Addgene R713-M85-303: CMV51p> Halotag-Hs.KRAS4a Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: R713-M85-303: CMV51p> Halotag-Hs.KRAS4a Sequencing Result

Map View

SgrDI (8894) SspI (8777) Amp-R (8523 .. 8542) ScaI (8453) pBR322ori-F (7221 .. 7240) L4440 (6970 .. 6987) CAP binding site lac promoter M13/pUC Reverse (6752 .. 6774) lac operator M13 Reverse (6739 .. 6755) M13 rev EBV-rev (6660 .. 6679) BsmI (6649) SV40pA-R (6606 .. 6625) PfoI (6504) BstBI (6411) Neo-F (6265 .. 6284) RsrII (6245) PluTI (5732) SfoI (5730) NarI (5729) KasI (5728) Neo-R (5655 .. 5674) SV40pro-F (5447 .. 5466) CsiI - SexAI * (5286) pBABE 3' (5200 .. 5220) F1ori-F (5060 .. 5081) DraIII (4996) F1ori-R (4850 .. 4869) BGH-rev (4487 .. 4504) SP6 (4450 .. 4467) SP6 promoter HpaI (196) PpuMI (318) Bsu36I (322) SpeI (658) MluI (1012) XcmI (1916) pRS-marker (2030 .. 2049) MfeI (2147) attB4 SbfI (2227) SnaBI (2509) CMV-F (2688 .. 2708) LNCX (2734 .. 2758) BsmBI - Esp3I (2758) AleI (2928) BstEII (2946) BmgBI (3080) BspDI * - ClaI * (3316) PshAI (3375) SgrAI (3462) TEV site AsiSI (3763) EcoRI (4207) attB2 NotI (4416) PspXI (4422) XbaI (4434) R713-M85-303: CMV51p> Halotag-Hs.KRAS4a 8896 bp

Sequence View

5ʹ 3ʹ 60 g a c g g a t c g g g a g a t c c g c c t t t a t g t g t a a c t c t t g g c t g a a g c t c t t a c a c c a a t g c t c t g c c t a g c c c t c t a g g c g g a a a t a c a c a t t g a g a a c c g a c t t c g a g a a t g t g g t t a c g a

120 g g g g g a c a t g t a c c t c c c a g g g g c c c a g g a a g a c t a c g g g a g g c t a c a c c a a c g t c a a t c c c c c c t g t a c a t g g a g g g t c c c c g g g t c c t t c t g a t g c c c t c c g a t g t g g t t g c a g t t a g

180 a g a g g g g c c t g t g t a g c t a c c g a t a a g c g g a c c c t c a a g a g g g c a t t a g c a a t a g t g t t t t c t c c c c g g a c a c a t c g a t g g c t a t t c g c c t g g g a g t t c t c c c g t a a t c g t t a t c a c a a a

HpaI 240 oriP a t a a g g c c c c c t t g t t a a c c c t a a a c g g g t a g c a t a t g c t t c c c g g g t a g t a g t a t a t a c t a t t c c g g g g g a a c a a t t g g g a t t t g c c c a t c g t a t a c g a a g g g c c c a t c a t c a t a t a t g

300 oriP t a t c c a g a c t a a c c c t a a t t c a a t a g c a t a t g t t a c c c a a c g g g a a g c a t a t g c t a t c g a a t a g g t c t g a t t g g g a t t a a g t t a t c g t a t a c a a t g g g t t g c c c t t c g t a t a c g a t a g c t

PpuMIBsu36I 360 oriP a t t a g g g t t a g t a a a a g g g t c c t a a g g a a c a g c g a t a t c t c c c a c c c c a t g a g c t g t c a c t a a t c c c a a t c a t t t t c c c a g g a t t c c t t g t c g c t a t a g a g g g t g g g g t a c t c g a c a g t g

420 oriP g g t t t t a t t t a c a t g g g g t c a g g a t t c c a c g a g g g t a g t g a a c c a t t t t a g t c a c a a g g g c c a a a a t a a a t g t a c c c c a g t c c t a a g g t g c t c c c a t c a c t t g g t a a a a t c a g t g t t c c c

480 oriP c a g t g g c t g a a g a t c a a g g a g c g g g c a g t g a a c t c t c c t g a a t c t t c g c c t g c t t c t t c a g t c a c c g a c t t c t a g t t c c t c g c c c g t c a c t t g a g a g g a c t t a g a a g c g g a c g a a g a a g t

540 oriP t t c t c c t t c g t t t a g c t a a t a g a a t a a c t g c t g a g t t g t g a a c a g t a a g g t g t a t g t g a g a a g a g g a a g c a a a t c g a t t a t c t t a t t g a c g a c t c a a c a c t t g t c a t t c c a c a t a c a c t c

600 oriP g t g c t c g a a a a c a a g g t t t c a g g t g a c g c c c c c a g a a t a a a a t t t g g a c g g g g g g t t c a g c a c g a g c t t t t g t t c c a a a g t c c a c t g c g g g g g t c t t a t t t t a a a c c t g c c c c c c a a g t c

SpeI 660 oriP t g g t g g c a t t g t g c t a t g a c a c c a a t a t a a c c c t c a c a a a c c c c t t g g g c a a t a a a t a c t a c c a c c g t a a c a c g a t a c t g t g g t t a t a t t g g g a g t g t t t g g g g a a c c c g t t a t t t a t g a

720 oriP a g t g t a g g a a t g a a a c a t t c t g a a t a t c t t t a a c a a t a g a a a t c c a t g g g g t g g g g a c a a t c a c a t c c t t a c t t t g t a a g a c t t a t a g a a a t t g t t a t c t t t a g g t a c c c c a c c c c t g t t

780 oriP g c c g t a a a g a c t g g a t g t c c a t c t c a c a c g a a t t t a t g g c t a t g g g c a a c a c a t a a t c c t c g g c a t t t c t g a c c t a c a g g t a g a g t g t g c t t a a a t a c c g a t a c c c g t t g t g t a t t a g g a

840 oriP a g t g c a a t a t g a t a c t g g g g t t a t t a a g a t g t g t c c c a g g c a g g g a c c a a g a c a g g t g a a t c a c g t t a t a c t a t g a c c c c a a t a a t t c t a c a c a g g g t c c g t c c c t g g t t c t g t c c a c t t

900 oriP c c a t g t t g t t a c a c t c t a t t t g t a a c a a g g g g a a a g a g a g t g g a c g c c g a c a g c a g c g g a g g t a c a a c a a t g t g a g a t a a a c a t t g t t c c c c t t t c t c t c a c c t g c g g c t g t c g t c g c c t

960 oriP c t c c a c t g g t t g t c t c t a a c a c c c c c g a a a a t t a a a c g g g g c t c c a c g c c a a t g g g g c c c g a g g t g a c c a a c a g a g a t t g t g g g g g c t t t t a a t t t g c c c c g a g g t g c g g t t a c c c c g g g

MluI 1020 oriP a t a a a c a a a g a c a a g t g g c c a c t c t t t t t t t t g a a a t t g t g g a g t g g g g g c a c g c g t c a g t a t t t g t t t c t g t t c a c c g g t g a g a a a a a a a a c t t t a a c a c c t c a c c c c c g t g c g c a g t c

1080 oriP c c c c c a c a c g c c g c c c t g c g g t t t t g g a c t g t a a a a t a a g g g t g t a a t a a c t t g g c t g a t g g g g g t g t g c g g c g g g a c g c c a a a a c c t g a c a t t t t a t t c c c a c a t t a t t g a a c c g a c t a

1140 oriP t g t a a c c c c g c t a a c c a c t g c g g t c a a a c c a c t t g c c c a c a a a a c c a c t a a t g g c a c c c c a c a t t g g g g c g a t t g g t g a c g c c a g t t t g g t g a a c g g g t g t t t t g g t g a t t a c c g t g g g g

1200 oriP g g g g a a t a c c t g c a t a a g t a g g t g g g c g g g c c a a g a t a g g g g c g c g a t t g c t g c g a t c t g c c c c t t a t g g a c g t a t t c a t c c a c c c g c c c g g t t c t a t c c c c g c g c t a a c g a c g c t a g a c

1260 oriP g a g g a c a a a t t a c a c a c a c t t g c g c c t g a g c g c c a a g c a c a g g g t t g t t g g t c c t c a t a t c t c c t g t t t a a t g t g t g t g a a c g c g g a c t c g c g g t t c g t g t c c c a a c a a c c a g g a g t a t a

1320 oriP t c a c g a g g t c g c t g a g a g c a c g g t g g g c t a a t g t t g c c a t g g g t a g c a t a t a c t a c c c a a a g t g c t c c a g c g a c t c t c g t g c c a c c c g a t t a c a a c g g t a c c c a t c g t a t a t g a t g g g t t

1380 oriP a t a t c t g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t t a t a g a c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a

1440 oriP a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t t t t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a a a

1500 oriP a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a

1560 oriP a t a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t c c g g g t a g c a t a t g c t a t c c t a a t a g t a t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a g g c c c a t c g t a t a c g a t a g g a t t a t c

1620 oriP a g a t t a g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a t a c t a c c c a a a t a t t c t a a t c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t a t g a t g g g t t t a t a

1680 oriP c t g g a t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t g a c c t a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a

1740 oriP c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a

1800 oriP c t g g g t a g t a t a t g c t a t c c t a a t t t a t a t c t g g g t a g c a t a g g c t a t c c t a a t c t a t a t g a c c c a t c a t a t a c g a t a g g a t t a a a t a t a g a c c c a t c g t a t c c g a t a g g a t t a g a t a t a

1860 oriP c t g g g t a g c a t a t g c t a t c c t a a t c t a t a t c t g g g t a g t a t a t g c t a t c c t a a t c t g t a t g a c c c a t c g t a t a c g a t a g g a t t a g a t a t a g a c c c a t c a t a t a c g a t a g g a t t a g a c a t a

XcmI 1920 oriP c c g g g t a g c a t a t g c t a t c c t c a t g c a t a t a c a g t c a g c a t a t g a t a c c c a g t a g t a g a g g g c c c a t c g t a t a c g a t a g g a g t a c g t a t a t g t c a g t c g t a t a c t a t g g g t c a t c a t c t c

1980 oriP t g g g a g t g c t a t c c t t t g c a t a t g c c g c c a c c t c c c a a g g g g g c g t g a a t t t t c g c t g c t a c c c t c a c g a t a g g a a a c g t a t a c g g c g g t g g a g g g t t c c c c c g c a c t t a a a a g c g a c g a

2040 oriP A T G T T A G A C G A pRS-marker t g t c c t t t t c c t g c t g g g g a t c t c c c g a t c c c c t a t g g t g c a c t c t c a g t a c a a t c t g c t a c a g g a a a a g g a c g a c c c c t a g a g g g c t a g g g g a t a c c a c g t g a g a g t c a t g t t a g a c g a

2100 G A C T A C G G C pRS-marker c t g a t g c c g c a t a g t t a a g c c a g t a t c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t g a c t a c g g c g t a t c a a t t c g g t c a t a g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a

MfeI 2160 a g t g c g c g a g c a a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a t c a c g c g c t c g t t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t

2220 attB4 a t c t g c t t a g g g t t a g g c g t t t t g c g c t g c t t c g a t c a c a a c t t t g t a t a g a a a a g t t g g t a g a c g a a t c c c a a t c c g c a a a a c g c g a c g a a g c t a g t g t t g a a a c a t a t c t t t t c a a c c

SbfI 2280 CMV enhancer c c c t g c a g g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c g g g a c g t c c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g

2340 CMV enhancer c c g c c c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a g g c g g g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t

2400 CMV enhancer t t g a c g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a a a c t g c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t

2460 CMV enhancer t c a t a t g c c a a g t c c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a a g t a t a c g g t t c a g g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t

SnaBI 2520 CMV enhancer t g c c c a g t a c a t g a c c t t a c g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t a c g g g t c a t g t a c t g g a a t g c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a

2580 CMV enhancer CMV promoter c g c t a t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a c c a a t g g g c g t g g a t a g c g g t t t g a g c g a t a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t g g t t a c c c g c a c c t a t c g c c a a a c t

2640 CMV promoter *HSNTKAG c t c a c g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a g a g t g c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t

2700 CMV promoter C G C A A A T G G G C G G CMV-F FDVPSELIDYYGRGTSAFPR a a a t c a a c g g g a c t t t c c a a a a t g t c g t a a t a a c c c c g c c c c g t t g a c g c a a a t g g g c g g t t t a g t t g c c c t g a a a g g t t t t a c a g c a t t a t t g g g g c g g g g c a a c t g c g t t t a c c c g c c

BsmBIEsp3I 2760 CMV promoter T A G G C G T G CMV-F A G C T C G T T T A G T G A A C C G T C A G A T C LNCX YAHVTPPRYLCLENLSGDSR t a g g c g t g t a c g g t g g g a g g t c t a t a t a a g c a g a g c t c g t t t a g t g a a c c g t c a g a t c g c a t c c g c a c a t g c c a c c c t c c a g a t a t a t t c g t c t c g a g c a a a t c a c t t g g c a g t c t a g c g

2820 attB5 RSVGDVSNQGGYFVALKQIC c t g g a g a c g c c a t c c a c g c t g t t t t g a c c t c c a t a g a a g a c a g c c a a c t t t t g t a t a c a a g a c c t c t g c g g t a g g t g c g a c a a a a c t g g a g g t a t c t t c t g t c g g t t g a a a a c a t a t g t t

2880 1 5 10 15 MAEIGTGFPFDPHYVEV attB5 HaloTag® MAEIGTGFPFDPHYVEV LPGHCFDTSAKWEVGMIHFD a g t g g a c c a t g g c a g a a a t c g g t a c t g g c t t t c c a t t c g a c c c c c a t t a t g t g g a a g t c c t c a c c t g g t a c c g t c t t t a g c c a t g a c c g a a a g g t a a g c t g g g g g t a a t a c a c c t t c a g g

AleI 2940 20 25 30 35 LGERMHYVDVGPRDGTPVLF HaloTag® LGERMHYVDVGPRDGTPVLF QALAHVVDINTRAIAGRHQE t g g g c g a g c g c a t g c a c t a c g t c g a t g t t g g t c c g c g c g a t g g c a c c c c t g t g c t g t t c c a c c c g c t c g c g t a c g t g a t g c a g c t a c a a c c a g g c g c g c t a c c g t g g g g a c a c g a c a a g g

BstEII 3000 40 45 50 55 LHGNPTSSYVWRNIIPHVAP HaloTag® LHGNPTSSYVWRNIIPHVAP QVTVRGGGVHPAVDDRMNCR t g c a c g g t a a c c c g a c c t c c t c c t a c g t g t g g c g c a a c a t c a t c c c g c a t g t t g c a c c g a a c g t g c c a t t g g g c t g g a g g a g g a t g c a c a c c g c g t t g t a g t a g g g c g t a c a a c g t g g c t

3060 60 65 70 75 THRCIAPDLIGMGKSDKPDL HaloTag® THRCIAPDLIGMGKSDKPDL GMAANSWVQDTHAFGVFWVQ c c c a t c g c t g c a t t g c t c c a g a c c t g a t c g g t a t g g g c a a a t c c g a c a a a c c a g a c c t g g g g g t a g c g a c g t a a c g a g g t c t g g a c t a g c c a t a c c c g t t t a g g c t g t t t g g t c t g g a c c

BmgBI 3120 80 85 90 95 GYFFDDHVRFMDAFIEALGL HaloTag® GYFFDDHVRFMDAFIEALGL TIEEVVVDAEHIGEDFGQTQ g t t a t t t c t t c g a c g a c c a c g t c c g c t t c a t g g a t g c c t t c a t c g a a g c c c t g g g t c t g g c a a t a a a g a a g c t g c t g g t g c a g g c g a a g t a c c t a c g g a a g t a g c t t c g g g a c c c a g a c c

3180 100 105 110 115 EEVVLVIHDWGSALGFHWAK HaloTag® EEVVLVIHDWGSALGFHWAK FLDDQDNVVPAGSQTEVPGL a a g a g g t c g t c c t g g t c a t t c a c g a c t g g g g c t c c g c t c t g g g t t t c c a c t g g g c c a a g c t t c t c c a g c a g g a c c a g t a a g t g c t g a c c c c g a g g c g a g a c c c a a a g g t g a c c c g g t t c g

3240 120 125 130 135 RNPERVKGIAFMEFIRPIPT HaloTag® RNPERVKGIAFMEFIRPIPT AIWLADFTNCKHLEDARDRG g c a a t c c a g a g c g c g t c a a a g g t a t t g c a t t t a t g g a g t t c a t c c g c c c t a t c c c g a c c t c g t t a g g t c t c g c g c a g t t t c c a t a a c g t a a a t a c c t c a a g t a g g c g g g a t a g g g c t g g a

3300 140 145 150 155 WDEWPEFARETFQAFRTTDV HaloTag® WDEWPEFARETFQAFRTTDV PVFPWFKGALGELGEAGGVD g g g a c g a a t g g c c a g a a t t t g c c c g c g a g a c c t t c c a g g c c t t c c g c a c c a c c g a c g t c g c c c t g c t t a c c g g t c t t a a a c g g g c g c t c t g g a a g g t c c g g a a g g c g t g g t g g c t g c a g c

BspDI*ClaI* 3360 160 165 170 175 GRKLIIDQNVFIEGTLPMGV HaloTag® GRKLIIDQNVFIEGTLPMGV AALQDDILVNKDLTRQRHTD g c c g c a a g c t g a t c a t c g a t c a g a a c g t t t t t a t c g a g g g t a c g c t g c c g a t g g g t g t c g c g g c g t t c g a c t a g t a g c t a g t c t t g c a a a a a t a g c t c c c a t g c g a c g g c t a c c c a c a g c

PshAI 3420 180 185 190 195 VRPLTEVEMDHYREPFLNPV HaloTag® VRPLTEVEMDHYREPFLNPV DARQSFDLHVM t c c g c c c g c t g a c t g a a g t c g a g a t g g a c c a t t a c c g c g a g c c g t t c c t g a a t c c t g t t g a g g c g g g c g a c t g a c t t c a g c t c t a c c t g g t a a t g g c g c t c g g c a a g g a c t t a g g a c a a c

SgrAI 3480 200 205 210 215 DREPLWRFPNELPIAGEPAN HaloTag® DREPLWRFPNELPIAGEPAN a c c g c g a g c c a c t g t g g c g c t t c c c a a a c g a g c t g c c a a t c g c c g g t g a g c c a g c g a a c a t g g c g c t c g g t g a c a c c g c g a a g g g t t t g c t c g a c g g t t a g c g g c c a c t c g g t c g c t t g t

3540 220 225 230 235 IVALVEEYMDWLHQSPVPKL HaloTag® IVALVEEYMDWLHQSPVPKL t c g t c g c g c t g g t c g a a g a a t a c a t g g a c t g g c t g c a c c a g t c c c c t g t c c c g a a g c t g c a g c a g c g c g a c c a g c t t c t t a t g t a c c t g a c c g a c g t g g t c a g g g g a c a g g g c t t c g a c g

3600 240 245 250 255 LFWGTPGVLIPPAEAARLAK HaloTag® LFWGTPGVLIPPAEAARLAK t g t t c t g g g g c a c c c c a g g c g t t c t g a t c c c a c c g g c c g a a g c c g c t c g c c t g g c c a a a a a c a a g a c c c c g t g g g g t c c g c a a g a c t a g g g t g g c c g g c t t c g g c g a g c g g a c c g g t t t t

3660 260 265 270 275 SLPNCKAVDIGPGLNLLQED HaloTag® SLPNCKAVDIGPGLNLLQED g c c t g c c t a a c t g c a a g g c t g t g g a c a t c g g c c c g g g t c t g a a t c t g c t g c a a g a a g a c a c g g a c g g a t t g a c g t t c c g a c a c c t g t a g c c g g g c c c a g a c t t a g a c g a c g t t c t t c t g t

3720 280 285 290 295 NPDLIGSEIARWLSTLEISG HaloTag® NPDLIGSEIARWLSTLEISG a c c c g g a c c t g a t c g g c a g c g a g a t c g c g c g c t g g c t g t c g a c g c t c g a g a t t t c c g g c g t g g g c c t g g a c t a g c c g t c g c t c t a g c g c g c g a c c g a c a g c t g c g a g c t c t a a a g g c c g c

AsiSI 3780 EPTT 1 5 EDLYFQS (in frame with TEV site) DNAIATTLY TEV site attB1 EPTTEDLYFQSDNAIATTLY a g c c a a c c a c t g a g g a t c t g t a c t t t c a g a g c g a t a a c g c g a t c g c c a c a a c t t t g t a c a t c g g t t g g t g a c t c c t a g a c a t g a a a g t c t c g c t a t t g c g c t a g c g g t g t t g a a a c a t g t

3840 (in frame with TEV site) KKVGTMTEYKLVVVGAGGVG attB1 KKVGTMTEYKLVVVGAGGVG a a a a a g t t g g c a c c a t g a c t g a a t a t a a a c t t g t g g t a g t t g g a g c t g g t g g c g t a g g c a t t t t t c a a c c g t g g t a c t g a c t t a t a t t t g a a c a c c a t c a a c c t c g a c c a c c g c a t c c g t

3900 (in frame with TEV site) KSALTIQLIQNHFVDEYDPT KSALTIQLIQNHFVDEYDPT a g a g t g c c t t g a c g a t a c a g c t a a t t c a g a a t c a t t t t g t g g a c g a a t a t g a t c c a a c a a t c t c a c g g a a c t g c t a t g t c g a t t a a g t c t t a g t a a a a c a c c t g c t t a t a c t a g g t t g t t

3960 (in frame with TEV site) IEDSYRKQVVIDGETCLLDI IEDSYRKQVVIDGETCLLDI t a g a g g a t t c c t a c a g g a a g c a a g t a g t a a t t g a t g g a g a a a c c t g t c t c t t g g a t a t t c a t c t c c t a a g g a t g t c c t t c g t t c a t c a t t a a c t a c c t c t t t g g a c a g a g a a c c t a t a a g

4020 (in frame with TEV site) LDTAGQEEYSAMRDQYMRTG LDTAGQEEYSAMRDQYMRTG t c g a c a c a g c a g g t c a a g a g g a g t a c a g t g c a a t g a g g g a c c a g t a c a t g a g g a c t g g g g a g c t g t g t c g t c c a g t t c t c c t c a t g t c a c g t t a c t c c c t g g t c a t g t a c t c c t g a c c c c

4080 (in frame with TEV site) EGFLCVFAINNTKSFEDIHH EGFLCVFAINNTKSFEDIHH a g g g c t t t c t t t g t g t a t t t g c c a t a a a t a a t a c t a a a t c a t t t g a a g a t a t t c a c c a t t t c c c g a a a g a a a c a c a t a a a c g g t a t t t a t t a t g a t t t a g t a a a c t t c t a t a a g t g g t a a

4140 (in frame with TEV site) YREQIKRVKDSEDVPMVLVG YREQIKRVKDSEDVPMVLVG a t a g a g a a c a a a t t a a a a g a g t t a a g g a c t c t g a a g a t g t a c c t a t g g t c c t a g t a g g a a t a t c t c t t g t t t a a t t t t c t c a a t t c c t g a g a c t t c t a c a t g g a t a c c a g g a t c a t c c t t

4200 (in frame with TEV site) NKCDLPSRTVDTKQAQDLAR NKCDLPSRTVDTKQAQDLAR a t a a a t g t g a t t t g c c t t c c a g a a c a g t a g a c a c a a a a c a g g c t c a g g a c t t a g c a a g a a t a t t t a c a c t a a a c g g a a g g t c t t g t c a t c t g t g t t t t g t c c g a g t c c t g a a t c g t t c t t

EcoRI 4260 (in frame with TEV site) SYGIPFIETSAKTRQRVEDA SYGIPFIETSAKTRQRVEDA g t t a t g g a a t t c c t t t t a t t g a a a c a t c a g c a a a g a c a a g a c a g a g a g t g g a g g a t g c t t c a a t a c c t t a a g g a a a a t a a c t t t g t a g t c g t t t c t g t t c t g t c t c t c a c c t c c t a c g a a

4320 (in frame with TEV site) FYTLVREIRQYRLKKISKEE FYTLVREIRQYRLKKISKEE t t t a t a c a t t g g t g a g a g a g a t c c g a c a a t a c a g a t t g a a a a a a a t c a g c a a a g a a g a a a a a a t a t g t a a c c a c t c t c t c t a g g c t g t t a t g t c t a a c t t t t t t t a g t c g t t t c t t c t t t

4380 (in frame with TEV site) KTPGCVKIKKCIIM* attB2 KTPGCVKIKKCIIM* a g a c t c c t g g c t g t g t g a a a a t t a a a a a a t g c a t t a t a a t g t a a t c a a c t t t c t t g t a c a t c t g a g g a c c g a c a c a c t t t t a a t t t t t t a c g t a a t a t t a c a t t a g t t g a a a g a a c a t g t

XbaIPspXINotI 4440 attB2 a a g t t g t g a a a t t c t g c a g a t a t c c a t c a c a c t g g c g g c c g c t c g a g c a t g c a t c t a g a g t t c a a c a c t t t a a g a c g t c t a t a g g t a g t g t g a c c g c c g g c g a g c t c g t a c g t a g a t c t c

4500 SP6 promoter bGH poly(A) signal G A T A T C A C A G T G G A T T T A SP6 G G A G C T G A C A C G G A BGH-rev g g c c c t a t t c t a t a g t g t c a c c t a a a t g c t a g a g c t c g c t g a t c a g c c t c g a c t g t g c c t c c g g g a t a a g a t a t c a c a g t g g a t t t a c g a t c t c g a g c g a c t a g t c g g a g c t g a c a c g g a

4560 bGH poly(A) signal A G A T BGH-rev t c t a g t t g c c a g c c a t c t g t t g t t t g c c c c t c c c c c g t g c c t t c c t t g a c c c t g g a a g g t a g a t c a a c g g t c g g t a g a c a a c a a a c g g g g a g g g g g c a c g g a a g g a a c t g g g a c c t t c c a

4620 bGH poly(A) signal g c c a c t c c c a c t g t c c t t t c c t a a t a a a a t g a g g a a a t t g c a t c g c a t t g t c t g a g t a g g c g g t g a g g g t g a c a g g a a a g g a t t a t t t t a c t c c t t t a a c g t a g c g t a a c a g a c t c a t c c

4680 bGH poly(A) signal t g t c a t t c t a t t c t g g g g g g t g g g g t g g g g c a g g a c a g c a a g g g g g a g g a t t g g g a a g a c a c a g t a a g a t a a g a c c c c c c a c c c c a c c c c g t c c t g t c g t t c c c c c t c c t a a c c c t t c t g

4740 bGH poly(A) signal a a t a g c a g g c a t g c t g g g g a t g c g g t g g g c t c t a t g g c t t c t g a g g c g g a a a g a a c c a g c t t a t c g t c c g t a c g a c c c c t a c g c c a c c c g a g a t a c c g a a g a c t c c g c c t t t c t t g g t c g

4800 f1 ori t g g g g c t c t a g g g g g t a t c c c c a c g c g c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g a c c c c g a g a t c c c c c a t a g g g g t g c g c g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c

4860 f1 ori G A G G A A A G C G A F1ori-R g t g g t t a c g c g c a g c g t g a c c g c t a c a c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t c a c c a a t g c g c g t c g c a c t g g c g a t g t g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a

4920 f1 ori A A G A A G G G A F1ori-R t t c t t c c c t t c c t t t c t c g c c a c g t t c g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g a a g a a g g g a a g g a a a g a g c g g t g c a a g c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c

4980 f1 ori c t c c c t t t a g g g t t c c g a t t t a g t g c t t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g a g g g a a a t c c c a a g g c t a a a t c a c g a a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c

DraIII 5040 f1 ori g g t g a t g g t t c a c g t a g t g g g c c a t c g c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g c c a c t a c c a a g t g c a t c a c c c g g t a g c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c

5100 f1 ori G T G G A C T C T T G T T C C A A A C T G G F1ori-F g a g t c c a c g t t c t t t a a t a g t g g a c t c t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c c t c a g g t g c a a g a a a t t a t c a c c t g a g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g

5160 f1 ori t c g g t c t a t t c t t t t g a t t t a t a a g g g a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t a g c c a g a t a a g a a a a c t a a a t a t t c c c t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a

5220 f1 ori SV40 promoter C C T T A C A C A C A G T C A A T C C C A pBABE 3' g a g c t g a t t t a a c a a a a a t t t a a c g c g a a t t a a t t c t g t g g a a t g t g t g t c a g t t a g g g t c t c g a c t a a a t t g t t t t t a a a t t g c g c t t a a t t a a g a c a c c t t a c a c a c a g t c a a t c c c a

5280 SV40 promoter g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a

SexAI*CsiI 5340 SV40 promoter c a g c a a c c a g g t g t g g a a a g t c c c t a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g c a t g c g t c g t t g g t c c a c a c c t t t c a g g g a t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c g t a c g

5400 SV40 promoter SV40 ori a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c c a t c c c g c c c c t a a c t c t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g g t a g g g c g g g g a t t g a g

5460 SV40 promoter SV40 ori T A T T T A T G C A G A G G SV40pro-F c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t t t t t a t t t a t g c a g a g g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a a a a a t a a a t a c g t c t c c

5520 SV40 promoter SV40 ori C C G A G G SV40pro-F c c g a g g c c g c c t c t g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a g g c t t t t t t g g a g g c c g g c t c c g g c g g a g a c g g a g a c t c g a t a a g g t c t t c a t c a c t c c t c c g a a a a a a c c t c c g g

5580 SV40 promoter t a g g c t t t t g c a a a a a g c t c c c g g g a g c t t g t a t a t c c a t t t t c g g a t c t g a t c a a g a g a a t c c g a a a a c g t t t t t c g a g g g c c c t c g a a c a t a t a g g t a a a a g c c t a g a c t a g t t c t c t

5640 1 5 10 MIEQDGLHAGSPA NeoR/KanR MIEQDGLHAGSPA c a g g a t g a g g a t c g t t t c g c a t g a t t g a a c a a g a t g g a t t g c a c g c a g g t t c t c c g g c c g g t c c t a c t c c t a g c a a a g c g t a c t a a c t t g t t c t a c c t a a c g t g c g t c c a a g a g g c c g g c

5700 15 20 25 30 AWVERLFGYDWAQQTIGCSD NeoR/KanR G A T A A G C C G A T A C T G A C C C G Neo-R AWVERLFGYDWAQQTIGCSD c t t g g g t g g a g a g g c t a t t c g g c t a t g a c t g g g c a c a a c a g a c a a t c g g c t g c t c t g a t g g a a c c c a c c t c t c c g a t a a g c c g a t a c t g a c c c g t g t t g t c t g t t a g c c g a c g a g a c t a c

KasINarISfoIPluTI 5760 35 40 45 50 AAVFRLSAQGRPVLFVKTDL NeoR/KanR AAVFRLSAQGRPVLFVKTDL c c g c c g t g t t c c g g c t g t c a g c g c a g g g g c g c c c g g t t c t t t t t g t c a a g a c c g a c c t g t g g c g g c a c a a g g c c g a c a g t c g c g t c c c c g c g g g c c a a g a a a a a c a g t t c t g g c t g g a c a

5820 55 60 65 70 SGALNELQDEAARLSWLATT NeoR/KanR SGALNELQDEAARLSWLATT c c g g t g c c c t g a a t g a a c t g c a g g a c g a g g c a g c g c g g c t a t c g t g g c t g g c c a c g a c g g g g c c a c g g g a c t t a c t t g a c g t c c t g c t c c g t c g c g c c g a t a g c a c c g a c c g g t g c t g c c

5880 75 80 85 90 GVPCAAVLDVVTEAGRDWLL NeoR/KanR GVPCAAVLDVVTEAGRDWLL g c g t t c c t t g c g c a g c t g t g c t c g a c g t t g t c a c t g a a g c g g g a a g g g a c t g g c t g c t a t c g c a a g g a a c g c g t c g a c a c g a g c t g c a a c a g t g a c t t c g c c c t t c c c t g a c c g a c g a t a

5940 95 100 105 110 LGEVPGQDLLSSHLAPAEKV NeoR/KanR LGEVPGQDLLSSHLAPAEKV *RVKSRGLFY t g g g c g a a g t g c c g g g g c a g g a t c t c c t g t c a t c t c a c c t t g c t c c t g c c g a g a a a g t a t a c c c g c t t c a c g g c c c c g t c c t a g a g g a c a g t a g a g t g g a a c g a g g a c g g c t c t t t c a t a

6000 115 120 125 130 SIMADAMRRLHTLDPATCPF NeoR/KanR SIMADAMRRLHTLDPATCPF GDHSICHPPQMRKIRSGAWE c c a t c a t g g c t g a t g c a a t g c g g c g g c t g c a t a c g c t t g a t c c g g c t a c c t g c c c a t t c g g g t a g t a c c g a c t a c g t t a c g c c g c c g a c g t a t g c g a a c t a g g c c g a t g g a c g g g t a a g c

6060 135 140 145 150 DHQAKHRIERARTRMEAGLV NeoR/KanR DHQAKHRIERARTRMEAGLV VVLRFMADLSCTSPHFGTKD a c c a c c a a g c g a a a c a t c g c a t c g a g c g a g c a c g t a c t c g g a t g g a a g c c g g t c t t g t c g t g g t g g t t c g c t t t g t a g c g t a g c t c g c t c g t g c a t g a g c c t a c c t t c g g c c a g a a c a g c

6120 155 160 165 170 DQDDLDEEHQGLAPAELFAR NeoR/KanR DQDDLDEEHQGLAPAELFAR ILIIQVFLMLPERWGFQEGP a t c a g g a t g a t c t g g a c g a a g a g c a t c a g g g g c t c g c g c c a g c c g a a c t g t t c g c c a g g c t a g t c c t a c t a g a c c t g c t t c t c g t a g t c c c c g a g c g c g g t c g g c t t g a c a a g c g g t c c g

6180 175 180 185 190 LKARMPDGEDLVVTHGDACL NeoR/KanR LKARMPDGEDLVVTHGDACL ELRAHGVALIEDHGMAIGAQ t c a a g g c g c g c a t g c c c g a c g g c g a g g a t c t c g t c g t g a c c c a t g g c g a t g c c t g c t t g c a g t t c c g c g c g t a c g g g c t g c c g c t c c t a g a g c a g c a c t g g g t a c c g c t a c g g a c g a a c g

6240 195 200 205 210 PNIMVENGRFSGFIDCGRLG NeoR/KanR PNIMVENGRFSGFIDCGRLG RIDHHFIAAKRSEDVTAPQT c g a a t a t c a t g g t g g a a a a t g g c c g c t t t t c t g g a t t c a t c g a c t g t g g c c g g c t g g g t g g c t t a t a g t a c c a c c t t t t a c c g g c g a a a a g a c c t a a g t a g c t g a c a c c g g c c g a c c c a c

RsrII 6300 215 220 225 230 VADRYQDIALATRDIAEELG NeoR/KanR C G T T G G C T A C C C G T G A T A T T Neo-F VADRYQDIALATRDIAEELG HRVAILVYRQSGTINSFLKA t g g c g g a c c g c t a t c a g g a c a t a g c g t t g g c t a c c c g t g a t a t t g c t g a a g a g c t t g g c g a c c g c c t g g c g a t a g t c c t g t a t c g c a a c c g a t g g g c a c t a t a a c g a c t t c t c g a a c c g c

6360 235 240 245 250 GEWADRFLVLYGIAAPDSQR NeoR/KanR GEWADRFLVLYGIAAPDSQR AFPSVAEEHKVTDGSGIRLA g c g a a t g g g c t g a c c g c t t c c t c g t g c t t t a c g g t a t c g c c g c t c c c g a t t c g c a g c g c a c g c t t a c c c g a c t g g c g a a g g a g c a c g a a a t g c c a t a g c g g c g a g g g c t a a g c g t c g c g t

BstBI 6420 255 260 265 IAFYRLLDEFF* NeoR/KanR IAFYRLLDEFF* DGEIAKKVLEESRSEPTRFS t c g c c t t c t a t c g c c t t c t t g a c g a g t t c t t c t g a g c g g g a c t c t g g g g t t c g a a a t g a c a g c g g a a g a t a g c g g a a g a a c t g c t c a a g a a g a c t c g c c c t g a g a c c c c a a g c t t t a c t g

6480 RGLSAWGAM c g a c c a a g c g a c g c c c a a c c t g c c a t c a c g a g a t t t c g a t t c c a c c g c c g c c t t c t a t g a g c t g g t t c g c t g c g g g t t g g a c g g t a g t g c t c t a a a g c t a a g g t g g c g g c g g a a g a t a c t

PfoI 6540 a a g g t t g g g c t t c g g a a t c g t t t t c c g g g a c g c c g g c t g g a t g a t c c t c c a g c g c g g g g a t t c c a a c c c g a a g c c t t a g c a a a a g g c c c t g c g g c c g a c c t a c t a g g a g g t c g c g c c c c t

6600 SV40 poly(A) signal t c t c a t g c t g g a g t t c t t c g c c c a c c c c a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a g a g t a c g a c c t c a a g a a g c g g g t g g g g t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t

BsmI 6660 SV40 poly(A) signal G EBV-rev C G T T A T C G T A G T G T T T A A A G SV40pA-R a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c

6720 SV40 poly(A) signal T G G T T T G T C C A A A C T C A T C EBV-rev t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g t a t a c c g t c g a c c t c t a g c t a a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c a t a t g g c a g c t g g a g a t c g a t

6780 M13 rev lac operator C A G T A T C G A C A A A G G A C M13 Reverse G G A C A C A C T T T A A C A A T A G G C G A M13/pUC Reverse g a g c t t g g c g t a a t c a t g g t c a t a g c t g t t t c c t g t g t g a a a t t g t t a t c c g c t c a c a a t c t c g a a c c g c a t t a g t a c c a g t a t c g a c a a a g g a c a c a c t t t a a c a a t a g g c g a g t g t t a

6840 -10 -35 lac promoter CAP binding site t c c a c a c a a c a t a c g a g c c g g a a g c a t a a a g t g t a a a g c c t g g g g t g c c t a a t g a g t g a g a g g t g t g t t g t a t g c t c g g c c t t c g t a t t t c a c a t t t c g g a c c c c a c g g a t t a c t c a c t c

6900 CAP binding site c t a a c t c a c a t t a a t t g c g t t g c g c t c a c t g c c c g c t t t c c a g t c g g g a a a c c t g t c g t g g a t t g a g t g t a a t t a a c g c a a c g c g a g t g a c g g g c g a a a g g t c a g c c c t t t g g a c a g c a c

6960 c c a g c t g c a t t a a t g a a t c g g c c a a c g c g c g g g g a g a g g c g g t t t g c g t a t t g g g c g c t c g g t c g a c g t a a t t a c t t a g c c g g t t g c g c g c c c c t c t c c g c c a a a c g c a t a a c c c g c g a g

7020 G A G C G A G T G A C T G A G C G A L4440 t t c c g c t t c c t c g c t c a c t g a c t c g c t g c g c t c g g t c g t t c g g c t g c g g c g a g c g g t a t c a a g g c g a a g g a g c g a g t g a c t g a g c g a c g c g a g c c a g c a a g c c g a c g c c g c t c g c c a t a g

7080 a g c t c a c t c a a a g g c g g t a a t a c g g t t a t c c a c a g a a t c a g g g g a t a a c g c a g g a a a g a a t c g a g t g a g t t t c c g c c a t t a t g c c a a t a g g t g t c t t a g t c c c c t a t t g c g t c c t t t c t t

7140 c a t g t g a g c a a a a g g c c a g c a a a a g g c c a g g a a c c g t a a a a a g g c c g c g t t g c t g g c g t t g t a c a c t c g t t t t c c g g t c g t t t t c c g g t c c t t g g c a t t t t t c c g g c g c a a c g a c c g c a a

7200 ori t t t c c a t a g g c t c c g c c c c c c t g a c g a g c a t c a c a a a a a t c g a c g c t c a a g t c a g a g g t g a a a g g t a t c c g a g g c g g g g g g a c t g c t c g t a g t g t t t t t a g c t g c g a g t t c a g t c t c c a c

7260 ori T T T C T A T G G T C C G C A A A G G G pBR322ori-F g c g a a a c c c g a c a g g a c t a t a a a g a t a c c a g g c g t t t c c c c c t g g a a g c t c c c t c g t g c g c g c t t t g g g c t g t c c t g a t a t t t c t a t g g t c c g c a a a g g g g g a c c t t c g a g g g a g c a c g c

7320 ori c t c t c c t g t t c c g a c c c t g c c g c t t a c c g g a t a c c t g t c c g c c t t t c t c c c t t c g g g a a g g a g a g g a c a a g g c t g g g a c g g c g a a t g g c c t a t g g a c a g g c g g a a a g a g g g a a g c c c t t c

7380 ori c g t g g c g c t t t c t c a t a g c t c a c g c t g t a g g t a t c t c a g t t c g g t g t a g g t c g t t c g c t c g c a c c g c g a a a g a g t a t c g a g t g c g a c a t c c a t a g a g t c a a g c c a c a t c c a g c a a g c g a g

7440 ori c a a g c t g g g c t g t g t g c a c g a a c c c c c c g t t c a g c c c g a c c g c t g c g c c t t a t c c g g t a a g t t c g a c c c g a c a c a c g t g c t t g g g g g g c a a g t c g g g c t g g c g a c g c g g a a t a g g c c a t t

7500 ori c t a t c g t c t t g a g t c c a a c c c g g t a a g a c a c g a c t t a t c g c c a c t g g c a g c a g c c a c t g g g a t a g c a g a a c t c a g g t t g g g c c a t t c t g t g c t g a a t a g c g g t g a c c g t c g t c g g t g a c c

7560 ori t a a c a g g a t t a g c a g a g c g a g g t a t g t a g g c g g t g c t a c a g a g t t c t t g a a g t g g t g g c c a t t g t c c t a a t c g t c t c g c t c c a t a c a t c c g c c a c g a t g t c t c a a g a a c t t c a c c a c c g g

7620 ori t a a c t a c g g c t a c a c t a g a a g a a c a g t a t t t g g t a t c t g c g c t c t g c t g a a g c c a g t t a c a t t g a t g c c g a t g t g a t c t t c t t g t c a t a a a c c a t a g a c g c g a g a c g a c t t c g g t c a a t g

7680 ori c t t c g g a a a a a g a g t t g g t a g c t c t t g a t c c g g c a a a c a a a c c a c c g c t g g t a g c g g t g g g a a g c c t t t t t c t c a a c c a t c g a g a a c t a g g c c g t t t g t t t g g t g g c g a c c a t c g c c a c c

7740 ori t t t t t t t g t t t g c a a g c a g c a g a t t a c g c g c a g a a a a a a a g g a t c t c a a g a a g a t c c t t t a a a a a a a c a a a c g t t c g t c g t c t a a t g c g c g t c t t t t t t t c c t a g a g t t c t t c t a g g a a a

7800 g a t c t t t t c t a c g g g g t c t g a c g c t c a g t g g a a c g a a a a c t c a c g t t a a g g g a t t t t g g t c t a g a a a a g a t g c c c c a g a c t g c g a g t c a c c t t g c t t t t g a g t g c a a t t c c c t a a a a c c a

7860 c a t g a g a t t a t c a a a a a g g a t c t t c a c c t a g a t c c t t t t a a a t t a a a a a t g a a g t t t t a a g t a c t c t a a t a g t t t t t c c t a g a a g t g g a t c t a g g a a a a t t t a a t t t t t a c t t c a a a a t t

7920 285 *WHKILS AmpR *WHKILS a t c a a t c t a a a g t a t a t a t g a g t a a a c t t g g t c t g a c a g t t a c c a a t g c t t a a t c a g t g a t a g t t a g a t t t c a t a t a t a c t c a t t t g a a c c a g a c t g t c a a t g g t t a c g a a t t a g t c a c t

7980 280 275 270 265 AGIEAIQRNREDMTAQSGTT AmpR AGIEAIQRNREDMTAQSGTT g g c a c c t a t c t c a g c g a t c t g t c t a t t t c g t t c a t c c a t a g t t g c c t g a c t c c c c g t c g t c c g t g g a t a g a g t c g c t a g a c a g a t a a a g c a a g t a g g t a t c a a c g g a c t g a g g g g c a g c a

8040 260 255 250 245 YIVVIRSPKGDPGLAAIIGR AmpR YIVVIRSPKGDPGLAAIIGR g t a g a t a a c t a c g a t a c g g g a g g g c t t a c c a t c t g g c c c c a g t g c t g c a a t g a t a c c g c g c a t c t a t t g a t g c t a t g c c c t c c c g a a t g g t a g a c c g g g g t c a c g a c g t t a c t a t g g c g c

8100 240 235 230 225 SGREGAGSKDAIFWGAPLAS AmpR SGREGAGSKDAIFWGAPLAS a g a c c c a c g c t c a c c g g c t c c a g a t t t a t c a g c a a t a a a c c a g c c a g c c g g a a g g g c c g a t c t g g g t g c g a g t g g c c g a g g t c t a a a t a g t c g t t a t t t g g t c g g t c g g c c t t c c c g g c t

8160 220 215 210 205 RLLPGAVKDAEMWDILQQRS AmpR RLLPGAVKDAEMWDILQQRS g c g c a g a a g t g g t c c t g c a a c t t t a t c c g c c t c c a t c c a g t c t a t t a a t t g t t g c c g g g a c g c g t c t t c a c c a g g a c g t t g a a a t a g g c g g a g g t a g g t c a g a t a a t t a a c a a c g g c c c t

8220 200 195 190 185 ALTLLEGTLLKRLTTAMAVP AmpR ALTLLEGTLLKRLTTAMAVP a g c t a g a g t a a g t a g t t c g c c a g t t a a t a g t t t g c g c a a c g t t g t t g c c a t t g c t a c a g g t c g a t c t c a t t c a t c a a g c g g t c a a t t a t c a a a c g c g t t g c a a c a a c g g t a a c g a t g t c c

8280 180 175 170 165 MTTDREDNPIAENLEPEWRD AmpR MTTDREDNPIAENLEPEWRD c a t c g t g g t g t c a c g c t c g t c g t t t g g t a t g g c t t c a t t c a g c t c c g g t t c c c a a c g a t c g t a g c a c c a c a g t g c g a g c a g c a a a c c a t a c c g a a g t a a g t c g a g g c c a a g g g t t g c t a g

8340 160 155 150 145 LRTVHDGMNHLFATLEKPGG AmpR LRTVHDGMNHLFATLEKPGG a a g g c g a g t t a c a t g a t c c c c c a t g t t g t g c a a a a a a g c g g t t a g c t c c t t c g g t c c t c c t t c c g c t c a a t g t a c t a g g g g g t a c a a c a c g t t t t t t c g c c a a t c g a g g a a g c c a g g a g g

8400 140 135 130 125 ITTLLLNAATNDSMTIAASC AmpR ITTLLLNAATNDSMTIAASC g a t c g t t g t c a g a a g t a a g t t g g c c g c a g t g t t a t c a c t c a t g g t t a t g g c a g c a c t g c a c t a g c a a c a g t c t t c a t t c a a c c g g c g t c a c a a t a g t g a g t a c c a a t a c c g t c g t g a c g t

ScaI 8460 120 115 110 105 LERVTMGDTLHKETVPSYEV AmpR LERVTMGDTLHKETVPSYEV t a a t t c t c t t a c t g t c a t g c c a t c c g t a a g a t g c t t t t c t g t g a c t g g t g a g t a c t c a a c a t t a a g a g a a t g a c a g t a c g g t a g g c a t t c t a c g a a a a g a c a c t g a c c a c t c a t g a g t t g

8520 100 95 90 85 LDNQSYHIRRGLQEQGADIR AmpR LDNQSYHIRRGLQEQGADIR c a a g t c a t t c t g a g a a t a g t g t a t g c g g c g a c c g a g t t g c t c t t g c c c g g c g t c a a t a c g g t t c a g t a a g a c t c t t a t c a c a t a c g c c g c t g g c t c a a c g a g a a c g g g c c g c a g t t a t g c

8580 80 75 70 65 SLVAGCLLVKFTSMMPFREE AmpR A T A A T A C C G C G C C A C A T A G C Amp-R SLVAGCLLVKFTSMMPFREE g g a t a a t a c c g c g c c a c a t a g c a g a a c t t t a a a a g t g c t c a t c a t t g g a a a a c g t t c t t c c c t a t t a t g g c g c g g t g t a t c g t c t t g a a a t t t t c a c g a g t a g t a a c c t t t t g c a a g a a g

8640 60 55 50 45 PRFSELIKGSNLDLEIYGVR AmpR PRFSELIKGSNLDLEIYGVR g g g g c g a a a a c t c t c a a g g a t c t t a c c g c t g t t g a g a t c c a g t t c g a t g t a a c c c a c t c g c c c c g c t t t t g a g a g t t c c t a g a a t g g c g a c a a c t c t a g g t c a a g c t a c a t t g g g t g a g c

8700 40 35 30 25 AGLQDEADKVKVLTEPH signal sequence AFV AmpR AGLQDEADKVKVLTEPHAFV t g c a c c c a a c t g a t c t t c a g c a t c t t t t a c t t t c a c c a g c g t t t c t g g g t g a g c a a a a a c a c g t g g g t t g a c t a g a a g t c g t a g a a a a t g a a a g t g g t c g c a a a g a c c c a c t c g t t t t t g

8760 20 15 10 5 1 signal sequence PLCFAAFFPILAVRFHQISM AmpR PLCFAAFFPILAVRFHQISM a g g a a g g c a a a a t g c c g c a a a a a a g g g a a t a a g g g c g a c a c g g a a a t g t t g a a t a c t c a t t c c t t c c g t t t t a c g g c g t t t t t t c c c t t a t t c c c g c t g t g c c t t t a c a a c t t a t g a g t a

SspI 8820 AmpR promoter a c t c t t c c t t t t t c a a t a t t a t t g a a g c a t t t a t c a g g g t t a t t g t c t c a t g a g c g g a t a t g a g a a g g a a a a a g t t a t a a t a a c t t c g t a a a t a g t c c c a a t a a c a g a g t a c t c g c c t a t

8880 AmpR promoter c a t a t t t g a a t g t a t t t a g a a a a a t a a a c a a a t a g g g g t t c c g c g c a c a t t t c c c c g a a a g t a t a a a c t t a c a t a a a t c t t t t t a t t t g t t t a t c c c c a a g g c g c g t g t a a a g g g g c t t t

SgrDI 3ʹ 5ʹ 8896 a g t g c c a c c t g a c g t c t c a c g g t g g a c t g c a g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.