Addgene pEPQD0CB0246 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pEPQD0CB0246 Sequencing Result


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NgoMIV (4264) SgrAI (4255) pBRrevBam (4213 .. 4232) AfeI (4173) pGEX 3' (3858 .. 3880) PfoI (3875) pBRforEco (3802 .. 3820) SspI (3646) XmnI (3441) Amp-R (3392 .. 3411) ScaI (3322) AhdI (2842) AlwNI (2365) NaeI (4266) BsrGI (177) SP6 (241 .. 258) EcoRV (333) SpeI (337) PaeR7I - XhoI (344) NdeI (490) BstAPI (491) M13/pUC Forward (670 .. 692) M13 Forward (684 .. 701) Acc65I (714) KpnI - TspMI - XmaI (718) SmaI (720) BamHI (723) XbaI (729) PstI - SbfI (745) lac operator PsiI (957) Bsu36I (965) NcoI - StyI (979) PshAI (1022) NsiI (1330) PmlI (1369) BsiWI - SnaBI (1376) BssHII (1433) BstBI (1495) PspOMI (1521) ApaI (1525) SacII (1586) CAP binding site BspQI - SapI (1833) PciI (1949) pBR322ori-F (2090 .. 2109) pEPQD0CB0246 4281 bp

Sequence View

60 g g c g t a g a g g a t c t g g c t a g c g a t g a c c c t g c t g a t t g g t t c g c t g a c c a t t t c c g g g t g c c g c a t c t c c t a g a c c g a t c g c t a c t g g g a c g a c t a a c c a a g c g a c t g g t a a a g g c c c a c

120 c g g g a c g g c g t t a c c a g a a a c t c a g a a g g t t c g t c c a a c c a a a c c g a c t c t g g c g g c a g t g c c c t g c c g c a a t g g t c t t t g a g t c t t c c a a g c a g g t t g g t t t g g c t g a g a c c g c c g t c a

BsrGI 180 t t a c g a g a g a g a t g a t a g g g t c t g c t t c a g t a a g c c a g a t g c t a c a c a a t t a g g c t t g t a a a t g c t c t c t c t a c t a t c c c a g a c g a a g t c a t t c g g t c t a c g a t g t g t t a a t c c g a a c a t

240 c a t a c t g t c g t t a g a a c g c g g c t a c a a t t a a t a c a t a a c c t t a t g t a t c a t a c a c a t a c g g t a t g a c a g c a a t c t t g c g c c g a t g t t a a t t a t g t a t t g g a a t a c a t a g t a t g t g t a t g c

300 SP6 promoter A T T T A G G T G A C A C T A T A G SP6 a t t t a g g t g a c a c t a t a g a a c t c a c c t a t c t c c c c a a c a c c t a a t a a c a t t c a a t c a c t c t a a a t c c a c t g t g a t a t c t t g a g t g g a t a g a g g g g t t g t g g a t t a t t g t a a g t t a g t g a g

PaeR7IXhoIEcoRVSpeI 360 t t t c c a c t a a c c a c c t a t c t a c a t c a c c a a g a t a t c a c t a g t t c t c g a g c t c c a t t g a g a a a a g g t g a t t g g t g g a t a g a t g t a g t g g t t c t a t a g t g a t c a a g a g c t c g a g g t a a c t c t

420 c c g t c a c a g c t t g t c t g t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g g g c a g t g t c g a a c a g a c a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c

480 105 100 *AADSCITSL lacZ α c g g g t g t t g g c g g g t g t c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g c c c a c a a c c g c c c a c a g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t

NdeIBstAPI 540 95 90 85 80 TCWIRHSIGCLHTLLFYRML lacZ α g a g t g c a c c a t a t g c g g t g t g a a a t a c c g c a c a g a t g c g t a a g g a g a a a a t a c c g c a t c a c t c a c g t g g t a t a c g c c a c a c t t t a t g g c g t g t c t a c g c a t t c c t c t t t t a t g g c g t a g t

600 75 70 65 60 RWEGNLSRLQQSPRDTRAEE lacZ α g g c g c c a t t c g c c a t t c a g g c t g c g c a a c t g t t g g g a a g g g c g a t c g g t g c g g g c c t c t t c c g c g g t a a g c g g t a a g t c c g a c g c g t t g a c a a c c c t t c c c g c t a g c c a c g c c c g g a g a a

660 55 50 45 40 SNRWSAFPPHAALRNLQTVG lacZ α c g c t a t t a c g c c a g c t g g c g a a a g g g g g a t g t g c t g c a a g g c g a t t a a g t t g g g t a a c g c g c g a t a a t g c g g t c g a c c g c t t t c c c c c t a c a c g a c g t t c c g c t a a t t c a a c c c a t t g c g

Acc65ITspMIXmaIKpnISmaI 720 35 30 25 20 PNEWDRRQLVVALSNSSPVR lacZ α M13 fwd MCS C C C A G T C A C G A C G T T G T A A A A C G M13/pUC Forward T G T A A A A C G A C G G C C A G T M13 Forward c a g g g t t t t c c c a g t c a c g a c g t t g t a a a a c g a c g g c c a g t g a a t t c g a g c t c g g t a c c c g t c c c a a a a g g g t c a g t g c t g c a a c a t t t t g c t g c c g g t c a c t t a a g c t c g a g c c a t g g g

SbfIPstIXbaIBamHI 780 15 10 5 1 PDELTSRCAHLSPTIMTM lacZ α MCS M13 rev g g g g a t c c t c t a g a g t c g a c c t g c a g g c a t g c a a g c t t g g c g t a a t c a t g g t c a t a g c t g c c c c t a g g a g a t c t c a g c t g g a c g t c c g t a c g t t c g a a c c g c a t t a g t a c c a g t a t c g a c

840 -10 M13 rev lac operator lac promoter t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t

900 -35 lac promoter CAP binding site a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t

PsiI 960 c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t a g g t c t c a g c t t c g g g t t a t a a g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a t c c a g a g t c g a a g c c c a a t a t t

NcoIStyIBsu36I 1020 t t a c c t c a g g t c g a c g t c c c a t g g t t t t g t a t a g a a t t t a c g g c t a g c g c c g g a t g c g a c a a t g g a g t c c a g c t g c a g g g t a c c a a a a c a t a t c t t a a a t g c c g a t c g c g g c c t a c g c t g

PshAI 1080 g c c g g t c g c g t c t t a t c c g g c c t t c c t a t a t c a g g c g g t g t t t a a g a c g c c g c c g c t t c g c g g c c a g c g c a g a a t a g g c c g g a a g g a t a t a g t c c g c c a c a a a t t c t g c g g c g g c g a a g c

1140 c c c a a a t c c t t a t g c c g g t t c g a c g a c t g g a c a a a a t a c t g t t t a t c t t c c c a g c g c a g g g g g t t t a g g a a t a c g g c c a a g c t g c t g a c c t g t t t t a t g a c a a a t a g a a g g g t c g c g t c c

1200 c a g g t t a a t g t a c c a c c c c a g c a g c a g c c g g t a t c c a g c g c g t a t a t a c c t t c c g g c g t a g t c c a a t t a c a t g g t g g g g t c g t c g t c g g c c a t a g g t c g c g c a t a t a t g g a a g g c c g c a t

1260 c c t t t g c c c t c c a g c g a t g c c c a g t g a c c a a a g g c g a t g c t g t a t t c t t c a g c g a c a g g g g g a a a c g g g a g g t c g c t a c g g g t c a c t g g t t t c c g c t a c g a c a t a a g a a g t c g c t g t c c c

1320 c c a g g a a t c g c a a a c c a c g g t t t c a g t g g g g c a g g g g c c t c t t c c g g c g a t t c t t a c t a g g g t c c t t a g c g t t t g g t g c c a a a g t c a c c c c g t c c c c g g a g a a g g c c g c t a a g a a t g a t c

SnaBIBsiWIPmlINsiI 1380 c t a g t a t g c a t a g g t g c t g a a a t a t a a a g t t t g t g t t t c t a a a a c a c a c g t g g t a c g t a c g a t c a t a c g t a t c c a c g a c t t t a t a t t t c a a a c a c a a a g a t t t t g t g t g c a c c a t g c a t g

BssHII 1440 g a t a a c g t a c a g t g t t t t t c c c t c c a c t t a a a t c g a a g g g t a g t g t c t t g g a g c g c g c g g c t a t t g c a t g t c a c a a a a a g g g a g g t g a a t t t a g c t t c c c a t c a c a g a a c c t c g c g c g c c

BstBI 1500 a g t a a a c a t a t a t g g t t c a t a t a t g t c c g t a g g c a c g t a a a a a a a g c g a g g g a t t c g a a t t c a t t t g t a t a t a c c a a g t a t a t a c a g g c a t c c g t g c a t t t t t t t c g c t c c c t a a g c t t a

PspOMIApaI 1560 t c c c c c g g a a c c c c c g g t t g g g g c c c a c g c c t c g a t c g a g c a a a a a a a a a a a a a a a g a a a a g g g g g c c t t g g g g g c c a a c c c c g g g t g c g g a g c t a g c t c g t t t t t t t t t t t t t t t c t t t

SacII 1620 M13 rev a a a a a a a a a a a a a a a a g c t t t c c c g c g g c c a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t t t t t t t t t t t t t t t t t c g a a a g g g c g c c g g t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a

1680 -10 M13 rev lac operator lac promoter c c t g t g t g a a a t t g t t a t c c g c t c a c a a t t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g g g a c a c a c t t t a a c a a t a g g c g a g t g t t a a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c

1740 -35 lac promoter CAP binding site t g t a a a g c c t g g g g t g c c t a a t g a g t g a g c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g a c a t t t c g g a c c c c a c g g a t t a c t c a c t c g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c

1800 c c c g c t t t c c a g t c g g g a a a c c t g t c g t g c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g c g a a a g g t c a g c c c t t t g g a c a g c a c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c

BspQISapI 1860 g g g a g a g g c g g t t t g c g t a t t g g g c g c t c t t c c g c t t c c t c a c t c a c t g a c t c g c t g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g a a g g c g a a g g a g t g a g t g a c t g a g c g a c g c g

1920 t c g g t c g c t c g g c t g c g g c g a g c g g t a t c a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a g c c a g c g a g c c g a c g c c g c t c g c c a t a g t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g

PciI 1980 a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c

2040 ori a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a

2100 ori T T T C T A T G G T C pBR322ori-F c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c

2160 ori C G C A A A G G G pBR322ori-F g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t

2220 ori t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c

2280 ori t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a

2340 ori c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g

AlwNI 2400 ori g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g

2460 ori g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a

2520 ori g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g

2580 ori g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g

2640 ori a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c

2700 a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c

2760 a t c c t t t t a a a t t a a a a a t g a a g t t t t a a a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t a g g a a a a t t t a a t t t t t a c t t c a a a a t t t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c

2820 285 280 275 *WHKILSAGIEAIQRNR AmpR *WHKILSAGIEAIQRNR t c t g a c a g t t a c c a a t g c t t a a t c a g t g a g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t a g a c t g t c a a t g g t t a c g a a t t a g t c a c t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a

AhdI 2880 270 265 260 255 EDMTAQSGTTYIVVIRSPKG AmpR EDMTAQSGTTYIVVIRSPKG t c a t c c a t a g t t g c c t g a c t c c c c g t c g t g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t

2940 250 245 240 235 DPGLAAIIGRSGREGAGSKD AmpR DPGLAAIIGRSGREGAGSKD t c t g g c c c c a g t g c t g c a a t g a t a c c g c g a g a t c c a c g c t c a c c g g c t c c a g a t t t a t c a a g a c c g g g g t c a c g a c g t t a c t a t g g c g c t c t a g g t g c g a g t g g c c g a g g t c t a a a t a g t

3000 230 225 220 215 AIFWGAPLASRLLPGAVKDA AmpR AIFWGAPLASRLLPGAVKDA g c a a t a a a c c a g c c a g c c g g a a g g g c c g a g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c c g t t a t t t g g t c g g t c g g c c t t c c c g g c t c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g

3060 210 205 200 195 EMWDILQQRSALTLLEGTLL AmpR EMWDILQQRSALTLLEGTLL t c c a t c c a g t c t a t t a a t t g t t g c c g g g a a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t a g g t a g g t c a g a t a a t t a a c a a c g g c c c t t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a

3120 190 185 180 175 KRLTTAMAVPMTTDREDNPI AmpR KRLTTAMAVPMTTDREDNPI t t g c g c a a c g t t g t t g c c a t t g c t a c a g g c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g a a c g c g t t g c a a c a a c g g t a a c g a t g t c c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c

3180 170 165 160 155 AENLEPEWRDLRTVHDGMNH AmpR AENLEPEWRDLRTVHDGMNH g c t t c a t t c a g c t c c g g t t c c c a a c g a t c a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g

3240 150 145 140 135 LFATLEKPGGITTLLLNAAT AmpR LFATLEKPGGITTLLLNAAT a a a a a a g c g g t t a g c t c c t t c g g t c c t c c g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c

3300 130 125 120 115 NDSMTIAASCLERVTMGDTL AmpR NDSMTIAASCLERVTMGDTL t t a t c a c t c a t g g t t a t g g c a g c a c t g c a t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a a a t a g t g a g t a c c a a t a c c g t c g t g a c g t a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t

ScaI 3360 110 105 100 95 HKETVPSYEVLDNQSYRIRR AmpR HKETVPSYEVLDNQSYRIRR t g c t t t t c t g t g a c t g g t g a g t a c t c a a c c a a g t c a t t c t g a g a a t a g c g t a t g c g g c g a a c g a a a a g a c a c t g a c c a c t c a t g a g t t g g t t c a g t a a g a c t c t t a t c g c a t a c g c c g c t

3420 90 85 80 75 GLQEQGADIRSLVAGCLLVK AmpR A T A A T A C C G C G C C A C A T A G C Amp-R GLQEQGADIRSLVAGCLLVK c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a g g c t c a a c g a g a a c g g g c c g c a g t t a t g c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t

XmnI 3480 70 65 60 55 FTSMMPFREEPRFSELIKGS AmpR FTSMMPFREEPRFSELIKGS a a a g t g c t c a t c a t t g g a a a a c g t t c t t c g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t t c a c g a g t a g t a a c c t t t t g c a a g a a g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c

3540 50 45 40 35 NLDLEIYGVRAGLQDEADKV AmpR NLDLEIYGVRAGLQDEADKV t t g a g a t c c a g t t c g a t g t a a c c c a c t c g t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t a a c t c t a g g t c a a g c t a c a t t g g g t g a g c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a

3600 30 25 KVLTEPH 20 15 signal sequence AFVPLCFAAFFPI AmpR KVLTEPHAFVPLCFAAFFPI t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t

SspI 3660 10 5 1 signal sequence LAVRFHQISM AmpR AmpR promoter LAVRFHQISM a g g g c g a c a c g g a a a t g t t g a a t a c t c a t a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a

3720 AmpR promoter t a t c a g g g t t a t t g t c t c a t g a g c g g a t a c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t

3780 AmpR promoter a t a g g g g t t c c g c g c a c a t t t c c c c g a a a a g t g c c a c c t g a c g t c t a a g a a a c c a t t a t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t t c a c g g t g g a c t g c a g a t t c t t t g g t a a t a a

3840 A A T A G G C G T A T C A C G A G G C pBRforEco a t c a t g a c a t t a a c c t a t a a a a a t a g g c g t a t c a c g a g g c c c t t t c g t c t c g c g c g t t t c t a g t a c t g t a a t t g g a t a t t t t t a t c c g c a t a g t g c t c c g g g a a a g c a g a g c g c g c a a a g

PfoI 3900 G G A G A C T G T G T A C G T C G A G G G C C pGEX 3' g g t g a t g a c g g t g a a a a c c t c t g a c a c a t g c a g c t c c c g g a g a c g g t c a c a g c t t g t c t g c c a c t a c t g c c a c t t t t g g a g a c t g t g t a c g t c g a g g g c c t c t g c c a g t g t c g a a c a g a c

3960 t a a g c g g a t g c c g g g a g c a g a c a a g c c c g t c a g g g c g c g t c a g c g g g t g t t g g c g g g t g t a t t c g c c t a c g g c c c t c g t c t g t t c g g g c a g t c c c g c g c a g t c g c c c a c a a c c g c c c a c a

4020 c g g g g c t g g c t t a a c t a t g c g g c a t c a g a g c a g a t t g t a c t g a g a g t g c a c c a t t c g a c g g c c c c g a c c g a a t t g a t a c g c c g t a g t c t c g t c t a a c a t g a c t c t c a c g t g g t a a g c t g c

4080 c t c t c c c t t a t g c g a c t c c t g c a t t a g g a a g c a g c c c a g t a g t a g g t t g a g g c c g t t g a g g a g a g g g a a t a c g c t g a g g a c g t a a t c c t t c g t c g g g t c a t c a t c c a a c t c c g g c a a c t c

4140 c a c c g c c g c c g c a a g g a a t g g t g c a t g c a a g g a g a t g g c g c c c a a c a g t c c c c c g g c c a c g t g g c g g c g g c g t t c c t t a c c a c g t a c g t t c c t c t a c c g c g g g t t g t c a g g g g g c c g g t g

AfeI 4200 g g g g c c t g c c a c c a t a c c c a c g c c g a a a c a a g c g c t c a t g a g c c c g a a g t g g c g a g c c c g c c c c g g a c g g t g g t a t g g g t g c g g c t t t g t t c g c g a g t a c t c g g g c t t c a c c g c t c g g g c

SgrAI 4260 G G T G A T G T C G G C G A T A T A G G pBRrevBam a t c t t c c c c a t c g g t g a t g t c g g c g a t a t a g g c g c c a g c a a c c g c a c c t g t g g c g c c g g t t a g a a g g g g t a g c c a c t a c a g c c g c t a t a t c c g c g g t c g t t g g c g t g g a c a c c g c g g c c a

NgoMIVNaeI 4281 g a t g c c g g c c a c g a t g c g t c c c t a c g g c c g g t g c t a c g c a g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.