Addgene pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03 Sequencing Result - Sequence Analyzer Skip to main content
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Sequence Analyzer: pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03 Sequencing Result


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HindIII (5245) AflII (5231) EcoRI (5095) SnaBI (4904) AseI (4571) SpeI (4563) MluI (4542) NruI (4522) TaqII (4485) SacII (4192) PshAI (4191) F1ori-R (3968 .. 3987) F1ori-F (3753 .. 3774) pBR322ori-F (3512 .. 3531) BssSI - BssSαI (3494) AvrII (3016) SfiI (2969) CsiI - SexAI * (2783) pBABE 3' (2697 .. 2717) BspDI * - ClaI * (2679) EBV-rev (2632 .. 2651) PaeR7I - XhoI (21) BbvCI (92) BstXI (122) Bsu36I (541) AccI (879) EcoO109I - PpuMI (902) EarI (909) NheI (1001) BmtI (1005) BamHI (1164) PasI (1385) EcoNI (1389) PvuI (1439) BspHI (1705) BmgBI (2063) PstI (2158) EagI - NotI (2398) DraI (2479) HpaI (2540) SV40pA-R (2578 .. 2597) pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03 5246 bp

Sequence View

PaeR7IXhoI 5ʹ 3ʹ 60 g c t t c t a g a g a t c c c t c g a c c t c g a g a t c c a t t g t g c t c t a a a g g a g a t a c c c g g c c a g a c g a a g a t c t c t a g g g a g c t g g a g c t c t a g g t a a c a c g a g a t t t c c t c t a t g g g c c g g t c t

BbvCI 120 M c a c c c t c a c c t g c g g t g c c c a g c t g c c c a g g c t g a g g c a a g a g a a g g c c a g a a a c c a t g c g t g g g a g t g g a c g c c a c g g g t c g a c g g g t c c g a c t c c g t t c t c t t c c g g t c t t t g g t a c g

BstXI 180 PMGSLQPLATLYLLGMLVAS c c a t g g g g t c t c t g c a a c c g c t g g c c a c c t t g t a c c t g c t g g g g a t g c t g g t c g c t t c c g g g t a c c c c a g a g a c g t t g g c g a c c g g t g g a a c a t g g a c g a c c c c t a c g a c c a g c g a a g g c

240 1 SA 5 10 Strep-Tag II WSHPQFEK 15 GGGSGGGS Twin-Strep-tag VDSAWSHPQFEKGGGSGGGS t g g a t t c t g c t t g g a g t c a c c c t c a g t t t g a a a a a g g c g g g g g g a g c g g g g g g g g g a g c g a c c t a a g a c g a a c c t c a g t g g g a g t c a a a c t t t t t c c g c c c c c c t c g c c c c c c c c c t c g c

300 20 GGSA 25 30 Strep-Tag II WSHPQFEK (in frame with Twin-Strep-tag) MSKGEELF Twin-Strep-tag GGSAWSHPQFEKMSKGEELF g g g g g a g t g c t t g g a g t c a c c c t c a g t t t g a a a a g a t g a g c a a g g g a g a g g a g c t g t t c a c c c c c t c a c g a a c c t c a g t g g g a g t c a a a c t t t t c t a c t c g t t c c c t c t c c t c g a c a a g t

360 (in frame with Twin-Strep-tag) TGVVPILVELDGDVNGHKFS TGVVPILVELDGDVNGHKFS c c g g a g t g g t g c c t a t c c t g g t g g a g c t g g a c g g c g a t g t g a a c g g c c a c a a g t t t t c c g g g c c t c a c c a c g g a t a g g a c c a c c t c g a c c t g c c g c t a c a c t t g c c g g t g t t c a a a a g g c

420 (in frame with Twin-Strep-tag) VRGEGEGDATNGKLTLKFIC VRGEGEGDATNGKLTLKFIC t g c g c g g a g a g g g a g a g g g c g a c g c a a c c a a t g g c a a g c t g a c a c t g a a g t t c a t c t g t a a c g c g c c t c t c c c t c t c c c g c t g c g t t g g t t a c c g t t c g a c t g t g a c t t c a a g t a g a c a t

480 (in frame with Twin-Strep-tag) TTGKLPVPWPTLVTTLTYGV TTGKLPVPWPTLVTTLTYGV c c a c a g g c a a g c t g c c t g t g c c a t g g c c c a c c c t g g t g a c c a c a c t g a c a t a c g g c g t g c g g t g t c c g t t c g a c g g a c a c g g t a c c g g g t g g g a c c a c t g g t g t g a c t g t a t g c c g c a c g

540 (in frame with Twin-Strep-tag) QCFSRYPDHMKRHDFFKSAM QCFSRYPDHMKRHDFFKSAM a g t g c t t c a g c a g g t a t c c c g a c c a c a t g a a g c g c c a c g a t t t c t t t a a g t c t g c c a t g c t c a c g a a g t c g t c c a t a g g g c t g g t g t a c t t c g c g g t g c t a a a g a a a t t c a g a c g g t a c g

Bsu36I 600 (in frame with Twin-Strep-tag) PEGYVQERTISFKDDGTYKT PEGYVQERTISFKDDGTYKT c t g a g g g c t a c g t g c a g g a g a g g a c c a t c a g c t t c a a g g a c g a t g g c a c c t a t a a g a c a c g a c t c c c g a t g c a c g t c c t c t c c t g g t a g t c g a a g t t c c t g c t a c c g t g g a t a t t c t g t g

660 (in frame with Twin-Strep-tag) RAEVKFEGDTLVNRIELKGI RAEVKFEGDTLVNRIELKGI g c g c c g a g g t g a a g t t t g a g g g c g a c a c a c t g g t g a a t c g g a t c g a g c t g a a g g g c a t c g c g c g g c t c c a c t t c a a a c t c c c g c t g t g t g a c c a c t t a g c c t a g c t c g a c t t c c c g t a g c

720 (in frame with Twin-Strep-tag) DFKEDGNILGHKLEYNFNSH DFKEDGNILGHKLEYNFNSH a c t t c a a g g a g g a t g g c a a c a t c c t g g g c c a c a a g c t g g a g t a c a a c t t c a a c t c t c a c a t g a a g t t c c t c c t a c c g t t g t a g g a c c c g g t g t t c g a c c t c a t g t t g a a g t t g a g a g t g t

780 (in frame with Twin-Strep-tag) NVYITADKQKNGIKANFKIR NVYITADKQKNGIKANFKIR a c g t g t a c a t c a c c g c c g a t a a g c a g a a g a a t g g c a t c a a g g c c a a c t t t a a g a t c a g a c t g c a c a t g t a g t g g c g g c t a t t c g t c t t c t t a c c g t a g t t c c g g t t g a a a t t c t a g t c t g

840 (in frame with Twin-Strep-tag) HNVEDGSVQLADHYQQNTPI HNVEDGSVQLADHYQQNTPI a c a a c g t g g a g g a c g g c a g c g t g c a g c t g g c a g a t c a c t a c c a g c a g a a t a c c c c a a t c g t g t t g c a c c t c c t g c c g t c g c a c g t c g a c c g t c t a g t g a t g g t c g t c t t a t g g g g t t a g c

AccI 900 (in frame with Twin-Strep-tag) GDGPVLLPDNHYLSTQSVLS GDGPVLLPDNHYLSTQSVLS g c g a c g g a c c a g t g c t g c t g c c c g a t a a c c a c t a t c t g t c t a c a c a g a g c g t g c t g t c c a c g c t g c c t g g t c a c g a c g a c g g g c t a t t g g t g a t a g a c a g a t g t g t c t c g c a c g a c a g g t

EarIEcoO109IPpuMI 960 (in frame with Twin-Strep-tag) KDPNEKRDHMVLLEFVTAAG KDPNEKRDHMVLLEFVTAAG a g g a c c c c a a c g a g a a g a g g g a t c a c a t g g t g c t g c t g g a g t t c g t g a c c g c a g c a g g a a t c c t g g g g t t g c t c t t c t c c c t a g t g t a c c a c g a c g a c c t c a a g c a c t g g c g t c g t c c t t

BmtINheI 1020 ITHGMS 1 5 ENLYFQA (in frame with TEV site) GLAGSII TEV site ITHGMSENLYFQAGLAGSII t c a c a c a c g g a a t g t c c g a g a a t c t g t a c t t c c a g g c c g g g c t a g c a g g c a g t a t t a t t a a g t g t g t g c c t t a c a g g c t c t t a g a c a t g a a g g t c c g g c c c g a t c g t c c g t c a t a a t a a t

1080 (in frame with TEV site) NETADDIVYRLTVIIDDRYE NETADDIVYRLTVIIDDRYE a c g a a a c a g c a g a c g a c a t t g t g t a c c g c c t g a c a g t g a t t a t t g a c g a c a g a t a t g a g a t g c t t t g t c g t c t g c t g t a a c a c a t g g c g g a c t g t c a c t a a t a a c t g c t g t c t a t a c t c t

1140 (in frame with TEV site) SLKNLITLRADRLEMIINDN SLKNLITLRADRLEMIINDN g c c t g a a g a a c c t g a t c a c a c t g c g g g c c g a c a g a c t g g a g a t g a t c a t c a a c g a t a a t g c g g a c t t c t t g g a c t a g t g t g a c g c c c g g c t g t c t g a c c t c t a c t a g t a g t t g c t a t t a c

BamHI 1200 (in frame with TEV site) VSTILASIGSITEIVYLTNT VSTILASIGSITEIVYLTNT t g a g c a c c a t c c t g g c c t c t a t c g g a t c c a t c a c a g a g a t c g t g t a c c t g a c c a a c a c c a a c t c g t g g t a g g a c c g g a g a t a g c c t a g g t a g t g t c t c t a g c a c a t g g a c t g g t t g t g g t

1260 (in frame with TEV site) TIEKEICPKLAEYRNWSKPQ TIEKEICPKLAEYRNWSKPQ c a a t c g a g a a g g a g a t c t g c c c c a a g c t g g c c g a g t a t a g g a a c t g g a g c a a g c c t c a g t g t t a g c t c t t c c t c t a g a c g g g g t t c g a c c g g c t c a t a t c c t t g a c c t c g t t c g g a g t c a

1320 (in frame with TEV site) CNITGFAPFSKDNSIRLSAG CNITGFAPFSKDNSIRLSAG g t a a t a t c a c a g g c t t c g c c c c c t t c a g c a a g g a c a a t t c c a t c a g g c t g t c t g c c g g a g c a t t a t a g t g t c c g a a g c g g g g g a a g t c g t t c c t g t t a a g g t a g t c c g a c a g a c g g c c t c

1380 (in frame with TEV site) GDIWVTREPYVSCDPDKCYQ GDIWVTREPYVSCDPDKCYQ g c g a t a t c t g g g t g a c c a g g g a g c c a t a c g t g t c t t g c g a c c c c g a t a a g t g t t a t c a g t c g c t a t a g a c c c a c t g g t c c c t c g g t a t g c a c a g a a c g c t g g g g c t a t t c a c a a t a g t c a

PvuIEcoNIPasI 1440 (in frame with TEV site) FALGQGTTLNNVHSNDTVHD FALGQGTTLNNVHSNDTVHD t c g c c c t g g g c c a g g g c a c c a c a c t g a a c a a t g t g c a c t c c a a c g a c a c a g t g c a c g a t c a g c g g g a c c c g g t c c c g t g g t g t g a c t t g t t a c a c g t g a g g t t g c t g t g t c a c g t g c t a g

1500 (in frame with TEV site) RTPYRTLLMNELGVPFHLGT RTPYRTLLMNELGVPFHLGT g g a c a c c a t a c a g a a c c c t g c t g a t g a a t g a g c t g g g c g t g c c c t t t c a c c t g g g c a c c a c c t g t g g t a t g t c t t g g g a c g a c t a c t t a c t c g a c c c g c a c g g g a a a g t g g a c c c g t g g t

1560 (in frame with TEV site) KQVCIAWSSSSCHDGKAWLH KQVCIAWSSSSCHDGKAWLH a g c a g g t g t g c a t c g c c t g g a g c t c c t c t a g c t g t c a c g a c g g c a a g g c c t g g c t g c a c g t c g t c c a c a c g t a g c g g a c c t c g a g g a g a t c g a c a g t g c t g c c g t t c c g g a c c g a c g t g c

1620 (in frame with TEV site) VCVTGDDENATASFIYNGRL VCVTGDDENATASFIYNGRL t g t g c g t g a c a g g c g a c g a t g a g a a c g c c a c c g c c t c c t t c a t c t a t a a t g g c c g g c t g g a c a c g c a c t g t c c g c t g c t a c t c t t g c g g t g g c g g a g g a a g t a g a t a t t a c c g g c c g a c c

1680 (in frame with TEV site) VDSIVSWSKKILRTQESECV VDSIVSWSKKILRTQESECV t g g a t t c t a t c g t g t c t t g g a g c a a g a a g a t c c t g a g a a c c c a g g a g a g c g a a t g c g t g t a c c t a a g a t a g c a c a g a a c c t c g t t c t t c t a g g a c t c t t g g g t c c t c t c g c t t a c g c a c a

BspHI 1740 (in frame with TEV site) CINGTCTVVMTDGSASGKAD CINGTCTVVMTDGSASGKAD g c a t c a a c g g c a c c t g t a c a g t g g t c a t g a c a g a c g g c t c c g c c t c t g g c a a g g c c g a t a c g t a g t t g c c g t g g a c a t g t c a c c a g t a c t g t c t g c c g a g g c g g a g a c c g t t c c g g c t a t

1800 (in frame with TEV site) TKILFIEEGKIVHTSTLSGS TKILFIEEGKIVHTSTLSGS c c a a g a t c c t g t t t a t c g a g g a g g g c a a g a t c g t g c a c a c c a g c a c a c t g a g c g g c t c c g g g t t c t a g g a c a a a t a g c t c c t c c c g t t c t a g c a c g t g t g g t c g t g t g a c t c g c c g a g g c

1860 (in frame with TEV site) AQHVEECSCYPRYPGVRCVC AQHVEECSCYPRYPGVRCVC c c c a g c a c g t g g a g g a g t g c t c c t g t t a c c c c a g g t a t c c t g g c g t g a g g t g c g t g t g c c g g g t c g t g c a c c t c c t c a c g a g g a c a a t g g g g t c c a t a g g a c c g c a c t c c a c g c a c a c g g

1920 (in frame with TEV site) RDNWKGSNRPIVDINIKDYS RDNWKGSNRPIVDINIKDYS g c g a c a a c t g g a a g g g c t c c a a t c g c c c c a t c g t g g a c a t c a a c a t c a a g g a t t a c t c t a c g c t g t t g a c c t t c c c g a g g t t a g c g g g g t a g c a c c t g t a g t t g t a g t t c c t a a t g a g a t

1980 (in frame with TEV site) IVSSYVCSGLVGDTPRKNDS IVSSYVCSGLVGDTPRKNDS t c g t g t c c t c t t a c g t g t g c a g c g g a c t g g t g g g c g a c a c c c c t a g a a a g a a t g a t a g c t a g c a c a g g a g a a t g c a c a c g t c g c c t g a c c a c c c g c t g t g g g g a t c t t t c t t a c t a t c g a

2040 (in frame with TEV site) SSSSHCLDPNNEEGGHGVKG SSSSHCLDPNNEEGGHGVKG c c t c t a g c t c c c a c t g t c t g g a c c c a a a c a a t g a g g a g g g a g g a c a c g g a g t g a a g g g a t g g a g a t c g a g g g t g a c a g a c c t g g g t t t g t t a c t c c t c c c t c c t g t g c c t c a c t t c c c t a

BmgBI 2100 (in frame with TEV site) WAFDDGNDVWMGRTISEKLR WAFDDGNDVWMGRTISEKLR g g g c c t t c g a c g a t g g c a a c g a c g t g t g g a t g g g c c g g a c a a t c t c t g a g a a g c t g a g a a c c c g g a a g c t g c t a c c g t t g c t g c a c a c c t a c c c g g c c t g t t a g a g a c t c t t c g a c t c t t

PstI 2160 (in frame with TEV site) SGYETFKVIEGWSNPNSKLQ SGYETFKVIEGWSNPNSKLQ g c g g c t a c g a g a c c t t c a a a g t g a t c g a g g g c t g g a g c a a c c c t a a t t c c a a g c t g c a g a c g c c g a t g c t c t g g a a g t t t c a c t a g c t c c c g a c c t c g t t g g g a t t a a g g t t c g a c g t c t

2220 (in frame with TEV site) INRQVIVDRGNRSGYSGIFS INRQVIVDRGNRSGYSGIFS t c a a c c g g c a g g t c a t c g t g g a t a g g g g c a a t c g c t c c g g c t a c t c t g g c a t c t t c t c c g a g t t g g c c g t c c a g t a g c a c c t a t c c c c g t t a g c g a g g c c g a t g a g a c c g t a g a a g a g g c

2280 (in frame with TEV site) VEGKSCINRCFYVELIRGRK VEGKSCINRCFYVELIRGRK t g g a g g g c a a g t c t t g c a t c a a c a g a t g t t t t t a t g t g g a g c t g a t c c g g g g c a g a a a g c a c c t c c c g t t c a g a a c g t a g t t g t c t a c a a a a a t a c a c c t c g a c t a g g c c c c g t c t t t c g

2340 (in frame with TEV site) QETEVLWTSNSIVVFCGTSG QETEVLWTSNSIVVFCGTSG a g g a g a c a g a g g t g c t g t g g a c c t c t a a t a g c a t c g t g g t g t t c t g c g g c a c c a g c g g a a t c c t c t g t c t c c a c g a c a c c t g g a g a t t a t c g t a g c a c c a c a a g a c g c c g t g g t c g c c t t

EagINotI 2400 (in frame with TEV site) TYGTGSWPDGADINLMPI* TYGTGSWPDGADINLMPI* c a t a t g g a a c c g g c t c c t g g c c a g a c g g a g c a g a t a t c a a t c t g a t g c c t a t c t g a g c g g g t a t a c c t t g g c c g a g g a c c g g t c t g c c t c g t c t a t a g t t a g a c t a c g g a t a g a c t c g c c

2460 c c g c g a c t c t a g a g g a t c t t t g t g a a g g a a c c t t a c t t c t g t g g t g t g a c a t a a t t g g a c g g c g c t g a g a t c t c c t a g a a a c a c t t c c t t g g a a t g a a g a c a c c a c a c t g t a t t a a c c t g

DraI 2520 a a a c t a c c t a c a g a g a t t t a a a a a a c c t c c c a c a c c t c c c c c t g a a c c t g a a a c a t a a a a t t t g a t g g a t g t c t c t a a a t t t t t t g g a g g g t g t g g a g g g g g a c t t g g a c t t t g t a t t t t

HpaI 2580 SV40 poly(A) signal C G T SV40pA-R MQLLLLTCLLQLIMVTNKA t g a a t g c a a t t g t t g t t g t t a a c t t g t t t a t t g c a g c t t a t a a t g g t t a c a a a t a a a g c a a c t t a c g t t a a c a a c a a c a a t t g a a c a a a t a a c g t c g a a t a t t a c c a a t g t t t a t t t c g t

2640 SV40 poly(A) signal G T G G T T T G T EBV-rev T A T C G T A G T G T T T A A A G SV40pA-R IASQISQIKHFFHCILVVVC a t a g c a t c a c a a a t t t c a c a a a t a a a g c a t t t t t t t c a c t g c a t t c t a g t t g t g g t t t g t t a t c g t a g t g t t t a a a g t g t t t a t t t c g t a a a a a a a g t g a c g t a a g a t c a a c a c c a a a c a

BspDI*ClaI* 2700 SV40 poly(A) signal C C A A A C T C A T C EBV-rev C C T T pBABE 3' PNSSMYLIMSGEDRSSDPVE c c a a a c t c a t c a a t g t a t c t t a t c a t g t c t g g g g a a g a t c g a t c t t c c g a t c c t g t g g a a g g t t t g a g t a g t t a c a t a g a a t a g t a c a g a c c c c t t c t a g c t a g a a g g c t a g g a c a c c t t

2760 SV40 promoter A C A C A C A G T C A A T C C C A pBABE 3' CVSVRVWKVPRLPSRQKYAK t g t g t g t c a g t t a g g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g a a g t a t g c a a a g a c a c a c a g t c a a t c c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c t t c a t a c g t t t c

SexAI*CsiI 2820 SV40 promoter HASQLVSNQVWKVPRLPSRQ c a t g c a t c t c a a t t a g t c a g c a a c c a g g t g t g g a a a g t c c c c a g g c t c c c c a g c a g g c a g g t a c g t a g a g t t a a t c a g t c g t t g g t c c a c a c c t t t c a g g g g t c c g a g g g g t c g t c c g t c

2880 SV40 promoter KYAKHASQLVSNHSPAPNSA a a g t a t g c a a a g c a t g c a t c t c a a t t a g t c a g c a a c c a t a g t c c c g c c c c t a a c t c c g c c t t c a t a c g t t t c g t a c g t a g a g t t a a t c a g t c g t t g g t a t c a g g g c g g g g a t t g a g g c g g

2940 SV40 promoter SV40 ori HPAPNSAQFRPFSAPWLTNF c a t c c c g c c c c t a a c t c c g c c c a g t t c c g c c c a t t c t c c g c c c c a t g g c t g a c t a a t t t t g t a g g g c g g g g a t t g a g g c g g g t c a a g g c g g g t a a g a g g c g g g g t a c c g a c t g a t t a a a a

SfiI 3000 SV40 promoter SV40 ori FYLCRGRGRLGL* t t t t a t t t a t g c a g a g g c c g a g g c c g c c t c g g c c t c t g a g c t a t t c c a g a a g t a g t g a g g a a a a t a a a t a c g t c t c c g g c t c c g g c g g a g c c g g a g a c t c g a t a a g g t c t t c a t c a c t c c

AvrII 3060 SV40 promoter SV40 ori a g g c t t t t t t g g a g g c c t a g g c t t t t g c a a a a a g c t a a t t c g g c g t a a t c t g c t g c t t g c t c c g a a a a a a c c t c c g g a t c c g a a a a c g t t t t t c g a t t a a g c c g c a t t a g a c g a c g a a c g

3120 a a a c a a a a a a a c c a c c g c t a c c a g c g g t g g t t t g t t t g c c g g a t c a a g a g c t a c c a a c t c t t t g t t t t t t t g g t g g c g a t g g t c g c c a c c a a a c a a a c g g c c t a g t t c t c g a t g g t t g a g

3180 t t t t t c c g a a g g t a a c t g g c t t c a g c a g a g c g c a g a t a c c a a a t a c t g t c c t t c t a g t g t a a a a a g g c t t c c a t t g a c c g a a g t c g t c t c g c g t c t a t g g t t t a t g a c a g g a a g a t c a c a

3240 a g c c g t a g t t a g g c c a c c a c t t c a a g a a c t c t g t a g c a c c g c c t a c a t a c c t c g c t c t g c t c g g c a t c a a t c c g g t g g t g a a g t t c t t g a g a c a t c g t g g c g g a t g t a t g g a g c g a g a c g

3300 t g a a g c c a g t t a c c a g t g g c t g c t g c c a g t g g c g a t a a g t c g t g t c t t a c c g g g t t g g a c a c t t c g g t c a a t g g t c a c c g a c g a c g g t c a c c g c t a t t c a g c a c a g a a t g g c c c a a c c t g

3360 t c a a g a g a t a g t t a c c g g a t a a g g c g c a g c g g t c g g g c t g a a c g g g g g g t t c g t g c a c a c a g t t c t c t a t c a a t g g c c t a t t c c g c g t c g c c a g c c c g a c t t g c c c c c c a a g c a c g t g t g

3420 a g c c c a g c t t g g a g c g a a c g a c c t a c a c c g a a c t g a g a t a c c t a c a g c g t g a g c t a t g a g t c g g g t c g a a c c t c g c t t g c t g g a t g t g g c t t g a c t c t a t g g a t g t c g c a c t c g a t a c t c

3480 a a a g c g c c a c g c t t c c c g a a g g g a g a a a g g c g g a c a g g t a t c c g g t a a g c g g c a g g g t c g t t t c g c g g t g c g a a g g g c t t c c c t c t t t c c g c c t g t c c a t a g g c c a t t c g c c g t c c c a g c

BssSαIBssSI 3540 G G G A A A C G C C T G G T A T C T T T pBR322ori-F g a a c a g g a g a g c g c a c g a g g g a g c t t c c a g g g g g a a a c g c c t g g t a t c t t t a t a g t c c t g c t t g t c c t c t c g c g t g c t c c c t c g a a g g t c c c c c t t t g c g g a c c a t a g a a a t a t c a g g a c

3600 t c g g g t t t c g c c a c c t c t g a c t t g a g c g t c g a t t t t t g t g a t g c t c g t c a g g g g g g c g g a a g c c c a a a g c g g t g g a g a c t g a a c t c g c a g c t a a a a a c a c t a c g a g c a g t c c c c c c g c c t

3660 f1 ori g c c t a t g g a a a a a c g c c a g c a a c g c a a g c t a g a t t t t g t t a a a a t t c g c g t t a a a t t t t t c g g a t a c c t t t t t g c g g t c g t t g c g t t c g a t c t a a a a c a a t t t t a a g c g c a a t t t a a a a a

3720 f1 ori g t t a a a t c a g c t c a t t t t t t a a c c a a t a g g c c g a a a t c g g c a a a a t c c c t t a t a a a t c a a c a a t t t a g t c g a g t a a a a a a t t g g t t a t c c g g c t t t a g c c g t t t t a g g g a a t a t t t a g t t

3780 f1 ori G G T C A A A C C T T G T T C T C A G G T G F1ori-F a a g a a t a g c c c g a g a t a g g g t t g a g t g t t g t t c c a g t t t g g a a c a a g a g t c c a c t a t t a a t t c t t a t c g g g c t c t a t c c c a a c t c a c a a c a a g g t c a a a c c t t g t t c t c a g g t g a t a a t t

3840 f1 ori a g a a c g t g g a c t c c a a c g t c a a a g g g c g a a a a a c c g t c t a t c a g g g c g a t g g c c g c c c a c t c t t g c a c c t g a g g t t g c a g t t t c c c g c t t t t t g g c a g a t a g t c c c g c t a c c g g c g g g t g

3900 f1 ori t a c g t g a a c c a t c a c c c a a a t c a a g t t t t t t g g g g t c g a g g t g c c g t a a a g c a c t a a a t c a t g c a c t t g g t a g t g g g t t t a g t t c a a a a a a c c c c a g c t c c a c g g c a t t t c g t g a t t t a g

3960 f1 ori g g a a c c c t a a a g g g a g c c c c c g a t t t a g a g c t t g a c g g g g a a a g c c g g c g a a c g t g g c g a c c t t g g g a t t t c c c t c g g g g g c t a a a t c t c g a a c t g c c c c t t t c g g c c g c t t g c a c c g c t

4020 f1 ori A G G G A A G A A A G C G A A A G G A G F1ori-R g a a a g g a a g g g a a g a a a g c g a a a g g a g c g g g c g c t a g g g c g c t g g c a a g t g t a g c g g t c a c t t t c c t t c c c t t c t t t c g c t t t c c t c g c c c g c g a t c c c g c g a c c g t t c a c a t c g c c a g t

4080 f1 ori c g c t g c g c g t a a c c a c c a c a c c c g c c g c g c t t a a t g c g c c g c t a c a g g g c g c g t a c t a t g g c g a c g c g c a t t g g t g g t g t g g g c g g c g c g a a t t a c g c g g c g a t g t c c c g c g c a t g a t a c

4140 g t t g c t t t g a c g a g c a c g t a t a a c g t g c t t t c c t c g t t a g a a t c a g a g c g g g a g c t a a a c c a a c g a a a c t g c t c g t g c a t a t t g c a c g a a a g g a g c a a t c t t a g t c t c g c c c t c g a t t t g

PshAISacII 4200 supF a g g a g g c c g a t t a a a g g g a t t t t a g a c a g g a a c g g t a c g c c a g c t g g a c c g c g g t c t t t c t c c t c c g g c t a a t t t c c c t a a a a t c t g t c c t t g c c a t g c g g t c g a c c t g g c g c c a g a a a g

4260 mature tRNA supF g g a c t t t t g a a a g t g a t g g t g g t g g g g g a a g g a t t c g a a c c t t c g a a g t c g a t g a c g g c a c c t g a a a a c t t t c a c t a c c a c c a c c c c c t t c c t a a g c t t g g a a g c t t c a g c t a c t g c c g t

4320 mature tRNA supF g a t t t a g a g t c t g c t c c c t t t g g c c g c t c g g g a a c c c c a c c a c a g g t a a t g c t t t t a c t g c t a a a t c t c a g a c g a g g g a a a c c g g c g a g c c c t t g g g g t g g t g t c c a t t a c g a a a a t g a c

4380 supF g c c t g c t c c c t t a t c g g g a a g c g g g g c g c a t c a t a t c a a a t g a c g c g c c g c t g t a a a g t g c g g a c g a g g g a a t a g c c c t t c g c c c c g c g t a g t a t a g t t t a c t g c g c g g c g a c a t t t c a c

4440 t t a c g t t g a g a a a g c t g c t c c c t g c t t g t g t g t t g g a g g t c g c t g a g t a g t g c g c g a g c a a a t g c a a c t c t t t c g a c g a g g g a c g a a c a c a c a a c c t c c a g c g a c t c a t c a c g c g c t c g t

TaqII 4500 a a a t t t a a g c t a c a a c a a g g c a a g g c t t g a c c g a c a a t t g c a t g a a g a a t c t g c t t a g g g t t t a a a t t c g a t g t t g t t c c g t t c c g a a c t g g c t g t t a a c g t a c t t c t t a g a c g a a t c c c

MluINruI 4560 CMV enhancer t t a g g c g t t t t g c g c t g c t t c g c g a t g t a c g g g c c a g a t a t a c g c g t t g a c a t t g a t t a t a a t c c g c a a a a c g c g a c g a a g c g c t a c a t g c c c g g t c t a t a t g c g c a a c t g t a a c t a a t a

AseISpeI 4620 CMV enhancer t g a c t a g t t a t t a a t a g t a a t c a a t t a c g g g g t c a t t a g t t c a t a g c c c a t a t a t g g a g t a c t g a t c a a t a a t t a t c a t t a g t t a a t g c c c c a g t a a t c a a g t a t c g g g t a t a t a c c t c a

4680 CMV enhancer t c c g c g t t a c a t a a c t t a c g g t a a a t g g c c c g c c t g g c t g a c c g c c c a a c g a c c c c c g c c a g g c g c a a t g t a t t g a a t g c c a t t t a c c g g g c g g a c c g a c t g g c g g g t t g c t g g g g g c g g

4740 CMV enhancer c a t t g a c g t c a a t a a t g a c g t a t g t t c c c a t a g t a a c g c c a a t a g g g a c t t t c c a t t g a c g t a a c t g c a g t t a t t a c t g c a t a c a a g g g t a t c a t t g c g g t t a t c c c t g a a a g g t a a c t g

4800 CMV enhancer g t c a a t g g g t g g a g t a t t t a c g g t a a a c t g c c c a c t t g g c a g t a c a t c a a g t g t a t c a t a c a g t t a c c c a c c t c a t a a a t g c c a t t t g a c g g g t g a a c c g t c a t g t a g t t c a c a t a g t a t

4860 CMV enhancer t g c c a a g t a c g c c c c c t a t t g a c g t c a a t g a c g g t a a a t g g c c c g c c t g g c a t t a t g c c c a c g g t t c a t g c g g g g g a t a a c t g c a g t t a c t g c c a t t t a c c g g g c g g a c c g t a a t a c g g g

SnaBI 4920 CMV enhancer a g t a c a t g a c c t t a t g g g a c t t t c c t a c t t g g c a g t a c a t c t a c g t a t t a g t c a t c g c t a t c a t g t a c t g g a a t a c c c t g a a a g g a t g a a c c g t c a t g t a g a t g c a t a a t c a g t a g c g a t

4980 CMV enhancer t t a c c a t g g t g a t g c g g t t t t g g c a g t a c a t c a a t g g g c g t g g a t a g c g g t t t g a c t c a c a a t g g t a c c a c t a c g c c a a a a c c g t c a t g t a g t t a c c c g c a c c t a t c g c c a a a c t g a g t g

5040 g g g g a t t t c c a a g t c t c c a c c c c a t t g a c g t c a a t g g g a g t t t g t t t t g g c a c c a a a a t c c c c c t a a a g g t t c a g a g g t g g g g t a a c t g c a g t t a c c c t c a a a c a a a a c c g t g g t t t t a g

EcoRI 5100 a a c g g g a c t t t c c a a a a t g t c g t a a c a a c t c c g c c c c a t t g a c g c a a a t g g g c g g a a t t c t t g c c c t g a a a g g t t t t a c a g c a t t g t t g a g g c g g g g t a a c t g c g t t t a c c c g c c t t a a g

5160 c t g g g c g g g a c t g g g g a g t g g c g a g c c c t c a g a t c c t g c a t a t a a g c a g c t g c t t t t t g c g a c c c g c c c t g a c c c c t c a c c g c t c g g g a g t c t a g g a c g t a t a t t c g t c g a c g a a a a a c g

5220 c t g t a c t g g g t c t c t c t g g t t a g a c c a g a t c t g a g c c t g g g a g c t c t c t g g c t a a c t a g a g a c a t g a c c c a g a g a g a c c a a t c t g g t c t a g a c t c g g a c c c t c g a g a g a c c g a t t g a t c t

HindIIIAflII 3ʹ 5ʹ 5246 g a a c c c a c t g c t t a a g c c t c a a t a a a c t t g g g t g a c g a a t t c g g a g t t a t t t

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.