Addgene pUltra-Plus-H2B-EGFP - Sequence Analyzer Skip to main content
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Sequence Analyzer: pUltra-Plus-H2B-EGFP

Map View

M13 rev M13 Reverse (8650 .. 8666) lac operator M13/pUC Reverse (8631 .. 8653) CAP binding site L4440 (8418 .. 8435) AflIII - PciI (8320) pBR322ori-F (8165 .. 8184) AhdI (7432) ScaI (6951) Amp-R (6863 .. 6882) F1ori-F (6353 .. 6374) F1ori-R (6143 .. 6162) M13/pUC Forward (5904 .. 5926) M13 Forward (5895 .. 5912) M13 fwd T7 (5866 .. 5885) T7 promoter SfiI (5798) EBV-rev (5641 .. 5660) SV40pA-R (5587 .. 5606) SnaBI (5256) KpnI (5178) Acc65I (5174) PflMI (4807) WPRE-R (4619 .. 4639) HincII (4557) SalI (4555) BspEI (4546) BclI * (4532) BmtI (4527) NheI (4523) T3 (8685 .. 8705) SphI (98) HIV-1 Ψ NruI * (609) NotI (920) MfeI (961) BbvCI (1196) PacI (1993) hUBCpro-F (3065 .. 3084) SpeI (3178) SgrAI (3254) EcoRV (3262) XbaI (3335) BstXI (3517) MscI (3662) BamHI (3718) EGFP-N (3782 .. 3803) EXFP-R (4043 .. 4062) EGFP-C (4390 .. 4411) pUltra-Plus-H2B-EGFP 8724 bp

Sequence View

5ʹ 3ʹ 60 a g c t t a a t g t a g t c t t a t g c a a t a c t c t t g t a g t c t t g c a a c a t g g t a a c g a t g a g t t a g t c g a a t t a c a t c a g a a t a c g t t a t g a g a a c a t c a g a a c g t t g t a c c a t t g c t a c t c a a t c

SphI 120 c a a c a t g c c t t a c a a g g a g a g a a a a a g c a c c g t g c a t g c c g a t t g g t g g a a g t a a g g t g g g t t g t a c g g a a t g t t c c t c t c t t t t t c g t g g c a c g t a c g g c t a a c c a c c t t c a t t c c a c c

180 t a c g a t c g t g c c t t a t t a g g a a g g c a a c a g a c g g g t c t g a c a t g g a t t g g a c g a a c c a c t a t g c t a g c a c g g a a t a a t c c t t c c g t t g t c t g c c c a g a c t g t a c c t a a c c t g c t t g g t g a

240 5' LTR (truncated) g a a t t g c c g c a t t g c a g a g a t a t t g t a t t t a a g t g c c t a g c t c g a t a c a a t a a a c g g g t c c t t a a c g g c g t a a c g t c t c t a t a a c a t a a a t t c a c g g a t c g a g c t a t g t t a t t t g c c c a g

300 5' LTR (truncated) t c t c t g g t t a g a c c a g a t c t g a g c c t g g c t c t c t g g c t a a c t a g g g a a c c c a c t g c t t a a a g a g a c c a a t c t g g t c t a g a c t c g g a c c g a g a g a c c g a t t g a t c c c t t g g g t g a c g a a t t

360 5' LTR (truncated) g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g t g t g t g c c c g t c t g t t g t g t g a c t c c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c a c a c a c g g g c a g a c a a c a c a c t g a g

420 5' LTR (truncated) t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g t g t g g a a a a t c t c t a g c a g t g g c g c a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c a c a c c t t t t a g a g a t c g t c a c c g c g

480 HIV-1 Ψ c c g a a c a g g g a c c t g a a a g c g a a a g g g a a a c c a g a g c t c t c t c g a c g c a g g a c t c g g c t t g g c t t g t c c c t g g a c t t t c g c t t t c c c t t t g g t c t c g a g a g a g c t g c g t c c t g a g c c g a a

540 HIV-1 Ψ g c t g a a g c g c g c a c g g c a a g a g g c g a g g g g c g g c g a c t g g t g a g t a c g c c a a a a a t t t t g c g a c t t c g c g c g t g c c g t t c t c c g c t c c c c g c c g c t g a c c a c t c a t g c g g t t t t t a a a a c

600 HIV-1 Ψ a c t a g c g g a g g c t a g a a g g a g a g a g a t g g g t g c g a g a g c g t c a g t a t t a a g c g g g g g a g a t g a t c g c c t c c g a t c t t c c t c t c t c t a c c c a c g c t c t c g c a g t c a t a a t t c g c c c c c t c t

NruI* 660 a t t a g a t c g c g a t g g g a a a a a a t t c g g t t a a g g c c a g g g g g a a a g a a a a a a t a t a a a t t a t a a t c t a g c g c t a c c c t t t t t t a a g c c a a t t c c g g t c c c c c t t t c t t t t t t a t a t t t a a t

720 a a a c a t a t a g t a t g g g c a a g c a g g g a g c t a g a a c g a t t c g c a g t t a a t c c t g g c c t g t t a t t t g t a t a t c a t a c c c g t t c g t c c c t c g a t c t t g c t a a g c g t c a a t t a g g a c c g g a c a a t

780 g a a a c a t c a g a a g g c t g t a g a c a a a t a c t g g g a c a g c t a c a a c c a t c c c t t c a g a c a g g a c t t t g t a g t c t t c c g a c a t c t g t t t a t g a c c c t g t c g a t g t t g g t a g g g a a g t c t g t c c t

840 t c a g a a g a a c t t a g a t c a t t a t a t a a t a c a g t a g c a a c c c t c t a t t g t g t g c a t c a a a g g a g t c t t c t t g a a t c t a g t a a t a t a t t a t g t c a t c g t t g g g a g a t a a c a c a c g t a g t t t c c

900 a t a g a g a t a a a a g a c a c c a a g g a a g c t t t a g a c a a g a t a g a g g a a g a g c a a a a c a a a a g t t a t c t c t a t t t t c t g t g g t t c c t t c g a a a t c t g t t c t a t c t c c t t c t c g t t t t g t t t t c a

NotI 960 MRD a a g a c c a c c g c a c a g c a a g c g g c c g c t g a t c t t c a g a c c t g g a g g a g g a g a t a t g a g g g a t t c t g g t g g c g t g t c g t t c g c c g g c g a c t a g a a g t c t g g a c c t c c t c c t c t a t a c t c c c t

MfeI 1020 NWRSELYKYKVVKIEPLGVA c a a t t g g a g a a g t g a a t t a t a t a a a t a t a a a g t a g t a a a a a t t g a a c c a t t a g g a g t a g c g t t a a c c t c t t c a c t t a a t a t a t t t a t a t t t c a t c a t t t t t a a c t t g g t a a t c c t c a t c g

1080 RRE PTKAKRRVVQREKRAVGIGA a c c c a c c a a g g c a a a g a g a a g a g t g g t g c a g a g a g a a a a a a g a g c a g t g g g a a t a g g a g c t g g g t g g t t c c g t t t c t c t t c t c a c c a c g t c t c t c t t t t t t c t c g t c a c c c t t a t c c t c g

1140 RRE LFLGFLGAAGSTMGAASMTL t t t g t t c c t t g g g t t c t t g g g a g c a g c a g g a a g c a c t a t g g g c g c a g c g t c a a t g a c g c t a a a c a a g g a a c c c a a g a a c c c t c g t c g t c c t t c g t g a t a c c c g c g t c g c a g t t a c t g c g a

BbvCI 1200 RRE TVQARQLLSGIVQQQNNLLR g a c g g t a c a g g c c a g a c a a t t a t t g t c t g g t a t a g t g c a g c a g c a g a a c a a t t t g c t g a g c t g c c a t g t c c g g t c t g t t a a t a a c a g a c c a t a t c a c g t c g t c g t c t t g t t a a a c g a c t c

1260 RRE AIEAQQHLLQLTVWGIKQLQ g g c t a t t g a g g c g c a a c a g c a t c t g t t g c a a c t c a c a g t c t g g g g c a t c a a g c a g c t c c a c c g a t a a c t c c g c g t t g t c g t a g a c a a c g t t g a g t g t c a g a c c c c g t a g t t c g t c g a g g t

1320 RRE ARILAVERYLKDQQLLGIWG g g c a a g a a t c c t g g c t g t g g a a a g a t a c c t a a a g g a t c a a c a g c t c c t g g g g a t t t g g g g c c g t t c t t a g g a c c g a c a c c t t t c t a t g g a t t t c c t a g t t g t c g a g g a c c c c t a a a c c c c

1380 CSGKLICTTAVPWNASWSNK t t g c t c t g g a a a a c t c a t t t g c a c c a c t g c t g t g c c t t g g a a t g c t a g t t g g a g t a a t a a a a c g a g a c c t t t t g a g t a a a c g t g g t g a c g a c a c g g a a c c t t a c g a t c a a c c t c a t t a t t

1440 SLEQIWNHTTWMEWDREINN a t c t c t g g a a c a g a t t t g g a a t c a c a c g a c c t g g a t g g a g t g g g a c a g a g a a a t t a a c a a t a g a g a c c t t g t c t a a a c c t t a g t g t g c t g g a c c t a c c t c a c c c t g t c t c t t t a a t t g t t

1500 1 KNE gp41 peptide YTSLIHSLIEESQNQQEKNE t t a c a c a a g c t t a a t a c a c t c c t t a a t t g a a g a a t c g c a a a a c c a g c a a g a a a a g a a t g a a a t g t g t t c g a a t t a t g t g a g g a a t t a a c t t c t t a g c g t t t t g g t c g t t c t t t t c t t a c t

1560 5 10 15 QELLELDKWASL (in frame with gp41 peptide) WNWFNITN gp41 peptide QELLELDKWASLWNWFNITN a c a a g a a t t a t t g g a a t t a g a t a a a t g g g c a a g t t t g t g g a a t t g g t t t a a c a t a a c a a a t g t t c t t a a t a a c c t t a a t c t a t t t a c c c g t t c a a a c a c c t t a a c c a a a t t g t a t t g t t t

1620 (in frame with gp41 peptide) WLWYIKLFIMIVGGLVGLRI WLWYIKLFIMIVGGLVGLRI t t g g c t g t g g t a t a t a a a a t t a t t c a t a a t g a t a g t a g g a g g c t t g g t a g g t t t a a g a a t a a c c g a c a c c a t a t a t t t t a a t a a g t a t t a c t a t c a t c c t c c g a a c c a t c c a a a t t c t t a

1680 (in frame with gp41 peptide) VFAVLSIVNRVRQGYSPLSF VFAVLSIVNRVRQGYSPLSF a g t t t t t g c t g t a c t t t c t a t a g t g a a t a g a g t t a g g c a g g g a t a t t c a c c a t t a t c g t t t c a a a a a c g a c a t g a a a g a t a t c a c t t a t c t c a a t c c g t c c c t a t a a g t g g t a a t a g c a a

1740 (in frame with gp41 peptide) QTHLPTPRGPDRPEGIEEEG QTHLPTPRGPDRPEGIEEEG t c a g a c c c a c c t c c c a a c c c c g a g g g g a c c c g a c a g g c c c g a a g g a a t a g a a g a a g a a g g a g t c t g g g t g g a g g g t t g g g g c t c c c c t g g g c t g t c c g g g c t t c c t t a t c t t c t t c t t c c

1800 (in frame with gp41 peptide) GERDRDRSIRLVNGSALRAP GERDRDRSIRLVNGSALRAP t g g a g a g a g a g a c a g a g a c a g a t c c a t t c g a t t a g t g a a c g g a t c g g c a c t g c g t g c g c c a c c t c t c t c t c t g t c t c t g t c t a g g t a a g c t a a t c a c t t g c c t a g c c g t g a c g c a c g c g g

1860 (in frame with gp41 peptide) ILQTNGSIHPQF* cPPT/CTS ILQTNGSIHPQF* a a t t c t g c a g a c a a a t g g c a g t a t t c a t c c a c a a t t t t a a a a g a a a a g g g g g g a t t g g g g t t a a g a c g t c t g t t t a c c g t c a t a a g t a g g t g t t a a a a t t t t c t t t t c c c c c c t a a c c c c

1920 cPPT/CTS g g t a c a g t g c a g g g g a a a g a a t a g t a g a c a t a a t a g c a a c a g a c a t a c a a a c t a a a g a a t c c a t g t c a c g t c c c c t t t c t t a t c a t c t g t a t t a t c g t t g t c t g t a t g t t t g a t t t c t t a

1980 cPPT/CTS t a c a a a a a c a a a t t a c a a a a a t t c a a a a t t t t c g g g t t t a t t a c a g g g a c a g c a g a g a t c a t g t t t t t g t t t a a t g t t t t t a a g t t t t a a a a g c c c a a a t a a t g t c c c t g t c g t c t c t a g

PacI 2040 UbC promoter c a g t t t g g t t a a t t a a c c c g t g t c g g c t c c a g a t c t g g c c t c c g c g c c g g g t t t t g g c g c g t c a a a c c a a t t a a t t g g g c a c a g c c g a g g t c t a g a c c g g a g g c g c g g c c c a a a a c c g c g

2100 UbC promoter c t c c c g c g g g c g c c c c c c t c c t c a c g g c g a g c g c t g c c a c g t c a g a c g a a g g g c g c a g c g g a g g g c g c c c g c g g g g g g a g g a g t g c c g c t c g c g a c g g t g c a g t c t g c t t c c c g c g t c g c

2160 UbC promoter a g c g t c c t g a t c c t t c c g c c c g g a c g c t c a g g a c a g c g g c c c g c t g c t c a t a a g a c t c g g t c g c a g g a c t a g g a a g g c g g g c c t g c g a g t c c t g t c g c c g g g c g a c g a g t a t t c t g a g c c

2220 UbC promoter c c t t a g a a c c c c a g t a t c a g c a g a a g g a c a t t t t a g g a c g g g a c t t g g g t g a c t c t a g g g g g a a t c t t g g g g t c a t a g t c g t c t t c c t g t a a a a t c c t g c c c t g a a c c c a c t g a g a t c c c

2280 UbC promoter c a c t g g t t t t c t t t c c a g a g a g c g g a a c a g g c g a g g a a a a g t a g t c c c t t c t c g g c g a t t g t g a c c a a a a g a a a g g t c t c t c g c c t t g t c c g c t c c t t t t c a t c a g g g a a g a g c c g c t a a

2340 UbC promoter c t g c g g a g g g a t c t c c g t g g g g c g g t g a a c g c c g a t g a t t a t a t a a g g a c g c g c c g g g t g g a c g c c t c c c t a g a g g c a c c c c g c c a c t t g c g g c t a c t a a t a t a t t c c t g c g c g g c c c a c

2400 UbC promoter t g g c a c a g c t a g t t c c g t c g c a g c c g g g a t t t g g g t c g c g g t t c t t g t t t g t g g a t c g c t a c c g t g t c g a t c a a g g c a g c g t c g g c c c t a a a c c c a g c g c c a a g a a c a a a c a c c t a g c g a

2460 UbC promoter g t g a t c g t c a c t t g g t g a g t a g c g g g c t g c t g g g c t g g c c g g g g c t t t c g t g g c c g c c g g c a c t a g c a g t g a a c c a c t c a t c g c c c g a c g a c c c g a c c g g c c c c g a a a g c a c c g g c g g c c

2520 UbC promoter g c c g c t c g g t g g g a c g g a a g c g t g t g g a g a g a c c g c c a a g g g c t g t a g t c t g g g t c c g c g c g g c g a g c c a c c c t g c c t t c g c a c a c c t c t c t g g c g g t t c c c g a c a t c a g a c c c a g g c g c

2580 UbC promoter a g c a a g g t t g c c c t g a a c t g g g g g t t g g g g g g a g c g c a g c a a a a t g g c g g c t g t t c c c g a t c g t t c c a a c g g g a c t t g a c c c c c a a c c c c c c t c g c g t c g t t t t a c c g c c g a c a a g g g c t

2640 UbC promoter g t c t t g a a t g g a a g a c g c t t g t g a g g c g g g c t g t g a g g t c g t t g a a a c a a g g t g g g g g g c c a g a a c t t a c c t t c t g c g a a c a c t c c g c c c g a c a c t c c a g c a a c t t t g t t c c a c c c c c c g

2700 UbC promoter a t g g t g g g c g g c a a g a a c c c a a g g t c t t g a g g c c t t c g c t a a t g c g g g a a a g c t c t t a t t t a c c a c c c g c c g t t c t t g g g t t c c a g a a c t c c g g a a g c g a t t a c g c c c t t t c g a g a a t a a

2760 UbC promoter c g g g t g a g a t g g g c t g g g g c a c c a t c t g g g g a c c c t g a c g t g a a g t t t g t c a c t g a c t g g g c c c a c t c t a c c c g a c c c c g t g g t a g a c c c c t g g g a c t g c a c t t c a a a c a g t g a c t g a c c

2820 UbC promoter a g a a c t c g g t t t g t c g t c t g t t g c g g g g g c g g c a g t t a t g g c g g t g c c g t t g g g c a g t g c t c t t g a g c c a a a c a g c a g a c a a c g c c c c c g c c g t c a a t a c c g c c a c g g c a a c c c g t c a c g

2880 UbC promoter a c c c g t a c c t t t g g g a g c g c g c g c c c t c g t c g t g t c g t g a c g t c a c c c g t t c t g t t g g c t t g g g c a t g g a a a c c c t c g c g c g c g g g a g c a g c a c a g c a c t g c a g t g g g c a a g a c a a c c g a

2940 UbC promoter t a t a a t g c a g g g t g g g g c c a c c t g c c g g t a g g t g t g c g g t a g g c t t t t c t c c g t c g c a g g a t a t t a c g t c c c a c c c c g g t g g a c g g c c a t c c a c a c g c c a t c c g a a a a g a g g c a g c g t c c

3000 UbC promoter a c g c a g g g t t c g g g c c t a g g g t a g g c t c t c c t g a a t c g a c a g g c g c c g g a c c t c t g g t g a t g c g t c c c a a g c c c g g a t c c c a t c c g a g a g g a c t t a g c t g t c c g c g g c c t g g a g a c c a c t

3060 UbC promoter g g g g a g g g a t a a g t g a g g c g t c a g t t t c t t t g g t c g g t t t t a t g t a c c t a t c t t c t t a a g c c c c t c c c t a t t c a c t c c g c a g t c a a a g a a a c c a g c c a a a a t a c a t g g a t a g a a g a a t t c

3120 UbC promoter T G A A G C T C C G G T T T T G A A C T hUBCpro-F t a g c t g a a g c t c c g g t t t t g a a c t a t g c g c t c g g g g t t g g c g a g t g t g t t t t g t g a a g t t a t c g a c t t c g a g g c c a a a a c t t g a t a c g c g a g c c c c a a c c g c t c a c a c a a a a c a c t t c a a

SpeI 3180 UbC promoter t t t t a g g c a c c t t t t g a a a t g t a a t c a t t t g g g t c a a t a t g t a a t t t t c a g t g t t a g a c t a a a a t c c g t g g a a a a c t t t a c a t t a g t a a a c c c a g t t a t a c a t t a a a a g t c a c a a t c t g a

3240 UbC promoter a g t a a a t t g t c c g c t a a a t t c t g g c c g t t t t t g g c t t t t t t g t t a g a c g a a g c t t g g g c t t c a t t t a a c a g g c g a t t t a a g a c c g g c a a a a a c c g a a a a a a c a a t c t g c t t c g a a c c c g a

EcoRVSgrAI 3300 1 5 ATNFSLLK P2A MGSGATNFSLLK g c a g g t c c g a t c c a c c g g t g a t a t c a t g g g a t c t g g a g c a a c a a a c t t c t c a c t a c t c a a c g t c c a g g c t a g g t g g c c a c t a t a g t a c c c t a g a c c t c g t t g t t t g a a g a g t g a t g a g t t

XbaI 3360 10 15 QAGDVEENPGP SR 1 5 MPEPAKS P2A H2B QAGDVEENPGPSRMPEPAKS a c a a g c a g g t g a c g t g g a g g a g a a t c c c g g a c c t t c t a g a a t g c c a g a g c c a g c g a a g t c t g t t c g t c c a c t g c a c c t c c t c t t a g g g c c t g g a a g a t c t t a c g g t c t c g g t c g c t t c a g

3420 10 15 20 25 APAPKKGSKKAVTKAQKKGG H2B APAPKKGSKKAVTKAQKKGG t g c t c c c g c c c c g a a a a a g g g c t c c a a g a a g g c g g t g a c t a a g g c g c a g a a g a a a g g c g g a c g a g g g c g g g g c t t t t t c c c g a g g t t c t t c c g c c a c t g a t t c c g c g t c t t c t t t c c g c c

3480 30 35 40 45 KKRKRSRKESYSIYVYKVLK H2B KKRKRSRKESYSIYVYKVLK c a a g a a g c g c a a g c g c a g c c g c a a g g a g a g c t a t t c c a t c t a t g t g t a c a a g g t t c t g a a g t t c t t c g c g t t c g c g t c g g c g t t c c t c t c g a t a a g g t a g a t a c a c a t g t t c c a a g a c t t

BstXI 3540 50 55 60 65 QVHPDTGISSKAMGIMNSFV H2B QVHPDTGISSKAMGIMNSFV g c a g g t c c a c c c t g a c a c c g g c a t t t c g t c c a a g g c c a t g g g c a t c a t g a a t t c g t t t g t c g t c c a g g t g g g a c t g t g g c c g t a a a g c a g g t t c c g g t a c c c g t a g t a c t t a a g c a a a c a

3600 70 75 80 85 NDIFERIAGEASRLAHYNKR H2B NDIFERIAGEASRLAHYNKR g a a c g a c a t t t t c g a g c g c a t c g c a g g t g a g g c t t c c c g c c t g g c g c a t t a c a a c a a g c g c t t g c t g t a a a a g c t c g c g t a g c g t c c a c t c c g a a g g g c g g a c c g c g t a a t g t t g t t c g c

3660 90 95 100 105 STITSREIQTAVRLLLPGEL H2B STITSREIQTAVRLLLPGEL c t c g a c c a t c a c c t c c a g g g a g a t c c a g a c g g c c g t g c g c c t g c t g c t g c c t g g g g a g t t g a g c t g g t a g t g g a g g t c c c t c t a g g t c t g c c g g c a c g c g g a c g a c g a c g g a c c c c t c a a

BamHIMscI 3720 110 115 120 125 AKHAVSEGTKAITKYTSAK D H2B AKHAVSEGTKAITKYTSAKD g g c c a a g c a c g c c g t g t c c g a g g g t a c t a a g g c c a t c a c c a a g t a c a c c a g c g c t a a g g a c c g g t t c g t g c g g c a c a g g c t c c c a t g a t t c c g g t a g t g g t t c a t g t g g t c g c g a t t c c t

3780 (in frame with H2B) PPVAT 1 M 1a V 5 10 SKGEELFTGVVPI EGFP 1 M 1a V 5 10 SKGEELFTGVVPI EGFP Kozak sequence PPVATMVSKGEELFTGVVPI t c c a c c g g t c g c c a c c a t g g t g a g c a a g g g c g a g g a g c t g t t c a c c g g g g t g g t g c c c a t a g g t g g c c a g c g g t g g t a c c a c t c g t t c c c g c t c c t c g a c a a g t g g c c c c a c c a c g g g t a

3840 15 20 25 30 LVELDGDVNGHKFSVSGEGE EGFP 15 20 25 30 LVELDGDVNGHKFSVSGEGE EGFP G A C C A G C T C G A C C T G C C G C T G C EGFP-N LVELDGDVNGHKFSVSGEGE c c t g g t c g a g c t g g a c g g c g a c g t a a a c g g c c a c a a g t t c a g c g t g t c c g g c g a g g g c g a g g a c c a g c t c g a c c t g c c g c t g c a t t t g c c g g t g t t c a a g t c g c a c a g g c c g c t c c c g c t

3900 35 40 45 50 GDATYGKLTLKFICTTGKLP EGFP 35 40 45 50 GDATYGKLTLKFICTTGKLP EGFP GDATYGKLTLKFICTTGKLP g g g c g a t g c c a c c t a c g g c a a g c t g a c c c t g a a g t t c a t c t g c a c c a c c g g c a a g c t g c c c c c g c t a c g g t g g a t g c c g t t c g a c t g g g a c t t c a a g t a g a c g t g g t g g c c g t t c g a c g g

3960 55 60 65 70 VPWPTLVTTLTYGVQCFSRY EGFP 55 60 65 70 VPWPTLVTTLTYGVQCFSRY EGFP VPWPTLVTTLTYGVQCFSRY c g t g c c c t g g c c c a c c c t c g t g a c c a c c c t g a c c t a c g g c g t g c a g t g c t t c a g c c g c t a g c a c g g g a c c g g g t g g g a g c a c t g g t g g g a c t g g a t g c c g c a c g t c a c g a a g t c g g c g a t

4020 75 80 85 90 PDHMKQHDFFKSAMPEGYVQ EGFP 75 80 85 90 PDHMKQHDFFKSAMPEGYVQ EGFP PDHMKQHDFFKSAMPEGYVQ c c c c g a c c a c a t g a a g c a g c a c g a c t t c t t c a a g t c c g c c a t g c c c g a a g g c t a c g t c c a g g g g c t g g t g t a c t t c g t c g t g c t g a a g a a g t t c a g g c g g t a c g g g c t t c c g a t g c a g g t

4080 95 100 105 110 ERTIFFKDDGNYKTRAEVKF EGFP 95 100 105 110 ERTIFFKDDGNYKTRAEVKF EGFP C T G C T G C C G T T G A T G T T C T G EXFP-R ERTIFFKDDGNYKTRAEVKF g g a g c g c a c c a t c t t c t t c a a g g a c g a c g g c a a c t a c a a g a c c c g c g c c g a g g t g a a g t t c c t c g c g t g g t a g a a g a a g t t c c t g c t g c c g t t g a t g t t c t g g g c g c g g c t c c a c t t c a a

4140 115 120 125 130 EGDTLVNRIELKGIDFKEDG EGFP 115 120 125 130 EGDTLVNRIELKGIDFKEDG EGFP EGDTLVNRIELKGIDFKEDG c g a g g g c g a c a c c c t g g t g a a c c g c a t c g a g c t g a a g g g c a t c g a c t t c a a g g a g g a c g g g c t c c c g c t g t g g g a c c a c t t g g c g t a g c t c g a c t t c c c g t a g c t g a a g t t c c t c c t g c c

4200 135 140 145 150 NILGHKLEYNYNSHNVYIMA EGFP 135 140 145 150 NILGHKLEYNYNSHNVYIMA EGFP NILGHKLEYNYNSHNVYIMA c a a c a t c c t g g g g c a c a a g c t g g a g t a c a a c t a c a a c a g c c a c a a c g t c t a t a t c a t g g c g t t g t a g g a c c c c g t g t t c g a c c t c a t g t t g a t g t t g t c g g t g t t g c a g a t a t a g t a c c g

4260 155 160 165 170 DKQKNGIKVNFKIRHNIEDG EGFP 155 160 165 170 DKQKNGIKVNFKIRHNIEDG EGFP DKQKNGIKVNFKIRHNIEDG c g a c a a g c a g a a g a a c g g c a t c a a g g t g a a c t t c a a g a t c c g c c a c a a c a t c g a g g a c g g g c t g t t c g t c t t c t t g c c g t a g t t c c a c t t g a a g t t c t a g g c g g t g t t g t a g c t c c t g c c

4320 175 180 185 190 SVQLADHYQQNTPIGDGPVL EGFP 175 180 185 190 SVQLADHYQQNTPIGDGPVL EGFP SVQLADHYQQNTPIGDGPVL c a g c g t g c a g c t c g c c g a c c a c t a c c a g c a g a a c a c c c c c a t c g g c g a c g g c c c c g t g c t g t c g c a c g t c g a g c g g c t g g t g a t g g t c g t c t t g t g g g g g t a g c c g c t g c c g g g g c a c g a

4380 195 200 205 210 LPDNHYLSTQSALSKDPNEK EGFP 195 200 205 210 LPDNHYLSTQSALSKDPNEK EGFP LPDNHYLSTQSALSKDPNEK g c t g c c c g a c a a c c a c t a c c t g a g c a c c c a g t c c g c c c t g a g c a a a g a c c c c a a c g a g a a c g a c g g g c t g t t g g t g a t g g a c t c g t g g g t c a g g c g g g a c t c g t t t c t g g g g t t g c t c t t

4440 215 220 225 230 RDHMVLLEFVTAAGITLGMD EGFP 215 220 225 230 RDHMVLLEFVTAAGITLGMD EGFP C A T G G T C C T G C T G G A G T T C G T G EGFP-C RDHMVLLEFVTAAGITLGMD g c g c g a t c a c a t g g t c c t g c t g g a g t t c g t g a c c g c c g c c g g g a t c a c t c t c g g c a t g g a c g c g c t a g t g t a c c a g g a c g a c c t c a a g c a c t g g c g g c g g c c c t a g t g a g a g c c g t a c c t

4500 235 ELYK (in frame with EGFP) RSGSG 140 145 EGRGSLLTCGD EGFP T2A 235 ELYK (in frame with EGFP) RSGSGEGRGSLLTCGD EGFP ELYKRSGSGEGRGSLLTCGD c g a g c t g t a c a a g a g a t c c g g c t c c g g c g a g g g c a g g g g a a g t c t a c t a a c a t g c g g g g a g c t c g a c a t g t t c t c t a g g c c g a g g c c g c t c c c g t c c c c t t c a g a t g a t t g t a c g c c c c t

SalIHincIIBspEIBclI*BmtINheI 4560 (in frame with EGFP) VEENPGPASV* 150 155 VEENPGP (in frame with T2A) ASV* T2A VEENPGPASV* c g t g g a g g a a a a t c c c g g c c c a g c t a g c g t g t g a t c a g a a t t c g t t c c g g a g t c g t c g a c g c a c c t c c t t t t a g g g c c g g g t c g a t c g c a c a c t a g t c t t a a g c a a g g c c t c a g c a g c t g

4620 WPRE A C WPRE-R t c g a c a a t c a a c c t c t g g a t t a c a a a a t t t g t g a a a g a t t g a c t g g t a t t c t t a a c t a t g a g c t g t t a g t t g g a g a c c t a a t g t t t t a a a c a c t t t c t a a c t g a c c a t a a g a a t t g a t a c

4680 WPRE (in frame with Factor Xa site) *HRQIMSNS A A C G A G G A A A A T G C G A T A C WPRE-R MPLYHAIA *HRQIMSNS t t g c t c c t t t t a c g c t a t g t g g a t a c g c t g c t t t a a t g c c t t t g t a t c a t g c t a t t g c t t a a c g a g g a a a a t g c g a t a c a c c t a t g c g a c g a a a t t a c g g a a a c a t a g t a c g a t a a c g a a

4740 WPRE (in frame with Factor Xa site) GTHSENEGGQIFGPQQRKIL SRMAFIFSSLYKSWLLSLYE GTHSENEGGQIFGPQQRKIL c c c g t a t g g c t t t c a t t t t c t c c t c c t t g t a t a a a t c c t g g t t g c t g t c t c t t t a t g a g g g g g c a t a c c g a a a g t a a a a g a g g a g g a a c a t a t t t a g g a c c a a c g a c a g a g a a a t a c t c c

4800 WPRE (in frame with Factor Xa site) LQPGNDPLTAHHASHKSVCG ELWPVVRQRGVVCTVFADAT LQPGNDPLTAHHASHKSVCG a g t t g t g g c c c g t t g t c a g g c a a c g t g g c g t g g t g t g c a c t g t g t t t g c t g a c g c a a c c c t c a a c a c c g g g c a a c a g t c c g t t g c a c c g c a c c a c a c g t g a c a c a a a c g a c t g c g t t g g g

PflMI 4860 WPRE (in frame with Factor Xa site) GSTPANGGGTLEKGPSESEG PTGWGIATTCQLLSGTFAFP GSTPANGGGTLEKGPSESEG c c a c t g g t t g g g g c a t t g c c a c c a c c t g t c a g c t c c t t t c c g g g a c t t t c g c t t t c c c c c g g t g a c c a a c c c c g t a a c g g t g g t g g a c a g t c g a g g a a a g g c c c t g a a a g c g a a a g g g g g

4920 WPRE (in frame with Factor Xa site) ERNGRRFEDGGAKGAAPCPS LPIATAELIAACLARCWTGA ERNGRRFEDGGAKGAAPCPS t c c c t a t t g c c a c g g c g g a a c t c a t c g c c g c c t g c c t t g c c c g c t g c t g g a c a g g g g c t c a g g g a t a a c g g t g c c g c c t t g a g t a g c g g c g g a c g g a a c g g g c g a c g a c c t g t c c c c g a g

4980 WPRE (in frame with Factor Xa site) PQQASVIGHHQRPLQRGKWP RLLGTDNSVVLSGKLTSFPW PQQASVIGHHQRPLQRGKWP g g c t g t t g g g c a c t g a c a a t t c c g t g g t g t t g t c g g g g a a g c t g a c g t c c t t t c c a t g g c c c g a c a a c c c g t g a c t g t t a a g g c a c c a c a a c a g c c c c t t c g a c t g c a g g a a a g g t a c c g

5040 WPRE (in frame with Factor Xa site) QEGTNGGPNQAPRGEAVDR R Factor Xa site LLACVATWILRGTSFCYVPS QEGTNGGPNQAPRGEAVDRR t g c t c g c c t g t g t t g c c a c c t g g a t t c t g c g c g g g a c g t c c t t c t g c t a c g t c c c t t c g g a c g a g c g g a c a c a a c g g t g g a c c t a a g a c g c g c c c t g c a g g a a g a c g a t g c a g g g a a g c c

5100 WPRE 1 GEI Factor Xa site ALNPADLPSRGLLPALRPLP GEIWRVKRGAAQQRSQPRKR c c c t c a a t c c a g c g g a c c t t c c t t c c c g c g g c c t g c t g c c g g c t c t g c g g c c t c t t c c g c g g g a g t t a g g t c g c c t g g a a g g a a g g g c g c c g g a c g a c g g c c g a g a c g c c g g a g a a g g c g

5160 WPRE HLRLRPQTSRISLWAASPPG M a t c t t c g c c t t c g c c c t c a g a c g a g t c g g a t c t c c c t t t g g g c c g c c t c c c c g c c t g g a a t a g a a g c g g a a g c g g g a g t c t g c t c a g c c t a g a g g g a a a c c c g g c g g a g g g g c g g a c c t t

Acc65IKpnI 5220 INSSSVPLRPMTYKAAVDLS t t a a t t c g a g c t c g g t a c c t t t a a g a c c a a t g a c t t a c a a g g c a g c t g t a g a t c t t a g c c a a t t a a g c t c g a g c c a t g g a a a t t c t g g t t a c t g a a t g t t c c g t c g a c a t c t a g a a t c g g

SnaBI 5280 3' LTR ( Δ U3) HFLKEKGGLEGLRNSQRRQD a c t t t t t a a a a g a a a a g g g g g g a c t g g a a g g g c t a c g t a a c t c c c a a c g a a g a c a a g a t c t g a a a a a t t t t c t t t t c c c c c c t g a c c t t c c c g a t g c a t t g a g g g t t g c t t c t g t t c t a g

5340 3' LTR ( Δ U3) LLFACTGSLWLDQI* t g c t t t t t g c t t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g a c g a a a a a c g a a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c

5400 3' LTR ( Δ U3) g c t a a c t a g g g a a c c c a c t g c t t a a g c c t c a a t a a a g c t t g c c t t g a g t g c t t c a a g t a g c g a t t g a t c c c t t g g g t g a c g a a t t c g g a g t t a t t t c g a a c g g a a c t c a c g a a g t t c a t c

5460 3' LTR ( Δ U3) t g t g t g c c c g t c t g t t g t g t g a c t c t g g t a a c t a g a g a t c c c t c a g a c c c t t t t a g t c a g a c a c a c g g g c a g a c a a c a c a c t g a g a c c a t t g a t c t c t a g g g a g t c t g g g a a a a t c a g t c

5520 3' LTR ( Δ U3) t g t g g a a a a t c t c t a g c a g t a g t a g t t c a t g t c a t c t t a t t a t t c a g t a t t t a t a a c t t g a c a c c t t t t a g a g a t c g t c a t c a t c a a g t a c a g t a g a a t a a t a a g t c a t a a a t a t t g a a c

5580 SV40 poly(A) signal c a a a g a a a t g a a t a t c a g a g a g t g a g a g g a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a g t t t c t t t a c t t a t a g t c t c t c a c t c t c c t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t

5640 SV40 poly(A) signal C G T T A T C G T A G T G T T T A A A G SV40pA-R a a t a a a g c a a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t t t a t t t c g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a

5700 SV40 poly(A) signal G T G G T T T G T C C A A A C T C A T C EBV-rev g t g g t t t g t c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g c t c t a g c t a t c c c g c c c c t c a c c a a a c a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c g a g a t c g a t a g g g c g g g g a

5760 SV40 ori a a c t c c g c c c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g t t g a g g c g g g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c

SfiI 5820 SV40 ori a c t a a t t t t t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a t g a t t a a a a a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t

5880 SV40 ori T7 promoter G G G A T A T C A C T C A G C T7 g t a g t g a g g a g g c t t t t t t g g a g g c c t a g g g a c g t a c c c a a t t c g c c c t a t a g t g a g t c g c a t c a c t c c t c c g a a a a a a c c t c c g g a t c c c t g c a t g g g t t a a g c g g g a t a t c a c t c a g c

5940 T7 promoter M13 fwd A T A A T T7 T G A C C G G C A G C A A A A T G T M13 Forward G C A A A A T G T T G C A G C A C T G A C C C M13/pUC Forward t a t t a c g c g c g c t c a c t g g c c g t c g t t t t a c a a c g t c g t g a c t g g g a a a a c c c t g g c g t t a t a a t g c g c g c g a g t g a c c g g c a g c a a a a t g t t g c a g c a c t g a c c c t t t t g g g a c c g c a a

6000 a c c c a a c t t a a t c g c c t t g c a g c a c a t c c c c c t t t c g c c a g c t g g c g t a a t a g c g a a g a g t g g g t t g a a t t a g c g g a a c g t c g t g t a g g g g g a a a g c g g t c g a c c g c a t t a t c g c t t c t c

6060 f1 ori g c c c g c a c c g a t c g c c c t t c c c a a c a g t t g c g c a g c c t g a a t g g c g a a t g g c g c g a c g c g c g g g c g t g g c t a g c g g g a a g g g t t g t c a a c g c g t c g g a c t t a c c g c t t a c c g c g c t g c g c

6120 f1 ori c c c t g t a g c g g c g c a t t a a g c g c g g c g g g t g t g g t g g t t a c g c g c a g c g t g a c c g c t a c a g g g a c a t c g c c g c g t a a t t c g c g c c g c c c a c a c c a c c a a t g c g c g t c g c a c t g g c g a t g t

6180 f1 ori G A G G A A A G C G A A A G A A G G G A F1ori-R c t t g c c a g c g c c c t a g c g c c c g c t c c t t t c g c t t t c t t c c c t t c c t t t c t c g c c a c g t t c g a a c g g t c g c g g g a t c g c g g g c g a g g a a a g c g a a a g a a g g g a a g g a a a g a g c g g t g c a a g

6240 f1 ori g c c g g c t t t c c c c g t c a a g c t c t a a a t c g g g g g c t c c c t t t a g g g t t c c g a t t t a g t g c t c g g c c g a a a g g g g c a g t t c g a g a t t t a g c c c c c g a g g g a a a t c c c a a g g c t a a a t c a c g a

6300 f1 ori t t a c g g c a c c t c g a c c c c a a a a a a c t t g a t t a g g g t g a t g g t t c a c g t a g t g g g c c a t c g a a t g c c g t g g a g c t g g g g t t t t t t g a a c t a a t c c c a c t a c c a a g t g c a t c a c c c g g t a g c

6360 f1 ori G T G G A C T C F1ori-F c c c t g a t a g a c g g t t t t t c g c c c t t t g a c g t t g g a g t c c a c g t t c t t t a a t a g t g g a c t c g g g a c t a t c t g c c a a a a a g c g g g a a a c t g c a a c c t c a g g t g c a a g a a a t t a t c a c c t g a g

6420 f1 ori T T G T T C C A A A C T G G F1ori-F t t g t t c c a a a c t g g a a c a a c a c t c a a c c c t a t c t c g g t c t a t t c t t t t g a t t t a t a a g g g a a c a a g g t t t g a c c t t g t t g t g a g t t g g g a t a g a g c c a g a t a a g a a a a c t a a a t a t t c c c

6480 f1 ori a t t t t g c c g a t t t c g g c c t a t t g g t t a a a a a a t g a g c t g a t t t a a c a a a a a t t t a a c g c g t a a a a c g g c t a a a g c c g g a t a a c c a a t t t t t t a c t c g a c t a a a t t g t t t t t a a a t t g c g c

6540 f1 ori AmpR promoter a a t t t t a a c a a a a t a t t a a c g t t t a c a a t t t c c c a g g t g g c a c t t t t c g g g g a a a t g t g c t t a a a a t t g t t t t a t a a t t g c a a a t g t t a a a g g g t c c a c c g t g a a a a g c c c c t t t a c a c g

6600 AmpR promoter g c g g a a c c c c t a t t t g t t t a t t t t t c t a a a t a c a t t c a a a t a t g t a t c c g c t c a t g a g a c c g c c t t g g g g a t a a a c a a a t a a a a a g a t t t a t g t a a g t t t a t a c a t a g g c g a g t a c t c t g

6660 1 5 signal sequence MSIQH AmpR promoter AmpR MSIQH a a t a a c c c t g a t a a a t g c t t c a a t a a t a t t g a a a a a g g a a g a g t a t g a g t a t t c a a c a t t t t a t t g g g a c t a t t t a c g a a g t t a t t a t a a c t t t t t c c t t c t c a t a c t c a t a a g t t g t a a

6720 10 15 20 signal sequence FRVALIPFFAAFCLPVFA 25 HP AmpR FRVALIPFFAAFCLPVFAHP t c c g t g t c g c c c t t a t t c c c t t t t t t g c g g c a t t t t g c c t t c c t g t t t t t g c t c a c c c a g a g g c a c a g c g g g a a t a a g g g a a a a a a c g c c g t a a a a c g g a a g g a c a a a a a c g a g t g g g t c

6780 30 35 40 45 ETLVKVKDAEDQLGARVGYI AmpR ETLVKVKDAEDQLGARVGYI a a a c g c t g g t g a a a g t a a a a g a t g c t g a a g a t c a g t t g g g t g c a c g a g t g g g t t a c a t c g t t t g c g a c c a c t t t c a t t t t c t a c g a c t t c t a g t c a a c c c a c g t g c t c a c c c a a t g t a g c

6840 50 55 60 65 ELDLNSGKILESFRPEERFP AmpR ELDLNSGKILESFRPEERFP a a c t g g a t c t c a a c a g c g g t a a g a t c c t t g a g a g t t t t c g c c c c g a a g a a c g t t t t c c a a t t g a c c t a g a g t t g t c g c c a t t c t a g g a a c t c t c a a a a g c g g g g c t t c t t g c a a a a g g t t

6900 70 75 80 85 MMSTFKVLLCGAVLSRIDAG AmpR C G A T A C A C C G C G C C A T A A T A Amp-R MMSTFKVLLCGAVLSRIDAG t g a t g a g c a c t t t t a a a g t t c t g c t a t g t g g c g c g g t a t t a t c c c g t a t t g a c g c c g g g c a c t a c t c g t g a a a a t t t c a a g a c g a t a c a c c g c g c c a t a a t a g g g c a t a a c t g c g g c c c g

ScaI 6960 90 95 100 105 QEQLGRRIHYSQNDLVEYSP AmpR QEQLGRRIHYSQNDLVEYSP a a g a g c a a c t c g g t c g c c g c a t a c a c t a t t c t c a g a a t g a c t t g g t t g a g t a c t c a c c a g t t c t c g t t g a g c c a g c g g c g t a t g t g a t a a g a g t c t t a c t g a a c c a a c t c a t g a g t g g t c

7020 110 115 120 125 VTEKHLTDGMTVRELCSAAI AmpR VTEKHLTDGMTVRELCSAAI t c a c a g a a a a g c a t c t t a c g g a t g g c a t g a c a g t a a g a g a a t t a t g c a g t g c t g c c a t a a a g t g t c t t t t c g t a g a a t g c c t a c c g t a c t g t c a t t c t c t t a a t a c g t c a c g a c g g t a t t

7080 130 135 140 145 TMSDNTAANLLLTTIGGPKE AmpR TMSDNTAANLLLTTIGGPKE c c a t g a g t g a t a a c a c t g c g g c c a a c t t a c t t c t g a c a a c g a t c g g a g g a c c g a a g g a g c g g t a c t c a c t a t t g t g a c g c c g g t t g a a t g a a g a c t g t t g c t a g c c t c c t g g c t t c c t c g

7140 150 155 160 165 LTAFLHNMGDHVTRLDRWEP AmpR LTAFLHNMGDHVTRLDRWEP t a a c c g c t t t t t t g c a c a a c a t g g g g g a t c a t g t a a c t c g c c t t g a t c g t t g g g a a c c g g a t t g g c g a a a a a a c g t g t t g t a c c c c c t a g t a c a t t g a g c g g a a c t a g c a a c c c t t g g c c

7200 170 175 180 185 ELNEAIPNDERDTTMPVAMA AmpR ELNEAIPNDERDTTMPVAMA a g c t g a a t g a a g c c a t a c c a a a c g a c g a g c g t g a c a c c a c g a t g c c t g t a g c a a t g g c a a t c g a c t t a c t t c g g t a t g g t t t g c t g c t c g c a c t g t g g t g c t a c g g a c a t c g t t a c c g t t

7260 190 195 200 205 TTLRKLLTGELLTLASRQQL AmpR TTLRKLLTGELLTLASRQQL c a a c g t t g c g c a a a c t a t t a a c t g g c g a a c t a c t t a c t c t a g c t t c c c g g c a a c a a t t a a g t t g c a a c g c g t t t g a t a a t t g a c c g c t t g a t g a a t g a g a t c g a a g g g c c g t t g t t a a t t

7320 210 215 220 225 IDWMEADKVAGPLLRSALPA AmpR IDWMEADKVAGPLLRSALPA t a g a c t g g a t g g a g g c g g a t a a a g t t g c a g g a c c a c t t c t g c g c t c g g c c c t t c c g g c t g a t c t g a c c t a c c t c c g c c t a t t t c a a c g t c c t g g t g a a g a c g c g a g c c g g g a a g g c c g a c

7380 230 235 240 245 GWFIADKSGAGERGSRGIIA AmpR GWFIADKSGAGERGSRGIIA g c t g g t t t a t t g c t g a t a a a t c t g g a g c c g g t g a g c g t g g g t c t c g c g g t a t c a t t g c a g c g a c c a a a t a a c g a c t a t t t a g a c c t c g g c c a c t c g c a c c c a g a g c g c c a t a g t a a c g t c

AhdI 7440 250 255 260 265 ALGPDGKPSRIVVIYTTGSQ AmpR ALGPDGKPSRIVVIYTTGSQ c a c t g g g g c c a g a t g g t a a g c c c t c c c g t a t c g t a g t t a t c t a c a c g a c g g g g a g t c a g g g t g a c c c c g g t c t a c c a t t c g g g a g g g c a t a g c a t c a a t a g a t g t g c t g c c c c t c a g t c c

7500 270 275 280 285 ATMDERNRQIAEIGASLIKH AmpR ATMDERNRQIAEIGASLIKH c a a c t a t g g a t g a a c g a a a t a g a c a g a t c g c t g a g a t a g g t g c c t c a c t g a t t a a g c a t t g t t g a t a c c t a c t t g c t t t a t c t g t c t a g c g a c t c t a t c c a c g g a g t g a c t a a t t c g t a a

7560 W* AmpR W* g g t a a c t g t c a g a c c a a g t t t a c t c a t a t a t a c t t t a g a t t g a t t t a a a a c t t c a t t t t t c c a t t g a c a g t c t g g t t c a a a t g a g t a t a t a t g a a a t c t a a c t a a a t t t t g a a g t a a a a a

7620 a a t t t a a a a g g a t c t a g g t g a a g a t c c t t t t t g a t a a t c t c a t g a c c a a a a t c c c t t a a c t t a a a t t t t c c t a g a t c c a c t t c t a g g a a a a a c t a t t a g a g t a c t g g t t t t a g g g a a t t g

7680 ori g t g a g t t t t c g t t c c a c t g a g c g t c a g a c c c c g t a g a a a a g a t c a a a g g a t c t t c t t g a g c a c t c a a a a g c a a g g t g a c t c g c a g t c t g g g g c a t c t t t t c t a g t t t c c t a g a a g a a c t c

7740 ori a t c c t t t t t t t c t g c g c g t a a t c t g c t g c t t g c a a a c a a a a a a a c c a c c g c t a c c a g c g g t a g g a a a a a a a g a c g c g c a t t a g a c g a c g a a c g t t t g t t t t t t t g g t g g c g a t g g t c g c c

7800 ori t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t t t c c g a a g g t a a c t g g c t t c a g c a a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g a a a a a g g c t t c c a t t g a c c g a a g t c g t

7860 ori g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a g c c g t a g t t a g g c c a c c a c t t c a a g a c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a t c g g c a t c a a t c c g g t g g t g a a g t t c t

7920 ori a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t a a t c c t g t t a c c a g t g g c t g c t g c c a t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g a t t a g g a c a a t g g t c a c c g a c g a c g g t

7980 ori g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c t c a a g a c g a t a g t t a c c g g a t a a g g c g c c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g a g t t c t g c t a t c a a t g g c c t a t t c c g c g

8040 ori a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g c c c a g c t t g g a g c g a a c g a c c t a c a t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g t c g g g t c g a a c c t c g c t t g c t g g a t g t

8100 ori c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g a a a g c g c c a c g c t t c c c g a a g g g a g a a g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c t t t c g c g g t g c g a a g g g c t t c c c t c t t

8160 ori a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g g a a c a g g a g a g c g c a c g a g g g a g c t t c t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c c t t g t c c t c t c g c g t g c t c c c t c g a a g

8220 ori G G G A A A C G C C T G G T A T C T T T pBR322ori-F c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c a g c c c a a a g c g g t g g a g a c t g a a c t c g

8280 ori g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a g c c t a t g g a a a a a c g c c a g c a a c g c g g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t c g g a t a c c t t t t t g c g g t c g t t g c g c c

AflIIIPciI 8340 c c t t t t t a c g g t t c c t g g c c t t t t g c t g g c c t t t t g c t c a c a t g t t c t t t c c t g c g t t a t g g a a a a a t g c c a a g g a c c g g a a a a c g a c c g g a a a a c g a g t g t a c a a g a a a g g a c g c a a t a

8400 c c c c t g a t t c t g t g g a t a a c c g t a t t a c c g c c t t t g a g t g a g c t g a t a c c g c t c g c c g c a g g g g a c t a a g a c a c c t a t t g g c a t a a t g g c g g a a a c t c a c t c g a c t a t g g c g a g c g g c g t

8460 A G C G A G T C A G T G A G C G A G L4440 g c c g a a c g a c c g a g c g c a g c g a g t c a g t g a g c g a g g a a g c g g a a g a g c g c c c a a t a c g c a c g g c t t g c t g g c t c g c g t c g c t c a g t c a c t c g c t c c t t c g c c t t c t c g c g g g t t a t g c g t

8520 a a c c g c c t c t c c c c g c g c g t t g g c c g a t t c a t t a a t g c a g c t g g c a c g a c a g g t t t c c c g t t g g c g g a g a g g g g c g c g c a a c c g g c t a a g t a a t t a c g t c g a c c g t g c t g t c c a a a g g g c

8580 CAP binding site a c t g g a a a g c g g g c a g t g a g c g c a a c g c a a t t a a t g t g a g t t a g c t c a c t c a t t a g g c a c t g a c c t t t c g c c c g t c a c t c g c g t t g c g t t a a t t a c a c t c a a t c g a g t g a g t a a t c c g t g

8640 -35 -10 lac promoter lac operator A G C G G A T A A C M13/pUC Reverse c c c a g g c t t t a c a c t t t a t g c t t c c g g c t c g t a t g t t g t g t g g a a t t g t g a g c g g a t a a c g g g t c c g a a a t g t g a a a t a c g a a g g c c g a g c a t a c a a c a c a c c t t a a c a c t c g c c t a t t g

8700 lac operator M13 rev T3 promoter A A T T T C A C A C A G G M13/pUC Reverse C A G G A A A C A G C T A T G A C M13 Reverse G C A A T T A A C C C T C A C T T3 a a t t t c a c a c a g g a a a c a g c t a t g a c c a t g a t t a c g c c a a g c g c g c a a t t a a c c c t c a c t t t a a a g t g t g t c c t t t g t c g a t a c t g g t a c t a a t g c g g t t c g c g c g t t a a t t g g g a g t g a

3ʹ 5ʹ 8724 T3 promoter A A A G G T3 a a a g g g a a c a a a a g c t g g a g c t g c t t t c c c t t g t t t t c g a c c t c g a c g

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.