Addgene pSWD95D293A Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pSWD95D293A Sequencing Result


Map View

ScaI (8135) SspI (8030) ApoI - EcoRI (7718) CAT-R (7571 .. 7590) ApaLI (6844) pBR322ori-F (6671 .. 6690) M13/pUC Forward (6386 .. 6408) M13 Forward (6377 .. 6394) M13 fwd SmaI (6345) TspMI - XmaI (6343) BsrGI (6324) BsaAI - SnaBI (6262) SacI (5976) Eco53kI (5974) BspQI - SapI (5631) NruI (5217) BglII (4995) FspAI (4868) EGFP-C (4006 .. 4027) NotI (8258) SK primer HindIII (453) CsiI - SexAI * (909) PflFI - Tth111I (2734) PspXI (2753) NdeI (2775) RsrII (2909) FseI (2928) BbvCI (3021) SphI (3208) AhdI (3319) Acc65I (3665) KpnI (3669) pSWD95D293A 8265 bp

Sequence View

60 oriT c c g g c c a g c c t c g c a g a g c a g g a t t c c c g t t g a g c a c c g c c a g g t g c g a a t a a g g g a c a g g g c c g g t c g g a g c g t c t c g t c c t a a g g g c a a c t c g t g g c g g t c c a c g c t t a t t c c c t g t c

120 oriT t g a a g a a g g a a c a c c c g c t c g c g g g t g g g c c t a c t t c a c c t a t c c t g c c c g g c t g a c g c c a c t t c t t c c t t g t g g g c g a g c g c c c a c c c g g a t g a a g t g g a t a g g a c g g g c c g a c t g c g g

180 g t t g g a t a c a c c a a g g a a a g t c t a c a c g a a c c c t t t g g c a a a a t c c t g t a t a t c g t g c g a c a a c c t a t g t g g t t c c t t t c a g a t g t g c t t g g g a a a c c g t t t t a g g a c a t a t a g c a c g c t

240 a a a a g g a t g g a t a t a c c g a a a a a a t c g c t a t a a t g a c c c c g a a g c a g g g t t a t g c a g c g g t t t t c c t a c c t a t a t g g c t t t t t t a g c g a t a t t a c t g g g g c t t c g t c c c a a t a c g t c g c c

300 SK primer rrnB T1 terminator a a a a g a t c c g t c g a c g g c c g c t c t a g a a c t a g t g g a t c c a a a t a a a a c g a a a g g c t c a g t t t t t c t a g g c a g c t g c c g g c g a g a t c t t g a t c a c c t a g g t t t a t t t t g c t t t c c g a g t c a

360 rrnB T1 terminator c g a a a g a c t g g g c c t t t c g t t t t a t c t g t t g t t t g t c g c a t t a t a c g c a a g g c g a c a a g g g c t t t c t g a c c c g g a a a g c a a a a t a g a c a a c a a a c a g c g t a a t a t g c g t t c c g c t g t t c c

420 t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t c c a t g t c g g c a a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a g g t a c a g c c g t

HindIII 480 g a a t g c t t a a t g a a t t a c a a c a g t t t t t a t a t a a g c t t a g c g c t a c c g g a c t c a g a t c c a c t t a c g a a t t a c t t a a t g t t g t c a a a a a t a t a t t c g a a t c g c g a t g g c c t g a g t c t a g g t

540 MYRLNWWKHVEPRQGLMK g a t c g a a a t g t a c c g g c t g a a c t g g t g g a a g c a c g t t g a g c c t c g t c a a g g a c t c a t g a a c t a g c t t t a c a t g g c c g a c t t g a c c a c c t t c g t g c a a c t c g g a g c a g t t c c t g a g t a c t t

600 RVPALSRLLVTPEVSKHRLF g c g c g t c c c t g c t c t c a g t c g c c t g c t c g t t a c g c c t g a g g t g t c a a a a c a c c g t c t g t t c g c g c a g g g a c g a g a g t c a g c g g a c g a g c a a t g c g g a c t c c a c a g t t t t g t g g c a g a c a a

660 IWLDARVLPDHKLQVVTLDD c a t t t g g c t a g a c g c t a g a g t t c t t c c g g a t c a c a a g c t c c a g g t c g t t a c a c t c g a t g a g t a a a c c g a t c t g c g a t c t c a a g a a g g c c t a g t g t t c g a g g t c c a g c a a t g t g a g c t a c t

720 DCSFGVLHSRFHEVWALAAG c g a c t g c t c a t t c g g a g t t t t a c a c t c a a g g t t c c a c g a g g t g t g g g c t c t t g c a g c g g g g c t g a c g a g t a a g c c t c a a a a t g t g a g t t c c a a g g t g c t c c a c a c c c g a g a a c g t c g c c c

780 SWHGSGNDPRYTISTTFETF g t c g t g g c a c g g c t c a g g c a a t g a t c c g c g c t a c a c c a t c t c c a c c a c c t t c g a g a c c t t c a g c a c c g t g c c g a g t c c g t t a c t a g g c g c g a t g t g g t a g a g g t g g t g g a a g c t c t g g a a

840 PFPEGLTPNIAAVDYEGDPR c c c c t t c c c g g a a g g c c t g a c g c c c a a c a t a g c c g c c g t c g a c t a t g a g g g c g a c c c g c g g g g g a a g g g c c t t c c g g a c t g c g g g t t g t a t c g g c g g c a g c t g a t a c t c c c g c t g g g c g c

900 AQAIAAAAAELNRLREAWLN c g c c c a g g c c a t c g c c g c g g c g g c g g c c g a g c t g a a c c g g c t g c g g g a a g c c t g g c t c a a g c g g g t c c g g t a g c g g c g c c g c c g c c g g c t c g a c t t g g c c g a c g c c c t t c g g a c c g a g t t

SexAI*CsiI 960 PPDLVRIEPEVVPGYPDRVL t c c g c c c g a c c t g g t g c g g a t c g a g c c c g a g g t c g t g c c c g g c t a t c c c g a c c g c g t g c t a g g c g g g c t g g a c c a c g c c t a g c t c g g g c t c c a g c a c g g g c c g a t a g g g c t g g c g c a c g a

1020 PVSPEAGAELKKRTLTNLYN g c c c g t c a g c c c c g a g g c g g g c g c c g a g c t g a a g a a g c g c a c c c t g a c c a a c c t c t a c a a c g g g c a g t c g g g g c t c c g c c c g c g g c t c g a c t t c t t c g c g t g g g a c t g g t t g g a g a t g t t

1080 QRPAWLDMAHQRLDAAVAAA c c a g c g c c c g g c c t g g c t g g a c a t g g c c c a c c a g c g c c t g g a c g c c g c c g t c g c c g c c g c g g t c g c g g g c c g g a c c g a c c t g t a c c g g g t g g t c g c g g a c c t g c g g c g g c a g c g g c g g c g

1140 YGWPDGLTDDEILERLFALN c t a c g g c t g g c c c g a c g g c c t g a c c g a c g a c g a g a t c c t g g a g c g c c t g t t c g c c c t c a a g a t g c c g a c c g g g c t g c c g g a c t g g c t g c t g c t c t a g g a c c t c g c g g a c a a g c g g g a g t t

1200 QERAAAGR* c c a g g a g c g c g c g g c g g c g g g g c g g t a g g t t a g a c c c c t c c a a a a c c c c g a c c t c c c c g g g g t c c t c g c g c g c c g c c g c c c c g c c a t c c a a t c t g g g g a g g t t t t g g g g c t g g a g g g g c c

1260 c g a a g g c c g g g g t c c a g t t a c a t c c g g a a c g c t t g g a g t g g g c c c g c c t a c g g c c t c g a c g c t t c c g g c c c c a g g t c a a t g t a g g c c t t g c g a a c c t c a c c c g g g c g g a t g c c g g a g c t g

1320 c a a c c c g c a g t c g g c a a g g g g t t c g a t c t g g a c c c c g g c a t t c g c c g g g g a g g t c g g c a g g t t g g g c g t c a g c c g t t c c c c a a g c t a g a c c t g g g g c c g t a a g c g g c c c c t c c a g c c g t c

1380 MNPAQEAA *FRTKCGQKLHVGRLFGR a g a g g t c a g a a c c g c g t c t t g c a g c c c t g c t t c a g a t g a a c c c c g c g c a g g a a g c c g c g g t c t c c a g t c t t g g c g c a g a a c g t c g g g a c g a a g t c t a c t t g g g g c g c g t c c t t c g g c g c c

1440 AGQAGVDGGVHLAVGGGSAG RALGADVAANMQRDAPAAGT c g g g c c a g g c c g g c g t c g a c g g c g g c g t t c a t c t g g c g g t c g g c g g g g g c a g c g c c g g t g g c c c g g t c c g g c c g c a g c t g c c g c c g c a a g t a g a c c g c c a g c c g c c c c c g t c g c g g c c a c

1500 DFAHQAPERDDAVDQGLATG VERVLGRFPIISNILTKGGA a c t t c g c g c a c c a g g c c c c g g a a c g g g a t g a t g c t g t t g a t c a g g g t c t t g c c a c c g g c c t g a a g c g c g t g g t c c g g g g c c t t g c c c t a c t a c g a c a a c t a g t c c c a g a a c g g t g g c c g g

1560 LGQLAGSVFGVLGADLVVGP EALKGARNEAYEAGVKYDPG t c g g c c a g c t t g c c g g c t c g g t t t t c g g c g t a c t c g g c g c c g a c c t t g t a g t c g g g c c c c a g c c g g t c g a a c g g c c g a g c c a a a a g c c g c a t g a g c c g c g g c t g g a a c a t c a g c c c g g g g

1620 QRRVQRLHLIGQGLAGLQTR LAANLAKVENALAQCSKLGA a g c g c c g c g t t c a g c g c c t t c a c c t c a t t g g c c a g g g c c t g g c a g g a c t t c a g a c c c g c g t c g c g g c g c a a g t c g c g g a a g t g g a g t a a c c g g t c c c g g a c c g t c c t g a a g t c t g g g c g c

1680 EAVGVVKGLGQDRRHLALGQ LSYPDDFAKALVPPVERKSL a g g c t g t a g g g g t c g t c a a a g g c c t t g g c c a g g a c c g g c g g c a c c t c g c g c t t g g a c a g g t c c g a c a t c c c c a g c a g t t t c c g g a a c c g g t c c t g g c c g c c g t g g a g c g c g a a c c t g t c c

1740 ADVAHRLGADVDGLLARLAP GVDRVPQTGIDVAKKGAEGW c c g a c g t c g c g c a c c g g c t g g g t g c c g a t g t c g a c g g c c t t c t t g c c c g c c t c g c c c c a g g g c t g c a g c g c g t g g c c g a c c c a c g g c t a c a g c t g c c g g a a g a a c g g g c g g a g c g g g g t c

1800 AAGRFLGLGRRLDLGVGAGG RGSPEEPKAPPKVQAHASSA c g g c c g g a c g g t t c c t c g g g c t t g g c c g g c g g c t t g a c c t g g g c g t g g g c g c t g g a g g c c g c c g g c c t g c c a a g g a g c c c g a a c c g g c c g c c g a a c t g g a c c c g c a c c c g c g a c c t c c g g

1860 QQHGGGSGHDQQLVRHQKRP LLVAAAGAMILLKNPM a g c a g c a c g g c g g c g g c t c c g g c c a t g a t c a g c a g c t t g t t c g g c a t c a a a a g c g c c c t c t c g t c g t g c c g c c g c c g a g g c c g g t a c t a g t c g t c g a a c a a g c c g t a g t t t t c g c g g g a g

1920 PDKTRHGNGKPRRRFPPASP c c g a c a a a a c a c g c c a c g g g a a c g g c a a g c c t c g c c g c c g g t t c c c g c c c g c a a g t c c c t g g c t g t t t t g t g c g g t g c c c t t g c c g t t c g g a g c g g c g g c c a a g g g c g g g c g t t c a g g g a

1980 SARTLRPAHDPSPPQSVSSR c g g c a c g a a c t c t a a g g c c t g c g c a c g a t c c a t c g c c a c c a c a a a g c g t g t c a t c c c g g c g c c g t g c t t g a g a t t c c g g a c g c g t g c t a g g t a g c g g t g g t g t t t c g c a c a g t a g g g c c g

2040 PKRSGEPGPRATSTALRHPL c g a a g c g c a g c g g a g a g c c g g g a c c c a g g g c c a c a a g c a c g g c g c t t c g t c a c c c c c t g g g c t t c g c g t c g c c t c t c g g c c c t g g g t c c c g g t g t t c g t g c c g c g a a g c a g t g g g g g a c c

2100 GPG* g t c c c g g a t a g c g c t c c g c g c t t c c g g g a t g a c a c g g g t t t a a g c a c t t g g g t g a g a a g c c a g g g c c t a t c g c g a g g c g c g a a g g c c c t a c t g t g c c c a a a t t c g t g a a c c c a c t c t t c g

2160 *NPAGGAELDFAADFADDGP c c t a g t t c g g c g c g c c g c c c g c t t c c a g g t c g a a c g c g g c g t c g a a g g c g t c g t c g c c a g g g a t c a a g c c g c g c g g c g g g c g a a g g t c c a g c t t g c g c c g c a g c t t c c g c a g c a g c g g t c

2220 VTGELAADFLDELSSWIFAM g a a c c g t c c c t t c c a g g g c g g c g t c g a a c a g g t c t t c g a g c g a t g a c c a g a t g a a g g c c a c t t g g c a g g g a a g g t c c c g c c g c a g c t t g t c c a g a a g c t c g c t a c t g g t c t a c t t c c g g t

2280 QEMITPVSAPDFGPYLDEVL t c t g c t c c a t g a t c g t c g g g a c g c t g g c c g g a t c g a a g c c g g g g t a g a g g t c t t c c a c c a a g a c g a g g t a c t a g c a g c c c t g c g a c c g g c c t a g c t t c g g c c c c a t c t c c a g a a g g t g g t

2340 WLGTGPDFAFRCGILRFQAR g c c a c a g g c c c g t g c c g g g a t c g a a g g c g a a g c g g c a g c c g a t c a g g c g g a a c t g g g c g c c g g t g t c c g g g c a c g g c c c t a g c t t c c g c t t c g c c g t c g g c t a g t c c g c c t t g a c c c g c g

2400 AMTEFLATLDRAADLDDHDL g g g c c a t g g t c t c g a a c a g g g c c g t c a g g t c g c g g g c g g c g t c c a g g t c g t c g t g g t c c a c c c g g t a c c a g a g c t t g t c c c g g c a g t c c a g c g c c c g c c g c a g g t c c a g c a g c a c c a g g t

2460 VLCRIQLWGHDPLLYFAGED g c a c c a g g c a g c g g a t c t g c a g c c a g c c g t g g t c g g g c a g c a g g t a g a a g g c g c c c t c g t c g t g g t c c g t c g c c t a g a c g t c g g t c g g c a c c a g c c c g t c g t c c a t c t t c c g c g g g a g c a

2520 QDEGSVELGRDIAEVVYGAA c c t g a t c c t c g c c c g a a a c c t c c a g c c c c c g g t c g a t g g c t t c g a c g a c a t a g c c g g c c g g g a c t a g g a g c g g g c t t t g g a g g t c g g g g g c c a g c t a c c g a a g c t g c t g t a t c g g c c g g c

2580 RCTDTLAALLAAEQDPTLDT c g c g g c a g g t g t c g g t g a g c g c g g c c a g c a g g g c g g c t t c c t g g t c a g g g g t c a g g t c g g g c g c c g t c c a c a g c c a c t c g c g c c g g t c g t c c c g c c g a a g g a c c a g t c c c c a g t c c a g c c

2640 M t c a t g g g c a a g a g g t c c a g g t c g t g g t t t g t c g g c g g c t t c t a g c a t g g a c c g c c c g c g c a g t a c c c g t t c t c c a g g t c c a g c a c c a a a c a g c c g c c g a a g a t c g t a c c t g g c g g g c g c g

2700 MLA c c g t g a g g c c g a g g a t t t c g c g c t g g t c a g a c a a c c t a c t t g c c g t c c c c a c a t g t t a g c g g c a c t c c g g c t c c t a a a g c g c g a c c a g t c t g t t g g a t g a a c g g c a g g g g t g t a c a a t c g

PspXITth111IPflFI 2760 LPSADERAPPTVSALQTLEF *VSSN g c t a c c a a g t g c c g a c g a a c g c g c g c c g c c g a c g g t g t c g g c g c t t c a g a c g c t c g a g t t c g a t g g t t c a c g g c t g c t t g c g c g c g g c g g c t g c c a c a g c c g c g a a g t c t g c g a g c t c a a

NdeI 2820 WGDDHMADLQLQDKVSTGAP QPSSWIASKCSWSLTEVPAG t t g g g g a g a c g a c c a t a t g g c c g a c t t g c a g c t c c a g g a c a a g g t t t c g a c c g g c g c c c c a a c c c c t c t g c t g g t a t a c c g g c t g a a c g t c g a g g t c c t g t t c c a a a g c t g g c c g c g g g g

2880 RRRFDPWLVGAAVFFVAAAA RRRNSGHSTPAATNKTAAAA g c g t c g g c g g t t t g a t c c a t g g c t g g t c g g c g c g g c g g t g t t t t t c g t g g c t g c g g c g g c c g c a g c c g c c a a a c t a g g t a c c g a c c a g c c g c g c c g c c a c a a a a a g c a c c g a c g c c g c c g

FseIRsrII 2940 LSAAPALKAGPTTLAGLLLL REAAGASLAPGVVRAPRSSS c c t c t c g g c g g c g c c g g c g c t c a a g g c c g g a c c g a c c a c c c t g g c c g g c c t g c t g c t g c t g g a g a g c c g c c g c g g c c g c g a g t t c c g g c c t g g c t g g t g g g a c c g g c c g g a c g a c g a c g a

3000 LGVAGVAVLGLVAIRGSALS *RKG RPTAPTATNPRTAMRPEASE t c t g g g c g t c g c g g g c g t g g c t g t g t t g g g c c t t g t c g c c a t t c g c g g c t c a g c g c t t t c a g a c c c g c a g c g c c c g c a c c g a c a c a a c c c g g a a c a g c g g t a a g c g c c g a g t c g c g a a a g

BbvCI 3060 GGDADQAEGFIEALAEPAAL AAVGVLSLPEDLRQGLRGGQ PPSASWASPNMSASASGAAR c g g c g g c g a c g c c g a c c a g g c t g a g g g g t t c a t c g a g g c g c t g g c c g a g c c g g c c g c c c t g c c g c c g c t g c g g c t g g t c c g a c t c c c c a a g t a g c t c c g c g a c c g g c t c g g c c g g c g g g a

3120 AAADGRVLAANGPWREVMGE GGGVTADQGGVPGPAFDHAF AAASPRTRAALPGQRSTMPS g g c c g c c g c c g a c g g t c g c g t c c t g g c c g c c a a c g g g c c c t g g c g c g a a g t c a t g g g c g a c c g g c g g c g g c t g c c a g c g c a g g a c c g g c g g t t g c c c g g g a c c g c g c t t c a g t a c c c g c t

3180 QRRLPKGVAGSSLFAALVQA LAAQGLAHRAGAQKRGQDLR CRRRGLPTAPELRNAARTWA a c a g c g c c g c c t g c c c a a g g g c g t g g c g g g c t c c a g c c t g t t t g c g g c c c t g g t c c a g g c t g t c g c g g c g g a c g g g t t c c c g c a c c g c c c g a g g t c g g a c a a a c g c c g g g a c c a g g t c c g

SphI 3240 RQGQMAEGMLSAGGTDYTAK ALPLHGLAHQASSSGVIGGL RWPCIASPM g c g c c a g g g g c a g a t g g c c g a g g g c a t g c t g a g c g c t g g a g g a a c c g a c t a t a c c g c c a a c g c g g t c c c c g t c t a c c g g c t c c c g t a c g a c t c g c g a c c t c c t t g g c t g a t a t g g c g g t t

3300 VSRLAGGRLMIRLAPIVVAE DRAKRASPQHDPKRGDNDSL g g t c t c g c g c c t t g c g g g c g g a c g g c t g a t g a t c c g g c t t g c g c c c a t c g t t g t c g c t g a c c a g a g c g c g g a a c g c c c g c c t g c c g a c t a c t a g g c c g a a c g c g g g t a g c a a c a g c g a c t

AhdI 3360 PVVEDASSNVTRHRSATEMS RNHFVRRRVNRTVPRGGLHA g c c g g t t g t g g a a g a c g c g t c g t c g a a c g t t a c g c g t c a c c g g t c g g c c a c c g a g a t g a g c g g c c a a c a c c t t c t g c g c a g c a g c t t g c a a t g c g c a g t g g c c a g c c g g t g g c t c t a c t c

3420 KGEELFTGVVPILVELDGDV LALLQEGPHHGDQDLQVAVY c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t c c t g g t c g a g c t g g a c g g c g a c g t g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a g g a c c a g c t c g a c c t g c c g c t g c a

3480 NGHKFSVRGEGEGDATNGKL VAVLEADAALALAIGGVALQ a a a c g g c c a c a a g t t c a g c g t c c g c g g c g a g g g c g a g g g c g a t g c c a c c a a c g g c a a g c t t t t g c c g g t g t t c a a g t c g c a g g c g c c g c t c c c g c t c c c g c t a c g g t g g t t g c c g t t c g a

3540 TLKFICTTGKLPVPWPTLVT GQLEDAGGALQGHGPGGEHG g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c g t g c c c t g g c c c a c c c t c g t g a c c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g g c a c g g g a c c g g g t g g g a g c a c t g

3600 TFGYGVACFSRYPDHMKQHD GEAVAHGAEAAVGVVHLLVV c a c c t t c g g c t a c g g c g t g g c c t g c t t c a g c c g c t a c c c c g a c c a c a t g a a g c a g c a c g a g t g g a a g c c g a t g c c g c a c c g g a c g a a g t c g g c g a t g g g g c t g g t g t a c t t c g t c g t g c t

3660 FFKSAMPEGYVQERTISFKD EELGGHGFAVDLLAGDRELV c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g a g c g c a c c a t c t c t t t c a a g g a g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t c c t c g c g t g g t a g a g a a a g t t c c t

Acc65IKpnI 3720 DGTYKTRAEVKFEGDTLVNR VTGVLGAGLHLELAVGQHVA c g a c g g t a c c t a c a a g a c c c g c g c c g a g g t g a a g t t c g a g g g c g a c a c c c t g g t g a a c c g g c t g c c a t g g a t g t t c t g g g c g c g g c t c c a c t t c a a g c t c c c g c t g t g g g a c c a c t t g g c

3780 IELKGIDFKEDGNILGHKLE DLQLADVELLVAVDQPVLQL c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g c a a c a t c c t g g g g c a c a a g c t g g a g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c g t t g t a g g a c c c c g t g t t c g a c c t

3840 YNFNSHYVYITADKQKNCIK VVEVAVVDIDRGVLLLVADL g t a c a a c t t c a a c a g c c a c t a c g t c t a t a t c a c g g c c g a c a a g c a g a a g a a c t g c a t c a a c a t g t t g a a g t t g t c g g t g a t g c a g a t a t a g t g c c g g c t g t t c g t c t t c t t g a c g t a g t t

3900 ANFKIRHNVEDGSVQLADHY SVELDAVVNLVAAHLEGVVV g g c t a a c t t c a a g a t c c g c c a c a a c g t t g a g g a c g g c a g c g t g c a g c t c g c c g a c c a c t a c c g a t t g a a g t t c t a g g c g g t g t t g c a a c t c c t g c c g t c g c a c g t c g a g c g g c t g g t g a t

3960 QQNTPIGDGPVLLPDNHYLS LLVGGDAVAGHQQGVVVVQA c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g c t g c c c g a c a a c c a c t a c c t g a g g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a c g a c g g g c t g t t g g t g a t g g a c t c

4020 C A T G G T C C T G C T G G A EGFP-C HQSKLSKDPNEKRDHMVLLE MLGLQAFVGVLLAIVHDQQL c c a t c a g t c c a a g c t g a g c a a a g a c c c c a a c g a g a a g c g c g a t c a c a t g g t c c t g c t g g a g g t a g t c a g g t t c g a c t c g t t t c t g g g g t t g c t c t t c g c g c t a g t g t a c c a g g a c g a c c t

4080 G T T C G T G EGFP-C FVTAALESGGEDPMVSPAPV EHGGGQFAAAFIRHHARGRD g t t c g t g a c c g c c g c c c t g g a a a g c g g c g g c g a a g a t c c g a t g g t g a g c c c g g c c c c g g t c a a g c a c t g g c g g c g g g a c c t t t c g c c g c c g c t t c t a g g c t a c c a c t c g g g c c g g g g c c a

4140 AERAAPPPSSLDAFAGASPF GLAGGRWGARQVGEGSGRRE c g c c g a g c g c g c c g c c c c g c c a c c c a g c t c g c t g g a c g c c t t c g c c g g a g c c t c g c c g t t g c g g c t c g c g c g g c g g g g c g g t g g g t c g a g c g a c c t g c g g a a g c g g c c t c g g a g c g g c a a

4200 GAALLEGLEPFTSRVLETNP ARGQQFAQLREGRPHQLGIR c g g c g c g g c c c t g c t g g a a g g c c t g g a g c c g t t c a c c t c g c g g g t g c t g g a g a c c a a t c c g c c g c g c c g g g a c g a c c t t c c g g a c c t c g g c a a g t g g a g c g c c c a c g a c c t c t g g t t a g g

4260 ALTTMTGAKAGVLFGDLIDA RQGRHGAGLGTHQEAIQDVG g g c g c t g a c c a c g a t g a c c g g c g c c a a g g c c g g t g t g c t g t t c g g c g a t c t g a t c g a c g c c c g c g a c t g g t g c t a c t g g c c g c g g t t c c g g c c a c a c g a c a a g c c g c t a g a c t a g c t g c g

4320 ASRAEAETRLNEGRAGPYEV GRAGLGLRAQVFAAGTGVLH c g c c t c g c g c g c c g a g g c c g a g a c g c g c c t g a a c g a a g g c c g c g c c g g t c c c t a c g a g g t g c g g a g c g c g c g g c t c c g g c t c t g c g c g g a c t t g c t t c c g g c g c g g c c a g g g a t g c t c c a

4380 RLARDPSRIAHLYLYRAEGR PQRAIRRADSVKVEIAGFAA g c g g t t g g c g c g c g a t c c g t c g c g c a t c g c t c a c c t t t a c c t c t a t c g c g c c g a a g g c c g c g c c a a c c g c g c g c t a g g c a g c g c g t a g c g a g t g g a a a t g g a g a t a g c g c g g c t t c c g g c

4440 LVAYMIAVSEQKQIELQLSQ QHGVHDGHGLLLLDLQLQGL c c t g g t g g c c t a c a t g a t c g c c g t g t c c g a g c a g a a g c a g a t c g a g c t g c a g c t g t c c c a g g a c c a c c g g a t g t a c t a g c g g c a c a g g c t c g t c t t c g t c t a g c t c g a c g t c g a c a g g g t

4500 AQKMQAIGQLAGGVAHDFNN GLLHLGDALQGAADRVVEVV g g c c c a g a a g a t g c a g g c c a t c g g c c a g c t g g c c g g c g g c g t c g c g c a c g a c t t c a a c a a c c g g g t c t t c t a c g t c c g g t a g c c g g t c g a c c g g c c g c c g c a g c g c g t g c t g a a g t t g t t

4560 LLTAIQLRLDELLHRHPVGD EQGGDLQTQVLQQMAM c c t c t t g a c c g c c a t c c a g c t g c g t c t g g a c g a g t t g c t g c a t c g c c a t c c c g t c g g t g a g g a g a a c t g g c g g t a g g t c g a c g c a g a c c t g c t c a a c g a c g t a g c g g t a g g g c a g c c a c t

4620 PSYEGLNEIRQTGVRAADLV t c c g t c g t a c g a g g g t c t c a a c g a g a t c c g t c a g a c g g g c g t g c g c g c c g c c g a c c t c g t a g g c a g c a t g c t c c c a g a g t t g c t c t a g g c a g t c t g c c c g c a c g c g c g g c g g c t g g a g c a

4680 RKLLAFSRKQTVQREVLDLG g c g c a a g c t c t t g g c t t t c t c g c g c a a g c a g a c c g t g c a g c g c g a g g t g c t g g a t c t g g g c g c g t t c g a g a a c c g a a a g a g c g c g t t c g t c t g g c a c g t c g c g c t c c a c g a c c t a g a c c c

4740 ELISEFEVLLRRLLREDVKL c g a g c t g a t c a g c g a g t t c g a g g t c t t g c t g c g c c g c c t g c t g c g c g a a g a c g t c a a g c t g c t c g a c t a g t c g c t c a a g c t c c a g a a c g a c g c g g c g g a c g a c g c g c t t c t g c a g t t c g a

4800 ITDYGRDLPQVRADKSQLET g a t c a c c g a c t a t g g c c g c g a c c t g c c g c a g g t g c g c g c c g a c a a g a g c c a g c t c g a g a c c t a g t g g c t g a t a c c g g c g c t g g a c g g c g t c c a c g c g c g g c t g t t c t c g g t c g a g c t c t g

4860 AVMNLAVNARDAVRAAKGGG g g c g g t c a t g a a c c t g g c c g t c a a c g c c c g c g a c g c c g t g c g c g c g g c c a a g g g c g g c g g c c g c c a g t a c t t g g a c c g g c a g t t g c g g g c g c t g c g g c a c g c g c g c c g g t t c c c g c c g c c

FspAI 4920 VVRIRTARLTRDEAIQLGFP c g t c g t g c g c a t c c g c a c c g c g c g c c t g a c c c g c g a c g a g g c g a t c c a g c t g g g t t t c c c g c a g c a c g c g t a g g c g t g g c g c g c g g a c t g g g c g c t g c t c c g c t a g g t c g a c c c a a a g g g

4980 AADGDTAFIEVSDDGPGIPP g g c c g c c g a c g g c g a c a c g g c c t t c a t t g a g g t c a g t g a c g a t g g t c c g g g c a t t c c g c c c c g g c g g c t g c c g c t g t g c c g g a a g t a a c t c c a g t c a c t g c t a c c a g g c c c g t a a g g c g g

BglII 5040 DVMGKIFDPFFTTKPVGEGT c g a c g t c a t g g g c a a g a t c t t c g a c c c g t t c t t c a c c a c c a a g c c g g t g g g c g a g g g t a c g c t g c a g t a c c c g t t c t a g a a g c t g g g c a a g a a g t g g t g g t t c g g c c a c c c g c t c c c a t g

5100 GLGLATVYGIVKQSDGWIHV *GRDIADNLLAVAPNVD g g g c c t a g g c c t a g c c a c g g t c t a t g g c a t c g t t a a g c a g a g c g a c g g c t g g a t t c a c g t c c c g g a t c c g g a t c g g t g c c a g a t a c c g t a g c a a t t c g t c t c g c t g c c g a c c t a a g t g c a

5160 HSRPNEGAAFRIFLPVYEAP VATRVFARGEADEQRDIFRG c c a c a g c c g t c c g a a c g a a g g c g c g g c c t t c c g c a t c t t c c t g c c g g t c t a t g a a g c g c c g g t g t c g g c a g g c t t g c t t c c g c g c c g g a a g g c g t a g a a g g a c g g c c a g a t a c t t c g c g g

NruI 5220 AGAVAVQAVAEPAKPRAARD GARDGDLGDGLGGLRAGSAV c g c c g g c g c g g t c g c c g t c c a g g c c g t c g c c g a g c c c g c c a a g c c g c g c g c c g c t c g c g a g c g g c c g c g c c a g c g g c a g g t c c g g c a g c g g c t c g g g c g g t t c g g c g c g c g g c g a g c g c t

5280 LSGAGRILFVEDEDAVRSVA QRAGAADQEDLVLVGHAADG c c t g t c g g g c g c c g g c c g c a t c c t g t t c g t c g a g g a c g a g g a c g c c g t g c g c a g c g t c g c g g a c a g c c c g c g g c c g g c g t a g g a c a a g c a g c t c c t g c t c c t g c g g c a c g c g t c g c a g c g

5340 ARLLRARGYEVLEAADGEEA GAQQAGTAVLHKLRGVAFLG c g c c c g c c t g c t g c g c g c c c g t g g c t a c g a g g t g c t t g a g g c g g c c g a c g g c g a a g a g g c g c g g g c g g a c g a c g c g c g g g c a c c g a t g c t c c a c g a a c t c c g c c g g c t g c c g c t t c t c c g

5400 LIIAEENAGTIDLLISDVIM QDDGLFVGARDVEQDAVHDH c c t g a t c a t c g c c g a g g a a a a c g c c g g c a c g a t c g a c c t c t t g a t c a g c g a c g t g a t c a t g g a c t a g t a g c g g c t c c t t t t g c g g c c g t g c t a g c t g g a g a a c t a g t c g c t g c a c t a g t a

5460 PGIDGPTLLKKARGYLGTAP GADVARGQQLLRTAIQPGRR g c c c g g c a t c g a c g g c c c g a c c t t g c t g a a g a a g g c g c g t g g c t a t c t g g g g a c c g c g c c c g g g c c g t a g c t g c c g g g c t g g a a c g a c t t c t t c c g c g c a c c g a t a g a c c c c t g g c g c g g

5520 VMFISGYAEAEFSDLLEGET HHEDGAIGLGLEAVEQFALR g g t g a t g t t c a t c t c c g g c t a t g c c g a g g c c g a g t t c a g c g a c c t c t t g g a a g g c g a g a c c c a c t a c a a g t a g a g g c c g a t a c g g c t c c g g c t c a a g t c g c t g g a g a a c c t t c c g c t c t g

5580 GVTFLPKPIDIKTLAERVKQ AHGEEGLRDVDLGQGLADLL g g g c g t g a c c t t c c t c c c c a a g c c g a t c g a c a t c a a g a c c c t g g c c g a g c g c g t c a a g c a c c c g c a c t g g a a g g a g g g g t t c g g c t a g c t g t a g t t c t g g g a c c g g c t c g c g c a g t t c g t

BspQISapI 5640 5 SKGEE mRuby3 QLQAASNVTRHRSATSKGEE LQLRRRVNRTVPRGGRLAFL g c a g c t g c a g g c g g c g t c g a a c g t t a c g c g t c a c c g g t c g g c c a c c t c t a a g g g c g a a g a c g t c g a c g t c c g c c g c a g c t t g c a a t g c g c a g t g g c c a g c c g g t g g a g a t t c c c g c t t c t

5700 10 15 20 25 LIKENMRMKVVMEGSVNGHQ mRuby3 LIKENMRMKVVMEGSVNGHQ QDLFIHTHLHDHFTRDVAVL g c t g a t c a a g g a a a a t a t g c g t a t g a a g g t g g t c a t g g a a g g t t c g g t c a a c g g c c a c c a c g a c t a g t t c c t t t t a t a c g c a t a c t t c c a c c a g t a c c t t c c a a g c c a g t t g c c g g t g g t

5760 30 35 40 45 FKCTGEGEGRPYEGVQTMRI mRuby3 FKCTGEGEGRPYEGVQTMRI EFACTFSFASRVLSHLGHPD a t t c a a a t g c a c a g g t g a a g g a g a a g g c a g a c c g t a c g a g g g a g t g c a a a c c a t g a g g a t t a a g t t t a c g t g t c c a c t t c c t c t t c c g t c t g g c a t g c t c c c t c a c g t t t g g t a c t c c t a

5820 50 55 60 65 KVIEGGPLPFAFDILATSFM mRuby3 KVIEGGPLPFAFDILATSFM FDDLSSGQWKGKVNKGRREH c a a a g t c a t c g a g g g a g g a c c c c t g c c a t t t g c c t t t g a c a t t c t t g c c a c g t c g t t c a t g t t t c a g t a g c t c c c t c c t g g g g a c g g t a a a c g g a a a c t g t a a g a a c g g t g c a g c a a g t a

5880 70 75 80 85 YGSRTFIKYPADIPDFFKQS mRuby3 YGSRTFIKYPADIPDFFKQS IAATGKDLVRGVDRIEKFLG g t a t g g c a g c c g t a c c t t t a t c a a g t a c c c g g c c g a c a t c c c t g a t t t c t t t a a a c a g t c c a t a c c g t c g g c a t g g a a a t a g t t c a t g g g c c g g c t g t a g g g a c t a a a g a a a t t t g t c a g

5940 90 95 100 105 FPEGFTWERVTRYEDGGVVT mRuby3 FPEGFTWERVTRYEDGGVVT KRLTKSPFSNRSVFITSDDG c t t t c c t g a g g g t t t t a c t t g g g a a a g a g t t a c g a g a t a c g a a g a t g g t g g a g t c g t c a c g a a a g g a c t c c c a a a a t g a a c c c t t t c t c a a t g c t c t a t g c t t c t a c c a c c t c a g c a g t g

Eco53kISacI 6000 110 115 120 125 VTQDTSLEDGELVYNVKVRG mRuby3 VTQDTSLEDGELVYNVKVRG DRLVGAKLIALEDVVDLDSP c g t c a c g c a g g a c a c c a g c c t t g a g g a t g g c g a g c t c g t c t a c a a c g t c a a g g t c a g a g g g c a g t g c g t c c t g t g g t c g g a a c t c c t a c c g c t c g a g c a g a t g t t g c a g t t c c a g t c t c c

6060 130 135 140 145 VNFPSNGPVMQKKTKGWEPN mRuby3 VNFPSNGPVMQKKTKGWEPN YVKGGITGHHLLLGLTPLRI g g t a a a c t t t c c c t c c a a t g g t c c c g t g a t g c a g a a g a a g a c c a a g g g t t g g g a g c c t a a c c a t t t g a a a g g g a g g t t a c c a g g g c a c t a c g t c t t c t t c t g g t t c c c a a c c c t c g g a t t

6120 150 155 160 165 TEMMYPADGGLRGYTDIALK mRuby3 TEMMYPADGGLRGYTDIALK CLHHIWCITTQSSVSVDCQF t a c a g a g a t g a t g t a t c c a g c a g a t g g t g g t c t g a g a g g a t a c a c t g a c a t c g c a c t g a a a t g t c t c t a c t a c a t a g g t c g t c t a c c a c c a g a c t c t c c t a t g t g a c t g t a g c g t g a c t t

6180 170 175 180 185 VDGGGHLHCNFVTTYRSKKT mRuby3 VDGGGHLHCNFVTTYRSKKT NITTAM a g t t g a t g g t g g t g g c c a t c t g c a c t g c a a c t t c g t g a c a a c t t a c a g g t c a a a a a a g a c t c a a c t a c c a c c a c c g g t a g a c g t g a c g t t g a a g c a c t g t t g a a t g t c c a g t t t t t t c t g

6240 190 195 200 205 VGNIKMPGVHAIDHRLERIE mRuby3 VGNIKMPGVHAIDHRLERIE c g t c g g g a a c a t c a a g a t g c c c g g t g t c c a t g c c a t t g a t c a c c g c c t g g a a a g g a t c g a g c a g c c c t t g t a g t t c t a c g g g c c a c a g g t a c g g t a a c t a g t g g c g g a c c t t t c c t a g c t

SnaBIBsaAI 6300 210 215 220 225 ESDNETYVVQREVAVAKYSN mRuby3 ESDNETYVVQREVAVAKYSN g g a g a g t g a c a a t g a a a c c t a c g t a g t g c a a a g a g a a g t g g c a g t t g c c a a a t a c a g c a a c c t c t c a c t g t t a c t t t g g a t g c a t c a c g t t t c t c t t c a c c g t c a a c g g t t t a t g t c g t t

TspMIXmaISmaIBsrGI 6360 230 235 LGGGMDELYK * mRuby3 SK primer LGGGMDELYK* c c t t g g t g g t g g c a t g g a c g a g c t g t a c a a g t a a a g c t g c a g c c c g g g g g a t c c a c t a g t g g a a c c a c c a c c g t a c c t g c t c g a c a t g t t c a t t t c g a c g t c g g g c c c c c t a g g t g a t c a

6420 SK primer M13 fwd T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward t c t a g a g c g g c c a t t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g a g a t c t c g c c g g t a a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c

6480 t t a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c g g t a t c a g c t c a c t c a a a t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g c c a t a g t c g a g t g a g t

6540 a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a c a t g t g a g c a t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t g t a c a c t c g t

6600 ori a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t t t t c c a t a g g t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a a a a g g t a t c c

6660 ori c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g g c g a a a c c c g g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c c g c t t t g g g c

6720 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c t c t c c t g t t t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c g a g a g g a c a a

6780 ori c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g c g t g g c g c t t g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c g c a c c g c g a a

6840 ori t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c c a a g c t g g g c a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g g t t c g a c c c g

ApaLI 6900 ori t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a c t a t c g t c t t a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t g a t a g c a g a a

6960 ori g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g t a a c a g g a t t c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c a t t g t c c t a a

7020 ori a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c t a a c t a c g g c t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g a t t g a t g c c g

7080 ori t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c c t t c g g a a a a a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g g a a g c c t t t t

7140 ori a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g t t t t t t t g t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c a a a a a a a c a a

7200 ori t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t g a t c t t t t c t a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a c t a g a a a a g a

7260 a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c a t c g a a c c c t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a g t a g c t t g g g

7320 c a g a g t c c c g g c t a g a t c a c g c a a c t g g t c c a g a a c c t t g a c c g a a c g c a g c g g t g g t a a g t c t c a g g g c c g a t c t a g t g c g t t g a c c a g g t c t t g g a a c t g g c t t g c g t c g c c a c c a t t

7380 c g g c g c a g t g g c g g t t t t c a t g g c t t g t t a t g a c t g t t t t t t t g g g t a c a g t c t a t g c c t g c c g c g t c a c c g c c a a a a g t a c c g a a c a a t a c t g a c a a a a a a a c c c a t g t c a g a t a c g g a

7440 c g g g c a t c c a a g c a g c a a g c g c g t t a c g c c g t g g g t c g a t g t t t g a t g t t a t g g a g c a g c g c c c g t a g g t t c g t c g t t c g c g c a a t g c g g c a c c c a g c t a c a a a c t a c a a t a c c t c g t c g

7500 a a c g a t g t t a c g c a g c a g g g c a g t c g c c c t a a a a c a a a g t t a a a c a t c a t g a g g a g g t a a t t g c t a c a a t g c g t c g t c c c g t c a g c g g g a t t t t g t t t c a a t t t g t a g t a c t c c t c c a t t

7560 1 5 10 15 MEKKITGYTTVDISQWHRK CmR MEKKITGYTTVDISQWHRK c g t c a t g g a g a a a a a a a t c a c t g g a t a t a c c a c c g t t g a t a t a t c c c a a t g g c a t c g t a a g c a g t a c c t c t t t t t t t a g t g a c c t a t a t g g t g g c a a c t a t a t a g g g t t a c c g t a g c a t t

7620 20 25 30 35 EHFEAFQSVAQCTYNQTVQL CmR C T C C G T A A A G T C A G T C A A C G CAT-R EHFEAFQSVAQCTYNQTVQL a g a a c a t t t t g a g g c a t t t c a g t c a g t t g c t c a a t g t a c c t a t a a c c a g a c c g t t c a g c t t c t t g t a a a a c t c c g t a a a g t c a g t c a a c g a g t t a c a t g g a t a t t g g t c t g g c a a g t c g a

7680 40 45 50 55 DITAFLKTVKKNKHKFYPAF CmR DITAFLKTVKKNKHKFYPAF g g a t a t t a c g g c c t t t t t a a a g a c c g t a a a g a a a a a t a a g c a c a a g t t t t a t c c g g c c t t c c t a t a a t g c c g g a a a a a t t t c t g g c a t t t c t t t t t a t t c g t g t t c a a a a t a g g c c g g a a

EcoRIApoI 7740 60 65 70 75 IHILARLMNAHPEFRMAMKD CmR IHILARLMNAHPEFRMAMKD t a t t c a c a t t c t t g c c c g c c t g a t g a a t g c t c a t c c g g a a t t c c g t a t g g c a a t g a a a g a a t a a g t g t a a g a a c g g g c g g a c t a c t t a c g a g t a g g c c t t a a g g c a t a c c g t t a c t t t c t

7800 80 85 90 95 GELVIWDSVHPCYTVFHEQT CmR GELVIWDSVHPCYTVFHEQT c g g t g a g c t g g t g a t a t g g g a t a g t g t t c a c c c t t g t t a c a c c g t t t t c c a t g a g c a a a c g c c a c t c g a c c a c t a t a c c c t a t c a c a a g t g g g a a c a a t g t g g c a a a a g g t a c t c g t t t g

7860 100 105 110 115 ETFSSLWSEYHDDFRQFLHI CmR ETFSSLWSEYHDDFRQFLHI t g a a a c g t t t t c a t c g c t c t g g a g t g a a t a c c a c g a c g a t t t c c g g c a g t t t c t a c a c a t a c t t t g c a a a a g t a g c g a g a c c t c a c t t a t g g t g c t g c t a a a g g c c g t c a a a g a t g t g t a

7920 120 125 130 135 YSQDVACYGENLAYFPKGFI CmR YSQDVACYGENLAYFPKGFI a t a t t c g c a a g a t g t g g c g t g t t a c g g t g a a a a c c t g g c c t a t t t c c c t a a a g g g t t t a t t a t a a g c g t t c t a c a c c g c a c a a t g c c a c t t t t g g a c c g g a t a a a g g g a t t t c c c a a a t a

7980 140 145 150 155 ENMFFVSANPWVSFTSFDLN CmR ENMFFVSANPWVSFTSFDLN t g a g a a t a t g t t t t t c g t c t c a g c c a a t c c c t g g g t g a g t t t c a c c a g t t t t g a t t t a a a a c t c t t a t a c a a a a a g c a g a g t c g g t t a g g g a c c c a c t c a a a g t g g t c a a a a c t a a a t t t

SspI 8040 160 165 170 175 VANMDNFFAPVFTMGKYYTQ CmR VANMDNFFAPVFTMGKYYTQ c g t g g c c a a t a t g g a c a a c t t c t t c g c c c c c g t t t t c a c c a t g g g c a a a t a t t a t a c g c a g c a c c g g t t a t a c c t g t t g a a g a a g c g g g g g c a a a a g t g g t a c c c g t t t a t a a t a t g c g t

8100 180 185 190 195 GDKVLMPLAIQVHHAVCDGF CmR GDKVLMPLAIQVHHAVCDGF a g g c g a c a a g g t g c t g a t g c c g c t g g c g a t t c a g g t t c a t c a t g c c g t t t g t g a t g g c t t t c c g c t g t t c c a c g a c t a c g g c g a c c g c t a a g t c c a a g t a g t a c g g c a a a c a c t a c c g a a

ScaI 8160 200 205 210 215 HVGRMLNELQQYCDEWQGGA CmR HVGRMLNELQQYCDEWQGGA c c a t g t c g g c a g a a t g c t t a a t g a a t t a c a a c a g t a c t g c g a t g a g t g g c a g g g c g g g g c g g t a c a g c c g t c t t a c g a a t t a c t t a a t g t t g t c a t g a c g c t a c t c a c c g t c c c g c c c c g

8220 220 * CmR * g t a a c c t a g g c a t g a t g a t a t a t t t t t a t c t t g t g c a a t g t a a c a t c a g a g a t t t t g a g a c a t t g g a t c c g t a c t a c t a t a t a a a a a t a g a a c a c g t t a c a t t g t a g t c t c t a a a a c t c t

NotI 8265 c a c a a c g t g g c t t t c c c c c c c c c c c c c a c c g c g g t g g c g g c c g c c g t g t t g c a c c g a a a g g g g g g g g g g g g g t g g c g c c a c c g c c g g c g g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.