g a g t g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c c t a g g g a a c t c a c

9060 g c t t t c a t c c t g g a g c a g a c t t t g c a g t c t g t g g a c t g c a a c a c a a c a t t g c c t t t a t g t c g a a a g t a g g a c c t c g t c t g a a a c g t c a g a c a c c t g a c g t t g t g t t g t a a c g g a a a t a c a

9120 g t a a c t c t t g g c t g a a g c t c t t a c a c c a a t g c t g g g g g a c a t g t a c c t c c c a g g g g c c c a c a t t g a g a a c c g a c t t c g a g a a t g t g g t t a c g a c c c c c t g t a c a t g g a g g g t c c c c g g g t

9180 g g a a g a c t a c g g g a g g c t a c a c c a a c g t c a a t c a g a g g g g c c t g t g t a g c t a c c g a t a a g c c t t c t g a t g c c c t c c g a t g t g g t t g c a g t t a g t c t c c c c g g a c a c a t c g a t g g c t a t t c

9240 c g g a c c c t c a a g a g g g c a t t a g c a a t a g t g t t t a t a a g g c c c c c t t g t t a a t t c t t g a a g g c c t g g g a g t t c t c c c g t a a t c g t t a t c a c a a a t a t t c c g g g g g a a c a a t t a a g a a c t t c

9300 C G G A G C A C T A T G C G G A T A A pBRforEco a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g t t a a t g t c a t g a t a a t a a t g g t t t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c a a t t a c a g t a c t a t t a t t a c c a a a g

9360 AmpR promoter t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c g c g g a a c c c c t a t t t g t t t a t t t t t a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g c g c c t t g g g g a t a a a c a a a t a a a a a

9420 AmpR promoter c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c a a t a a c c c t g a t a a a t g c t t c a a t a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g t t a t t g g g a c t a t t t a c g a a g t t a t

SspI 9480 1 5 10 signal sequence MSIQHFRVALIPFF AmpR promoter AmpR MSIQHFRVALIPFF a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t c c g t g t c g c c c t t a t t c c c t t t t t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a a g g c a c a g c g g g a a t a a g g g a a a a a

9540 15 20 signal sequence AAFCLPVFA 25 30 HPETLVKVKDA AmpR AAFCLPVFAHPETLVKVKDA t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a a a c g c t g g t g a a a g t a a a a g a t g c a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c t t t g c g a c c a c t t t c a t t t t c t a c g

9600 35 40 45 50 EDQLGARVGYIELDLNSGKI AmpR EDQLGARVGYIELDLNSGKI t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g a a c t g g a t c t c a a c a g c g g t a a g a t a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c t t g a c c t a g a g t t g t c g c c a t t c t a

9660 55 60 65 70 LESFRPEERFPMMSTFKVLL AmpR C G A Amp-R LESFRPEERFPMMSTFKVLL c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t g a t g a g c a c t t t t a a a g t t c t g c t g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t a c t a c t c g t g a a a a t t t c a a g a c g a

9720 75 80 85 90 CGAVLSRVDAGQEQLGRRIH AmpR T A C A C C G C G C C A T A A T A Amp-R CGAVLSRVDAGQEQLGRRIH a t g t g g c g c g g t a t t a t c c c g t g t t g a c g c c g g g c a a g a g c a a c t c g g t c g c c g c a t a c a t a c a c c g c g c c a t a a t a g g g c a c a a c t g c g g c c c g t t c t c g t t g a g c c a g c g g c g t a t g t

9780 95 100 105 110 YSQNDLVEYSPVTEKHLTDG AmpR YSQNDLVEYSPVTEKHLTDG c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t c a c a g a a a a g c a t c t t a c g g a t g g g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c a g t g t c t t t t c g t a g a a t g c c t a c c

9840 115 120 125 130 MTVRELCSAAITMSDNTAAN AmpR MTVRELCSAAITMSDNTAAN c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a c c a t g a g t g a t a a c a c t g c g g c c a a g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t g g t a c t c a c t a t t g t g a c g c c g g t t

9900 135 140 145 150 LLLTTIGGPKELTAFLHNMG AmpR LLLTTIGGPKELTAFLHNMG c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c t a a c c g c t t t t t t g c a c a a c a t g g g g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g a t t g g c g a a a a a a c g t g t t g t a c c c

9960 155 160 165 170 DHVTRLDRWEPELNEAIPND AmpR DHVTRLDRWEPELNEAIPND g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a g c t g a a t g a a g c c a t a c c a a a c g a c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c t c g a c t t a c t t c g g t a t g g t t t g c t

10,020 175 180 185 190 ERDTTMPAAMATTLRKLLTG AmpR ERDTTMPAAMATTLRKLLTG c g a g c g t g a c a c c a c g a t g c c t g c a g c a a t g g c a a c a a c g t t g c g c a a a c t a t t a a c t g g g c t c g c a c t g t g g t g c t a c g g a c g t c g t t a c c g t t g t t g c a a c g c g t t t g a t a a t t g a c c

10,080 195 200 205 210 ELLTLASRQQLIDWMEADKV AmpR ELLTLASRQQLIDWMEADKV c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a t a g a c t g g a t g g a g g c g g a t a a a g t g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t a t c t g a c c t a c c t c c g c c t a t t t c a

10,140 215 220 225 230 AGPLLRSALPAGWFIADKSG AmpR AGPLLRSALPAGWFIADKSG t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g g c t g g t t t a t t g c t g a t a a a t c t g g a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c c g a c c a a a t a a c g a c t a t t t a g a c c

10,200 235 240 245 250 AGERGSRGIIAALGPDGKPS AmpR AGERGSRGIIAALGPDGKPS a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c a c t g g g g c c a g a t g g t a a g c c c t c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c g t g a c c c c g g t c t a c c a t t c g g g a g

10,260 255 260 265 270 RIVVIYTTGSQATMDERNRQ AmpR RIVVIYTTGSQATMDERNRQ c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g c a a c t a t g g a t g a a c g a a a t a g a c a g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c g t t g a t a c c t a c t t g c t t t a t c t g t

10,320 275 280 285 IAEIGASLIKHW* AmpR IAEIGASLIKHW* g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g g t a a c t g t c a g a c c a a g t t t a c t c c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a c c a t t g a c a g t c t g g t t c a a a t g a g

10,380 a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t a a t t t a a a a g g a t c t a g g t g a a g a t t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a t t a a a t t t t c c t a g a t c c a c t t c t a

10,440 c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c g t g a g t t t t c g t t c c a c t g a g c g t c g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g c a c t c a a a a g c a a g g t g a c t c g c a g

10,500 ori a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g a t c c t t t t t t t c t g c g c g t a a t c t g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c t a g g a a a a a a a g a c g c g c a t t a g a c

10,560 ori c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a

10,620 ori a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a

10,680 ori t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a

10,740 ori c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c

10,800 ori g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g

10,860 ori g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t

10,920 ori g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c

10,980 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t

11,040 ori t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c

11,100 ori g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c c t t t t t a c g g t t c c t g g c c t t t t g c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c g g a a a a a t g c c a a g g a c c g g a a a a c

11,160 c t g g c c t t g a a g c t g t c c c t g a t g g t c g t c a t c t a c c t g c c t g g a c a g c a t g g c c t g c a a g a c c g g a a c t t c g a c a g g g a c t a c c a g c a g t a g a t g g a c g g a c c t g t c g t a c c g g a c g t t

11,220 c g c g g g c a t c c c g a t g c c g c c g g a a g c g a g a a g a a t c a t a a t g g g g a a g g c c a t c c a g c c g c g c c c g t a g g g c t a c g g c g g c c t t c g c t c t t c t t a g t a t t a c c c c t t c c g g t a g g t c g g

3ʹ 5ʹ 11,228 t c g c g t c g a g c g c a g c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.