Addgene pYX233-b2-DHFR - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: pYX233-b2-DHFR


Map View

1000 750 500 250 End (1071) PfoI * (1016) EcoNI (1009) BsaHI (1001) AflIII (944) ApoI (931) MmeI (834) BsaWI (816) AflII (798) BstXI (786) BseRI (776) SacI - BanII - BsiHKAI - Bsp1286I (768) Eco53kI (766) BssSI - BssSαI (758) AseI (722) DraI (710) CsiI - SexAI * (676) AlwNI (654) EarI (636) ScaI (607) TatI (605) XmnI (598) Bpu10I (589) BsrBI (588) BsaI (563) PflMI (549) MflI * BstYI BamHI (496) KflI PpuMI EcoO109I (484) AvaI - BsoBI (469) ClaI - BspDI (452) NspI (439) BtsI - BtsαI (413) BstZ17I (339) BsaAI - SnaBI (336) BclI * (323) TsoI (320) MspA1I (280) MscI (227) EaeI (225) BtgI (200) SpeI (193) BstBI (125) BsiWI (92) Start (0) DHFR pYX233-b2-DHFR 1071 bp

Sequence View

Start (0) 60 MLKYKPLLKISKNCEAAILR a t g c t a a a a t a c a a a c c t t t a c t a a a a a t c t c g a a g a a c t g t g a g g c t g c t a t c c t c a g a t a c g a t t t t a t g t t t g g a a a t g a t t t t t a g a g c t t c t t g a c a c t c c g a c g a t a g g a g t c t

BsiWI 120 ASKTRLNTIRAYGSTVPKSK g c g t c t a a g a c t a g a t t g a a c a c a a t c c g c g c g t a c g g t t c t a c c g t t c c a a a a t c c a a g c g c a g a t t c t g a t c t a a c t t g t g t t a g g c g c g c a t g c c a a g a t g g c a a g g t t t t a g g t t c

BstBI 180 SFEQDSRKRTQSWTALRVGA t c g t t c g a a c a a g a c t c a a g a a a a c g c a c a c a g t c a t g g a c t g c c t t g a g a g t c g g t g c a a g c a a g c t t g t t c t g a g t t c t t t t g c g t g t g t c a g t a c c t g a c g g a a c t c t c a g c c a c g t

EaeIMscIBtgISpeI 240 ILAATSSVAYLNWHNGQIDN a t t c t a g c c g c t a c t a g t t c c g t g g c g t a t c t a a a c t g g c a t a a t g g c c a a a t a g a c a a c t a a g a t c g g c g a t g a t c a a g g c a c c g c a t a g a t t t g a c c g t a t t a c c g g t t t a t c t g t t g

MspA1I 300 EPKLDMNKQKISPAEVAKHN g a g c c g a a a c t g g a t a t g a a t a a a c a a a a g a t t t c g c c c g c t g a a g t t g c c a a g c a t a a c c t c g g c t t t g a c c t a t a c t t a t t t g t t t t c t a a a g c g g g c g a c t t c a a c g g t t c g t a t t g

SnaBIBsaAIBstZ17ITsoIBclI* 360 KPDDCWVVINGYVYDLTRFL a a g c c c g a t g a t t g t t g g g t t g t g a t c a a t g g t t a c g t a t a c g a c t t a a c g c g a t t c c t a t t c g g g c t a c t a a c a a c c c a a c a c t a g t t a c c a a t g c a t a t g c t g a a t t g c g c t a a g g a t

BtsαIBtsI 420 PNHPGGQDVIKFNAGKDVTA c c a a a t c a t c c a g g t g g g c a g g a t g t t a t c a a g t t t a a t g c c g g g a a a g a t g t c a c t g c t g g t t t a g t a g g t c c a c c c g t c c t a c a a t a g t t c a a a t t a c g g c c c t t t c t a c a g t g a c g a

BsoBIAvaIBspDIClaINspI 480 IFEPLHAPNVIDKYIAPEKK a t t t t t g a a c c a t t a c a t g c t c c t a a t g t c a t c g a t a a g t a t a t a g c t c c c g a g a a a a a a t a a a a a c t t g g t a a t g t a c g a g g a t t a c a g t a g c t a t t c a t a t a t c g a g g g c t c t t t t t t

BamHIBstYIMflI*EcoO109IPpuMIKflI 540 1 5 10 MVRPLNCIVAV DHFR LGPLQGSGIMVRPLNCIVAV t t g g g t c c c c t t c a a g g a t c c g g c a t c a t g g t t c g a c c a t t g a a c t g c a t c g t c g c c g t g a a c c c a g g g g a a g t t c c t a g g c c g t a g t a c c a a g c t g g t a a c t t g a c g t a g c a g c g g c a c

XmnIBsrBIBpu10IBsaIPflMI 600 15 20 25 30 SQNMGIGKNGDLPWPPLRNE DHFR SQNMGIGKNGDLPWPPLRNE t c c c a a a a t a t g g g g a t t g g c a a g a a c g g a g a c c t a c c c t g g c c t c c g c t c a g g a a c g a g a g g g t t t t a t a c c c c t a a c c g t t c t t g c c t c t g g a t g g g a c c g g a g g c g a g t c c t t g c t c

AlwNIEarITatIScaI 660 35 40 45 50 FKYFQRMTTTSSVEGKQNLV DHFR FKYFQRMTTTSSVEGKQNLV t t c a a g t a c t t c c a a a g a a t g a c c a c a a c c t c t t c a g t g g a a g g t a a a c a g a a t c t g g t g a a g t t c a t g a a g g t t t c t t a c t g g t g t t g g a g a a g t c a c c t t c c a t t t g t c t t a g a c c a c

DraISexAI*CsiI 720 55 60 65 70 IMGRKTWFSIPEKNRPLKDR DHFR IMGRKTWFSIPEKNRPLKDR a t t a t g g g t a g g a a a a c c t g g t t c t c c a t t c c t g a g a a g a a t c g a c c t t t a a a g g a c a g a t a a t a c c c a t c c t t t t g g a c c a a g a g g t a a g g a c t c t t c t t a g c t g g a a a t t t c c t g t c t

BseRIBssSαIBssSIEco53kIBsp1286IBsiHKAIBanIISacIAseI 780 75 80 85 90 INIVLSRELKEPPRGAHFLA DHFR INIVLSRELKEPPRGAHFLA a t t a a t a t a g t t c t c a g t a g a g a a c t c a a a g a a c c a c c a c g a g g a g c t c a t t t t c t t g c c t a a t t a t a t c a a g a g t c a t c t c t t g a g t t t c t t g g t g g t g c t c c t c g a g t a a a a g a a c g g

MmeIBsaWIAflIIBstXI 840 95 100 105 110 KSLDDALRLIEQPELASKVD DHFR KSLDDALRLIEQPELASKVD a a a a g t t t g g a t g a t g c c t t a a g a c t t a t t g a a c a a c c g g a a t t g g c a a g t a a a g t a g a c t t t t c a a a c c t a c t a c g g a a t t c t g a a t a a c t t g t t g g c c t t a a c c g t t c a t t t c a t c t g

900 115 120 125 130 MVWIVGGSSVYQEAMNQPGH DHFR MVWIVGGSSVYQEAMNQPGH a t g g t t t g g a t a g t c g g a g g c a g t t c t g t t t a c c a g g a a g c c a t g a a t c a a c c a g g c c a c t a c c a a a c c t a t c a g c c t c c g t c a a g a c a a a t g g t c c t t c g g t a c t t a g t t g g t c c g g t g

AflIIIApoI 960 135 140 145 150 LRLFVTRIMQEFESDTFFPE DHFR LRLFVTRIMQEFESDTFFPE c t c a g a c t c t t t g t g a c a a g g a t c a t g c a g g a a t t t g a a a g t g a c a c g t t t t t c c c a g a a g a g t c t g a g a a a c a c t g t t c c t a g t a c g t c c t t a a a c t t t c a c t g t g c a a a a a g g g t c t t

PfoI*EcoNIBsaHI 1020 155 160 165 170 IDLGKYKLLPEYPGVLSEVQ DHFR IDLGKYKLLPEYPGVLSEVQ a t t g a t t t g g g g a a a t a t a a a c t t c t c c c a g a a t a c c c a g g c g t c c t c t c t g a g g t c c a g t a a c t a a a c c c c t t t a t a t t t g a a g a g g g t c t t a t g g g t c c g c a g g a g a g a c t c c a g g t c

End (1071) 1071 175 180 185 EEKGIKYKFEVYEKKD* DHFR EEKGIKYKFEVYEKKD* g a g g a a a a a g g c a t c a a g t a t a a g t t t g a a g t c t a c g a g a a g a a a g a c t a a c t c c t t t t t c c g t a g t t c a t a t t c a a a c t t c a g a t g c t c t t c t t t c t g a t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.