Addgene pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR Sequencing Result - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR Sequencing Result


Map View

pBR322ori-F (13,022 .. 13,041) pENTR-R (12,775 .. 12,794) Kan-R (12,697 .. 12,716) SspI (12,432) AsiSI (12,359) AseI (12,156) BsaBI * (10,740) PvuII (10,649) EGFP-N (9674 .. 9695) FspI (6954) SgrAI (6918) SnaBI (6909) BstBI (6688) ApaLI (13,195) XcmI (307) PshAI (1046) Puro-R (1079 .. 1098) PflFI - Tth111I (1121) BstEII (1213) Puro-F (1575 .. 1595) Bsu36I (1718) Bglob-pA-R (1791 .. 1810) rbglobpA-R (1910 .. 1929) SpeI (3028) PsiI (6382) SV40 NLS pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR 13,368 bp

Sequence View

5ʹ 3ʹ 60 ori g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t

120 ori a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a

180 ori g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a

240 t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t g a g a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a c t c t

300 t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t c t c g a g c t t t c g g a g t g a g a t g g a g t g g c a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a g a g c t c g a a a g c c t c a c t c t a c c t c a c c g

XcmI 360 c a c t g t g g a a c t g g g c c t a g c a t c g c g a t a a g c t c t a g a g g g a c a g c c c c c c c c c a a a g c g t g a c a c c t t g a c c c g g a t c g t a g c g c t a t t c g a g a t c t c c c t g t c g g g g g g g g g t t t c g

420 c c c c a g g g a t g t a a t t a c g t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t g g g g t c c c t a c a t t a a t g c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a

480 c c g c t c c g g t c c g g c g c t c c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g g c g a g g c c a g g c c g c g a g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c

540 g c a c g g g g a a g g t g g c a c g g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c c g t g c c c c t t c c a c c g t g c c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g

600 t g c a g a c a c c t g g g g g g a t a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a a c g t c t g t g g a c c c c c c t a t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t

660 c a g a a t c a t a g a a c g g c c t g c g t a c g g a t g g g g c t c t a g c t c t a g a g c t t g a t a t c g a a t g t c t t a g t a t c t t g c c g g a c g c a t g c c t a c c c c g a g a t c g a g a t c t c g a a c t a t a g c t t a

720 t c c t g c a g c c c c g c t a g a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g t c a g g a c g t c g g g g c g a t c t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t g c a g

780 c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c t c c c g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g a g g g

840 c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a c g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t g c c c

900 a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g a t a c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c t a t g

960 g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c c t g g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g g a c c

1020 g c g c g c c t g g t g g c g a a c g a t c a a c a g a c g g g a g a t c t a c t a t t g g g a c a g c a g a g a t c c c g c g c g g a c c a c c g c t t g c t a g t t g t c t g c c c t c t a g a t g a t a a c c c t g t c g t c t c t a g g

PshAI 1080 1 M Bxb1 attB PuroR T A Puro-R M a g t t g g t a a t g g c t t g t c g a c g a c g g c g g t c t c c g t c g t c a g g a t c a t a g g t g c c a c c a t t c a a c c a t t a c c g a a c a g c t g c t g c c g c c a g a g g c a g c a g t c c t a g t a t c c a c g g t g g t a

Tth111IPflFI 1140 5 10 15 20 TEYKPTVRLATRDDVPRAVR PuroR C T G G C T C A T G T T C G G G T G Puro-R TEYKPTVRLATRDDVPRAVR g a c c g a g t a c a a g c c c a c g g t g c g c c t c g c c a c c c g c g a c g a c g t c c c c c g g g c c g t a c g c t g g c t c a t g t t c g g g t g c c a c g c g g a g c g g t g g g c g c t g c t g c a g g g g g c c c g g c a t g c

1200 25 30 35 40 TLAAAFADYPATRHTVDPDR PuroR TLAAAFADYPATRHTVDPDR c a c c c t c g c c g c c g c g t t c g c c g a c t a c c c c g c c a c g c g c c a c a c c g t c g a c c c g g a c c g g t g g g a g c g g c g g c g c a a g c g g c t g a t g g g g c g g t g c g c g g t g t g g c a g c t g g g c c t g g c

BstEII 1260 45 50 55 60 HIERVTELQELFLTRVGLDI PuroR HIERVTELQELFLTRVGLDI c c a c a t c g a g c g g g t c a c c g a g c t g c a a g a a c t c t t c c t c a c g c g c g t c g g g c t c g a c a t g g t g t a g c t c g c c c a g t g g c t c g a c g t t c t t g a g a a g g a g t g c g c g c a g c c c g a g c t g t a

1320 65 70 75 80 GKVWVADDGAAVAVWTTPES PuroR GKVWVADDGAAVAVWTTPES c g g c a a g g t g t g g g t c g c g g a c g a c g g c g c c g c g g t g g c g g t c t g g a c c a c g c c g g a g a g g c c g t t c c a c a c c c a g c g c c t g c t g c c g c g g c g c c a c c g c c a g a c c t g g t g c g g c c t c t c

1380 85 90 95 100 VEAGAVFAEIGPRMAELSGS PuroR VEAGAVFAEIGPRMAELSGS c g t c g a a g c g g g g g c g g t g t t c g c c g a g a t c g g c c c g c g c a t g g c c g a g t t g a g c g g t t c g c a g c t t c g c c c c c g c c a c a a g c g g c t c t a g c c g g g c g c g t a c c g g c t c a a c t c g c c a a g

1440 105 110 115 120 RLAAQQQMEGLLAPHRPKEP PuroR RLAAQQQMEGLLAPHRPKEP c c g g c t g g c c g c g c a g c a a c a g a t g g a a g g c c t c c t g g c g c c g c a c c g g c c c a a g g a g c c g g c c g a c c g g c g c g t c g t t g t c t a c c t t c c g g a g g a c c g c g g c g t g g c c g g g t t c c t c g g

1500 125 130 135 140 AWFLATVGVSPDHQGKGLGS PuroR AWFLATVGVSPDHQGKGLGS c g c g t g g t t c c t g g c c a c c g t c g g c g t c t c g c c c g a c c a c c a g g g c a a g g g t c t g g g c a g g c g c a c c a a g g a c c g g t g g c a g c c g c a g a g c g g g c t g g t g g t c c c g t t c c c a g a c c c g t c

1560 145 150 155 160 AVVLPGVEAAERAGVPAFLE PuroR AVVLPGVEAAERAGVPAFLE c g c c g t c g t g c t c c c c g g a g t g g a g g c g g c c g a g c g c g c c g g g g t g c c c g c c t t c c t g g a g c g g c a g c a c g a g g g g c c t c a c c t c c g c c g g c t c g c g c g g c c c c a c g g g c g g a a g g a c c t

1620 165 170 175 180 TSAPRNLPFYERLGFTVTAD PuroR G C A A C C T C C C C T T C T A C G A G C Puro-F TSAPRNLPFYERLGFTVTAD g a c a t c c g c g c c c c g c a a c c t c c c c t t c t a c g a g c g g c t c g g c t t c a c c g t c a c c g c c g a c t g t a g g c g c g g g g c g t t g g a g g g g a a g a t g c t c g c c g a g c c g a a g t g g c a g t g g c g g c t

1680 185 190 195 200 VEVPEGPRTWCMTRKPGA* PuroR VEVPEGPRTWCMTRKPGA* c g t c g a g g t g c c c g a a g g a c c g c g c a c c t g g t g c a t g a c c c g c a a g c c c g g t g c c t g a g c g c a g c t c c a c g g g c t t c c t g g c g c g t g g a c c a c g t a c t g g g c g t t c g g g c c a c g g a c t c g

Bsu36I 1740 t t c c a c t g g a t t g t a c a a t t a c c a a c t g a a t t c a c t c c t c a g g t g c a g g c t g c c t a t c a g a a g g t g a c c t a a c a t g t t a a t g g t t g a c t t a a g t g a g g a g t c c a c g t c c g a c g g a t a g t c

1800 A G A A A A A G G G Bglob-pA-R a a g g t g g t g g c t g g t g t g g c c a a t g c c c t g g c t c a c a a a t a c c a c t g a g a t c t t t t t c c c t t c c a c c a c c g a c c a c a c c g g t t a c g g g a c c g a g t g t t t a t g g t g a c t c t a g a a a a a g g g

1860 β- globin poly(A) signal A G A C G G T T T T Bglob-pA-R t c t g c c a a a a a t t a t g g g g a c a t c a t g a a g c c c c t t g a g c a t c t g a c t t c t g g c t a a t a a a g a c g g t t t t t a a t a c c c c t g t a g t a c t t c g g g g a a c t c g t a g a c t g a a g a c c g a t t a t t

1920 β- globin poly(A) signal G T G A G C C T T C C rbglobpA-R a g g a a a t t t a t t t t c a t t g c a a t a g t g t g t t g g a a t t t t t t g t g t c t c t c a c t c g g a a g g t c c t t t a a a t a a a a g t a a c g t t a t c a c a c a a c c t t a a a a a a c a c a g a g a g t g a g c c t t c c

1980 T G T A T A C C C rbglobpA-R a c a t a t g g g a g g g c a a a t c a t t t a a a a c a t c a g a a t g a g t a t t t g g t t t a g a g t t t g g c a t g t a t a c c c t c c c g t t t a g t a a a t t t t g t a g t c t t a c t c a t a a a c c a a a t c t c a a a c c g t

2040 a c a t a t g c c c a t a t g c t g g c t g c c a t g a a c a a a g g t t g g c t a t a a a g a g g t c a t c a g t a t t g t a t a c g g g t a t a c g a c c g a c g g t a c t t g t t t c c a a c c g a t a t t t c t c c a g t a g t c a t a

2100 a t g a a a c a g c c c c c t g c t g t c c a t t c c t t a t t c c a t a g a a a a g c c t t g a c t t g a g g t t a g t a c t t t g t c g g g g g a c g a c a g g t a a g g a a t a a g g t a t c t t t t c g g a a c t g a a c t c c a a t c

2160 a t t t t t t t t a t a t t t t g t t t t g t g t t a t t t t t t t c t t t a a c a t c c c t a a a a t t t t c c t t a t a a a a a a a a t a t a a a a c a a a a c a c a a t a a a a a a a g a a a t t g t a g g g a t t t t a a a a g g a a t

2220 c a t g t t t t a c t a g c c a g a t t t t t c c t c c t c t c c t g a c t a c t c c c a g t c a t a g c t g t c c c t g t a c a a a a t g a t c g g t c t a a a a a g g a g g a g a g g a c t g a t g a g g g t c a g t a t c g a c a g g g a

2280 c t t c t c t t a t g g a g a t c c g g g c c t a g c a t c g c g a t a a g c t c t a g a g g g a c a g c c c c c c c c g a a g a g a a t a c c t c t a g g c c c g g a t c g t a g c g c t a t t c g a g a t c t c c c t g t c g g g g g g g g

2340 c a a a g c c c c c a g g g a t g t a a t t a c g t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g t t t c g g g g g t c c c t a c a t t a a t g c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g

2400 g g g g c t c c g c t c c g g t c c g g c g c t c c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a c c c c g a g g c g a g g c c a g g c c g c g a g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t

2460 g c c c g g g c a c g g g g a a g g t g g c a c g g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t c g g g c c c g t g c c c c t t c c a c c g t g c c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a

2520 t g a g c c t g c a g a c a c c t g g g g g g a t a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a a c t c g g a c g t c t g t g g a c c c c c c t a t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t

2580 g a a t g a c a g a a t c a t a g a a c g g c c t g c g t a c g g a t g g g g c t c t a g c t c t a g a g c t t g a t a c t t a c t g t c t t a g t a t c t t g c c g g a c g c a t g c c t a c c c c g a g a t c g a g a t c t c g a a c t a t

2640 t c g a a t t c c t g c a g c c c c g c t a g a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t a g c t t a a g g a c g t c g g g g c g a t c t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a

2700 t a c g t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c a t g c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g

2760 g c t c c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c g a g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c

2820 c a c g g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g t g c c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c

2880 g g a t a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g c c t a t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c

2940 g c c t g g g c g c g c c t g g t g g c g a a c g a t c a a c a g a c g g g a g a t t g c c a a g g t c t t g a c a a a c g g a c c c g c g c g g a c c a c c g c t t g c t a g t t g t c t g c c c t c t a a c g g t t c c a g a a c t g t t t

3000 c g a g t g c t a g t a c a a g a t c g c g t c g a c g g a t c g g g a g a t c t g a a t t a g a g t g a g g a c g a t g c t c a c g a t c a t g t t c t a g c g c a g c t g c c t a g c c c t c t a g a c t t a a t c t c a c t c c t g c t a

SpeI 3060 Kozak sequence MGATSLLPELIASA c c c t c t a g a g g g t a t a t a a t g g g g g c c a c t a g t c t a c t a c c a g a g c t c a t c g c t a g c g c c g g g a g a t c t c c c a t a t a t t a c c c c c g g t g a t c a g a t g a t g g t c t c g a g t a g c g a t c g c g g

3120 1 5 10 15 MVSELIKENMHMKLYMEGT mKate2 Kozak sequence TMVSELIKENMHMKLYMEGT a c c a t g g t g a g c g a g c t g a t t a a g g a g a a c a t g c a c a t g a a g c t g t a t a t g g a g g g c a c c t g g t a c c a c t c g c t c g a c t a a t t c c t c t t g t a c g t g t a c t t c g a c a t a t a c c t c c c g t g g

3180 20 25 30 35 VNNHHFKCTSEGEGKPYEGT mKate2 VNNHHFKCTSEGEGKPYEGT g t g a a c a a c c a c c a c t t c a a g t g t a c c t c c g a g g g c g a a g g c a a g c c c t a c g a g g g c a c c c a c t t g t t g g t g g t g a a g t t c a c a t g g a g g c t c c c g c t t c c g t t c g g g a t g c t c c c g t g g

3240 40 45 50 55 QTMRIKAVEGGPLPFAFDIL mKate2 QTMRIKAVEGGPLPFAFDIL c a g a c c a t g a g a a t c a a g g c g g t c g a g g g c g g c c c t c t c c c c t t c g c c t t c g a c a t c c t g g t c t g g t a c t c t t a g t t c c g c c a g c t c c c g c c g g g a g a g g g g a a g c g g a a g c t g t a g g a c

3300 60 65 70 75 ATSFMYGSKTFINHTQGIPD mKate2 ATSFMYGSKTFINHTQGIPD g c t a c c a g c t t c a t g t a c g g c a g c a a a a c c t t c a t c a a c c a c a c c c a g g g c a t c c c c g a c c g a t g g t c g a a g t a c a t g c c g t c g t t t t g g a a g t a g t t g g t g t g g g t c c c g t a g g g g c t g

3360 80 85 90 95 FFKQSFPEGFTWERVTTYED mKate2 FFKQSFPEGFTWERVTTYED t t c t t t a a g c a g t c c t t c c c c g a g g g c t t c a c a t g g g a g a g a g t c a c c a c a t a c g a a g a t a a g a a a t t c g t c a g g a a g g g g c t c c c g a a g t g t a c c c t c t c t c a g t g g t g t a t g c t t c t a

3420 100 105 110 115 GGVLTATQDTSLQDGCLIYN mKate2 GGVLTATQDTSLQDGCLIYN g g g g g c g t g c t g a c c g c t a c c c a g g a c a c c a g c c t c c a g g a c g g c t g c c t c a t c t a c a a c c c c c c g c a c g a c t g g c g a t g g g t c c t g t g g t c g g a g g t c c t g c c g a c g g a g t a g a t g t t g

3480 120 125 130 135 VKIRGVNFPSNGPVMQKKTL mKate2 VKIRGVNFPSNGPVMQKKTL g t c a a g a t c a g a g g g g t g a a c t t c c c a t c c a a c g g c c c t g t g a t g c a g a a g a a a a c a c t c c a g t t c t a g t c t c c c c a c t t g a a g g g t a g g t t g c c g g g a c a c t a c g t c t t c t t t t g t g a g

3540 140 145 150 155 GWEASTETLYPADGGLEGRA mKate2 GWEASTETLYPADGGLEGRA g g c t g g g a g g c c t c c a c c g a a a c c c t g t a c c c c g c t g a c g g c g g c c t g g a a g g c a g a g c c c c g a c c c t c c g g a g g t g g c t t t g g g a c a t g g g g c g a c t g c c g c c g g a c c t t c c g t c t c g g

3600 160 165 170 175 DMALKLVGGGHLICNLKTTY mKate2 DMALKLVGGGHLICNLKTTY g a c a t g g c c c t g a a g c t c g t g g g c g g g g g c c a c c t g a t c t g c a a c t t g a a a a c c a c a t a c c t g t a c c g g g a c t t c g a g c a c c c g c c c c c g g t g g a c t a g a c g t t g a a c t t t t g g t g t a t g

3660 180 185 190 195 RSKKPAKNLKMPGVYYVDRR mKate2 RSKKPAKNLKMPGVYYVDRR a g a t c c a a g a a a c c c g c t a a g a a c c t c a a g a t g c c c g g c g t c t a c t a t g t g g a c a g g a g a t c t a g g t t c t t t g g g c g a t t c t t g g a g t t c t a c g g g c c g c a g a t g a t a c a c c t g t c c t c t

3720 200 205 210 215 LERIKEADKETYVEQHEVAV mKate2 LERIKEADKETYVEQHEVAV c t g g a a a g a a t c a a g g a g g c c g a c a a a g a a a c c t a c g t c g a g c a g c a c g a g g t g g c t g t g g a c c t t t c t t a g t t c c t c c g g c t g t t t c t t t g g a t g c a g c t c g t c g t g c t c c a c c g a c a c

3780 220 225 230 ARYCDLPSKLGHR* mKate2 ARYCDLPSKLGHR* g c c a g a t a c t g c g a c c t c c c t a g c a a a c t g g g g c a c a g a t g a g c g g c c g c t c g a g t c t a g c g g t c t a t g a c g c t g g a g g g a t c g t t t g a c c c c g t g t c t a c t c g c c g g c g a g c t c a g a t c

3840 bGH poly(A) signal a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a

3900 bGH poly(A) signal t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g

3960 bGH poly(A) signal c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c

4020 bGH poly(A) signal t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g t c a a t a g c a g g c a t g c t g g g g a a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c a g t t a t c g t c c g t a c g a c c c c t

4080 bGH poly(A) signal t g c g g t g g g c t c t a t g g c t t c t g a g g c a g g t c g a g g g t a c a t g c g c c t t a c t c c t t g t g a a c g c c a c c c g a g a t a c c g a a g a c t c c g t c c a g c t c c c a t g t a c g c g g a a t g a g g a a c a c t

4140 g c g c a t c g c g a t a a g c t c t a g a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g c g t a g c g c t a t t c g a g a t c t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t

4200 c g t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c g c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g

4260 t c c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a a g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t

4320 c g g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g g c c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c

4380 a t a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c t a t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g

4440 c t g c g t a c g g a t g g g g c t c t a g c t c t a g a g c t t g a t a t c g a a t t c c t g c a g c c c c g c t a g g a c g c a t g c c t a c c c c g a g a t c g a g a t c t c g a a c t a t a g c t t a a g g a c g t c g g g g c g a t c

4500 a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g t c c c t c c c c c g c t a g g g g g t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t g c a g g g a g g g g g c g a t c c c c c

4560 c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c t c c c c c c g c a t c c c c g a g c c g g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g a g g g g g g c g t a g g g g c t c g g c

4620 g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a c g g g a t c g c t t t c c t c t g a a c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t g c c c t a g c g a a a g g a g a c t t g

4680 g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g a t a c g g g g a a a a a g c t t t a g g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c t a t g c c c c t t t t t c g a a a t c c

4740 c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c c t g g g c g c g c c t g g t g g c g a a g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g g a c c c g c g c g g a c c a c c g c t t

4800 c g a t c a a c a g a c g g g a g a g g g c a a a a c c g a t g t t g a c g g a c t a a t c c t g a c a a g a t c t g g g c t a g t t g t c t g c c c t c t c c c g t t t t g g c t a c a a c t g c c t g a t t a g g a c t g t t c t a g a c c

4860 g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t

4920 c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g

4980 c a g a t a t a c g c g t g a g t a a t t c a t a c a a a a g g a c t c g c c c c t g c c t t g g g g a a t c c c a g g g t c t a t a t g c g c a c t c a t t a a g t a t g t t t t c c t g a g c g g g g a c g g a a c c c c t t a g g g t c c

5040 g a c c g t c g t t a a a c t c c c a c t a a c g t a g a a c c c a g a g a t c g c t g c g t t c c c g c c c c c t c a c t g g c a g c a a t t t g a g g g t g a t t g c a t c t t g g g t c t c t a g c g a c g c a a g g g c g g g g g a g t

5100 EF-1 α promoter c c c g c c c g c t c t c g t c a t c a c t g a g g t g g a g a a g a g c a t g c g t g a g g c t c c g g t g c c c g t g g g c g g g c g a g a g c a g t a g t g a c t c c a c c t c t t c t c g t a c g c a c t c c g a g g c c a c g g g c a

5160 EF-1 α promoter c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g g a g g g g t c g g c a a t g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c c t c c c c a g c c g t t a

5220 EF-1 α promoter t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g a t g t c g t g t a c t g g a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c t a c a g c a c a t g a c c

5280 EF-1 α promoter c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a g t c g c c g t g a a c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c a t c a g c g g c a c t t g

5340 EF-1 α promoter EF-1 α intron A g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g t g t g t g g t t c c c g c c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c a c a c a c c a a g g g c g

5400 EF-1 α promoter EF-1 α intron A *PGQTGQIVEVGR g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a c t t c c a c g c c c c t g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t g a a g g t g c g g g g a c

5460 EF-1 α promoter EF-1 α intron A AATRSEQDRAEPQFHTPSNS g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g t g g g a g a g t t c g a g c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c a c c c t c t c a a g c t c

5520 EF-1 α promoter EF-1 α intron A AKRKLLGKAEHKLQPRAQAS g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c t g g c t t g g g c g c t g c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g a c c g a a c c c g c g a c

5580 EF-1 α promoter EF-1 α intron A PGGRAFRTAGERRDRQKRYT g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t g c t t t c g a t a a g t c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a c g a a a g c t a t t c a g

5640 EF-1 α promoter EF-1 α intron A ELWKFNKIVQQSAKKRALYD t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c t g g c a a g a t a g t c t a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g a c c g t t c t a t c a g a

5700 EF-1 α promoter EF-1 α intron A QLHPGLDACQYKPKQPRPRR t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g g c c g c g g g c g g c g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c c g g c g c c c g c c g c t

5760 EF-1 α promoter EF-1 α intron A RPGHTGACMNPSAPGALAAV c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g a g c g c g g c c a c c g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c g c t c g c g c c g g t g g

5820 EF-1 α promoter EF-1 α intron A SFRVPTTELQGAQEPAQGRA g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g c c t g g c c t c g c g c c c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c g g a c c g g a g c g c g g

5880 EF-1 α promoter EF-1 α intron A ATYRGARPPLAPGTPVLQTL g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a c c a g t t g c g t g a g c c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t g g t c a a c g c a c t c g

5940 EF-1 α promoter EF-1 α intron A PFIAAERGQQLSSLISSAAS g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g a g g a c g c g g c g c t c c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c t c c t g c g c c g c g a g

6000 EF-1 α promoter EF-1 α intron A PLAPPHTVWVFSFPRETRLR g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t c c g t c c t c a g c c g t c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a g g c a g g a g t c g g c a

6060 EF-1 α promoter EF-1 α intron A RKM c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g a t t a g t t c t c g a g g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g c t a a t c a a g a g c t c

6120 EF-1 α promoter EF-1 α intron A c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g a t g g a g t t t c c c c a g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c t a c c t c a a a g g g g t

6180 EF-1 α promoter EF-1 α intron A c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a a t t c t c c t t g g a g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c a t t a a g a g g a a c c t

6240 EF-1 α promoter EF-1 α intron A a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a c a g t g g t t c a a a g t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c t g t c a c c a a g t t t c

6300 EF-1 α promoter EF-1 α intron A t t t t t t t c t t c c a t t t c a g g t g t c g t g a g a g c t c g t t t a g t g a a c c g t c a g a t c g c c t g g a a a a a a a g a a g g t a a a g t c c a c a g c a c t c t c g a g c a a a t c a c t t g g c a g t c t a g c g g a c c

6360 a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a g a c c g g g a c c g a t c c a a a t t c t c g t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t c t g g c c c t g g c t a g g t t t a a g a g c

PsiI 6420 1 5 10 DYKDHDGDYKDHDI 3xFLAG Kozak sequence MDYKDHDGDYKDHDI a g g c t a g c g c c a c c a t g g a t t a t a a g g a t c a c g a t g g c g a c t a c a a g g a c c a c g a t a t t g t c c g a t c g c g g t g g t a c c t a a t a t t c c t a g t g c t a c c g c t g a t g t t c c t g g t g c t a t a a c

6480 15 20 DYKDDDDK MA 130 PKKKRKV GIH 3xFLAG SV40 NLS DYKDDDDKMAPKKKRKVGIH a c t a c a a a g a c g a t g a c g a t a a a a t g g c t c c t a a g a a a a a g c g c a a a g t c g g t a t c c a t g t g a t g t t t c t g c t a c t g c t a t t t t a c c g a g g a t t c t t t t t c g c g t t t c a g c c a t a g g t a c

6540 (in frame with SV40 NLS) GVPVDGGLQAPPTDVSLGDE GVPVDGGLQAPPTDVSLGDE g c g t t c c c g t c g a c g g t g g a c t g c a g g c c c c a c c a a c c g a t g t t t c t t t a g g t g a t g a g t c g c a a g g g c a g c t g c c a c c t g a c g t c c g g g g t g g t t g g c t a c a a a g a a a t c c a c t a c t c a

6600 (in frame with SV40 NLS) LHLDGEDVAMAHADALDDFD LHLDGEDVAMAHADALDDFD t a c a t t t g g a c g g t g a g g a c g t a g c t a t g g c a c a t g c a g a t g c c t t a g a c g a t t t t g a c t a t g t a a a c c t g c c a c t c c t g c a t c g a t a c c g t g t a c g t c t a c g g a a t c t g c t a a a a c t g a

6660 (in frame with SV40 NLS) LDMLGDGDSPGPGFTPHDSA LDMLGDGDSPGPGFTPHDSA t g g a t a t g t t a g g a g a t g g a g a t t c a c c a g g a c c t g g t t t c a c a c c a c a c g a t t c a g c t c a c c t a t a c a a t c c t c t a c c t c t a a g t g g t c c t g g a c c a a a g t g t g g t g t g c t a a g t c g a g

BstBI 6720 (in frame with SV40 NLS) PYGALDMADFEFEQMFTDAL PYGALDMADFEFEQMFTDAL c a t a c g g t g c t t t g g a c a t g g c c g a t t t c g a a t t t g a a c a g a t g t t t a c t g a t g c c t t g g g t a t g c c a c g a a a c c t g t a c c g g c t a a a g c t t a a a c t t g t c t a c a a a t g a c t a c g g a a c c

6780 (in frame with SV40 NLS) GIDEYGGGARPGERPFQCRI GIDEYGGGARPGERPFQCRI g t a t a g a c g a a t a c g g t g g t g g a g c t a g a c c c g g g g a g c g c c c c t t c c a g t g t c g c a t t t c a t a t c t g c t t a t g c c a c c a c c t c g a t c t g g g c c c c t c g c g g g g a a g g t c a c a g c g t a a a

6840 (in frame with SV40 NLS) CMRNFSRQDRLDRHTRTHTG CMRNFSRQDRLDRHTRTHTG g c a t g c g g a a c t t t t c g c g c c a g g a c a g g c t t g a c a g g c a t a c c c g t a c t c a t a c c g g t g c g t a c g c c t t g a a a a g c g c g g t c c t g t c c g a a c t g t c c g t a t g g g c a t g a g t a t g g c c a c

6900 (in frame with SV40 NLS) EKPFQCRICMRNFSQKEHLA EKPFQCRICMRNFSQKEHLA a a a a a c c g t t t c a g t g t c g g a t c t g t a t g c g a a a t t t c t c c c a g a a g g a g c a c t t g g c g g t t t t t g g c a a a g t c a c a g c c t a g a c a t a c g c t t t a a a g a g g g t c t t c c t c g t g a a c c g c c

FspISgrAISnaBI 6960 (in frame with SV40 NLS) GHLRTHTGEKPFQCRICMRN GHLRTHTGEKPFQCRICMRN g g c a t c t a c g t a c g c a c a c c g g c g a g a a g c c a t t c c a a t g c c g a a t a t g c a t g c g c a a c t c c g t a g a t g c a t g c g t g t g g c c g c t c t t c g g t a a g g t t a c g g c t t a t a c g t a c g c g t t g a

7020 (in frame with SV40 NLS) FSRRDNLNRHLKTHLRGS* FSRRDNLNRHLKTHLRGS* t c a g t c g c c g c g a c a a c c t g a a c c g g c a c c t a a a a a c c c a c c t g a g a g g a t c t t a a g c g g a g t c a g c g g c g c t g t t g g a c t t g g c c g t g g a t t t t t g g g t g g a c t c t c c t a g a a t t c g c c

7080 bGH poly(A) signal c c g c t c g a g t c t a g a g g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t g g c g a g c t c a g a t c t c c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a

7140 bGH poly(A) signal t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a

7200 bGH poly(A) signal c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a

7260 bGH poly(A) signal t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g t c a a t a g c a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c a g t t a t c g

7320 bGH poly(A) signal a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c a g g t c g a a g a a c t a t t t c c t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g t c c a g c t t c t t g a t a a a g g

7380 t g g c t g t t a c g c g a g c g c a t c g c g a t a a g c t c t a g a g g g a c a g c c c c c c c c c a a a g c c c c a c c g a c a a t g c g c t c g c g t a g c g c t a t t c g a g a t c t c c c t g t c g g g g g g g g g t t t c g g g g

7440 c a g g g a t g t a a t t a c g t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g g t c c c t a c a t t a a t g c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c

7500 c t c c g g t c c g g c g c t c c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a g a g g c c a g g c c g c g a g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t

7560 c g g g g a a g g t g g c a c g g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c g c c c c t t c c a c c g t g c c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g

7620 a g a c a c c t g g g g g g a t a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g t c t g t g g a c c c c c c t a t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c

7680 a a t c a t a g a a c g g c c t g c g t a c g g a t g g g g c t c t a g c t c t a g a g c t t g a t a t c g a a t t c c t t a g t a t c t t g c c g g a c g c a t g c c t a c c c c g a g a t c g a g a t c t c g a a c t a t a g c t t a a g g

7740 t g c a g c c c c g c t a g a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g t c c c t a c g t c g g g g c g a t c t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t g c a g g g a

7800 c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c t c c c c c c g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g a g g g g g g

7860 g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a c g g g a t c c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t g c c c t a g

7920 g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g a t a c g g g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c t a t g c c c

7980 g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c c t g g g c g c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g g a c c c g c

8040 c g c c t g g t g g c g a a c g a t c a a c a g a c g g g a g a g g a c t a a a t a g t a g t t c a g a c g c c g t t a g c g g a c c a c c g c t t g c t a g t t g t c t g c c c t c t c c t g a t t t a t c a t c a a g t c t g c g g c a a t

8100 a g c g c a a g a t c t g g g g a g g t c g c t g a g t a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g t c g c g t t c t a g a c c c c t c c a g c g a c t c a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c

8160 c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a

8220 c g c g a t g t a c g g g c c a g a t a t a c g c g t g a g t a a t t c a t a c a a a a g g a c t c g c c c c t g c c t g c g c t a c a t g c c c g g t c t a t a t g c g c a c t c a t t a a g t a t g t t t t c c t g a g c g g g g a c g g a

8280 t g g g g a a t c c c a g g g a c c g t c g t t a a a c t c c c a c t a a c g t a g a a c c c a g a g a t c g c t g c g a c c c c t t a g g g t c c c t g g c a g c a a t t t g a g g g t g a t t g c a t c t t g g g t c t c t a g c g a c g c

8340 EF-1 α promoter t t c c c g c c c c c t c a c c c g c c c g c t c t c g t c a t c a c t g a g g t g g a g a a g a g c a t g c g t g a g a a g g g c g g g g g a g t g g g c g g g c g a g a g c a g t a g t g a c t c c a c c t c t t c t c g t a c g c a c t c

8400 EF-1 α promoter g c t c c g g t g c c c g t c a g t g g g c a g a g c g c a c a t c g c c c a c a g t c c c c g a g a a g t t g g g g g c g a g g c c a c g g g c a g t c a c c c g t c t c g c g t g t a g c g g g t g t c a g g g g c t c t t c a a c c c c c

8460 EF-1 α promoter g a g g g g t c g g c a a t t g a a c c g g t g c c t a g a g a a g g t g g c g c g g g g t a a a c t g g g a a a g t g c t c c c c a g c c g t t a a c t t g g c c a c g g a t c t c t t c c a c c g c g c c c c a t t t g a c c c t t t c a c

8520 EF-1 α promoter a t g t c g t g t a c t g g c t c c g c c t t t t t c c c g a g g g t g g g g g a g a a c c g t a t a t a a g t g c a g t a c a g c a c a t g a c c g a g g c g g a a a a a g g g c t c c c a c c c c c t c t t g g c a t a t a t t c a c g t c

8580 EF-1 α promoter EF-1 α intron A t a g t c g c c g t g a a c g t t c t t t t t c g c a a c g g g t t t g c c g c c a g a a c a c a g g t a a g t g c c g a t c a g c g g c a c t t g c a a g a a a a a g c g t t g c c c a a a c g g c g g t c t t g t g t c c a t t c a c g g c

8640 EF-1 α promoter EF-1 α intron A *PGQTGQIV t g t g t g g t t c c c g c g g g c c t g g c c t c t t t a c g g g t t a t g g c c c t t g c g t g c c t t g a a t t a a c a c a c c a a g g g c g c c c g g a c c g g a g a a a t g c c c a a t a c c g g g a a c g c a c g g a a c t t a a t

8700 EF-1 α promoter EF-1 α intron A EVGRAATRSEQDRAEPQFHT c t t c c a c g c c c c t g g c t g c a g t a c g t g a t t c t t g a t c c c g a g c t t c g g g t t g g a a g t g g g g a a g g t g c g g g g a c c g a c g t c a t g c a c t a a g a a c t a g g g c t c g a a g c c c a a c c t t c a c c c

8760 EF-1 α promoter EF-1 α intron A PSNSAKRKLLGKAEHKLQPR t g g g a g a g t t c g a g g c c t t g c g c t t a a g g a g c c c c t t c g c c t c g t g c t t g a g t t g a g g c c a c c c t c t c a a g c t c c g g a a c g c g a a t t c c t c g g g g a a g c g g a g c a c g a a c t c a a c t c c g g

8820 EF-1 α promoter EF-1 α intron A AQASPGGRAFRTAGERRDRQ t g g c t t g g g c g c t g g g g c c g c c g c g t g c g a a t c t g g t g g c a c c t t c g c g c c t g t c t c g c t a c c g a a c c c g c g a c c c c g g c g g c g c a c g c t t a g a c c a c c g t g g a a g c g c g g a c a g a g c g a

8880 EF-1 α promoter EF-1 α intron A KRYTELWKFNKIVQQSAKKR g c t t t c g a t a a g t c t c t a g c c a t t t a a a a t t t t t g a t g a c c t g c t g c g a c g c t t t t t t t c c g a a a g c t a t t c a g a g a t c g g t a a a t t t t a a a a a c t a c t g g a c g a c g c t g c g a a a a a a a g

8940 EF-1 α promoter EF-1 α intron A ALYDQLHPGLDACQYKPKQP t g g c a a g a t a g t c t t g t a a a t g c g g g c c a a g a t c t g c a c a c t g g t a t t t c g g t t t t t g g g a c c g t t c t a t c a g a a c a t t t a c g c c c g g t t c t a g a c g t g t g a c c a t a a a g c c a a a a a c c c

9000 EF-1 α promoter EF-1 α intron A RPRRRPGHTGACMNPSAPGA g c c g c g g g c g g c g a c g g g g c c c g t g c g t c c c a g c g c a c a t g t t c g g c g a g g c g g g g c c t g c g g c g c c c g c c g c t g c c c c g g g c a c g c a g g g t c g c g t g t a c a a g c c g c t c c g c c c c g g a c

9060 EF-1 α promoter EF-1 α intron A LAAVSFRVPTTELQGAQEPA c g a g c g c g g c c a c c g a g a a t c g g a c g g g g g t a g t c t c a a g c t g g c c g g c c t g c t c t g g t g g c t c g c g c c g g t g g c t c t t a g c c t g c c c c c a t c a g a g t t c g a c c g g c c g g a c g a g a c c a c

9120 EF-1 α promoter EF-1 α intron A QGRAATYRGARPPLAPGTPV c c t g g c c t c g c g c c g c c g t g t a t c g c c c c g c c c t g g g c g g c a a g g c t g g c c c g g t c g g c a g g a c c g g a g c g c g g c g g c a c a t a g c g g g g c g g g a c c c g c c g t t c c g a c c g g g c c a g c c g t

9180 EF-1 α promoter EF-1 α intron A LQTLPFIAAERGQQLSSLIS c c a g t t g c g t g a g c g g a a a g a t g g c c g c t t c c c g g c c c t g c t g c a g g g a g c t c a a a a t g g g g t c a a c g c a c t c g c c t t t c t a c c g g c g a a g g g c c g g g a c g a c g t c c c t c g a g t t t t a c c

9240 EF-1 α promoter EF-1 α intron A SAASPLAPPHTVWVFSFPRE a g g a c g c g g c g c t c g g g a g a g c g g g c g g g t g a g t c a c c c a c a c a a a g g a a a a g g g c c t t t t c c t g c g c c g c g a g c c c t c t c g c c c g c c c a c t c a g t g g g t g t g t t t c c t t t t c c c g g a a a

9300 EF-1 α promoter EF-1 α intron A TRLRRKM c c g t c c t c a g c c g t c g c t t c a t g t g a c t c c a c g g a g t a c c g g g c g c c g t c c a g g c a c c t c g g c a g g a g t c g g c a g c g a a g t a c a c t g a g g t g c c t c a t g g c c c g c g g c a g g t c c g t g g a g

9360 EF-1 α promoter EF-1 α intron A g a t t a g t t c t c g a g c t t t t g g a g t a c g t c g t c t t t a g g t t g g g g g g a g g g g t t t t a t g c g c t a a t c a a g a g c t c g a a a a c c t c a t g c a g c a g a a a t c c a a c c c c c c t c c c c a a a a t a c g c

9420 EF-1 α promoter EF-1 α intron A a t g g a g t t t c c c c a c a c t g a g t g g g t g g a g a c t g a a g t t a g g c c a g c t t g g c a c t t g a t g t a c c t c a a a g g g g t g t g a c t c a c c c a c c t c t g a c t t c a a t c c g g t c g a a c c g t g a a c t a c

9480 EF-1 α promoter EF-1 α intron A t a a t t c t c c t t g g a a t t t g c c c t t t t t g a g t t t g g a t c t t g g t t c a t t c t c a a g c c t c a g a t t a a g a g g a a c c t t a a a c g g g a a a a a c t c a a a c c t a g a a c c a a g t a a g a g t t c g g a g t c

9540 EF-1 α promoter EF-1 α intron A a c a g t g g t t c a a a g t t t t t t t c t t c c a t t t c a g g t g t c g t g a g a g c t c g t t t a g t g a a c c t g t c a c c a a g t t t c a a a a a a a g a a g g t a a a g t c c a c a g c a c t c t c g a g c a a a t c a c t t g g

9600 g t c a g a t c g c c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a g a a c g g g a c c c a g t c t a g c g g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t c t t g c c c t g g

9660 1 M 1a V 5 10 SKGEELFTG EBFP2 Kozak sequence MVSKGEELFTG g a t c c a a a t t c t c g a g g c t a g c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g c t a g g t t t a a g a g c t c c g a t c g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c

9720 15 20 25 30 VVPILVELDGDVNGHKFSVR EBFP2 G A C C A G C T C G A C C T G C C G C T G C EGFP-N VVPILVELDGDVNGHKFSVR g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g a g c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c t c

9780 35 40 45 50 GEGEGDATNGKLTLKFICTT EBFP2 GEGEGDATNGKLTLKFICTT g g g c g a g g g c g a g g g c g a t g c c a c c a a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c c c g c t c c c g c t c c c g c t a c g g t g g t t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g

9840 55 60 65 70 GKLPVPWPTLVTTLSHGVQC EBFP2 GKLPVPWPTLVTTLSHGVQC c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a g c c a c g g c g t g c a g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t c g g t g c c g c a c g t c a c

9900 75 80 85 90 FARYPDHMKQHDFFKSAMPE EBFP2 FARYPDHMKQHDFFKSAMPE c t t c g c c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a g a a g c g g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t

9960 95 100 105 110 GYVQERTIFFKDDGTYKTRA EBFP2 GYVQERTIFFKDDGTYKTRA a g g c t a c g t c c a g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a c c t a c a a g a c c c g c g c t c c g a t g c a g g t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t g g a t g t t c t g g g c g c g

10,020 115 120 125 130 EVKFEGDTLVNRIELKGVDF EBFP2 EVKFEGDTLVNRIELKGVDF *HVADLQLADVE c g a g g t g a a g t t c g a g g g c g a c a c c c t a g t g a a c c g c a t c g a g c t g a a g g g c g t c g a c t t g c t c c a c t t c a a g c t c c c g c t g t g g g a t c a c t t g g c g t a g c t c g a c t t c c c g c a g c t g a a

10,080 135 140 145 150 KEDGNILGHKLEYNFNSHNI EBFP2 KEDGNILGHKLEYNFNSHNI LLVAVDQPVLQLVVEVAVVD c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t t c a a c a g c c a c a a c a t g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a a g t t g t c g g t g t t g t a

10,140 155 160 165 170 YIMAVKQKNGIKVNFKIRHN EBFP2 YIMAVKQKNGIKVNFKIRHN IDHGDLLLVADLHVELDAVV c t a t a t c a t g g c c g t c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a g a t a t a g t a c c g g c a g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t

10,200 175 180 185 190 VEDGSVQLADHYQQNTPIGD EBFP2 VEDGSVQLADHYQQNTPIGD HLVAAHLEGVVVLLVGGDAV c g t g g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a g c a c c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t

10,260 195 200 205 210 GPVLLPDSHYLSTQSVLSKD EBFP2 GPVLLPDSHYLSTQSVLSKD AGHQQGVAVVQAGLGHQAFV c g g c c c c g t g c t g c t g c c c g a c a g c c a c t a c c t g a g c a c c c a g t c c g t g c t g a g c a a a g a g c c g g g g c a c g a c g a c g g g c t g t c g g t g a t g g a c t c g t g g g t c a g g c a c g a c t c g t t t c t

10,320 215 220 225 230 PNEKRDHMVLLEFRTAAGIT EBFP2 PNEKRDHMVLLEFRTAAGIT GVLLAIVHDQQLEAGGGPDS c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g t t c c g c a c c g c c g c c g g g a t c a c g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t c a a g g c g t g g c g g c g g c c c t a g t g

10,380 235 LGMDELYK (in frame with EBFP2) EFGSKLGSG 1 ATN EBFP2 P2A LGMDELYKEFGSKLGSGATN EAHVLQVLFETALKTTSCSV t c t c g g c a t g g a c g a g c t g t a c a a g g a a t t c g g t a g c a a g c t t g g t a g t g g a g c a a c t a a a g a g c c g t a c c t g c t c g a c a t g t t c c t t a a g c c a t c g t t c g a a c c a t c a c c t c g t t g a t t

10,440 5 10 15 FSLLKQAGDVEENPGP APLK P2A FSLLKQAGDVEENPGPAPLK KGQEFLGPIYLLVGAGGGKL c t t t t c c c t g c t c a a a c a a g c c g g g g a t g t a g a g g a g a a c c c c g g c c c c g c c c c c c t t a a g a a a a g g g a c g a g t t t g t t c g g c c c c t a c a t c t c c t c t t g g g g c c g g g g c g g g g g g a a t t

10,500 1 5 10 15 20 MAKPLSQEESTLIERATATI BSD MAKPLSQEESTLIERATATI HGLRQRLFFGGENFSCRSCD g a t g g c c a a g c c t t t g t c t c a a g a a g a a t c c a c c c t c a t t g a a a g a g c a a c g g c t a c a a t c t a c c g g t t c g g a a a c a g a g t t c t t c t t a g g t g g g a g t a a c t t t c t c g t t g c c g a t g t t a

10,560 25 30 35 40 NSIPISEDYSVASAALSSDG BSD NSIPISEDYSVASAALSSDG VADGDRLVVADGACSERAVA c a a c a g c a t c c c c a t c t c t g a g g a c t a c a g c g t c g c c a g c g c a g c t c t c t c t a g c g a c g g g t t g t c g t a g g g g t a g a g a c t c c t g a t g t c g c a g c g g t c g c g t c g a g a g a g a t c g c t g c c

10,620 45 50 55 60 RIFTGVNVYHFTGGPCAELV BSD RIFTGVNVYHFTGGPCAELV ADESTDIYIMKSPSRTCFEH c c g c a t c t t c a c t g g t g t c a a t g t a t a t c a t t t t a c t g g g g g a c c t t g t g c a g a a c t c g t g g c g t a g a a g t g a c c a c a g t t a c a t a t a g t a a a a t g a c c c c c t g g a a c a c g t c t t g a g c a

PvuII 10,680 65 70 75 80 VLGTAAAAAAGNLTCIVAIG BSD VLGTAAAAAAGNLTCIVAIG HQASSSSRCSAVQSTDDRDS g g t g c t g g g c a c t g c t g c t g c t g c g g c a g c t g g c a a c c t g a c t t g t a t c g t c g c g a t c g g c c a c g a c c c g t g a c g a c g a c g a c g c c g t c g a c c g t t g g a c t g a a c a t a g c a g c g c t a g c c

BsaBI* 10,740 85 90 95 100 NENRGILSPCGRCRQVLLDL BSD NENRGILSPCGRCRQVLLDL ILVPADQAGASPASLHKEIQ a a a t g a g a a c a g g g g c a t c t t g a g c c c c t g c g g a c g g t g c c g a c a g g t g c t t c t c g a t c t t t t a c t c t t g t c c c c g t a g a a c t c g g g g a c g c c t g c c a c g g c t g t c c a c g a a g a g c t a g a

10,800 105 110 115 120 HPGIKAIVKDSDGQPTAVGI BSD HPGIKAIVKDSDGQPTAVGI M g c a t c c t g g g a t c a a a g c c a t a g t g a a g g a c a g t g a t g g a c a g c c g a c g g c a g t t g g g a t c g t a g g a c c c t a g t t t c g g t a t c a c t t c c t g t c a c t a c c t g t c g g c t g c c g t c a a c c c t a

10,860 125 130 RELLPSGYVWEG* BSD RELLPSGYVWEG* t c g t g a a t t g c t g c c c t c t g g t t a t g t g t g g g a g g g c t a a g c g g c c g c t c g a g t c t a g a g a g c a c t t a a c g a c g g g a g a c c a a t a c a c a c c c t c c c g a t t c g c c g g c g a g c t c a g a t c t c

10,920 bGH poly(A) signal g g c c c g t t t a a a c c c g c t g a t c a g c c t c g a c t g t g c c t t c t a g t t g c c a g c c a t c t g t t g c c g g g c a a a t t t g g g c g a c t a g t c g g a g c t g a c a c g g a a g a t c a a c g g t c g g t a g a c a a c

10,980 bGH poly(A) signal t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t g c c a c t c c c a c t g t c c t t t c c t a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a c g g t g a g g g t g a c a g g a a a g g a

11,040 bGH poly(A) signal a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g t g t c a t t c t a t t c t g g g g g g t g t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c a c a g t a a g a t a a g a c c c c c c a c

11,100 bGH poly(A) signal g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g t c a a t a g c a g g c a t g c t g g g g a t g c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c a g t t a t c g t c c g t a c g a c c c c t a c

11,160 bGH poly(A) signal c g g t g g g c t c t a t g g c t t c t g a g g c a g g t c g a a g a g a c t g c c c g a c a c a t c t t a g t g a g c g c c a c c c g a g a t a c c g a a g a c t c c g t c c a g c t t c t c t g a c g g g c t g t g t a g a a t c a c t c g

11,220 g c a t c g c g a t a a g c t c t a g a g g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g c g t a g c g c t a t t c g a g a t c t c c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t g c

11,280 t c c c t c c c c c g c t a g g g g g c a g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c t c a g g g a g g g g g c g a t c c c c c g t c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g a g

11,340 c c c c c g c a t c c c c g a g c c g g c a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a c g g g g g g c g t a g g g g c t c g g c c g t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t g c

11,400 g g a t c g c t t t c c t c t g a a c g c t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g a t c c t a g c g a a a g g a g a c t t g c g a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c t a

11,460 a c g g g g a a a a a g c t t t a g g c t g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c c t t g c c c c t t t t t c g a a a t c c g a c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g g a

11,520 g c g t a c g g a t g g g g c t c t a g c t c t a g a g c t t g a t a t c g a a t t c c t g c a g c c c c g c t a g a g c g c a t g c c t a c c c c g a g a t c g a g a t c t c g a a c t a t a g c t t a a g g a c g t c g g g g c g a t c t c

11,580 g g a c a g c c c c c c c c c a a a g c c c c c a g g g a t g t a a t t a c g t c c c t c c c c c g c t a g g g g g c a c c t g t c g g g g g g g g g t t t c g g g g g t c c c t a c a t t a a t g c a g g g a g g g g g c g a t c c c c c g t

11,640 g c a g c g a g c c g c c c g g g g c t c c g c t c c g g t c c g g c g c t c c c c c c g c a t c c c c g a g c c g g c c g t c g c t c g g c g g g c c c c g a g g c g a g g c c a g g c c g c g a g g g g g g c g t a g g g g c t c g g c c g

11,700 a g c g t g c g g g g a c a g c c c g g g c a c g g g g a a g g t g g c a c g g g a t c g c t t t c c t c t g a a c g c t c g c a c g c c c c t g t c g g g c c c g t g c c c c t t c c a c c g t g c c c t a g c g a a a g g a g a c t t g c g

11,760 t t c t c g c t g c t c t t t g a g c c t g c a g a c a c c t g g g g g g a t a c g g g g a a a a a g c t t t a g g c t a a g a g c g a c g a g a a a c t c g g a c g t c t g t g g a c c c c c c t a t g c c c c t t t t t c g a a a t c c g a

11,820 g a a a g a g a g a t t t a g a a t g a c a g a a t c a t a g a a c g g c c t g g g c g c g c c t g g t g g c g a a c g c t t t c t c t c t a a a t c t t a c t g t c t t a g t a t c t t g c c g g a c c c g c g c g g a c c a c c g c t t g c

11,880 a t c a a c a g a c g g g a g a g g t t a c g a g g a t g c a c a t g t g a c c g a g g g a t g g a a c g a c g c g t a t a g t t g t c t g c c c t c t c c a a t g c t c c t a c g t g t a c a c t g g c t c c c t a c c t t g c t g c g c a t

11,940 a c t c a c g t t a a g g g a t t t t g g t c a t g a g c t t g c g c c g t c c c g t c a a g t c a g c g t a a t g c t t g a g t g c a a t t c c c t a a a a c c a g t a c t c g a a c g c g g c a g g g c a g t t c a g t c g c a t t a c g a

12,000 270 265 *FFEDLMLH KanR *FFEDLMLH c t g c c a g t g t t a c a a c c a a t t a a c c a a t t c t g a t t a g a a a a a c t c a t c g a g c a t c a a a t g g a c g g t c a c a a t g t t g g t t a a t t g g t t a a g a c t a a t c t t t t t g a g t a g c t c g t a g t t t a c

12,060 260 255 250 245 FQLKNMDPNDIGYKQFLRKQ KanR FQLKNMDPNDIGYKQFLRKQ a a a c t g c a a t t t a t t c a t a t c a g g a t t a t c a a t a c c a t a t t t t t g a a a a a g c c g t t t c t g t t t g a c g t t a a a t a a g t a t a g t c c t a a t a g t t a t g g t a t a a a a a c t t t t t c g g c a a a g a c

12,120 240 235 230 225 LSPSFEGLCNWLIALDQYRD KanR LSPSFEGLCNWLIALDQYRD t a a t g a a g g a g a a a a c t c a c c g a g g c a g t t c c a t a g g a t g g c a a g a t c c t g g t a t c g g t c a t t a c t t c c t c t t t t g a g t g g c t c c g t c a a g g t a t c c t a c c g t t c t a g g a c c a t a g c c a g

AseI 12,180 220 215 210 205 AIGVRGVDICGILKGEDFIL KanR AIGVRGVDICGILKGEDFIL t g c g a t t c c g a c t c g t c c a a c a t c a a t a c a a c c t a t t a a t t t c c c c t c g t c a a a a a t a a g a c g c t a a g g c t g a g c a g g t t g t a g t t a t g t t g g a t a a t t a a a g g g g a g c a g t t t t t a t t c

12,240 200 195 190 185 NDLSFDGHTVVSDPSFPLLK KanR NDLSFDGHTVVSDPSFPLLK g t t a t c a a g t g a g a a a t c a c c a t g a g t g a c g a c t g a a t c c g g t g a g a a t g g c a a a a g t t t c a a t a g t t c a c t c t t t a g t g g t a c t c a c t g c t g a c t t a g g c c a c t c t t a c c g t t t t c a a a

12,300 180 175 170 165 HMEKWVQEVPWGNREDDFDS KanR HMEKWVQEVPWGNREDDFDS a t g c a t t t c t t t c c a g a c t t g t t c a a c a g g c c a g c c a t t a c g c t c g t c a t c a a a a t c a c t t a c g t a a a g a a a g g t c t g a a c a a g t t g t c c g g t c g g t a a t g c g a g c a g t a g t t t t a g t g a

AsiSI 12,360 160 155 150 145 ADVLGNNMRSQAQALRFVRD KanR ADVLGNNMRSQAQALRFVRD c g c a t c a a c c a a a c c g t t a t t c a t t c g t g a t t g c g c c t g a g c g a g a c g a a a t a c g c g a t c g c g t a g t t g g t t t g g c a a t a a g t a a g c a c t a a c g c g g a c t c g c t c t g c t t t a t g c g c t a g

12,420 140 135 130 125 SNFPCNCVPISHLRRLFVAL KanR SNFPCNCVPISHLRRLFVAL g c t g t t a a a a g g a c a a t t a c a a a c a g g a a t c g a a t g c a a c c g g c g c a g g a a c a c t g c c a g c g a c a a t t t t c c t g t t a a t g t t t g t c c t t a g c t t a c g t t g g c c g c g t c c t t g t g a c g g t c

SspI 12,480 120 115 110 105 ADVINEGSDPYEELVQFATK KanR ADVINEGSDPYEELVQFATK c g c a t c a a c a a t a t t t t c a c c t g a a t c a g g a t a t t c t t c t a a t a c c t g g a a t g c t g t t t t g c g t a g t t g t t a t a a a a g t g g a c t t a g t c c t a t a a g a a g a t t a t g g a c c t t a c g a c a a a a

12,540 100 95 90 85 GPIATTLLWADDPTRIFHKI KanR GPIATTLLWADDPTRIFHKI t c c g g g g a t c g c a g t g g t g a g t a a c c a t g c a t c a t c a g g a g t a c g g a t a a a a t g c t t g a t a g g c c c c t a g c g t c a c c a c t c a t t g g t a c g t a g t a g t c c t c a t g c c t a t t t t a c g a a c t a

12,600 80 75 70 65 TPLPMFETLWNLRVMEDTVD KanR TPLPMFETLWNLRVMEDTVD g g t c g g a a g a g g c a t a a a t t c c g t c a g c c a g t t t a g t c t g a c c a t c t c a t c t g t a a c a t c c c a g c c t t c t c c g t a t t t a a g g c a g t c g g t c a a a t c a g a c t g g t a g a g t a g a c a t t g t a g

12,660 60 55 50 45 NAVSGKGHKLFLEPADPKGY KanR NAVSGKGHKLFLEPADPKGY a t t g g c a a c g c t a c c t t t g c c a t g t t t c a g a a a c a a c t c t g g c g c a t c g g g c t t c c c a t a t a a c c g t t g c g a t g g a a a c g g t a c a a a g t c t t t g t t g a g a c c g c g t a g c c c g a a g g g t a t

12,720 40 35 30 25 LRYITAGSQGVNDRAWKYGY KanR A T C G C G A G C C C A T T T A T A C C Kan-R LRYITAGSQGVNDRAWKYGY c a a g c g a t a g a t t g t c g c a c c t g a t t g c c c g a c a t t a t c g c g a g c c c a t t t a t a c c c a t a g t t c g c t a t c t a a c a g c g t g g a c t a a c g g g c t g t a a t a g c g c t c g g g t a a a t a t g g g t a t

12,780 20 15 10 5 LDADMNSNLRPRSTERQIHS KanR A T G G C T pENTR-R LDADMNSNLRPRSTERQIHS t a a a t c a g c a t c c a t g t t g g a a t t t a a t c g c g g c c t c g a c g t t t c c c g t t g a a t a t g g c t a t t t a g t c g t a g g t a c a a c c t t a a a t t a g c g c c g g a g c t g c a a a g g g c a a c t t a t a c c g a

12,840 1 M KanR C A T A A C A C C C C T T G pENTR-R M c a t a a c a c c c c t t g t a t t a c t g t t t a t g t a a g c a g a c a g t t t t a t t g t t c a t g a t g a t a t g t a t t g t g g g g a a c a t a a t g a c a a a t a c a t t c g t c t g t c a a a a t a a c a a g t a c t a c t a t a

12,900 a t t t t t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a c g g g c c a t g c t a g c t c c a g t a a a a a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t g t g c c c g g t a c g a t c g a g g t c

12,960 ori c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g c t c c g c c c c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c g a g g c g g g g

13,020 ori c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g a c a g g a c t a g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c t g t c c t g a t

13,080 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t c c g a c c c t g a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a g g c t g g g a c

13,140 ori c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t t c t c a t a g c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a a g a g t a t c g

ApaLI 13,200 ori t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c t g t g t g c a c a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g a c a c a c g t g

13,260 ori g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t g a g t c c a a c c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a c t c a g g t t g

13,320 ori c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t a g c a g a g c g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a t c g t c t c g c

3ʹ 5ʹ 13,368 ori a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.