Addgene mem-mCherry-pTRIP Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: mem-mCherry-pTRIP Sequencing Result

Map View

SV40pA-R (12,030 .. 12,049) HpaI (11,992) SV40 NLS ApaI (11,273) PspOMI (11,269) PshAI (11,034) AvrII (10,204) SfiI (10,157) pBABE 3' (9885 .. 9905) L4440 (9798 .. 9815) pBR322ori-F (9545 .. 9564) FspI (8589) PvuI (8443) Amp-R (8243 .. 8262) pBRforEco (7834 .. 7852) pGEX 3' (7774 .. 7796) pRS-marker (7655 .. 7674) pBRrevBam (7422 .. 7441) PacI (6186) EBV-rev (12,084 .. 12,103) PmlI (290) NruI * (833) NotI (1144) KflI (1930) EcoRI (2024) NdeI (2403) SnaBI (2509) CMV promoter CMV-F (2688 .. 2708) LNCX (2734 .. 2758) PmeI (2829) BspDI * - ClaI * (2908) NheI (2913) BmtI (2917) mCherry-R (3077 .. 3095) SbfI (3287) mCherry-F (3345 .. 3364) XcmI (3618) BsrGI (3632) PaeR7I - XhoI (3653) TspMI - XmaI (3661) SmaI (3663) BamHI (3669) BclI * (3691) MSCV-rev (3742 .. 3765) SpeI (3952) BlpI (3980) mPGK-F (4139 .. 4158) Puro-R (4227 .. 4246) PasI (4276) BsiWI (4283) RsrII (4343) Puro-F (4723 .. 4743) WPRE-R (4893 .. 4913) Acc65I (5564) KpnI (5568) mem-mCherry-pTRIP 12,294 bp

Sequence View

5ʹ 3ʹ 60 3' LTR t g g a a g g g c t a a t t c a c t c c c a a a g a a g a c a a g a t a t c c t t g a t c t g t g g a t c t a c c a c a a c c t t c c c g a t t a a g t g a g g g t t t c t t c t g t t c t a t a g g a a c t a g a c a c c t a g a t g g t g t

120 3' LTR c a c a a g g c t a c t t c c c t g a t t a g c a g a a c t a c a c a c c a g g g c c a g g g g t c a g a t a t c c a c g t g t t c c g a t g a a g g g a c t a a t c g t c t t g a t g t g t g g t c c c g g t c c c c a g t c t a t a g g t g

180 3' LTR t g a c c t t t g g a t g g t g c t a c a a g c t a g t a c c a g t t g a g c c a g a t a a g g t a g a a g a g g c c a a c t g g a a a c c t a c c a c g a t g t t c g a t c a t g g t c a a c t c g g t c t a t t c c a t c t t c t c c g g t

240 3' LTR a t a a a g g a g a g a a c a c c a g c t t g t t a c a c c c t g t g a g c c t g c a t g g g a t g g a t g a c c c g g t a t t t c c t c t c t t g t g g t c g a a c a a t g t g g g a c a c t c g g a c g t a c c c t a c c t a c t g g g c c

PmlI 300 3' LTR a g a g a g a a g t g t t a g a g t g g a g g t t t g a c a g c c g c c t a g c a t t t c a t c a c g t g g c c c g a g t c t c t c t t c a c a a t c t c a c c t c c a a a c t g t c g g c g g a t c g t a a a g t a g t g c a c c g g g c t c

360 3' LTR a g c t g c a t c c g g a g t a c t t c a a g a a c t g c t g a t a t c g a g c t t g c t a c a a g g g a c t t t c c g t c g a c g t a g g c c t c a t g a a g t t c t t g a c g a c t a t a g c t c g a a c g a t g t t c c c t g a a a g g c

420 3' LTR c t g g g g a c t t t c c a g g g a g g c g t g g c c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t g a c c c c t g a a a g g t c c c t c c g c a c c g g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a

480 3' LTR c c t g c a t a t a a g c a g c t g c t t t t t g c c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g g a c g t a t a t t c g t c g a c g a a a a a c g g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t

540 3' LTR g c c t g g g a g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t c g g a c c c t c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a

600 3' LTR t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g

660 3' LTR a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c c c g a a c a g g g a c t t g a a a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g g g c t t g t c c c t g a a c t t t c

720 HIV-1 Ψ c g a a a g g g a a a c c a g a g g a g c t c t c t c g a c g c a g g a c t c g g c t t g c t g a a g c g c g c a c g g g c t t t c c c t t t g g t c t c c t c g a g a g a g c t g c g t c c t g a g c c g a a c g a c t t c g c g c g t g c c

780 HIV-1 Ψ c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g a c t a g c g g a g g c t a g a g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c t g a t c g c c t c c g a t c t

NruI* 840 HIV-1 Ψ a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a a t t a g a t c g c g a t g g g t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t t a a t c t a g c g c t a c c c

900 a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a a a a c a t a t a g t a t g g g t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t t t t g t a t a t c a t a c c c

960 c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a g a a a c a t c a g a a g g c t g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t c t t t g t a g t c t t c c g a

1020 g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a t c a g a a g a a c t t a g a t c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t a g t c t t c t t g a a t c t a

1080 c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a t a g a g a t a a a a g a c a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c t a t c t c t a t t t t c t g t

1140 c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t a a g a c c a c c g c a c a g c g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a t t c t g g t g g c g t g t c g

NotI 1200 MRDNWRSE a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a c a a t t g g a g a a g t g a a t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t g t t a a c c t c t t c a c t t

1260 LYKYKVVKIEPLGVAPTKAK t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c a c c c a c c a a g g c a a a g a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g t g g g t g g t t c c g t t t c

1320 RRE RRVVQREKRAVGIGALFLGF a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t t t g t t c c t t g g g t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g a a a c a a g g a a c c c a a g

1380 RRE LGAAGSTMGAASMTLTVQAR t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t g a c g g t a c a g g c c a g a a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a c t g c c a t g t c c g g t c t

1440 RRE QLLSGIVQQQNNLLRAIEAQ c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g g g c t a t t g a g g c g c a a g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c c c g a t a a c t c c g c g t t

1500 RRE QHLLQLTVWGIKQLQARILA c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a g g c a a g a a t c c t g g c t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t c c g t t c t t a g g a c c g a

1560 RRE VERYLKDQQLLGIWGCSGKL g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g t t g c t c t g g a a a a c t c c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c a a c g a g a c c t t t t g a g

1620 ICTTAVPWNASWSNKSLEQI a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a t c t c t g g a a c a g a t t t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t t a g a g a c c t t g t c t a a

1680 WNHTTWMEWDREINNYTSLI t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t t a c a c a a g c t t a a t a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t a a t g t g t t c g a a t t a t

1740 1 5 KNEQELLE gp41 peptide HSLIEESQNQQEKNEQELLE c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a c a a g a a t t a t t g g a a g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t t g t t c t t a a t a a c c t t

1800 10 15 LDKWASL (in frame with gp41 peptide) WNWFNITNWLWYI gp41 peptide LDKWASLWNWFNITNWLWYI t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t t g g c t g t g g t a t a t a a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t a a c c g a c a c c a t a t a t

1860 (in frame with gp41 peptide) KLFIMIVGGLVGLRIVFAVL KLFIMIVGGLVGLRIVFAVL a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a g t t t t t g c t g t a c t t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a t c a a a a a c g a c a t g a a

1920 (in frame with gp41 peptide) SIVNRVRQGYSPLSFQTHLP SIVNRVRQGYSPLSFQTHLP t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a g a c c c a c c t c c c a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a a g t c t g g g t g g a g g g t

KflI 1980 (in frame with gp41 peptide) TPRGPDRPEGIEEEGGERDR TPRGPDRPEGIEEEGGERDR a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g t g g a g a g a g a g a c a g a t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c a c c t c t c t c t c t g t c t

EcoRI 2040 (in frame with gp41 peptide) DRSIRLVNGSRRYRRIHKWQ DRSIRLVNGSRRYRRIHKWQ g a c a g a t c c a t t c g a t t a g t g a a c g g a t c t c g a c g g t a t c g c c g a a t t c a c a a a t g g c a g c t g t c t a g g t a a g c t a a t c a c t t g c c t a g a g c t g c c a t a g c g g c t t a a g t g t t t a c c g t c

2100 (in frame with gp41 peptide) YSSTILKEKGGLGGTVQGKE cPPT/CTS YSSTILKEKGGLGGTVQGKE t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g g g t a c a g t g c a g g g g a a a g a a a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c c c a t g t c a c g t c c c c t t t c t t

2160 * cPPT/CTS * t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t t a c a a a a a c a a a t t a c a a a a a a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a a t g t t t t t g t t t a a t g t t t t t

2220 cPPT/CTS t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c c a c t t t g g g g c g a t a a g c t t g a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g g t g a a a c c c c g c t a t t c g a a c

2280 CMV enhancer g g a g t t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c t c a a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g

2340 CMV enhancer c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t

2400 CMV enhancer t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t

NdeI 2460 CMV enhancer t c a t a t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a a g t a t a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t

SnaBI 2520 CMV enhancer t g c c c a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a

2580 CMV enhancer CMV promoter c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t

2640 CMV promoter c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t

2700 CMV promoter C G C A A A T G G G C G G CMV-F a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c

2760 CMV promoter T A G G C G T G CMV-F A G C T C G T T T A G T G A A C C G T C A G A T C LNCX t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g

2820 c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a c c g a c t c t a g a g g c c g g c g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t g g c t g a g a t c t c c g g c c g

PmeI 2880 c g a t a g t t t a a a c g g c c g g c c a t g c t g t g c t g t a t g a g a a g a a c c a a a c a g g t t g a a a a g g c t a t c a a a t t t g c c g g c c g g t a c g a c a c g a c a t a c t c t t c t t g g t t t g t c c a a c t t t t c

BmtINheIBspDI*ClaI* 2940 1 MVS mCherry MLALPVTMVS a a t g a t g a g g a c c a a a a g a t c g g c t g a t c g a t g c t a g c g c t a c c g g t c a c c a t g g t g a g c t t a c t a c t c c t g g t t t t c t a g c c g a c t a g c t a c g a t c g c g a t g g c c a g t g g t a c c a c t c g

3000 5 10 15 20 KGEEDNMAIIKEFMRFKVHM mCherry KGEEDNMAIIKEFMRFKVHM a a g g g c g a g g a g g a t a a c a t g g c c a t c a t c a a g g a g t t c a t g c g c t t c a a g g t g c a c a t g t t c c c g c t c c t c c t a t t g t a c c g g t a g t a g t t c c t c a a g t a c g c g a a g t t c c a c g t g t a c

3060 25 30 35 40 EGSVNGHEFEIEGEGEGRPY mCherry EGSVNGHEFEIEGEGEGRPY g a g g g c t c c g t g a a c g g c c a c g a g t t c g a g a t c g a g g g c g a g g g c g a g g g c c g c c c c t a c c t c c c g a g g c a c t t g c c g g t g c t c a a g c t c t a g c t c c c g c t c c c g c t c c c g g c g g g g a t g

3120 45 50 55 60 EGTQTAKLKVTKGGPLPFAW mCherry G G T T C G A C T T C C A C T G G T T mCherry-R EGTQTAKLKVTKGGPLPFAW g a g g g c a c c c a g a c c g c c a a g c t g a a g g t g a c c a a g g g t g g c c c c c t g c c c t t c g c c t g g c t c c c g t g g g t c t g g c g g t t c g a c t t c c a c t g g t t c c c a c c g g g g g a c g g g a a g c g g a c c

3180 65 70 75 80 DILSPQFMYGSKAYVKHPAD mCherry DILSPQFMYGSKAYVKHPAD g a c a t c c t g t c c c c t c a g t t c a t g t a c g g c t c c a a g g c c t a c g t g a a g c a c c c c g c c g a c c t g t a g g a c a g g g g a g t c a a g t a c a t g c c g a g g t t c c g g a t g c a c t t c g t g g g g c g g c t g

3240 85 90 95 100 IPDYLKLSFPEGFKWERVMN mCherry IPDYLKLSFPEGFKWERVMN a t c c c c g a c t a c t t g a a g c t g t c c t t c c c c g a g g g c t t c a a g t g g g a g c g c g t g a t g a a c t a g g g g c t g a t g a a c t t c g a c a g g a a g g g g c t c c c g a a g t t c a c c c t c g c g c a c t a c t t g

SbfI 3300 105 110 115 120 FEDGGVVTVTQDSSLQDGEF mCherry FEDGGVVTVTQDSSLQDGEF t t c g a g g a c g g c g g c g t g g t g a c c g t g a c c c a g g a c t c c t c c c t g c a g g a c g g c g a g t t c a a g c t c c t g c c g c c g c a c c a c t g g c a c t g g g t c c t g a g g a g g g a c g t c c t g c c g c t c a a g

3360 125 130 135 140 IYKVKLRGTNFPSDGPVMQK mCherry C C C C G T A A T G C A G A A G mCherry-F IYKVKLRGTNFPSDGPVMQK a t c t a c a a g g t g a a g c t g c g c g g c a c c a a c t t c c c c t c c g a c g g c c c c g t a a t g c a g a a g t a g a t g t t c c a c t t c g a c g c g c c g t g g t t g a a g g g g a g g c t g c c g g g g c a t t a c g t c t t c

3420 145 150 155 160 KTMGWEASSERMYPEDGALK mCherry A A G A mCherry-F KTMGWEASSERMYPEDGALK a a g a c c a t g g g c t g g g a g g c c t c c t c c g a g c g g a t g t a c c c c g a g g a c g g c g c c c t g a a g t t c t g g t a c c c g a c c c t c c g g a g g a g g c t c g c c t a c a t g g g g c t c c t g c c g c g g g a c t t c

3480 165 170 175 180 GEIKQRLKLKDGGHYDAEVK mCherry GEIKQRLKLKDGGHYDAEVK g g c g a g a t c a a g c a g a g g c t g a a g c t g a a g g a c g g c g g c c a c t a c g a c g c t g a g g t c a a g c c g c t c t a g t t c g t c t c c g a c t t c g a c t t c c t g c c g c c g g t g a t g c t g c g a c t c c a g t t c

3540 185 190 195 200 TTYKAKKPVQLPGAYNVNIK mCherry TTYKAKKPVQLPGAYNVNIK a c c a c c t a c a a g g c c a a g a a g c c c g t g c a g c t g c c c g g c g c c t a c a a c g t c a a c a t c a a g t g g t g g a t g t t c c g g t t c t t c g g g c a c g t c g a c g g g c c g c g g a t g t t g c a g t t g t a g t t c

3600 205 210 215 220 LDITSHNEDYTIVEQYERAE mCherry LDITSHNEDYTIVEQYERAE t t g g a c a t c a c c t c c c a c a a c g a g g a c t a c a c c a t c g t g g a a c a g t a c g a a c g c g c c g a g a a c c t g t a g t g g a g g g t g t t g c t c c t g a t g t g g t a g c a c c t t g t c a t g c t t g c g c g g c t c

PaeR7IXhoIBsrGIXcmI 3660 225 230 235 GRHSTGGMDELYK (in frame with mCherry) SGLRSRE mCherry GRHSTGGMDELYKSGLRSRE g g c c g c c a c t c c a c c g g c g g c a t g g a c g a g c t g t a c a a g t c c g g a c t c a g a t c t c g a g a a c c g g c g g t g a g g t g g c c g c c g t a c c t g c t c g a c a t g t t c a g g c c t g a g t c t a g a g c t c t t

BclI*BamHITspMIXmaISmaI 3720 (in frame with mCherry) PGKDPPDLDN* PGK promoter PGKDPPDLDN* c c c g g g a a g g a t c c a c c g g a t c t a g a t a a c t g a t c a t a a t t c t a c c g g g t a g g g g a g g c g g g g c c c t t c c t a g g t g g c c t a g a t c t a t t g a c t a g t a t t a a g a t g g c c c a t c c c c t c c g c

3780 PGK promoter C G T A C G C G A A A T C G T C G G G G C G A C MSCV-rev c t t t t c c c a a g g c a g t c t g g a g c a t g c g c t t t a g c a g c c c c g c t g g g c a c t t g g c g c t a c g a a a a g g g t t c c g t c a g a c c t c g t a c g c g a a a t c g t c g g g g c g a c c c g t g a a c c g c g a t g

3840 PGK promoter a c a a g t g g c c t c t g g c c t c g c a c a c a t t c c a c a t c c a c c g g t a g g c g c c a a c c g g c t c c g t g t t c a c c g g a g a c c g g a g c g t g t g t a a g g t g t a g g t g g c c a t c c g c g g t t g g c c g a g g c

3900 PGK promoter t t c t t t g g t g g c c c c t t c g c g c c a c c t t c t a c t c c t c c c c t a g t c a g g a a g t t c c c c c c c a a g a a a c c a c c g g g g a a g c g c g g t g g a a g a t g a g g a g g g g a t c a g t c c t t c a a g g g g g g g

SpeI 3960 PGK promoter g c c c c g c a g c t c g c g t c g t g c a g g a c g t g a c a a a t g g a a g t a g c a c g t c t c a c t a g t c t c c g g g g c g t c g a g c g c a g c a c g t c c t g c a c t g t t t a c c t t c a t c g t g c a g a g t g a t c a g a g

BlpI 4020 PGK promoter MEAGRPLGQRP g t g c a g a t g g a c a g c a c c g c t g a g c a a t g g a a g c g g g t a g g c c t t t g g g g c a g c g g c c a a c a c g t c t a c c t g t c g t g g c g a c t c g t t a c c t t c g c c c a t c c g g a a a c c c c g t c g c c g g t t

4080 PGK promoter IAALLLRFLGSEAGKGWVRG t a g c a g c t t t g c t c c t t c g c t t t c t g g g c t c a g a g g c t g g g a a g g g g t g g g t c c g g g g g c a t c g t c g a a a c g a g g a a g c g a a a g a c c c g a g t c t c c g a c c c t t c c c c a c c c a g g c c c c c g

4140 PGK promoter C A mPGK-F RAQGRAQGRGGRPKVLRRPG g g g c t c a g g g g c g g g c t c a g g g g c g g g g c g g g c g c c c g a a g g t c c t c c g g a g g c c c g g c a c c c g a g t c c c c g c c c g a g t c c c c g c c c c g c c c g c g g g c t t c c a g g a g g c c t c c g g g c c g t

4200 PGK promoter T T C T G C A C G C T T C A A A A G mPGK-F ILHASKAHVCRAVLLFLISG t t c t g c a c g c t t c a a a a g c g c a c g t c t g c c g c g c t g t t c t c c t c t t c c t c a t c t c c g g g c a a g a c g t g c g a a g t t t t c g c g t g c a g a c g g c g c g a c a a g a g g a g a a g g a g t a g a g g c c c g

4260 1 5 10 MTEYKPTVRLA PGK promoter PuroR T A C T G G C T C A T G T T C G G G T G Puro-R PFDLQPKLTMTEYKPTVRLA c t t t c g a c c t g c a g c c c a a g c t t a c c a t g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a g a a a g c t g g a c g t c g g g t t c g a a t g g t a c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t

BsiWIPasI 4320 15 20 25 30 TRDDVPRAVRTLAAAFADYP PuroR TRDDVPRAVRTLAAAFADYP c c c g c g a c g a c g t c c c c a g g g c c g t a c g c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g g g g c g c t g c t g c a g g g g t c c c g g c a t g c g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c

RsrII 4380 35 40 45 50 ATRHTVDPDRHIERVTELQE PuroR ATRHTVDPDRHIERVTELQE c c a c g c g c c a c a c c g t c g a t c c g g a c c g c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c g g t g c g c g g t g t g g c a g c t a g g c c t g g c g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g

4440 55 60 65 70 LFLTRVGLDIGKVWVADDGA PuroR LFLTRVGLDIGKVWVADDGA t c t t c c t c a c g c g c g t c g g g c t c g a c a t c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g a g a a g g a g t g c g c g c a g c c c g a g c t g t a g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c

4500 75 80 85 90 AVAVWTTPESVEAGAVFAEI PuroR AVAVWTTPESVEAGAVFAEI c g g t g g c g g t c t g g a c c a c g c c g g a g a g c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c a c c g c c a g a c c t g g t g c g g c c t c t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c

4560 95 100 105 110 GPRMAELSGSRLAAQQQMEG PuroR GPRMAELSGSRLAAQQQMEG g c c c g c g c a t g g c c g a g t t g a g c g g t t c c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c c g g g c g c g t a c c g g c t c a a c t c g c c a a g g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g

4620 115 120 125 130 LLAPHRPKEPAWFLATVGVS PuroR LLAPHRPKEPAWFLATVGVS t c c t g g c g c c g c a c c g g c c c a a g g a g c c c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c a g g a c c g c g g c g t g g c c g g g t t c c t c g g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g

4680 135 140 145 150 PDHQGKGLGSAVVLPGVEAA PuroR PDHQGKGLGSAVVLPGVEAA c c g a c c a c c a g g g c a a g g g t c t g g g c a g c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c

4740 155 160 165 170 ERAGVPAFLETSAPRNLPFY PuroR G C A A C C T C C C C T T C T A C G Puro-F ERAGVPAFLETSAPRNLPFY a g c g c g c c g g g g t g c c c g c c t t c c t g g a g a c c t c c g c g c c c c g c a a c c t c c c c t t c t a c g t c g c g c g g c c c c a c g g g c g g a a g g a c c t c t g g a g g c g c g g g g c g t t g g a g g g g a a g a t g c

4800 175 180 185 190 ERLGFTVTADVEVPEGPRTW PuroR A G C Puro-F ERLGFTVTADVEVPEGPRTW a g c g g c t c g g c t t c a c c g t c a c c g c c g a c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t t c g c c g a g c c g a a g t g g c a g t g g c g g c t g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a

4860 195 200 CMTRKPGA* PuroR WPRE CMTRKPGA* g c a t g a c c c g c a a g c c c g g t g c c t g a c c g c g t c t g g a a c a a t c a a c c t c t g g a t t a c a a a c g t a c t g g g c g t t c g g g c c a c g g a c t g g c g c a g a c c t t g t t a g t t g g a g a c c t a a t g t t t

4920 WPRE A C A A C G A G G A A A A T G C G A T A C WPRE-R a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g t t g c t c c t t t t a c g c t a t g t g g a t a c t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c a a c g a g g a a a a t g c g a t a c a c c t a t g

4980 WPRE (in frame with Factor Xa site) *HRQIMSNSGTHSENEGG MPLYHAIASRMAFIFSS g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t c c c g t a t g g c t t t c a t t t t c t c c t c c c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a g g g c a t a c c g a a a g t a a a a g a g g a g g

5040 WPRE (in frame with Factor Xa site) QIFGPQQRKILLQPGNDPLT LYKSWLLSLYEELWPVVRQR t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g a g t t g t g g c c c g t t g t c a g g c a a c g t a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c t c a a c a c c g g g c a a c a g t c c g t t g c a

5100 WPRE (in frame with Factor Xa site) AHHASHKSVCGGSTPANGGG GVVCTVFADATPTGWGIATT g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c c c a c t g g t t g g g g c a t t g c c a c c a c c c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g g g t g a c c a a c c c c g t a a c g g t g g t g g

5160 WPRE (in frame with Factor Xa site) TLEKGPSESEGERNGRRFED CQLLSGTFAFPLPIATAELI t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c t c c c t a t t g c c a c g g c g g a a c t c a t c a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g a g g g a t a a c g g t g c c g c c t t g a g t a g

5220 WPRE (in frame with Factor Xa site) GGAKGAAPCPSPQQASVIGH AACLARCWTGARLLGTDNSV g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c g g c t g t t g g g c a c t g a c a a t t c c g t g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g c c g a c a a c c c g t g a c t g t t a a g g c a c

5280 WPRE (in frame with Factor Xa site) HQRPLQRGKWPQEGTNGGPN VLSGKLTSFPWLLACVATWI g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c t g c t c g c c t g t g t t g c c a c c t g g a t t c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g a c g a g c g g a c a c a a c g g t g g a c c t a a

5340 WPRE (in frame with Factor Xa site) QAPRGEAVDR 1 RGEI Factor Xa site LRGTSFCYVPSALNPADLPS c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g c c c t c a a t c c a g c g g a c c t t c c t t c c g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c g g g a g t t a g g t c g c c t g g a a g g a a g g

5400 WPRE RGLLPALRPLPRLRLRPQTS c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g t c t t c g c c t t c g c c c t c a g a c g a g t g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g c a g a a g c g g a a g c g g g a g t c t g c t c a

5460 WPRE RISLWAASPPGINSAVET* c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g g a a t t a a t t c t g c a g t c g a g a c c t a g a a a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c c t t a a t t a a g a c g t c a g c t c t g g a t c t t t

5520 a a c a t g g a g c a a t c a c a a g t a g c a a t a c a g c a g c t a c c a a t g c t g a t t g t g c c t g g c t a g t t g t a c c t c g t t a g t g t t c a t c g t t a t g t c g t c g a t g g t t a c g a c t a a c a c g g a c c g a t c

Acc65IKpnI 5580 a a g c a c a a g a g g a g g a g g a g g t g g g t t t t c c a g t c a c a c c t c a g g t a c c t t t a a g a c c a a t t c g t g t t c t c c t c c t c c t c c a c c c a a a a g g t c a g t g t g g a g t c c a t g g a a a t t c t g g t t

5640 t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c

5700 g g c t a a t t c a c t c c c a a c g a a g a c a a a a t c g t c g a g a g a t g c t g c a t a t a a g c a g c t g c t c c g a t t a a g t g a g g g t t g c t t c t g t t t t a g c a g c t c t c t a c g a c g t a t a t t c g t c g a c g a

5760 5' LTR (truncated) t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t

5820 5' LTR (truncated) a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c

5880 5' LTR (truncated) t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c

5940 5' LTR (truncated) g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g c a a a c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c g t t t

6000 g a a a t g a a t a t c a g a g a g t g a g a g g c c t t g a c a t t a t a a t a g a t t t a g c a g g a a t t g a a c c t t t a c t t a t a g t c t c t c a c t c t c c g g a a c t g t a a t a t t a t c t a a a t c g t c c t t a a c t t g

6060 t a g g a g t g g a g c a c a c a g g c a a a g c t g c a g a a g t a c t t g g a a g a a g c c a c c a g a g a t a c t a t c c t c a c c t c g t g t g t c c g t t t c g a c g t c t t c a t g a a c c t t c t t c g g t g g t c t c t a t g a

6120 c a c g a t t c t g c a c a t a c c t g g c t a a t c c c a g a t c c t a a g g a t t a c a t t a a g t t t a c t a a c g t g c t a a g a c g t g t a t g g a c c g a t t a g g g t c t a g g a t t c c t a a t g t a a t t c a a a t g a t t g

6180 a t t t a t a t a a t g a t t t a t a g t t t a a a g t a t a a a c t t a t c t a a t t t a c t a t t c t g a c a g a t t a a a t a t a t t a c t a a a t a t c a a a t t t c a t a t t t g a a t a g a t t a a a t g a t a a g a c t g t c t a

PacI 6240 a t t a a t t a a t c c t c a a a t a t c a t a a g a g a t g a t t a c t a t t a t c c c c a t t t a a c a c a a g a g t a a t t a a t t a g g a g t t t a t a g t a t t c t c t a c t a a t g a t a a t a g g g g t a a a t t g t g t t c t c

6300 g a a a c t g a g a g g g a a a g a t g t t g a a g t a a t t t t c c c a c a a t t a c a g c a t c c g t t a g t t a c c t t t g a c t c t c c c t t t c t a c a a c t t c a t t a a a a g g g t g t t a a t g t c g t a g g c a a t c a a t g

6360 g a c t c t a t g a t c t t c t g a c a c a a a t t c c a t t t a c t c c t c a c c c t a t g a c t c a g t c g a a t a c t g a g a t a c t a g a a g a c t g t g t t t a a g g t a a a t g a g g a g t g g g a t a c t g a g t c a g c t t a t

6420 t a t c a a a g t t a t g g a c a t t a t g c t a a g t a a c a a a t t a c c c t t t t a t a t a g t a a a t a c t g a a t a g t t t c a a t a c c t g t a a t a c g a t t c a t t g t t t a a t g g g a a a a t a t a t c a t t t a t g a c t

6480 g t a g a t t g a g a g a a g a a a t t g t t t g c a a a c c t g a a t a g c t t c a a g a a g a a g a g a a g t g a g c a t c t a a c t c t c t t c t t t a a c a a a c g t t t g g a c t t a t c g a a g t t c t t c t t c t c t t c a c t c

6540 g a t a a g a a t a a c a g t t g t c a t t t a a c a a g t t t t a a c a a g t a a c t t g g t t a g a a a g g g a t t c t a t t c t t a t t g t c a a c a g t a a a t t g t t c a a a a t t g t t c a t t g a a c c a a t c t t t c c c t a a

6600 c a a a t g c a t a a a g c a a g g g a t a a a t t t t t c t g g c a a c a a g a c t a t a c a a t a t a a c c t t a a g t t t a c g t a t t t c g t t c c c t a t t t a a a a a g a c c g t t g t t c t g a t a t g t t a t a t t g g a a t t

6660 a t a t g a c t t c a a a t a a t t g t t g g a a c t t g a t a a a a c t a a t t a a a t a t t a t t g a a g a t t a t t a t a c t g a a g t t t a t t a a c a a c c t t g a a c t a t t t t g a t t a a t t t a t a a t a a c t t c t a a t a

6720 c a a t a t t a t a a a t g t a a t t t a c t t t t a a a a a g g g a a c a t a g a a a t g t g t a t c a t t a g a g t g t t a t a a t a t t t a c a t t a a a t g a a a a t t t t t c c c t t g t a t c t t t a c a c a t a g t a a t c t c a

6780 a g a a a a c a a t c c t t a t t a t c a c a a t t t g t c a a a a c a a g t t t g t t a t t a a c a c a a g t a g a a t c t t t t g t t a g g a a t a a t a g t g t t a a a c a g t t t t g t t c a a a c a a t a a t t g t g t t c a t c t t

6840 t a c t g c a t t c a a t t a a g t t g a c t g c a g a t t t t g t g t t t t g t t a a a a t t a g a a a g a g a t a a a t g a c g t a a g t t a a t t c a a c t g a c g t c t a a a a c a c a a a a c a a t t t t a a t c t t t c t c t a t t

6900 c a a c a a t t t g a a t t a t t g a a a g t a a c a t g t a a a t a g t t c t a c a t a c g t t c t t t t g a c a t c g t t g t t a a a c t t a a t a a c t t t c a t t g t a c a t t t a t c a a g a t g t a t g c a a g a a a a c t g t a g

6960 t t g t t c a a t c a t t g a t c g a a g t t c t t t a t c t t g g a a g a a t t t g t t c c a a a g a c t c t g a a a a a c a a g t t a g t a a c t a g c t t c a a g a a a t a g a a c c t t c t t a a a c a a g g t t t c t g a g a c t t t

7020 t a a g g a a a a c a a t c t a t t a t a t a g t c t c a c a c c t t t g t t t t a c t t t t a g t g a t t t c a a t t a t t c c t t t t g t t a g a t a a t a t a t c a g a g t g t g g a a a c a a a a t g a a a a t c a c t a a a g t t a a

7080 t a a t a a t g t a a a t g g t t a a a a t t t a t t c t t c t c t g a g a t c a t t t c a c a t t g c a g a t a g a a a t t a t t a c a t t t a c c a a t t t t a a a t a a g a a g a g a c t c t a g t a a a g t g t a a c g t c t a t c t t

7140 a a c c t g a g a c t g g g g t a a t t t t t a t t a a a a t c t a a t t t a a t c t c a g a a a c a c a t c t t t a t t t g g a c t c t g a c c c c a t t a a a a a t a a t t t t a g a t t a a a t t a g a g t c t t t g t g t a g a a a t a

7200 t c t a a c a t c a a t t t t t c c a g t t t g a t a t t a t c a t a t a a a g t c a g c c t t c c t c a t c t g c a g a g a t t g t a g t t a a a a a g g t c a a a c t a t a a t a g t a t a t t t c a g t c g g a a g g a g t a g a c g t c

7260 g t t c c a c a a c a a a a a t c c a a c c a a c t g t g g a t c a a a a a t a t t g g g a a a a a a t t a a a a a t a c a a g g t g t t g t t t t t a g g t t g g t t g a c a c c t a g t t t t t a t a a c c c t t t t t t a a t t t t t a t

7320 g c a a t a c a a c a a t a a a a a a a t a c a a a t c a g a a a a a c a g c a c a g t a t a a c a a c t t t a t t t a c g t t a t g t t g t t a t t t t t t t a t g t t t a g t c t t t t t g t c g t g t c a t a t t g t t g a a a t a a a t

7380 g c a t t t a c a a t c t a t t a g g t a t t a t a a g t a a t c t a g c c a g a t c c t c t a c g c c g g a c g c a t c g t a a a t g t t a g a t a a t c c a t a a t a t t c a t t a g a t c g g t c t a g g a g a t g c g g c c t g c g t a

7440 G G A T A T A G C G G C T G T A G T G pBRrevBam c g t g g c c g g c a t c a c c g g c g c c a c a g g t g c g g t t g c t g g c g c c t a t a t c g c c g a c a t c a c g c a c c g g c c g t a g t g g c c g c g g t g t c c a c g c c a a c g a c c g c g g a t a t a g c g g c t g t a g t g

7500 G pBRrevBam c g a t g g g g a a g a t c g g g c t c g c c a c t t c g g g c t c a t g a g c g c t t g t t t c g g c g t g g g t a t g c t a c c c c t t c t a g c c c g a g c g g t g a a g c c c g a g t a c t c g c g a a c a a a g c c g c a c c c a t a

7560 g g t g g c a g g c c c c g t g g c c g g g g g a c t g t t g g g c g c c a t c t c c t t g c a t g c a c c a t t c c t c c a c c g t c c g g g g c a c c g g c c c c c t g a c a a c c c g c g g t a g a g g a a c g t a c g t g g t a a g g a

7620 t g c g g c g g c g g t g c t c a a c g g c c t c a a c c t a c t a c t g g g c t g c t t c c t a a t g c a g g a g t c a c g c c g c c g c c a c g a g t t g c c g g a g t t g g a t g a t g a c c c g a c g a a g g a t t a c g t c c t c a g

7680 A T G T T A G A C G A G A C T A C G G C pRS-marker g c a t a a g g g a g a g c g t c g a a t g g t g c a c t c t c a g t a c a a t c t g c t c t g a t g c c g c a t a g t c g t a t t c c c t c t c g c a g c t t a c c a c g t g a g a g t c a t g t t a g a c g a g a c t a c g g c g t a t c a

7740 t a a g c c a g c c c c g a c a c c c g c c a a c a c c c g c t g a c g c g c c c t g a c g g g c t t g t c t g c t c c a t t c g g t c g g g g c t g t g g g c g g t t g t g g g c g a c t g c g c g g g a c t g c c c g a a c a g a c g a g g

7800 C C G G G A G C T G C A T G T G T C A G A G G pGEX 3' c g g c a t c c g c t t a c a g a c a a g c t g t g a c c g t c t c c g g g a g c t g c a t g t g t c a g a g g t t t t g c c g t a g g c g a a t g t c t g t t c g a c a c t g g c a g a g g c c c t c g a c g t a c a c a g t c t c c a a a a

7860 C G G A G C A C T A T G C G G A T A A pBRforEco c a c c g t c a t c a c c g a a a c g c g c g a g a c g a a a g g g c c t c g t g a t a c g c c t a t t t t t a t a g g g t g g c a g t a g t g g c t t t g c g c g c t c t g c t t t c c c g g a g c a c t a t g c g g a t a a a a a t a t c c

7920 AmpR promoter t t a a t g t c a t g a t a a t a a t g g t t t c t t a g a c g t c a g g t g g c a c t t t t c g g g g a a a t g t g c a a t t a c a g t a c t a t t a t t a c c a a a g a a t c t g c a g t c c a c c g t g a a a a g c c c c t t t a c a c g

7980 AmpR promoter g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g

8040 1 5 signal sequence MSIQH AmpR promoter AmpR MSIQH a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a

8100 10 15 20 signal sequence FRVALIPFFAAFCLPVFA 25 HP AmpR FRVALIPFFAAFCLPVFAHP t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c

8160 30 35 40 45 ETLVKVKDAEDQLGARVGYI AmpR ETLVKVKDAEDQLGARVGYI a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c

8220 50 55 60 65 ELDLNSGKILESFRPEERFP AmpR ELDLNSGKILESFRPEERFP a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t

8280 70 75 80 85 MMSTFKVLLCGAVLSRIDAG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MMSTFKVLLCGAVLSRIDAG t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g

8340 90 95 100 105 QEQLGRRIHYSQNDLVEYSP AmpR QEQLGRRIHYSQNDLVEYSP a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c

8400 110 115 120 125 VTEKHLTDGMTVRELCSAAI AmpR VTEKHLTDGMTVRELCSAAI t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t

PvuI 8460 130 135 140 145 TMSDNTAANLLLTTIGGPKE AmpR TMSDNTAANLLLTTIGGPKE c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g

8520 150 155 160 165 LTAFLHNMGDHVTRLDRWEP AmpR LTAFLHNMGDHVTRLDRWEP t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c

8580 170 175 180 185 ELNEAIPNDERDTTMPVAMA AmpR ELNEAIPNDERDTTMPVAMA a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t

FspI 8640 190 195 200 205 TTLRKLLTGELLTLASRQQL AmpR TTLRKLLTGELLTLASRQQL c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t

8700 210 215 220 225 IDWMEADKVAGPLLRSALPA AmpR IDWMEADKVAGPLLRSALPA t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c

8760 230 235 240 245 GWFIADKSGAGERGSRGIIA AmpR GWFIADKSGAGERGSRGIIA g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c

8820 250 255 260 265 ALGPDGKPSRIVVIYTTGSQ AmpR ALGPDGKPSRIVVIYTTGSQ c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c

8880 270 275 280 285 ATMDERNRQIAEIGASLIKH AmpR ATMDERNRQIAEIGASLIKH c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a

8940 W* AmpR W* g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a

9000 a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g

9060 ori g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

9120 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

9180 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

9240 ori g a g c g c a g a t a c c a a a t a c t g t t c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a a g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

9300 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

9360 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

9420 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

9480 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

9540 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

9600 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

9660 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c

9720 c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a

9780 c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t

9840 A G C G A G T C A G T G A G C G A G L4440 g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t

9900 SV40 promoter C C T T A C A C A C A G T C A A pBABE 3' a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g t g g a a t g t g t g t c a g t t t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c a c c t t a c a c a c a g t c a a

9960 SV40 promoter T C C C A pBABE 3' a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t

10,020 SV40 promoter t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c

10,080 SV40 promoter SV40 ori c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a

10,140 SV40 promoter SV40 ori a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g

SfiI 10,200 SV40 promoter SV40 ori a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c

AvrII 10,260 SV40 promoter SV40 ori a g g c c t a g g c t t t t g c a a a a a g c t t g g a c a c a a g a c a g g c t t g c g a g a t a t g t t t g a g a a t c c g g a t c c g a a a a c g t t t t t c g a a c c t g t g t t c t g t c c g a a c g c t c t a t a c a a a c t c t t

10,320 t a c c a c t t t a t c c c g c g t c a g g g a g a g g c a g t g c g t a a a a a g a c g c g g a c t c a t g t g a a a a t g g t g a a a t a g g g c g c a g t c c c t c t c c g t c a c g c a t t t t t c t g c g c c t g a g t a c a c t t t

10,380 t a c t g g t t t t t a g t g c g c c a g a t c t c t a t a a t c t c g c g c a a c c t a t t t t c c c c t c g a a c a a t g a c c a a a a a t c a c g c g g t c t a g a g a t a t t a g a g c g c g t t g g a t a a a a g g g g a g c t t g t

10,440 c t t t t t a a g c c g t a g a t a a a c a g g c t g g g a c a c t t c a c a t g a g c g a a a a a t a c a t c g t c a g a a a a a t t c g g c a t c t a t t t g t c c g a c c c t g t g a a g t g t a c t c g c t t t t t a t g t a g c a g t

10,500 c c t g g g a c a t g t t g c a g a t c c a t g c a c g t a a a c t c g c a a g c c g a c t g a t g c c t t c t g a a c g g a c c c t g t a c a a c g t c t a g g t a c g t g c a t t t g a g c g t t c g g c t g a c t a c g g a a g a c t t g

10,560 a a t g g a a a g g c a t t a t t g c c g t a a g c c g t g g c g g t c t g t a c c g g g t g c g t t a c t g g c g c g t t a c c t t t c c g t a a t a a c g g c a t t c g g c a c c g c c a g a c a t g g c c c a c g c a a t g a c c g c g c

10,620 t g a a c t g g g t a t t c g t c a t g t c g a t a c c g t t t g t a t t t c c a g c t a c g a t c a c g a c a a c c a a c t t g a c c c a t a a g c a g t a c a g c t a t g g c a a a c a t a a a g g t c g a t g c t a g t g c t g t t g g t

10,680 g c g c g a g c t t a a a g t g c t g a a a c g c g c a g a a g g c g a t g g c g a a g g c t t c a t c g t t a t t g a c g c g c t c g a a t t t c a c g a c t t t g c g c g t c t t c c g c t a c c g c t t c c g a a g t a g c a a t a a c t

10,740 t g a c c t g g t g g a t a c c g g t g g t a c t g c g g t t g c g a t t c g t g a a a t g t a t c c a a a a g c g c a a c t g g a c c a c c t a t g g c c a c c a t g a c g c c a a c g c t a a g c a c t t t a c a t a g g t t t t c g c g t

10,800 c t t t g t c a c c a t c t t c g c a a a a c c g g c t g g t c g t c c g c t g g t t g a t g a c t a t g t t g t t g a g a a a c a g t g g t a g a a g c g t t t t g g c c g a c c a g c a g g c g a c c a a c t a c t g a t a c a a c a a c t

10,860 t a t c c c g c a a g a t a c c t g g a t t g a a c a g c c g t g g g a t a t g g g c g t c g t a t t c g t c c c g c c a t a g g g c g t t c t a t g g a c c t a a c t t g t c g g c a c c c t a t a c c c g c a g c a t a a g c a g g g c g g

10,920 a a t c t c c g g t c g c t a a t c t t t t c a a c g c c t g g c a c t g c c g g g c g t t g t t c t t t t t a a c t t t t a g a g g c c a g c g a t t a g a a a a g t t g c g g a c c g t g a c g g c c c g c a a c a a g a a a a a t t g a a

10,980 c a g g c g g g t t a c a a t a g t t t c c a g t a a g t a t t c t g g a g g c t g c a t c c a t g a c a c a g g c a a g t c c g c c c a a t g t t a t c a a a g g t c a t t c a t a a g a c c t c c g a c g t a g g t a c t g t g t c c g t t

PshAI 11,040 a c c t g a g c g a a a c c c t g t t c a a a c c c c g c t t t a a a c a t c c t g a a a c c t c g a c g c t a g t c c t g g a c t c g c t t t g g g a c a a g t t t g g g g c g a a a t t t g t a g g a c t t t g g a g c t g c g a t c a g g

11,100 g c c g c t t t a a t c a c g g c g c a c a a c c g c c t g t g c a g t c g g c c c t t g a t g g t a a a a c c a t c c c g g c g a a a t t a g t g c c g c g t g t t g g c g g a c a c g t c a g c c g g g a a c t a c c a t t t t g g t a g g

11,160 c t c a c t g g t a t c g c a t g a t t a a c c g t c t g a t g t g g a t c t g g c g c g g c a t t g a c c c a c g c g g a g t g a c c a t a g c g t a c t a a t t g g c a g a c t a c a c c t a g a c c g c g c c g t a a c t g g g t g c g c

11,220 a a a t c c t c g a c g t c c a g g c a c g t a t t g t g a t g a g c g a t g c c g a a c g t a c c g a c g a t g a t t t t t a g g a g c t g c a g g t c c g t g c a t a a c a c t a c t c g c t a c g g c t t g c a t g g c t g c t a c t a a

PspOMIApaI 11,280 t a t a c g a t a c g g t g a t t g g c t a c c g t g g c g g c a a c t g g a t t t a t g a g t g g g c c c c g g a t c a t a t g c t a t g c c a c t a a c c g a t g g c a c c g c c g t t g a c c t a a a t a c t c a c c c g g g g c c t a g

11,340 t t t g t g a a g g a a c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c a a a c t a c c t a c a g a g a t t a a a c a c t t c c t t g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g t t t g a t g g a t g t c t c t a a

11,400 small t intron t a a a g c t c t a a g g t a a a t a t a a a a t t t t t a a g t g t a t a a t g t g t t a a a c t a c t g a t t c t a a t t t c g a g a t t c c a t t t a t a t t t t a a a a a t t c a c a t a t t a c a c a a t t t g a t g a c t a a g a t

11,460 small t intron a t t g t t t g t g t a t t t t a g a t t c c a a c c t a t g g a a c t g a t g a a t g g g a g c a g t g g t g g a a t t a a c a a a c a c a t a a a a t c t a a g g t t g g a t a c c t t g a c t a c t t a c c c t c g t c a c c a c c t t a

11,520 MPSSDDEA g c c t t t a a t g a g g a a a a c c t g t t t t g c t c a g a a g a a a t g c c a t c t a g t g a t g a t g a g g c t c g g a a a t t a c t c c t t t t g g a c a a a a c g a g t c t t c t t t a c g g t a g a t c a c t a c t a c t c c g a

11,580 130 PKKKRKV EDPK SV40 NLS TADSQHSTPPKKKRKVEDPK a c t g c t g a c t c t c a a c a t t c t a c t c c t c c a a a a a a g a a g a g a a a g g t a g a a g a c c c c a a g t g a c g a c t g a g a g t t g t a a g a t g a g g a g g t t t t t t c t t c t c t t t c c a t c t t c t g g g g t t c

11,640 (in frame with SV40 NLS) DFPSELLSFLSHAVFSNRTL DFPSELLSFLSHAVFSNRTL g a c t t t c c t t c a g a a t t g c t a a g t t t t t t g a g t c a t g c t g t g t t t a g t a a t a g a a c t c t t c t g a a a g g a a g t c t t a a c g a t t c a a a a a a c t c a g t a c g a c a c a a a t c a t t a t c t t g a g a a

11,700 (in frame with SV40 NLS) ACFAIYTTKEKAALLYKKIM ACFAIYTTKEKAALLYKKIM g c t t g c t t t g c t a t t t a c a c c a c a a a g g a a a a a g c t g c a c t g c t a t a c a a g a a a a t t a t g c g a a c g a a a c g a t a a a t g t g g t g t t t c c t t t t t c g a c g t g a c g a t a t g t t c t t t t a a t a c

11,760 (in frame with SV40 NLS) EKYSVTFISRHNSYNHNILF EKYSVTFISRHNSYNHNILF g a a a a a t a t t c t g t a a c c t t t a t a a g t a g g c a t a a c a g t t a t a a t c a t a a c a t a c t g t t t c t t t t t a t a a g a c a t t g g a a a t a t t c a t c c g t a t t g t c a a t a t t a g t a t t g t a t g a c a a a

11,820 (in frame with SV40 NLS) FLTPHRHRVSAINNYAQKLC FLTPHRHRVSAINNYAQKLC t t t c t t a c t c c a c a c a g g c a t a g a g t g t c t g c t a t t a a t a a c t a t g c t c a a a a a t t g t g t a a a g a a t g a g g t g t g t c c g t a t c t c a c a g a c g a t a a t t a t t g a t a c g a g t t t t t a a c a c a

11,880 (in frame with SV40 NLS) TFSFLICKGVNKEYLMYSAL TFSFLICKGVNKEYLMYSAL a c c t t t a g c t t t t t a a t t t g t a a a g g g g t t a a t a a g g a a t a t t t g a t g t a t a g t g c c t t g t g g a a a t c g a a a a a t t a a a c a t t t c c c c a a t t a t t c c t t a t a a a c t a c a t a t c a c g g a a c

11,940 (in frame with SV40 NLS) TRDHNQPYHICRGFTCFKKP TRDHNQPYHICRGFTCFKKP a c t a g a g a t c a t a a t c a g c c a t a c c a c a t t t g t a g a g g t t t t a c t t g c t t t a a a a a a c c t t g a t c t c t a g t a t t a g t c g g t a t g g t g t a a a c a t c t c c a a a a t g a a c g a a a t t t t t t g g a

HpaI 12,000 (in frame with SV40 NLS) PTPPPEPET* SV40 poly(A) signal PTPPPEPET* c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t g a a t g c a a t t g t t g t t g t t a a c t t g t t g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t a c t t a c g t t a a c a a c a a c a a t t g a a c a a

12,060 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g

12,120 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a

12,180 SV40 poly(A) signal c t g g a t c a a c t g g a t a a c t c a a g c t a a c c a a a a t c a t c c c a a a c t t c c c a c c c c a t a c c c g a c c t a g t t g a c c t a t t g a g t t c g a t t g g t t t t a g t a g g g t t t g a a g g g t g g g g t a t g g g

12,240 t a t t a c c a c t g c c a a t t a c c t g t g g t t t c a t t t a c t c t a a a c c t g t g a t t c c t c t g a a t t a t a a t g g t g a c g g t t a a t g g a c a c c a a a g t a a a t g a g a t t t g g a c a c t a a g g a g a c t t a a

3ʹ 5ʹ 12,294 a t t t t c a t t t t a a a g a a a t t g t a t t t g t t a a a t a t g t a c t a c a a a c t t a g t a g t t a a a a g t a a a a t t t c t t t a a c a t a a a c a a t t t a t a c a t g a t g t t t g a a t c a t c a

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.