Addgene IDG_KCNH4_OE_1 - Sequence Analyzer Skip to main content
Addgene

Sequence Analyzer: IDG_KCNH4_OE_1


Map View

3000 2000 1000 End (3135) SnaBI (3131) BsrGI (3077) NcoI (2891) BciVI (2841) NaeI (2644) NgoMIV (2642) XcmI (2601) PshAI (2468) BspEI - BsaWI (2460) SmaI (2044) XmaI - TspMI (2042) Bsu36I (1996) SbfI (1772) BsrDI (1625) HincII (1566) PmlI (1556) SphI (1536) NotI - EagI (1516) DraIII (1404) XmnI (1360) CsiI - SexAI * (919) EarI SapI BspQI (823) NruI (780) BstEII (723) ApoI (490) BsaBI (343) MmeI (161) SacI (155) Eco53kI (153) AccI (128) BspHI (8) Start (0) attB2 V5 tag IDG_KCNH4_OE_1 3135 bp

Sequence View

BspHIStart (0) 60 MPVMKGLLAPQNTFLDTIAT a t g c c g g t c a t g a a g g g g t t g c t g g c c c c g c a a a a c a c c t t c c t g g a c a c c a t c g c c a c c t a c g g c c a g t a c t t c c c c a a c g a c c g g g g c g t t t t g t g g a a g g a c c t g t g g t a g c g g t g g

120 RFDGTHSNFLLANAQGTRGF c g t t t t g a c g g a a c g c a c a g c a a c t t c c t g c t g g c c a a c g c a c a g g g c a c a c g g g g c t t t g c a a a a c t g c c t t g c g t g t c g t t g a a g g a c g a c c g g t t g c g t g t c c c g t g t g c c c c g a a a

MmeIEco53kISacIAccI 180 PIVYCSDGFCELTGYGRTEV c c c a t c g t c t a c t g c t c c g a c g g c t t c t g c g a g c t c a c a g g c t a c g g t c g c a c c g a g g t c g g g t a g c a g a t g a c g a g g c t g c c g a a g a c g c t c g a g t g t c c g a t g c c a g c g t g g c t c c a g

240 MQKTCSCRFLYGPETSEPAL a t g c a g a a g a c c t g c a g c t g c c g t t t c c t c t a c g g c c c a g a g a c c a g t g a g c c a g c c c t g t a c g t c t t c t g g a c g t c g a c g g c a a a g g a g a t g c c g g g t c t c t g g t c a c t c g g t c g g g a c

300 QRLHKALEGHQEHRAEICFY c a g c g t c t g c a c a a a g c c c t g g a g g g c c a c c a g g a g c a c c g g g c t g a a a t c t g c t t c t a c g t c g c a g a c g t g t t t c g g g a c c t c c c g g t g g t c c t c g t g g c c c g a c t t t a g a c g a a g a t g

BsaBI 360 RKDGSAFWCLLDMMPIKNEM c g c a a g g a t g g c t c a g c c t t t t g g t g c c t c c t g g a c a t g a t g c c c a t c a a g a a t g a g a t g g c g t t c c t a c c g a g t c g g a a a a c c a c g g a g g a c c t g t a c t a c g g g t a g t t c t t a c t c t a c

420 GEVVLFLFSFKDITQSGSPG g g g g a g g t c g t g c t g t t c c t c t t t t c c t t c a a g g a t a t c a c t c a g a g t g g a a g c c c a g g a c c c c t c c a g c a c g a c a a g g a g a a a a g g a a g t t c c t a t a g t g a g t c t c a c c t t c g g g t c c t

480 LGPQGGRGDSNHENSLGRRG c t t g g c c c c c a a g g a g g c c g c g g g g a c a g t a a t c a c g a a a a c t c c c t t g g t a g a a g g g g a g a a c c g g g g g t t c c t c c g g c g c c c c t g t c a t t a g t g c t t t t g a g g g a a c c a t c t t c c c c t

ApoI 540 ATWKFRSARRRSRTVLHRLT g c c a c c t g g a a a t t t c g g t c t g c c a g a a g a c g g a g c c g t a c t g t c c t a c a c c g a c t g a c c c g g t g g a c c t t t a a a g c c a g a c g g t c t t c t g c c t c g g c a t g a c a g g a t g t g g c t g a c t g g

600 GHFGRRGQGGMKANNNVFEP g g c c a c t t t g g c c g c c g g g g c c a g g g a g g c a t g a a g g c c a a t a a t a a c g t g t t t g a g c c a c c g g t g a a a c c g g c g g c c c c g g t c c c t c c g t a c t t c c g g t t a t t a t t g c a c a a a c t c g g t

660 KPSVPEYKVASVGGSRCLLL a a g c c a t c a g t g c c c g a g t a c a a g g t g g c c t c c g t g g g g g g g t c t c g c t g c c t c c t c c t c t t c g g t a g t c a c g g g c t c a t g t t c c a c c g g a g g c a c c c c c c c a g a g c g a c g g a g g a g g a g

720 HYSVSKAIWDGLILLATFYV c a c t a c a g c g t c t c c a a g g c c a t c t g g g a c g g c c t t a t c c t c c t t g c c a c c t t c t a c g t t g t g a t g t c g c a g a g g t t c c g g t a g a c c c t g c c g g a a t a g g a g g a a c g g t g g a a g a t g c a a

NruIBstEII 780 AVTVPYNVCFSGDDDTPITS g c g g t c a c c g t c c c c t a c a a t g t c t g t t t c t c g g g t g a c g a t g a c a c c c c c a t c a c t t c g c g c c a g t g g c a g g g g a t g t t a c a g a c a a a g a g c c c a c t g c t a c t g t g g g g g t a g t g a a g c

BspQISapIEarI 840 RHTLVSDIAVEMLFILDIIL c g a c a c a c c c t t g t c a g c g a c a t c g c c g t g g a a a t g c t c t t c a t c c t a g a t a t c a t c c t g g c t g t g t g g g a a c a g t c g c t g t a g c g g c a c c t t t a c g a g a a g t a g g a t c t a t a g t a g g a c

900 NFRTTYVSQSGQVISAPRSI a a c t t c c g c a c c a c c t a t g t g t c c c a g t c c g g c c a g g t a a t c t c t g c t c c t c g t t c c a t t t t g a a g g c g t g g t g g a t a c a c a g g g t c a g g c c g g t c c a t t a g a g a c g a g g a g c a a g g t a a

SexAI*CsiI 960 GLHYLATWFFIDLIAALPFD g g c c t c c a c t a c c t g g c c a c c t g g t t c t t c a t c g a c c t t a t t g c t g c t c t g c c c t t t g a c c c g g a g g t g a t g g a c c g g t g g a c c a a g a a g t a g c t g g a a t a a c g a c g a g a c g g g a a a c t g

1020 LLYIFNITVTSLVHLLKTVR c t g c t t t a c a t c t t c a a c a t c a c c g t g a c c t c g c t g g t g c a c c t a c t g a a g a c a g t g c g g g a c g a a a t g t a g a a g t t g t a g t g g c a c t g g a g c g a c c a c g t g g a t g a c t t c t g t c a c g c c

1080 LLRLLRLLQKLERYSQCSAV c t g t t g c g g c t g c t g c g g c t g c t g c a g a a g c t g g a g c g g t a c t c t c a g t g c a g t g c t g t g g a c a a c g c c g a c g a c g c c g a c g a c g t c t t c g a c c t c g c c a t g a g a g t c a c g t c a c g a c a c

1140 VLTLLMSVFALLAHWMACIW g t g c t c a c g c t g c t c a t g t c g g t c t t t g c g c t c c t t g c c c a c t g g a t g g c c t g c a t c t g g c a c g a g t g c g a c g a g t a c a g c c a g a a a c g c g a g g a a c g g g t g a c c t a c c g g a c g t a g a c c

1200 YVIGRREMEANDPLLWDIGW t a t g t c a t c g g g c g c c g g g a g a t g g a g g c c a a t g a c c c g c t g c t c t g g g a c a t t g g c t g g a t a c a g t a g c c c g c g g c c c t c t a c c t c c g g t t a c t g g g c g a c g a g a c c c t g t a a c c g a c c

1260 LHELGKRLEVPYVNGSVGGP t t g c a t g a g t t g g g c a a g c g t c t g g a g g t g c c c t a t g t c a a t g g c t c g g t g g g c g g c c c a a a c g t a c t c a a c c c g t t c g c a g a c c t c c a c g g g a t a c a g t t a c c g a g c c a c c c g c c g g g t

1320 SRRSAYIAALYFTLSSLTSV t c a c g g c g c a g c g c c t a c a t c g c g g c a c t g t a c t t c a c t c t a a g c a g c c t c a c c a g t g t g a g t g c c g c g t c g c g g a t g t a g c g c c g t g a c a t g a a g t g a g a t t c g t c g g a g t g g t c a c a c

XmnI 1380 GFGNVCANTDAEKIFSICTM g g c t t t g g c a a c g t g t g t g c c a a c a c c g a c g c g g a g a a g a t c t t c t c c a t c t g c a c g a t g c c g a a a c c g t t g c a c a c a c g g t t g t g g c t g c g c c t c t t c t a g a a g a g g t a g a c g t g c t a c

DraIII 1440 LIGALMHAVVFGNVTAIIQR c t c a t a g g c g c c c t g a t g c a c g c t g t g g t g t t c g g g a a c g t g a c a g c c a t c a t c c a g c g c g a g t a t c c g c g g g a c t a c g t g c g a c a c c a c a a g c c c t t g c a c t g t c g g t a g t a g g t c g c g

1500 MYSRRSLYHSRMKDLKDFIR a t g t a c t c g c g c c g c t c g c t c t a c c a c a g c c g c a t g a a g g a c c t c a a g g a c t t c a t c c g t t a c a t g a g c g c g g c g a g c g a g a t g g t g t c g g c g t a c t t c c t g g a g t t c c t g a a g t a g g c a

PmlISphIEagINotI 1560 VHRLPRPLKQRMLEYFQTTW g t g c a c c g c c t g c c g c g g c c g c t c a a g c a g c g c a t g c t c g a a t a c t t c c a g a c c a c g t g g c a c g t g g c g g a c g g c g c c g g c g a g t t c g t c g c g t a c g a g c t t a t g a a g g t c t g g t g c a c c

HincII 1620 AVNSGIDANELLRDFPDELR g c c g t c a a c a g c g g c a t c g a c g c c a a c g a g t t a c t g c g t g a c t t c c c a g a c g a g c t g a g a c g g c a g t t g t c g c c g t a g c t g c g g t t g c t c a a t g a c g c a c t g a a g g g t c t g c t c g a c t c t

BsrDI 1680 ADIAMHLNREILQLPLFGAA g c t g a c a t t g c t a t g c a c c t g a a t c g g g a g a t c c t g c a g c t g c c g t t g t t c g g g g c a g c g c g a c t g t a a c g a t a c g t g g a c t t a g c c c t c t a g g a c g t c g a c g g c a a c a a g c c c c g t c g c

1740 SRGCLRALSLHIKTSFCAPG a g c a g g g g c t g c c t g c g g g c c c t a t c g c t g c a c a t c a a g a c c t c g t t c t g c g c t c c g g g c t c g t c c c c g a c g g a c g c c c g g g a t a g c g a c g t g t a g t t c t g g a g c a a g a c g c g a g g c c c g

SbfI 1800 EYLLRRGDALQAHYYVCSGS g a g t a c c t g t t g c g c c g t g g g g a t g c c c t g c a g g c a c a t t a c t a t g t c t g c t c c g g c t c g c t c a t g g a c a a c g c g g c a c c c c t a c g g g a c g t c c g t g t a a t g a t a c a g a c g a g g c c g a g c

1860 LEVLRDNMVLAILGKGDLIG c t t g a g g t g c t c c g a g a c a a c a t g g t g c t g g c c a t c c t g g g g a a g g g g g a c c t g a t t g g a g a a c t c c a c g a g g c t c t g t t g t a c c a c g a c c g g t a g g a c c c c t t c c c c c t g g a c t a a c c t

1920 ADIPEPGQEPGLGADPNFVL g c a g a t a t c c c t g a g c c g g g g c a g g a g c c t g g g t t g g g a g c a g a c c c a a a c t t c g t g c t a c g t c t a t a g g g a c t c g g c c c c g t c c t c g g a c c c a a c c c t c g t c t g g g t t t g a a g c a c g a t

1980 KTSADVKALTYCGLQQLSSR a a g a c c a g t g c t g a t g t g a a a g c t c t g a c c t a c t g t g g c c t g c a g c a g c t g a g c a g c c g a t t c t g g t c a c g a c t a c a c t t t c g a g a c t g g a t g a c a c c g g a c g t c g t c g a c t c g t c g g c t

Bsu36I 2040 GLAEVLRLYPEYGAAFRAGL MGLPSGLAC g g g c t g g c t g a g g t c c t g a g g c t c t a t c c t g a g t a t g g g g c t g c c t t c c g g g c t g g c c t g c c c g a c c g a c t c c a g g a c t c c g a g a t a g g a c t c a t a c c c c g a c g g a a g g c c c g a c c g g a c

TspMIXmaISmaI 2100 PRDLTFNLRQGSDTSGLSRF PGTSPSTCARALTPVASAAF c c c c g g g a c c t c a c c t t c a a c c t g c g c c a g g g c t c t g a c a c c a g t g g c c t c a g c c g c t t t g g g g c c c t g g a g t g g a a g t t g g a c g c g g t c c c g a g a c t g t g g t c a c c g g a g t c g g c g a a a

2160 SRSPRLSQPRSESLGSSSDK PDPLASPSPAQKASAPPQTR t c c c g a t c c c c t c g c c t c t c c c a g c c c c g c t c a g a a a g c c t c g g c t c c t c c t c a g a c a a g a g g g c t a g g g g a g c g g a g a g g g t c g g g g c g a g t c t t t c g g a g c c g a g g a g g a g t c t g t t c

2220 TLPSITEAESGAEPGGGPRP RCHPSQRPRVARSLGVVPGP a c g c t g c c a t c c a t c a c a g a g g c c g a g a g t g g c g c g g a g c c t g g g g g t g g t c c c a g g c c c t g c g a c g g t a g g t a g t g t c t c c g g c t c t c a c c g c g c c t c g g a c c c c c a c c a g g g t c c g g g

2280 RRPLLLPNLSPARPRGSLVS DGPSCCPTSAQHGLGAPWSA c g a c g g c c c c t c c t g c t g c c c a a c c t c a g c c c a g c a c g g c c t c g g g g c t c c c t g g t c a g c g c t g c c g g g g a g g a c g a c g g g t t g g a g t c g g g t c g t g c c g g a g c c c c g a g g g a c c a g t c g

2340 LLGEELPPFSALVSSPSLSP FWARSCPHSQPLSPLLPYPH c t t t t g g g c g a g g a g c t g c c c c c a t t c t c a g c c c t t g t c t c c t c t c c t t c c t t a t c c c c a g a a a a c c c g c t c c t c g a c g g g g g t a a g a g t c g g g a a c a g a g g a g a g g a a g g a a t a g g g g t

2400 SLSPALAGQGHSASPHGPPR PCPLPWLARATVPPLTAPPG t c c c t g t c c c c t g c c c t g g c t g g c c a g g g c c a c a g t g c c t c c c c t c a c g g c c c c c c c a g g a g g g a c a g g g g a c g g g a c c g a c c g g t c c c g g t g t c a c g g a g g g g a g t g c c g g g g g g g t c c

2460 CSAAWKPPQLLIPPLGTFGP ALLPGSPLSFSFPHWEPLDL t g c t c t g c t g c c t g g a a g c c c c c t c a g c t t c t c a t t c c c c c a c t g g g a a c c t t t g g a c c t a c g a g a c g a c g g a c c t t c g g g g g a g t c g a a g a g t a a g g g g g t g a c c c t t g g a a a c c t g g a

PshAIBsaWIBspEI 2520 PDLSPRIVDGIEDSGSTAEA RTSVPG* c c g g a c c t c a g t c c c c g g a t a g t g g a t g g c a t t g a g g a c t c t g g c a g c a c a g c t g a g g c c g g c c t g g a g t c a g g g g c c t a t c a c c t a c c g t a a c t c c t g a g a c c g t c g t g t c g a c t c c g g

2580 PSFRFSRRPELPRPRSQAPP c c t t c a t t c c g a t t c a g c a g g a g g c c t g a a c t g c c a a g g c c c c g c t c c c a g g c g c c c c c t g g a a g t a a g g c t a a g t c g t c c t c c g g a c t t g a c g g t t c c g g g g c g a g g g t c c g c g g g g g a

XcmI 2640 TGTRPSPELASEAEEVKEKV a c a g g g a c c a g g c c c a g c c c a g a a t t g g c c a g t g a g g c t g a g g a g g t g a a g g a a a a g g t t t g t c c c t g g t c c g g g t c g g g t c t t a a c c g g t c a c t c c g a c t c c t c c a c t t c c t t t t c c a a

NgoMIVNaeI 2700 CRLNQEISRLNQEVSQLSRE t g c c g g c t g a a c c a g g a g a t c t c t c g t c t c a a t c a g g a g g t g t c t c a g c t t a g c c g g g a g a c g g c c g a c t t g g t c c t c t a g a g a g c a g a g t t a g t c c t c c a c a g a g t c g a a t c g g c c c t c

2760 LRHIMGLLQARLGPPGHPAG c t g c g g c a c a t c a t g g g c c t g c t g c a g g c c a g g c t g g g t c c c c c a g g c c a c c c a g c a g g c g a c g c c g t g t a g t a c c c g g a c g a c g t c c g g t c c g a c c c a g g g g g t c c g g t g g g t c g t c c g

2820 SAWTPDPPCPQLRPPCLSPC t c c g c t t g g a c c c c a g a c c c t c c t t g t c c a c a g c t g a g g c c a c c a t g c c t c t c t c c t t g t a g g c g a a c c t g g g g t c t g g g a g g a a c a g g t g t c g a c t c c g g t g g t a c g g a g a g a g g a a c a

BciVI 2880 ASRPPPSLQDTTLAEVHCPA g c g t c c a g a c c a c c a c c c a g c c t c c a g g a t a c t a c g c t t g c t g a a g t t c a c t g c c c a g c c c g c a g g t c t g g t g g t g g g t c g g a g g t c c t a t g a t g c g a a c g a c t t c a a g t g a c g g g t c g g

NcoI 2940 SVGTMETGTALLDLRPSILP a g t g t g g g g a c c a t g g a g a c a g g g a c t g c g c t c c t g g a c t t g a g a c c t t c c a t a t t g c c c t c a c a c c c c t g g t a c c t c t g t c c c t g a c g c g a g g a c c t g a a c t c t g g a a g g t a t a a c g g g

3000 PYPSEPDPLGPSPVPEASPP c c c t a c c c c t c a g a g c c t g a c c c t c t g g g a c c c t c t c c a g t g c c a g a g g c c t c a c c c c c a g g g a t g g g g a g t c t c g g a c t g g g a g a c c c t g g g a g a g g t c a c g g t c t c c g g a g t g g g g g t

3060 TPSLLRHSFQSRSDTFHSGL a c c c c a a g c c t c t t g a g g c a c a g t t t c c a g t c c a g g t c a g a c a c g t t c c a c t c a g g c c t c t g g g g t t c g g a g a a c t c c g t g t c a a a g g t c a g g t c c a g t c t g t g c a a g g t g a g t c c g g a g

BsrGI 3120 1 5 10 GKPIPNPLLG attB2 V5 tag MGPAFLYKVVGKPIPNPLLG a t g g g c c c a g c t t t c t t g t a c a a a g t g g t t g g t a a g c c t a t c c c t a a c c c t c t c c t c g g t t a c c c g g g t c g a a a g a a c a t g t t t c a c c a a c c a t t c g g a t a g g g a t t g g g a g a g g a g c c a

SnaBIEnd (3135) 3135 LDST * V5 tag LDST* c t c g a t t c t a c g t a g g a g c t a a g a t g c a t c

Restriction Enzymes

Instructions: By default, all cutters are shown. Filter on number of cut sites or search by enzyme name.

Filter

Features

Primers

BLAST

BLAST (Basic Local Alignment Search Tool) finds regions of similarity between biological sequences. Click on the buttons below to submit a BLAST search to NCBI. The results will appear in a new window. See your recent BLAST results on NCBI's website.

  • Nucleotide-Nucleotide BLAST (BLASTN)

  • Translated Nucleotide-Protein BLAST (BLASTX)

  • Sequence alignment using BLAST (BLAST2)

Sequence Analyzer Guide

Map

Displays a graphical map based on nucleotide sequence data labeled with restriction enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site)

To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations.

Sequence

Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs (theoretical open reading frames) and primers. Hovering over data labels will display additional information (e.g. cut site).

To select a portion of sequence, click one location on the sequence and then a second location to display the sequence between the two locations.

Enzymes

List of restriction enzymes that can cut a given nucleotide sequence. Table lists enzyme name and the sequence location of the cut.

Features

List of common features detected in a given nucleotide sequence. Table lists feature name, location, size, color used to indicate its position on the map, and direction (if relevant).

Primers

List of commonly used primers detected in a given nucleotide sequence. Table lists primer name, sequence, length, binding site location, and direction.

BLAST

Use Basic Local Alignment Search Tool (BLAST) via the NCBI website to determine similarity between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align a custom nucleotide sequence against a given sequence using BLAST2.

File Downloads

GenBank

File contains the nucleotide sequence and annotated features in GenBank flat file format. Open the file with a text editor or plasmid mapping software to view the sequence.

SnapGene

File contains the nucleotide sequence and enhanced annotations from SnapGene Server. Open the file with SnapGene software or the free Viewer to view the plasmid map, sequence, and perform additional sequence analysis.